Methods and kits are provided for determining of immunoglobulin isotypes and subclasses in a subject. In general the subject is a human who is a transplant candidate recipient or recipient, has allergies, or has an autoimmune disease. The method involves analyzing a sample of a body fluid of a transplant candidate or recipient, allergy patient or autoimmune disease sufferer and ...

The present disclosure relates to antibodies directed to cd25 and uses of such antibodies, for example to suppress organ transplant rejection or to treat multiple sclerosis.

The invention provides natural multi-composite bone implants such as bone-connective tissue-bone and osteochondral implants for the replacement and/or repair of, for example and in particular a damaged or defective bone-meniscus-bone joint or a bone-patella tendon-bone joint or osteochondral lesions, methods of preparing the composites and uses thereof. The invention also provides natural or native composite bone-connective tissue-bone and osteochondral ...

An example method of diagnosing chronic kidney disease (ckd) includes obtaining an electronic medical record for a patient having medical data. The medical data includes an indication if the patient had been previously diagnosed with ckd, an indication if the patient had previously undergone a kidney transplant, an indication if the patient had previously undergone a renal dialysis procedure, an ...

Aspects of the present invention include methods for determining a transplant category of a subject having a transplant. Common mechanisms of rejection injury are uncovered across different tissue transplants, and provide a means to understand rational drug design. Various sources of tissues are examined form the patient for understanding ar mechanism (graft biopsy), as well as monitoring by minimal invasive ...

This present disclosure relates to the use of donor organ-derived microvesicles to monitor the status of a transplanted organ in a subject. Accordingly, this disclosure provides for methods and kits for isolating, purifying and/or identifying donor organ-derived microvesicles from a biological sample of a subject. In certain embodiments, a method for isolating, purifying and/or identifying donor organ-derived microvesicles ...

Described are cell culture solutions and systems for epithelial stem cell and organoid cultures, formation of epithelial constructs and uses of the same in transplantation.

Compositions and methods are provided for modulating the growth, development and repair of bone, cartilage or other connective tissue. Devices and stimulus waveforms are provided to differentially modulate the behavior of osteoblasts, chondrocytes and other connective tissue cells to promote proliferation, differentiation, matrix formation or mineralization for in vitro or in vivo applications. Continuous-mode and pulse-burst-mode stimulation of cells with ...

Photoreceptor scaffolds and scaffold systems including the photoreceptor scaffolds are described herein. The scaffolds and scaffold systems can be used for transplantation of organized photoreceptor tissue, with or without rpe, which may improve grafted cell survival, integration, and functional visual rescue. Particularly, the photoreceptor scaffold is structured from a biocompatible film, patterned with an array of unique through-holes having a ...

Covalently modified alginate polymers, possessing enhanced biocompatibility and tailored physiochemical properties, as well as methods of making and use thereof, are disclosed herein. The covalently modified alginates are useful as a matrix for coating of any material where reduced fibrosis is desired, such as encapsulated cells for transplantation and medical devices implanted or used in the body.

The invention relates to an isolated anti-cd45rc antibody for use in preventing or treating transplant rejection, autoimmune diseases, unwanted immune responses against proteins expressed in the course of gene therapy and/or therapeutic proteins, allergy as well as lymphoma or cancer which are associated with cd45rc+ cells. The invention relates to an isolated anti-cd45rc antibody for use ...

It is intended to provide a therapeutic and/or prophylactic agent for transplant rejections, immunological diseases, allergic diseases, inflammatory diseases, thrombosis, cancers, etc., targeting human orai1. The present invention provides, for example, a pharmaceutical composition comprising an antibody that specifically recognizes human orai1 and has the activity of inhibiting human t cell activation.

Provided are compositions and methods for preventing premature follicle activation and loss induced by transplantation of ovaries or tissues derived from ovaries, thereby preserving fertility in a subject.

The present invention provides compositions and methods of using placental stem cells that originate from a post-partum placenta with conventional cord blood compositions or other stem or progenitor cells. The placental stem cells can be used alone or in a mixture with other stem cell populations. In accordance with the present invention, the placental stem cells may be mixed with ...

The present invention provides a clinically applicable method of stem cell transplantation that facilitates engraftment and reconstitutes immunocompetence of the recipient without requiring radiotherapy or chemotherapy, and without development of gvhd or graft rejection. Aspects of the present invention are based on the discovery that the depletion of the endogenous stem cell niche facilitates efficient engraftment of stem cells into ...

The dual syringe with percutaneous cannula is a medical device for harvesting and transferring cells for autologous transplantation without the requirement of a surgical setting or ambulatory care. The dual syringe is a closed sterile system that allows for the harvesting syringe to harvest the cells/tissue from a first location of a patient which is then centrifuged to aggregate ...

An apparatus, a system, and methods for maintaining and monitoring an excised donor heart. The apparatus comprises a first component for receiving and submerging therein an excised heart in a constantly circulating perfusate solution and a second component comprising equipment for adjusting the temperature and oxygen content of the perfusate solution. The first component comprises an integral pair of defibrillating ...

Methods for stabilizing blood samples, e. G., clinical blood samples, for storage or transportation before use. Exemplary applications include but are not limited to enrichment of leukocyte subtypes such as t-cells or neutrophils for cytokine and immuno-assays; isolation of progenitor cells from cord blood or peripheral blood for transplantation; isolation of fetal cells from the maternal blood for diagnosis; and ...

Methods and compositions are provided for preparing a biological specimen for microscopic analysis. These methods find many uses, for example in medicine and research, e. G., to diagnose or monitor disease or graft transplantation, to study healthy or diseased tissue, to screen candidate agents for toxicity and efficacy in disease modification. Also provided are reagents, devices, kits and systems thereof ...

The present invention provides methods for analyzing blocks of closely spaced snps, or haplotypes for use in identification of the origin of dna in a sample. The methods comprise aligning common alleles of a gene of interest and identifying a region containing a plurality of snps which is flanked by non-polymorphic dna which can be used for primer placement. Any ...

A method, system, and apparatus for treating a patient with hiv. A vector can be modified from a thymidine kinase gene. The modified vector is expressed in the presence of tat rna. The modified vector is then package and delivered to hiv-infected cells. The replication of hiv is inhibited by eliminating infected cells in the presence of ganciclovir. Modified cells ...