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Polymerase Chain Reaction

Polymerase Chain Reaction-related patent applications - as published by the U.S. Patent and Trademark Office (USPTO).


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Test kits and methods for their use to detect genetic markers for transitional cell carcinoma ...
Pacific Edge Limited
April 05, 2018 - N°20180094323

This invention relates to methods for detecting the presence of genetic markers for transitional cell carcinoma of the bladder. Specifically, this invention relates to detection of expression of overexpressed and underexpressed genetic markers and calculation of ratios of expression of these markers. In additional aspects, the invention relates to polymerase chain reaction (pcr) based kits for carrying out these methods.
Method for determining genetic condition of fetus
Fujifilm Corporation
March 29, 2018 - N°20180087112

Provided is a method for determining a genetic condition of a fetus, the method including: an objective region selection step of selecting an objective region for determining the genetic condition, from regions on a human genome; a step of isolating a single cell from a maternal blood sample; a step of extracting genomic dna from the single cell; a step ...
Method for reducing quantification errors caused by reaction volume deviations in digital polymerase chain reaction
Roche Molecular System, Inc.
March 29, 2018 - N°20180087090

The present disclosure relates to a method for reducing quantification errors caused by reaction volume deviations in digital polymerase chain reaction (dpcr) and to a method for determining the amount or concentration of a nucleic acid of interest in a sample with dpcr.
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Microfluidic siphoning array for nucleic acid quantification
Combinati Incorporated
March 22, 2018 - N°20180078935

In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides ...
Methods and compositions for identifying and quantifying microbial dna
Advatect Diagnostics, Llc
March 15, 2018 - N°20180073086

This invention relates to methods and compositions for identifying microbial dna in the tissues or body fluid samples of patients. More particularly, the invention relates to two-step polymerase chain reaction based methods for identifying microbial dna in the tissues or body fluid samples of patients, and compositions therefor. Microbial dna can also be quantified using the methods described herein.
Dumbbell-structure oligonucleotide, nucleic acid amplification primer comprising same, and nucleic acid amplification method using same
Diogene Co., Ltd
March 15, 2018 - N°20180073082

The present invention relates to a dumbbell-structure oligonucleotide (dso), a nucleic acid amplification primer comprising the same, and a nucleic acid amplification method using the same and, more specifically, to a method for multi-gene amplification and single-nucleotide polymorphism analysis using a dumbbell-structure oligonucleotide capable of excluding non-specific amplification products prior to binding to a template in a first cycle in ...
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Reaction mixtures
Roche Molecular Systems, Inc.
March 15, 2018 - N°20180073008

The present invention provides stable dried reaction mixtures, methods for their preparation, methods for their use, and kits comprising them. The stable dried reaction mixtures are useful in many recombinant dna techniques, especially nucleic acid amplification by the polymerase chain reaction (pcr).
Devices and methods for molecular diagnostic testing
Click Diagnostics, Inc.
March 15, 2018 - N°20180071734

A hand-held molecular diagnostic test device includes a housing, an amplification (or pcr) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (pcr) on the input sample. The detection module is configured ...
Pressurizable cartridge for polymerase chain reactions
Dxna Llc
March 15, 2018 - N°20180071733

A sample processing module includes a pcr assembly including at least one pcr reaction vial. A sample assembly is configured to hold a fluid sample therein, said sample assembly being attachable to said pcr assembly so as to form a closed system. A delivery channel is formed in said pcr assembly, said delivery channel being in fluidic communication with said ...
Competitive probes for engineering signal generation
Chromacode, Inc.
March 01, 2018 - N°20180057864

A method of identifying a first target nucleic acid comprising, providing a sample comprising the first target nucleic acid, providing a first set of paired oligonucleotides with complementarity to the first target nucleic acid, the first set of paired oligonucleotides comprising a first ratio of (a) first competitive oligonucleotides to (b) first signal oligonucleotides comprising a signal tag, wherein the ...
Sequence, technique platform, and method for in vitro detecting clostridium difficile ribotype 027
National Cheng Kung University
March 01, 2018 - N°20180057861

The invention relates to a sequence, a technique platform, and a method for in vitro detecting clostridium difficile ribotype 027. The technology platform includes a pair of primers specific to c. Difficile ribotype 027 and used for polymerase chain reaction (pcr), and primer sets as well as materials demanded for multiplex-pcr. The method comprises steps of obtaining a specimen dna, in vitro ...
Process using zen hydrolysis probe for detection of porcine contamination and a kit thereof
Universiti Brunei Darussalam
February 08, 2018 - N°20180037959

A rapid, sensitive and cost-effective process of detection of porcine contamination in a food product sample is provided herein. The present invention further provides a real-time polymerase chain reaction (pcr) based process which uses highly specific oligonucleotides primers and zen™ probe, and a kit that contains the primers and/or probe useful for rapid detection of porcine dna in ...
Method for quantitative monitoring of endospores in aqueous environment of a paper or board mill
Kemira Oyj
February 08, 2018 - N°20180037936

The invention relates to a method for quantitative monitoring of bacterial endospores in an aqueous environment of a paper or board mill. The method comprises at least the following steps: obtaining at least a first aqueous sample originating from the industrial aqueous environment; destroying bacteria in vegetative form in the first sample by a suitable treatment, preferably by heating the ...
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Methods and apparatuses for processing blood and other biological samples
Covaris, Inc.
February 08, 2018 - N°20180037883

Systems and methods for the high yield extraction and recovery of higher quality target molecule(s) (e. G., dna, rna, protein, lipids, metabolites) from blood spots. High quality dna recovered from dried blood spots can be an input source for high throughput analytical methods, such as for polymerase chain reaction (e. G., qpcr) and/or next generation sequencing (ngs). In ...
Method for designing primer used for polymerase chain reaction and primer set
Fujifilm Corporation
February 01, 2018 - N°20180032669

Provided is a method for designing a primer used for a polymerase chain reaction including: a local alignment step of obtaining a local alignment score by performing pairwise local alignment on a base sequence of a primer candidate under a condition that a partial sequence to be subjected to comparison includes the 3′ terminal of the base sequence of the ...
Methods and compositions for rapid multiplex application of str loci
Ande Corporation
February 01, 2018 - N°20180031481

Provided are methods for multiplex polymerase chain reaction (pcr) amplification of short tandem repeat (str) loci that can be used to rapidly generate a highly specific str profile from target nucleic acids. The resulting str profiles are useful for human identification purposes in law enforcement, homeland security, military, intelligence, and paternity testing applications.
Assay and other reactions involving droplets
President And Fellows Of Harvard College
January 25, 2018 - N°20180023109

The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be ...
Cutoff point delta ct method for genetic pcr testing in human cancer
Genetics Development Corporation
January 11, 2018 - N°20180010191

The present invention is related to an improved method for her2 gene test by using quantitative real-time pcr (polymerase chain reaction) technique. Our invention streamlines test process, and incorporates quality control for each major step, including sample, reagent, operation, and data report. We eliminate the need for reference genes which is hard to standardize in her2 pcr test. We develop ...
Highly sensitive method for detection of viral hiv dna remaining after antiretroviral therapy of aids ...
January 04, 2018 - N°20180003666

Methods for detecting polynucleotides, especially the dna replicated from samples obtained from subjects infected with pathogenic viruses such as human immunodeficiency virus, by detecting electromagnetic signals (“ems”) emitted by such polynucleotides, and methods for improving the sensitivity of the polymerase chain reaction (“pcr”).
Methods and compositions for identifying and quantifying microbial dna
Advatect Diagnostics, Llc
December 28, 2017 - N°20170369952

This invention relates to methods and compositions for identifying microbial dna in the tissues or body fluid samples of patients. More particularly, the invention relates to two-step polymerase chain reaction based methods for identifying microbial dna in the tissues or body fluid samples of patients, and compositions therefor. Microbial dna can also be quantified using the methods described herein.
Multiplex q-pcr arrays
California Institute Of Technology
December 21, 2017 - N°20170362648

This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (pcr) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in ...
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