|| List of recent Recombinant-related patents
| Drought tolerant plants and related constructs and methods involving genes encoding ferredoxin family proteins|
Isolated polynucleotides and polypeptides and recombinant dna constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant dna constructs, and methods utilizing these recombinant dna constructs. The recombinant dna construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a ferredoxin family protein..
| Compositions and methods for the biosynthesis of vanillan or vanillin beta-d-glucoside|
Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express a mutant arom polypeptide and/or mutant catechol-o-methyltransferase polypeptide alone or in combination with one or more vanillin biosynthetic enzymes or udp-glycosyltransferases (ugts). Such microorganisms, plants, or plant cells can produce vanillin or vanillin beta-d-glucoside..
| Antibodies comprising chimeric constant domains|
Antibodies, antigen-binding proteins and fc-fusion proteins that comprise recombinant polypeptides containing a chimeric heavy chain constant region sequence are provided that bind to certain fc receptors however have reduced effector functions. Methods of making constructs for expression of such chimeric fc-containing antibodies, antigen-binding proteins and fc-fusion proteins in cell systems, and methods of producing and isolating the chimeric fc-containing proteins are provided..
| Pentose fermentation by a recombinant microorganism|
The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.. .
| Bioconversion process for producing nylon-7, nylon-7, 7 and polyesters|
Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional c7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional c7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters..
| Enzymatically catalyzed method of preparing mono-acylated polyols|
The present invention relates to a biocatalytic method of preparing a mono-acylated polyol catalyzed by triacylglycerol lipase mutants, as for example derived from candida antarctica lipase b (calb); a biocatalytic method of enantioselectively preparing an asymmetric mono-acylated polyol, catalyzed by the same enzyme mutants; as well as the use of a mutated triacylglycerol lipase in a method of preparing mono-acylated polyols. The invention also provides novel mutants, coding sequences thereof, and recombinant microorganisms carrying said coding sequences..
| Recominant ralstonia eutropha capable of producing polyactic and acid or polylatic acid polymer, and method for producing polyactic acid or polylatic acid copolymer using the same|
Provided are a recombinant ralstonia eutropha capable of producing polylactate or a hydroxyalkanoate-lactate copolymer, and a method of preparing polylactate or a hydroxyalkanoate-lactate copolymer using the same. The recombinant ralstonia eutropha, which is prepared by introducing a gene of an enzyme converting lactate into lactyl-coa and a gene of a polyhydroxyalkanoate (pha) synthase using lactyl-coa as a substrate thereto, may be cultured, thereby efficiently preparing a lactate polymer and a lactate copolymer..
| Hybrid organic-inorganic system for producing biofuels|
The present invention provides for a system for converting co2 and h2 to one or more biologically derived compounds. In some embodiments, the system comprises a host cell comprising one or more nucleic acids encoding genes for a recombinant surface display protein which is capable of tethering an electrocatalyst molecule, such as a cobalt(ii) complex supported by tetradentate polypyridyl ligand 2-bis(2-pyridyl)(methoxy)methyl-6-pyridylpyridine (py4), and enzymes for synthesizing a biologically derived compound, such as an alkane, alcohol, fatty acid, ester, or isoprenoid..
| Oxidation and amination of primary alcohols|
The present invention relates to a method comprising the steps a) providing a primary alcohol of the formula ho—(ch2)x—r1, wherein r1 is selected from the group consisting of —oh, —sh, —nh2 and —coor2, x is at least 3 and r2 is selected from the group consisting of h, alkyl and aryl, b) oxidizing the primary alcohol by contacting it with an nad(p)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, wherein the nad(p)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a primary alcohol.. .
| Artificial introns|
The invention concerns the field of recombinant gene engineering. It concerns novel artificial introns and compositions comprising such introns as well as a method to improve expression of polypeptides from nucleic acids such as cloned genes, especially genes encoding antibodies and antibody derived fragments, and the production of various polypeptides in eukaryotic host cells using said novel artificial intron sequences..
| Regulatable promoter|
A method of producing a protein of interest (poi) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a poi under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the poi at a transcription rate of at least 15% as compared to the native pgap promoter, and c) producing and recovering the poi; and further an isolated regulatable promoter and a respective expression system.. .
| Method of diagnosing and preventing pneumococcal diseases using pneumococcal neuraminidases|
A method of providing protection against pneumococcal infection in a subject is disclosed. The method includes steps of administering to the subject a composition that includes combination of three recombinant pneumococcal neuraminidases: nana, nanb, and nanc of s.
| Polypeptide having phytase activity and increased temperature resistance of the enzyme activity, and nucleotide sequence coding said polypeptide|
The invention relates to a recombinant dna molecule encoding a polypeptide having phytase activity and increased temperature stability and increased proteolytic stability of the enzyme activity. The dna sequence has been obtained by variation of the mature wild-type e.
| Cdna construct of salmonidae alphavirus|
The invention concerns recombinant dna's comprising cdna of genomic rna of a salmonidae alphavirus preceded by a spacer sequence, under the control of a suitable promoter. Said recombinant dnas are useful for obtaining expression vectors, producing recombinant salmonidae alphavirus, and for obtaining vaccines..
| Composition and formulation comprising recombinant human iduronate-2-sulfatase and preparation method thereof|
Disclosed is a composition comprising recombinant iduronate-2-sulfatase (ids). The glycosylation pattern and formylglycine content of the ids composition are different from those of elaprase and have superior pharmaceutical efficacy and are safer than the conventional agent and thus can be effectively used for the therapy of hunter syndrome..
| St6gal i mediated modulation of hematopoiesis|
Provided are methods for reducing haematocytes in an individual. The method involve administering to an individual a composition that contains recombinant a2,6-sialyltransferase (st6gal i).
|Expression enhancing intron sequences|
The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (ime) of gene expression.
|Gene delivery system and method of use|
A recombinant replication competent retrovirus for gene deliver and gene therapy is provided. The recombinant retrovirus has a heterologous nucleic acid sequence, a sequence encoding a cell- or tissue-specific ligand or a sequence for transcriptional targeting, or a combination of both a cell- or tissue-specific ligand and a cell- or tissue-specific transcriptional targeting sequence..
|Egvii endoglucanase and nucleic acids encoding the same|
The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding egvii amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding egvii, recombinant egvii proteins and methods for producing the same..
|Bgl7 beta-glucosidase and nucleic acids encoding the same|
The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl7, and the corresponding bgl7 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding bgl7, recombinant bgl7 proteins and methods for producing the same..
|Bgl6 beta-glucosidase and nucleic acids encoding the same|
The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl6, and the corresponding bgl6 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding bgl6, recombinant bgl6 proteins and methods for producing the same..
|Recombinant anaerobic acetogenic bacteria for production of isoprene and/or industrial bio-products using synthesis gas|
This invention provides for recombinant anaerobic acetogenic bacterial cells having one or more nucleic acids whereby isoprene, mevalonate and/or other industrial bio-products are produced in a substantially oxygen-free culture condition using synthesis gas as energy and/or carbon source.. .
|Aspergillus fumigatus cellulolytic enzyme compositions and uses thereof|
The present invention relates to recombinant trichoderma host cells producing aspergillus fumigatus cellulolytic enzyme compositions and methods of producing and using the compositions.. .
|Dna polymerases with increased 3'-mismatch discrimination|
Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
|Cell culture improvements|
The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture..
|Methods for producing modified glycoproteins|
Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts.
|Heterologous intron within an immunoglobulin domain|
The invention concerns the field of recombinant gene engineering. It concerns novel introns and compositions comprising such introns as well as a method to improve expression of polypeptides from nucleic acids such as cloned genes with heterologous introns, especially genes encoding antibodies and antibody derived fragments, and the production of various polypeptides in eukaryotic host cells using said novel intron sequences as heterologous introns..
|Coenzyme-linked glucose dehydrogenase and polynucleotide encoding the same|
The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to fad to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor..
|Newcastle disease virus vectored avian vaccines|
The present invention encompasses engineered newcastle disease virus (ndv) vaccines or compositions. The vaccine or composition may be a recombinant vaccine.
|Vaccibodies targeted to cross-presenting dendritic cells|
The present invention relates to recombinant fusion proteins targeted to dendritic cells and uses thereof. In particular, the present invention relates to fusion proteins comprising an antibody component and a targeting components, and uses of such fusion proteins to trigger immune responses..
|Antibody constant domain regions and uses thereof|
The invention provides recombinant protein (e.g., a recombinant antibody or soluble receptor) having (i) a binding domain capable of specific binding to an epitope (for example an antibody variable domains, a receptor, a growth factor, a cytokine, or a fragment of any of the foregoing which is capable of specifically binding the desired epitope) and (ii) an effector domain comprising a constant domain region which is derived from immunoglobulin of a first species which is a companion mammal, e.g. Dog, cat, or horse, having engineered substitutions at one or more positions and having an altered interaction with one or more fcrs or other ligands, and optionally enhanced effector function, relative to the parent constant domain region..
|Composition and methods for the treatment of degenerative retinal conditions|
The present invention is directed to compositions and methods for the treatment of degenerative retinal conditions. According to a general aspect, the present invention is directed to inflammatory mediators, preferably components or substrates of the nlrp3-inflammasome, for use in the treatment of degenerative retinal conditions involving drusen and anaphylatoxin-induced choroidal-neovascularisation.
|Antigen-binding proteins comprising recombinant protein scaffolds|
Disclosed herein are recombinant protein scaffolds and recombinant multifunctional protein scaffolds for use in producing antigen-binding proteins in addition, nucleic acids encoding such recombinant protein scaffolds, recombinant multifunctional protein scaffolds, and antigen-binding proteins are provided vectors and cells useful for expression of the described proteins are also provided, as are methods of use.. .