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Polynucleotide patents

      

This page is updated frequently with new Polynucleotide-related patent applications.




 Sequencing methods patent thumbnailSequencing methods
Described is a method for sequencing a polynucleotide strand by sequencing by synthesis, the method including selecting a predetermined order of nucleotides to provide to a sequencing reaction, the order being selected to correlate with a predicted sequence for the polynucleotide strand; monitoring the reaction to detect incorporation of a nucleotide into a synthesised polynucleotide strand; wherein, in the event that nucleotide incorporation is detected, proceeding to provide the next nucleotide in the predetermined order. In the event that nucleotide incorporation is not detected, the predicted sequence for the polynucleotide strand may be revised and a new predetermined order of nucleotides selected, wherein the new predetermined order is selected to correlate with the revised predicted sequence.
Dnae Group Holdings Limited


 Phase-preotecting reagent flow ordering for use in sequencing-by-synthesis patent thumbnailPhase-preotecting reagent flow ordering for use in sequencing-by-synthesis
A system and machine readable medium for nucleic acid sequencing includes disposing template polynucleotide strands in defined spaces disposed on a sensor array, at least some of the template polynucleotide strands having a sequencing primer and a polymerase operably bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed according to a predetermined ordering; and determining, for each of the series of flows of nucleotide species, how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands, wherein the predetermined ordering (a) is not a series of consecutive repetitions of a 4-flow permutation of four different nucleotide species, (b) is not specifically tailored to a particular combination of a particular template polynucleotide strand to be sequenced and a particular sequencing primer to be used, and (c) comprises a phase-protecting flow ordering.. .
Life Technologies Corporation


 Novel vertebrate cells and methods for recombinantly expressing a polypeptide of interest patent thumbnailNovel vertebrate cells and methods for recombinantly expressing a polypeptide of interest
The present application pertains inter alia to an isolated vertebrate cell suitable for recombinant expression of a polypeptide of interest, wherein the vertebrate cell is altered to impair the function of the endogenous protease matriptase and wherein said cell comprises at least one heterologous polynucleotide encoding a polypeptide of interest and wherein the polypeptide of interest is secreted by the cell. It was found that using respective vertebrate cells for producing a recombinant polypeptide of interest significantly reduces clipping of the polypeptide of interest that is secreted into the cell culture medium.
Novartis Ag


 Nonribosomal peptide synthetases patent thumbnailNonribosomal peptide synthetases
The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (nrps) derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the nrps-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell.
Regents Of The University Of Michigan


 Polynucleotide encoding nf-yb derived from jatropha and use thereof patent thumbnailPolynucleotide encoding nf-yb derived from jatropha and use thereof
By analyzing a jatropha genome, nf-yb-encoding genes of seq id nos: 1 to 11, fragments of nf-yb-encoding genes of seq id nos: 12 and 13, and genes relating thereto were found. By transforming jatropha with these nf-yb-encoding genes and the like, it is possible to overexpress a nf-yb polypeptide and so on, and to significantly improve the productivity of protein synthesis involved by the nf-yb polypeptide, and to significantly improve the dry stress resistance, for example.
Osaka University


 Pullulanase variants and polynucleotides encoding same patent thumbnailPullulanase variants and polynucleotides encoding same
The present invention relates to pullulanase variants having increased thermoactivity and comprising substitutions of the parent pullulanase at one or more positions corresponding to positions 393, 143, 150, 243, 244, 345, 346, 368, 370, 373, 381, 382, 385, 387, 402, 429, 430, 431, 432, 456, 486, 492, 610, 624, 631, 632, 665 and 699 of the polypeptide of seq id no: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Novozymes A/s


 Compositions and methods of engineered crispr-cas9 systems using split-nexus cas9-associated polynucleotides patent thumbnailCompositions and methods of engineered crispr-cas9 systems using split-nexus cas9-associated polynucleotides
The present specification discloses engineered type ii crispr-cas9 systems comprising split-nexus cas9-associated polynucleotides (sn-caspns), including systems comprising three split-nexus cas9-associated polynucleotides (sn1-caspn/sn2-caspn/sn3-caspn) and systems comprising two split-nexus cas9-associated polynucleotides (sn1-caspn/5n2-caspn). Together with a cas9 protein, the sn-caspns facilitate site-specific modifications, including cleavage and mutagenesis, of a target polynucleotide in vitro and in vivo.
Caribou Biosciences, Inc.


 Humanized antibodies against ceacam1 patent thumbnailHumanized antibodies against ceacam1
Humanized antibodies, capable of specific binding to human ceacam1 molecules containing human-to-murine back-mutations in non-cdr variable regions, and their encoding polynucleotide sequences are provided. Pharmaceutical compositions comprising these antibodies as well as methods of their use in treating and diagnosing cancer and other conditions are also provided..
Ccam Biotherapeutics Ltd.


 Fusion proteins, polynucleotides, expression vectors, and their uses patent thumbnailFusion proteins, polynucleotides, expression vectors, and their uses
Provided are fusion proteins, polynucleotides and expression vectors with therapeutic utility in the prevention and/or treatment of cancer, as well as medical uses of these agents, and methods of treatment in which they are used. A fusion protein of the invention comprises an amino acid sequence that provides the apoptosis-inducing activity of trail, and a membrane-anchoring amino acid sequence.
The University Of Liverpool


 Transcription factor genes and proteins from helianthus annuus, and transgenic plants including the same patent thumbnailTranscription factor genes and proteins from helianthus annuus, and transgenic plants including the same
A polynucleotide having at least 80% sequence identity with the fill-length nucleotide sequence of seq id no: 1 and substantially identical polynucleotides; an isolated polypeptide having at least 80% sequence identity with the full-length amino acid sequence of seq id no: 2 and substantially identical polypeptides; and polynucleotides encoding the ha wrky76 polypeptide and substantially identical polypeptides are described. Also described are vectors and recombinant expression cassettes containing the c dna polynucleotide, a polynucleotide encoding the ha wrky76 polypeptide, or substantially identical polynucleotides.
Plant Bioscience Limited


Pancreatic islets of transgenic lea29y animals for treating diabetes

The present invention relates to methods of treating diabetes in a human subject comprising the use of pancreatic islets or of embryonic pancreatic tissue of a transgenic animal, wherein said transgenic animal contains a polynucleotide sequence encoding a ctla4 peptide-immunoglobulin fusion, preferably lea29y, and expresses said ctla4 peptide-immunoglobulin fusion in a tissue-specific manner in pancreatic islets.. .
Mwm Biomodels Gmbh

Assay reagents for a neurogranin diagnostic kit

The present invention relates to the field of biomarkers. More specifically, the present invention relates to assay reagents useful in detecting neurogranin.
The Johns Hopkins University

Compositions and methods for determining likelihood of twinning

Collections of polynucleotides useful for estimating breeding value or detecting likelihood of twinning are disclosed. The polynucleotides are used to detect genomic sequences quantitatively associated with the twinning trait.
Wisconsin Alumni Research Foundation

Compositions and methods of engineered crispr-cas9 systems using split-nexus cas9-associated polynucleotides

The present specification discloses engineered type ii crispr-cas9 systems comprising split-nexus cas9-associated polynucleotides (sn-caspns), including systems comprising three split-nexus cas9-associated polynucleotides (sn1-caspn/sn2-caspn/sn3-caspn) and systems comprising two split-nexus cas9-associated polynucleotides (sn1-caspn/sn2-caspn). Together with a cas9 protein, the sn-caspns facilitate site-specific modifications, including cleavage and mutagenesis, of a target polynucleotide in vitro and in vivo.
Caribou Biosciences, Inc.

Maize genes for controlling plant growth and organ size and their use in improving crop plants

The present invention provides polynucleotides and related polypeptides of the zmargos gene family. The invention provides genomic sequence for the zmargos genes.
Pioneer Hi-bred International, Inc.

Therapeutic

The invention encompasses recombinant polynucleotides, compositions and methods for interfering with antibiotic resistance genes, and/or replicons carrying such genes, in microorganisms in order to disable antibiotic resistance in the microorganisms, using a clustered regularly interspaced short palindromic repeat (crispr) array system.. .
Nemesis Bioscience Ltd

Compositions and methods for virus control in varroa mite and bees

Compositions and methods for providing viral control in varroa mites and bees using rna interference technology, and more particularly, prevention and treatment of viral infections in varroa mites and bees by providing trigger polynucleotides targeting viral sequences is disclosed.. .
Beeologics, Inc.

Polypeptides having cellobiohydrolase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having cellobiohydrolase activity, catalytic domains, and cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains, and cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, or cellulose binding domains..
Novozymes, Inc.

Antibody constructs for flt3 and cd3

The present invention relates to a bispecific antibody construct comprising a first binding domain which binds to human flt3 on the surface of a target cell and a second binding domain which binds to human cd3 on the surface of a t cell. Moreover, the invention provides a polynucleotide encoding the antibody construct, a vector comprising said polynucleotide and a host cell transformed or transfected with said polynucleotide or vector.
Amgen Research (munich) Gmbh

Polypeptides and polynucleotides, and uses thereof as a drug target for producing drugs and biologics

This invention relates to a novel target for production of immune and non-immune based therapeutics and for disease diagnosis. More particularly, the invention provides therapeutic antibodies against vsig1, ildr1, loc253012, ai216611, c1orf32 or fxyd3 antigens, which are predicted co-stimulatory family members and which are differentially expressed in cancers including, lung cancer, ovarian cancer, and colon cancer, and diagnostic and therapeutic usages.
Compugen Ltd.

Anti-cd154 antibodies and methods of using them

The present invention relates to antagonistic antibodies specifically binding cd154, polynucleotides encoding the antibodies or fragments, and methods of making and using the foregoing.. .
Janssen Biotech, Inc.

Antibody constructs for dll3 and cd3

The present invention relates to a bispecific antibody construct comprising a first binding domain which binds to human dll3 on the surface of a target cell and a second binding domain which binds to human cd3 on the surface of a t cell. Moreover, the invention provides a polynucleotide encoding the antibody construct, a vector comprising said polynucleotide and a host cell transformed or transfected with said polynucleotide or vector.
Amgen Research (munich) Gmbh

Antibody therapeutics that bind cd147

There is disclosed compositions and methods relating to or derived from anti-cd147 antibodies. More specifically, there is disclosed fully human antibodies that bind cd147, cd147-binding fragments and derivatives of such antibodies, and cd147-binding polypeptides comprising such fragments.
Sorrento Therapeutics, Inc.

Use of annexin a3 as a diagnostic and prognostic biomarker and therapeutic target for treating hepatocellular carcinoma

Annexin a3 (anax3) is utilized as a biomarker for the diagnosis and prognosis of hepatocellular carcinoma (hcc) and the utilization of a monoclonal antibody against anxa3 or antisense polynucleotide against anxa3 mrna for the suppression or treatment of hcc, alone or in combination with other hcc treatment. Monoclonal antibody against anxa3 can be administered for the suppression of tumor growth, metastasis, and chemoresistance..
The University Of Hong Kong

Recombinant expression of multiprotein complexes using polygenes

The present invention relates to a recombinant polynucleotide encoding a polygene coding for at least three polypeptides wherein at least one of the genes constituting the polygene is of non-viral origin, at least two of the polypeptides encoded by the genes constituting the polygene are each capable of at least transiently interacting with at least one other polypeptide encoded by a gene of said polygene, and the genes constituting the polygene are each connected to one another by a sequence coding for at least one protease cleavage site. The present invention also relates to polyproteins encoded by the polygene.
Eth Zurich

Methods and compositions for treating huntington's disease

Disclosed herein are compositions comprising non-naturally occurring zinc finger domains, fusion proteins comprising these zinc finger domains, polynucleotides encoding these proteins, cells expressing these proteins and pharmaceutical compositions comprising these proteins or polynucleotides as well as methods of modifying an htt gene using these compositions for treating or preventing huntington's disease.. .
Sangamo Biosciences, Inc.

Small molecule binding pockets in nucleic acids

Described herein is technology for determining the 2-d or 3-d atomic resolution structure of a polynucleotide bound to and/or interacting with another molecule, for example a small molecule. In some aspects of the technology, nmr and isotopic labeling strategies are used.
Nymirum, Inc.

Methods for treating textiles using polypeptides having endoglucanase activity

The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, carbohydrate binding modules and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding modules..
Novozymes A/s

Optimization of dna amplification reactions

The present invention relates to methods and uses where a combination of (i) an enzyme exhibiting 5′->3′ exonuclease activity and specifically degrading 5′-phosphorylated strands of double-stranded nucleic acids is used, and (ii) one or more primer pair(s), each primer being 5′ phosphorylated, in a method comprising at least a first and a second amplification reaction of a first and a second template, respectively, for preventing contamination of the second amplification reaction with amplification products of the first amplification reaction, as well as novel methods and kits using the combination. Also, the present invention relates to kits comprising the enzyme and either 5′-phosphorylated primers or a polynucleotide kinase..
Qiagen Gmbh

Corn event mon 87411

The invention provides corn event mon 87411, and plants, plant cells, seeds, plant parts, and commodity products comprising event mon 87411. The invention also provides polynucleotides specific for event mon 87411 and plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides specific for event mon 87411.
Monsanto Technology Llc

Methods and compositions for selective regulation of protein expression

The invention provides novel recombinant dna molecules, compositions, and methods for selectively regulating the expression of a transcribable polynucleotide molecule or recombinant protein in a male reproductive tissue of a transgenic plant. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising such dna molecules and compositions..
Monsanto Technology Llc

Method for improving crop productivity

Plants and plant cells derived therefrom are disclosed having one or more improved or introduced desirable traits such as increased plant biomass including both increased shoot and root growth and increased seed yield. Also disclosed is a method for the production of plants with increased cold tolerance, salt tolerance, drought tolerance, and resistance to parasitic plants.

Method for producing useful protein using plant

Provided is a method for producing a protein with a plant, comprising a step of infecting the plant with agrobacterium having a polynucleotide encoding a target protein, and a step of expressing the target protein in the plant by further cultivating the plant after infection, wherein production efficiency of the target protein is improved by imparting a damaging stimulus to the roots of the plant.. .
Mitsubishi Chemical Corporation

Chimeric and proline rich protein promoters for expression in plants

The present invention provides regulatory polynucleotide molecules isolated from plant proline rich protein genes and linked to a viral enhancer molecule. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the regulatory polynucleotide sequences, and methods for preparing and using the same..
Monsanto Technology Llc

Selection in fungi

The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5′ untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.. .
Novozymes A/s

Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of histidyl-trna synthetases

Provided are compositions comprising newly identified protein fragments of aminoacyl-trna synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications.. .
Pangu Biopharma Limited

Innovative discovery of therapeutic, diagnostic, and antibody compositions related protein fragments of tryptophanyl trna synthetases

Provided are compositions comprising newly identified protein fragments of aminoacyl-trna synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications.. .
Pangu Biopharma Limited

Polypeptides having protease activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having protease activity, and polynucleotides encoding the polypeptides. The invention further relates to the use of such polypeptides in detergent and/or in cleaning processes.
Novozymes A/s

Codon-optimized gene for mutated shrimp luciferase and use thereof

There has been a demand for a codon-optimized gene for the mutated catalytic domain of oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and escherichia coli. There has also been a demand for a substrate coelenterazine analogue showing a higher activity than that of native 19 kda protein.
Jnc Corporation

Polypeptides having peroxygenase activity and polynucleotides encoding same

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
Novozymes A/s

Antibody constructs for egfrviii and cd3

The present invention relates to a bispecific antibody construct comprising a first binding domain which binds to human egfrviii on the surface of a target cell and a second binding domain which binds to human cd3 on the surface of a t cell. Moreover, the invention provides a polynucleotide encoding the antibody construct, a vector comprising said polynucleotide and a host cell transformed or transfected with said polynucleotide or vector.
Amgen Research (munich)

Antibody constructs for msln and cd3

The present invention relates to a bispecific antibody construct comprising a first binding domain which binds to human msln on the surface of a target cell and a second binding domain which binds to human cd3 on the surface of a t cell. Moreover, the invention provides a polynucleotide encoding the antibody construct, a vector comprising said polynucleotide and a host cell transformed or transfected with said polynucleotide or vector.
Amgen Inc.

Antigen binding constructs

The present invention is directed to antigen binding constructs comprising one or two epitope binding domains separated by a single chain fc region of an antibody, wherein each epitope binding domain in capable of binding to vegf, to dimers comprising two antigen binding constructs of the invention, pharmaceutical compositions comprising said dimers and their use in the treatment of diseases associated with vegf signalling, such as diabetic macular edema (dme), wet age-related macular degeneration (wet amd), diabetic retinopathy, retinal vein occlusion (rvo), and corneal neovascularisation, and polynucleotide sequences encoding said antigen binding constructs.. .
Glaxo Group Limited

Modified polynucleotides for the production of nuclear proteins

The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmrna molecules.. .
Modernatx, Inc.

Use of liposomes in a carrier comprising a continuous hydrophobic phase for delivery of polynucleotides in vivo

The invention provides compositions comprising a carrier comprising a continuous phase of a hydrophobic substance, liposomes, and a polynucleotide, and methods for using such compositions for delivering a polynucleotide to a subject.. .
Immunovaccine Technologies Inc.

Dna vaccines and methods for the prevention of transplantation rejection

Methods for preventing, delaying the onset of, or treating rejection of an allograft using a dna vaccine, where the vaccine can comprise a polynucleotide encoding a pro-apoptotic protein, like bax and/or a polynucleotide encoding an autoantigen or donor antigen, like secreted glutamic acid decarboxylase 55. Administering one of the dna vaccines to a transplant recipient, as described herein, can induce a donor specific tolerogenic response..
Loma Linda University

Compositions associated with soybean reproductive growth and methods of use

Molecular markers associated with soybean reproductive stage, methods of their use, and compositions having one or more marker loci are provided. Methods comprise detecting at least one marker locus, detecting a haplotype, and/or detecting a marker profile.
Pioneer Hi-bred International, Inc.

High mast2-affinity polypeptides and uses thereof

The invention relates to polypeptides containing a cytoplasmic domain ending with a mast-2 binding domain, from 11 to 13 residues, the first two residues of which are s and w, and the last four residues of which are q, t, r and l, the polypeptides presenting a high affinity for the pdz domain of the human mast2 protein. The invention also relates to polynucleotides, vectors, lentiviral particles, cells as well as compositions containing the same.
Ip

Method of target molecule characterisation using a molecular pore

The invention relates to a new method of determining the presence, absence or one or more characteristics of multiple analytes. The invention concerns coupling a first analyte to a membrane containing a detector and investigating the first analyte using the detector.
Oxford Nanopore Technologies Ltd.

Polynucleotide sequencing methods

Methods for determining the sequence of a polynucleotide comprising the steps of (i) contacting a polynucleotide processive enzyme immobilised in a fixed position, with a target polynucleotide under conditions sufficient to induce enzyme activity; (ii) detecting an effect consequent on the interaction of the enzyme and the polynucleotide, wherein the effect is detected by measurement of a non-linear optical signal or a linear signal coupled to a non-linear signal.. .
Gen-probe Incorporated

Detection and quantification of methylation in dna

Provided are methods and systems for characterizing a biomolecular parameter of a polynucleotide. A polynucleotide of interest from a sample comprising a heterogeneous mixture of polynucleotides is concentrated and provided to a first fluid compartment of a solid-state nanopore.
Mayo Foundation For Medical Education And Research

Engineered imine reductases and methods for the reductive amination of ketone and amine compounds

The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.. .
Codexis, Inc.

Hybrid polypeptides having cellobiohydrolase activity and polynucleotides encoding same

The present invention relates to hybrid polypeptides having cellobiohydrolase activity. The present invention also relates to polynucleotides encoding the hybrid polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and processes of using the hybrid polypeptides..
Novozymes, Inc.

Compositions and methods comprising sequences having meganuclease activity

Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences.
Pioneer Hi-bred International, Inc.

Engineered biocatalysts and methods for synthesizing chiral amines

The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.. .
Codexis, Inc.

Antibody therapeutics that bind lag3

There is disclosed compositions and methods relating to or derived from anti-lag3 antibodies. More specifically, there is disclosed fully human antibodies that bind lag3, lag3-antibody binding fragments and derivatives of such antibodies, and lag3-binding polypeptides comprising such fragments.
Sorrento Therapeutics, Inc.



Polynucleotide topics:
  • Nucleotide
  • Polynucleotide
  • Polypeptide
  • Antagonist
  • Nucleic Acid
  • Cancer Cell
  • Drug Resistance
  • Chromosome
  • Glycoprotein
  • Gastrointestinal
  • Gastrointestinal Stromal Tumors
  • Gastrointestinal Stromal Tumor
  • Cellobiohydrolase
  • Human Disease
  • Inflammation


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    This listing is a sample listing of patent applications related to Polynucleotide for is only meant as a recent sample of applications filed, not a comprehensive history. There may be associated servicemarks and trademarks related to these patents. Please check with patent attorney if you need further assistance or plan to use for business purposes. This patent data is also published to the public by the USPTO and available for free on their website. Note that there may be alternative spellings for Polynucleotide with additional patents listed. Browse our RSS directory or Search for other possible listings.


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