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Polymerase Chain Reaction

Polymerase Chain Reaction-related patent applications - as published by the U.S. Patent and Trademark Office (USPTO).


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Porous matrix comprising nucleic acid primer-carbon material composites and pcr using the same
Korea Institute Of Science And Technology
November 09, 2017 - N°20170321265

A porous matrix according to the present disclosure, wherein a nucleic acid primer-carbon material composite in which one or more nucleic acid primer of a forward primer and a reverse primer as a polymerase chain reaction (pcr) primer is bound to a carbon material is included in the pores of the matrix, provides improved amplification efficiency as compared to a ...
Methods and reagents for reverse-transcription polymerase chain reaction
Ge Healthcare Uk Limited
November 09, 2017 - N°20170321250

The present invention provides a method of amplifying an rna molecule in a biological sample by reverse transcription pcr (rt-pcr), wherein the rt-pcr is carried out in a solution comprising a polar aprotic solvent; a serum albumin, and a polyol.
Methods for culturing and analyzing cells
Stokes Bio Limited
November 09, 2017 - N°20170320025

A system for analyzing a biological sample may include at least one sample acquisition stage comprising a sample acquisition device for acquiring the biological sample from a sample source; a droplet generator device for forming a droplet wrapped in an immiscible carrier fluid, wherein the wrapped droplet comprises at least the biological sample and a reagent, the droplet generator configured ...
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Interfacial effects enable droplet actuation, inhibition relief, and early sensing of polymerase chain reaction
Streck, Inc.
October 19, 2017 - N°20170298416

Disclosed are devices, systems and methods of use utilizing interfacial effects enabling droplet actuation, inhibition and early sensing of molecular reactions, such as of polymerase chain reaction.
Binding and catalytic molecules built from l-dna with added nucleotides
Streck, Inc.
October 19, 2017 - N°20170298356

This invention provides for processes for binding to and/or chemically transforming a preselected target, where the process involves contacting said target to an oligonucleotide molecule that contains one or more “non-standard” nucleotides, which are nucleotide analogs that, when incorporated into oligonucleotides (dna or rna, collectively xna), present to a pattern of hydrogen bonds that is ...
Qualitative and quantitative detection of microbial nucleic acids
Roche Molecular Systems, Inc.
October 12, 2017 - N°20170292145

The present invention relates to new methods and uses for the qualitative and quantitative detection of microbial nucleic acids using at least a first control nucleic acid, or a first and a second control nucleic acid in different concentrations. The method is based on amplification of nucleic acids, for example the polymerase chain reaction. Further provided are kits comprising components ...
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Devices and methods for molecular diagnostic testing
Click Diagnostics, Inc.
September 14, 2017 - N°20170259263

A hand-held molecular diagnostic test device includes a housing, an amplification (or pcr) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (pcr) on the input sample. The detection module is configured ...
Polymerase chain reaction primers and probes for mycobacterium tuberculosis
Rutgers, The State University Of New Jersey
August 31, 2017 - N°20170247747

The present invention relates to novel primers and sloppy molecular beacon and molecular beacon probes for amplifying segments from different genes in mycobacterium tuberculosis for identifying the presence of m. Tb dna and/or resistance to anti-tuberculosis drugs.
Two-stage thermal convection apparatus and uses thereof
Ahram Biosystems, Inc.
August 24, 2017 - N°20170239662

Disclosed is a multi-stage thermal convection apparatus such as a two-stage thermal convection apparatus and uses thereof. In one embodiment, the two-stage thermal convection apparatus includes a temperature shaping element that assists a thermal convection mediated polymerase chain reaction (pcr). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many ...
Three-stage thermal convection apparatus and uses thereof
Ahram Biosystems, Inc.
August 24, 2017 - N°20170239654

Disclosed is a multi-stage thermal convection apparatus and uses thereof. In one embodiment, the invention features a three-stage thermal convection apparatus that includes a temperature shaping element for assisting a thermal convection mediated polymerase chain reaction (pcr). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In ...
Tritrichomonas foetus nucleic acid detection methods
The Texas A&m University System
August 17, 2017 - N°20170233830

The present invention provides quantitative polymerase chain reaction (pcr) methods and kits for diagnosing trichomoniasis.
Borrellia provocation procedure protocol
The Texas A&m University System
August 17, 2017 - N°20170233793

The testing for the lyme disease pathogen, (borrelia bergdorferi (bb) and all other lyme borrelia), is notoriously difficult. Bb is not by nature a blood loving bacteria, and prefers the climate of avascular tissues such as cartilage. The borrelia provocation procedure disclosed herein proposes to uniquely lure the lyme borrelia spirochetes into the blood through a simple procedure of sub-dermal ...
Immunoglobulin variable region libraries
Xbiotech, Inc.
August 17, 2017 - N°20170233457

Antigen-specific immunoglobulin v-regions are identified from a library of nucleic acids amplified using polymerase chain reaction using leader sequence-specific forward primers. The use of leader sequence primers allows all v-region sequences to be amplified (including those with extensive 5′ end mutations) without loss of the original 5′ v gene segment sequence. These libraries can be screened for antigen-specific v-regions using ...
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Thermal uniformity for thermal cycler instrumentation using dynamic control
Life Technologies Corporation
August 10, 2017 - N°20170225169

A method for performing polymerase chain reactions (pcr) for improving thermal non-uniformity is provided. The method includes measuring a first temperature, by a first sensor, of a first sample block sector of a sample block and measuring a second temperature, by a second sensor, of a second sample block sector of the sample block that is adjacent to the first ...
In vitro method for the detection and quantification of hiv-2
Universite Paris Diderot
August 03, 2017 - N°20170218467

The present invention relates to a method for detecting or quantifying human immunodeficiency virus-2 (hiv-2) nucleic acids in a biological sample, comprising: a) performing a real-time polymerase chain reaction (pcr) or a real-time reverse transcriptase polymerase chain reaction (rt-pcr) on nucleic acids of the biological sample with: (i) at least 4 primers respectively comprising or consisting of: —sequence seq id ...
Random access system and method for polymerase chain reaction testing
Siemens Healthcare Diagnostics Inc.
August 03, 2017 - N°20170218432

A random access, high-throughput system and method for preparing a biological sample for polymerase chain reaction (pcr) testing are disclosed. The system includes a nucleic acid isolation/purification apparatus and a pcr apparatus. The nucleic acid isolation/purification apparatus magnetically captures nucleic acid (na) solids from the biological sample and then suspends the na in elution buffer solution. The pcr ...
Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ...
Cornell University
July 27, 2017 - N°20170212103

The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and ...
Quantitative reverse transcription polymerase chain reaction kit for breast cancer drug screening test and early ...
University Industry Foundation, Yonsei University Wonju Campus
July 27, 2017 - N°20170211156

There are provided an information offering method for diagnosing breast cancer comprising: (a) separating total rna from a cell obtained from a tissue or blood of a suspected cancer patient; (b) synthesizing a cdna from the separated total rna; (c) performing a real-time pcr with the synthesized cdna by using primer pair mix and probe mix that can amplify human ...
Process and molecular biomarker
Centro De Hemoterapia E Hematologia Do Para - Hemopa
July 20, 2017 - N°20170204464

A process and molecular biomarker for platelets stored in blood banks provides for efficient verification of which platelet concentrate bags are in good condition for transfusion by measuring, by real-time polymerase chain reaction, expression levels of hsa-mir-127 and hsa-mir-320a micrornas as the first process and biomarker predictor of platelet's cell aging during storage and the consequent presence of storage ...
Method for the detection of sepsis
Centro De Hemoterapia E Hematologia Do Para - Hemopa
July 20, 2017 - N°20170204452

The invention relates to an in vitro method for the detection and/or identification of a human pathogen and/or genetic material thereof comprising subjecting a sample comprising or suspected of comprising a human pathogen and/or genetic material thereof to a multiplex real-time polymerase chain reaction (rt-pcr), wherein said method comprises amplification of pcr products that enable discrimination between ...
Devices and methods for molecular diagnostic testing
Click Diagnostics, Inc.
July 20, 2017 - N°20170203297

A hand-held molecular diagnostic test device includes a housing, an amplification (or pcr) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (pcr) on the input sample. The detection module is configured ...
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