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Polymerase Chain Reaction patents



      
           
This page is updated frequently with new Polymerase Chain Reaction-related patent applications. Subscribe to the Polymerase Chain Reaction RSS feed to automatically get the update: related Polymerase RSS feeds. RSS updates for this page: Polymerase Chain Reaction RSS RSS


Date/App# patent app List of recent Polymerase Chain Reaction-related patents
10/23/14
20140315757
 Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions patent thumbnailDetection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (pcr) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence.
10/23/14
20140315202
 Method for assembling multiple target loci to single nucleic acid sequence patent thumbnailMethod for assembling multiple target loci to single nucleic acid sequence
A method for assembling multiple target loci to a single assembled nucleic acid sequence involves assembling the multiple target loci to a single assembled nucleic acid sequence through two polymerase chain reactions (pcrs). A pair of primers for a primary amplification include a target-specific sequence and a 5′-flanking assembly spacer sequence.
10/23/14
20140315201
 Method for high-throughput screening of transgenic plants patent thumbnailMethod for high-throughput screening of transgenic plants
The invention relates to a method for quantifying levels of expression and/or quantifying copy number of a heterologous polynucleotide in a transgenic plant using quantitative or real-time polymerase chain reaction (qpcr or real-time pcr), wherein the real-time pcr is performed using a primer set specific to a heterologous terminator sequence operably linked to the heterologous polynucleotide.. .
10/16/14
20140308708
 Error correction in nucleic acid molecules patent thumbnailError correction in nucleic acid molecules
An enzymatic method for removing sequence errors in nucleic acid molecules are described. The method utilizes a cel endonuclease that cuts heteroduplexes at mismatch sites containing the errors and an overlap extension polymerase chain reaction to re-assemble the cleaved fragments into full-length nucleic acid molecules free of the errors..
10/16/14
20140308666
 Development of novel detergents for use in pcr systems patent thumbnailDevelopment of novel detergents for use in pcr systems
This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (pcr). Methods for preparing the modified detergents are also described..
10/09/14
20140302562
 Fast pcr heating patent thumbnailFast pcr heating
Provided herein is a microplate for polymerase chain reaction (pcr), comprising a substrate formed of a material that is susceptible to heating pcr samples upon the application of an electromagnetic field and/or electromagnetic energy to said substrate. The substrate provides a pcr ramp rate of at least 5° c./second upon the application of an electromagnetic field and/or electromagnetic energy to said substrate..
10/09/14
20140302503
 Compositions, methods and systems for polymerase chain reaction assays patent thumbnailCompositions, methods and systems for polymerase chain reaction assays
The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddpcr) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddpcr assays using intercalating dyes.
10/02/14
20140295441
 Cartridge interface module patent thumbnailCartridge interface module
A cartridge interface module (cim), configured to engage with a removable microfluidic cartridge in a nucleic acid analyzer system can include a fluidics component, which is configured to initiate and support a liquid extraction of nucleic acids from a biological sample contained in the removable microfluidic cartridge. The cim also includes a polymerase chain reaction (pcr) assembly component which can be configured to initiate and support amplification of the extracted nucleic acids.
10/02/14
20140295434
 Detection method of micro-rna with high specificity patent thumbnailDetection method of micro-rna with high specificity
The invention provides a method for detecting mirna based on polymerase chain reaction comprising evagreen dye, and the use of evagreen dye in elevating the binding specificity of primers to templates in pcr.. .
09/25/14
20140288289
 Method for detecting and quantifying wheat endogenous gene patent thumbnailMethod for detecting and quantifying wheat endogenous gene
Provided is a method of detecting or quantifying a wheat species-specific dna in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having at partial sequence of a nucleotide sequence identified as seq id no: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule..
09/25/14
20140287420
Microfluidics polymerase chain reaction and high resolution melt detection
The present invention relates to a method and system for polymerase chain reaction (“pcr”), high resolution melt (“hrm”) analysis and microfluidics, and, more specifically, to a method and system for implementing the processes of pcr and hrm on a microscale in a microfluidics chamber for certain purposes including for purposes of dna detection and/or extraction.. .
09/25/14
20140287405
Method for detecting and quantifying wheat endogenous gene
Provided is a method of detecting or quantifying a wheat species-specific dna in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having a partial sequence of a nucleotide sequence identified as seq id no: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule..
09/18/14
20140273185
Tactical and portable pcr/hrm genome identification system
The present invention relates to a polymerase chain reaction and high resolution melt genetic identification system, and, more specifically, to a tactical and portable polymerase chain reaction and high resolution melt genetic analysis and identification system that is configured to determine and communicate analysis and identification results and a tiered confidence/alert level related to the analysis and identification results.. .
09/18/14
20140273099
Methods and devices for non-therman polymerase chain reaction
Methods and devices for non-thermal pcr amplification of nucleic acid sequences. An electrical potential is applied to cause non-thermal separation of strands of a double-stranded nucleic acid or double-stranded nucleic acid/primer extension product..
09/18/14
20140272998
Diagnostic microrna profiling in cutaneous t-cell lymphoma (ctcl)
The present invention relates to the field of cancer-diagnostics. In particular the invention relates to a microrna expression signature that allows discriminating skin samples of cutaneous t-cell lymphomas (ctcl) from non-malignant (inflammantory) skin samples by use of quantitative polymerase chain reaction performed on reverse transcribed mirna.
09/18/14
20140272984
Microfluidic flow monitoring
The present invention relates to systems and methods of monitoring velocity or flow in channels, especially in microfluidic channels. In some embodiments, the present invention relates to systems and methods of monitoring velocity or flow rate in systems and methods for performing a real-time polymerase chain reaction (pcr) in a continuous-flow microfluidic system..
09/18/14
20140272977
Molecular targets and methods for formulation screening and preservative efficacy testing
Methods and kits are disclosed for distinguishing viable from nonviable microbial cells. The methods and kits are useful in the screening of cell culture formulations and the testing of preservative efficacy.
09/18/14
20140272927
Methods, devices, and systems for processing multiple assays background
Methods, devices, and systems for performing polymerase chain reaction (pcr) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both pcr temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the pcr temperature cycling for real-time pcr and/or during temperature ramping in the melt data acquisition.
09/04/14
20140249142
Discovery of a somatic mutation in myd88 gene in lymphoplasmacytic lymphoma
Diagnostic assays for facilitating the diagnosis of lymphoplasmacytic lymphoma (lpl) are provided. The method comprises assessing a biological sample of the subject for the presence of a mutation at position 38182641 in chromosome 3p22.2, wherein presence of the mutation is indicative that the subject has lpl.
09/04/14
20140248711
Apparatus and method for electrical detection of oligonucleotides through pore blockades
Systems and methods for specific nucleic acid (na) sequence detection that do not rely on polymerase chain reaction (pcr) for target sequence amplification and do not require any special reagents other than a complementary sequence capture probe conjugated to spherical beads.. .
09/04/14
20140248622
Telomere length measurement in formalin-fixed, paraffin embedded (ffpe) samples by quantitative pcr
Methods of reliably quantifying telomere length in cells or tissues that have been formalin fixed and paraffin embedded (ffpe) samples by quantitative polymerase chain reaction protocol and kits for use with such various methods are provided. The methods of the present invention may be used to predetermine an individual's response to treatment with a telomerase inhibitor, a telomere damaging agent or a telomerase activator..
09/04/14
20140248613
Automatic detection kit for detecting hla alleles using real-time polymerase chain reaction
Provided is an automatic detection kit for automatically detecting hla alleles using a real-time polymerase chain reaction (pcr). The real-time pcr is performed on dna isolated from a sample using a primer which is able to specifically bind to hla alleles and a fluorescent probe which is able to detect amplification of the hla alleles in real time, and the hla allele typing is performed by analyzing a fluorescence value obtained from the real-time pcr using an hla automatic typing..
08/28/14
20140243242
Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a single reaction method to co-amplify multiple subsequences of a nucleic acid fragment sequence (i.e., for example, at least two read pairs from a single library insert sequence).
08/28/14
20140242594
Methods and compositions for preparation of nucleic acids
A method for isolating genomic dna (gdna) from a biological material. In some embodiments, the method includes (a) contacting a sample that contains gdna with a solution of hydroxide and a detergent under conditions and for a time sufficient to degrade a cell wall, a cell membrane, a nuclear membrane, a nucleoprotein, or combinations thereof and/or to denature the gdna; (b) mixing into the solution resulting from step (a) a solution characterized by high salt and sufficient buffering capacity to reduce the ph of the solution to less than 10, thereby producing a neutralized solution; (c) centrifuging the sample at a speed and length of time sufficient to clarify the preparation; and (d) removing insoluble material from the neutralized and clarified preparation, whereby a solution of gdna is produced.
08/21/14
20140236496
Methods and systems for visualizing and evaluating data
A computer-implemented method of generating a digital polymerase chain reaction (dpcr) result is provided. The method includes detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period.
08/21/14
20140235464
Detection and quantification of biomolecules using mass spectrometry
The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry.
08/21/14
20140234850
Dna fragment detection method, dna fragment detection kit and the use thereof
The disclosure claims a cleaved deoxyribonucleic acid (dna) detection method, a dna fragment detection kit and use thereof. Wherein, the method includes the steps of: designing primers according to a test site or a test region of the dna fragment; cyclizing the dna fragment to obtain acyclized dna; implementing polymerase chain reaction (pcr) amplification for the cyclized dna by using the primers; and detecting the pcr amplification product.
08/14/14
20140228256
Method and kit for constructing plasma dna sequencing library
The disclosure relates a method and a kit for constructing a plasma deoxyribonucleic acid (dna) sequencing library. The method provided by the disclosure includes: extracting a plasma dna; making the plasma dna ligate to a sequencing linker, and purifying a ligation product; performing polymerase chain reaction (pcr) amplification for the purified ligation product, purifying the pcr amplification product, and obtaining the plasma dna sequencing library, wherein, the method does not include the step of performing 5′-terminus phosphorylation for the plasma dna.
08/14/14
20140228223
High throughput paired-end sequencing of large-insert clone libraries
The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a paired end sequencing method that improves the yield of long-distance genomic read pairs by constructing long-insert clone libraries (i.e., for example, a fosill library or a foscn library) and converting the long-insert clone library using inverse polymerase chain reaction amplification or shearing and recircularization of shortened fragments into a library of co-ligated clone-insert ends.
07/31/14
20140213485
Methods for preparing cdna from low quantities of cells
Methods for preparing cdna libraries from single and low quantities of cells are disclosed. The methods are based on the principles of multi-strand displacement amplification or semi-random primed polymerase chain reaction.
07/31/14
20140212884
Composition and methods for rt-pcr comprising an anionic polymer
The present invention is in the fields of molecular biology. The present invention is directed to novel compositions, methods and kits useful for the generation of nucleic acids from an rna template and further nucleic acid replication.
07/24/14
20140206855
Methods and compositions for detecting cancers associated with methylation of hmlh1 promoter dna
Methods are provided for detection of cancers associated with methylation of hmlh1 promoter dna in a subject. The method comprise assaying for the presence of methylated hmlh1 promoter dna in a bodily fluid from a subject.
07/17/14
20140200167
Functionally integrated device for multiplex genetic identification
A biochip for multiplex genetic identification is disclosed. An biochip for separating and detecting a plurality of dna fragments includes a set of inputs and chambers for receiving a sample matrix of genetic material and reagents needed to conduct a polymerase chain reaction amplification of the genetic material.
07/17/14
20140199731
Assay and other reactions involving droplets
The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel.
07/17/14
20140199730
Assays and other reactions involving droplets
The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel.
07/03/14
20140186828
Methods for determining cell viability using molecular nucleic acid-based techniques
The present invention relates to novel methods, and kits, for selectively excluding dead cells from a mixture containing live and dead cells, such as microbe cells in clinical samples, blood products, medical/biotechnology products and food products where subsequent interrogation of the selected live cells are an indicator of the presence of microbe viability. In particular, the invention relates to improved methods for performing direct nucleic acid amplification techniques such as polymerase chain reaction (pcr) and isothermal techniques in blood and other body fluids, for correlation with microbe cell viability from bacteremia and fungemia samples.
06/26/14
20140178880
Method of preparing a reaction mixture and related products
The invention relates to a method of preparing a reaction mixture for polymerase chain reaction (pcr) assay and a solution set for pcr. The method comprises providing a sample solution comprising a biological sample to be amplified in said pcr assay and first colorant providing the solution a first color, providing a reagent solution comprising at least one other substance required for performing said assay and second colorant providing the solution a second color different from the first color, and mixing the sample solution and the first reagent solution for providing a mixed solution to be subjected to the pcr process, the mixed solution having, due to said first and second colorants, a third color different from the first and second colors.
06/19/14
20140171334
Gene analysis method using sdl-pcr
According to the sdl-pcr method of the present invention, non-specific amplification can be minimized by removing non-ligated probes or genomic dna using a tag, and separation can be achieved within a shorter time compared to a separation method that is performed using exonuclease. In addition, ligation, separation and polymerase chain reaction processes can be performed in a single solution in a single tube, and thus a plurality of genes can be amplified at the same time in an accurate and rapid manner..
06/19/14
20140170706
Low-mass sample block with rapid response to temperature change
A sample block for use in the polymerase chain reaction, dna sequencing, and other procedures that involve the performance of simultaneous reactions in multiple samples with temperature control by heating or cooling elements contacting the bottom surface of the block is improved by the inclusion of hollows in the block that are positioned to decrease the mass of the block in the immediate vicinity of the wells.. .
06/12/14
20140162321
Preparation of gene-specific templates for the use in single primer amplification
This disclosure relates to methods for creating engineered templates that are useful for amplification of one or more antibody genes without the use of gene-specific primers. More specifically, templates engineered using these methods in a polymerase chain reaction setting which allows for the specific amplification of one or more antibody genes..
06/12/14
20140162267
Dry composition of reaction compounds with stabilized polymerase
The present invention provides methods to obtain dry compositions of reaction compounds that maintain the biological activity of the compounds upon re-solubilization after a certain storage time. Preferably, the dry composition comprises a polymerase, and the dry composition is usable for polymerase chain reaction (pcr) amplification after re-solubilization..
06/12/14
20140162266
Methods for polymerase chain reaction copy number variation assays
This disclosure provides methods for measuring the copy number for highly amplified and/or abundant genomic loci. Recognized herein is a need for methods for determining nucleic acid copy number, particularly in instances where one locus to be quantified (i.e., the target) is relatively more abundant than a locus of known abundance (i.e., the reference).
06/12/14
20140162261
Detection kit for identifying genotype in depression patients and method of using the same
The present invention relates to a rs6311 test kit, which includes a probe, a primer, and a polymerase chain reaction solution, wherein said probe sequence is as follows: rs6311t-fam: ctgtgagtgtctggc (seq. Id.
06/05/14
20140154681
Methods to predict breast cancer outcome
The present invention provides methods for predicting the outcome of a subject having breast cancer. The prediction model is based on the gene expression profile of the genes listed in table 1a or 1b.
05/29/14
20140147849
Quantitation of human genomic and mitochondrial dna
Methods are provided for determining, in a single polymerase chain reaction (pcr) reaction, the quantity, quality, and gender of origin of dna in a sample, and whether or not the sample contains pcr amplification inhibitors. The methods involve carrying out a single pcr multiplex reaction utilizing primer sets specific for amplifying: the human amelogenin locus; an x- and/or y-chromosome specific gene that is shorter than the amelogenin gene; at least one mitochondrial dna sequence, and preferably two differently-sized mitochondrial sequences; and a heterologous, non-human reporter gene..
05/22/14
20140141419
Single tube quantitative polymerase chain reaction (pcr)
Provided herein are systems and methods for quantitatively monitoring target amplicons produced by polymerase chain reaction (pcr). In particular, quantitative monitoring of target amplicon(s) in a single-tube pcr reaction without separate calibration reactions are provided..
05/15/14
20140134633
Detergent free polymerases
The present invention relates to a formulation of a thermostable dna polymerase which is completely free of detergents and its particular use in real time polymerase chain reaction (pcr). Such a formulation may be obtained if the selected purification method does not require the addition of a detergent at any purification step..
05/08/14
20140127702
Polymerization of nucleic acids using proteins having low isoelectric points
This disclosure relates to the use of one or more proteins (e.g., globular proteins) having a low isoelectric point and/or a limited number (e.g., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (pcr).. .
04/24/14
20140113299
Inhibition-resistant polymerases
Provided herein are mutant polymerase enzymes resistant to inhibitors encountered in polymerase chain reactions (pcr). Also provided are nucleic acids or constructs encoding isolated polypeptides having polymerase activity.
04/10/14
20140099644
Novel compositions, methods and kits for real time polymerase chain reaction (pcr)
The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.. .
04/10/14
20140099636
Field-based qpcr microbial monitoring
Dna/rna monitoring of microbes at an oil field to determine the presence and activity of harmful microbes is accomplished with a portable qpcr (quantitative polymerase chain reaction) machine. This permits the monitoring to occur on-site in the field and reduces the variability that may occur from the transportation of samples..
04/03/14
20140093942
Gene, ars-r anchorage cassette, ars-r expression-anchorage cassette, recombinant plasmid, bacterial transgenic lineage, use of said gene, use of said lineage in environmental bioremediation processes
The present invention relates to the construction and insertion of a dna plasmid vector of broad spectrum for gram-negative bacteria, that carries a gene sequence which, when expressed, enables the anchorage of a chelator protein for arsenic ions on the gram-negative bacteria cellular surface. For that end, the structural sequence of the regulatory arsr gene without stop codon (seq id no 1) was amplified by polymerase chain reaction (pcr) using as a template the chromosome 1 of cupriavidus metallidurans, ch34 lineage and inserted into the pgem-t cloning vector, yielding the pgemt-as plasmid (seq id no 2).
04/03/14
20140093872
Method of mutation detection in blood cell-free dna using primer extension (pe) and pcr
A method of detecting mutation in blood cell-free dna, includes providing a serum sample, isolating dna from the serum sample, amplifying the dna by polymerase chain reaction (pcr), subjecting the pcr product to primer extension (pe), and separating the pe reaction product and identifying the mutation by gel electrophoresis. In order to improve accuracy and sensitivity, the pe reaction can be carried out by using a primer that blocks the extension of the wild or non-mutated sequence..
03/20/14
20140080133
Thermally controlled chamber with optical access for high-performance pcr
Novel methods and systems for polymerase chain reaction (pcr) are disclosed. A pcr device has a bottom heater layer, a central reacting layer, and a top heater layer.
03/06/14
20140066322
Mouse cell line authentication
A multiplex polymerase chain reaction assay that targets nine tetranucleotide short tandem repeat (str) markers in the mouse genome. Unique profiles were obtained from seventy-two mouse samples that were used to determine the allele distribution for each str marker.
03/06/14
20140065702
Polymerase chain reaction
A polymerase chain reaction (pcr) device for a reverse transcriptase reaction (rt) and a convectively-driven polymerase chain reaction (cpcr) in the same device is provided. The pcr device comprises an upper temperature-controlling unit, a middle temperature-controlling unit and a lower temperature-controlling unit.
03/06/14
20140065609
Varietal counting of nucleic acids for obtaining genomic copy number information
A method for obtaining from genomic material genomic copy number information unaffected by amplification distortion, comprising obtaining segments of the genomic material, tagging the segments with substantially unique tags to generate tagged nucleic acid molecules, such that each tagged nucleic acid molecule comprises one segment of the genomic material and a tag, subjecting the tagged nucleic acid molecules to polymerase chain reaction (pcr) amplification, generating tag associated sequence reads by sequencing the product of the pcr reaction, assigning each tagged nucleic acid molecule to a location on a genome associated with the genomic material by mapping the subsequence of each tag associated sequence read corresponding to a segment of the genomic material to a location on the genome, and counting the number of tagged nucleic acid molecules assigned to the same location on the genome having a different tag, thereby obtaining genomic copy number information unaffected by amplification distortion.. .
03/06/14
20140065603
Quantitative real-time assay for noroviruses and enteroviruses with built in quality control standard
A method is provided for reverse transcription-polymerase chain reaction (rt-pcr) comprising a) amplifying a reverse transcribed cdna in a mixture comprising norovirus genogroup i and norovirus genogroup ii primers and probes, wherein said norovirus primers and probes distinguish between genogroup i and genogroup ii viruses; b) quantifying virus; and c) normalizing data based on a universal internal rna control. Optionally, the method may also include primers and probes for enteroviruses.
03/06/14
20140065602
Human exhaled aerosol droplet biomarker system and method
A system and method for detecting a biomarker in exhaled breath condensate nanodroplets comprises noninvasively collecting exhaled breath condensate nanodroplets of a subject, and analyzing said nanodroplets utilizing immuno-quantitative polymerase chain reaction to detect one or more target biomarkers.. .
02/27/14
20140057863
Modified nucleosides for the treatment of viral infections and abnormal cellular proliferation
This invention also provides an effective process to quantify the viral load, and in particular bvdv, hcv or west nile virus load, in a host, using real-time polymerase chain reaction (“rt-pcr”). Additionally, the invention discloses probe molecules that can fluoresce proportionally to the amount of virus present in a sample..
02/27/14
20140057279
Devices and methods for biological sample-to-answer and analysis
Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk.
02/27/14
20140057210
Methods of fabrication of cartridges for biological analysis
Methods to fabricate reaction cartridges for biological sample preparation and analysis are disclosed. A cartridge may have a reaction chamber and openings to allow fluids to enter the chamber.
02/20/14
20140051159
Micro-fluidic modules on a chip for diagnostic applications
A chip having a substrate for amplifying genetic material includes a substrate heater having a plurality of substrate heating resistors. The heating resistors define a first temperature zone for maintaining a first temperature, a second temperature zone for maintaining a second temperature below the first temperature, and a third temperature zone for maintaining a third temperature between the first and second temperatures, on the substrate.
02/20/14
20140051155
Thermal cycler apparatus and related methods
An apparatus for thermal cycling can transfer heat uniformly and efficiently. The apparatus can be used in a method that reduces condensation on sample wells.
02/13/14
20140045250
Real-time optical system for polymerase chain reaction
An improved device and system for facilitating polymerase chain reaction including a light source, detector, waveguide, and filters that occupy minimal space and facilitate reduced sample read time and rapid reading of multiple light wavelengths.. .
02/13/14
20140045190
Method and device for monitoring real-time polymerase chain reaction (pcr) utilizing electro-active hydrolysis probe (e-tag probe)
A method for real-time electrochemical monitoring of pcr amplicons using a hydrolysis probe that is labeled with electro-active indicators and a microchip for implementing the method. The method provided is simpler and has higher specificity compared with the prior art.
02/06/14
20140038896
Retroelements and mental disorders and methods of measuring l1 retrotransposition
A method of treating increased non-ltr retrotransposition in a cell. The method includes exposing a neural cell to a retrotransposition inhibitor in an amount sufficient to decrease the non-ltr retrotransposition in the neural cell or a progeny of the neural cell.
01/30/14
20140031241
Paired end bead amplification and high throughput sequencing
The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a paired end sequencing method that enables the sequencing of unique read pairs by co-localizing both 5′ ends on a single emulsion polymerase chain reaction bead.


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Polymerase Chain Reaction topics: Polymerase Chain Reaction, Polymerase, Nucleic Acid, Nucleotide, Quantitative, Amplification, Chlamydia Trachomatis, Nucleic Acids, Transcription, Oligonucleotide, Sequencing, Real Time Pcr, Differentiation, Lymphogranuloma Venereum, Recombinant

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