Disclosed herein are methods for producing genetically modified cells expressing hla-g (e. G., cell surface hla-g) persistently, and nucleic acid compositions useful for generating such genetically modified cells. Also disclosed are cell therapy methods that utilize genetically modified cells that express hla-g persistently. The hla-g genetic modifications described herein provide the cells with characteristics of reduced immunogenicity and/or improved ...

When human limbal explants are cultured according to the methods, the wnt/β-catenin signaling is inhibited, resulting in improvement of lsc proliferation, and improvement in the sternness of lscs, and thus the lscs may be obtained in high yield. These effects may lead to a higher success rate of transplantation of a limbal explant outgrowth sheet into a ...

Provided are monoclonal antibodies and antigen-binding fragments thereof that bind to, and inhibit the activity of, cd47, as well as monoclonal antibodies and antigen binding fragments thereof that compete with the former for binding to cd47. Also provided are combinations of any of the foregoing. Such antibody compounds are variously effective in 1) treating tissue ischemia and ischemia-reperfusion injury (iri) in ...

A method of radiation-free hematopoietic stem cell (hsc) transplantation comprises administering to a mammalian subject one or two doses of 2 to 10 mg/kg body weight of a purine base analog, such as 6tg as a pre-conditioning step. The method further comprises engrafting into the subject hypoxanthine-guanine phosphoribosyltransferase (hprt)-deficient donor hscs within 48 to 72 hours of the pre-conditioning step; and administering ...

The present invention provides compositions, devices, and methods for the coordinated delivery of transplant material and immune suppressors for control of transplant rejection. In particular embodiments, immune suppression cells (e. G., regulatory t cells) and transplant material (e. G., cells, tissue, etc.) are provided within a delivery scaffold for transplant into a subject.

This invention relates the use of cortisol blockers (glucocorticoid receptor [gr] antagonists) for the prevention or addiction induced anxiety and withdrawal side effects as a therapeutic and in concert with a diagnostic. Such addictions could be, but are not limited to, alcohol, drugs, caffeine, sugar, food, nicotine, etc. The present invention further relates to the use of cortisol blockers (gr ...

The present invention provides method for monitoring physiological status of an organ in a subject by monitoring morphological changes over time in transplanted tissue on an eye of the subject.

The method for preparing a tissue construct for medical purposes uses endothelial progenitor cells (epc) which have not been passaged multiple times and have a content of eoec (early outgrowth endothelial progenitor cells) and loec (late outgrowth endothelial progenitor cells). These cells and fibroblasts and/or muscle cells, viz. Myoblasts, myofibroblasts, smooth muscle cells or the progenitors thereof, are, in ...

The present invention relates to a method for producing astrocytes comprising obtaining neural progenitor cells from stem cells so as to continuously produce astrocytes with high purity and same traits, followed by two steps of differentiating the neural progenitor cells into the astrocytes, and astrocytes produced therefrom. Since the method of preparing the astrocytes provided in the present invention enables ...

The presently disclosed subject matter provides hydrogel precursor compositions (e. G., solutions) for forming three-dimensional hydrogels that support growth of physiologically relevant tissue when at least one cell is cultured in the three-dimensional hydrogel, kits comprising the hydrogel precursor composition, three-dimensional hydrogels, methods of forming the three-dimensional hydrogels, methods of growing the physiologically relevant tissue using the three-dimensional hydrogels, physiologically ...

The present invention relates to silent fc variants of anti-cd40 antibodies and compositions and methods of use of said antibodies for treating pathological disorders such as autoimmune and inflammatory disorders and/or for preventing or reducing the risk of graft rejection in transplantation.

This invention relates generally to antibodies that specifically bind toll-like receptor 4 (tlr-4), and to methods of using the anti-tlr4 antibodies as therapeutics and to methods of using the anti-tlr4 antibodies in methods of preventing transplant rejection and/or prolonging survival of transplanted biological material.

Methods of prolonging survival of allotransplanted cells, tissues or organs are presented. These methods are directed to administering to the allotransplant recipient an inhibitor of complement activity together with one or more immunosuppressants. The inhibitor of complement activity is administered chronically. These methods have been determined to aid in preventing chronic rejection of allografts. These methods can additionally be used ...

The current disclosure describes methods of expanding precursor cells for hematopoietic transplantation in subjects. The methods culture precursor cells in media containing an immobilized high molecular weight lilrb2 agonist or an lilrb2 agonist in combination with a notch agonist. The expanded cells can be used to treat a variety of hematopoietic disorders.

The invention features homo-multimers, e. G., homo-trimers, of tnfsf or tnf-like ligand muteins in which each tnfsf ligand or tnf-like ligand mutein monomer contains at least one cysteine residue substitution or insertion that promotes the formation of a disulfide bond with a cysteine residue on a neighboring tnfsf or tnf-like ligand mutein monomer. The invention features methods of producing such ...

The present disclosure describes compositions and methods for increasing the abundance of commensal bacteria belonging to the order clostridiales, including blautia, ruminococcus, clostridium, eubacterium, holdemania and dorea species, that are associated with reduced lethal gvhd and improved overall survival following bone marrow or hematopoietic stem cell transplant. The present disclosure, therefore, provides methods for reducing the likelihood, incidence or severity ...

This application relates to methods and compositions for inducing immune tolerance. Specifically, methods and uses of regulatory t cells (treg) and associated compositions for the induction of immune tolerance are described. The methods and uses may be used to prevent transplant rejection, and in the treatment of diseases or conditions such as graft versus host disease, autoimmune disease and allergies. ...

The present invention relates to the field of implants. In particular, the present invention relates to an implant for tissue reconstruction which comprises a scaffold structure that includes a void system for the generation of prevascularized connective tissue with void spaces for cell and/or tissue transplantation. Moreover, the present invention relates to a method of manufacturing such an implant, ...

The dual syringe with percutaneous cannula is a medical device for harvesting and transferring cells for autologous transplantation without the requirement of a surgical setting or ambulatory care. The dual syringe is a closed sterile system that allows for the harvesting syringe to harvest the cells/tissue from a first location of a patient which is then centrifuged to aggregate ...

A repaired ex vivo organ suitable for transplantation in a human, said repaired ex vivo organ having undergone ex vivo organ perfusion for a maintenance period, wherein said organ had been assessed as being unsuitable for transplantation into a human before the maintenance period and was determined to be suitable for transplantation after the maintenance period.

Provided is a method and a facility of plant cultivation in which leaf vegetables, fruit vegetables, and the like that are of stable quality can be cultivated at a relatively low cost and in which the period of cultivation can be stabilized. A plant cultivation method and a facility comprising a first step of raising seedlings, and a second step ...