|| List of recent Reagent-related patents
|Method for isolating nucleic acids from formalin-fixed paraffin embedded tissue samples|
Methods are disclosed for isolating nucleic acids from formalin-fixed paraffin embedded (ffpe) tissue samples. Each of tissue samples contains paraffin and a target biological tissue or material, and the method includes the steps of: adding a first reagent and a second reagent to the ffpe tissue sample, the first reagent dissolving the paraffin material and the second reagent lysing the biological tissue; mixing the first reagent, the second reagent, and the ffpe tissue sample to form a first mixture; (2) heating the first mixture at 50-80° c.
|Protein enriched microvesicles and methods of making and using the same|
Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein.
|Functionalizing reagents and their uses|
Reagents and methods for functionalizing polypeptides with moieties (alkylene glycol) molecules and glycan groups are disclosed that are based on a functionalizing reagent which comprises a nitrogen containing heterocyclic aromatic ring having a vinyl substituent that is capable of reacting with one or more thiol groups that are naturally present, or have been introduced into, the polypeptide, for example by employing a thiol group of one or more cysteine residues. The functionalizing reagent is covalently linked to a poly(alkylene glycol) molecule, such as a polyethylene glycol (peg) molecule, or a glycan group so that the reaction between the vinyl group and the thiol group in the polypeptide covalently links the polypeptide to the poly(alkylene glycol) molecule and/or the glycan group..
|Versatile and stereospecific synthesis of gamma,delta -unsaturated amino acids by wittig reaction|
Compounds of general formula (i) may be useful as therapeutic substances, or as reagents or intermediates for fine chemistry.. .
|Method for avoiding influence of endogenous lipoprotein and reagent|
To identify the aforementioned interference component present in serum or plasma, to thereby provide means for avoiding any interference effect caused by the component.. .
|Methods to accelerate the binding of biological molecules|
A method is provided for enhancing the binding of reagents to ligands. In the method a ligand is immobilized on a porous support and incubated with a reagent solution.
|Compatible solute ectoine as well as derivatives thereof for enzyme stabilization|
Dry reagent compositions are disclosed for maintaining and preserving enzymatic activity. Also disclosed are test elements incorporating the same and methods of making and using the same for determining the presence or amount of an analyte in a body fluid sample.
|Method and device for assaying an antigen present on erythrocytes or an antibody binding to an antigen present on erythrocytes|
An assaying kit is for pre-transfusion blood group matching between two blood samples. The kit includes at least two assemblies (101,201) corresponding to the first and the second blood sample, each assembly has at least two test units (1).
|Sensitive and rapid determination of antimicrobial susceptibility|
The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration.
|Methods for increasing efficacy of folr1 cancer therapy|
Methods to improve the success of cancer therapies that target the human folate receptor 1 are provided. Kits comprising reagent useful in the methods are further provided..
|Process for producing hydrogen or heavy hydrogens, and hydrogenation (protiation, deuteration or tritiation) of organic compounds using same|
An object is to provide a process for providing hydrogen or heavy hydrogens conveniently without the necessity of large-scale equipment and a process capable of performing hydrogenation (protiation, deuteration or tritiation) reaction conveniently without the use of an expensive reagent and a special catalyst. The production process includes a process for producing hydrogen or heavy hydrogens, containing subjecting water or heavy water to mechanochemical reaction in the presence of a catalyst metal, and a process for producing a hydrogenated (protiated, deuterated or tritiated) organic compound, containing subjecting an organic compound and water or heavy water to mechanochemical reaction in the presence of a catalyst metal..
|System and method for sequential injection of reagent to reduce nox from combustion sources|
In a large exhaust duct from a lean burn combustion source, such as a boiler, diesel engine or gas turbine, multiple injectors can be used to inject a reagent, such as an aqueous solution of urea or ammonia, into the exhaust for use in the catalytic reduction of nox in a process known in the art as selective catalytic reduction (scr). When operating at low injection rates, such as during low combustor loads, the injectors are operated individually for short periods of time in a sequential manner..
|Dry eye diagnostic|
The present invention discloses a tear analyzing strip (tas) for measuring dry eye syndrome in a patient. The tas comprises an elongated body comprising a proximal portion and a distal portion interconnected by a main longitudinal axis.
|Preparation method of lycopene intermediate 3-methyl-4,4-dialkoxy-1-butaldehyde|
Disclosed is a preparation method of the lycopene intermediate 3-methyl-4,4-dialkoxy-1-butaldehyde. The preparation method comprises the following steps: (1) reacting 2-methyl-3,3-dialkoxy-1-halopropane with magnesium powder in the solvent of anhydrous tetrahydrofuran at a temperature of 45˜65° c.
|Transvinylation as a first stage of coupling production of vinyl esters and acetic acid or propionic acid reaction products|
Process for coproduction of a vinyl ester of the formula r—c(o)o—ch═ch by transvinylation reaction of a carboxylic acid of the formula r—c(o)oh with a transvinylating reagent of the formula r1—c(o)o ch═ch2, characterized in that (a) the transvinylation reaction is conducted continuously at a temperature of 90 to 160° c. And at a pressure of 0.5 to 15 mpa without withdrawal of a reactant in the presence of a transition meta/catalyst containing at least one transition metal selected from the group of ruthenium, osmium, rhodium, iridium, palladium and platinum; (b) the resulting reaction mixture is separated into its constituents and the vinyl ester of the formula r—c(o)o—ch═ch2 and the carboxylic acid of the formula r1—c(o)—oh are removed; and (c) the carboxylic acid obtained after step (b) is converted to a derivative of the formula r1—c(o)—x, r1—ch2—oh or r6—c(o)—oh in which x is vinyloxy, o—ch—ch2, halogen, alkoxy of the formula or2 in which r2 is a substituted or unsubstituted hydrocarbyl radical having 1 to 10 carbon atoms, amino of the formula nr3r4 in which r3 and r4 are each independently hydrogen or a substituted or unsubstituted hydrocarbyl radical having 1 to 10 carbon atoms, or carboxyl of the formula o—c(o)—r5 in which r5 is hydrogen or a substituted or unsubstituted hydrocarbyl radical having 1 to 10 carbon atoms, and r6 is the partly or fully halogen-substituted r1 radical..
|Gene expression signatures of neoplasm responsiveness to therapy|
Gene signatures for determining whether a neoplasm (such as a multiple myeloma neoplasm) is sensitive to mtori/hdaci combination therapy and/or for determining the prognosis of a neoplasm in a subject are described. Some embodiments include determining whether a neoplasm is sensitive to mtori/hdaci combination therapy by predicting whether mtori/hdaci combination therapy will successfully treat the neoplasm, for example increasing survival of the subject with the neoplasm.
|Functionalized monomers and polymers|
This invention relates to a composition, comprising: an unsaturated functionalized monomer of from about 5 to about 30 carbon atoms, which is: (a) polymerized to form a functionalized polymer; (b) copolymerized with a comonomer to form a functionalized copolymer; or (c) reacted with an enophilic reagent to form a polyfunctionalized monomer. The polyfunctionalized monomer may be polymerized to form a polyfunctionalized polymer which may be further reacted with one or more additional reagents.
|Windowing combined with ion-ion reactions for chemical noise elimination|
In a first location of a mass spectrometer, a plurality of ionized molecules of an ion source are selected that have mass-to-charge ratios within a mass-to-charge ratio window width. The plurality of selected ionized molecules are transmitted from a first to a second location.
|Dispensing unit for dispensing preservation fluid into a tissue sample container|
The invention relates to a dispensing unit for dispensing fluid into an external container. The dispensing unit comprises: a connector, a reagent cylinder for storing the fluid, and a locking ring.
|Information notification sample processing system and methods of biological slide processing|
A sample processing system that may be automated and methods are disclosed where samples are arranged on a carrier element and a process operation control system automatically processes the samples perhaps robotically with an operationally-influential exteriorly-consequential information monitor or a data capture element. Significant process details as well as operationally-influential exteriorly-consequential information may be monitored and an automatic notice element may cause notification of a person at some display that may be remote.
|Hematological analyzer, method for analyzing body fluid and computer program product|
A hematological analyzer for measuring blood, sets a body fluid measurement mode; receives a measurement start instruction; irradiates a measurement sample with light and obtains optical information from cells contained in the measurement sample; and classifies at least white blood cells and nucleated cells other than white blood cells contained in the measurement sample, and counts the white blood cells and nucleated cells other than white blood cells based on the optical information obtained from the cells in the measurement sample prepared from a body fluid sample and white blood cell measuring reagent when the body fluid measurement mode has been set and the measurement start instruction has been selected, is disclosed. A method for analyzing body fluid and a computer program product are also disclosed..
|Bacteria analyzing method and specimen analyzer|
Disclosed is a bacteria analyzing method comprising: irradiating with light a measurement sample prepared by mixing a specimen and a reagent; obtaining two types of optical information from each of at least some particles contained in the measurement sample; and generating a measurement result of the specimen with a flag representing morphological characteristics of bacteria contained in the specimen based on both of: (i) information indicative of a characteristic of a distribution pattern of particles plotted in a first region of a coordinate space including at least two axes, wherein the two types of optical information are scalable along the respective axes, and (ii) information representing a number of particles plotted in a second region being a part of the first region.. .
|Method for measuring beta-glucan, and beta-glucan-binding protein for use in the method|
Disclosed are a method for measuring βg comprising the steps of bringing a sample into contact with a βg-binding protein 1 and a βg-binding protein 2, each comprising an amino acid sequence which is identical or substantially identical to an amino acid sequence shown in any one of seq id no:4, seq id no:6, seq id no:8, seq id no:10, seq id no:14, seq id no:16, seq id no:18, or seq id no:20, and having the β-glucan binding activity, to form a complex of the βg-binding protein 1, βg in the sample and the βg-binding protein 2, measuring quantity of the complex, and determining βg concentration in the sample based on the quantity of the complex; a reagent and a kit for use in said method; a novel βg-binding protein; a nucleic acid molecule encoding the βg-binding protein; and a method for producing the aforementioned βg-binding protein.. .
|Reducing non-specifically bound molecules on supports|
Methods and reagents are disclosed for preparing a support for reaction of the support with an assay molecule. In the method the support is treated with a detergent at a concentration of about 0.01% to about 5% (by weight) at a temperature of about 4° c.
|Method for transcriptional amplification of nucleic acids combining steps of different temperatures|
A method of transcriptional amplification includes: (a) obtaining a mixture by combining (i) a biological sample comprising nucleic acids, (ii) amplification primers, (iii) amplification reagents including enzymes required for amplification, and (iv) at least one polyol capable of stabilizing the enzymes required for amplification; (b) denaturing the nucleic acids by heating the mixture at a temperature above 41° c.; and (c) transcriptionally amplifying at least one target nucleic acid at a temperature above 41° c. When present in the mixture..
|Process for preparing a core-shell structured lithiated manganese oxide|
The invention relates to a process for preparing a core-shell structured lithiated manganese oxide, comprising the steps of providing spinel limxmn2-xo4 particles, where m is one or more metal ions selected from the group consisting of li, mg, cr, al, co, ni, zn, cu, and la, and 0≦x<1, as core particles, and subjecting the spinel particles to a heat-treatment with a reactive chemical reagent in the form of liquid or gas to form a shell layer on the surface of the core particles, and to the prepared core-shell structured lithiated manganese oxide, and its use as a cathode material for a lithium ion battery. .
|Enzymatic conjugation of antibodies|
The present application relates to methods for the functionalization of immunoglobulins, in particular with drugs. Also disclosed herein are linking reagents, functionalized antibodies, pharmaceutical compositions, and method of treating disease and/or conditions..
|Compositions and methods related to tissue targeting|
Systems and reagents for identification, characterization and/or targeting of particular tissue or cell markers are disclosed. Methods and compositions for in vivo and in vitro targeting of particular targets are also disclosed.
|Calligraphy paper-based biomedical testing sheet and its manufacturing method|
A biomedical testing sheet comprises: a substrate with calligraphy paper material, wherein the substrate comprises a wax pattern layer, which is a part of a surface of the substrate coated by a waxy material, and penetrated and diffused by the waxy material, and the wax pattern layer comprises one or more carrying units for carrying one or more droplets of liquids to be measured. The lotus effect substantially prevents diffusion of the liquids and reagents on the carrying units, and reduces the required amount of liquids to be measured and the amount of agent..
|Automatic analysis device and reagent processing method in automatic analysis device|
An automatic analyzer is capable of performing a preparation process on a reagent used to measure a highly urgent test item even during analysis operation, and is configured as follows. If there is no parameter for the analysis item regarding a new reagent, the parameters are downloaded.
|Separation of luminescent nanomaterials|
The invention relates to analysis of photon upconversion luminescent inorganic lanthanide-doped nanomaterials and/or separation and/or purification of them from other materials such as biomolecules and/or chemicals used e.g. For bioconjugation in preparation of reagents based on upconverting lanthanide nanoparticles for bioanalytical assays.
|Method for sensor calibration|
The present invention provides a method of determining the amount of an optical probe species binding to or releasing from an optical sensor surface characterized in that the determination comprises the steps of: a) determining, at one single wavelength or at more than one wavelength, a physical measurand (xi) that is related to the absorptivity of said probe, b) correlating the value of the measurand to the amount of said optical probe species binding to or releasing from said surface, respectively, wherein the physical measurand (xi) of step a) is a physical measurand in which the contribution from the refractive index is substantially zero. The present invention further provides different uses of a peak width as well as a computer program product and reagent kits for the disclosed methods..
|Methods for asymmetric e poxidation using flow reactors|
Embodiments of the present disclosure relate to asymmetric epoxidation of olefinic alcohols, using a chiral alcohol chelated titanium catalyst and an organic peroxide performed in a microreactor flow reactor system that can comprise multiple microreactor modules. Molecular sieves can be used to remove any adventitious water in the reagent feed solutions and ensure an anhydrous reaction solution.
|Rapid fluorescence tagging of glycans and other biomolecules with enhanced ms signals|
Reagents comprising ms active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as n-glycans and uses thereof are taught and described.. .
|Alpha-hydrogen substituted nitroxyls and derivatives thereof as catalysts|
Novel alpha-hydrogen substituted nitroxyl compounds and their corresponding oxidized (oxoammonium cations) and reduced (hydroxylamine) forms, and the use of such compounds, inter alia, for oxidation of primary and secondary alcohols to aldehydes and ketones, respectively; resolution of racemic alcohols; desymmetrization of meso-alcohol; as radicals and spin trapping reagents; and as polymerization agents. Processes for preparing the novel nitroxyl/oxoammonium/hydroxylamine compounds from the corresponding amines, and certain novel amine derivatives and their uses.
|Asymmetric electrophilic fluorination using an anionic chiral phase-transfer catalyst|
The discovery of distinct modes of asymmetric catalysis has the potential to rapidly advance chemists' ability to build enantioenriched molecules. As an example, the use of chiral cation salts as phase-transfer catalysts for anionic reagents has enabled a vast set of enantioselective transformations.
|In situ oligonucleotide synthesis on a paramagnetic support|
A novel method for attaching oligonucleotides to a paramagnetic solid support is disclosed. Conventional methods of attachment require that oligonucleotides be pre-synthesized with specific end modifications, which is laborious and expensive.
|Insect g-coupled receptors useful as targets for insecticides and compounds and reagents identified using the same|
An approach to identify and evaluate potential insecticide targets using publicly available genome (dna) sequence information for arthropod disease vector is provided. The utility of this approach is demonstrated by first determining the molecular and pharmacological properties of two different dopamine (neurotransmitter) receptors identified in the genome of the yellow fever- and dengue-transmitting mosquito, aedes aegypti.
|Enhancement of sensitivity and specificity of ketosteroids and keto or aldehyde containing analytes|
A method, a labeling reagent, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological matrices.
|Methods of producing competitive aptamer fret reagents and assays|
Methods are described for the production and use of fluorescence resonance energy transfer (fret)-based competitive displacement aptamer assay formats. The assay schemes involve fret in which the analyte (target) is quencher (q)-labeled and previously bound by a fluorophore (f)-labeled aptamer such that when unlabeled analyte is added to the system and excited by specific wavelengths of light, the fluorescence intensity of the system changes in proportion to the amount of unlabeled analyte added.
|Reagent card alignment system and method|
A reagent card analyzer comprises an optical signal source configured to transmit an optical signal and an optical signal detector spaced a distance from the optical signal source to define an optical signal path into which the optical signal is transmitted, the optical signal detector configured to detect the optical signal and to output an electrical signal indicative of the optical signal. A reader is configured to read a reagent pad of a reagent card.
|Car+ t cells genetically modified to eliminate expression of t-cell receptor and/or hla|
The present invention concerns methods and compositions for immunotherapy employing a modified t cell comprising disrupted t cell receptor and/or hla and comprising a chimeric antigen receptor. In certain embodiments, the compositions are employed allogeneically as universal reagents for “off-the-shelf treatment of medical conditions such as cancer, autoimmunity, and infection.
|Method of detecting a biological activity|
The present invention provides method of detecting a predetermined biological activity. The method includes using an aqueous mixture comprising a first indicator reagent with a first absorption spectrum and a second indicator reagent.
|Preparation of an aqueous reagent for the absorption or destruction of pollutants|
Process for treating a medium by the removal or destruction of one or more undesired substances present in said medium, comprising combining hydrogen peroxide and alkali hydroxide in an aqueous solution to form superoxide, and bringing the resultant superoxide-containing solution into contact with said medium. The process is useful for the destruction of halogenated organic pollutants and also for carbon dioxide removal from flue gases.
|Method for improving gold recovery|
Disclosed are methods and compositions for improving metal extraction processes by introducing at least one cationically charged compound selected from a group consisting of amines, particularly quaternary amines such as alkyldimethylbenzylammonium chlorides (adbac compounds), polyamines and other suitable cationic organic materials, and mixtures thereof, for neutralizing and/or coagulating excess anionic organic compounds including, for example, flotation reagents, surfactants, polymers, flotation reagent by-products and/or anti-scalant additives found in metal concentrate streams, and thereby reduce the downstream fouling of the activated carbon by these compounds.. .
|Single, multi-walled, functionalized and doped carbon nanotubes and composites thereof|
The present invention relates to single walled and multi-walled carbon nanotubes (cnts), functionalized cnts and carbon nanotube composites with controlled properties, to a method for aerosol synthesis of single walled and multi-walled carbon nanotubes, functionalized cnts and carbon nanotube composites with controlled properties from pre-made catalyst particles and a carbon source in the presence of reagents and additives, to functional, matrix and composite materials composed thereof and structures and devices fabricated from the same in continuous or batch cnt reactors. The present invention allows all or part of the processes of synthesis of cnts, their purification, doping, functionalization, coating, mixing and deposition to be combined in one continuous procedure and in which the catalyst synthesis, the cnt synthesis, and their functionalization, doping, coating, mixing and deposition can be separately controlled..
|Mixing apparatus and methods|
A method and apparatus for the mixing of a solution and reagents for various reactions and/or testing having a closed cartridge reaction well, a magnetically responsive bead within the well having a chemically inert coating. A heat source then heats the contents to a target temperature while oscillating magnetic fields move the bead within the well in order to mix the contents and make the contents of the reaction well homogeneous..
|Dry flue gas desulfurization system with dual feed atomizer liquid distributor|
An atomizer system 160 for use in a spray dry absorber 130, the atomizer system 160 includes a support member 161, an atomizing disc 162 rotatably coupled to the support member 161, a first feed line 163 for delivery of a reagent slurry 163a to a delivery area 165 and a second feed line 164 for delivery of a dilution liquid 164a to the delivery area 165. The delivery area 165 is positioned within the atomizer system 160 to reduce scale buildup generated by interaction of the reagent slurry 163a and the dilution liquid 164a..
|Dripless, permanent sealing assembly for container|
An embodiment provides a permanent sealing assembly for a container, such as a reagent bottle. The permanent sealing assembly allows for drip-less reagent container exchange for liquid analysis instruments.
|Method for producing vinyl esters|
The present invention relates to a continuous, catalytic process for preparing a vinyl ester of the formula r—c(o)o—ch═ch2 by reaction of a carboxylic acid of the formula r—c(o)oh with a transvinylating reagent of the formula r1—c(o)o—ch═ch2, wherein the reaction is effected at a temperature of 90 to 160° c. And at a pressure of 0.5 to 15 mpa without withdrawal of a reactant in the presence of a transition metal catalyst containing at least one transition metal from the group of ruthenium, osmium, rhodium, iridium, palladium and platinum, and then the resulting reaction mixture is separated into its constituents..
|Process for the synthesis of 4,5,6,7-tetrachloro-3',6'-dihydroxy-2',4',5',7'-tetraiodo-3h-spiro[isobenzofuran-1,9'-xanthen]-3-one (rose bengal) and related xanthenes|
A new process for the manufacture of iodinated xanthenes in high purity includes a cyclization step followed by an iodination step. No extraction, chromatographic or solvent concentration steps are required, and the intermediate as well as final compounds are isolated via filtration or similar means.
|Ultraviolet cured optic binder|
The ultraviolet cured optic binder has low curing shrinkage, low hardness and high optical transmittance. Technology programs are as follows: an ultraviolet cured optic binder composition includes the following raw material in parts by weight: 50 to 100 parts of prepolymer, 20 to 90 parts of inert resin, 35 to 70 parts of monomer, 5 to 12 parts of photoinitiator, 2 to 3 parts of coupling reagent, 1 to 2 parts of auxiliaries..
|Process for direct conversion of biomass to liquid fuels and chemicals|
A method of catalytically preparing a fluid product from solid carbonaceous material is described. In the method, at least one of the following equilibria is established by one or more catalysts: a) ch3oh═co+2h2, b) co+h2o═co2+h2.
|Method for measuring physiologically active substance of biological origin|
A method for measuring a specific physiologically active substance of biological origin includes preparing a mixed solution of an al reagent and a sample containing the physiologically active substance and detecting the aggregation or gelatinization of a protein in the mixed solution while agitating the mixed solution, thereby detecting the physiologically active substance or measuring the concentration of the physiologically active substance in the sample. A predetermined fluorescence emission is imparted to a substance involved in the aggregation or gelatinization in the sample to be measured and/or the al reagent, and the fluorescence emitted from the substance involved in the aggregation or gelatinization is measured or observed in the mixed solution.
|Measurement of lactic acid in biological fluids|
We propose a procedure for the determination of lactic acid or lactate in biological fluids, which is simple and low cost with respect to the known methodologies. This procedure can be used by unskilled personnel and it can be implemented in disposable test strips and portable measure devices.
|Water chemistry test kit apparatus and related methods|
Methods and apparatus are disclosed for an improved water chemistry test. Features disclosed include, without limitation, a test block, colored liquid reagents, and color comparison displays, with test chambers/vials that are generally horizontal.
|Islet cells from human embryonic stem cells|
This disclosure provides a system for producing pancreatic islet cells from embryonic stem cells. Differentiation is initiated towards endoderm cells, and focused using reagents that promote emergence of islet precursors and mature insulin-secreting cells.
|Photo-bioreactor (algae greenhouse-dry cooling tower)|
This closed system photo-bioreactor (pbr) system includes all of the necessary equipment to naturally collect solar energy and through photosynthetic process produces useable algal biomass. This pbr utilizes waste heat and waste co2 exhaust from power plants or other industrial sources plus waste or other nutrients from domestic, agriculture or other systems.
|Method and composition for a protein transduction technology and its applications|
A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier.
|Reagent for labeling primary amine groups in proteins|
A reagent includes a magnetic nanoparticle, a cleavable linker, and a primary amine reactive group. A first end of the cleavable linker may conjugate to a surface of the magnetic nanoparticle.
|Method for measuring cells, and reagent for cell measurement|
A method and a means are provided, by which multiple types of cells can be simultaneously measured with high sensitivity by an atp luminescence method. A method for measuring cells in a sample is provided, which comprises the steps of adding methanol to a sample suspected of containing viable cells to increase atp within viable cells, extracting intracellular atp, and causing extracted atp to emit luminescence..
|Biological sample treatment apparatus|
Apparatus for treating biological samples disposed on substrates, including: an input buffer for receiving one or more substrate holders each being adapted to support a plurality of the substrates; a treatment zone including a plurality of treatment stations each being adapted to receive one of the substrates; a reagent dispenser configured by a controller to dispense reagents to the substrates at the treatment stations; a substrate transport device configured by the controller to transport individual substrates between the substrate holders in the input buffer and the treatment stations.. .
|Reagents, methods and kits for classification of fungi and direction of anti-fungal therapy|
Provided herein are methods, kits and compositions to classify fungi. Methods are provided for classification of fungi according to established phenotypes, for example, antimicrobial susceptibility profiles.
|Reagents, methods, and libraries for bead-based sequencing|
The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage.
|Enzymatic synthesis of poly(amine-co-esters) and methods of use thereof for gene delivery|
Poly(amine-co-ester) polymers, methods of forming active agent-load nanoparticles therefrom, and methods of using the nanoparticles for drug delivery are disclosed. The nanoparticles can be coated with an agent that reduces surface charge, an agent that increases cell-specific targeting, or a combination thereof.
|Conjugation of biomolecules using diels-alder cycloaddition|
A method is provided for covalently linking carbohydrates, proteins, nucleic acids, and other biomolecules under neutral conditions, using a diels-alder cycloaddition reaction. In an example, activated carbon-carbon double bonds were attached to free amino sites of a carrier protein, and a conjugated diene was attached to a carbohydrate hapten.
|Method for producing size selected particles|
The invention provides a system for preparing specific sized particles, the system comprising a continuous stir tank reactor adapted to receive reactants; a centrifugal dispenser positioned downstream from the reactor and in fluid communication with the reactor; a particle separator positioned downstream of the dispenser; and a solution stream return conduit positioned between the separator and the reactor. Also provided is a method for preparing specific sized particles, the method comprising introducing reagent into a continuous stir reaction tank and allowing the reagents to react to produce product liquor containing particles; contacting the liquor particles with a centrifugal force for a time sufficient to generate particles of a predetermined size and morphology; and returning unused reagents and particles of a non-predetermined size to the tank..
|Stirring device and automatic analysis apparatus|
According to one embodiment, an automatic analysis apparatus comprises a stirrer, a moving unit, a vibrating unit, and a control unit. The stirrer is configured to stir a solution mixture which includes a sample of a specimen and a reagent corresponding to a measurement item of the specimen, and is stored in a reaction cuvette.
|Mechanically actuated vacuum controlled fluid collection|
A mechanically-actuated vacuum-controlled fluid collection system includes a mechanically-actuated vacuum controller (mavc) to draw fluid into a chamber through the opening to the chamber. The system may include a releasable seal to seal the opening, and the mavc may include a spring-loaded plunger to create a vacuum within the chamber when sealed.
|Method for separating arsenic and heavy metals in an acidic washing solution|
A method for separating arsenic and heavy metals in an acidic washing solution which contains both arsenic and heavy metal, more particularly in a washing solution which is formed in copper smelting and contains sulphuric acid, comprises a separation process section, in which arsenic and at least one primary heavy metal are separated from one another. The separation process section comprises a processing step, in which hydrogen peroxide h2o2 is added to the washing solution, and the separation process section comprises a precipitation stage, in which the washing solution is admixed with a sulphide precipitation reagent, causing the at least one primary heavy metal to precipitate in the form of a metal sulphide.