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Polymerase Chain Reaction
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Sequencing
Real Time Pcr
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Polymerase Chain Reaction patents



      
           
This page is updated frequently with new Polymerase Chain Reaction-related patents. Subscribe to the Polymerase Chain Reaction RSS feed to automatically get the update: related Polymerase RSS feeds.

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Date/App# patent app List of recent Polymerase Chain Reaction-related patents
04/10/14
20140099644
 Novel compositions, methods and kits for real time polymerase chain reaction (pcr) patent thumbnailNovel compositions, methods and kits for real time polymerase chain reaction (pcr)
The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.. .
04/10/14
20140099636
 Field-based qpcr microbial monitoring patent thumbnailField-based qpcr microbial monitoring
Dna/rna monitoring of microbes at an oil field to determine the presence and activity of harmful microbes is accomplished with a portable qpcr (quantitative polymerase chain reaction) machine. This permits the monitoring to occur on-site in the field and reduces the variability that may occur from the transportation of samples..
04/03/14
20140093942
 Gene, ars-r anchorage cassette, ars-r expression-anchorage cassette, recombinant plasmid, bacterial transgenic lineage, use of said gene, use of said lineage in environmental bioremediation processes patent thumbnailGene, ars-r anchorage cassette, ars-r expression-anchorage cassette, recombinant plasmid, bacterial transgenic lineage, use of said gene, use of said lineage in environmental bioremediation processes
The present invention relates to the construction and insertion of a dna plasmid vector of broad spectrum for gram-negative bacteria, that carries a gene sequence which, when expressed, enables the anchorage of a chelator protein for arsenic ions on the gram-negative bacteria cellular surface. For that end, the structural sequence of the regulatory arsr gene without stop codon (seq id no 1) was amplified by polymerase chain reaction (pcr) using as a template the chromosome 1 of cupriavidus metallidurans, ch34 lineage and inserted into the pgem-t cloning vector, yielding the pgemt-as plasmid (seq id no 2).
04/03/14
20140093872
 Method of mutation detection in blood cell-free dna using primer extension (pe) and pcr patent thumbnailMethod of mutation detection in blood cell-free dna using primer extension (pe) and pcr
A method of detecting mutation in blood cell-free dna, includes providing a serum sample, isolating dna from the serum sample, amplifying the dna by polymerase chain reaction (pcr), subjecting the pcr product to primer extension (pe), and separating the pe reaction product and identifying the mutation by gel electrophoresis. In order to improve accuracy and sensitivity, the pe reaction can be carried out by using a primer that blocks the extension of the wild or non-mutated sequence..
03/20/14
20140080133
 Thermally controlled chamber with optical access for high-performance pcr patent thumbnailThermally controlled chamber with optical access for high-performance pcr
Novel methods and systems for polymerase chain reaction (pcr) are disclosed. A pcr device has a bottom heater layer, a central reacting layer, and a top heater layer.
03/06/14
20140066322
 Mouse cell line authentication patent thumbnailMouse cell line authentication
A multiplex polymerase chain reaction assay that targets nine tetranucleotide short tandem repeat (str) markers in the mouse genome. Unique profiles were obtained from seventy-two mouse samples that were used to determine the allele distribution for each str marker.
03/06/14
20140065702
 Polymerase chain reaction patent thumbnailPolymerase chain reaction
A polymerase chain reaction (pcr) device for a reverse transcriptase reaction (rt) and a convectively-driven polymerase chain reaction (cpcr) in the same device is provided. The pcr device comprises an upper temperature-controlling unit, a middle temperature-controlling unit and a lower temperature-controlling unit.
03/06/14
20140065609
 Varietal counting of nucleic acids for obtaining genomic copy number information patent thumbnailVarietal counting of nucleic acids for obtaining genomic copy number information
A method for obtaining from genomic material genomic copy number information unaffected by amplification distortion, comprising obtaining segments of the genomic material, tagging the segments with substantially unique tags to generate tagged nucleic acid molecules, such that each tagged nucleic acid molecule comprises one segment of the genomic material and a tag, subjecting the tagged nucleic acid molecules to polymerase chain reaction (pcr) amplification, generating tag associated sequence reads by sequencing the product of the pcr reaction, assigning each tagged nucleic acid molecule to a location on a genome associated with the genomic material by mapping the subsequence of each tag associated sequence read corresponding to a segment of the genomic material to a location on the genome, and counting the number of tagged nucleic acid molecules assigned to the same location on the genome having a different tag, thereby obtaining genomic copy number information unaffected by amplification distortion.. .
03/06/14
20140065603
 Quantitative real-time assay for noroviruses and enteroviruses with built in quality control standard patent thumbnailQuantitative real-time assay for noroviruses and enteroviruses with built in quality control standard
A method is provided for reverse transcription-polymerase chain reaction (rt-pcr) comprising a) amplifying a reverse transcribed cdna in a mixture comprising norovirus genogroup i and norovirus genogroup ii primers and probes, wherein said norovirus primers and probes distinguish between genogroup i and genogroup ii viruses; b) quantifying virus; and c) normalizing data based on a universal internal rna control. Optionally, the method may also include primers and probes for enteroviruses.
03/06/14
20140065602
 Human exhaled aerosol droplet biomarker system and method patent thumbnailHuman exhaled aerosol droplet biomarker system and method
A system and method for detecting a biomarker in exhaled breath condensate nanodroplets comprises noninvasively collecting exhaled breath condensate nanodroplets of a subject, and analyzing said nanodroplets utilizing immuno-quantitative polymerase chain reaction to detect one or more target biomarkers.. .
02/27/14
20140057863
Modified nucleosides for the treatment of viral infections and abnormal cellular proliferation
This invention also provides an effective process to quantify the viral load, and in particular bvdv, hcv or west nile virus load, in a host, using real-time polymerase chain reaction (“rt-pcr”). Additionally, the invention discloses probe molecules that can fluoresce proportionally to the amount of virus present in a sample..
02/27/14
20140057279
Devices and methods for biological sample-to-answer and analysis
Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk.
02/27/14
20140057210
Methods of fabrication of cartridges for biological analysis
Methods to fabricate reaction cartridges for biological sample preparation and analysis are disclosed. A cartridge may have a reaction chamber and openings to allow fluids to enter the chamber.
02/20/14
20140051159
Micro-fluidic modules on a chip for diagnostic applications
A chip having a substrate for amplifying genetic material includes a substrate heater having a plurality of substrate heating resistors. The heating resistors define a first temperature zone for maintaining a first temperature, a second temperature zone for maintaining a second temperature below the first temperature, and a third temperature zone for maintaining a third temperature between the first and second temperatures, on the substrate.
02/20/14
20140051155
Thermal cycler apparatus and related methods
An apparatus for thermal cycling can transfer heat uniformly and efficiently. The apparatus can be used in a method that reduces condensation on sample wells.
02/13/14
20140045250
Real-time optical system for polymerase chain reaction
An improved device and system for facilitating polymerase chain reaction including a light source, detector, waveguide, and filters that occupy minimal space and facilitate reduced sample read time and rapid reading of multiple light wavelengths.. .
02/13/14
20140045190
Method and device for monitoring real-time polymerase chain reaction (pcr) utilizing electro-active hydrolysis probe (e-tag probe)
A method for real-time electrochemical monitoring of pcr amplicons using a hydrolysis probe that is labeled with electro-active indicators and a microchip for implementing the method. The method provided is simpler and has higher specificity compared with the prior art.
02/06/14
20140038896
Retroelements and mental disorders and methods of measuring l1 retrotransposition
A method of treating increased non-ltr retrotransposition in a cell. The method includes exposing a neural cell to a retrotransposition inhibitor in an amount sufficient to decrease the non-ltr retrotransposition in the neural cell or a progeny of the neural cell.
01/30/14
20140031241
Paired end bead amplification and high throughput sequencing
The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a paired end sequencing method that enables the sequencing of unique read pairs by co-localizing both 5′ ends on a single emulsion polymerase chain reaction bead.
01/02/14
20140005073
Method and apparatus for analyzing nucleic acid by compensating for crosstalk in polymerase chain reaction data and other data
A method of analyzing nucleic acid by compensating for crosstalk in polymerase chain reaction (pcr) data and other data, wherein crosstalk signals associated with multiple fluorescent dyes are corrected by using fluorescent intensity variations detected from a concentration difference of the fluorescent dyes, and apparatus for performing the method.. .
12/26/13
20130344539
Non-thermal cycling for polymerase chain reaction
Techniques, systems, and devices are disclosed for non-thermal cycling of polymerase chain reaction (pcr). In one aspect, a method for cycling pcr includes receiving an electrolytic fluid including ions, primers, polymerase enzymes, nucleotides, and a double-stranded nucleic acid in a fluid chamber having a first electrode and a second electrode, applying an electric field across the first and the second electrodes to generate a first ph level of the electrolytic fluid to denature the double-stranded nucleic acid to at least partial single strands, and applying a second electric field across the first and second electrodes to produce a second ph level of the electrolytic fluid, in which the second ph level enables binding of a polymerase enzyme and a primer with a corresponding segment of the single strands..
12/19/13
20130337444
Nano46 genes and methods to predict breast cancer outcome
The present invention provides methods for classifying and for evaluating the prognosis of a subject having breast cancer are provided. The methods include prediction of breast cancer subtype using a supervised algorithm trained to stratify subjects on the basis of breast cancer intrinsic subtype.
12/19/13
20130337434
Method of preparing magnetic bead type nasopharyngeal enzyme immunoassay reagents by polymerase chain reaction
A method of preparing magnetic bead type nasopharyngeal enzyme immunoassay reagents by polymerase chain reaction according to this invention offers in-vitro diagnostic reagents by means of utilizing nanotechnology. This method uses magnetic beads to coat ebv nuclear antigen (epstein-barr virus nuclear antigen, ebna1) or early antigen (early antigen, ea).
11/28/13
20130315886
Retroelements and mental disorders and methods of measuring l1 retrotransposition
A method of treating increased non-ltr retrotransposition in a cell. The method includes exposing a neural cell to a retrotransposition inhibitor in an amount sufficient to decrease the non-ltr retrotransposition in the neural cell or a progeny of the neural cell.
11/21/13
20130310269
Hot-start digital pcr
The present disclosure provides methods and compositions for performing nucleic acid reactions, such as hot-start digital polymerase chain reaction (dpcr).. .
11/21/13
20130309724
Step-up method for cold-pcr enrichment
Methods of using polymerase chain reactions to enrich a target sequence in a sample containing reference sequences and target sequences having high homology and amplifiable by the same primer pair are provided herein. In particular the methods provide a robust means to improve the fold enrichment of the target sequence and minimize reaction-to-reaction, well-to-well and run-to-run variations in the enrichment methods..
11/21/13
20130309684
Nucleic acid-free thermostable enzymes and methods of production thereof
The present invention provides thermostable enzymes, such as dna polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (pcr)..
11/14/13
20130303390
System for integrated analysis of real-time polymerase chain reaction and dna chip and method for integrated analysis using the same
Provided are a system for integrated analysis of a real-time polymerase chain reaction and a dna chip and a method for integrated analysis using the same, and more particularly to an apparatus for integrated analysis of a real-time polymerase chain reaction and a dna chip and a method for integrated analysis using the same. According to the method for integrated analysis of a biomaterial of the present invention, gene amplification proceeds and subsequently hybridization proceeds in a single reactor, thereby preventing contamination of the sample due to external factors, which may be caused while the sample is transferred for reaction, and automating a series of procedures such as injection of the sample, reaction of the biomaterial, and detection and analysis of results..
11/07/13
20130295663
Pressurizable cartridge for polymerase chain reactions
Methods and apparatus for use in connection with the performance of the polymerase chain reaction are provided. An exemplary sample processing module is described that includes a sample assembly and a pcr assembly, the sample processing module being configured to hold the sample therein at a pressure higher than ambient pressure.
10/31/13
20130288238
Methods and compositions for detecting serotypes of chlamydia trachomatis capable of causing lymphogranuloma venereum
Disclosed are methods and compositions for conducting assays utilizing real-time polymerase chain reactions (“pcrs”) in detection of serotypes l i, l ii, and l iii, but not stereotype b, of chlamydia trachomatis, capable of causing lymphogranuloma venereum (“lgv”). These assays take advantage of a deletion occurring in the cytotoxin gene locus specific to the l i, l ii, and l iii serotypes.
10/31/13
20130288229
Method for detection, differentiation and quantification of t cell populations by way of reverse transcription quantitative real time pcr (rt-qpcr) technology
The present invention relates to a method for detection, differentiation and quantification of t cell populations, comprising the following steps a) contacting a first aliquot of a body fluid of an individual with at least one antigen, wherein the body fluid contains antigen presenting cells (apc) and t cells, b) incubating the first aliquot with at least one antigen for a certain period of time, c) detection and differentiation of the t cell population by detecting in the first aliquot and in a second aliquot of the body fluid of the individual, which has not been incubated with the at least one antigen, at least a first marker of the apc induced by t cells in a specific t cell population using reverse transcription quantitative real time-time polymerase chain reaction (rt-qpcr), and d) detection and quantification of the t cell population by determining the ratio of the detected marker of the apc of the first aliquot to the second aliquot as well as a kit for performing the method.. .
10/24/13
20130282294
Methods and systems for processing data
The present invention is directed to methods and systems for applications relating to correction of numerical data resulting from dynamic changes to a true value. Such methods and systems may be used in accurate and unbiased quantitative polymerase chain reaction measurement..
10/17/13
20130274122
Assay for chlamydia trachomatis by amplification and detection of chlamydia trachomatis pmpa gene
A region of the chlamydia trachomatis pmpa gene has been identified which is useful for performing amplification assays to determine specifically whether c. Trachomatis is present in the sample being tested.
10/17/13
20130273535
Ndm-1 polymerase chain reaction (pcr) assay
Provided herein are compositions, methods, and kits for detection, identification, and analysis of ndm-1 variant nucleic acid. In particular, provided herein are kits, compositions, and methods for the detection, identification, and analysis of the ndm-1 variant nucleic acid and bacteria o other organisms carrying the ndm-1 variant nucleic acid..
10/10/13
20130266948
Apparatus and methods for integrated sample preparation, reaction and detection
Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided, as are methods for using such cartridges. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction.
10/03/13
20130259899
Detection of fusobacterium in a gastrointestinal sample to diagnose gastrointestinal cancer
Fusobacterium is a genus of gram-negative, filamentous, anaerobic bacteria found as normal flora in the mouth and large bowel, and often in necrotic tissue. A comparison of microbial ribonucleic acids (rna) between colorectal carcinoma (crc) tissue and adjacent normal control tissue found the over-representation of f.
09/26/13
20130252238
Polymerase chain reaction detection system
The present invention relates to methods and kits for nucleic acid detection in an assay system.. .
09/19/13
20130244286
Compositions and methods for cdna synthesis
Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of rna into cdna and rt pcr without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cdna synthesis or nucleic acid amplification (e.g., by the polymerase chain reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications..
09/12/13
20130237428
Methods for high level multiplexed polymerase chain reactions and homogeneous mass extension reactions
Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid..
09/05/13
20130230848
Oligonucleotides useful in methods for detecting and characterizing aspergillus fumigatus
Methods for using oligonucleotides in the detection of aspergillus fumigatus are disclosed. The oligonucleotides of the invention have nucleotide sequences derived from the gene encoding the cytochrome p450 14 alpha-sterol demethylase (the cyp51a protein) of aspergillus fumigatus.
09/05/13
20130230529
Novel paramyxovirus and uses thereof
Described herein are isolated paramyxovirus, a morbillivirus (fmopv), isolated nucleic acids encoding the genome of fmopv, isolated amino acid sequences of fmopv proteins, antibodies to fmopv and its proteins, and uses thereof. In certain embodiments, the modified fmopv is a feline morbillivirus.
08/29/13
20130225426
Reactivity-dependent and interaction-dependent pcr
Methods, reagents, compositions, and kits for reactivity-dependent polymerase chain reaction (rd-pcr) and interaction-dependent polymerase chain reaction (id-pcr) are provided herein. Rd-pcr is a technique useful for determining whether a reactive moiety can form a covalent bond to a target reactive moiety, for example, in screening a library of candidate reactive moieties for reactivity with a target reactive moiety, and in identifying an enzyme substrate, for example, in protease substrate profiling.
08/29/13
20130224746
Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (pcr) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence.
08/22/13
20130217112
Reaction tube for performing isothermal polymerase chain reaction therein
A reaction tube for performing isothermal polymerase chain reaction therein is provided and includes an upper section, a lower capillary section, a linkage connecting the upper section and the lower capillary section, and a thermal conductor. The lower capillary section has an annealing portion, an annular heating groove, and a close end.
08/22/13
20130217071
Methods and compositions for performing nucleic acid amplification reactions
The present disclosure provides methods and compositions for performing nucleic acid reactions, such as the reverse transcriptase-polymerase chain reaction (rt-pcr).. .
08/22/13
20130217025
Chimeric dna identifier
Devices and methods for delivering a chimeric deoxyribonucleic acid (dna) marking agent to a target and identifying the target from the chimeric dna marking agent are provided. A delivery device may be a projectile, a spray canister or a wet/dry article.
08/15/13
20130210645
Personalized tumor biomarkers
Clinical management of human cancer is dependent on the accurate monitoring of residual and recurrent tumors. We have developed a method, called personalized analysis of rearranged ends (pare), which can identify translocations in solid tumors.
08/15/13
20130210080
Pc board-based polymerase chain reaction systems, methods and materials
An apparatus for performing a polymerase chain reaction (pcr) is disclosed. The apparatus comprises a pcr chamber for performing a polymerase chain reaction and a printed circuit board (pcb) fluidic device.
08/15/13
20130210005
Method for detecting mycobacterium tuberculosis and nontuberculous mycobacteria by using dual real-time polymerase chain reaction
Disclosed are a primer set and/or a probe capable of detecting specific nucleotide sequences of mtc and ntm, a kit for the detection of mtc and ntm, comprising the same, and a method for detecting mtc and ntm by duplex real-time pcr using the same. Useful in detecting genes characteristic of mtc and ntm, the primer sets and/or probes, detection kits, and detection methods can be applied as the clinical diagnosis of diseases caused by mtc and ntm, and therefore find applications in the medical fields including hospitals, research institutes, etc..
08/08/13
20130203120
Method for cell lysis and pcr within the same reaction chamber
A method for amplification of a target dna, comprising the steps of (i) transferring a liquid with a first volume comprising at least one or more living cells into a vessel (ii) adding to said vessel a pcr reaction buffer with a second volume, whereas said second volume is at least 2× as large as said first volume (iii) lysing said at least one or more living cells within said vessel by means of incubation for at least 1 minute at at least 90° c., and (iv) amplifying said target by means of a polymerase chain reaction without performance of an intermediate purification step.. .
08/01/13
20130196939
Detection of dna sequences as risk factors for hiv infection
A method for identifying a risk factor for diseases, disorders or conditions, such as those caused by human immunodeficiency virus, using the polymerase chain reaction and specific primers. Methods for treating patients having these diseases, disorders or conditions by antimicrobial treatment of the risk factor by combined antiviral and antibacterial treatment or by sustaining or stimulating the subject's immune system.
08/01/13
20130196318
Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
The present invention relates generally to the field of detection of microorganisms, in particular detection of bacteria, to methods for measuring enzyme activity, such as dna polymerase activity, and particularly relates to such methods performed on microbial crude lysates, useful for determining microbial enzyme activities which can be linked to amplification signal generators such as real-time polymerase chain reaction (pcr) techniques, thereby enabling determination of microbial pathogens in samples such as unpurified blood and other body fluids. This invention also relates to reagents for use in such methods, and to test kits comprising such reagents for carrying out the methods..
08/01/13
20130195139
Sample container with sensor receptacle and methods of use
Devices and methods are described herein that are configured for use in laboratory testing, such as, for example, during a procedure including the monitoring and detection of chemical reactions. For example, the systems and devices described herein can be used during a procedure to monitor and detect polymerase chain reactions (pcr).
07/18/13
20130184176
Methods and compositions for rapid multiplex amplification of str loci
Provided are methods for multiplex polymerase chain reaction (pcr) amplification of short tandem repeat (str) loci that can be used to rapidly generate a highly specific str profile from target nucleic acids. The resulting str profiles are useful for human identification purposes in law enforcement, homeland security, military, intelligence, and paternity testing applications..
07/18/13
20130183717
Channels with cross-sectional thermal gradients
Provided herein are systems, devices, and methods for generating thermal gradients in channels and uses thereof. In particular, provided herein are system, methods, and devices employing first and second thermal layers positioned around a channel in order to create a thermal gradient across a cross-section of the channel having, for example, a nucleic acid denaturation zone, a nucleic acid annealing zone, and a nucleic acid polymerization zone.
07/11/13
20130177971
Non-contact heating type of gene amplification system
Disclosed is a non-contact heating type gene amplification system. The non-contact heating type gene amplification system includes separate light sources for each step of a polymerase chain reaction in order to perform a high-speed temperature cycle by moving a rotation unit, in which a plurality of sample chambers are formed, to places at which the sample chambers are controlled at the temperatures corresponding to each step..
07/11/13
20130177914
Method for detecting mycobacterium tuberculosis and nontuberculous mycobacteria using duplex polymerase chain reaction
Disclosed are primer sets specific for the is6110 or 16s rrna gene characteristic of mtc and for the 16s rrna gene characteristic of ntm, kits for the detection of mtc and ntm, comprising the same, and methods for detecting mtc and ntm by duplex pcr using the same. Because the primers are exclusive to mycobacterium tuberculosis and nontuberculous mycobacteria, the methods, which can employ an inexpensive typical pcr cycler, can be a clinical diagnostic means useful for the detection of both mycobacterium tuberculosis and nontuberculous mycobacteria at the same time, with higher efficiency..
07/11/13
20130177913
Real-time pcr in micro-channels
The present invention relates to methods for amplifying nucleic acids in micro-channels. More specifically, the present invention relates to methods for performing a real-time polymerase chain reaction (pcr) in a continuous-flow microfluidic system and to methods for monitoring real-time pcr in such systems..
06/27/13
20130164788
Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)
The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (rt-pcr). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step rt-pcr procedure using combinations of reverse transcriptase and thermostable dna polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin.
06/27/13
20130164756
Method for detecting mycobacterium tuberculosis and nontuberculous mycobacteria using duplex real-time polymerase chain reaction and melting curve analysis
Disclosed are primer sets specific for the is6110 gene characteristic of mtc and for the 16s rrna gene characteristic of ntm, kits for the detection of mtc and ntm, comprising the same, and methods for detecting mtc and ntm by duplex real-time pcr and melting profile analysis using the same. Because the primers are exclusive to mycobacterium tuberculosis and nontuberculous mycobacteria, the methods can be a clinical diagnostic means useful for the detection of both mycobacterium tuberculosis and nontuberculous mycobacteria at the same time, with higher efficiency..
06/13/13
20130149710
Microdroplet-manipulation systems and methods for automated execution of molecular biological protocols
Disclosed herein are automated systems for performing various biochemical and molecular biological procedures, including processor-controlled execution of protocols involving multiple steps performed in, on, or with liquid microdroplets. Example protocols are the various polymerase chain reaction (pcr) protocols, but the subject systems are not limited to performing pcr protocols.
06/13/13
20130149698
Stable compositions for nucleic acid amplification and sequencing
The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable dna polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing.
06/13/13
20130146462
Control of chemical reactions using isotachophoresis
Isotachophoresis (itp) is exploited to control various aspects of chemical reactions. In a first aspect, at least one of the reactants of a chemical reaction is confined to an itp zone, but the resulting product of the chemical reaction is separated from this itp zone by the itp process.
06/06/13
20130143746
Method for detecting gene region features based on inter-alu polymerase chain reaction
An array of inter-alu gene-enriched amplicons produced by a polymerase chain reaction (“pcr”) process. The pcr process comprises combining one or a plurality of head-type/tail-type alu- or aluy- or any other alu-subfamily-consensus sequence-based primer; a genomic dna template isolated from cells; and a pcr-extension mix.
06/06/13
20130143205
Highly sensitive method for detection of viral hiv dna remaining after antiretroviral therapy of aids patients
Methods for detecting polynucleotides, especially the dna replicated from samples obtained from subjects infected with pathogenic viruses such as human immunodefiency virus, by detecting electromagnetic signals (“ems”) emitted by such polynucleotides, and methods for improving the sensitivity of the polymerase chain reaction (“pcr”).. .
05/30/13
20130137594
Systems and methods for performing amplicon rescue multiplex polymerase chain reaction (pcr)
Embodiments of the present disclosure generally pertain to systems and methods for performing amplicon rescue multiplex polymerase chain reaction (arm-pcr). In one embodiment, the system comprises a processor and a reader coupled to a control element.
05/30/13
20130137105
Thermal cycling using phase changing fluids
The invention provides systems, devices, and methods for heating and cooling chemical or biological samples, such as genetic materials during polymerase chain reaction (“pcr”). The systems, devices, and methods comprise use of a fluid that performs repeated heating and cooling cycles, e.g., ‘thermal cycling’, on sample reactants with a phase changing fluid during evaporation and condensation.
05/16/13
20130122552
Low-mass sample block with rapid response to temperature change
A sample block for use in the polymerase chain reaction, dna sequencing, and other procedures that involve the performance of simultaneous reactions in multiple samples with temperature control by heating or cooling elements contacting the bottom surface of the block is improved by the inclusion of hollows in the block that are positioned to decrease the mass of the block in the immediate vicinity of the wells.. .
05/09/13
20130115614
Method for detecting and quantifying endogenous wheat dna sequence
A primer pair is provided capable of amplifying partial sequences of endogenous wheat dna which are single copies and which allow wheat to be specifically detected without cross-reacting with other plants in a polymerase chain reaction. Also provided is a kit for detecting or assaying an endogenous wheat dna sequence in a test sample by a polymerase chain reaction..
05/09/13
20130115611
Systems and methods for calibration using dye signal amplification
The present teachings relate to a method of generating calibration information during a real-time polymerase chain reaction (rt-pcr) or other amplification reaction. A sample well plate or other support can contain one or more dyes or other reference materials that are subjected to the same rt-pcr thermal cycles or other conditions used to conduct amplification or other reactions on a biological sample.


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Polymerase Chain Reaction topics: Polymerase Chain Reaction, Polymerase, Nucleic Acid, Nucleotide, Quantitative, Amplification, Chlamydia Trachomatis, Nucleic Acids, Transcription, Oligonucleotide, Sequencing, Real Time Pcr, Differentiation, Lymphogranuloma Venereum, Recombinant

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