|| List of recent Polymerase Chain Reaction-related patents
|Retroelements and mental disorders and methods of measuring l1 retrotransposition|
A method of treating increased non-ltr retrotransposition in a cell. The method includes exposing a neural cell to a retrotransposition inhibitor in an amount sufficient to decrease the non-ltr retrotransposition in the neural cell or a progeny of the neural cell.
|Hot-start digital pcr|
The present disclosure provides methods and compositions for performing nucleic acid reactions, such as hot-start digital polymerase chain reaction (dpcr).. .
|Step-up method for cold-pcr enrichment|
Methods of using polymerase chain reactions to enrich a target sequence in a sample containing reference sequences and target sequences having high homology and amplifiable by the same primer pair are provided herein. In particular the methods provide a robust means to improve the fold enrichment of the target sequence and minimize reaction-to-reaction, well-to-well and run-to-run variations in the enrichment methods..
|Nucleic acid-free thermostable enzymes and methods of production thereof|
The present invention provides thermostable enzymes, such as dna polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (pcr)..
|System for integrated analysis of real-time polymerase chain reaction and dna chip and method for integrated analysis using the same|
Provided are a system for integrated analysis of a real-time polymerase chain reaction and a dna chip and a method for integrated analysis using the same, and more particularly to an apparatus for integrated analysis of a real-time polymerase chain reaction and a dna chip and a method for integrated analysis using the same. According to the method for integrated analysis of a biomaterial of the present invention, gene amplification proceeds and subsequently hybridization proceeds in a single reactor, thereby preventing contamination of the sample due to external factors, which may be caused while the sample is transferred for reaction, and automating a series of procedures such as injection of the sample, reaction of the biomaterial, and detection and analysis of results..
|Pressurizable cartridge for polymerase chain reactions|
Methods and apparatus for use in connection with the performance of the polymerase chain reaction are provided. An exemplary sample processing module is described that includes a sample assembly and a pcr assembly, the sample processing module being configured to hold the sample therein at a pressure higher than ambient pressure.
|Methods and compositions for detecting serotypes of chlamydia trachomatis capable of causing lymphogranuloma venereum|
Disclosed are methods and compositions for conducting assays utilizing real-time polymerase chain reactions (“pcrs”) in detection of serotypes l i, l ii, and l iii, but not stereotype b, of chlamydia trachomatis, capable of causing lymphogranuloma venereum (“lgv”). These assays take advantage of a deletion occurring in the cytotoxin gene locus specific to the l i, l ii, and l iii serotypes.
|Method for detection, differentiation and quantification of t cell populations by way of reverse transcription quantitative real time pcr (rt-qpcr) technology|
The present invention relates to a method for detection, differentiation and quantification of t cell populations, comprising the following steps a) contacting a first aliquot of a body fluid of an individual with at least one antigen, wherein the body fluid contains antigen presenting cells (apc) and t cells, b) incubating the first aliquot with at least one antigen for a certain period of time, c) detection and differentiation of the t cell population by detecting in the first aliquot and in a second aliquot of the body fluid of the individual, which has not been incubated with the at least one antigen, at least a first marker of the apc induced by t cells in a specific t cell population using reverse transcription quantitative real time-time polymerase chain reaction (rt-qpcr), and d) detection and quantification of the t cell population by determining the ratio of the detected marker of the apc of the first aliquot to the second aliquot as well as a kit for performing the method.. .
|Methods and systems for processing data|
The present invention is directed to methods and systems for applications relating to correction of numerical data resulting from dynamic changes to a true value. Such methods and systems may be used in accurate and unbiased quantitative polymerase chain reaction measurement..
|Assay for chlamydia trachomatis by amplification and detection of chlamydia trachomatis pmpa gene|
A region of the chlamydia trachomatis pmpa gene has been identified which is useful for performing amplification assays to determine specifically whether c. Trachomatis is present in the sample being tested.
|Ndm-1 polymerase chain reaction (pcr) assay|
Provided herein are compositions, methods, and kits for detection, identification, and analysis of ndm-1 variant nucleic acid. In particular, provided herein are kits, compositions, and methods for the detection, identification, and analysis of the ndm-1 variant nucleic acid and bacteria o other organisms carrying the ndm-1 variant nucleic acid..
|Apparatus and methods for integrated sample preparation, reaction and detection|
Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided, as are methods for using such cartridges. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction.
|Detection of fusobacterium in a gastrointestinal sample to diagnose gastrointestinal cancer|
Fusobacterium is a genus of gram-negative, filamentous, anaerobic bacteria found as normal flora in the mouth and large bowel, and often in necrotic tissue. A comparison of microbial ribonucleic acids (rna) between colorectal carcinoma (crc) tissue and adjacent normal control tissue found the over-representation of f.
|Polymerase chain reaction detection system|
The present invention relates to methods and kits for nucleic acid detection in an assay system.. .
|Compositions and methods for cdna synthesis|
Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of rna into cdna and rt pcr without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cdna synthesis or nucleic acid amplification (e.g., by the polymerase chain reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications..
|Methods for high level multiplexed polymerase chain reactions and homogeneous mass extension reactions|
Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid..
|Oligonucleotides useful in methods for detecting and characterizing aspergillus fumigatus|
Methods for using oligonucleotides in the detection of aspergillus fumigatus are disclosed. The oligonucleotides of the invention have nucleotide sequences derived from the gene encoding the cytochrome p450 14 alpha-sterol demethylase (the cyp51a protein) of aspergillus fumigatus.
|Novel paramyxovirus and uses thereof|
Described herein are isolated paramyxovirus, a morbillivirus (fmopv), isolated nucleic acids encoding the genome of fmopv, isolated amino acid sequences of fmopv proteins, antibodies to fmopv and its proteins, and uses thereof. In certain embodiments, the modified fmopv is a feline morbillivirus.
|Reactivity-dependent and interaction-dependent pcr|
Methods, reagents, compositions, and kits for reactivity-dependent polymerase chain reaction (rd-pcr) and interaction-dependent polymerase chain reaction (id-pcr) are provided herein. Rd-pcr is a technique useful for determining whether a reactive moiety can form a covalent bond to a target reactive moiety, for example, in screening a library of candidate reactive moieties for reactivity with a target reactive moiety, and in identifying an enzyme substrate, for example, in protease substrate profiling.
|Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions|
The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (pcr) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence.
|Reaction tube for performing isothermal polymerase chain reaction therein|
A reaction tube for performing isothermal polymerase chain reaction therein is provided and includes an upper section, a lower capillary section, a linkage connecting the upper section and the lower capillary section, and a thermal conductor. The lower capillary section has an annealing portion, an annular heating groove, and a close end.
|Methods and compositions for performing nucleic acid amplification reactions|
The present disclosure provides methods and compositions for performing nucleic acid reactions, such as the reverse transcriptase-polymerase chain reaction (rt-pcr).. .
|Chimeric dna identifier|
Devices and methods for delivering a chimeric deoxyribonucleic acid (dna) marking agent to a target and identifying the target from the chimeric dna marking agent are provided. A delivery device may be a projectile, a spray canister or a wet/dry article.
|Personalized tumor biomarkers|
Clinical management of human cancer is dependent on the accurate monitoring of residual and recurrent tumors. We have developed a method, called personalized analysis of rearranged ends (pare), which can identify translocations in solid tumors.
|Pc board-based polymerase chain reaction systems, methods and materials|
An apparatus for performing a polymerase chain reaction (pcr) is disclosed. The apparatus comprises a pcr chamber for performing a polymerase chain reaction and a printed circuit board (pcb) fluidic device.
|Method for detecting mycobacterium tuberculosis and nontuberculous mycobacteria by using dual real-time polymerase chain reaction|
Disclosed are a primer set and/or a probe capable of detecting specific nucleotide sequences of mtc and ntm, a kit for the detection of mtc and ntm, comprising the same, and a method for detecting mtc and ntm by duplex real-time pcr using the same. Useful in detecting genes characteristic of mtc and ntm, the primer sets and/or probes, detection kits, and detection methods can be applied as the clinical diagnosis of diseases caused by mtc and ntm, and therefore find applications in the medical fields including hospitals, research institutes, etc..
|Method for cell lysis and pcr within the same reaction chamber|
A method for amplification of a target dna, comprising the steps of (i) transferring a liquid with a first volume comprising at least one or more living cells into a vessel (ii) adding to said vessel a pcr reaction buffer with a second volume, whereas said second volume is at least 2× as large as said first volume (iii) lysing said at least one or more living cells within said vessel by means of incubation for at least 1 minute at at least 90° c., and (iv) amplifying said target by means of a polymerase chain reaction without performance of an intermediate purification step.. .
|Detection of dna sequences as risk factors for hiv infection|
A method for identifying a risk factor for diseases, disorders or conditions, such as those caused by human immunodeficiency virus, using the polymerase chain reaction and specific primers. Methods for treating patients having these diseases, disorders or conditions by antimicrobial treatment of the risk factor by combined antiviral and antibacterial treatment or by sustaining or stimulating the subject's immune system.
|Methods for measuring enzyme activity useful in determining cell viability in non-purified samples|
The present invention relates generally to the field of detection of microorganisms, in particular detection of bacteria, to methods for measuring enzyme activity, such as dna polymerase activity, and particularly relates to such methods performed on microbial crude lysates, useful for determining microbial enzyme activities which can be linked to amplification signal generators such as real-time polymerase chain reaction (pcr) techniques, thereby enabling determination of microbial pathogens in samples such as unpurified blood and other body fluids. This invention also relates to reagents for use in such methods, and to test kits comprising such reagents for carrying out the methods..
|Sample container with sensor receptacle and methods of use|
Devices and methods are described herein that are configured for use in laboratory testing, such as, for example, during a procedure including the monitoring and detection of chemical reactions. For example, the systems and devices described herein can be used during a procedure to monitor and detect polymerase chain reactions (pcr).
|Methods and compositions for rapid multiplex amplification of str loci|
Provided are methods for multiplex polymerase chain reaction (pcr) amplification of short tandem repeat (str) loci that can be used to rapidly generate a highly specific str profile from target nucleic acids. The resulting str profiles are useful for human identification purposes in law enforcement, homeland security, military, intelligence, and paternity testing applications..
|Channels with cross-sectional thermal gradients|
Provided herein are systems, devices, and methods for generating thermal gradients in channels and uses thereof. In particular, provided herein are system, methods, and devices employing first and second thermal layers positioned around a channel in order to create a thermal gradient across a cross-section of the channel having, for example, a nucleic acid denaturation zone, a nucleic acid annealing zone, and a nucleic acid polymerization zone.
|Non-contact heating type of gene amplification system|
Disclosed is a non-contact heating type gene amplification system. The non-contact heating type gene amplification system includes separate light sources for each step of a polymerase chain reaction in order to perform a high-speed temperature cycle by moving a rotation unit, in which a plurality of sample chambers are formed, to places at which the sample chambers are controlled at the temperatures corresponding to each step..
|Method for detecting mycobacterium tuberculosis and nontuberculous mycobacteria using duplex polymerase chain reaction|
Disclosed are primer sets specific for the is6110 or 16s rrna gene characteristic of mtc and for the 16s rrna gene characteristic of ntm, kits for the detection of mtc and ntm, comprising the same, and methods for detecting mtc and ntm by duplex pcr using the same. Because the primers are exclusive to mycobacterium tuberculosis and nontuberculous mycobacteria, the methods, which can employ an inexpensive typical pcr cycler, can be a clinical diagnostic means useful for the detection of both mycobacterium tuberculosis and nontuberculous mycobacteria at the same time, with higher efficiency..
|Real-time pcr in micro-channels|
The present invention relates to methods for amplifying nucleic acids in micro-channels. More specifically, the present invention relates to methods for performing a real-time polymerase chain reaction (pcr) in a continuous-flow microfluidic system and to methods for monitoring real-time pcr in such systems..
|Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)|
The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (rt-pcr). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step rt-pcr procedure using combinations of reverse transcriptase and thermostable dna polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin.
|Method for detecting mycobacterium tuberculosis and nontuberculous mycobacteria using duplex real-time polymerase chain reaction and melting curve analysis|
Disclosed are primer sets specific for the is6110 gene characteristic of mtc and for the 16s rrna gene characteristic of ntm, kits for the detection of mtc and ntm, comprising the same, and methods for detecting mtc and ntm by duplex real-time pcr and melting profile analysis using the same. Because the primers are exclusive to mycobacterium tuberculosis and nontuberculous mycobacteria, the methods can be a clinical diagnostic means useful for the detection of both mycobacterium tuberculosis and nontuberculous mycobacteria at the same time, with higher efficiency..
|Microdroplet-manipulation systems and methods for automated execution of molecular biological protocols|
Disclosed herein are automated systems for performing various biochemical and molecular biological procedures, including processor-controlled execution of protocols involving multiple steps performed in, on, or with liquid microdroplets. Example protocols are the various polymerase chain reaction (pcr) protocols, but the subject systems are not limited to performing pcr protocols.
|Stable compositions for nucleic acid amplification and sequencing|
The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable dna polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing.
|Control of chemical reactions using isotachophoresis|
Isotachophoresis (itp) is exploited to control various aspects of chemical reactions. In a first aspect, at least one of the reactants of a chemical reaction is confined to an itp zone, but the resulting product of the chemical reaction is separated from this itp zone by the itp process.
|Method for detecting gene region features based on inter-alu polymerase chain reaction|
An array of inter-alu gene-enriched amplicons produced by a polymerase chain reaction (“pcr”) process. The pcr process comprises combining one or a plurality of head-type/tail-type alu- or aluy- or any other alu-subfamily-consensus sequence-based primer; a genomic dna template isolated from cells; and a pcr-extension mix.
|Highly sensitive method for detection of viral hiv dna remaining after antiretroviral therapy of aids patients|
Methods for detecting polynucleotides, especially the dna replicated from samples obtained from subjects infected with pathogenic viruses such as human immunodefiency virus, by detecting electromagnetic signals (“ems”) emitted by such polynucleotides, and methods for improving the sensitivity of the polymerase chain reaction (“pcr”).. .