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Polymerase Chain Reaction

Polymerase Chain Reaction-related patent applications - as published by the U.S. Patent and Trademark Office (USPTO).


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Cutoff point delta ct method for genetic pcr testing in human cancer
Genetics Development Corporation
January 11, 2018 - N°20180010191

The present invention is related to an improved method for her2 gene test by using quantitative real-time pcr (polymerase chain reaction) technique. Our invention streamlines test process, and incorporates quality control for each major step, including sample, reagent, operation, and data report. We eliminate the need for reference genes which is hard to standardize in her2 pcr test. We develop ...
Highly sensitive method for detection of viral hiv dna remaining after antiretroviral therapy of aids ...
January 04, 2018 - N°20180003666

Methods for detecting polynucleotides, especially the dna replicated from samples obtained from subjects infected with pathogenic viruses such as human immunodeficiency virus, by detecting electromagnetic signals (“ems”) emitted by such polynucleotides, and methods for improving the sensitivity of the polymerase chain reaction (“pcr”).
Methods and compositions for identifying and quantifying microbial dna
Advatect Diagnostics, Llc
December 28, 2017 - N°20170369952

This invention relates to methods and compositions for identifying microbial dna in the tissues or body fluid samples of patients. More particularly, the invention relates to two-step polymerase chain reaction based methods for identifying microbial dna in the tissues or body fluid samples of patients, and compositions therefor. Microbial dna can also be quantified using the methods described herein.
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Methods for rt-pcr comprising an anionic polymer
Qiagen Gmbh
December 14, 2017 - N°20170356026

The present invention is directed to methods for the generation of nucleic acids from an rna template and further nucleic acid replication. Specifically, the invention is directed to the generation and amplification of nucleic acids by reverse transcriptase-polymerase chain reaction (rt-pcr).
Linear-expo-linear pcr (lel-pcr)
Bdrandeis University
November 23, 2017 - N°20170335383

Disclosed herein is a nucleic acid amplification process referred to as linear-expo-linear polymerase chain reaction (lel-pcr).
Methods and compositions for characterizing drug resistant bacteria from formalin-fixed paraffin-embedded biological samples
American Molecular Laboratories, Inc.
November 16, 2017 - N°20170327873

The invention provides methods and compositions generally useful to the use of polymerase chain reaction (pcr) amplification of trace dna sequences from formalin-fixed paraffin-embedded (ffpe) biopsy samples and specifically relevant to the identification of multi-drug resistant h. Pylori in such biopsy samples.
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Porous matrix comprising nucleic acid primer-carbon material composites and pcr using the same
Korea Institute Of Science And Technology
November 09, 2017 - N°20170321265

A porous matrix according to the present disclosure, wherein a nucleic acid primer-carbon material composite in which one or more nucleic acid primer of a forward primer and a reverse primer as a polymerase chain reaction (pcr) primer is bound to a carbon material is included in the pores of the matrix, provides improved amplification efficiency as compared to a ...
Methods and reagents for reverse-transcription polymerase chain reaction
Ge Healthcare Uk Limited
November 09, 2017 - N°20170321250

The present invention provides a method of amplifying an rna molecule in a biological sample by reverse transcription pcr (rt-pcr), wherein the rt-pcr is carried out in a solution comprising a polar aprotic solvent; a serum albumin, and a polyol.
Methods for culturing and analyzing cells
Stokes Bio Limited
November 09, 2017 - N°20170320025

A system for analyzing a biological sample may include at least one sample acquisition stage comprising a sample acquisition device for acquiring the biological sample from a sample source; a droplet generator device for forming a droplet wrapped in an immiscible carrier fluid, wherein the wrapped droplet comprises at least the biological sample and a reagent, the droplet generator configured ...
Rapid thermocycler system for rapid amplification of nucleic acids and related methods
Streck, Inc.
October 26, 2017 - N°20170304828

A thermocycling device and method of operating a thermocycler instrument the instrument including a sample holder, at least one thermal cycling element, and at least one first and second temperature sensors, for causing the sample holder containing the at least one sample to undergo polymerase chain reaction amplification by repeated cycling between at least a denaturation heating stage and an ...
Interfacial effects enable droplet actuation, inhibition relief, and early sensing of polymerase chain reaction
October 19, 2017 - N°20170298416

Disclosed are devices, systems and methods of use utilizing interfacial effects enabling droplet actuation, inhibition and early sensing of molecular reactions, such as of polymerase chain reaction.
Binding and catalytic molecules built from l-dna with added nucleotides
October 19, 2017 - N°20170298356

This invention provides for processes for binding to and/or chemically transforming a preselected target, where the process involves contacting said target to an oligonucleotide molecule that contains one or more “non-standard” nucleotides, which are nucleotide analogs that, when incorporated into oligonucleotides (dna or rna, collectively xna), present to a pattern of hydrogen bonds that is ...
Qualitative and quantitative detection of microbial nucleic acids
Roche Molecular Systems, Inc.
October 12, 2017 - N°20170292145

The present invention relates to new methods and uses for the qualitative and quantitative detection of microbial nucleic acids using at least a first control nucleic acid, or a first and a second control nucleic acid in different concentrations. The method is based on amplification of nucleic acids, for example the polymerase chain reaction. Further provided are kits comprising components ...
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Devices and methods for molecular diagnostic testing
Click Diagnostics, Inc.
September 14, 2017 - N°20170259263

A hand-held molecular diagnostic test device includes a housing, an amplification (or pcr) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (pcr) on the input sample. The detection module is configured ...
Polymerase chain reaction primers and probes for mycobacterium tuberculosis
Rutgers, The State University Of New Jersey
August 31, 2017 - N°20170247747

The present invention relates to novel primers and sloppy molecular beacon and molecular beacon probes for amplifying segments from different genes in mycobacterium tuberculosis for identifying the presence of m. Tb dna and/or resistance to anti-tuberculosis drugs.
Two-stage thermal convection apparatus and uses thereof
Ahram Biosystems, Inc.
August 24, 2017 - N°20170239662

Disclosed is a multi-stage thermal convection apparatus such as a two-stage thermal convection apparatus and uses thereof. In one embodiment, the two-stage thermal convection apparatus includes a temperature shaping element that assists a thermal convection mediated polymerase chain reaction (pcr). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many ...
Three-stage thermal convection apparatus and uses thereof
Ahram Biosystems, Inc.
August 24, 2017 - N°20170239654

Disclosed is a multi-stage thermal convection apparatus and uses thereof. In one embodiment, the invention features a three-stage thermal convection apparatus that includes a temperature shaping element for assisting a thermal convection mediated polymerase chain reaction (pcr). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In ...
Tritrichomonas foetus nucleic acid detection methods
The Texas A&m University System
August 17, 2017 - N°20170233830

The present invention provides quantitative polymerase chain reaction (pcr) methods and kits for diagnosing trichomoniasis.
Borrellia provocation procedure protocol
August 17, 2017 - N°20170233793

The testing for the lyme disease pathogen, (borrelia bergdorferi (bb) and all other lyme borrelia), is notoriously difficult. Bb is not by nature a blood loving bacteria, and prefers the climate of avascular tissues such as cartilage. The borrelia provocation procedure disclosed herein proposes to uniquely lure the lyme borrelia spirochetes into the blood through a simple procedure of sub-dermal ...
Immunoglobulin variable region libraries
Xbiotech, Inc.
August 17, 2017 - N°20170233457

Antigen-specific immunoglobulin v-regions are identified from a library of nucleic acids amplified using polymerase chain reaction using leader sequence-specific forward primers. The use of leader sequence primers allows all v-region sequences to be amplified (including those with extensive 5′ end mutations) without loss of the original 5′ v gene segment sequence. These libraries can be screened for antigen-specific v-regions using ...
Method and system for screening nanobody
Bgi Shenzhen Co., Limited
August 10, 2017 - N°20170226566

Provided are a method for screening for nanobodies and a corresponding system. The method uses polymerase chain reactions and cdna 5′ end rapid amplification technology to screen for and obtain nanobodies. The experiment cycle requires only approximately 21 days.
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