 Patent Application Title |
Patent App Num. |
Date |
| Method of producing xylitol and arabinose at same time from hemicellulose hydrolysates | 20120021467 | 20120126 |
| The present invention relates to a method of producing xylitol and arabinose at same time from hemicellulose hydrolysates, material rich in xylan is hydrolyzed by acid or enzymes to obtain hydrolysate mostly containing xylose and arabinose, then inoculated in Issatchenkia orientalis S-7 and/or Issatchenkia occidentalis LJ-3 to eliminate the inhibitor of microbes, after that/or at the same, inoculated a yeast of Candida tropicalis which can consume the glucose, transform the xylose to xylitol and can not consume the arabinose, and fermented, then fermented liquor containing xylitol and arabinose is obtained. After separation, xylitol with purity more than 99% and arabinose with high purity are obtained. After concentration and crystallization respectively, crystalline xylitol and crystalline arabinose are obtained respectively.
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| Surface display of whole antibodies in eukaryotes | 20120021948 | 20120126 |
| Methods for display of recombinant whole immunoglobulins or immunoglobulin libraries on the surface of eukaryote host cells, including yeast and filamentous fungi, are described. The methods are useful for screening libraries of recombinant immunoglobulins in eukaryote host cells to identify immunoglobulins that are specific for an antigen of interest.
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| Method for culturing lactic acid bacterium and method for producing fermented milk | 20120015073 | 20120119 |
| First, prepared is a whey degradation medium to which a protease, yeast extract, and the like are added. Further, polyoxyethylene sorbitan monooleate or propylene glycol monooleate is added to the whey degradation medium. The whey degradation medium is inoculated with bacteriocin-producing lactic acid bacterium and the lactic acid bacterium is cultured while the whey degradation medium is maintained at pH of 4 to 5. After the completion of the culture, the whey degradation medium (culture solution) is centrifuged to thereby separate therefrom a concentrated cell suspension containing the lactic acid bacterium in a concentrated form. The concentrated cell suspension has very low antibacterial activity (several tens AU or less). Then, by adding the concentrated cell suspension to a yogurt mix and fermenting the same, it is... |
| Method for producing succinic acid using a yeast belonging to the genus yarrowia | 20120015415 | 20120119 |
| The present invention provides a method for producing succinic acid using a yeast belonging to the genus Yarrowia, which has been modified to reduce activity of succinate dehydrogenase in said yeast.
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| Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids | 20120015417 | 20120119 |
| The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
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| Delta-8 desaturase and its use in making polyunsaturated fatty acids | 20120015420 | 20120119 |
| Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding a delta-8 desaturase along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using this delta-8 desaturase in plants and oleaginous yeast are disclosed.
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| Method for preparing recombinant peptide from spider venom and method for relieving pain | 20120015886 | 20120119 |
| The present invention relates to a method for producing a recombinant, spider toxin peptide and analgesic compositions containing said peptide. More specifically, the present invention relates to a method in which the gene for GsMTx4 is subcloned into a vector, so that it is linked to a secretion signal sequence of the alpha factor and under the control of methanol-inducible alcohol oxidase (AOX) promoter to construct a recombinant yeast expression plasmid. Yeast cells are transformed with this plasmid to produce the GsMTx4 peptide and analgesic compositions containing said peptide. The recombinant yeast expression system of the present invention affords a more stable method for producing GsMTx4 than its natural route. Thus the GsMTx4 peptide and its derivatives produced by the method of this invention can be... |
| Controlling microbial contamination in alcoholic fermentation process | 20120009639 | 20120112 |
| The control of the microbial contamination during the sugar fermentation in the processes for obtaining alcohol is a very important action to increase the productivity of the alcoholic fermentation processes. The Saccharomyces cerevisiae yeast cells engage a very tough nutritional competition for the sugarcane juice with the bacteria (Lactobacillus sp and Acetobacter) and the wild yeasts. The proposed composition uses an antimicrobial agent of the guanidine family, such as for example, poly(hexamethyl biguanide), an antibiotic agent, and also a surfactant agent, to prevent the undesired microbial growth. The present invention further refers to the process for controlling the microbial contamination through the use of said agents.
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| Yeast ectopically expressing abnormally processed proteins and uses therefor | 20120003243 | 20120105 |
| Disclosed are yeast ectopically expressing abnormally processed proteins and methods of screening to identify compounds that modulate the function of such abnormally processed proteins in yeast. Compounds identified by such screens can be used to treat or prevent diseases associated with abnormally processed proteins or protein misfolding. Such diseases include Parkinson's Disease, Parkinson's Disease with accompanying dementia, Lewy body dementia, Alzheimer's disease with Parkinsonism, and multiple system atrophy.
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| Stable probiotic granulate, and method for preparing same | 20120003317 | 20120105 |
| The invention pertains to the field of probiotic compositions useful for human and/or animal food and health. The invention particularly relates to compositions containing stable probiotic microorganisms during the production of tablets or granules for final use. The works of the applicant of the present invention show that the mortality of probiotic microorganisms during granulation is closely linked with granulation operating conditions and with the characteristics of the selected microorganisms. Said works have led to the establishment of a mathematical expression for defining both the characteristics of the yeast as well as the parameters of the method so that the microorganism losses after granulation are lower than 1 log CFU/g of granulated food.
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| Bread product and method | 20120003354 | 20120105 |
| A leavened dough and method of its preparation are provided. The leavener can include either yeast or a chemical leavener. The dough is formed by shaking a mixture of water, flour and leavener. The dough formation does not require the use of a mechanical mixing device but can utilize only a substantially closed container that can be shaken with the flour and water inside.
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| Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris | 20120003695 | 20120105 |
| Lower eukaryotic cells such as Pichia pastoris that normally cannot use galactose as a carbon source but which have been genetically engineered according to the methods herein to use galactose as a sole source of carbon are described. The cells are genetically engineered to express several of the enzymes comprising the Leloir pathway. In particular, the cells are genetically engineered to express a galactokinase, a UDP-galactose-C4-epimerase, and a galactose-1-phosphate uridyltransferase, and optionally a galactose permease. In addition, a method is provided for improving the yield of glycoproteins that have galactose-terminated or -containing N-glycans in cells that have been genetically engineered to produce glycoproteins with N-glycans having galactose residues but which normally lack the enzymes comprising the Leloir pathway comprising transforming the cells with one or more... |
| Heterologous expression of termite cellulases yeast | 20120003701 | 20120105 |
| The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
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| Detection of micro-organisms | 20110315625 | 20111229 |
| Improved methods and devices for detecting microorganisms, such as yeast and bacteria in mixtures, are disclosed. Methods include passing a sample mixture thorough a filter device, which has been pretreated with detergent resuspending the filtrand from the filter membranes and detecting the presence of microorganisms in the filtrand.
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| Phytase-containing animal food and method | 20110318449 | 20111229 |
| A method is described for improving the nutritional value of a foodstuff comprising a source of myo-inositol hexakisphosphate by feeding the foodstuff in combination with a phytase expressed in yeast. The method comprises the step of feeding the animal the foodstuff in combination with a phytase expressed in yeast wherein the phytase can be selected from the group consisting of AppA1, AppA2 and a site-directed mutant of AppA. The invention also enables reduction of the feed to weight gain ratio and an increase bone mass and mineral content of an animal. A foodstuff and a feed additive comprising AppA2 or a site-directed mutant of AppA are also described.
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| Xylose isomerase genes and their use in fermentation of pentose sugars | 20110318790 | 20111229 |
| The present invention relates to eukaryotic cells which have the ability to isomerise xylose directly into xylulose. The cells have acquired this ability by transformation with nucleotide sequences coding for a xylose isomerase that has one or more specific sequence elements typical for isomerases having the ability of functional expression in yeasts, such as e.g. xylose isomerases obtainable from a bacterium of the genera Clostridiumand Fusobacteriumor a tunicate form the genus Ciona. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation. The may further comprise one or more genetic modifications that increase the flux of the pentose phosphate pathway, reduce unspecific aldose reductase activity, confer to the cell the ability to increase the specific xylulose... |
| Yeast organism producing isobutanol at a high yield | 20110318799 | 20111229 |
| There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and... |
| Reverse genetics of negative-strand rna viruses in yeast | 20110311581 | 20111222 |
| The present invention relates to a methodology for the generation of infectious ribonucleoparticles (RNPs) of negative-strand RNA viruses, and in particular of non-segmented negative-strand RNA viruses in yeast, especially in budding yeast. Accordingly, the patent application relates to a recombinant yeast strain suitable for the rescue of infectious non-segmented negative-strand RNA virus particles or infectious virus-like particles. The invention also relates to the use of the recombinant yeast to prepare vaccine seed and to the use of the produced RNPs or RNPs-like to prepare vaccine formulations. It also concerns the use of the recombinant yeast for the screening of libraries of DNA.
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| Formulations for animal feed comprising butyrate salt | 20110311634 | 20111222 |
| The present invention relates to formulations for animal feed comprising coated granules comprising butyrate salt; the butyrate salt is preferably the salt of sodium or calcium salt. The coated granules have a particle size of 0.1 mm or more, preferably 0.2 mm or more and about 2 mm or less, and preferably about 1 mm or less. The coated granules comprise a binder and a coating. Further, the butyrate salt can be combined with another active ingredient which may be chosen from the group consisting of plant extracts, prebiotic compounds, probiotics, yeast extracts, middle-length chain fatty acids, unsaturated long chain fatty acids, lactate salt, fat soluble vitamin, and toxin absorbing compound.
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| Production of carrier-peptide conjugates using chemically reactive unnatural amino acids | 20110312027 | 20111222 |
| Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.
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| Vectors and yeast strains for protein production | 20110312032 | 20111222 |
| Lower eukaryote host cells in which the function of at least one endogenous gene encoding a chaperone protein, such as a Protein Disulphide Isomerase (PDI), has been reduced or eliminated and at least one mammalian homolog of the chaperone protein is expressed are described. In particular aspects, the host cells further include a deletion or disruption of one or more O-protein mannosyltransferase genes, and/or overexpression of an endogenous or exogenous Ca2+ATPase. These host cells are useful for producing recombinant glycoproteins in large amounts and for producing recombinant glycoproteins that have reduced O-glycosylation.
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| Method of producing 3-mercaptohexan-1-ol and alcohol containing solution | 20110305793 | 20111215 |
| The present invention relates to a method of production of a solution containing an abundance of 3-mercaptohexan-1-ol (3MH) and alcohol and having a desirable aroma by employing a grape skin extract; an aromatic agent employing the 3MH and alcohol containing solution; a method of production of a drink with an enhanced aroma; and a method of production of fruit liquor, employing a grape skin extract. The method of production of the 3MH and alcohol containing comprising: inoculating a starting material aqueous solution comprising S-(3-hexan-1-ol)glutathione and S-(3-hexan-1-ol)-L-cysteine with a lactic acid bacterium and yeast to produce 3-mercaptohexan-1-ol and alcohol. The starting material aqueous solution comprises a grape skin extract. The lactic acid bacterium is a lactic acid bacterium that is capable of converting S-(3-hexan-1-ol)glutathione to S-(3-hexan-1-ol)-L-cysteine. After... |
| Modified industrial yeast strains | 20110305794 | 20111215 |
| The present invention relates to modified industrial yeast strains that show reduced hydrogen sulfide production. In one embodiment the invention provides an industrial yeast strain comprising a modification in a MET5 gene and/or a MET10 gene which results in reduced hydrogen sulfide production when compared to the corresponding industrial yeast strain without the modification. The present invention also relates to methods of manufacturing these modified industrial yeast strains and their use in the production of fermented products.
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| Engineering of yeast for cellulosic ethanol production | 20110306105 | 20111215 |
| The disclosure provides designer cellulosomes for efficient hydrolysis of cellulosic material and more particularly for the generating of ethanol.
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| Mutant interleukin-2 (il-2) polypeptides | 20110306752 | 20111215 |
| The present invention relates to IL-2 mutants with increased affinity for the IL-2 alpha-receptor subunit (IL-2Rα). The invention thus includes IL-2 mutants with improved biological potency. The invention also includes methods for directed evolution of IL-2α using yeast surface display to generate mutants with increased affinity for IL-2Rα.
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| Yeast screens for treatment of human disease | 20110300533 | 20111208 |
| Screening methods for identifying substances that provide therapeutic value for various diseases associated with protein misfolding are provided. Genetic and chemical screening methods are provided using a yeast system. The methods of the invention provide a rapid and cost-effective method to screen for compounds that prevent protein misfolding and/or protein fibril formation and/or protein aggregation which includes numerous neurodegenerative diseases including Parkinson's disease, Alzheimer's disease, Huntington's disease as well as non-neuronal diseases such as type 2 diabetes.
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| Method for the oral/mucosal vaccination by means of recombinant yeasts | 20110293659 | 20111201 |
| Recombinant yeast cells are produced which are used for vaccination, among other uses for the oral vaccination by feeding.
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| Recombinant der p 2 expressed in pichia pastoris as a "natural-like" allergen for immunotherapy and diagnostic purposes | 20110293665 | 20111201 |
| The present invention concerns a method for producing a recombinant Dermatophagoides pteronyssinus 2 protein (rDer p 2), comprising the steps of cultivating a Pichia pastoris yeast strain previously transformed with a rDer p 2 coding sequence, and isolating the rDer p 2 protein from said Pichia pastoris yeast strain. The invention also relates to compositions and kits comprising the rDer p 2 protein for therapeutic or diagnostic use.
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| Novel yeast strain and methods of use thereof | 20110293778 | 20111201 |
| The present disclosure relates to an isolated yeast strain deposited as NRRL Y-50184. The present disclosure also relates generally to methods of manufacturing of products, including a fermented beverage or a fermented food using yeast cell from the isolated yeast strain or a cell culture derived from the strain.
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| Novel methods of differentiating yeast strains and/or determining genetic stability of yeast strains, and uses thereof | 20110294119 | 20111201 |
| The invention relates to a method of determining the strain or strains of yeast in a sample, comprising: obtaining and screening nucleic acid from yeast for target sequences comprises all or part of a gene, or a flanking region associated with a gene, in the yeast mitochondrial DNA; and determining from the results of the screen the yeast strain or strains in the sample. Also provided is a method of determining the genetic stability of a yeast strain in a sample, wherein one target sequences in the nucleic acid comprises all or part of a gene, or a flanking region associated with a gene, in the yeast mitochondrial DNA or all or part of a gene, or a flanking region associated with a gene, located in... |
| Delta-8 desaturases and their use in making polyunsaturated fatty acids | 20110016581 | 20110120 |
| Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-8 desaturases in plants and oleaginous yeast.
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| Synthesis of long-chain polyunsaturated fatty acids by recombinant cells | 20110015415 | 20110120 |
| The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.
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| Method for high-level secretory production of protein | 20110014651 | 20110120 |
| This invention provides a means for high-level secretory production of a protein, and, in particular, a protein having a complicated structure such as an antibody, in a host cell such as a yeast cell. This invention provides a method for high-level secretory production of a foreign protein with the use of a transformed host cell having one or more types of chaperone protein genes and via suppression of O sugar chain inherent to a host cell such as a yeast cell.
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| In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris | 20110014650 | 20110120 |
| The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methylotrophic yeast such as Pichia pastoris. Methods for producing polypeptides comprising unnatural amino acids in methylotrophic yeast such as Pichia pastoris are also provided.
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| Antifungal plant proteins and methods of their use | 20110010802 | 20110113 |
| DNA constructions that provide for production of potent antifungal proteins in transgenic plants and transformed yeast cells are described. Methods of using the DNA constructs to produce transgenic plants that inhibit growth of plant pathogenic fungi are also disclosed. The use of transformed yeast cells containing the DNA constructs to produce the antifungal proteins and methods of isolating the antifungal proteins are also described.
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| Recombinant ganoderma lucidium immunomodulatory protein (rlz-8) and uses thereof | 20110009597 | 20110113 |
| Provided are the use of the recombinant Ganoderma lucidium immunomodulatory protein (rLZ-8) for the manufacturing of a medicament for antitumor, increasing leukocyte and inhibiting immunological rejection and the pharmaceutical composition comprising the rLZ-8 protein, wherein the rLZ-8 protein is expressed from pichia yeast.
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| Tangential flow filtration apparatuses, systems, and processes for the separation of compounds | 20110008866 | 20110113 |
| The invention relates to apparatuses, machines, systems and methods for the recovery and purification of proteins, peptides, nucleic acids, biologically produced polymers and other compounds from aqueous fluids. The aqueous fluids can comprise enzyme concentrates and or a fermentation broth with or without cells or other starting material. The fermentation broth can be produced by fermentations of fungal, yeast, bacterial, mammalian, insect or plant cells.
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| Compositions and methods for reverse transcription of nucleic acid molecules | 20110008770 | 20110113 |
| The present invention is generally related to compositions and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these compositions or polypeptides, particularly at temperatures above about 55° C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also relates to methods for producing Rous Sarcoma Virus (RSV) and Avian Myeloblastosis Virus (AMV) RTs or other Avian Sarcoma-Leukosis Virus (ASLV) RTs (α and/or β... |
| Methods of treating or preventing inflammatory diseases of the intestinal tract | 20110008476 | 20110113 |
| The present invention relates to a glucan derived from yeast having a beta-(1, 3)-backbone with one or more beta-(1, 3)-side chains linked thereto for use in the treatment or prevention of inflammatory bowel disease and related diseases of abnormal bowel function in an animal, in particular to such uses employing a soluble glucan, e.g. from Saccharomyces cerevisiae, preferably when administered orally. The invention also relates to alternative treatments of inflammatory bowel disease and related diseases of abnormal bowel function utilising meal or protein derived from Asteraceae.
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| Combinations of hydroxypyrion compounds and silver compounds | 20110008463 | 20110113 |
| The present invention relates to combinations of hydroxypyrion compounds, and silver compound which provide an improved biocidal effect. More particularly, the present invention relates to compositions comprising a combination of a hydroxypyrion compound selected from 1-hydroxy-2-pyridinone, ciclopirox, ciclopirox olamine, piroctone, piroctone olamine, rilopirox, or a salt thereof, together with one or more silver salts selected from silver acetate, silver alginate, silver azide, silver citrate, silver lactate, silver nitrate, silver perchlorate, silver sulfate, silver chloride, silver thiocyanate, silver-sodium-hydrogen-zirconium phosphate, silver sulfadiazine, silver cyclohexanediacetic acid and disilver 2,5-dichloro-3,6-dihydroxy-2,5-cyclohexadiene-1,4-dione in respective proportions to provide a synergistic biocidal effect. Compositions comprising these combinations are useful for the protection of any living or non-living material, such as crops, plants, fruits, seeds, objects made of wood, thatch or the like, engineering material,... |
| Production of a viable, storable worm egg suspension | 20110008390 | 20110113 |
| The present application describes a novel, advantageous purification method which guarantees the embryonation capability and the viability of a worm egg suspension. The T. suis worm eggs are first treated in a non-embryonated condition in a sulfuric acid solution at about=ph 2. Bacteria and viruses are thereby killed successfully. The germination number of yeasts and fungi is reduced. In a second step, the pH-value is increased and an orally tolerated preservative is added. Yeasts and fungi are thereby killed successfully. The suspension medium used in the second purification step can then be used as a culture medium for additional production (embryonation) and storage.
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| Process for production of xylitol | 20110003356 | 20110106 |
| The invention provides a process for producing xylitol from xylose by a yeast strain capable of converting the xylose to xylitol comprising independently growing the yeast strain in a medium; transferring the yeast strain from the medium to a feed solution, the feed solution comprising of xylose in water, and separating the xylitol from said feed solution.
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| Yeast composition and its use as cow feed additive | 20110003035 | 20110106 |
| Yeast composition for cow feed additive including at least ruminant yeast and a carrier. The carrier is selected from DDGS (corn lees), chaff, zein powder, brewery mash, bean pulp, soybeanhull, rice bran, or wheat bran. The yeast composition can alleviate the negative effects of heat stress experienced by cows during summer months and improve milk yield.
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| Surface display of recombinant proteins in lower eukaryotes | 20100331192 | 20101230 |
| Methods for display of recombinant proteins or protein libraries on the surface of lower eukaryotes such as yeast and filamentous fungi are described. The methods are useful for screening libraries of recombinant proteins in lower eukaryotes to identify particular proteins with desired properties from the array of proteins in the libraries. The methods are particularly useful for constructing and screening antibody libraries in lower eukaryotes.
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| Method for producing ethanol | 20100330641 | 20101230 |
| The present invention provides a method for producing an ethanol from a lignocellulose resource efficiently. According to the method for producing the ethanol of the present invention, an enzyme group derived from a mushroom waste substrate has a high activity and can allow cellulose or xylan in the lignocellulose resource to be efficiently converted into glucose or xylose. That is, the lignocellulose resource can be converted into a saccharified solution including the glucose or xylose thereinside. The glucose or xylose in the saccharified solution can be converted into the ethanol by fermentation of yeast or bacterium provided into the saccharified solution. The method for producing the ethanol of the present invention can allow the ethanol to be efficiently produced from the lignocellulose resource.
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| Process to produce biodiesel and/or fuel oil | 20100330615 | 20101230 |
| The present invention refers to a process to produce biodiesel and/or fuel oil from microbial oilseed and/or algal biomass and/or from sugar cane residues and derivatives. The products according to the present invention are appropriate for direct use in motors and to generate energy or steam. The integrated process of the present invention comprises the use, as raw materials, of microbial oil-producing biomass obtained from sugar cane residues and derivatives, which is integrated with algal biomass and/or glycerol and are processed by steps of production of oil-producing microbial biomass from filamentous fungi and/or yeasts, steps of simultaneous production of algal biomass by fully using residues, CO2 and residual broth of said production of microbial biomass, as well as steps of extraction and transesterification of lipids contained... |
| Combinations of anilinopyrimidines and pyrion compounds | 20100324077 | 20101223 |
| The present invention relates to combinations of an anilinopyrimidine, or a salt thereof, and a pyrion compound, or a salt thereof, which provide an improved biocidal effect. More particularly, the present invention relates to compositions comprising a combination of an anilinopyrimidine, or a salt thereof, selected from the group consisting of pyrimethanil, cyprodinil or mepanipyrim, together with a pyrion compound selected from 1-hydroxy-2-pyridinone, ciclopirox, ciclopirox olamine, piroctone, piroctone olamine, rilopirox, pyrion disulfide, sodium pyrithione and zinc pyrithione in respective proportions to provide a synergistic biocidal effect. Compositions comprising these combinations are useful for the protection of any living or non-living material, such as crops, plants, fruits, seeds, objects made of wood, thatch or the like, engineering material, biodegradable material and textiles against deterioration due to the... |
| Marker assisted directed evolution | 20100323353 | 20101223 |
| The present invention is directed at a novel method to enhance the genetic improvement of industrial microorganisms. More specifically, the present invention is directed at molecular methods for the identification of genetic variants that contribute to the genetic improvement of the microorganisms. The methods of the present invention comprise novel approaches for identifying genetic markers that linked to said genetic variants, and for using said genetic markers to select improved industrial microorganisms. In one of the embodiments, the genetic markers linked to said genetic variants are identified in microbial populations that are subjected to directed evolution. In another embodiment the genetic markers linked to said genetic variants are identified in microbial sub populations that have been prescreened for improved characteristics. The primary benefits of the use... |
| Antigen-norovirus p-domain monomers and dimers, antigen-norovirus p-particle molecules, and methods for their making and use | 20100322962 | 20101223 |
| A substituted Norovirus capsid protein monomer, having only the P-domain and called an antigen-Norovirus P-domain monomer, includes a foreign antigen inserted into one or more of three surface loops present on each P-domain monomer by molecular cloning. The antigen-P-domain monomer can assemble spontaneously into an octahedral form, called an antigen-Norovirus P-particle, that is composed of 24 copies of the antigen-P-domain monomer. Each substituted P-domain monomer will contain one to three copies of the foreign antigen, for a total of 24-72 antigen copies on each antigen-P-particle. The antigen-P-particle is useful in methods for diagnosing, immunizing and treating individuals infected with a foreign virus, for example Rotavirus, and can serve as a carrier for presentation of foreign antigens for development of novel vaccines against many infectious and non-infectious... |
| Replication-proficient dsrna capsids and uses thereof | 20100322951 | 20101223 |
| Compositions and methods useful in the production of proteins in vitro and in vivo are provided. In particular, this invention provides bionanoparticles comprised of replication-proficient, double-stranded RNA (dsRNA) capsids which are capable of transfecting, for example, target bacterial, yeast, plant and mammalian cells, and causing expression of genes of interest in said transfected cells. The bionanoparticles comprised of replication-proficient, dsRNA capsids are also capable of expressing genes of interest in vitro. The genes of interest can encode proteins that are useful, for example, as research reagents, therapeutics and vaccines. Also described are methods that enable the construction of bionanoparticles comprised of replication-proficient dsRNA capsids and methods for transfecting cells with said bionanoparticles and expressing said genes of interest in transfected cells and in vitro.
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| Combinations of phenylpyrroles and pyrion compounds | 20100319574 | 20101223 |
| Compositions comprising these combinations are useful for the protection of any living or non-living material, such as crops, plants, fruits, seeds, objects made of wood, thatch or the like, engineering material, biodegradable material and textiles against deterioration due to the action of microorganisms such as bacteria, fungi, yeasts, algae, virusses, and the like.
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| High eicosapentaenoic acid oils from improved optimized strains of yarrowia lipolytica | 20100317735 | 20101216 |
| Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom... |
| Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production | 20100317072 | 20101216 |
| Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products... |
| Methods and compositions for detecting microorganisms | 20100317020 | 20101216 |
| Methods of analyzing a sample for target microorganisms of interest are described. In particular, the methods are useful for detecting a variety of yeast and mold microorganisms based on the presence of a ubiquitous cell wall component, such as zymosan. Methods of analyzing a sample for fungal microorganisms using liposomes and/or culture media are also described.
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| Dietary supplements containing probiotics | 20100316769 | 20101216 |
| Dietary supplements comprising at least one probiotic and at least one of animal digest, dried brewers yeast, vitamin C; vitamin E, beta carotene, zinc proteinate, manganese proteinate, ferrous sulfate, copper proteinate, calcium iodate, and sodium selenite. The probiotics and other ingredients are present in the supplement in amounts sufficient to enhance the palatability of the probiotics and compositions containing the probiotics, enhance the immune system to augment the beneficial effects of the probiotics, or extend the life of the probiotics.
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| Method for producing fermented edible plants or edible animal/plants, fermented edible plants or edible animal/plants produced by same, and foods containing same | 20100316763 | 20101216 |
| The present invention relates to a method for producing fermented edible plants or edible animal/plants, to fermented edible plants or edible animal/plants produced by same, and to foods containing same. The method for producing fermented edible plants or edible animal/plants includes the steps of: producing crushed edible plants or edible animal/plants; culturing a liquid mixture of grains, saccharides, filamentous fungi, and yeast for 24 to 36 hours to produce a mixed microbial broth; inoculating the edible plants or edible animal/plants with the mixed microbial broth, and firstly fermenting the edible plants or edible animal/plants for 3 to 8 days to produce first fermented edible plants or edible animal/plants; and inoculating the first fermented edible plants or edible animal/plants with bacteria, and secondly fermenting the first fermented... |
| Agent for growing fibroblasts | 20100316664 | 20101216 |
| An agent for growing fibroblasts contains a yeast extract and a safflower extract as effective constituents. A method of growing fibroblasts comprises using a yeast extract and a safflower extract. An anti-aging agent for skin contains a yeast extract and a safflower extract as effective constituents.
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| Ethanol production by fermentation | 20100311138 | 20101209 |
| A method for producing ethanol by fermentation includes the preparation of a starter culture, inoculation of a mash with the starter culture, fermentation of the mash, and recovery of ethanol from the mash. The starter culture includes a tallow base with Chinese tallow tree parts and water which are inoculated with micro-organisms, where the micro-organisms include yeast. The micro-organisms are grown in the tallow base, and used to inoculate the mash. The mash is then fermented, and ethanol is recovered from the mash.
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| Recombinant yeast and branched alcohol production method using recombinant yeast | 20100311136 | 20101209 |
| A recombinant yeast in which a hydroxymethyl glutaryl-CoA reductase gene has been expressed to a high degree and the ADP-ribose pyrophosphatase gene and/or the yhfR gene are introduced so as to be expressed therein is provided.
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| Vectors and yeast strains for protein production | 20100311122 | 20101209 |
| Lower eukaryote host cells in which the function of at least one endogenous gene encoding a chaperone protein, such as a Protein Disulphide Isomerase (PDI), has been reduced or eliminated and at least one mammalian homolog of the chaperone protein is expressed are described. In particular aspects, the host cells further include a deletion or disruption of one or more O-protein mannosyltransferase genes, and/or overexpression of an endogenous or exogenous Ca2+ ATPase. These host cells are useful for producing recombinant glycoproteins in large amounts and for producing recombinant glycoproteins that have reduced O-glycosylation.
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| Pentacyclic alkaloid compounds and methods of use thereof | 20090325971 | 20091231 |
| The present invention relates to Pentacyclic Alkaloid Compounds, compositions comprising an effective amount of a Pentacyclic Alkaloid Compound and methods for treating or preventing cancer, a bacterial infection, a fungal infection, or a yeast infection, comprising administering to a subject in need thereof an effective amount of a Pentacyclic Alkaloid Compound. The present invention also relates to compounds and methods that are useful for making Cribrostatin IV.
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| Methods for producing ceramide using transformed yeast | 20090325247 | 20091231 |
| 2) abolishing the expression of the yeast sphinganine C4-hydroxylase gene (SUR2) by transformation of the yeast cell.
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| Sugar transport sequences, yeast strains having improved sugar uptake, and methods of use | 20090325241 | 20091231 |
| Disclosed are nucleic acid constructs comprising coding sequences operably linked to a promoter not natively associated with the coding sequence. The coding sequences encode Pichia stipitis proteins that allow recombinant strains of Saccharomyces cerevisiae expressing the protein to grow on xylose, and allow or increase uptake of xylose by Pichia stipitis or Saccharomyces cerevisiae expressing the coding sequences. Expression of the coding sequences enhances uptake of xylose and/or glucose, allowing increased ethanol or xylitol production.
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| Production of quinone derived compounds in oleaginous yeast and fungi | 20090324800 | 20091231 |
| The present invention provides systems for producing engineered oleaginous yeast or fungi that express quinone derived compounds.
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| Novel preparation of phosphodiesterase of plant origin | 20090324778 | 20091231 |
| The present invention relates to the preparation of a 5′-phosphodiesterase extracted from sorghum, and to its use for the preparation of a composition rich in 5′-nucleotides, in particular of a yeast extract.
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| Method of producing a bright, yeast fermented beverage | 20090324775 | 20091231 |
| The invention relates to a method of producing a bright, yeast fermented beverage, said method comprising the continuous production of wort from mash. More particularly, the present method comprises: a. mashing in a particulate, starch-containing raw material with water and enzymatically hydrolysing the starch to fermentable sugars; b. continuously producing a fermentable wort from the heated mash; c. introducing the wort into a fermenter to ferment the wort with the help of biologically active yeast; d. removing yeast from the fermentate by means of sedimentation; and e. clarifying the low-yeast fermentate to produce a bright, yeast fermented beverage by: processing the low-yeast fermentate in one or more separators to remove suspended material, said one or more separators being selected from the group consisting of centrifuges and... |
| Antimicrobial and sporicidal composition | 20090324737 | 20091231 |
| Germicidal compositions with enhanced activity towards killing microbiological spores and vegetative cells comprising certain quaternary ammonium compounds (QACs), phenolic compounds, monohydric alcohols, hydrogen peroxide, iodine, triclocarban, triclosan or combinations thereof with one or more spore coat opening agents. The invention also provides for the application of the germicidal compositions to animate and inanimate surfaces to help kill germs and protect against the risk of infection from bacteria, molds, yeasts, fungi, viruses, and microbiological spores.
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| Stress resistant plants | 20090320154 | 20091224 |
| Stress tolerance in plants and plant cells is achieved by using nucleotide sequences encoding enzymes involved in the NAD salvage synthesis pathway and/or the NAD de novo synthesis pathway from fungal or yeast like organisms other than Saccharomyces cereviseae, e.g., for overexpression in plants.
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| Selection of intracellular immunoglobulins | 20090317822 | 20091224 |
| A general immunoglobulin-target assay system is provided, in which a positive outcome (the generation of a signal) depends only on the intracellular interaction of immunoglobulin with target. This can be accomplished for many immunoglobulins expressed in yeast and/or in mammalian cells and allows the selection of immunoglobulins which are capable of functioning in an intracellular environment.
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| Method, composition, and device, for the treatment of amylase malfunctions / inactivity in association with saccharides (mainly polysaccharides) based diseases | 20090317371 | 20091224 |
| Physiological protein and enzyme complex having active Trimethylglycine, active amylases, active lipases, and active proteases, of human (saliva enzymes), yeast, plant, fungi, and or microbial origin, preferably digestive enzyme complex such as, Trimethylglycine, 4-α-D-glucan glucanohydrolase; Exo-1,4-α-glucosidase; Beta-fructofuranosidase; Protease (3.0); Pectinase; Lipase; Cellulase; Lactase; Malt Diastase, or digestive enzyme complex containing proteins and enzymes for the treatment of high levels of saccharides and correction of amylases malfunctioning activity or inactivity in the body. The invention also relates to the production of pharmaceutical compositions suitable for such treatment. A preferred variant of the invention relates to use of this enzyme complex having Trimethylglycine, amylolytic, lipolytic, and proteolytic activity, especially salivary, gastric, pancreatic and intestinal enzyme complex for the treatment of malfunctioning, or absent amylases enzymes activity but not... |
| Delta-8 desaturase and its use in making polyunsaturated fatty acids | 20090313720 | 20091217 |
| Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding a delta-8 desaturase along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using this delta-8 desaturase in plants and oleaginous yeast are disclosed.
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| Novel antimicrobial peptides and use thereof | 20090312265 | 20091217 |
| The invention relates to a molecule comprising at least the amino acid sequence K X K X1 X2 X K G X wherein X is any amino acid residue X1, and X2 is K or R and wherein said molecule have a length of from about 10 to about 100 amino acid residues or an analogue thereof The invention also relates to compositions comprising said molecule and use of the molecule and/or composition of the invention to combat microorganisms, such as bacteria, viruses, fungi, including yeast, and parasites.
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| Micro refinery for ethanol production | 20090311772 | 20091217 |
| A micro refinery system includes a fermentation tank, a distillation tube and a membrane ethanol separation mechanism. A batch that includes sugar, yeast and water is mixed in the fermentation tank. Sensors detect the physical characteristics of the batch and the operating status of the system. The sensors are coupled to a control system that compares the detected processing information to a look up table to determine if the system is operating under optimum ethanol productivity conditions. If the sensors detect a problem with the batch, the control system can add chemical components to the batch to correct the chemical imbalance. The system can also facilitate remote operations by transmitting sensed information to an operator computer and receiving control commands from the operator computer.
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| Method for methanol independent induction from methanol inducible promoters in pichia | 20090311749 | 20091217 |
| The present invention relates to a method for producing a polypeptide in a methylotrophic yeast host cell, wherein expression of the polypeptide is controlled by a methanol inducible promoter, comprising: i) expression of a positive regulator from a non-native promoter, said positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.
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| Colorimetric method and relative device for bacterial load detection | 20090311740 | 20091217 |
| A method for detecting a bacterial load comprising adding a sample for analysis to an analysis reagent in a sterilized reaction container, thermostatting the reaction container at a temperature of between 25 and 45° C., and verifying the change in colouring of the analysis reagent. The analysis reagent is an aqueous solution comprising amino acids chosen from the group consisting of meat peptones, vegetable peptones, casein hydrolysates, tryptose, tryptones and yeast extract; glucides chosen from monometric or oligomeric glucides metabolisable by micro-organisms; a buffer system suitable for maintaining the pH between 5.5 and 8.5; a redox indicator with potential between −250 and +250 mV and/or a pH indicator with colour change interval between pH 4.0 and pH 9.0; and a water-immiscible organic liquid compound having a... |
| Compositions and methods for diagnosis of autophagic vacuolar myopathy | 20090311704 | 20091217 |
| Transmembrane V-ATPase proton pump complexes regulate pH of extracellular space or intracellular compartments of cells. V-ATPase complexes are ubiquitous in cells across species. A human orthologue of yeast vma21, LOC203547 (VMA21), is likely involved in the assembly of the V-ATPase. Hypomorphic mutations of VMA21 are identified from XMEA patients. Methods to diagnose and/or distinguish between different forms of vacuolar or vacuolated myopathy in an individual or patient are provided based either on the sequence of the VMA21 gene and/or the level and/or activity of the V-ATPase complex. Compositions of the present invention may comprise DNA, RNA, or protein molecules corresponding to all or a portion of VMA21 and including one or more of the mutations in VMA21 identified. Cultured cells or cell lines having one or... |
| Method for identifying useful proteins of brewery yeast | 20090311680 | 20091217 |
| The invention relates to a method for identifying a useful protein of brewery yeast. More specifically, the invention relates to (a) cultivating yeast under a predetermined cultivation condition; (b) extracting a protein sample from the cultivation product of the yeast; (c) separating the protein sample by a protein separation means, selecting a target peak or spot, and recovering the target protein or a fragment thereof contained in the peak or spot; (d) determining the amino acid sequence of the target protein; (e) comparing the amino acid sequence determined in step (d) with the amino acid sequence determined in advance based on all or a part of genome sequence information of bottom fermenting yeast; (f) identifying the target protein and the gene encoding the target protein based... |
| Docosahexaenoic acid producing strains of yarrowia lipolytica | 20090311380 | 20091217 |
| An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.
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| Chemically leavened dough compositions and related methods, involving low temperature inactive yeast | 20090311375 | 20091217 |
| Described are dough compositions and methods of making dough compositions, the dough compositions being chemically leavened and being prepared from ingredients that include a low temperature inactive yeast and a yeast inhibitor; the methods include inactivating the low temperature inactive yeast to inhibit the metabolic activity of the yeast as desired; the compositions and methods can be used, e.g., to prepare refrigerated dough products.
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| Method of fermenting wort | 20090311373 | 20091217 |
| The present invention relates to a continuous method of fermenting wort, said method comprising: —fermenting wort with a biologically active yeast to produce an alcohol containing fermented liquid; —introducing the fermented liquid containing at least 10 g/l of biologically active yeast into a maturation vessel; —separately removing yeast containing sediment and supernatant liquid from the vessel; and —optionally recirculating at least a part of the yeast containing sediment to the wort fermentation; wherein the residence time of the fermented liquid in the maturation vessel exceeds 6 hours. The present method offers the advantage that it combines maturation and yeast separation into one processing step, whereas conventional continuous processes require at least two separate processing steps, one for yeast separation and one for maturation. Secondly, the present... |
| Continuous method for the production of a yeast fermented beverage | 20090311371 | 20091217 |
| The present invention provides a continuous method for the production of a yeast fermented beverage, comprising the following consecutive continuous processing steps: a. mashing starch-containing and optionally malted raw materials with aqueous liquid; b. heating the mash and enzymatically hydrolysing the starch to fermentable sugars; c. removing spent grain from the heated mash to produce a mash extract, d. converting the mash extract into wort; e. removing organic volatiles from the hot wort; f. diluting the wort with additional water; g. feeding the diluted wort into a propagation vessel in which it is combined with a recirculated stream of yeast-containing residue and in which oxygen is supplied to initiate yeast growth; h. feeding the wort from the propagation vessel into a sequence of one or more... |
| Enhanced pyruvate to acetolactate conversion in yeast | 20090305363 | 20091210 |
| A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains.
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| Phosphoadenylyl sulfate reductase gene and use thereof | 20090304858 | 20091210 |
| The present invention relates to a brewery yeast having controlled sulfite-producing capability, a process for producing alcoholic beverages with controlled sulfite amount. More particularly, the present invention relates to a yeast whose sulfite-producing capability that contribute to the product flavor is controlled by controlling the expression level of MET16 gene encoding brewery yeast phosphoadenylyl sulfate reductase Met16p, particularly non-ScMET16 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.
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| Yeast-dendritic cell vaccines and uses thereof | 20090304741 | 20091210 |
| Disclosed is a vaccine that includes a dendritic cell loaded with a yeast vehicle and antigen. Also disclosed are methods of making the vaccine and using the vaccine to elicit cellular and humoral immune responses in a mammal. Additionally, a method to elicit an immune response by administration of a yeast vehicle and an antigen that is not complexed to the yeast vehicle is disclosed.
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| Production of squalene using yeast | 20090298150 | 20091203 |
| Provided herein compositions and methods for producing isoprenoids, including squalene. In certain aspects and embodiments provided are genetically altered yeast and uses therefore. In some aspects and embodiments, the genetically altered yeast produce isoprenoids, preferably squalene. The genetically altered yeast may have alterations in the expression or activity of enzymes involved in squalene production. for example, acetyl-CoA carboxylase (or “ACCase”), HMG-CoA reductase, squalene epoxidase, and squalene synthase. One or more genes of a genetically altered yeast may be modified by gene repair oligonucleobases. Also are provided methods of producing squalene using a genetically altered yeast. The invention also provides squalene produced by genetically altered yeast.
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| Low temperature forming of feeds | 20090297664 | 20091203 |
| This application relates to a method and composition for low temperature forming of starch based and/or protein based feeds. It is particularly related to human or animal feeds containing inactivated probiotics, prebiotics, enzymes, inactivated yeasts, botanical extracts and dairy components. The low temperature extrusion process provides a means for the formation of a suitable starch and/or protein-based structure, as a carrier for temperature and/or pressure sensitive ingredients.
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| Process for producing seasoning | 20090297662 | 20091203 |
| Provided is a process for producing a seasoning having an excellent roast meat like flavor without unpleasant odor derived from yeast and burnt odor derived from saccharide. The process comprises the steps of mixing a yeast extract containing at least one of cysteine, cystine, methionine, glutathione, γ-glutamylcysteine, cystebylglycine, and salts or hydrates thereof with saccharide and/or a nucleic acid-related substance to prepare a mixture; and heating the mixture under different pH conditions.
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| Enzymatic dough conditioner and flavor improver for bakery products | 20090297659 | 20091203 |
| Novel yeast-raised and other bakery products and methods of making those products are provided. The products are formed from dough comprising very high levels of maltogenic amylase. These levels result in improved properties in the final baked product, including improved flavor, longer shelf life, and higher baked volumes. In one embodiment, the level of sugar included in the dough can be substantially reduced compared to prior art quantities, while still achieving a sweet product. The invention also allows certain chemicals such as sodium stearoyl lactylate and azodicarbonamide to be entirely eliminated from the dough.
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| O-acetylhomoserinesulfhydorelace gene and use thereof | 20090297657 | 20091203 |
| The present invention relates to a brewery yeast having controlled hydrogen sulfide-producing capability, a process for producing alcoholic beverages with controlled hydrogen sulfide amount. More particularly, the present invention relates to a yeast whose hydrogen sulfide-producing capability that increases the product flavor is controlled by enhancing the expression level of MET17 gene encoding brewery yeast O-acetylhomoserinesulfhydorelace Met17p, particularly non-ScMET17 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.
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| Manipulation of acyl-coa binding protein expression for altered lipid production in microbial hosts | 20090291479 | 20091126 |
| Acyl-CoA binding protein [“ACBP”] binds thiol esters of long fatty acids and coenzyme A in a one-to-one binding mode with high specificity and affinity. This protein is expected to play an important role in altering lipid production in oleaginous microbial organisms. Knock-out of the protein in the oleaginous yeast, Yarrowia lipolytica, results in a decrease in the total lipid content, while overexpression results in an increase in the total lipid content of the recombinant Yarrowia cells.
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| Feed, or feed additive, for livestock weight increase | 20080317900 | 20081225 |
| [Solution] The invention offers a feed composition comprising 0.01-1 wt % of koji obtained by growing Aspergillus on a substrate containing a substance anaerobically fermented by a yeast. Additionally, it offers a livestock raising method comprising feeding the livestock a feed composition obtained by adding, to a common feed composition, koji in an amount of 0.01-1 wt % with respect to the total weight of the feed composition. In particular, the koji is obtained by growing Aspergillus on a substrate containing a substance anaerobically fermented by a yeast, the Aspergillus being chosen from the group consisting of Asp. oryzae, Asp. awamori and Asp. sojae.
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| Process flavours with low acrylamide | 20080317904 | 20081225 |
| The present invention describes novel yeast extract, autolysed yeast and protein hydrolysate with an amount of free asparagine not higher than 1 mg/g. This yeast extract, autolysed yeast or protein hydrolysate may be advantageously used in the production of a process flavour with an amount of acrylamide not higher than 800 ppb. This process flavour is particularly suitable to be used in flavouring of food or feed.
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| Cultivation of micro-algae and application to animal feeds, environments, field crops, and waste treatment | 20080318304 | 20081225 |
| The present disclosure concerns producing a micro-algae product. In preferred embodiments, the method comprises collecting urine from lactating cows, mixing the collected urine with aerobically digested cow manure to form a mother liquor, fermenting the mother liquor in an algae growth tank; and forming two distinct layers of top water, including a top water layer, the top-water layer including yeast by-products and algae by-products.
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| Modified thermoplastic starch from ophiostoma ulmi polysaccharide conversion | 20080308965 | 20081218 |
| A novel modified thermoplastic starch is manufactured from a native starch using a polysaccharide produced by the fungus species Ophiostoma ulmi, by growing a culture in a yeast extract medium; adding the native starch; mixing, and harvesting the modified thermoplastic starch. The modified thermoplastic starch may be used in the manufacture of a biodegradable plastic which exhibits low water absorbency and high tensile strength. The plastic may be used to manufacture films or moulding products by casting, extrusion, injection, or compression techniques.
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| Broad spectrum non-traditional preservative system | 20080311231 | 20081218 |
| Preservative compositions containing emollient solvents such as octanediol or Symdiol™ or octoxyglycerine monoesters in combination with organic acids such as alpha hydroxyl acids can be used for preventing microbial growth and spoilage in cosmetic and topical skin formulations with the added benefit of providing silky smooth texture to skin. The compositions may optionally contain an essential oil/component. These preservative compositions are odorless and colorless and small concentrations can be added to cosmetic/topical formulations to prevent bacterial, yeast and fungal growth within 24 to 48 hours after contamination.
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| Dry powder cell culture products and methods of production thereof | 20080311660 | 20081218 |
| The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these... |
| Fibrinolysin of agkistrodon acutus venom and its usage | 20080312170 | 20081218 |
| The present invention relates to a fibrinolysin gene of Agkistrodon acutus, a vector containing the said gene and the host cell which transformed by the vector. The fibrinolysin could be used in treating diseases caused by thrombus. The fibrinolysin FII is purified from the crude venom of Agkistrodon acutus, and the corresponding gene is cloned. It is recommended to produce the fibrionolysin by the yeast expression system. The activity of the fibrionlysin is also determined. The advantages of this invention are that the expression level and activity of the fibrinolysin are both high and the quality of the fibrinolysin is stable.
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| Absorbent under-breast pad | 20080312616 | 20081218 |
| A moisture absorbent under-breast pad provides absorption of moisture between the breast and rib cage. The pad is made from 100 percent cotton cloth and is held in place by the weight of the breasts without adhesives or straps. The pad is comfortable to wear and provides relief from yeast infections, rashes, perspiration odor, and itching.
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| Satiating dietetic product | 20080299146 | 20081204 |
| The invention concerns a dietetic product belonging to the family of food products, complements or supplements for slimming or anti-obesity and appetite-suppressing or satiating food products, complements or supplements containing an efficient dose of food proteins, 70% of which at least are hydrolysed yeast proteins. The invention also concerns the use of the dietetic product in an aesthetic slimming diet. The invention further concerns the use of hydrolysed yeast proteins in the preparation of such a dietetic product.
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| Substances for reducing occurence of major cardiac events in humans | 20080299187 | 20081204 |
| A medicament comprising a dispersion of Red Yeast Rice extract in Omega-3 Oils. The medicament is supplied in capsules such that a daily dose is dispensed in an integral number of capsules. A dispersant is used, preferably Lysine and bamboo. The ratio of Red Yeast Rice Extract to EPA+DHA is in the range between about 1.4 and 2.8. The medicament reduces cholesterol, triglycerides, and reduces serious heart incidents.
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| Method for producing chlorella fermented food | 20080299257 | 20081204 |
| An object of the present invention is to provide a method for producing a chlorella fermented food in which a flavor and odor that are peculiar to algae are reduced while production of pheophorbide is suppressed. The method for producing a chlorella fermented food according to the present invention can be achieved by using a chlorella negative in coliform bacteria count, and having a content of free pheophorbide equal to or less than 18 mg % and a viable general bacteria count equal to or less than 8000 cfu/mL, or using a chlorella subjected to a heat sterilization treatment to ferment the chlorella with a baker's yeast. The baker's yeast is blended with the chlorella at a ratio of preferably 0.1 to 15% by weight. Further,... |
| Palatability enhancing compositions and foods for pets, and methods regarding the same | 20080299286 | 20081204 |
| Palatability enhancing compositions for pet foods, and pet foods with increased palatability, are provided. In some embodiments, the compositions and pet foods contain tea, such as green tea. In some embodiments, the compositions and pet foods contain brewer's yeast, liver hydrolysate, phosphate, and green tea. Shrimp meal and Tuna meal can also be utilized in these embodiments. In some embodiments, the compositions and pet foods comprise an ingredient having at least one of theanine; glutamine; tea; 1,5 octadien-3 one; 1,5-octadien-3-ol; and Camellia sinensis plant. In one example, green tea is used in a pet food palatability enhancing system at 0.1-25%, 1-15%, or 2-9% by weight of the system, and at 0.025-0.5% or 0.01 to 1.0% by weight of the pet food.
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| Malate synthase regulatory sequences for heterologous gene expression in pichia | 20080299616 | 20081204 |
| The present invention relates to the isolation of DNA regulatory regions of the malate synthase 1 gene (MLS1) from the yeast, Pichia pastoris. The present invention further relates to the expression of heterologous proteins which have been introduced into a P. pastoris host cell and can be expressed under the regulation of the MLS1 5′ regulatory promoter and MLS1 3′regulatory terminator regions. The MLS1 promoter is repressed in media containing glucose and derepressed under glucose starvation conditions or when acetate is present.
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| Microbial strains producing sphingoid bases or derivatives thereof | 20080299625 | 20081204 |
| The present invention provides microbial strains, in particular yeast strains, that produce at least 0.1 mg per g biomass dry weight of a sphingoid base. The present invention further provides a method to obtain sphingoid base-producing microbial strains comprising incubating a population of microbial cells in the presence of a suitable concentration of a toxin, selecting cells that are resistant against said toxin, and isolating cells out of the toxin-resistant cell population that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I. Optionally, the method further comprises subjecting a population of toxin-resistant microbial cells that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I to DNA-mediated transformation with a polynucleotide... |
| Ethanol fermentation process and products | 20080299630 | 20081204 |
| The present invention provides a novel process for ethanol fermentation which uses a high concentration of fresh yeast to co produce ethanol and good quality yeast.
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| Systems and processes for cellulosic ethanol production | 20080299633 | 20081204 |
| A cellulosic ethanol production process. An implementation of a process for producing fuel ethanol and biodiesel from cellulose may include: providing a raw cellulose stream by mixing a waste cellulose stream and an algae cellulose stream, hydrolyzing the raw cellulose stream to form a hydrolyzed cellulose stream, liquefying the hydrolyzed cellulose stream to produce a formed sugars stream and one or more liquefaction byproduct streams, fermenting the formed sugars stream to produce a raw ethanol stream by reacting the sugars stream with a yeast feed in at least one fermenter, separating the raw ethanol stream to form a fuel ethanol stream, producing an algae stream by reacting at least one of the one or more liquefaction byproduct streams with algae in at least one algae bioreactor,... |
| Systems and methods for large-scale production and harvesting of oil-rich algae | 20080299643 | 20081204 |
| Systems and methods for the growing of microorganisms such as algae, yeast, and bacteria are described. Seed fermentation units are associated with final fermentation ponds in various arrangements. Continuous, semicontinuous, fed batch, and batch modes of operation of the seed and final fermentations are included. Harvest methods for the cellular material and related products are described.
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| Development of novel antibiotic alternatives | 20080300169 | 20081204 |
| The present invention relates to two recombinant colicin expression systems, one utilizing a yeast expression system that produces a protein that is inexpensive to purify, and the other utilizing a plasmid expression system to be used as a probiotic culture. The recombinant colicins provide effective alternatives to conventional antibiotics and may be used to improve the efficiency of pork production, and the safety of its products.
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| Process for production of an effervescent alcoholic beverage | 20080292748 | 20081127 |
| The present invention provides a process for production of an effervescent alcoholic beverage, the process comprising: a pH adjusting step in which the pH of a yeast-containing fermentate obtained by fermenting the raw material of an effervescent alcoholic beverage with the yeast is adjusted, and a storage step in which the fermentate is aged to yield an aged liquor. According to the present invention, it is possible to produce an effervescent alcoholic beverage which has a low hydrogen sulfide concentration and an excellent flavor without using gene recombination, while avoiding adverse effects on the main fermentation step.
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| Instant food comprising flavour capsules | 20080292754 | 20081127 |
| The present invention relates to instant food products of flavor capsules that include yeast and, encapsulated therein, a flavoring ingredient or composition wherein the instant food has a water content of 13 wt % or less. The instant food may be a soup, noodles or a seasoning or topping.
... |
| Compositions and methods for establishing and/or maintaining pregnancy | 20080286382 | 20081120 |
| The present invention relates to compositions comprising selenium (e.g., organic selenium (e.g., selenized yeast (e.g., SEL-PLEX))) and methods of using the same (e.g., as a therapeutic and/or prophylactic treatment). For example, the present invention provides compositions comprising selenium (e.g., organic selenium (e.g., selenized yeast (e.g., SEL-PLEX))) and methods of using the same for treating and/or preventing one or more conditions (e.g., problems) disorders, and/or diseases related to establishing and/or maintaining a pregnancy. Compositions and methods of the present invention find use in, among other things, research and clinical (e.g., preventative and therapeutic) applications.
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| Bread and dough composition and method | 20080286411 | 20081120 |
| An enzyme fermented and yeast leavened formulation with a specified rheology can be used in a unique process to provide a high quality bread dough. The dough can be topped with a heat transfer modulator and baked to form a quality product with a bready character and a pleasing crust in a short time.
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| Delta-5 desaturase and its use in making polyunsaturated fatty acids | 20080286436 | 20081120 |
| Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this delta-5 desaturase in plants and oleaginous yeast are disclosed.
... |
| Methods for obtaining optically active epoxides and vicinal diols from 2,2-disubstituted epoxides | 20080286832 | 20081120 |
| The invention provides yeast strains, and polypeptides encoded by genes of such yeast strains, that have enantiospecific 2,2-disubstituted epoxide hydrolase activity. The invention also features nucleic acid molecules encoding such polypeptides, vectors containing such nucleic acid molecules, and cells containing such vectors. Also embraced by the invention are methods for obtaining optically active 2,2-disubstituted vicinal diols and optically active 2,2-disubstituted epoxides.
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| Expression vector, a transformant carrying the same and a method for producing heterologous protein | 20080286833 | 20081120 |
| The present invention provides an expression for use in the fission yeast Schizosaccharomyces pombe, a transformant carrying the expression vector, a method for producing a heterologous protein using the transformant, in particular a method for producing a heterologous protein in which a heat shock protein gene promoter in Schizosaccharomyces pombe is used to regulate gene expression by application of a specific form of stress, whereby the timing of the production of the desired heterologous protein can be controlled.
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| Metabolically engineered cells for the production of resveratrol or an oligomeric or glycosidically-bound derivative thereof | 20080286844 | 20081120 |
| A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.
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| Method for enriching lignocellulose residues with yeast protein | 20080286854 | 20081120 |
| The invention relates to the use of sugar cane molasses and distillery slop for carrying out a method for enriching lignocellulose residue, especially bagasse or straw, with yeast protein. The invention also relates to said lignocellulose residue enrichment method, and to the product thus obtained.
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| Yeast arrays, methods of making such arrays, and methods of analyzing such arrays | 20080287317 | 20081120 |
| This patent describes a novel method of detecting genetic interactions in yeast. This method can also be used to screen for function of biological effectors on yeast. The method encompasses crossing yeast strains with genetic alterations to acquire double mutants. The phenotypes of these double mutants are then checked to detect genetic interactions between the double mutants. This method can be used to assign function to yeast genes and their viral, prokaryotic, and eukaryotic homologs, and aptamers. It can also be used to study yeast two hybrid interactions and to find genes that regulate certain yeast promoters.
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| Composition with a fungal (yeast) lipase and method for treating lipid malabsorption in cystic fibrous as well as people suffering from pancreatic lipase insufficiency | 20080279839 | 20081113 |
| The invention provides compositions and methods for treating pancreatic enzyme insufficiency, such as the pancreatic enzyme insufficiency associated with cystic fibrosis. The invention also provides compositions comprising lipase from Candida cylindracea, alone or in combination with amylase or amyloglucosidase, protease and/or lactase. Furthermore, the invention discloses methods for treating pancreatic enzyme insufficiency comprising administering compositions to patients in need thereof.
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| Temperature control device | 20080280349 | 20081113 |
| The present invention has an object to selectively culture either one of mold and yeast, both of which are fungi, with priority. In order to attaint the object, a cell parts group 101 is heated by a heating mechanism 102 and cooled by a cooling mechanism 103. These operations are controlled by a heating-and-cooling control unit 104, and for instance, approximately 27° C. and 30 to 32° C. are switched to be adopted to a culturing temperature. Such temperature is set by a temperature-setting unit 105. By setting the culturing temperature to approximately 27° C. and 30 to 32° C., respectively, fungi and yeast will easily be selectively cultured with priority, respectively, in culture media provided in the cell parts group 101.
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| Chemical amendments for the stimulation of biogenic gas generation in deposits of carbonaceous material | 20070295505 | 20071227 |
| Methods of stimulating biogenic production of a metabolic product with enhanced hydrogen content are described. The methods may include accessing a consortium of microorganisms in a geologic formation that includes a carbonaceous material. They may also include providing a phosphorous compound to the microorganisms. The phosphorous compound stimulates the consortium to metabolize the carbonaceous material into a metabolic product with enhanced hydrogen content. Also, methods of stimulating biogenic production of a metabolic product with enhanced hydrogen content by providing a yeast extract amenment to a consortium of microorganisms is described. The yeast extract amendment stimulates the consortium to metabolize carbonaceous material in the formation into the metabolic product with enhanced hydrogen content.
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| Retarder-to-oven laminated dough | 20070298143 | 20071227 |
| A laminated dough having alternating layers of layer dough and shortening that can be frozen, stored, thawed in a retarder, and baked in an oven absent a distinct proofing step. The laminated dough includes vital wheat gluten of at least about 3% by weight of the layer dough. The elevated level of vital wheat gluten provides the layer dough with increased strength and gas holding capacity, which ultimately results in a higher baked specific volume than traditionally prepared laminated dough products. The elevated level of yeast increases the leavening rate during thawing, therefore eliminating the need for a distinct proofing step in a proof box before baking. Following baking, the resulting laminated dough product is similar in taste and visual appearance as a laminated dough product... |
| Advanced selective plating media | 20070298452 | 20071227 |
| Selective growth media N4 agars that include a combination of high levels of yeast extract, possibly higher levels of protein, elevated levels of sugar and reduced levels of sodium chloride. The combination of ingredients provides the ability to detect Salmonella spp. and Shigella spp. with almost identical sensitivity while alleviating false-negative and false-positive problems commonly encountered with the presence of Proteus spp. and Citrobacter spp.
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| Formaldehyde dehydrogenase genes from methylotrophic yeasts | 20070298500 | 20071227 |
| The present invention provides formaldehyde dehydrogenase genes (FLD) from methylotrophic yeasts. The FLD structural genes confer resistance to formaldehyde and are therefore useful as a selectable marker in methylotrophic yeasts. The FLD promoter sequences are strongly and independently induced by either methanol as sole carbon source (with ammonium sulfate as nitrogen source) or methylamine as sole nitrogen source (with glucose as carbon source). Induction under either methanol, methylamine or both provides levels of heterologous gene expression comparable to those obtained with the commonly used alcohol oxidase I gene promoter (PAOX1). The FLD promoter of Pichia pastoris (PFLD1)is an attractive alternative to PAOX1 for expression of foreign genes in P. pastoris, allowing regulation by carbon (methanol) or nitrogen (methylamine) source within the same expression strain. Yeast strains,... |
| Muteins of fibroblast growth factor 21 | 20070299007 | 20071227 |
| The present invention relates to novel muteins of human fibroblast growth factor-21 with reduced susceptibility for proteolytic degradation when expressed in yeast. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed are methods for treating type 2 diabetes, obesity, or metabolic syndrome. X-16816
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| Cell wall derivatives, their preparation process, and use thereof | 20070299034 | 20071227 |
| In a first aspect, the present invention relates to a method for isolating cell wall derivatives from fungal or yeast biomass. According to this method, chitin polymers or chitin-glucan copolymers can be obtained. In another aspect, the invention relates to a method for preparing chitosan from chitin. The invention further relates to chitin polymers, chitin-glucan polymers and chitosan polymers obtainable by the methods according to the invention. Moreover, the invention relates to the use of chitin polymers, chitin-glucan copolymers or chitosan polymers obtainable by the method according to the present invention in medical, pharmaceutical, agricultural, nutraceutical, food, textile, cosmetic, industrial and/or environmental applications, and in particular of chitin-glucan copolymers used as a technological additive for treating a food-grade liquid or in orally administered compositions.
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| Remuage-riding machine | 20070291580 | 20071220 |
| A riddling machine for intensification of depositing dead yeast cells (lees) in wine. One embodiment comprises a platform housing an engine and a transmission; supporting means, including rigid ribs, bearings on their tops, rotatably supporting a shaft; two discs mounted at the shaft's ends. A plurality of discs' holes holds cylindrical cases angularly placeable therein. The cases contain bottles with wine and lees, immovably and reversibly inserted therein. The discs are turned, revolving the cases intensifying the lees depositing to the bottlenecks. Some embodiments include vibrators actuating vibrations of the bottles, intensifying the depositing. Other embodiments have a modified expandable shaft to place the cases into the disc's holes at a predetermined angular direction common for all cases, thus tilting the platform orients all the bottles... |
| Micro-organism for decontaminating fumonisins and its use, method for decontaminating fumonisins, and feed additive containing said micro-oragnism | 20070292579 | 20071220 |
| The invention relates to a micro-organism for decontaminating fumonisins and fumonisin derivatives and to the use of bacteria or yeasts, alone or in a combination of two or more strains for decontaminating fumonisins and fumonisin derivatives in foodstuffs and/or feed. The invention also relates to a method for decontaminating fumonisins and fumonisin derivatives with the aid of a micro-organism and to a feed additive for inactivating mycotoxins, in particular fumonisins and fumonisin derivatives. The invention relates to a micro-organism for decontaminating fumonisins and fumonisin derivatives and to the use of bacteria or yeasts, alone or in a combination of two or more strains for decontaminating fumonisins and fumonisin derivatives in foodstuffs and/or feed. The invention also relates to a method for decontaminating fumonisins and fumonisin derivatives... |
| Delta 5 desaturase and its use in making polyunsaturated fatty acids | 20070292924 | 20071220 |
| The present invention relates to a Δ5 desaturase, which has the ability to convert dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) to arachidonic acid (ARA; 20:4 ω-6) and/or eicosatetraenoic acid (ETA; 20:4 ω-3) to eicosapentaenoic acid (EPA; 20:5 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this Δ5 desaturase in oleaginous yeast are disclosed.
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| Method of producing recombinant aspergillus niger beta-glucosidase and an aroma spreading plant | 20070292930 | 20071220 |
| A polypeptide having β-glucosidase enzymatic activity, a polynucleotide encoding the polypeptide, a nucleic acid constructs carrying the polynucleotide, transformed or infected cells, such as yeast cells, and transgenic organisms expressing the polynucleotide and various uses of the polypeptide, the polynucleotide, cells and/or organisms, including, producing a recombinant polypeptide having the β-glucosidase enzymatic activity, increasing the level of aroma compounds in alcoholic beverages, as well as other fermentation products of plant material, hydrolyzing cellobiose and thus increasing the level of fermentable glucose, increasing the production of alcohol, such as ethanol from plant material, increasing the aroma released from a plant or a plant product, and hydrolysis or transglycosylation of glycosides.
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| Method for extracting components from a yeast cell culture | 20070292938 | 20071220 |
| The present invention relates to a method for extracting components from yeast cells with a purified phospholipase.
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| Biologic-chemical herbicide compositions and methods of use | 20070293397 | 20071220 |
| The present invention is directed to biologic-chemical herbicide compositions (BCHs) for controlling or preventing weeds that include one or more chemical herbicides and microorganisms including gram-negative bacteria and yeast. The addition of a biological microbial component to one or more chemical herbicides significantly increases the efficacy of the chemical herbicide thereby permitting lower amounts to be used to achieve a desired level of weed control. Any chemical herbicide or combination of herbicides can be used, including fatty acid herbicides like Round-UP®. The BCHs typically also include an optional nutrient component in an amount sufficient to support the growth and replication of the microorganisms. If the BCH is applied to soil rich in nutrients, or if the crops would support the growth of the microorganisms in the... |
| Novel saperconazole crystalline forms and related processes, pharmaceutical compositions and methods | 20070293674 | 20071220 |
| The invention provides novel soluble conazole crystalline forms (e.g., cisitraconazole, saperconazole) that include salts, co-crystals and polymorphs useful as pharmaceuticals. The invention also provides pharmaceutical compositions comprising, and processes for making, these conazole crystalline forms. Methods of using such compositions for the treatment or prevention of systemic and local fungal, yeast, and dermatophyte infections are also provided.
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| Processes for detecting toxic substances | 20070287155 | 20071213 |
| Biology-based processes for detecting toxic substances are provided. The processes comprise detecting mRNA that is expressed in the presence of toxic substances by a cell comprising a yeast gene as followed, or a gene that is homologous to the yeast genes and is derived from other species, wherein the mRNA corresponds to said yeast gene or said homologous gene thereof.
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| Cryo-protective agents for microorganisms | 20070280967 | 20071206 |
| A lyophilization medium fora microorganism is provided wherein the medium is substantiality free of animal-derived products and comprises yeast extract and monosodium glutamate. The lyophilisation medium can be used for cryoprotection of strains of bacteria such as Corynebacterium diphtheriae. Method for preparing a freeze-dried culture of a microorganism using the lyophilization medium, and lyophiles of microorganisms are also provided.
... |
| Methods for obtaining optically active epoxides and vicinal diols from styrene oxides | 20070281339 | 20071206 |
| The invention provides yeast strains, and polypeptides encoded by genes of such yeast strains, that have enantiospecific styrene epoxide hydrolase activity. The invention also features nucleic acid molecules encoding such polypeptides, vectors containing such nucleic acid molecules, and cells containing such vectors. Also embraced by the invention are methods for obtaining optically active styrene vicinal diols and optically active styrene epoxides.
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| Recombinant vitellogenin enriched feed | 20070275037 | 20071129 |
| The invention provides an expression vector for expression of recombinant vitellogenin in an eukaryotic host. An eukaryotic host, including yeast comprising the expression vector according to the invention may be used as a feed or feed additive for both oviparous and non-oviparous animals, including domesticated animals. A transgenic yeast according to the invention contain increased levels of essential amino acids and fatty acids and may be used as a direct feed or fed to an intermediate live feed such as rotifers or artemias to increase the survival rates of oviparous animal or broodstock.
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| Selective withdrawal of reducing sugars during blanching | 20070275153 | 20071129 |
| The invention relates to a process of producing a food product by heat-treating a food material containing reducing sugars, comprising the step of blanching the food material, wherein the blanching step comprises subjecting the food product to an active blanching medium under blanching conditions in a blanching section to produce spent blanching medium, withdrawing reducing sugars from the spent blanching medium to produce active blanching medium using a sugar-withdrawing means, and reusing the active blanching medium. The process can be performed in one section, or the reducing sugars can be withdrawn in a desugaring section which is separated from the blanching section, recycling the treated active blanching medium to the blanching section. The process can also be applied to withdraw asparagine, or a combination of reducing... |
| Biomass conversion performance using igneous phyllosilicate minerals with high emission of far-infrared light | 20070275446 | 20071129 |
| The present invention relates to applications of far-infrared-inducing natural minerals to improve biomass conversion performance by promoting the growth of microbes, especially in the fermentation process. The present invention further relates to the enhancement of other enzyme reactions during the fermentation process. The present invention is applicable for use in corn-to-ethanol fermentation to increase the ethanol productivity as well as for yeast manufacturing.
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| Methods for obtaining optically active epoxides and vicinal diols from meso-epoxides | 20070275448 | 20071129 |
| The invention provides yeast strains, and polypeptides encoded by genes of such yeast strains, that have enantiospecific meso-epoxide hydrolase activity. The invention also features nucleic acid molecules encoding such polypeptides, vectors containing such nucleic acid molecules, and cells containing such vectors. Also embraced by the invention are methods for obtaining optically active vicinal diols from meso-epoxides.
... |
| Delta-5 desaturase and its use in making polyunsaturated fatty acids | 20070277266 | 20071129 |
| Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this delta-5 desaturase in plants and oleaginous yeast are disclosed.
... |
| Compositions and methods for reverse transcription of nucleic acid molecules | 20070269878 | 20071122 |
| The present invention is generally related to compositions and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these compositions or polypeptides, particularly at temperatures above about 55° C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also relates to methods for producing Rous Sarcoma Virus (RSV) and Avian Myeloblastosis Virus (AMV) RTs or other Avian Sarcoma-Leukosis Virus (ASLV) RTs (α and/or β... |
| Delta 5 desaturase and its use in making polyunsaturated fatty acids | 20070271632 | 20071122 |
| The present invention relates to a Δ5 desaturase, which has the ability to convert dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) to arachidonic acid (ARA; 20:4 ω-6) and/or eicosatetraenoic acid (ETA; 20:4 ω-3) to eicosapentaenoic acid (EPA; 20:5 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this Δ5 desaturase in oleaginous yeast are disclosed.
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| Binary compositions and methods for sterilization | 20070264355 | 20071115 |
| The present invention relates to binary methods and compositions comprising hypohalite (preferably hypochlorite) and peroxide (preferably hydrogen peroxide) directed to the killing of pathogenic microbes such as parasites, bacteria, fungi, yeast, and prions, the oxidation of toxins, and the preparation of potable water. The binary methods and compositions extend the microbicidal potency of conventional hypochlorite by providing additional singlet molecular oxygen generated in situ, and offer more control over reactive chlorination exposure than hypochlorite alone. This combination is a highly effective disinfecting and decontaminating agent, capable of disinfection, detoxification, or deactivation of biological contamination and many chemical toxins, facilitating the sterilizing of surfaces and solutions, and the production of potable water.
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| Beverage preservatives | 20070264401 | 20071115 |
| The present invention is directed to at least one saponin-comprising extract that can be used as a preservative and/or used as a part of a preservative system to delay, maintain, inhibit and/or reduce growth of microorganisms chosen from molds, yeasts and/or bacteria, in beverages or foods.
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| Methods and materials for the transformation of the yeast pichia ciferrii | 20070264716 | 20071115 |
| The present invention discloses a transformation system useful for the genetic engineering of the industrial yeast Pichia ciferrii. It describes the isolation of auxotrophic mutants of this yeast and nucleotide sequences able to complement the auxotrophic requirements of these strains. The transformation system is based on mutant Pichia ciferrii cells auxotrophic for uracil or lysine, which are transformed with plasmids containing the URA3 or LYS2 genes as selection markers. Novel URA3 and LYS2 genes are also disclosed.
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| Hydrolytically-resistant boron-containing therapeutics and methods of use | 20070265226 | 20071115 |
| Compositions and methods of use of boron derivatives, including benzoxaboroles, benzazaboroles and benzthiaboroles, as therapeutic agents for treatment of diseases caused by fungi, yeast, bacteria or viruses are disclosed, as well as methods for synthesis of said agents and compositions thereof.
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| New mannoprotein with full solubility in wine and its application in the stabilisation of wine | 20070259071 | 20071108 |
| The present invention describes a novel mannoprotein obtainable by a process comprising: a) subjecting a suspension of yeast cells to enzymatic hydrolysis whereby said yeast cells are degraded and mannoprotein and other yeast components are solubilised and released from the degraded yeast cells; b) recovering the solubilised mannoprotein, and optionally c) treating the recovered mannoprotein with a basic solution at a pH of at least 9. The novel mannoprotein is very soluble in wine and can be very effective in stabilising wine against tartrate precipitation or protein haze formation.
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| Frozen foodstuff | 20070259096 | 20071108 |
| Phospholipo-proteins which have been enzyme modified may be used as an emulsifier to improve the emulsion stability of frozen products, e.g., during the freeze thaw and vacuum cool processes. Especially preferred is use of the enzyme phospholipase A to modify egg yolk and to use the modified egg yolk as an emulsifier in frozen products. Creation of a more stable emulsion can be expected to improve the overall appearance of the final product both in the frozen state and after the product has been reheated. Although egg yolk is preferred, examples of other phospholipo-protein containing substances include casein, skim milk, buttermilk, whey, cream, soybean, yeast, whole egg, blood serum and wheat proteins. Egg yolk and/or other sources of phopholipo-protein can be subjected to the action of... |
| Reagent for analyzing urine and method for analyzing urine | 20070254331 | 20071101 |
| A reagent for analyzing urine is described. The reagent comprises a fungus membrane damaging agent for damaging a cellular membrane of yeast-like fungus in urine; a first dye for staining yeast-like fungus so that a fluorescent intensity of damaged yeast-like fungus becomes more intense than that of erythrocyte in urine; and a second dye for staining sperm in urine so that a fluorescent intensity of sperm becomes more intense than that of the damaged yeast-like fungus.
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| Moderating the effect of endotoxins | 20060292171 | 20061228 |
| The present invention relates to the use of an oral composition comprising yeast extract, in the manufacture of an oral composition to treat the effects of infection by pathogenic bacteria such as Clostridium difficile. Such effects may include the failure of the integrity of the gut epithelial cells and diarrhoea as well as other COX-2 mediated effects.
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| Bax-responsive genes for drug target identification in yeast and fungi | 20060286110 | 20061221 |
| The invention describes the use of nucleic acids and polypeptides which are involved in a pathway eventually leading to programmed cell death of yeast or fungi for the preparation of a medicament for treating diseases associated with yeast or fungi or for the treatment of proliferative disorders or for preventing apoptosis in certain diseases. Methods are provided to identify compounds which selectively modulate the expression or functionality of said polypeptides in the same or a parallel pathway. Also provided are compounds as well as pharmaceutical compositions, medicaments and vaccines. The invention also comprises new nucleic acid sequences, probes and primers derived thereof, expression vectors and host cells transformed with said vectors, polypeptides and antibodies raised against said polypeptides.
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| Breads comprising emulsified liquid shortening compositions comprising dietary fiber gel, water and lipid | 20060286245 | 20061221 |
| According to the present invention, fat and caloric content of breads can be reduced by the replacement of a portion fat content normally found in breads with an amount of emulsified liquid shortening composition comprising dietary fiber gel, water and lipid. The result is that fat and caloric content of breads can be manipulated with minimal effect on taste and texture. Furthermore, these emulsified mixtures, or “emulsified liquid shortening compositions comprising dietary fiber gel, water and lipid”, can further comprise functional foods such as high omega three and omega six oils and pure omega three and omega six fatty acids, medium chain triglyceride, beta carotene, calcium estearate, vitamin E, bioflavonoids, fagopyritrol, polyphenolic antioxidants of vegetable origin, lycopene, luteine and soluble fiber, for example Beta-Glucan derived from... |
| High throughput one-hybrid system | 20060286600 | 20061221 |
| The invention relates to a new high throughput yeast one-hybrid screening system.
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| Characterization of the i-spomi endonuclease from fission yeast | 20060286656 | 20061221 |
| Isolated DNAs encoding the enzyme I-SpomI and its recognition and cutting site are provided. The DNA sequences can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.
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| Animal-cell culture media comprising non-animal or plant-derived nutrients | 20060286668 | 20061221 |
| The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation or animal cells. The media comprise at least one nutrient of non-animal derivation, such as at least one plant peptide and/or at least one non-animal or plant lipid and/or fatty acid. The media may further optionally comprise an enzymatic digest or extract of yeast cells. The present invention also provides methods of cultivating animal cells in vitro using these cell culture media formulations. In addition, the media of the present invention can be used for growth of animal cells for virus production.
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| Toxin-related antibodies with antimicrobial and antiviral activity | 20060280750 | 20061214 |
| Anti-idiotypic antibodies which recognise the idiotope of an antibody specific for a yeast killer toxin possess microbicidal activity. Fragments (e.g. decapeptides) of these anti-idiotypic antibodies, particularly those comprising CDR residues, also show microbicidal activity, as do peptides having 5 the same sequence but composed of D-amino acids, or including amino acid substitutions. Peptidomimetics of these microbicidal polypeptides are also provided. Antiviral activity is also seen.
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| Composition and method for obtaining a nutritional food product using solid substrate fermentation | 20060280753 | 20061214 |
| An improved food product has an enhanced nutritional profile by utilizing solid substrate fermentation and ultraviolet irradiation. A solid substrate is mixed with water and other nutrients. The mixture is then sterilized and cooled before aseptically adding UV irradiated mushroom mycelium and maintaining the temperature so as to encourage the growth of the irradiated mycelium onto the surface of the substrate. Other materials capable of carrying out fermentation reactions can also be included such as yeast. Once colonization is complete, the mixture is heated to stop the growth process, and to begin the removal of water through evaporation. Evaporation of water continues until a sufficient moisture level is achieved such that the mixture can be further processed by milling into a fine flour type consistency. The... |
| Method and apparatus for mesoscale deposition of biological materials and biomaterials | 20060280866 | 20061214 |
| Methods and apparatus for the direct deposition or patterning of biological materials and compatible biomaterials. The method is capable of depositing biological materials and biomaterials in a computer defined pattern, and uses aerodynamic focusing of an aerosol stream to deposit mesoscale patterns onto planar or non-planar targets without the use of masks or modified environments. The aerosolized compositions may be processed before deposition (pre-processing) or after deposition on the target (post-processing). Depositable materials include, not are not limited to conductive metal precursors, nanoparticle metal inks, dielectric and resistor pastes, biocompatible polymers, and a range of biomolecules including peptides, viruses, proteinaceous enzymes, extra-cellular matrix biomolecules, as well as whole bacterial, yeast, and mammalian cell suspensions. The targets may be planar or non-planar, and are optionally biocompatible. Applications... |
| Stearoyl-coa desaturase assay | 20060281071 | 20061214 |
| A yeast-based method for identifying an agent that can modulate the activity of a human or mouse stearoyl-CoA desaturase (SCD) is disclosed. Further disclosed are substrate specificities of various human and mouse SCDs, which facilitate the identification of modulators for human and mouse SCDs.
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| Thrombin-like recombinant baxtroxobin expressed by pichia sp.and production method thereof | 20060281147 | 20061214 |
| A recombinant thrombin-like enzyme batroxobin expressed by yeast, a production method thereof, and use of the batroxobin as a hemostatic agent or antithrombotic agent is disclosed. In order to express recombinant thrombin-like enzyme, an expression vector is prepared by inserting cDNA encoding the enzyme, the transformed cell is prepared by introducing the expression vector into the cell, the transformed cell then is incubated and the recombinant thrombin-like enzyme is obtained from the cell. The recombinant thrombin-like enzyme may be usefully used as a hemostatic agent because the enzyme effectively decrease bleeding time and blood coagulation time and does not affect various blood coagulation factors. The recombinant thrombin-like enzyme is also useful for a hemostatic agent or antithrombotic agent comprising the recombinant thrombin-like enzyme as an active... |
| Saccharomyces cerevisiae yeast strain with functional expression of a glut transporter | 20060275319 | 20061207 |
| The invention relates to a strain of the yeast Saccharomyces cerevisiae which, owing to deletion of the genomic sequences, no longer synthesizes hexose transporters and, as a consequence, can no longer grow on substrates with hexoses as the only carbon source, and whose ability of growing on a substrate with a hexose as the only carbon source is restored when it expresses a GLUT4 gene.
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| Method for screening of a lipase having improved enzymatic activity using yeast surface display vector and the lipase | 20060275760 | 20061207 |
| The present invention relates to a method for screening of the lipase having improved enzymatic activity using yeast surface display vector and the mutant lipase prepared by the same, more particularly to the method comprising; 1) cloning lipase gene into surface display vector, 2) preparing mutant lipase gene library of the step 1 by mutagenic PCR, 3) transforming the mutant lipase gene library of the step 2 and surface display vector into host cell, and 4) measuring the activity of the mutant lipase displayed in the surface of the transformed host cell and selecting the mutant lipase prepared by the same. The method of the present invention can screen the lipase having improved enzymatic activity. So, it can effectively be used for the various fields, such... |
| Rapid identification of the varieties and genotypes of cryptococcus neoformans species complex using a high-throughput flow cytometer | 20060275809 | 20061207 |
| Nucleic acid probes and molecular method to identify the varieties and genotypic groups within C. neoformans species complex. The method employs a flow cytometer with a dual laser system that allows the simultaneous detection of different target sequences in a multiplex and high-throughput format. The assay uses a liquid suspension hybridization format with specific oligonucleotide probes that are covalently bound to the surface of fluorescent color-coded microspheres. Biotinylated target amplicons, which hybridized to their complementary probe sequences, are quantified by the addition of the conjugate, streptavidin-R-phycoerythrin. The assay is specific and sensitive, and allows discrimination of 1 bp mismatch with no apparent cross-reactivity and is capable of detecting 101 to 103 genome copies. The assay can be used directly with yeast cells or isolated DNA, can... |
| Composition and method for cleaning lipid deposits on contact lenses | 20060276358 | 20061207 |
| This invention is directed to an aqueous composition and methods for clean lipid deposits on contact lenses. In particular, a combination of non-ionic surfactants, branched and/or straight poly(ethylene oxide-propylene oxide) (PEO-PPO) block copolymers having HLB value greater than or equal to 18, with an additional straight poly(ethylene oxide-propylene oxide) (PEO-PPO) block copolymers having HLB value less than or equal to 15, has been found to improve the lipid cleaning properties of an aqueous composition for contact lenses and prevent the overgrowth of harmful bacteria, yeast and molds without adversely affecting the comfort or safety in terms of the level of toxicity to eye tissue.
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| Composition and method for cleaning lipid deposits on contact lenses | 20060276359 | 20061207 |
| This invention is directed to an aqueous composition and methods for cleaning lipid deposits and/or prevention of lipid deposition on contact lenses. In particular, a combination of non-ionic surfactants, straight chain poly(ethylene oxide-propylene oxide-ethylene oxide) (PEO—PPO—PEO) block copolymers having HLB value greater than or equal to 18, with an additional straight chain poly(ethylene oxide-propylene oxide-ethylene oxide) (PEO—PPO—PEO) block copolymer having an HLB value less than or equal to 11, has been found to improve the lipid cleaning properties of an aqueous composition for contact lenses and prevent the overgrowth of harmful bacteria, yeast and molds without adversely affecting the comfort or safety in terms of the level of toxicity to eye tissue.
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| Methods for production of chitin and chitosan | 20060277632 | 20061207 |
| Compositions and methods for producing chitin and chitosan are provided. The compositions comprise genetically modified organisms, including fungi, yeast, bacterial and plant organisms that have been engineered to express heterologous genes involved in chitin and chitosan synthesis. Microorganisms and plants that have been modified for production of chitin and/or chitosan within the vacuole of a cell are encompassed. Methods for production of chitin also comprise culturing the genetically engineered organisms in conditions that allow for chitin production. Further methods include converting the chitin to chitosan by a chemical process. Production of chitosan also comprises culturing organisms that are genetically modified to produce chitosan without the need for chemical modification. Methods for in vitro chitosan production are also encompassed.
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| Remedies for allergic diseases and process for producing the same | 20060269632 | 20061130 |
| Remedies for allergic diseases obtained by mixing shoots of plants belonging to the family Pinaceae with water and saccharides followed by spontaneous fermentation. As the plants belonging to the family Pinaceae, it is preferable to use plants belonging to the genus Pinus. These remedies, which enable complete recovery in a short administration time without showing any side effects, are useful as remedies for allergic diseases, in particular, asthma and atopic dermatitis. Also, a yeast isolated from these remedies for allergic diseases is provided.
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| Method for producing ergosta-5,7-dienol and/or biosynthetic intermediate and/or secondary products thereof in transgenic organisms | 20060269986 | 20061130 |
| The present invention relates to a method for the production of ergosta-5,7-dienol and/or its biosynthetic intermediates and/or metabolites by culturing genetically modified organisms, and to the genetically modified organisms, in particular yeasts, themselves.
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| Modified yeast consuming l-arabinose | 20060270008 | 20061130 |
| The present invention relates to a method for producing a L-arabinose utilizing yeast strain for the production of ethanol, whereby a yeast strain is modified by introducing and expressing araA gene (L-arabinose isomerase), araB gene (L-ribulokinase D121-N) and araD gene (L-ribulose-5-P 4-epimerase) and carrying additional mutations in its genome or overexpressing a TALl (transaldolase) gene, enabling it to consume L-arabinose, to use it as the only carbon source, and to produce ethanol, as well as a method for producing ethanol using such a modified strain.
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| Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts | 20060270010 | 20061130 |
| The present invention relates to fatty acid desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to ETA; eicosapentaenoic acid (EPA) to docosapentaenoic acid (DPA); and arachidonic acid (ARA) to EPA. Nucleic acid sequences encoding codon-optimized desaturases and elongases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the codon-optimized desaturase or elongases, and recombinant host microorganisms expressing increased levels of desaturase or elongase are described.
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| Dry powder cell culture products containing lipid and methods of production thereof | 20060270038 | 20061130 |
| The present invention relates generally to nutritive medium, medium supplement, media subgroup and buffer formulations that contain lipid. Specifically, the present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors including lipid components that facilitate the in vitro cultivation of cells. The invention is particularly directed to methods of production of these media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media... |
| Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy | 20060270045 | 20061130 |
| Methods are provided for the synthesis and secretion of recombinant proteins preferably large mammalian proteins or hetero-multimeric proteins at high levels and for prolonged time in polyploid, preferably diploid yeast. These methods use various mating competent yeast, including Pichia. In a preferred embodiment, a first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.
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| Composition to relieve side effects of alcohol intoxication | 20060263385 | 20061123 |
| A composition for the relief of symptoms of acute alcohol intoxication which includes at least one magnesium salt, at least one calcium salt, ascorbic acid, at least one sugar alcohol, at least one liver protecting botanical, at least one probiotic, at least one yeast culture and N-acetyl-L-cysteine.
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| Production of beta-glucans and mannans | 20060263415 | 20061123 |
| Disclosed are methods for producing yeast β-glucan and mannan preparations. The methods employ an autolysis process, followed by enzymatic treatment with one or more of a protease, glucanase or lipase. The preparations produced may be used in food supplements, pharmaceuticals, cosmetics, animal feeds, and neutraceuticals.
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| Use of hop acids in fuel ethanol production | 20060263484 | 20061123 |
| Six hop acids are common to hops and beer: alpha acid, beta acids, isoalpha acids, rho-isoalpha acids, tetrahydro-isoalpha acids, and hexahydro-isoalpha acids. The six hop acids were tested to determine which were the most effective in inhibiting the growth of bacteria common to fuel ethanol production. The bacteria used in the tests were Lactobacillus brevis and Lactobacillus fermentum. The minimum inhibitory concentrations (MIC) of the hop acids were determined using MRS-broth. Molasses mash and wheat mashes were used as the growth media for the fermentations. In all cases the hop acids controlled the growth of these two lactobacillus bacteria with tetrahydroisoalpha acid, hexahydroisoalpha acid, and isoalpha acid killing the most bacteria at the lowest MIC. Treating yeast propagators, steep tanks, and fermenters with a minimum inhibitory... |
| Emulsified liquid shortening compositions comprising dietary fiber gel, water and lipid | 20060263485 | 20061123 |
| Emulsified liquid shortening compositions comprising amorphous insoluble dietary fiber gel, water and lipid, as well as a method for making the compositions, are disclosed. According to the present invention, dietary fiber gel can be subjected to micro-particulation by high shear via homogenization and combined with water and lipid. These ingredients are mixed to form a mixture. The mixture can then be subjected to colloid milling or other equivalent methods of emulsification, for example homogenization and ultrasonification treatment, in the presence of food grade emulsifiers, for example lecithin, and the emulsified mixture can be pasteurized. Functional foods such as high omega three and omega six oils and pure omega three and omega six fatty acids, medium chain triglyceride, beta carotene, calcium estearate, vitamin E, bioflavonoids, fagopyritrol, polyphenolic... |
| Delivery of disease control in aquaculture and agriculture using microbes containing bioactive proteins | 20060263820 | 20061123 |
| A microbial biomass, made from algae, bacteria, fungi, yeast, or combinations thereof, provides a feed for animals raised either in agriculture or aquaculture. A feed additive, and a therapeutic composition can also be made from a microbial biomass of algae, bacteria, fungi, yeast, or combinations thereof. The feed, feed additive, and therapeutic composition can comprise one or more proteins, peptides, antibodies, antibody fragments, or a combination thereof, wherein said proteins, peptides, antibodies, antibody fragments, or a combination thereof are non-native to the microbes of the biomass. The biomass can have therapeutic, bioactive, nutritional, and/or immunogenic properties.
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| Delta-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts | 20060263866 | 20061123 |
| The present invention relates to a Δ12 fatty acid desaturase able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturase, nucleic acid sequences that hybridize thereto, DNA constructs comprising the desaturase gene, and recombinant host microorganisms expressing increased levels of the desaturase are described. Methods of increasing production of specific ω-3 and/or ω-6 fatty acids are described by overexpression of the Δ12 fatty acid desaturase or by disruption of the native gene.
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| Delta-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts | 20060263867 | 20061123 |
| The present invention relates to a Δ12 fatty acid desaturase able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturase, nucleic acid sequences that hybridize thereto, DNA constructs comprising the desaturase gene, and recombinant host microorganisms expressing increased levels of the desaturase are described. Methods of increasing production of specific ω-3 and/or ω-6 fatty acids are described by overexpression of the Δ12 fatty acid desaturase or by disruption of the native gene.
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