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Polymerase Chain Reaction patents



      

This page is updated frequently with new Polymerase Chain Reaction-related patent applications.




Date/App# patent app List of recent Polymerase Chain Reaction-related patents
05/19/16
20160138083 
 Determination of variants produced upon replication or transcription of nucleic acid sequences patent thumbnailnew patent Determination of variants produced upon replication or transcription of nucleic acid sequences
A method of determining whether or not a nucleic acid having an expected sequence or one or more variants of the expected sequence are present in a sample containing nucleic acids after replication, transcription or editing (or other transformation) of a substrate nucleic acid. The method involves deciding an expected sequence likely to be formed in the sample upon the replication, transcription or editing of the substrate nucleic acid, and possible variants of the expected sequence, providing primer pairs for a polymerase chain reaction, reverse transcriptase polymerase chain reaction or ligase chain reaction, carrying out the polymerase chain reaction or reverse transcriptase polymerase chain reaction in one or more steps to form amplicons, and analyzing the amplicons to determining whether or not a nucleic acid having the expected sequence and/or variants are present in the sample.
Bio-id Diagnostic Inc.


05/12/16
20160130662 
 Novel genomic marker of metastatic prostate cancer patent thumbnailNovel genomic marker of metastatic prostate cancer
The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for assessing prostate cancer in a patient.
The Johns Hopkins University


04/28/16
20160115530 
 Determination of methylation state and chromatin structure of target genetic loci patent thumbnailDetermination of methylation state and chromatin structure of target genetic loci
The subject invention pertains to a method of determining methylation state and chromatin structure of target loci. The method comprises treating the genetic material obtained from the cells with dna methyltransferase, capturing target genetic loci using a set of oligonucleotides, ligating the target loci with oligonucleotide patches that flank the target loci, treating the target loci flanked by oligonucleotide patches with bisulfite, optionally amplifying the target loci by polymerase chain reaction, sequencing the pcr products, and analyzing the sequences to determine methylation state and chromatin structure of the target loci.
University Of Florida Research Foundation, Inc.


04/14/16
20160103952 
 System and methods for determination of temperature cycling protocols for polymerase chain reactions patent thumbnailSystem and methods for determination of temperature cycling protocols for polymerase chain reactions
In one aspect, methods are described herein for enhancing one or more nucleic acid interactions. For example, in some embodiments, methods of enhancing one or more steps of polymerase chain reaction (pcr) are described herein.

04/14/16
20160102347 
 Methods for high level multiplexed polymerase chain reactions and homogeneous mass extension reactions patent thumbnailMethods for high level multiplexed polymerase chain reactions and homogeneous mass extension reactions
Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid..

03/24/16
20160083786 
 In situ interaction determination patent thumbnailIn situ interaction determination
Methods, reagents, compositions, and kits for in situ interaction determination (isid) via interaction-dependent polymerase chain reaction (id-pcr) are provided herein. Isid technology is useful for rapidly evaluating potential small molecule-target interactions from mixtures in a single solution.
President And Fellows Of Harvard College


03/24/16
20160083778 
 Application of thiolated single-stranded dna in polymerase chain reaction patent thumbnailApplication of thiolated single-stranded dna in polymerase chain reaction
An application of thiolated single-stranded dna enhances specific amplification of polymerase chain reaction, namely a method for enhancing specific amplification of polymerase chain reaction by utilizing thiolated single-stranded dna, which includes the following step: adding an appropriate amount of the thiolated single-stranded dna into a pcr system to perform pcr amplification, wherein the appropriate amount means that the final concentration of the thiolated single-stranded dna in a 20 μl reaction system is not less than 15 μm. The thiolated single-stranded dna meets the following conditions: the thiolated single-stranded dna is one segment of any sequence which is non-complementary and non-homologous to a target sequence; the tm value is not less than 37.7° c.; and at least one end contains a thiolalkyl group sh—c6h12—..
Institute Of Plant Protection, Shandong Academy Of Agricultural Sciences


03/17/16
20160076110 
 Methods for pathogen detection and disease management on meats, plants, or plant parts patent thumbnailMethods for pathogen detection and disease management on meats, plants, or plant parts
Provided are methods for detecting pathogens affecting meats, plants, or plant parts. Also provided are methods for predicting disease and/or disease management for meats, plants, or plant parts.
Agrofresh Inc.


03/03/16
20160061731 
 Analysis  analyzing a nucleic acid amplification reaction patent thumbnailAnalysis analyzing a nucleic acid amplification reaction
An analysis method is provided aimed at improving the linearity (precision and/or accuracy) of a quantification by a real-time nucleic acid amplification reaction rnr such as a polymerase chain reaction pcr over a wide range of analyte concentrations and/or limiting the effects of the presence of interfering substances by way of determining a quantification cycle number (cq) of the rnr as the cycle number corresponding to an intersection of the growth curve with a combined threshold function (ctf) over the rnr cycle range comprising at least two different threshold levels, the quantification cycle number (cq) being indicative of a quantitative and/or qualitative analysis result of a growth curve, the growth curve being indicative of the intensity of the fluorescence emission of an analyte for each amplification reaction cycle of the rnr over a rnr cycle range.. .
Roche Molecular Systems, Inc.


03/03/16
20160060672 
 Method and device for polymerase chain reaction patent thumbnailMethod and device for polymerase chain reaction
Method and apparatus for amplifying a target nucleic acid sequence of a reaction mixture in a polymerase chain reaction (pcr). The method includes contacting the reaction mixture with emr frequency absorbing particles formed from a material having a transition metal, transition metal oxide or a transition metal hydroxide, or a nitride, a phosphide or an arsenide of a group iii metal doped with the transition metal or a transition metal oxide, or silicon dioxide doped with the transition metal, transition metal oxide, or transition metal hydroxide; and irradiating the emr absorbing particles with emr having a frequency of about 200 khz to 500 thz to amplify the target nucleic acid sequence, wherein the group iii metal is any one of al, ga, and in, and the transition metal is any one of mn, fe, co and cu..
National Cheng Kung University


03/03/16
20160060619 

Identifying affinity-matured human antibodies


Antigen-specific immunoglobulin v-regions are identified from a library of nucleic acids amplified using polymerase chain reaction using leader sequence-specific forward primers. The use of leader sequence primers allows all v-region sequences to be amplified (including those with extensive 5′ end mutations) without loss of the original 5′ v gene segment sequence.
Xbiotech, Inc.


03/03/16
20160060618 

Identifying affinity-matured human antibodies


Antigen-specific immunoglobulin v-regions are identified from a library of nucleic acids amplified using polymerase chain reaction using leader sequence-specific forward primers. The use of leader sequence primers allows all v-region sequences to be amplified (including those with extensive 5′ end mutations) without loss of the original 5′ v gene segment sequence.
Xbiotech, Inc.


02/25/16
20160051985 

System and serial processing of multiple nucleic acid assays


The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (pcr) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided.
Canon U.s. Life Sciences, Inc.


02/18/16
20160046975 

Compositions and methods for cdna synthesis


Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of rna into cdna and rt pcr without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cdna synthesis or nucleic acid amplification (e.g., by the polymerase chain reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications..

02/11/16
20160040222 

Systems and methods for baseline correction using non-linear normalization


Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (pcr), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction.
Applied Biosystems, Llc


02/04/16
20160032357 

Method for relative quantification of changes in dna methylation, using combined nuclease, ligation, and polymerase reactions


The present invention is directed to methods for identifying the presence of one or more methylated or unmethylated target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction.
Cornell University


01/28/16
20160021882 

Application of biofilm formation inhibiting compounds enhances control of citrus canker


Citrus canker caused by the bacterium xanthomonas citri subsp. Citri (xac) is an economically important disease of citrus worldwide.
University Of Florida Research Foundation, Inc.


01/21/16
20160017425 

Detection of genomic rearrangements by sequence capture


Provided herein is a method of sample analysis. In some embodiments, the method comprises hybridizing fragmented genomic dna from a test genome with a population of first oligonucleotides of the formula v1-b-v2 in the presence of one or more second oligonucleotides; contacting the product with ligase to join the ends of the fragmented genomic dna that are hybridized to v1 and v2 to the one or more second oligonucleotides; and subjecting the product to polymerase chain reaction conditions using amplification primers that hybridize to sites that are provided by the one or more second oligonucleotides, wherein production of a product indicates that the test genome contains a chromosomal rearrangement relative to the reference genome..
Agilent Technologies, Inc.


01/21/16
20160017315 

Methods for one step nucleic acid amplification of non-eluted samples


The present invention relates to methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. For easy processing and amplification of nucleic acid samples, the samples are bound to a solid support and used directly, without purification, in a nucleic acid amplification reaction such as the polymerase chain reaction (pcr)..
Ge Healthcare Uk Limited


01/07/16
20160002712 

Polymerase chain reaction detection system


The present invention relates to methods and kits for nucleic acid detection in an assay system.. .
Lgc Genomics Ltd


12/31/15
20150376724 

Detection of dna sequences as risk factors for hiv infection


A method for identifying a risk factor for diseases, disorders or conditions, such as those caused by human immunodeficiency virus, using the polymerase chain reaction and specific primers. Methods for treating patients having these diseases, disorders or conditions by antimicrobial treatment of the risk factor by combined antiviral and antibacterial treatment or by sustaining or stimulating the subject's immune system.

12/31/15
20150376721 

Direct quantitative pcr absent minor groove binders


Disclosed herein are methods, compositions and kits for the quantification of a nucleic acid target present on a solid support. This entails quantitative real-time polymerase chain reaction wherein minor groove binders are excluded..
Life Technologies Corporation


12/31/15
20150376687 

Direct quantitative pcr absent minor groove binders


Disclosed herein are methods, compositions and kits for the quantification of a nucleic acid target present on a solid support. This entails quantitative real-time polymerase chain reaction wherein minor groove binders are excluded..
Life Technologies Corporation


12/31/15
20150376671 

Compositions and methods for inhibiting terminal transferase activity


The present invention realtes to systems and methods for amplifying nucleic acid. In particular, systems and methods are provided for inhibiting polymerase based terminal transferase activity within a polynucleotide amplification setting (e.g., polymerase chain reaction).
Ibis Biosciences, Inc.


12/24/15
20150368702 

Systems and methods for calibration using dye signal amplification


The present teachings relate to a method of generating calibration information during a real-time polymerase chain reaction (rt-pcr) or other amplification reaction. A sample well plate or other support can contain one or more dyes or other reference materials that are subjected to the same rt-pcr thermal cycles or other conditions used to conduct amplification or other reactions on a biological sample.
Applied Biosystems, Llc


12/24/15
20150368696 

Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms


A method for amplifying and detecting microorganisms, such as species of listeria, is described. The method utilizes gene-matched enrichment media and pcr-based detection.
Battelle Memorial Institute


12/17/15
20150361511 

Freeze-dried composition


The invention relates to the use of a polysaccharide having at least four saccharide units, such as stachyose, as a glass-forming agent for the freeze-drying of a reaction mixture comprising an enzyme. In particular, the enzyme is a polymerase useful in a nucleic acid amplification reaction such as a polymerase chain reaction.
Fluorogenics Ltd


12/10/15
20150353999 

Assay and other reactions involving droplets


The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel.
President And Fellows Of Harvard College


12/10/15
20150353988 

Borrelia provocation procedure kit


The testing for the lyme disease pathogen, (borrelia bergdorferi (bb) and all other lyme borrelia), is notoriously difficult. Bb is not by nature a blood loving bacteria, and prefers the climate of avascular tissues such as cartilage.

12/03/15
20150344970 

Personalized tumor biomarkers


Clinical management of human cancer is dependent on the accurate monitoring of residual and recurrent tumors. We have developed a method, called personalized analysis of rearranged ends (pare), which can identify translocations in solid tumors.
The Johns Hopkins University


11/26/15
20150337365 

Polymerase chain reaction detection system


The present invention relates to methods and kits for nucleic acid detection in an assay system.. .
Lgc Genomics Limited


11/12/15
20150322488 

Instrument for monitoring polymerase chain reaction of dna


An optical instrument monitors pcr replication of dna in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded dna. A beam splitter passes an excitation beam to the vials to fluoresce the dye.
Life Technologies Corporation


11/12/15
20150320998 

Device, killing viruses in blood


A device, system and method for killing viruses in blood. An iontophoretic terminal delivery system includes a smart catheter, aleated electrode within the smart catheter, a terminal and an electrical system.

10/29/15
20150307944 

Kit comprising primers for amplifying alk kinase domain nucleic acids


Disclosed are methods and kits for detecting the presence of a cancer in a subject and assessing the efficacy of treatments for the same. The disclosed method use reverse transcription polymerase chain reaction (rt-pcr) techniques to detect the presence of point mutations, truncations, or fusions of anaplastic lymphoma kinase..
Insight Genetics, Inc.


10/29/15
20150307910 

Methods and systems for fast pcr heating


A microplate for polymerase chain reaction (pcr) comprises a substrate having a metallic material for heating pcr samples, and a barrier layer disposed adjacent to the substrate. In some cases, the barrier layer is formed of a first polymeric material.
Bjs Ip Ltd.


10/22/15
20150299794 

Quantitative reverse transcription polymerase chain reaction kit for breast cancer drug screening test and early diagnosis using tissue and blood


A method for a breast cancer drug screening including separating a full-length rna from a cell obtained from a tissue or blood of a suspected cancer patient; synthesizing a cdna from the full-length rna; performing a pcr with the synthesized cdna by using a composition including sets of a pair of primers and a probe, each set independently able to amplify human epidermal growth factor receptor 2 (her2), cytokeratin 19, epcam, a htert, ki67, vimentin, and a gapdh. The method further includes comparing an amount of the amplification with an expression amount to a normal person.
Yonsei University Wonju Industry-academic Cooperation Foundation


10/22/15
20150299778 

Primers, probes, and methods for mycobacterium tuberculosis specific diagnosis


Practical and rapid methods for the diagnosis of mycobacterium tuberculosis (mtb) using the polymerase chain reaction (pcr) have long been sought particularly because of the incidence of human immunodeficiency virus (hiv) coinfection and because standard culture methods are not practical given the long incubation periods needed and the growing prevalence of drug resistant strains.. .
Boston Medical Center Corporation


10/22/15
20150299774 

Method for determining the presence of diarrhoea causing pathogens


This invention relates to the field of detection of diarrhoea causing pathogens from patient, food or environmental samples. Particularly, the present invention provides a polymerase chain reaction (pcr) based assay method for detection of diarrhoea causing pathogens.
Mobidiag Oy


10/22/15
20150299770 

Methods for one step nucleic acid amplification of non-eluted samples


The present invention relates to methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. For easy processing and amplification of nucleic acid samples, the samples are bound to a solid support and used directly, without purification, in a nucleic acid amplification reaction such as the polymerase chain reaction (pcr)..
Ge Healthcare Uk Limited


10/15/15
20150292003 

Methods and kits for detection of a pathogen in sugarcane


Embodiments of the present invention provide a diagnostic approach utilizing quantitative polymerase chain reaction (pcr) to detect quantitatively a pathogen of the genus leifsonia that causes ratoon stunting disease (rsd) in sugarcane. This is a rapid, cost-effective and/or high sensitivity methodology for detecting this pathogen.

10/08/15
20150283545 

Reaction plate


A reaction plate includes a welded plastics planar laminate consisting of an aperture plate and a film, the aperture plate having at least one planar surface and a plurality of apertures in the planar surface of the apertured plate and the film being attached to the planar surface of the apertured plate around the or each aperture by welding. The welding is preferably laser or transmission welding.

10/01/15
20150275294 

Nucleic acid amplification controls and kits and methods of use thereof


The invention provides components and methods for polymerase chain reaction assays. The assays minimize both handling of material and time spent running samples.
Idexx Laboratories, Inc.


10/01/15
20150275276 

Pcr reaction mixtures and methods of using same


Provided herein are methods and compositions involving polymerase chain reaction (pcr).. .
Molecular Detection Israel Ltd.


09/24/15
20150267252 

Pcr assay for animal origin of heparin


The invention provides methods and reagents for use in distinguishing heparin sodium of porcine origin from heparin sodium of bovine origin using quantitative polymerase chain reaction.. .

09/17/15
20150258548 

Apparatus and methods for integrated sample preparation, reaction and detection


Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided, as are methods for using such cartridges. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction.
Luminex Corporation


09/10/15
20150253201 

Sample container with sensor receptable and methods of use


Devices and methods are described herein that are configured for use in laboratory testing, such as, for example, during a procedure including the monitoring and detection of chemical reactions. For example, the systems and devices described herein can be used during a procedure to monitor and detect polymerase chain reactions (pcr).
Heatflow Technologies Inc.


08/27/15
20150238967 

Methods, systems, and applications for solar-thermal microfluidic pcr


Disclosed are methods and apparatus for solar-thermal microfluidic polymerase chain reaction. A device comprises a microfluidic chip including at least one pcr region, an energy absorption layer disposed adjacent to the microfluidic chip, a solar energy concentrator adapted to produce a plurality of temperature profiles on the microfluidic chip adapted to facilitate pcr, and a photomask disposed between the solar energy concentrator and the microfluidic chip..
Cornell University


08/20/15
20150232940 

Methods and compositions relating to diagnosing and treating receptor tyrosine kinase related cancers


Disclosed are methods and compositions for detecting the presence of a cancer in a subject and assessing the efficacy of treatments for the same. The disclosed method use reverse transcription polymerase chain reaction (rt-pcr), real time polymerase chain reaction, and multiplex polymerase chain reaction techniques to detect fusions, over-expression, truncation, and nucleic acid variation of ret and depdc1 in cancers..
Insight Genetics, Inc


08/13/15
20150224505 

Method and use in temperature controlled processing of microfluidic samples


Embodiments of the invention comprise microfluidic devices, instrumentation interfacing with those devices, processes for fabricating that device, and methods of employing that device to perform pcr amplification. Embodiments of the invention are also compatible with quantitative polymerase chain reaction (“qpcr”) processes.
Caliper Life Sciences, Inc.


08/06/15
20150218657 

Real-time pcr assay for detection of babesia microti and clinical utilization in diagnosis and treatment of babesiosis


Methods and kits are provided for detecting babesia microti using real-time polymerase chain reaction.. .
Westchester Medical Center Advanced Physician Services, Pc


07/30/15
20150209789 

Sample processing apparatus and automatic analyzing apparatus including the same


A sample processing apparatus is for extracting a nucleic acid from a sample and amplifying the nucleic acid, wherein the sample processing apparatus including a housing including a chamber; a valve positioned below the housing; and a polymerase chain reaction (pcr) portion positioned below the valve, wherein the polymerase chain reaction portion performing a polymerase chain reaction.. .
Infopia Co., Ltd.


07/16/15
20150197788 

Method for multiplexed nucleic acid patch polymerase chain reaction


The invention encompasses a method for amplifying at least two different nucleic acid sequences. In particular, the method encompasses a multiplexed nucleic acid patch polymerase chain reaction..
Washington University


07/02/15
20150184225 

Electrokinetic polymerase chain reaction (pcr) devices and methods


Described herein are microfluidic diagnostic methods and devices using electrokinetic modules for the isolation of targets (e.g., cells, bacteria, biomolecules) from biological samples, pcr amplification of dna isolated from the targets, and real-time quantification of the amplified dna using impedance sensing. Sample preparation, pcr amplification, and impedance sensing are thus performed using a single integrated platform..
Genefluidics, Inc.


07/02/15
20150184151 

Methods, devices and systems for emulsion/droplet pcr


The present invention relates generally to the use of a class of surfactants for emulsion and droplet polymerase chain reaction (“pcr”) mixtures. The class of surfactants consists of those having the chemical formula r—(och2ch2)n—oh, wherein r is an alkyl group consisting of 12 to 18 carbons and n is 2 to 25.
Canon U.s. Life Sciences, Inc.


07/02/15
20150184145 

Novel compounds for use in pcr systems and applications thereof


This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (pcr). Methods for preparing the novel compounds are also described..
Life Technologies Corporation


07/02/15
20150182969 

Clamp for fast pcr heating


This disclosure provides a thermocycler system for performing polymerase chain reaction. The thermocycler system can comprise a plurality of bus bars, a microplate, a clamp and a current application device in electrical communication with the bus bars.
Bjs Ip Limited


06/25/15
20150176063 

Removal of pcr inhibitors


Provided herein is technology relating to processing and preparing samples and particularly, but not exclusively, to methods, systems, and kits for removing assay inhibitors, e.g., compounds that inhibit polymerase chain reaction, from samples comprising nucleic acids. In particular, the technology is directed toward treating crude sample preparations, such as supernatants from homogenized stool samples, with insoluble polyvinylpyrrolidone (pvp) to form pvp-assay inhibitor complexes, and filtration to separate the pvp-assay inhibitor complexes from the crude sample preparations to produce clarified samples that exhibit reduced assay inhibition..
Exact Sciences Corporation


06/25/15
20150176061 

Method for screening reagents used in pcr assays


The present invention relates to methods for screening of reagents used in the performance of polymerase chain reaction (pcr) assays. The invention has applications for genotyping, pathogen detection and in vitro diagnostics..
Roche Molecular Systems, Inc.


06/18/15
20150168434 

System for pcr sample preparation and for pcr set-up


A method of setting up a polymerase chain reaction (“pcr”) includes providing a loading device configured to receive sample tubes for setting up a pcr; providing a tube holding device comprising at least a first receiving opening; attaching the tube holding device to the loading device; and aligning at least one receiving opening of the tube holding device with one of the first and second receiving openings of the loading device and simultaneously covering the other of the first and the second receiving openings of the loading device.. .
Vela Operations Pte. Ltd.


06/18/15
20150168388 

Multiplexed analyses of test samples


The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule.
Somalogic, Inc.


06/18/15
20150167067 

Methods and compositions for universal detection of nucleic acids


Provided are methods and compositions for detecting the presence or amount of one or more target nucleic acids in a sample. Methods of the present invention include linking universal nucleic acid segments into a single molecule in a linking reaction dependent on a target nucleic acid of interest.
Unitaq Bio


06/11/15
20150159224 

Ultrasensitive detection and characterization of clustered kras mutations using peptide nucleic acid clamp pcr in drop-based microfluidics


This disclosure employs the combination of a microfluidics platform and drop-based digital polymerase chain reaction (dpcr) to create a breakthrough technology that enables the detection of ctc genes and the isolation of single ctcs from the blood. In the first method, cdna molecules from lysed ctcs are amplified in microfluidic drops and detected via fluorescence signal.

05/07/15
20150126382 

Technique combining pcr and loop-mediated isothermal amplification for the detection of nucleic acids


The present invention relates to a method and a kit of parts for detecting the presence or absence of one or more target nucleic acid sequences in a sample, the method comprising a sequence of steps for pre-amplifying the sample by means of a polymerase chain reaction, followed by a sequence of steps comprising an isothermal amplification of the pre-amplified sample, wherein the isothermal amplification comprises a pair of primers comprising a forward primer having a 3′ part that is substantially complementary to a first part of the target sequence, the presence or absence of which is to be detected, and a 5′ part that is substantially homolog to a second part of the target sequence, and a reverse primer comprising a 3′ part that is substantially homolog to a fourth part of the target sequence and a 5′ part that is substantially complementary to a third part of the target sequence.. .

05/07/15
20150125852 

Method for evaluation of viability of viruses with lymphotropism properties


Methods and techniques to increase the reliability of detecting virus infections, particularly lymphotropism, to eliminate false negative reactions in testing blood for the presence of lymphotropic viruses during enzyme immunoassay (eia) and polymerase chain reaction (pcr) testing, and to better detect viruses with lymphotropism in biological materials having a concentration of virus particles lower than the sensitivity threshold of existing eia and pcr methods, thereby making the techniques of the present invention more reliable.. .
Obschestvo S Ogranichennoi Otvetstvennostyu


04/16/15
20150104848 

Nucleic acid-free thermostable enzymes and methods of production thereof


The present invention provides thermostable enzymes, such as dna polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (pcr)..
Life Technologies Corporation


04/16/15
20150104798 

Microdroplet-manipulation automated execution of molecular biological protocols


Disclosed herein are automated systems for performing various biochemical and molecular biological procedures, including processor-controlled execution of protocols involving multiple steps performed in, on, or with liquid microdroplets. Example protocols are the various polymerase chain reaction (pcr) protocols, but the subject systems are not limited to performing pcr protocols.
The Arizona Board Of Regents On Behalf Of The University Of Arizona


04/09/15
20150099659 

Multiplex probes


Methods and reagents suitable for conducing polymerase chain reaction are described. In particular, the disclosure provides probes and primers that are suitable in dynamic flux amplification procedures.
Fluoresentric, Inc


03/26/15
20150087546 

Sequence, technique platform, and in vitro detecting clostridium difficile ribotype 027


The invention relates to a sequence, a technique platform, and a method for in vitro detecting clostridium difficile ribotype 027. The technology platform includes a pair of primers specific to c.
National Cheng Kung University


03/19/15
20150080259 

Systems and methods for performing amplicon rescue multiplex polymerase chain reaction (pcr)


Embodiments of the present disclosure generally pertain to systems and methods for performing amplicon rescue multiplex polymerase chain reaction (arm-pcr). In one embodiment, the system comprises a processor and a reader coupled to a control element.

03/05/15
20150064775 

Random access polymerase chain reaction testing


A random access, high-throughput system and method for preparing a biological sample for polymerase chain reaction (pcr) testing are disclosed. The system includes a nucleic acid isolation/purification apparatus and a pcr apparatus.
Siemens Healthcare Diagnostics Inc.


02/26/15
20150056171 

Methods of predicting clinical outcome of chronic lymphocytic leukemia


Provided are methods of predicting the outcome of a chronic lymphocytic leukemia (cll) in a subject. The methods comprise performing a polymerase chain reaction assay for an igh locus on a nucleic acid from a biological sample from a subject with cll, wherein the pcr assay amplifies a non-coding region of the igh locus, sequencing a product from the pcr assay, and determining a level of mutation in the non-coding region of the igh locus.
University Of Rochester


02/19/15
20150050660 

Stable compositions for nucleic acid amplification and sequencing


The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable dna polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing.
Life Technologies Corporation


02/12/15
20150044727 

Adaptive thermal block temperature control method and system


Aspects of the present teachings describe a method and apparatus for automatically controlling a block temperature to reduce undershooting and overshooting of the temperatures of a sample contained in the block and participating in a polymerase chain reaction (pcr). The adaptive thermal block temperature control begins when a sample temperature enters a sample window region between a preliminary setpoint temperature and a target setpoint temperature for the sample.
Applied Biosystems, Llc


02/12/15
20150044683 

Composition for hot-start reverse transcription reaction or hot-start reverse transcription polymerase chain reaction


A composition for hot-start reverse transcription reaction and a composition for reverse transcription pcr are disclosed. The composition is obtained by adding pyrophosphate and pyrophosphatase to an aqueous solution containing reaction buffer solution, mgcl2, four kinds of dntps, and reverse transcription polymerase in a single reaction tube.
Bioneer Corporation


02/05/15
20150038341 

Digital amplification


The identification of pre-defined mutations expected to be present in a minor fraction of a cell population is important for a variety of basic research and clinical applications. The exponential, analog nature of the polymerase chain reaction is transformed into a linear, digital signal suitable for this purpose.
The Johns Hopkins University


02/05/15
20150038336 

Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions


The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction.
Cornell University


02/05/15
20150037795 

Method and primer set for detecting mutation


Disclosed herein is a polymerase chain reaction (pcr)-based method for detecting an insertion/deletion variant, as compared with a reference sequence, in a selected region of a target gene in a sample. The target gene has a template strand and a coding strand complementary to the template strand.
Mackay Memorial Hospital


01/29/15
20150031029 

Method of detecting residual genomic dna and a kit thereof


The present disclosure relates to a highly specific and sensitive method of detecting host cell impurities in a biological sample by using quantitative real time polymerase chain reaction (q pcr). The present disclosure also provides novel designed primer and probe to amplify only the specific alu family of dispersed repetitive sequences from chinese hamster ovary cells used for expression of therapeutic proteins..
Biocon Limited


01/22/15
20150024434 

Method for obtaining fab fragments from single antibody producing cells by multiplexed cpr in combination with taqman probes


Herein is reported a method for a multiplex one tube real-time reverse-transcriptase gene-specific polymerase chain reaction for the amplification and quantification of cognate igg heavy and light chains encoding nucleic acids (human igg isotype) from a single cell.. .
Hoffmann-la Roche Inc.


01/22/15
20150020532 

Active, micro-well thermal control subsystem


Devices and systems for active thermal control of sample holding devices for bdna testing, polymerase chain reaction testing, chemiluminescent immuno-assay testing, and so forth. The thermal control subsystem includes a fluidic circuit, first and second heater assemblies, a centrifugal pump, and a heat exchange device.
Siemens Healthcare Diagnostics Inc.


01/15/15
20150018249 

Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions


The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (pcr) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence.
Cornell Research Foundation, Inc.


01/15/15
20150017688 

Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)


The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (rt-pcr). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step rt-pcr procedure using combinations of reverse transcriptase and thermostable dna polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin.
Life Technologies Corporation


01/15/15
20150017654 

Absolute pcr quantification


The present application provides methods and devices for absolute quantification of polymerase chain reaction target nucleic acids. In particular, the methods and devices of the present application provide for splitting a nucleic acid sample to be analyzed into small, isolated volumes, conducting the method of polymerase chain reaction (pcr) on said volumes, detecting pcr amplification products, analyzing said detected pcr amplification products, performing absolute quantification of the pcr target and presenting said quantification results..
The Research Foundation For The State University Of New York


01/15/15
20150017653 

Absolute pcr quantification


The present application provides methods and devices for absolute quantification of polymerase chain reaction target nucleic acids. In particular, the methods and devices of the present application provide for splitting a nucleic acid sample to be analyzed into small, isolated volumes, conducting the method of polymerase chain reaction (pcr) on said volumes, detecting pcr amplification products, analyzing said detected pcr amplification products, performing absolute quantification of the pcr target and presenting said quantification results..
The Research Foundation For The State University Of New York


01/15/15
20150017645 

Qualitative and quantitative detection of microbial nucleic acids


The present invention relates to new methods and uses for the qualitative and quantitative detection of microbial nucleic acids using at least a first control nucleic acid, or a first and a second control nucleic acid in different concentrations. The method is based on amplification of nucleic acids, for example the polymerase chain reaction.
Roche Molecular Systems, Inc.


12/25/14
20140377810 

Ssb-polymerase fusion proteins


Fusion proteins comprising a single strand dna binding protein and a nucleic acid polymerase (e.g. Dna polymerase or reverse transcriptase).
Life Technologies Corporation


12/25/14
20140377757 

Compositions and methods for screening for creatine transporter deficiency


Amplification primers, sequencing primers, kits for screening, and screening methods for identifying a slc6a8 creatine transporter gene mutation are disclosed. The screening method includes treating a sample of dna with polymerase chain reaction amplification primers for amplifying regions of the dna having slc6a8 to produce a first, second, and third amplification product, sequencing the first, second, and third amplification products with sequencing primer pairs to provide a dna sequence of slc6a8 in the sample, and comparing the dna sequence of slc6a8 with a reference dna sequence of slc6a8..
University Of Cincinnati


12/11/14
20140360879 

Control of chemical reactions using isotachophoresis


Isotachophoresis (itp) is exploited to control various aspects of chemical reactions. In a first aspect, at least one of the reactants of a chemical reaction is confined to an itp zone, but the resulting product of the chemical reaction is separated from this itp zone by the itp process.

11/27/14
20140349293 

Method and kit for protozoa characterization


Disclosed are compositions, kits, and methods for detecting, extracting, visualizing, characterizing, and/or identifying one or more protozoa. Various types of polymerase chain reaction techniques in connection with specifically designed oligonucleotide probes can be used to detect a variety of, e.g., pathogenic, protozoa in samples..

11/20/14
20140342366 

Modification of sample preparation to differentiate live and dead bacteria by polymerase chain reaction assay


The invention relates to a method of determining whether a live microbe, such as bacteria, is present in a test sample.. .

11/13/14
20140335525 

Cutoff point delta ct. her2 pcr testing in breast cancer


The present invention is related to an improved method for her2 gene test by using quantitative real-time pcr (polymerase chain reaction) technique. Our invention streamlines test process, and incorporates quality control for each major step, including sample, reagent, operation, and data report.

11/06/14
20140328733 

Simplified gating sealing and flow control in micro and nano devices


A biochip for multiplex genetic identification is disclosed. An biochip for separating and detecting a plurality of dna fragments includes a set of inputs and chambers for receiving a sample matrix of genetic material and reagents needed to conduct a polymerase chain reaction amplification of the genetic material.

10/23/14
20140315757 

Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions


The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (pcr) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence.

10/23/14
20140315202 

Method for assembling multiple target loci to single nucleic acid sequence


A method for assembling multiple target loci to a single assembled nucleic acid sequence involves assembling the multiple target loci to a single assembled nucleic acid sequence through two polymerase chain reactions (pcrs). A pair of primers for a primary amplification include a target-specific sequence and a 5′-flanking assembly spacer sequence.

10/23/14
20140315201 

Method for high-throughput screening of transgenic plants


The invention relates to a method for quantifying levels of expression and/or quantifying copy number of a heterologous polynucleotide in a transgenic plant using quantitative or real-time polymerase chain reaction (qpcr or real-time pcr), wherein the real-time pcr is performed using a primer set specific to a heterologous terminator sequence operably linked to the heterologous polynucleotide.. .



Polymerase Chain Reaction topics: Polymerase Chain Reaction, Polymerase, Nucleic Acid, Nucleotide, Quantitative, Amplification, Chlamydia Trachomatis, Nucleic Acids, Transcription, Oligonucleotide, Sequencing, Real Time Pcr, Differentiation, Lymphogranuloma Venereum, Recombinant

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