This page is updated frequently with new Polynucleotide-related patent applications.
|| List of recent Polynucleotide-related patents
|Complex sets of mirnas as non-invasive biomarkers for colon cancer|
Described herein are non-invasive methods, kits and means for diagnosing and/or prognosing of colon cancer a body fluid sample from a subject. Further described herein are sets of polynucleotides or sets of primer pairs for detecting sets of mirnas for diagnosing and/or prognosing of colon cancer in a body fluid sample from a subject.
Comprehensive Biomarker Center Gmbh
|Method and device for identifying nucleotide, and for determining nucleotide sequence of polynucleotide|
The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value.
|Improved polynucleotide sequences encoding tale repeats|
The present invention is in the field of the gene editing molecular tools. The present invention relates to rewritten nucleic acid sequences encoding repeated dna recognition motifs of tale (transcription activator-like effector) proteins.
|Compositions and methods directed to crispr/cas genomic engineering systems|
The invention relates to engineered crispr/cas9 systems for genomic modification in mammalian cells. The present specification describes the design and testing of a polynucleotide encoding the streptococcus pyogenes (s.
System Biosciences, Llc
|Wheat stem rust resistance gene|
The present invention relates to a transgenic plant which has integrated into its genome an exogenous polynucleotide encoding a polypeptide which confers resistance to puccinia graminis f sp. Tritici, such as the ug99 group of races puccinia graminis f.
Commonwealth Scientific And Industrial Research Organisation
|Methods of increasing abiotic stress tolerance and/or biomass in plants|
Provided are methods of increasing the tolerance of a plant to abiotic stresses and/or increasing the biomass and/or increasing the yield of a plant by expressing within the plant an exogenous polynucleotide encoding a polypeptide homologous to seq id no:240, such as the polynucleotide set forth by seq id no:14.. .
|Novel genes involved in biosynthesis|
The invention provides a novel myb class transcription factor gene (nucleic acid sequences, protein sequences, and variants and fragments thereof) designated myb14 by the applicants, that is useful for manipulating the production of flavonoids, specifically condensed tannins, in plants. The invention provides the isolated nucleic acid molecules encoding proteins with at least 70% identity to any one of myb14 polypeptide sequences of seq id no: 14 and 46 to 54.
Grasslanz Technology Limited
|Treatment of brain derived neurotrophic factor (bdnf) related diseases by inhibition of natural antisense transcript to bdnf|
The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of brain derived neurotrophic factor (bdnp), in particular, by targeting natural antisense polynucleotides of brain derived neurotrophic factor (bdnf). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of bdnf..
|Subtilase variants and polynucleotides encoding same|
The present invention relates to novel subtilase variants exhibiting increased stability and optionally on par or improved wash performance. The variants of the invention are suitable for use in e.g.
|Stabilized receptor polypeptides and uses thereof|
The present invention provides stabilized activin iib receptor polypeptides and proteins capable of binding and inhibiting the activities of activin a, myostatin, or gdf-11. The present invention also provides polynucleotides, vectors and host cells capable of producing the stabilized polypeptides and proteins.
Cosmetic composition for improving skin conditions comprising fusion protein
The present invention relates to a fusion protein comprising a skin-penetrating peptide, a polynucleotide encoding the fusion protein, an expression vector comprising the polynucleotide, a transformant comprising the expression vector, a method for preparing the fusion protein, a cosmetic composition for improving skin conditions, which comprises the fusion protein, and a pharmaceutical composition for external skin use, which comprises the fusion protein. The fusion protein of the invention comprises a skin-penetrating peptide bound to a physiologically active protein.
Lg Household & Health Care Ltd.
Structure, manufacturing and uses of human-derived cell-permeable peptides conjugated with specific biologically active cargo peptides
Embodiments disclosed herein provide compositions for conjugates, including fusion proteins, and methods of using them to treat a variety of conditions. In some embodiments, the conjugates and/or fusion proteins incorporate a 60-amino acid human homeodomain (e.g., peptides derived from human hox genes), to translocate functional and regulatory peptides and proteins or other biologically active molecules such as nucleic acids, which are not naturally associated with the human homeodomain, across cell and nuclear membranes to intended sites of action without provoking an unwanted immune response that may reduce exposure to the conjugate and/or result in a clinical adverse event.
Portage Pharmaceuticals Ltd.
Vaccines against pregnancy-associated malaria
The present invention relates to combinations of polypeptides or of polynucleotides corresponding to a specific region of the n-terminal portion of the var2csa protein of different parasitic families or lines of plasmodium falciparum, and to their use in the prevention of pregnancy-associated malaria. The invention also relates to immunogenic compositions and to vaccines useful for preventing malaria in pregnant women..
Institut De Recherche Pour Le DÉvelopment (ird)
An immunity-inducing agent comprising as an effective ingredient(s) a polypeptide(s) selected from the polypeptides: (a) a polypeptide consisting essentially of not less than 7 consecutive amino acids in any one of the amino acid sequences shown in seq id nos:2, 4, 6, 8, 10, 12 and 44 in sequence listing; (b) a polypeptide having a sequence identity of not less than 90% with the polypeptide (a) and consisting essentially of not less than 7 amino acids; and (c) a polypeptide comprising the polypeptide (a) or (b) as a partial sequence thereof; which polypeptide(s) has/have an immunity-inducing activity/activities, or as an effective ingredient(s) a recombinant vector(s) which comprise(s) a polynucleotide(s) encoding the polypeptide(s) and is/are capable of expressing the polypeptide(s) in vivo, is useful as a therapeutic and/or prophylactic agent for cancer, and/or the like.. .
Toray Industries, Inc.
Compositions and methods of altering cholesterol levels
The present invention relates to compositions, methods and kits using polynucleotides, primary transcripts and mmrna molecules. The present invention also relates to compositions and methods for altering cholesterol levels using polynucleotides, primary transcripts and mmrna molecules..
Moderna Therapeutics, Inc.
Methods and compositions for the diagnosis and treatment of cancer
The present invention relates to methods, kits, and compositions for detecting and/or diagnosing metastatic potential of cancer cells or for evaluating prognosis in a patient with cancer by detection of the protein expression level of an hla class i molecule and/or the copy number variation of a polynucleotide encoding the hla class i molecule. The present invention also relates to the use of the protein expression level of an hla class i molecule and/or the copy number variation of a polynucleotide encoding the hla class i molecule as a prognosis biomarker and metastasis predictive biomarker of cancer..
Beijing Institute For Cancer Research
Polynucleotide probe, detecting a target nucleic acid by using the same and kit comprising the same
The present invention provides a method for detecting a target nucleic acid that comprises a step of providing a sample; contacting the sample with a polynucleotide probe comprising a first sequence and a second sequence complementary to the target nucleic acid; and adding a nuclease for cleaving the second sequence of the polynucleotide probe. The present invention further provides a polynucleotide probe for detecting a target nucleic acid that comprises a first sequence and a second sequence complementary to the target nucleic acid.
National Taiwan University
Expression of natively secreted polypeptides without signal peptide
The present invention relates to methods of recombinantly producing a natively secreted polypeptide, the method comprising the steps of providing a microorganism host cell comprising an exogenous polynucleotide encoding a natively secreted polypeptide without a translationally fused signal peptide; cultivating the microorganism host cell under conditions conducive to the expression of the polypeptide and, optionally, recovering the polypeptide, as well as microorganisms, certain polynucleotides, expression constructs and protease substitution variants.. .
Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion
The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells..
Butamax Advanced Biofuels Llc
Regulation of gene expression
The invention provides isolated polynucleotides comprising sequences encoding a u orf peptides and variants and fragments thereof. The invention also provides constructs and vectors containing the polynucleotides.
The New Zealand Institute For Plant And Food Research Limited
Filamentous fungal mutants with improved homologous recombination efficiency
The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for nhr, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards hr. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an hr pathway with elevated efficiency and/or an nhr pathway with a lowered efficiency and/or a nhr/hr ratio with decreased efficiency as compared to said hr and/or nhr efficiency and/or nhr/hr ratio of said parent cell under the same conditions..
Dsm Ip Assets B.v.
Aptamers for the treatment of sickle cell disease
The present invention provides polynucleotide aptamers that selectively bind to and inhibit polymerization of sickle hemoglobin (hbs), pharmaceutical compositions comprising the same, methods of use for diagnostics and treatment of sickle cell disease, methods of use as capture reagents, and methods of rational drug design.. .
The Johns Hopkins University
Treatment of alpha-l-iduronidase (idua) related diseases by inhibition of natural antisense transcript to idua
The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of alpha-l-iduronidase (idua), in particular, by targeting natural antisense polynucleotides of alpha-l-iduronidase (idua). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of idua..
Alpha amylase variants and polynucleotides encoding same
The present invention relates to variants of alpha amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants..
A laglidadg homing endonuclease cleaving the t cell receptor alpha gene and uses thereof
Disclosed herein are compositions for inactivating the human tcr-alpha gene comprising engineered laglidadg homing endonucleases (lhes) and their derivatives, particularly derived from members of the \-onul subfamily of lhes. Polynucleotides encoding such endonucleases, vectors comprising said polynucleotides, cells comprising or having been treated with such endonucleases, and therapeutic compositions deriving therefore are also provided..
Biocatalysts and methods for the synthesis of armodafinil
The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (−)-2-[(r)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (r)-2-(benzhydrylsulfinyl)acetic acid, which is a pivotal intermediate in the synthesis of armodafinil, in enantiomeric excess..
Antibodies to human signal peptide-containing proteins
The invention provides a human signal peptide-containing proteins (sigp) and polynucleotides which identify and encode sigp. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists.
Optimized non-canonical zinc finger proteins
Disclosed herein are zinc fingers comprising cchc zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these cchc zinc fingers as well as polynucleotides encoding these proteins.
Sangamo Biosciences, Inc.
Novel rhtb protein variants and the producing o-phosphoserine using the same
The present invention relates to an rhtb (homoserine/homoserine lactone export transporter) protein variant having an enhanced ability to export o-phosphoserine (ops) that is a precursor of l-cysteine, a polynucleotide encoding the protein, a vector comprising the polynucleotide, an ops-producing microorganism comprising the protein variant, a method of producing o-phosphoserine using the microorganism, and a method for preparing cysteine or its derivatives, which comprises reacting o-phosphoserine, produced by the method above, with a sulfide in the presence of o-phosphoserine sulfhydrylase (opss) or a microorganism that expresses opss.. .
Cj Cheiljedang Corporation
Droplet storage method
A method of storing a stream of droplets at least some of which comprise one or more single nucleotides and/or oligonucleotides, and a droplet fluid is provided. It is characterised by the step of introducing each droplet sequentially onto a surface of a substrate at a corresponding unique location and further characterised in that the stream of droplets is prepared by a process which includes the steps of generating an ordered stream of nucleotides from the analyte by progressive pyrophosphorolysis or exo nucleolysis and capturing each nucleotide in a corresponding droplet.
Base4 Innovation Ltd
Gene expression system using alternative splicing in insects
A polynucleotide expression system is provided that is capable of alternative splicing of rna transcripts of a polynucleotide sequence to be expressed in an organism.. .
A producing precise dna cleavage using cas9 nickase activity
The present invention is in the field of a method for genome engineering based on the type ii crispr system, particularly a method for improving specificity and reducing potential off-site. The method is based on the use of nickase architectures of cas9 and single or multiple crrna(s) harboring two different targets lowering the risk of producing off-site cleavage.
Production of polypeptides without secretion signal in bacillus
The present invention relates to a method of producing a natively non-secreted polypeptide without a secretion signal in a bacillus host cell and recovering the polypeptide without performing a lysis step as well as to a bacillus host cell comprising one or more exogenous or heterologous polynucleotides encoding a natively non-secreted polypeptide with no secretion signal.. .
Aspects of the present compositions and methods relate to novel metalloproteases, polynucleotides encoding the novel metalloproteases, and compositions and methods for use thereof.. .
Danisco Us Inc.
Xylanase variants and polynucleotides encoding same
The present invention relates to xylanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants..
Homeodomain fusion proteins and uses thereof
Provided herein are fusion proteins comprising a homeodomain fusion protein domain and a transcription modulator domain for treatment of various diseases or disorders such as cancer. The homeodomain fusion protein domain binds to a target gene and the transcription modulator domain either activates or represses gene transcription.
The General Hospital Corporation D/b/a Massachusetts General Hospital
Dig-303 insecticidal cry toxins
Dig-303 insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.. .
Dow Agrosciences Llc
Polypeptides having nucleic acid binding activity
Polynucleotides having nucleic acid binding activity are provided. Methods of stabilizing a nucleic acid duplex are provided.
Applied Biosystems, Llc
Coagulation factor vii polypeptides
The present invention relates to modified coagulation factor vii polypeptides exhibiting increased resistance to antithrombin inactivation and enhanced proteolytic activity. The present invention also relates to polynucleotide constructs encoding such polypeptides, vectors and host cells comprising and expressing such polynucleotides, pharmaceutical compositions, uses and methods of treatment..
Novo Nordisk Healthcare Ag
Therapeutic agents comprising elastin-like peptides
The present invention provides therapeutic agents and compositions comprising elastin-like peptides (elps) and therapeutic proteins. In some embodiments, the therapeutic protein is a glp-1 receptor agonist, insulin, or factor vii/viia, including functional analogs.
Stable neural stem cells comprising an exogenous polynucleotide coding for a growth factor and methods of use thereof
The present disclosure provides a human neural stem cell comprising an exogenous polynucleotide coding for a growth factor such as igf-1. Also disclosed are methods of using the human neural stem cells for the treatment of neurodegenerative diseases or disorders including, for example, als..
Methods for engineering highly active t cell for immunotheraphy
The present invention relates to methods for developing engineered t-cells for immunotherapy and more specifically to methods for modifying t-cells by inactivating at immune checkpoint genes, preferably at least two selected from different pathways, to increase t-cell immune activity. This method involves the use of specific rare cutting endonucleases, in particular tale-nucleases (tal effector endonuclease) and polynucleotides encoding such polypeptides, to precisely target a selection of key genes in t-cells, which are available from donors or from culture of primary cells.
Methods for engineering allogeneic and highly active t cell for immunotheraphy
The present invention relates to methods for developing engineered t-cells for immunotherapy that are non-alloreactive. The present invention relates to methods for modifying t-cells by inactivating both genes encoding t-cell receptor and an immune checkpoint gene to unleash the potential of the immune response.
Compositions and methods for minimizing nornicotine synthesis in tobacco
Compositions and methods for reducing the level of nornicotine and n′-nitrosonornicotine (nnn) in tobacco plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for a root-specific nicotine demethylases, cyp82e10, and variants thereof, that are involved in the metabolic conversion of nicotine to nornicotine in these plants.
North Carolina State University
Molecular barcoding for multiplex sequencing
Described herein are methods, compositions and kits for preparing samples for multiplex next generation nucleic acid sequencing. The methods entail the use of in-line barcodes that minimize barcode-confusing chimeras, purification procedures with low cost, and/or a quantitative amplification to generate a desired amount of polynucleotides for sequencing..
Athena Diagnostics, Inc.
Methods for estimating the size of disease-associated polynucleotide repeat expansions in genes
Methods for estimating the size of disease-associated polynucleotide repeat expansions in genes are disclosed which use restriction enzymes that do not cut within a repeat expansion and which are frequent cutting restriction enzymes that cut genomic dna outside of the expansion into fragments of a size below the threshold capable of detection. A hybridisation probe that can bind to multiple sites within the expansion is then used to estimate its length and to correlate that to the diagnosis or prognosis of disease..
Medical Research Council
Mutant protease biosensors with enhanced detection characteristics
A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the c-terminal portion of the thermostable luciferase to the n-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase.
Novel o-phosphoserine export protein and the producing o-phosphoserine using the same
The present invention relates to a novel isolated polypeptide having the ability to export o-phosphoserine (ops) that is a precursor of l-cysteine, a vector comprising the polynucleotide, an ops-producing microorganism having enhanced activity of the polypeptide, a method of producing ops using the microorganism, and a method for preparing cysteine or its derivatives, which comprises reacting ops, produced by the above method, with a sulfide in the presence of o-phosphoserine sulfhydrylase (opss) or a microorganism that expresses opss.. .
Cj Cheiljedang Corporation
Direct transfer of polynucleotides between genomes
The present invention provides methods for directly transferring a recombinant polynucleotide of interest from the chromosome of a prokaryotic donor cell to the chromosome of a prokaryotic recipient cell of another species, wherein the polynucleotide of interest comprises at least one coding sequence of interest, preferably one gene of interest and a selectable marker, wherein said polynucleotide of interest in the chromosome of the donor cell is flanked on each side by a polynucleotide region derived from the chromosome of the recipient cell, and wherein the flanking polynucleotide regions are of sufficient size and sequence identity to allow double homologous recombination with the corresponding regions in the chromosome of the recipient cell.. .
Compositions and methods of use
The present compositions and methods relate to a beta-mannanase from bacillus hemicellulosilyticus, polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (ggm) and/or glucomannan (gm)..
Danisco Us Inc.
Codon modified amylase from bacillus akibai
The present invention relates to an isolated synthetic polynucleotide encoding the mature amylase ax856 from bacillus akibai, using codon modified polynucleotide constructs for the expression of the amylase.. .
Mutant dna polymerases and methods of use
The present invention provides mutant dna polymerases, polynucleotides encoding the polymerases, cassettes and vectors including such polynucleotides, and cells containing the polymerases, polynucleotides, cassettes, and/or vectors of the invention. The present invention also provides methods for synthesizing polynucleotides and kits including a dna polymerase of the invention..
Applied Biosystems, Llc
Chimeras of brucella lumazine synthase and beta subunit of ab5 toxins
Chimeric polypeptides useful as immunogens for inducing protective immune responses and neutralizing antibodies against shiga toxin (stx) in mammals. More specifically, chimeric polypeptides having a monomer of the homopentameric b subunit of the shiga 2 toxin fused to the n-terminus of a monomer of brucella lumazine synthase, and to oligomeric protein complexes formed from said chimeric polypeptides.
Amine-containing lipidoids and uses thereof
Provided herein are lipidoids that may be prepared from the conjugate addition of alkylamines to acrylates. In some embodiments, provided lipidoids are biodegradable and may be used in a variety of drug delivery systems.
Massachusetts Institute Of Technology
Pan-dr binding polypeptides and uses thereof
The present invention provides novel artificial oligopeptides capable of binding hla class ii molecules encoded by several alleles. The oligopeptides include the sequence ax1fvaax2tlx3ax4a (seq id no:1), wherein x1 is selected from the group consisting of w, f, y, h, d, e, n, q, i and k; x2 is selected from the group consisting of f, n, y and w; x3 is selected from the group consisting of h and k, and x4 is selected from the group consisting of a, d and e, with the proviso that the oligopeptide sequence is not akfvaawtlkaaa.
Imp-3 epitope peptides for th1 cells and vaccines containing the same
Isolated imp-3-derived epitope peptides having th1 cell inducibility are disclosed herein. Such peptides can be recognized by mhc class ii molecules and induce th1 cells.
Oncotherapy Science, Inc.
Compositions and methods comprising glycyl-trna synthetases having non-canonical biological activities
Isolated glycyl-trna synthetase polypeptides and polynucleotides having non-canonical biological activities are provided, as well as compositions and methods related thereto.. .
Atyr Pharma, Inc.
Modified polynucleotides for the production of nuclear proteins
The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmrna molecules.. .
Moderna Therapeutics, Inc.
Composition for preventing or treating autoimmune disease, comprising, as active ingredient, pink1 protein or polynucleotide encoding same
The present invention relates to a composition for preventing or treating an autoimmune disease comprising, as an active ingredient, pink1 protein or polynucleotide encoding the same. The pink1 protein according to the present invention inhibits the activity of th17, promotes the activity of a regulatory t cell (treg), and increases autophagy in treg cells, thereby controlling excessive immune responses.
Catholic University Industry Academic Cooperation Foundation
Novel nylanderia pubens virus
At least one novel virus capable of infecting crazy ants (nylanderia fulva) is isolated, along with polynucleotide sequences and amino acid sequences of the virus. The virus is capable of be used as a biopesticide to control populations of crazy ants..
The United States Of America As Represented By The Secretary Of Agriculture
Biological specimen collection and transport system
Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay.
Photocatalytic hydrogen production and polypeptides capable of same
An isolated polypeptide comprising a hydrogen generating enzyme attached to a heterologous ferredoxin is disclosed, as well as polynucleotides encoding same, nucleic acid constructs capable of expressing same and cells expressing same. A method for generating hydrogen using the isolated polypeptide is also disclosed..
Proteins toxic or inhibitory to lepidopteran insects
Nucleotide sequences are disclosed encoding novel, insecticidal tic2160 and tic3244 proteins, and variants thereof, and related proteins exhibiting lepidopteran inhibitory activity, as well as fragments thereof. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing a polynucleotide construct encoding one or more of the toxin proteins within the tic2160-related protein toxin class..
Lepidopteran-active cry1da1 amino acid sequence variant proteins
Engineered cry1da amino acid sequences are provided that exhibit improved lepidopteran insecticidal activity and an enhanced lepidopteran spectrum compared to the naturally occurring cry1da protein toxin. polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided.
Compositions and methods for insecticidal control of stinkbugs
Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pentatomidae plant pest, decrease the expression of a target sequence in the pest. The present invention provides various target polynucleotides set forth in any one of seq id nos: 6-12, 18-40 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity).
Manipulation of glutamine symthetases (gs) to improve nitrogen use efficiency and grain yield in higher plants
The present invention provides polynucleotides and related polypeptides of the protein gs. The invention provides genomic sequence for the gs gene.
Modified frt recombination site libraries and methods of use
Methods and compositions using populations of randomized modified frt recombination sites to identify, isolate and/or characterize modified frt recombination sites are provided. Kits comprising the library populations of frt sites are also provided, as are methods to make a library of modified frt recombination sites.
Chimeric single-stranded antisense polynucleotides and double-stranded antisense agent
Chimeric single-stranded polynucleotides and double-stranded antisense agents useful for modifying the expression of a target gene by means of an antisense effect are disclosed. The chimeric single-stranded antisense polynucleotide and double-stranded antisense agents comprise a central nucleotide region flanked by a first 5′-wing region and a first 3′-wing region of modified nucleotides, which are themselves flanked by a second 5′-wing region and/or a second 3′-wing region of nucleotides that have a low affinity for proteins and/or that have higher resistance to dnase or rnase than a natural dna or rna and are missing in a cell when the chimeric polynucleotide delivered.
Aspects of the present compositions and methods relate to novel metalloproteases polynucleotides encoding the novel metalloprotease, compositions and methods for use thereof.. .
Aspects of the present compositions and methods relate to novel metalloproteases polynucleotides encoding the novel metalloprotease, compositions and methods for use thereof.. .
Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
The invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, β-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts.
Amylases, nucleic acids encoding them and methods for making and using them
In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars..
Hydrolases, nucleic acids encoding them and methods for making and using them
Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils..
Actriib-fc polynucleotides, polypeptides, and compositions
In certain aspects, the present invention provides compositions and methods for modulating (promoting or inhibiting) growth of a tissue, such as bone, cartilage, muscle, fat, and/or neuron. The present invention also provides methods of screening compounds that modulate activity of an actrii protein and/or an actrii ligand.
Immunity induction agent
Provided is a method for inducing immunity for therapy of a cancer(s). The method includes the step of administering to an individual with cancer at least one polypeptide selected from the polypeptides (a) or (b) below, and/or a recombinant vector(s) that comprise(s) a polynucleotide(s) encoding the at least one polypeptide, the recombinant vector(s) being capable of expressing the polypeptide(s) in vivo: (a) a polypeptide in any one of the amino acid sequences of seq id nos: 2, 4, 22, or 24; and (b) a polypeptide having a sequence identity of not less than 95% to the polypeptide (a).
Methods for detecting a mycobacterium tuberculosis infection
Methods for detecting an infection with mtb in a subject are disclosed. The methods include detecting the presence of cd8+ t cells that specifically recognize an mtb polypeptide.
Apparatuses, methods, systems, and computer-readable media for fluid potential artifact correction in reagent delivery systems
A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.. .
Polymorphisms associated with age-related macular degeneration and methods for evaluating patient risk
The present invention provides for certain polynucleotide sequences that have been correlated to amd. These polynucleotides are useful as diagnostics, and are preferably used to fabricate an array, useful for screening patient samples.
Methods for sequencing polynucleotides
The invention relates to methods and systems for sequencing and constructing a high resolution physical map of a polynucleotide. In accordance with the invention, nucleotide sequences are determined at the ends of restriction fragments produced by a plurality of digestions with a plurality of combinations of restriction endonucleases so that a pair of nucleotide sequences is obtained for each restriction fragment.
A laglidadg homing endonuclease cleaving the c-c chemokine receptor type-5 (ccr5) gene and uses thereof
Disclosed herein are compositions for inactivating the human ccr5 gene comprising engineered laglidadg homing endonucleases (lhes) and their derivatives, particularly derived from members of the \-onui subfamily of lhe. Polynucleotides encoding such endonucleases, vectors comprising said polynucleotides, cells comprising or having been treated with such endonucleases, and therapeutic compositions deriving therefrom are also provided..
Methods for producing polypeptides in enzyme-deficient mutants of fusarium venentatum
The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrg, amya, and alpa, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5′-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of fusarium venenatum strains and methods for producing such mutants..
Protection against ionizing radiation and chemotherapy toxicity via latexin regulation
The present invention relates to methods for protecting against damage caused by radiation and/or chemotherapy, and methods for treating bone marrow damage by reducing/inhibiting latexin expression and/or latexin activity. The methods comprise administering to a subject in need thereof a pharmaceutical composition comprising an antagonist that reduces expression and/or activity of latexin, wherein latexin is a latexin polynucleotide variant and/or a latexin polypeptide variant that binds to the antagonist..
Polynucleotide capture materials, and methods of using same
Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as rna and/or dna from such samples. The rna and/or dna is captured by polyamindoamine (pamam (generation 0)) bound to a surface, such as the surface of magnetic particles.
Polypeptides encoding mutated mannanases with improved catalytic efficiency
A polypeptide which is a mutated mannanase, whose sequence is derived from a native mannanase of the filamentous ascomycete coprophile fungus podospora anserina, and being characterised by an increased catalyst efficiency of at least 25%. Also, a polynucleotide encoding the polypeptide, a vector including the polynucleotide, a host cell including the vector or the polynucleotide, and a composition including the polypeptide, the polynucleotide, the vector or the host cell.
Galactose cluster-pharmacokinetic modulator targeting moiety for sirna
The present invention is directed compositions for targeted delivery of rna interference (rnai) polynucleotides to cell in vivo. The pharmacokinetic modulator improve in vivo targeting compared to the targeting ligand alone.
Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
Compositions and methods for reducing the level of nornicotine and n′-nitrosonornicotine (nnn) in nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome p450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants.
Polynucleotide mapping and sequencing
The present invention provides methods of obtaining structural information about a biopolymer sample. The methods include labeling portions of a biopolymer, such as dna or rna, linearizing the biopolymer in some cases, and determining the distance between the labels.
Bionano Genomics, Inc.