This page is updated frequently with new Polymerase Chain Reaction-related patent applications.
|Methods for temperature-mediated nested polymerase chain reaction|
Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a first temperature and cool the reaction mixture to a second temperature repeatedly for a first plurality of cycles.
|Polymerase chain reaction device and polymerase chain reaction method|
A pcr device, in which a vessel is filled with a liquid which has a lower specific gravity than a reaction mixture and is immiscible with the reaction mixture, and a control section drives and controls a first heating section and a second heating section so that the liquid in an upper part in the vessel is brought to a first temperature and the liquid in a lower part is brought to a second temperature which is lower than the first temperature, and also drives and controls an electric field generation section to generate an electric field between a lower electrode and an upper electrode so that the reaction mixture in a spherical shape in the liquid moves up and down repeatedly between the upper part and the lower part of the liquid by a coulomb force due to the electric field.. .
Seiko Epson Corporation
|Microchannel chip, pcr method, and heating/cooling control apparatus|
A method for performing a polymerase chain reaction (pcr) process on a treatment target liquid using a microchannel chip and a heating/cooling control apparatus is provided. The microchannel chip includes: a first substrate layer that has an introducing channel and a discharging channel; and a second substrate layer that is disposed on the first substrate layer and has a second substrate layer body and a metal film, the second substrate layer body having a microchannel connected to the two channels, the metal film structuring an upper surface of the microchannel.
Panasonic Intellectual Property Management Co., Ltd.
|Thermal control device and methods of use|
Thermal control devices adapted to provide improved control and efficiency in temperature cycling are provided herein. Such thermal control device can include a thermoelectric cooler controlled in coordination with another thermal manipulation device to control an opposing face of the thermoelectric cooler and/or a microenvironment.
|Apparatus and electrical detection of oligonucleotides through pore blockades|
Systems and methods for specific nucleic acid (na) sequence detection that do not rely on polymerase chain reaction (pcr) for target sequence amplification and do not require any special reagents other than a complementary sequence capture probe conjugated to spherical beads.. .
The Regents Of The University Of California
|Method for reducing primer-dimer amplification|
The present invention reduces primer-dimer amplification in a multiplex polymerase chain reaction (pcr). When a first forward primer (f1) and a second reverse primer (r2) have a complementary region at their 3′ends, primer dimers may form.
Pillar Biosciences Inc.
|Detection kit for identifying genotype in depression patients and using the same|
The present invention relates to a rs6311 test kit, which includes a probe, a primer, and a polymerase chain reaction solution, wherein said probe sequence is as follows: rs6311t-fam: ctgtgagtgtctggc (seq. Id.
|Method for evaluation of viability of viruses with lymphotropism properties|
Methods and techniques to increase the reliability of detecting virus infections, particularly lymphotropism, to eliminate false negative reactions in testing blood for the presence of lymphotropic viruses during enzyme immunoassay (eia) and polymerase chain reaction (pcr) testing, and to better detect viruses with lymphotropism in biological materials having a concentration of virus particles lower than the sensitivity threshold of existing eia and pcr methods, thereby making the techniques of the present invention more reliable.. .
|Camera imaging system for a fluid sample assay and using same|
Apparatuses and for causing a point-of-care polymerase chain reaction and analyzing the polymerase chain reaction at the point-of-care, particularly when unwanted bubbles are present during the polymerase chain reaction, are described herein. In a general embodiment, a device for analysing a polymerase chain reaction in a fluid sample includes a current source configured to cause the polymerase chain reaction by heating the fluid sample within a target zone, a camera imaging device configured to record a plurality of images of the fluid sample in the target zone while the current source causes the polymerase chain reaction, and a controller configured to (i) distinguish wanted objects in the plurality of images from an unwanted object in the plurality of images, and (ii) determine whether the fluid sample tests positive or negative for a bacteria or virus based on the wanted objects..
|Device for analyzing a fluid sample and use of test card with same|
Apparatuses and methods related to a point-of-care portable assay device and the use thereof with a test card are described herein. In a general embodiment, a device for monitoring a polymerase chain reaction in a fluid sample includes a vacuum source configured to pull the fluid sample through a microchannel, a current source configured to cause the polymerase chain reaction while the fluid sample is located within the microchannel, a light source configured to illuminate the polymerase chain reaction while the current source causes the polymerase chain reaction, a camera imaging device configured to record an image of the polymerase chain reaction while the light source illuminates the polymerase chain reaction, and a controller configured to analyze the image of the polymerase chain reaction and output a resulting analysis of the polymerase chain reaction..
Immunoglobulin variable region libraries
Antigen-specific immunoglobulin v-regions are identified from a library of nucleic acids amplified using polymerase chain reaction using leader sequence-specific forward primers. The use of leader sequence primers allows all v-region sequences to be amplified (including those with extensive 5′ end mutations) without loss of the original 5′ v gene segment sequence.
Dna methylation analysis
Disclosed herein are methods for determining whether one or more cpg dinucleotides are methylated using linear after the exponential polymerase chain reaction (“late-pcr”) or linear-expo-linear polymerase chain reaction (“lel-pcr”).. .
Detection of infectious agents from environmental air dust
Embodiments of the present disclosure are directed to systems and methods for collection and analysis of environmental air dust (ead) within an individually ventilated cage rack (ivr) environment for detecting pathogens. The method includes collection of an ead sample by a collection media, isolation of a plurality of nucleic acids (e.g., rna and/or dna) representative of one or more infectious agents from the ead sample, optional reverse transcription of rna to cdna if the isolated nucleic acids contain rna, amplification of the cdna and/or dna (e.g., by polymerase chain reaction (pcr)), and assay interpretation.
Quantitative reverse transcription polymerase chain reaction kit using tissue and blood for early diagnosis and screening test for therapeutic agent of breast cancer
There are provided a method for an early diagnosis and a screening test for a therapeutic agent of breast cancer by using tissue and blood, and a quantitative reverse transcription polymerase chain reaction kit for same. According to the present invention, it is possible to provide help in more effective treatment and diagnosis of breast cancer through expression rates of her2 expressed in blood and a cancer-related marker in the blood in addition to a tissue specimen..
University Industry Foundation, Yonsei University Wonju Campus
Assay for chlamydia trachomatis by amplification and detection of chlamydia trachomatis pmpa gene
A region of the chlamydia trachomatis pmpa gene has been identified which is useful for performing amplification assays to determine specifically whether c. Trachomatis is present in the sample being tested.
Becton, Dickinson And Company
Non-thermal cycling for polymerase chain reaction
Techniques, systems, and devices are disclosed for non-thermal cycling of polymerase chain reaction (pcr). In one aspect, a method for cycling pcr includes receiving an electrolytic fluid including ions, primers, polymerase enzymes, nucleotides, and a double-stranded nucleic acid in a fluid chamber having a first electrode and a second electrode, applying an electric field across the first and the second electrodes to generate a first ph level of the electrolytic fluid to denature the double-stranded nucleic acid to at least partial single strands, and applying a second electric field across the first and second electrodes to produce a second ph level of the electrolytic fluid, in which the second ph level enables binding of a polymerase enzyme and a primer with a corresponding segment of the single strands..
The Regents Of The University Of California
Single-step dna preparation for polymerase chain reaction using magnetic chitosan microparticles
The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a pcr reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a pcr compatible ph are mechanically agitated to provide for cell lysis and simultaneous dna capture, and then serve as a solid support for the nucleic acid template during the pcr reaction.
Canon U.s. Life Sciences, Inc.
Thermal cycler apparatus and related methods
An apparatus for thermal cycling can transfer heat uniformly and efficiently. The apparatus can be used in a method that reduces condensation on sample wells.
Biofire Defense, Llc
Microfluidic flow monitoring
The present invention relates to systems and methods of monitoring velocity or flow in channels, especially in microfluidic channels. In some embodiments, the present invention relates to systems and methods of monitoring velocity or flow rate in systems and methods for performing a real-time polymerase chain reaction (pcr) in a continuous-flow microfluidic system..
Canon U.s. Life Sciences, Inc.
Method for testing a mutant gene through real time polymerase chain reaction using inhibition of 5'-flap endonuclease activity
The present invention relates to a method for detecting single nucleotide polymorphism (snp) using a feature that the 5′-flap endonuclease (fen) activity of dna polymerase is inhibited when a probe complementarily binds to the end of a polymerase chain reaction (pcr) product. More specifically, the present invention relates to a novel method wherein it was verified that, when a probe used for a real-time pcr complementarily binds to the end site of a pcr product, the 5′-fen activity of thermostable dna polymerase to the probe is inhibited, and thus when such a feature is used to make a design such that an snp site to be detected is located at the 5′-end site of the probe, the 5′-flap formation is induced according to the allele, thereby allowing effective snp detection..
Method for detecting chromosomal rearrangements
An object of the invention is an in vitro method for detecting chromosomal rearrangements between at least two specific chromosomal regions in a biological sample of a human subject, which comprises the steps of: a) isolating deoxyribonucleic acid (dna) molecules comprising said specific chromosomal regions from said biological sample, wherein said dna molecules have an average length of x base pairs; b)amplifying the dna molecules of step a) by a multiplex polymerase chain reaction assay, said assay comprising at least two sets of primers, wherein each set of primers is capable of hybridizing with a specific reference chromosomal region, and each set of primer comprises a plurality of primers, said primers being capable of hybridizing to a nucleic acid strand of one of the said specific chromosomal regions at sites regularly spaced of less than x/2 base pairs; and hybridizing the product of the amplification of step b) with at least one set of nucleic probes.. .
Assistance Publique - Hopitaux De Paris
Fast pcr heating
Provided herein is a microplate for polymerase chain reaction (pcr), comprising a substrate formed of a material that is susceptible to heating pcr samples upon the application of an electromagnetic field and/or electromagnetic energy to said substrate. The substrate provides a pcr ramp rate of at least 5° c./second upon the application of an electromagnetic field and/or electromagnetic energy to said substrate..
Bjs Ip Ltd.
Channels with cross-sectional thermal gradients
Provided herein are systems, devices, and methods for generating thermal gradients in channels and uses thereof. In particular, provided herein are system, methods, and devices employing first and second thermal layers positioned around a channel in order to create a thermal gradient across a cross-section of the channel having, for example, a nucleic acid denaturation zone, a nucleic acid annealing zone, and a nucleic acid polymerization zone.
Abbott Molecular Inc.
Methods and systems for volume variation modeling in digital pcr
A method for performing digital polymerase chain reaction (dpcr) is provided. The method includes partitioning a biological sample volume including a plurality of target nucleic acids into a plurality of partitions, where at least one partition includes at least one target nucleic acid.
Life Technologies Corporation
Method of preparing a reaction mixture and related products
The invention relates to a method of preparing a reaction mixture for polymerase chain reaction (pcr) assay and a solution set for pcr. The method comprises providing a sample solution comprising a biological sample to be amplified in said pcr assay and first colorant providing the solution a first color, providing a reagent solution comprising at least one other substance required for performing said assay and second colorant providing the solution a second color different from the first color, and mixing the sample solution and the first reagent solution for providing a mixed solution to be subjected to the pcr process, the mixed solution having, due to said first and second colorants, a third color different from the first and second colors.
Thermo Fisher Scientific Baltics Uab
Methods to distinguish waldenstrom's macroglobulinemia from igm monoclonal gammopathy of undetermined significance
Diagnostic assays for discriminating waldenstrom's macroglobulinemia from igm monoclonal gammopathy of undetermined significance are provided. The method comprises obtaining a biological sample from a subject in need thereof, performing an allele-specific polymerase chain reaction assay to determine in the biological sample a level of a transcript comprising a mutation at position 38182641 in chromosome 3p22.2, and providing a report indicating whether delta ct value of the biological sample is less than a reference value, wherein the subject has waldenstrom's macroglobulinemia if the delta ct value is less than the reference value..
Dana-farber Cancer Institute, Inc.
Nucleic acid detection and quantification
The present invention relates to methods and uses for the detection or quantification of newly-synthesized double-stranded target nucleic acid molecules in a sample during quantitative real-time polymerase chain reaction (qpcr) amplification. According to the invention, an intercalating dye recognizing double-stranded dna molecules with higher affinity than single-stranded dna molecules and a fluorophore-labeled oligonucleotide-probe being sequence specific for a target nucleic acid molecule are simultaneously employed, thus enabling quantification a specific target and total amount of a mixed nucleic acid population, and enabling assessing the cause of suboptimal pcr performance..
Monitoring dna amplification
A method and kits are provided for nucleic acid quantification and discrimination using surface plasmon resonance (spr). The method provided is able to significantly enhance the detection limit and multiplex the discrimination assay using the melting properties of the target dna on top of standard pcr reaction.
Katholieke Universiteit Leuven
Methods for full-length amplification of double-stranded linear nucleic acids of unknown sequences
An adaptor for use in amplifying all linear, double-stranded nucleic acid molecules of unknown sequences in a sample is disclosed. The adaptor consists of: (1) the first oligonucleotide (p-oligo) with a phosphate at the 5′ end and without an additional thymine nucleotide at the 3′ end; and (2) the second oligonucleotide (t˜oligo) with an extra 3′-t and without a 5′-phosphate.
Stabilized reverse transcriptase fustion proteins
Stabilized reverse transcriptase fusion proteins including a thermostable reverse transcriptase connected to a stabilizer protein are described. Attaching the stabilizer protein to the thermostable reverse transcriptase stabilizes the fusion protein and can aid in its purification, provide increased solubility, allow for longer storage, or allow the fusion protein to be used under more rigorous conditions such as higher temperature.
Board Of Regents, The University Of Texas System
Selective detection mycobacterium tuberculosis and nontuberculous mycobacteria and kit using same
The present invention relates to a method for specifically detecting mycobacterium tuberculosis and nontuberculous mycobacteria by simultaneously amplifying and analyzing target genes using various primers and probes, and a kit using same. The method of the present invention is capable of selectively detecting mycobacterium tuberculosis and nontuberculous mycobacteria with very high efficiency through a multiplex real-time polymerase chain reaction (pcr) using probes and primers specific to target genes (particularly, is6110, 16s rrna and β-actin).
Pcr module, pcr system having the same, and inspecting using the same
A polymerase chain reaction (pcr) module is detachably combined with a reader system. The reader system includes a central processing unit (cpu) receiving a photo sensing signal to calculate gene amplification amount in real time and generating a temperature control signal based on a temperature signal and a temperature control information.
Optolane Technologies Inc.
Porous structure and manufacturing same
A porous structure according to the present invention has a polymerase chain reaction (pct) primer inside pores thereof, and hence, even an inner portion thereof can be used unlike general structures of which only surfaces are used for amplification and detection, thereby maximizing reactivity. In addition, the differentiating of the kinds of primers contained in respective structures leads to detection of several kinds of target nucleic acids at the same and real-time analysis thereof at the same time, and thus is useful for multiplex real-time pcr..
Korea Institute Of Science And Technology
Analysis unit for performing a polymerase chain reaction, operating such an analysis unit, and producing such an analysis unit
An analysis unit for performing a polymerase chain reaction includes a cover element, a bottom element, at least one fluid channel, at least one pressure channel, and a film. The bottom element has at least one bottom hollow that defines a fluid-accommodating surface and an arrangement of microcavities, and that faces toward the cover element.
Robert Bosch Gmbh
The present invention provides a thermocycler comprising: a rotatable platform having a plurality of reaction wells or being adapted to receive a plurality of reaction containers, wherein the rotatable platform and/or the reaction wells are formed, at least in part, of a material which is adapted to be inductively heated by exposure to electromagnetic energy. An electromagnetic energy source is provided and is configured to direct electromagnetic energy at the rotatable platform, wherein the electromagnetic energy source surrounds a sufficient amount of the rotatable platform in order to heat the entire platform substantially simultaneously.
Bio Molecular Systems Pty Ltd
Adaptive thermal block temperature control method and system
Aspects of the present teachings describe a method and apparatus for automatically controlling a block temperature to reduce undershooting and overshooting of the temperatures of a sample contained in the block and participating in a polymerase chain reaction (pcr). The adaptive thermal block temperature control begins when a sample temperature enters a sample window region between a preliminary setpoint temperature and a target setpoint temperature for the sample.
Applied Biosystems, Llc
Varietal counting of nucleic acids for obtaining genomic copy number information
A method for obtaining from genomic material genomic copy number information unaffected by amplification distortion, comprising obtaining segments of the genomic material, tagging the segments with substantially unique tags to generate tagged nucleic acid molecules, such that each tagged nucleic acid molecule comprises one segment of the genomic material and a tag, subjecting the tagged nucleic acid molecules to amplification by polymerase chain reaction (pcr), generating tag associated sequence reads by sequencing the product of the pcr reaction, assigning each tagged nucleic acid molecule to a location on a genome associated with the genomic material by mapping the subsequence of each tag associated sequence read corresponding to a segment of the genomic material to a location on the genome, and counting the number of tagged nucleic acid molecules having a different tag that have been assigned to the same location on the genome, thereby obtaining genomic copy number information unaffected by amplification distortion.. .
Cold Spring Harbor Laboratory
Analysis unit for carrying out a polymerase chain reaction, analysis device, operating such an analysis unit, and producing such an analysis unit
An analysis unit is configured to carry out a polymerase chain reaction. The analysis unit includes a lid element with at least one lid recess and a base element with at least one base recess.
Robert Bosch Gmbh
Method for steadying thermal convection flow field in solution during thermal convective polymerase chain reaction
A method for steadying a thermal convection flow field in a pcr reaction solution during a thermal convective polymerase chain reaction (pcr) includes steps as follows. A pcr tube is provided.
Genereach Biotechnology Corp.
Dry composition of reaction compounds with stabilized polymerase
The present invention provides methods to obtain dry compositions of reaction compounds that maintain the biological activity of the compounds upon re-solubilization after a certain storage time. Preferably, the dry composition comprises a polymerase, and the dry composition is usable for polymerase chain reaction (pcr) amplification after re-solubilization..
Roche Molecular Systems, Inc.
Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution
A multiplexed polymerase chain reaction (pcr) dna detection system and a method for dna detection within the pcr system are provided. The pcr dna detection system includes a color charge-coupled device (ccd) camera, fluorophore-quencher probes and an imaging chamber.
Agency For Science, Technology And Research
Method of de-differentiating and re-differentiating somatic cells using rna
Rna prepared by in vitro transcription using a polymerase chain reaction (pcr)-generated template can be introduced into a cell to modulate cell activity. This method is useful in de-differentiating somatic cells to pluripotent, multipotent, or unipotent cells; re-differentiating stem cells into differentiated cells; or reprogramming of somatic cells to modulate cell activities such as metabolism.
Methods and devices for non-thermal polymerase chain reaction
Methods and devices for non-thermal pcr amplification of nucleic acid sequences. An electrical potential is applied to cause non-thermal separation of strands of a double-stranded nucleic acid or double-stranded nucleic acid/primer extension product..
The Regents Of The University Of California
Porcine torque teno virus vaccines and diagnosis
The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine torque teno virus (pttv) genotypes or subtypes pttv1a-va, pttv1b-va, pttv2b-va, and pttv2c-va. The present invention also provides infectious dna clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same.
Virginia Tech Intellectual Properties, Inc.
Control of chemical reactions using isotachophoresis
Isotachophoresis (itp) is exploited to control various aspects of chemical reactions. In a first aspect, at least one of the reactants of a chemical reaction is confined to an itp zone, but the resulting product of the chemical reaction is separated from this itp zone by the itp process.
The Board Of Trustees Of The Leland Stanford Junior University
Diagnostic methods for infectious disease using endogenous gene expression
Disclosed are methods of diagnosis of a pathogen-associated disease. These methods comprise: providing a biological sample from a human subject; determining presence, absence and/or quantity of a bacterial pathogen, a viral pathogen, or a combination thereof, by a pathogen culture, a serum antibody detection test, a pathogen antigen detection test, a pathogen dna and/or rna detection test, or a combination thereof; determining in the sample, expression levels of at least one endogenous gene in which aberrant expression levels are associated with infection with a pathogen, by a microarray hybridization assay, rna-seq assay, polymerase chain reaction assay, a lamp assay, a ligase chain reaction assay, a southern, northern, or western blot assay, an elisa or a combination thereof.
Devices for real-time polymerase chain reaction
An improved device and system for facilitating polymerase chain reaction including a light source, detector, waveguide, and filters that occupy minimal space and facilitate detection of stationary samples, reduced sample read time, and simultaneous reading of multiple light wavelengths.. .
Step-up cold-pcr enrichment
Methods of using polymerase chain reactions to enrich a plurality of target sequences in a sample containing reference sequences and target sequences having high homology and amplifiable by the same primer pairs are provided herein. In particular the methods provide a robust means to improve the fold enrichment of the target sequences and minimize reaction-to-reaction, well-to-well and run-to-run variations in the enrichment methods, e.g., in multiplex reactions..
Variety identification-encoding system and encoding method using the same
Provided is a variety identification-encoding system, including: a chromosome-decoding module decoding a chromosome of a reference genome variety and a chromosome of a target variety; a variation region-detecting module detecting a variation region in the decoded chromosome through single nucleotide variation dense region analysis; an amplification result-acquiring module setting an indel marker in the detected variation region and amplifying the indel marker by a polymerase chain reaction (pcr) to acquire an amplification result; and an encoding module encoding the amplification result.. .
Republic Of Korea (mngmnt : Rural Devel. Admin.)
Devices and methods for molecular diagnostic testing
A hand-held molecular diagnostic test device includes a housing, an amplification (or pcr) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume.
Reactivity-dependent and interaction-dependent pcr
Methods, reagents, compositions, and kits for reactivity-dependent polymerase chain reaction (rd-pcr) and interaction-dependent polymerase chain reaction (id-pcr) are provided herein. Rd-pcr is a technique useful for determining whether a reactive moiety can form a covalent bond to a target reactive moiety, for example, in screening a library of candidate reactive moieties for reactivity with a target reactive moiety, and in identifying an enzyme substrate, for example, in protease substrate profiling.
Real time quantitative and qualitative analysis biosubstance
A method of quantitatively and qualitatively analyzing a biomaterial in real-time, the method comprising preparing a device for detecting a biomaterial, feeding a complex of first and second probes, a forward primer, a reverse primer, a sample comprising deoxynucleotide triphosphate, a polymerase having exonuclease activity, and a sample comprising target genes, and a reaction solution comprising a buffer into the reaction container, performing polymerase chain reaction comprising denaturation of the target genes in the sample, hybridization of the target genes, the complex, and the forward and reverse primers in the sample, and elongation of the primers through the polymerase having exonuclease activity, allowing for elongation of the second probe on the third probe by the polymerase after hybridizing the released second probe and the third probe fixed to the biochip, detecting a first fluorescence signal by the first phosphor and a second fluorescence signal by the second phosphor.. .
Apparatus for high throughput chemical reactions
Apparatus, systems, chips, and methods of performing a large number of simultaneous chemical reactions are provided herein. The chips of the invention comprise addressable units that can be addressed according to the temperature of the reaction to be run.