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Polymerase Chain Reaction patents



      
           
This page is updated frequently with new Polymerase Chain Reaction-related patent applications. Subscribe to the Polymerase Chain Reaction RSS feed to automatically get the update: related Polymerase RSS feeds. RSS updates for this page: Polymerase Chain Reaction RSS RSS


System for pcr sample preparation and for pcr set-up

Vela Operations Pte.

System for pcr sample preparation and for pcr set-up

System for pcr sample preparation and for pcr set-up

Somalogic

Multiplexed analyses of test samples

Date/App# patent app List of recent Polymerase Chain Reaction-related patents
06/25/15
20150176063
 Removal of pcr inhibitors patent thumbnailRemoval of pcr inhibitors
Provided herein is technology relating to processing and preparing samples and particularly, but not exclusively, to methods, systems, and kits for removing assay inhibitors, e.g., compounds that inhibit polymerase chain reaction, from samples comprising nucleic acids. In particular, the technology is directed toward treating crude sample preparations, such as supernatants from homogenized stool samples, with insoluble polyvinylpyrrolidone (pvp) to form pvp-assay inhibitor complexes, and filtration to separate the pvp-assay inhibitor complexes from the crude sample preparations to produce clarified samples that exhibit reduced assay inhibition..
Exact Sciences Corporation
06/25/15
20150176061
 Method for screening reagents used in pcr assays patent thumbnailMethod for screening reagents used in pcr assays
The present invention relates to methods for screening of reagents used in the performance of polymerase chain reaction (pcr) assays. The invention has applications for genotyping, pathogen detection and in vitro diagnostics..
Roche Molecular Systems, Inc.
06/18/15
20150168434
 System for pcr sample preparation and for pcr set-up patent thumbnailSystem for pcr sample preparation and for pcr set-up
A method of setting up a polymerase chain reaction (“pcr”) includes providing a loading device configured to receive sample tubes for setting up a pcr; providing a tube holding device comprising at least a first receiving opening; attaching the tube holding device to the loading device; and aligning at least one receiving opening of the tube holding device with one of the first and second receiving openings of the loading device and simultaneously covering the other of the first and the second receiving openings of the loading device.. .
Vela Operations Pte. Ltd.
06/18/15
20150168388
 Multiplexed analyses of test samples patent thumbnailMultiplexed analyses of test samples
The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule.
Somalogic, Inc.
06/18/15
20150167067
 Methods and compositions for universal detection of nucleic acids patent thumbnailMethods and compositions for universal detection of nucleic acids
Provided are methods and compositions for detecting the presence or amount of one or more target nucleic acids in a sample. Methods of the present invention include linking universal nucleic acid segments into a single molecule in a linking reaction dependent on a target nucleic acid of interest.
Unitaq Bio
06/11/15
20150159224
 Ultrasensitive detection and characterization of clustered kras mutations using peptide nucleic acid clamp pcr in drop-based microfluidics patent thumbnailUltrasensitive detection and characterization of clustered kras mutations using peptide nucleic acid clamp pcr in drop-based microfluidics
This disclosure employs the combination of a microfluidics platform and drop-based digital polymerase chain reaction (dpcr) to create a breakthrough technology that enables the detection of ctc genes and the isolation of single ctcs from the blood. In the first method, cdna molecules from lysed ctcs are amplified in microfluidic drops and detected via fluorescence signal.
05/07/15
20150126382
 Technique combining pcr and loop-mediated isothermal amplification for the detection of nucleic acids patent thumbnailTechnique combining pcr and loop-mediated isothermal amplification for the detection of nucleic acids
The present invention relates to a method and a kit of parts for detecting the presence or absence of one or more target nucleic acid sequences in a sample, the method comprising a sequence of steps for pre-amplifying the sample by means of a polymerase chain reaction, followed by a sequence of steps comprising an isothermal amplification of the pre-amplified sample, wherein the isothermal amplification comprises a pair of primers comprising a forward primer having a 3′ part that is substantially complementary to a first part of the target sequence, the presence or absence of which is to be detected, and a 5′ part that is substantially homolog to a second part of the target sequence, and a reverse primer comprising a 3′ part that is substantially homolog to a fourth part of the target sequence and a 5′ part that is substantially complementary to a third part of the target sequence.. .
05/07/15
20150125852
 Method for evaluation of viability of viruses with lymphotropism properties patent thumbnailMethod for evaluation of viability of viruses with lymphotropism properties
Methods and techniques to increase the reliability of detecting virus infections, particularly lymphotropism, to eliminate false negative reactions in testing blood for the presence of lymphotropic viruses during enzyme immunoassay (eia) and polymerase chain reaction (pcr) testing, and to better detect viruses with lymphotropism in biological materials having a concentration of virus particles lower than the sensitivity threshold of existing eia and pcr methods, thereby making the techniques of the present invention more reliable.. .
Obschestvo S Ogranichennoi Otvetstvennostyu
04/16/15
20150104848
 Nucleic acid-free thermostable enzymes and methods of production thereof patent thumbnailNucleic acid-free thermostable enzymes and methods of production thereof
The present invention provides thermostable enzymes, such as dna polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (pcr)..
Life Technologies Corporation
04/16/15
20150104798
 Microdroplet-manipulation  automated execution of molecular biological protocols patent thumbnailMicrodroplet-manipulation automated execution of molecular biological protocols
Disclosed herein are automated systems for performing various biochemical and molecular biological procedures, including processor-controlled execution of protocols involving multiple steps performed in, on, or with liquid microdroplets. Example protocols are the various polymerase chain reaction (pcr) protocols, but the subject systems are not limited to performing pcr protocols.
The Arizona Board Of Regents On Behalf Of The University Of Arizona
04/09/15
20150099659

Multiplex probes


Methods and reagents suitable for conducing polymerase chain reaction are described. In particular, the disclosure provides probes and primers that are suitable in dynamic flux amplification procedures.
Fluoresentric, Inc
03/26/15
20150087546

Sequence, technique platform, and in vitro detecting clostridium difficile ribotype 027


The invention relates to a sequence, a technique platform, and a method for in vitro detecting clostridium difficile ribotype 027. The technology platform includes a pair of primers specific to c.
National Cheng Kung University
03/19/15
20150080259

Systems and methods for performing amplicon rescue multiplex polymerase chain reaction (pcr)


Embodiments of the present disclosure generally pertain to systems and methods for performing amplicon rescue multiplex polymerase chain reaction (arm-pcr). In one embodiment, the system comprises a processor and a reader coupled to a control element.
03/05/15
20150064775

Random access polymerase chain reaction testing


A random access, high-throughput system and method for preparing a biological sample for polymerase chain reaction (pcr) testing are disclosed. The system includes a nucleic acid isolation/purification apparatus and a pcr apparatus.
Siemens Healthcare Diagnostics Inc.
02/26/15
20150056171

Methods of predicting clinical outcome of chronic lymphocytic leukemia


Provided are methods of predicting the outcome of a chronic lymphocytic leukemia (cll) in a subject. The methods comprise performing a polymerase chain reaction assay for an igh locus on a nucleic acid from a biological sample from a subject with cll, wherein the pcr assay amplifies a non-coding region of the igh locus, sequencing a product from the pcr assay, and determining a level of mutation in the non-coding region of the igh locus.
University Of Rochester
02/19/15
20150050660

Stable compositions for nucleic acid amplification and sequencing


The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable dna polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing.
Life Technologies Corporation
02/12/15
20150044727

Adaptive thermal block temperature control method and system


Aspects of the present teachings describe a method and apparatus for automatically controlling a block temperature to reduce undershooting and overshooting of the temperatures of a sample contained in the block and participating in a polymerase chain reaction (pcr). The adaptive thermal block temperature control begins when a sample temperature enters a sample window region between a preliminary setpoint temperature and a target setpoint temperature for the sample.
Applied Biosystems, Llc
02/12/15
20150044683

Composition for hot-start reverse transcription reaction or hot-start reverse transcription polymerase chain reaction


A composition for hot-start reverse transcription reaction and a composition for reverse transcription pcr are disclosed. The composition is obtained by adding pyrophosphate and pyrophosphatase to an aqueous solution containing reaction buffer solution, mgcl2, four kinds of dntps, and reverse transcription polymerase in a single reaction tube.
Bioneer Corporation
02/05/15
20150038341

Digital amplification


The identification of pre-defined mutations expected to be present in a minor fraction of a cell population is important for a variety of basic research and clinical applications. The exponential, analog nature of the polymerase chain reaction is transformed into a linear, digital signal suitable for this purpose.
The Johns Hopkins University
02/05/15
20150038336

Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions


The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction.
Cornell University
02/05/15
20150037795

Method and primer set for detecting mutation


Disclosed herein is a polymerase chain reaction (pcr)-based method for detecting an insertion/deletion variant, as compared with a reference sequence, in a selected region of a target gene in a sample. The target gene has a template strand and a coding strand complementary to the template strand.
Mackay Memorial Hospital
01/29/15
20150031029

Method of detecting residual genomic dna and a kit thereof


The present disclosure relates to a highly specific and sensitive method of detecting host cell impurities in a biological sample by using quantitative real time polymerase chain reaction (q pcr). The present disclosure also provides novel designed primer and probe to amplify only the specific alu family of dispersed repetitive sequences from chinese hamster ovary cells used for expression of therapeutic proteins..
Biocon Limited
01/22/15
20150024434

Method for obtaining fab fragments from single antibody producing cells by multiplexed cpr in combination with taqman probes


Herein is reported a method for a multiplex one tube real-time reverse-transcriptase gene-specific polymerase chain reaction for the amplification and quantification of cognate igg heavy and light chains encoding nucleic acids (human igg isotype) from a single cell.. .
Hoffmann-la Roche Inc.
01/22/15
20150020532

Active, micro-well thermal control subsystem


Devices and systems for active thermal control of sample holding devices for bdna testing, polymerase chain reaction testing, chemiluminescent immuno-assay testing, and so forth. The thermal control subsystem includes a fluidic circuit, first and second heater assemblies, a centrifugal pump, and a heat exchange device.
Siemens Healthcare Diagnostics Inc.
01/15/15
20150018249

Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions


The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (pcr) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence.
Cornell Research Foundation, Inc.
01/15/15
20150017688

Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)


The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (rt-pcr). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step rt-pcr procedure using combinations of reverse transcriptase and thermostable dna polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin.
Life Technologies Corporation
01/15/15
20150017654

Absolute pcr quantification


The present application provides methods and devices for absolute quantification of polymerase chain reaction target nucleic acids. In particular, the methods and devices of the present application provide for splitting a nucleic acid sample to be analyzed into small, isolated volumes, conducting the method of polymerase chain reaction (pcr) on said volumes, detecting pcr amplification products, analyzing said detected pcr amplification products, performing absolute quantification of the pcr target and presenting said quantification results..
The Research Foundation For The State University Of New York
01/15/15
20150017653

Absolute pcr quantification


The present application provides methods and devices for absolute quantification of polymerase chain reaction target nucleic acids. In particular, the methods and devices of the present application provide for splitting a nucleic acid sample to be analyzed into small, isolated volumes, conducting the method of polymerase chain reaction (pcr) on said volumes, detecting pcr amplification products, analyzing said detected pcr amplification products, performing absolute quantification of the pcr target and presenting said quantification results..
The Research Foundation For The State University Of New York
01/15/15
20150017645

Qualitative and quantitative detection of microbial nucleic acids


The present invention relates to new methods and uses for the qualitative and quantitative detection of microbial nucleic acids using at least a first control nucleic acid, or a first and a second control nucleic acid in different concentrations. The method is based on amplification of nucleic acids, for example the polymerase chain reaction.
Roche Molecular Systems, Inc.
12/25/14
20140377810

Ssb-polymerase fusion proteins


Fusion proteins comprising a single strand dna binding protein and a nucleic acid polymerase (e.g. Dna polymerase or reverse transcriptase).
Life Technologies Corporation
12/25/14
20140377757

Compositions and methods for screening for creatine transporter deficiency


Amplification primers, sequencing primers, kits for screening, and screening methods for identifying a slc6a8 creatine transporter gene mutation are disclosed. The screening method includes treating a sample of dna with polymerase chain reaction amplification primers for amplifying regions of the dna having slc6a8 to produce a first, second, and third amplification product, sequencing the first, second, and third amplification products with sequencing primer pairs to provide a dna sequence of slc6a8 in the sample, and comparing the dna sequence of slc6a8 with a reference dna sequence of slc6a8..
University Of Cincinnati
12/11/14
20140360879

Control of chemical reactions using isotachophoresis


Isotachophoresis (itp) is exploited to control various aspects of chemical reactions. In a first aspect, at least one of the reactants of a chemical reaction is confined to an itp zone, but the resulting product of the chemical reaction is separated from this itp zone by the itp process.
11/27/14
20140349293

Method and kit for protozoa characterization


Disclosed are compositions, kits, and methods for detecting, extracting, visualizing, characterizing, and/or identifying one or more protozoa. Various types of polymerase chain reaction techniques in connection with specifically designed oligonucleotide probes can be used to detect a variety of, e.g., pathogenic, protozoa in samples..
11/20/14
20140342366

Modification of sample preparation to differentiate live and dead bacteria by polymerase chain reaction assay


The invention relates to a method of determining whether a live microbe, such as bacteria, is present in a test sample.. .
11/13/14
20140335525

Cutoff point delta ct. her2 pcr testing in breast cancer


The present invention is related to an improved method for her2 gene test by using quantitative real-time pcr (polymerase chain reaction) technique. Our invention streamlines test process, and incorporates quality control for each major step, including sample, reagent, operation, and data report.
11/06/14
20140328733

Simplified gating sealing and flow control in micro and nano devices


A biochip for multiplex genetic identification is disclosed. An biochip for separating and detecting a plurality of dna fragments includes a set of inputs and chambers for receiving a sample matrix of genetic material and reagents needed to conduct a polymerase chain reaction amplification of the genetic material.
10/23/14
20140315757

Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions


The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (pcr) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence.
10/23/14
20140315202

Method for assembling multiple target loci to single nucleic acid sequence


A method for assembling multiple target loci to a single assembled nucleic acid sequence involves assembling the multiple target loci to a single assembled nucleic acid sequence through two polymerase chain reactions (pcrs). A pair of primers for a primary amplification include a target-specific sequence and a 5′-flanking assembly spacer sequence.
10/23/14
20140315201

Method for high-throughput screening of transgenic plants


The invention relates to a method for quantifying levels of expression and/or quantifying copy number of a heterologous polynucleotide in a transgenic plant using quantitative or real-time polymerase chain reaction (qpcr or real-time pcr), wherein the real-time pcr is performed using a primer set specific to a heterologous terminator sequence operably linked to the heterologous polynucleotide.. .
10/16/14
20140308708

Error correction in nucleic acid molecules


An enzymatic method for removing sequence errors in nucleic acid molecules are described. The method utilizes a cel endonuclease that cuts heteroduplexes at mismatch sites containing the errors and an overlap extension polymerase chain reaction to re-assemble the cleaved fragments into full-length nucleic acid molecules free of the errors..
10/16/14
20140308666

Development of novel detergents for use in pcr systems


This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (pcr). Methods for preparing the modified detergents are also described..
10/09/14
20140302562

Fast pcr heating


Provided herein is a microplate for polymerase chain reaction (pcr), comprising a substrate formed of a material that is susceptible to heating pcr samples upon the application of an electromagnetic field and/or electromagnetic energy to said substrate. The substrate provides a pcr ramp rate of at least 5° c./second upon the application of an electromagnetic field and/or electromagnetic energy to said substrate..
10/09/14
20140302503

Compositions, methods and systems for polymerase chain reaction assays


The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddpcr) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddpcr assays using intercalating dyes.
10/02/14
20140295441

Cartridge interface module


A cartridge interface module (cim), configured to engage with a removable microfluidic cartridge in a nucleic acid analyzer system can include a fluidics component, which is configured to initiate and support a liquid extraction of nucleic acids from a biological sample contained in the removable microfluidic cartridge. The cim also includes a polymerase chain reaction (pcr) assembly component which can be configured to initiate and support amplification of the extracted nucleic acids.
10/02/14
20140295434

Detection micro-rna with high specificity


The invention provides a method for detecting mirna based on polymerase chain reaction comprising evagreen dye, and the use of evagreen dye in elevating the binding specificity of primers to templates in pcr.. .
09/25/14
20140288289

Method for detecting and quantifying wheat endogenous gene


Provided is a method of detecting or quantifying a wheat species-specific dna in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having at partial sequence of a nucleotide sequence identified as seq id no: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule..
09/25/14
20140287420

Microfluidics polymerase chain reaction and high resolution melt detection


The present invention relates to a method and system for polymerase chain reaction (“pcr”), high resolution melt (“hrm”) analysis and microfluidics, and, more specifically, to a method and system for implementing the processes of pcr and hrm on a microscale in a microfluidics chamber for certain purposes including for purposes of dna detection and/or extraction.. .
09/25/14
20140287405

Method for detecting and quantifying wheat endogenous gene


Provided is a method of detecting or quantifying a wheat species-specific dna in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having a partial sequence of a nucleotide sequence identified as seq id no: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule..
09/18/14
20140273185

Tactical and portable pcr/hrm genome identification system


The present invention relates to a polymerase chain reaction and high resolution melt genetic identification system, and, more specifically, to a tactical and portable polymerase chain reaction and high resolution melt genetic analysis and identification system that is configured to determine and communicate analysis and identification results and a tiered confidence/alert level related to the analysis and identification results.. .
09/18/14
20140273099

Methods and devices for non-therman polymerase chain reaction


Methods and devices for non-thermal pcr amplification of nucleic acid sequences. An electrical potential is applied to cause non-thermal separation of strands of a double-stranded nucleic acid or double-stranded nucleic acid/primer extension product..
09/18/14
20140272998

Diagnostic microrna profiling in cutaneous t-cell lymphoma (ctcl)


The present invention relates to the field of cancer-diagnostics. In particular the invention relates to a microrna expression signature that allows discriminating skin samples of cutaneous t-cell lymphomas (ctcl) from non-malignant (inflammantory) skin samples by use of quantitative polymerase chain reaction performed on reverse transcribed mirna.
09/18/14
20140272984

Microfluidic flow monitoring


The present invention relates to systems and methods of monitoring velocity or flow in channels, especially in microfluidic channels. In some embodiments, the present invention relates to systems and methods of monitoring velocity or flow rate in systems and methods for performing a real-time polymerase chain reaction (pcr) in a continuous-flow microfluidic system..
09/18/14
20140272977

Molecular targets and methods for formulation screening and preservative efficacy testing


Methods and kits are disclosed for distinguishing viable from nonviable microbial cells. The methods and kits are useful in the screening of cell culture formulations and the testing of preservative efficacy.
09/18/14
20140272927

Methods, devices, and systems for processing multiple assays background


Methods, devices, and systems for performing polymerase chain reaction (pcr) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both pcr temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the pcr temperature cycling for real-time pcr and/or during temperature ramping in the melt data acquisition.
09/04/14
20140249142

Discovery of a somatic mutation in myd88 gene in lymphoplasmacytic lymphoma


Diagnostic assays for facilitating the diagnosis of lymphoplasmacytic lymphoma (lpl) are provided. The method comprises assessing a biological sample of the subject for the presence of a mutation at position 38182641 in chromosome 3p22.2, wherein presence of the mutation is indicative that the subject has lpl.
09/04/14
20140248711

Apparatus and electrical detection of oligonucleotides through pore blockades


Systems and methods for specific nucleic acid (na) sequence detection that do not rely on polymerase chain reaction (pcr) for target sequence amplification and do not require any special reagents other than a complementary sequence capture probe conjugated to spherical beads.. .
09/04/14
20140248622

Telomere length measurement in formalin-fixed, paraffin embedded (ffpe) samples by quantitative pcr


Methods of reliably quantifying telomere length in cells or tissues that have been formalin fixed and paraffin embedded (ffpe) samples by quantitative polymerase chain reaction protocol and kits for use with such various methods are provided. The methods of the present invention may be used to predetermine an individual's response to treatment with a telomerase inhibitor, a telomere damaging agent or a telomerase activator..
09/04/14
20140248613

Automatic detection kit for detecting hla alleles using real-time polymerase chain reaction


Provided is an automatic detection kit for automatically detecting hla alleles using a real-time polymerase chain reaction (pcr). The real-time pcr is performed on dna isolated from a sample using a primer which is able to specifically bind to hla alleles and a fluorescent probe which is able to detect amplification of the hla alleles in real time, and the hla allele typing is performed by analyzing a fluorescence value obtained from the real-time pcr using an hla automatic typing..
08/28/14
20140243242

Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence


The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a single reaction method to co-amplify multiple subsequences of a nucleic acid fragment sequence (i.e., for example, at least two read pairs from a single library insert sequence).
08/28/14
20140242594

Methods and compositions for preparation of nucleic acids


A method for isolating genomic dna (gdna) from a biological material. In some embodiments, the method includes (a) contacting a sample that contains gdna with a solution of hydroxide and a detergent under conditions and for a time sufficient to degrade a cell wall, a cell membrane, a nuclear membrane, a nucleoprotein, or combinations thereof and/or to denature the gdna; (b) mixing into the solution resulting from step (a) a solution characterized by high salt and sufficient buffering capacity to reduce the ph of the solution to less than 10, thereby producing a neutralized solution; (c) centrifuging the sample at a speed and length of time sufficient to clarify the preparation; and (d) removing insoluble material from the neutralized and clarified preparation, whereby a solution of gdna is produced.
08/21/14
20140236496

Methods and systems for visualizing and evaluating data


A computer-implemented method of generating a digital polymerase chain reaction (dpcr) result is provided. The method includes detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period.
08/21/14
20140235464

Detection and quantification of biomolecules using mass spectrometry


The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry.
08/21/14
20140234850

Dna fragment detection method, dna fragment detection kit and the use thereof


The disclosure claims a cleaved deoxyribonucleic acid (dna) detection method, a dna fragment detection kit and use thereof. Wherein, the method includes the steps of: designing primers according to a test site or a test region of the dna fragment; cyclizing the dna fragment to obtain acyclized dna; implementing polymerase chain reaction (pcr) amplification for the cyclized dna by using the primers; and detecting the pcr amplification product.
08/14/14
20140228256

Method and kit for constructing plasma dna sequencing library


The disclosure relates a method and a kit for constructing a plasma deoxyribonucleic acid (dna) sequencing library. The method provided by the disclosure includes: extracting a plasma dna; making the plasma dna ligate to a sequencing linker, and purifying a ligation product; performing polymerase chain reaction (pcr) amplification for the purified ligation product, purifying the pcr amplification product, and obtaining the plasma dna sequencing library, wherein, the method does not include the step of performing 5′-terminus phosphorylation for the plasma dna.
08/14/14
20140228223

High throughput paired-end sequencing of large-insert clone libraries


The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a paired end sequencing method that improves the yield of long-distance genomic read pairs by constructing long-insert clone libraries (i.e., for example, a fosill library or a foscn library) and converting the long-insert clone library using inverse polymerase chain reaction amplification or shearing and recircularization of shortened fragments into a library of co-ligated clone-insert ends.
07/31/14
20140213485

Methods for preparing cdna from low quantities of cells


Methods for preparing cdna libraries from single and low quantities of cells are disclosed. The methods are based on the principles of multi-strand displacement amplification or semi-random primed polymerase chain reaction.
07/31/14
20140212884

Composition and methods for rt-pcr comprising an anionic polymer


The present invention is in the fields of molecular biology. The present invention is directed to novel compositions, methods and kits useful for the generation of nucleic acids from an rna template and further nucleic acid replication.


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Polymerase Chain Reaction topics: Polymerase Chain Reaction, Polymerase, Nucleic Acid, Nucleotide, Quantitative, Amplification, Chlamydia Trachomatis, Nucleic Acids, Transcription, Oligonucleotide, Sequencing, Real Time Pcr, Differentiation, Lymphogranuloma Venereum, Recombinant

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