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Polymerase Chain Reaction

Polymerase Chain Reaction-related patent applications - as published by the U.S. Patent and Trademark Office (USPTO).


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Method and system for screening nanobody
Bgi Shenzhen Co., Limited
August 10, 2017 - N°20170226566

Provided are a method for screening for nanobodies and a corresponding system. The method uses polymerase chain reactions and cdna 5′ end rapid amplification technology to screen for and obtain nanobodies. The experiment cycle requires only approximately 21 days.
Thermal uniformity for thermal cycler instrumentation using dynamic control
Life Technologies Corporation
August 10, 2017 - N°20170225169

A method for performing polymerase chain reactions (pcr) for improving thermal non-uniformity is provided. The method includes measuring a first temperature, by a first sensor, of a first sample block sector of a sample block and measuring a second temperature, by a second sensor, of a second sample block sector of the sample block that is adjacent to the first ...
In vitro method for the detection and quantification of hiv-2
Universite Paris Diderot
August 03, 2017 - N°20170218467

The present invention relates to a method for detecting or quantifying human immunodeficiency virus-2 (hiv-2) nucleic acids in a biological sample, comprising: a) performing a real-time polymerase chain reaction (pcr) or a real-time reverse transcriptase polymerase chain reaction (rt-pcr) on nucleic acids of the biological sample with: (i) at least 4 primers respectively comprising or consisting of: —sequence seq id ...
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Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ...
Cornell University
July 27, 2017 - N°20170212103

The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and ...
Quantitative reverse transcription polymerase chain reaction kit for breast cancer drug screening test and early ...
University Industry Foundation, Yonsei University Wonju Campus
July 27, 2017 - N°20170211156

There are provided an information offering method for diagnosing breast cancer comprising: (a) separating total rna from a cell obtained from a tissue or blood of a suspected cancer patient; (b) synthesizing a cdna from the separated total rna; (c) performing a real-time pcr with the synthesized cdna by using primer pair mix and probe mix that can amplify human ...
Process and molecular biomarker
Centro De Hemoterapia E Hematologia Do Para - Hemopa
July 20, 2017 - N°20170204464

A process and molecular biomarker for platelets stored in blood banks provides for efficient verification of which platelet concentrate bags are in good condition for transfusion by measuring, by real-time polymerase chain reaction, expression levels of hsa-mir-127 and hsa-mir-320a micrornas as the first process and biomarker predictor of platelet's cell aging during storage and the consequent presence of storage ...
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Devices and methods for molecular diagnostic testing
Click Diagnostics, Inc.
July 20, 2017 - N°20170203297

A hand-held molecular diagnostic test device includes a housing, an amplification (or pcr) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (pcr) on the input sample. The detection module is configured ...
Dna amplification technology
Fluoresentric, Inc.
July 13, 2017 - N°20170198342

Methods and reagents suitable for conducing polymerase chain reaction are described. In particular, a nucleic acid amplification design strategy and thermal cycling profile to enable efficient amplification of multiple nucleic acid targets along with improved sensitivity is disclosed. The present disclosure also describes methods and devices for increasing the melting temperature (tm) of a primer.
Detection of dna methylation using combined nuclease ligation reactions
Cornell University
July 06, 2017 - N°20170191113

The present invention is directed to methods for identifying the presence of one or more methylated or unmethylated target nucleotide sequences in a sample that involve coupled methylation sensitive restriction enzyme digestion-ligation and/or extension processes. In some embodiments, the ligation and primary extension products formed in the reaction processes of the present invention are subsequently amplified using a polymerase ...
Clamp for fast pcr heating
Bjs Ip Ltd
July 06, 2017 - N°20170189911

This disclosure provides a thermocycler system for performing polymerase chain reaction. The thermocycler system can comprise a plurality of bus bars, a microplate, a clamp and a current application device in electrical communication with the bus bars. The clamp is suitable for forming a low resistance electrical connection between the microplate and an individual bus bar of the plurality of ...
Human exhaled aerosol droplet biomarker system and method
University Of Maryland, College Park
June 29, 2017 - N°20170184609

A system and method for detecting a biomarker in exhaled breath condensate nanodroplets comprises noninvasively collecting exhaled breath condensate nanodroplets of a subject, and analyzing said nanodroplets utilizing immuno-quantitative polymerase chain reaction to detect one or more target biomarkers.
Methods and compositions relating to fusions of alk for diagnosing and treating cancer
Insight Genetics, Inc.
June 29, 2017 - N°20170183743

Disclosed are methods and compositions for detecting the presence of a cancer in a subject and assessing the efficacy of treatments for the same. The disclosed method use reverse transcription polymerase chain reaction (rt-pcr) and multiplex polymerase chain reaction techniques as well as template exchange extension reaction (teer) to detect the presence of point mutations, truncations, or fusions of anaplastic ...
Method of double allele specific pcr for snp microarray
Phalanx Biotech Group, Inc.
June 29, 2017 - N°20170183729

The present invention provides a method of double allele specific polymerase chain reaction (pcr) for single nucleotide polymorphism (snp) microarray, the method provides the allele specific site as the 3′ teiiiiinal nucleotide of forward and reverse primers, it does not require a primer having specific nucleotides. The method of the present invention is easily to design the primer based on ...
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Real-time pcr in micro-channels
Canon U.s. Life Sciences, Inc.
June 29, 2017 - N°20170182497

The present invention relates to methods for amplifying nucleic acids in micro-channels. More specifically, the present invention relates to methods for performing a real-time polymerase chain reaction (pcr) in a continuous-flow microfluidic system and to methods for monitoring real-time pcr in such systems.
Cartridge-based thermocycler
Click Diagnostics, Inc.
June 22, 2017 - N°20170173588

Cartridge-based thermocyclers can include cartridges that are configured to move a fluid between distinct chambers. In some cases, the cartridge-based thermocyclers can be used for thermocycling a sample fluid comprising a deoxyribonucleic acid (dna) target to perform polymerase chain reaction (pcr). Individual chambers can be heated, cooled, and/or compressed to mix fluid within the chamber or to propel fluid ...
Point of care polymerase chain reaction device for disease detection
Advanced Theranostics Inc.
June 22, 2017 - N°20170173585

A point-of-care device for detecting a target nucleic acid is provided. The device comprises: an extraction chamber adapted to receive a biological sample, wherein said extraction chamber comprises means to extract and lyse the sample to release nucleic acid; a first amplification chamber in communication with the extraction chamber, wherein said amplification chamber comprises means to trigger nucleic acid amplification ...
Methods for detecting nucleic acid fragments
Bio-id Diagnostic Inc.
June 15, 2017 - N°20170166954

Provided are methods for detecting and analyzing polynucleotides in a biological sample or sample derived therefrom for example, using a synthetic template polynucleotide. In some aspects, a target polynucleotide in the sample hybridizes to the template polynucleotide and is extended by a polymerase, generating an extended target polynucleotide. In some examples, the extended target polynucleotide is amplified, for example, by ...
Restriction mediated quantitative polymerase chain reactions
Multiplicom N.v.
May 18, 2017 - N°20170137865

The present invention relates to the technical field of nucleic acid amplification using a polymerase chain reaction (pcr). Specifically, the present invention relates to polymerase chain reaction (pcr) primers and polymerase chain reaction (pcr) nucleic acid amplification mixture and the use thereof in (quantitative) polymerase chain reactions (pcr). Specifically, the present invention relates to polymerase chain reaction (pcr) primers suitable ...
Polymerization of nucleic acids using proteins having low isoelectric points
Life Technologies Corporation
April 27, 2017 - N°20170114391

This disclosure relates to the use of one or more proteins (e. G., globular proteins) having a low isoelectric point and/or a limited number (e. G., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (pcr).
Method for obtaining immunoglobulin encoding nucleic acid
Life Technologies Corporation
April 20, 2017 - N°20170107550

Whereby the distance of the binding locations of the primer employed in the second polymerase chain reaction is reduced compared to the first polymerase chain reaction.
Method for detecting guanine-abasic site in dna
Life Technologies Corporation
March 30, 2017 - N°20170088884

(3) step 3 of performing polymerase chain reaction on the modified double-stranded dna obtained by conducting step 1 and step 2, which serves as a template, to search for the presence or absence of an amplification product, the sequence of steps 1 and 2 being not limited to the order presented.
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