|| List of recent Polynucleotide-related patents
|Methods and compositions for targeted integration in a plant|
Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed.
|Compositions and methods for modulating myeloid derived suppressor cells|
Provided are methods and compositions for modulating the differentiation of a myeloid derived suppressor cell (mdsc). In particular, described herein are mir-142 polynucleotides and mir-223 polynucleotides that can be used to modulate differentiation of mdscs.
|Method for pairwise sequencing of target polynucleotides|
The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template..
|Subtilase variants and polynucleotides encoding same|
The present invention relates to protease variants and methods for obtaining protease variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants..
|Desaturases and process for the production of polyunsaturated fatty acids in transgenic organisms|
The present invention relates to polynucleotides from cochliobolus heterostrophus c5, cyanothece sp. Ccy0110, mycocentrospora acerin and hyaloperonospora parasitica, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids.
|Fusion proteins comprising type-ii cohesin modules, multi-enzyme complexes comprising same and uses thereof|
Fusion proteins including a type-ii cohesin module that are capable of integrating into native and designer cellulosomes. β-glucosidases modified to include a type-ii cohesin module and polynucleotides encoding same.
|Polypeptides having catalase activity and polynucleotides encoding same|
Provided are isolated polypeptides having catalase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Polypeptides having xylanase activity and polynucleotides encoding same|
The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Genetically engineered herbicide resistant algae|
Algae transformed with one or more polynucleotides encoding proteins that confer herbicide resistance are provided. The algae can be grown in small or large scale cultures that include one or more herbicides for the production and isolation of various products..
The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a recd helicase.
|Novel use of ec-sod for treating angiogenesis-mediated eye diseases|
A method for treating an angiogenesis-mediated eye disease, includes administering to a subject in need thereof an effective amount of ec-sod protein, or a vector having a polynucleotide encoding the ec-sod protein, to treat angiogenesis-mediated eye diseases.. .
|Novel use of ec-sod and method for preparing thereof|
A method for treating a disease, particularly asthma and anaphylactic shock, comprises administering to a subject in need thereof an effective amount of an ec-sod protein or a vector having a polynucleotide encoding thereof.. .
|Polypeptides having beta-glucosidase activity and polynucleotides encoding same|
Provided are isolated polypeptides having beta-glucosidase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Regulatory polynucleotides and uses thereof|
The present disclosure provides compositions and methods for regulating expression of transcribable polynucleotides in plant cells, plant tissues, and plants. Compositions include regulatory polynucleotide molecules capable of providing expression in plant tissues and plants.
|Exonuclease resistant polynucleotide and related duplex polynucleotides, constructs, compositions, methods and systems|
Provided herein are exonuclease resistant polynucleotides and related constructs, compositions, methods and systems.. .
|Self delivering bio-labile phosphate protected pro-oligos for oligonucleotide based therapeutics and mediating rna interference|
Disclosed herein are compositions and methods for generating ribo-nucleic neutral (rnn) or deoxyribo-nucleic-neutral (dnn) polynucleotides with reduced anionic charge, for improved intracellular delivery. Also disclosed herein are methods of using rnn and dnn compositions..
|Methods for indexing samples and sequencing multiple polynucleotide templates|
The invention relates to methods for indexing samples during the sequencing of polynucleotide templates, resulting in the attachment of tags specific to the source of each nucleic acid sample such that after a sequencing run, both the source and sequence of each polynucleotide can be determined. Thus, the present invention pertains to analysis of complex genomes (e.g., human genomes), as well as multiplexing less complex genomes, such as those of bacteria, viruses, mitochondria, and the like..
|Polypeptides having cellobiohydrolase activity and polynucleotides encoding same|
The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Chimeric polypeptides having beta-glucosidase activity and polynucleotides encoding same|
The present invention relates to chimeric polypeptides having beta-glucosidase activity. The present invention also relates to polynucleotides encoding the chimeric polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the chimeric polypeptides.
The invention provides calcium-binding photoproteins which can detect light emission with a higher sensitivity. The proteins of the invention comprising the amino acid sequence of seq id no: 2 can be used for the detection and measurement of calcium ions.
|Methods, compositions and kits for a one-step dna cloning system|
Methods and kits for joining two or more polynucleotides to form a product polynucleotide are provided. A mixture contains a first polynucleotide comprising a selectable marker.
|Chimeric receptors with 4-1bb stimulatory signaling domain|
The present invention relates to a chimeric receptor capable of signaling both a primary and a co-stimulatory pathway, thus allowing activation of the co-stimulatory pathway without binding to the natural ligand. The cytoplasmic domain of the receptor contains a portion of the 4-1bb signaling domain.
|Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement|
Recombinant polynucleotides and recombinant polypeptides useful for improvement of plants are provided. The disclosed recombinant polynucleotides and recombinant polypeptides find use in production of transgenic plants to produce plants having improved properties..
|Polypeptides having lysozyme activity and polynucleotides encoding same|
The present invention relates to isolated polypeptides having lysozyme activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Recombinant adeno-associated virus delivery of alpha-sarcoglycan polynucleotides|
The present invention relates to recombinant adeno-associated virus (raav) delivery of an alpha-sarcoglycan gene. The invention provides raav products and methods of using the raav in the treatment of limb girdle muscular dystrophies such as lgmd2d..
|Polynucleotide capture materials, and methods of using same|
Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as rna and/or dna from such samples. The rna and/or dna is captured by polyamindoamine (pamam (generation 0)) bound to a surface, such as the surface of magnetic particles.
|Sequences and their use for detection and characterization of stec bacteria|
This invention relates to a rapid method for detection and characterization of stec bacteria based on the presence of nucleic acid sequences, in particular, to a pcr-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect stec bacteria in a food or water sample, such as a beef enrichment.
|Microfluidic cartridge and method of making same|
The present technology provides for a microfluidic substrate configured to carry out pcr on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge.
|Chemically modified ligase cofactors, donors and acceptors|
Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates.
|Polypeptides having peroxygenase activity and polynucleotides encoding same|
The present invention relates to isolated polypeptides having peroxygenaseactivity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Biocatalysts for the preparation of hydroxy substituted carbamates|
The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.. .
|Polypeptides having peroxygenase activity and polynucleotides encoding same|
The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Antibodies that bind colony stimulating factor 1 receptor (csf1r)|
Antibodies that bind csf1r are provided. Antibody heavy chains and light chains that are capable of forming antibodies that bind csf1r are also provided.
|Polypeptides having endoglucanase activity and polynucleotides encoding same|
The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides..
|Botulinum neurotoxin e receptors and uses thereof|
An isolated polypeptide comprising an amino acid sequence selected from amino acids 506-582 of sv2a, wherein position 573 is n and is glycosylated, or amino acids 449-525 of sv2b, wherein position 516 is n and is glycosylated. The present invention also provides an antibody that binds specifically to the polypeptide, an isolated nucleic acid comprising a polynucleotide that encodes the polypeptide; a method for reducing bont/e toxicity in an animal; a method for identifying an agent that blocks or inhibits binding between bont/e and an sv2a or sv2b protein; a method for monitoring synaptic vesicle endo- or exocytosis, a method for specifically delivering a chemical entity to a cell which has a specific receptor to a bont toxin.
|Alpha-aminoamidine polymers and uses thereof|
α-aminoamidine polymers and methods of preparing a-aminoamidine polymers by reacting by reacting one or more amines with one or more isocyanides and one or more aldehydes are described. Methods of preparing a-aminoamidine polymers from commercially available starting materials are also provided, wherein the starting materials are racemic or stereochemically pure.
|Second generation virus-like particles (vlp) from epstein-barr viruses for vaccination purposes|
The present invention relates to an epstein-barr virus-like particle (eb-vlp) substantially free of epstein-barr virus (ebv) dna. The present invention also relates to a polynucleotide comprising an ebv genome a) lacking at least one expressible gene selected from the group consisting of the bflf1 gene, the bbrf gene, the bgrf1 gene, the bdrf1 gene, the balf3 gene, the bfrf1a gene, and the bfrf1 gene, and b) producing the eb-vlp of the invention in a suitable host cell.
|Treatment and prevention of viral infections|
Disclosed is polynucleotide encoding a polypeptide comprising an antibody binding site, the polypeptide being able to bind to hcv e2 samples representative of each of hcv genotypes 1-6, as well as polypeptides having such properties and uses of such polypeptides in detecting and treating hcv infection.. .
|Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same|
The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Polynucleotides, polypeptides and methods for enhancing photossimilation in plants|
The present invention relates generally to the field of molecular biology and regards various polynucleotides, polypeptides and methods that may be employed to enhance yield in transgenic plants. Specifically the transgenic plants may exhibit increased yield, increased biomass or increased photoassimilation..
|Isolated polynucleotides and polypeptides, transgenic plants comprising same and uses thereof in improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of plants|
Methods of improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of a plant are provided. According to an aspect the method comprising expressing within the plant an exogenous polynucleotide having a nucleic acid sequence at least 90% identical to seq id nos: 103, 101-102, 104-216, 223-227, 264-416, wherein the nucleic acid sequence is capable of regulating abiotic stress tolerance of the plant, thereby improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of the plant.
|Methods, systems, and computer readable media for improving base calling accuracy|
A method includes exposing template polynucleotide strands, sequencing primers, and polymerase to flows of nucleotide species; obtaining a series of measured intensity values and randomly selecting a training subset therefrom; generating series of base calls using a base caller and aligning the series of base calls to a reference genome or sequence using an aligner; determining intensity value thresholds and parameters of a linear transformation corresponding to different homopolymer lengths and nucleotide species; generating series of base calls corresponding to the series of measured intensity values using at least some of the parameters of a linear transformation; and recalibrating the series of base calls corresponding to the plurality of series of measured intensity values using at least some of the intensity value thresholds.. .
|Cholecystokinin b receptor (cckbr) mini-promoters|
Isolated polynucleotides comprising a cckbr mini-promoters are provided. The mini-promoter may be operably linked to an expressible sequence, e.g.
|High mast2-affinity polypeptides and uses thereof|
The invention relates to polypeptides containing a cytoplasmic domain ending with a mast-2 binding domain, from 11 to 13 residues, the first two residues of which are s and w, and the last four residues of which are q, t, r and l, said polypeptides presenting a high affinity for the pdz domain of the human mast2 protein. The invention also relates to polynucleotides, vectors, lentiviral particles, cells as well as compositions comprising the same.
|Scalable characterization of nucleic acids by parallel sequencing|
A method for detecting the presence or absence of a target polynucleotide in a sample is described.. .
|Engineered listeria and methods of use thereof|
The invention provides a bacterium containing a polynucleotide comprising a nucleic acid encoding a heterologous antigen, as well as fusion protein partners. Also provided are vectors for mediating site-specific recombination and vectors comprising removable antibiotic resistance genes..
|Methods for production of archeae protease in yeast|
The invention relates to: a method for producing a protease in yeast, comprising providing a yeast host cell comprising at least one polynucleotide expression construct encoding a first polypeptide secreted by the host cell in translational fusion with a second polypeptide, wherein the second polypeptide is a protease having a mature amino acid sequence similar to that of seq id no:2; the corresponding yeast host cell and the expression construct.. .
|Method for high-throughput screening of transgenic plants|
The invention relates to a method for quantifying levels of expression and/or quantifying copy number of a heterologous polynucleotide in a transgenic plant using quantitative or real-time polymerase chain reaction (qpcr or real-time pcr), wherein the real-time pcr is performed using a primer set specific to a heterologous terminator sequence operably linked to the heterologous polynucleotide.. .
Methods and reagents for detection and analysis of nucleic acids are provided. Certain methods involves an encoding amplification in which a target sequence is associated with probe-binding sequences and optionally with indexing sequences, (2) an optional distribution step in which the product of the encoding amplification is split into multiple aliquots, and (3) a decoding and detection step in which the presence, absence, quantity, or relative amount of the target sequence in the aliquots is determined.
|Ipnv-isav bivalent vaccine using a virus-like particle-based platform and methods of using the same|
The present invention provides bivalent oral vaccines against infectious pancreatic necrosis virus (ipnv) and infectious salmon anemia virus (isav). Yeast cells comprise an expression vector comprising (i) a polynucleotide sequence encoding a vp2 capsid protein of ipnv and (ii) a polynucleotide sequence encoding one or more antigenic epitopes of hemaglutinin of isav.
|Engineered phenylalanine ammonia lyase polypeptides|
The present invention provides engineered phenylalanine ammonia-lyase (pal) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (pal) polypeptides.. .
|Novel compositions and methods|
The present invention is directed to a polypeptide which comprises: (i) an rv2386c protein sequence; (ii) a variant of an rv2386c protein sequence; of (iii) an immunogenic fragment of an rv2386c protein sequence. In other aspects the invention is directed to associated polynucleotides, fusion proteins and methods for the treatment or prevention of tuberculosis..
|Stress tolerant wheat plants|
The present invention relates to stress tolerant transgenic wheat plants. In particular, the present invention relates to stress tolerant transgenic wheat plants comprising an exogenous polynucleotide encoding a nac transcription factor operably linked to a promoter capable of directing expression of the polynucleotide in a cell of the plant..
|Combination anti-hiv vectors, targeting vectors, and methods of use|
Recombinant lentiviral vectors containing at least: a lentiviral backbone comprising essential lentiviral sequences for integration into a target cell genome; a nucleic acid encoding a ccr5 rnai; and an expression control element that regulates expression of the nucleic acid encoding the ccr5 rnai element, are provided by this invention. In an alternative aspect, the vector also contains polynucleotides encoding trim5 alpha and hiv tar decoy sequences along with gene expression regulation elements such as promoters operatively linked to the polynucleotides.
|Pharmaceutical composition comprising a mixture of carboxylated oligonucleotides|
The invention describes a pharmaceutical composition, comprising a mixture of carboxylated oligonucleotides, obtained by hydrolysis of natural polynucleotides, that results in oligonucleotide mixture, and carboxylation of purine nucleotide bases in the oligonucleotide mixture. The oligonucleotide mixture (dna, rna, or a combination of the two) of plant, animal, or fungal origin is conducted through chemical or biochemical enzymatic procedures, and then, a chemical modification of the oligonucleotides is provided, with carboxylation of their purine nucleotide bases through alkylation with monochloroacetic acid, or acylation with succinic anhydride.
|Fibronectin based scaffold domain proteins that bind to myostatin|
The present invention relates to fibronectin-based scaffold domain proteins that bind to myostatin. The invention also relates to the use of these proteins in therapeutic applications to treat muscular dystrophy, cachexia, sarcopenia, osteoarthritis, osteoporosis, diabetes, obesity, copd, chronic kidney disease, heart failure, myocardial infarction, and fibrosis.
|High throughput method for assembly and cloning polynucleotides comprising highly similar polynucleotidic modules|
The present invention relates to a method for the assembly and cloning of polynucleotides comprising highly similar polynucleotidic modules, that is highly versatile, does not require intermediate amplification step and can be easily automated for high throughput production of customized polynucleotidic modules.. .
|Centroid markers for image analysis of high density clusters in complex polynucleotide sequencing|
Improved compositions, methods, apparatus, and kits for high-throughput nucleic acid amplification, detection and sequencing are disclosed. A nucleic acid cluster having an identifiable center is produced by generating on a solid support an immobilized nucleic acid complement from a template, one of which comprises a detectable label; and amplifying the complement and the template to obtain a nucleic acid cluster on the support, the cluster having a substantially central location marked by the detectable label and a surrounding region comprising immobilized copies.
|Methods and devices for high fidelity polynucleotide synthesis|
Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products..
|Ketoreductase-mediated stereoselective route to alpha chloroalcohols|
The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of α-chloroalcohols from α-chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.
|Polypeptides having glucoamylase activity and polynucleotides encoding same|
The present invention provides isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. The invention also provides nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains..
|Decreased polysaccharide o-acetylation|
The disclosure relates to polypeptides and polynucleotides from multiple species related to the o-acetylation of polysaccharides in plants. The disclosure also describes plants with reduced polysaccharide o-acetylation, methods related to the generation of plants with reduced polysaccharide o-acetylation, polysaccharides with reduced o-acetylation, and methods of using plants and polysaccharides having reduced o-acetylation..
|Polynucleotide and use thereof|
Provided is a polynucleotide including, from the 3′ terminus of the polynucleotide to the 5′ terminus of the polynucleotide, a first region including a nucleotide sequence complementary to a nucleotide sequence of a portion of a target nucleic acid; a second region including a nucleotide sequence identical to a nucleotide sequence of a portion of the target nucleic acid; and a third region including a nucleotide sequence that self-hybridizes to form a stem-loop structure, and compositions, kits, and methods related thereto.. .
|Recombinant fusion interferon for animals|
A recombinant fusion interferon for animals. The recombinant fusion interferon comprises an animal interferon and a fc region of an animal immunoglobulin g (igg).
|Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same|
The present invention relates to isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Hydrogenase polypeptide and methods of use|
Provided herein are polypeptides having hydrogenase activity. The polypeptide may be multimeric, and may have hydrogenase activity of at least 0.05 micromoles h2 produced min−1 mg protein−1.
|Neurotoxins exhibiting shortened biological activity|
The present invention relates to the pharmaceutical field. Specifically, it contemplates a polynucleotide encoding a neurotoxin polypeptide exhibiting a reduced duration of the biological effect in a subject, wherein said polypeptide comprises at least one e3 ligase recognition motif in the light chain, wherein said e3 ligase recognition motif is preferably a binding motif for the e3 ligase mdm2.
|Modified neurotoxins with poly-glycine and uses thereof|
The present invention is concerned with modified neurotoxins. Specifically, it relates to a modified biologically active neurotoxin polypeptide comprising at least one poly-glycine domain.
|Mutant protease biosensors with enhanced detection characteristics|
A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the c-terminal portion of the thermostable luciferase to the n-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase.
|Polypeptides having endoglucanase activity and polynucleotides encoding same|
Provided are isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Micro-utrophin polypeptides and methods|
Described herein are polypeptides, polynucleotides and methods involving a μ-utrophin region or an anti-dystrophinopathic fragment thereof operationally linked to a second region effective to transduce the fusion protein into mammalian muscle cells.. .
|Nucleic acid assembly system|
The present invention relates to a method for the preparation of a library of host cells, a plurality of which comprise an assembled polynucleotide at a target locus, which method comprises: (a) providing a plurality of polynucleotides comprising two or more polynucleotide subgroups, wherein: (i) a plurality of polynucleotides in each polynucleotide subgroup comprises sequence encoding a peptide or polypeptide and/or a regulatory sequence; (ii) a plurality of peptides or polypeptides encoded by, or a plurality of regulatory sequences comprised within, each polynucleotide subgroup share an activity and/or function; (iii) at least one polynucleotide subgroup comprises at least two non-identical polynucleotide species; (iv) a plurality of polynucleotides of each polynucleotide subgroup comprises sequence enabling homologous recombination with a plurality of polynucleotides from one or more other polynucleotide subgroups; and (v) a plurality of polynucleotides in two polynucleotide subgroups comprise a nucleotide sequence enabling homologous recombination with a target locus in host cells; and (b) assembling the plurality of polynucleotides at the target locus by homologous recombination in vivo in host cells, thereby to generate a library of host cells, a plurality of which comprise an assembled polynucleotide at the target locus. The assembled polynucleotides may be recovered, thereby to prepare a library of nucleic acids..
|Systems and methods for determining genetic data|
Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide..
|Colorectal cancer associated circulating nucleic acid biomarkers|
The invention provides methods and reagents for diagnosing colorectal cancer that are based on the detection of biomarkers in the circulating nucleic acids from a patient to be evaluated. In some embodiments, the cna biomarkers are polynucleotide fragments, e.g., dna fragments, that are present at an elevated level in blood, e.g., in a serum or plasma sample, of a colorectal cancer patient in comparison to the level in blood, e.g., a serum or plasma sample, obtained from a normal individual who does not have colorectal cancer..