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Polymerase Chain Reaction patents

      

This page is updated frequently with new Polymerase Chain Reaction-related patent applications.




 Method for steadying thermal convection flow field in solution during thermal convective polymerase chain reaction patent thumbnailnew patent Method for steadying thermal convection flow field in solution during thermal convective polymerase chain reaction
A method for steadying a thermal convection flow field in a pcr reaction solution during a thermal convective polymerase chain reaction (pcr) includes steps as follows. A pcr tube is provided.
Genereach Biotechnology Corp.


 Dry composition of reaction compounds with stabilized polymerase patent thumbnailDry composition of reaction compounds with stabilized polymerase
The present invention provides methods to obtain dry compositions of reaction compounds that maintain the biological activity of the compounds upon re-solubilization after a certain storage time. Preferably, the dry composition comprises a polymerase, and the dry composition is usable for polymerase chain reaction (pcr) amplification after re-solubilization..
Roche Molecular Systems, Inc.


 Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution patent thumbnailNon-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution
A multiplexed polymerase chain reaction (pcr) dna detection system and a method for dna detection within the pcr system are provided. The pcr dna detection system includes a color charge-coupled device (ccd) camera, fluorophore-quencher probes and an imaging chamber.
Agency For Science, Technology And Research


 Method of de-differentiating and re-differentiating somatic cells using rna patent thumbnailMethod of de-differentiating and re-differentiating somatic cells using rna
Rna prepared by in vitro transcription using a polymerase chain reaction (pcr)-generated template can be introduced into a cell to modulate cell activity. This method is useful in de-differentiating somatic cells to pluripotent, multipotent, or unipotent cells; re-differentiating stem cells into differentiated cells; or reprogramming of somatic cells to modulate cell activities such as metabolism.
Yale University


 Methods and devices for non-thermal polymerase chain reaction patent thumbnailMethods and devices for non-thermal polymerase chain reaction
Methods and devices for non-thermal pcr amplification of nucleic acid sequences. An electrical potential is applied to cause non-thermal separation of strands of a double-stranded nucleic acid or double-stranded nucleic acid/primer extension product..
The Regents Of The University Of California


 Porcine torque teno virus vaccines and diagnosis patent thumbnailPorcine torque teno virus vaccines and diagnosis
The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine torque teno virus (pttv) genotypes or subtypes pttv1a-va, pttv1b-va, pttv2b-va, and pttv2c-va. The present invention also provides infectious dna clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same.
Virginia Tech Intellectual Properties, Inc.


 Control of chemical reactions using isotachophoresis patent thumbnailControl of chemical reactions using isotachophoresis
Isotachophoresis (itp) is exploited to control various aspects of chemical reactions. In a first aspect, at least one of the reactants of a chemical reaction is confined to an itp zone, but the resulting product of the chemical reaction is separated from this itp zone by the itp process.
The Board Of Trustees Of The Leland Stanford Junior University


 Diagnostic methods for infectious disease using endogenous gene expression patent thumbnailDiagnostic methods for infectious disease using endogenous gene expression
Disclosed are methods of diagnosis of a pathogen-associated disease. These methods comprise: providing a biological sample from a human subject; determining presence, absence and/or quantity of a bacterial pathogen, a viral pathogen, or a combination thereof, by a pathogen culture, a serum antibody detection test, a pathogen antigen detection test, a pathogen dna and/or rna detection test, or a combination thereof; determining in the sample, expression levels of at least one endogenous gene in which aberrant expression levels are associated with infection with a pathogen, by a microarray hybridization assay, rna-seq assay, polymerase chain reaction assay, a lamp assay, a ligase chain reaction assay, a southern, northern, or western blot assay, an elisa or a combination thereof.
Washington University


 Devices for real-time polymerase chain reaction patent thumbnailDevices for real-time polymerase chain reaction
An improved device and system for facilitating polymerase chain reaction including a light source, detector, waveguide, and filters that occupy minimal space and facilitate detection of stationary samples, reduced sample read time, and simultaneous reading of multiple light wavelengths.. .
Streck, Inc.


 Step-up  cold-pcr enrichment patent thumbnailStep-up cold-pcr enrichment
Methods of using polymerase chain reactions to enrich a plurality of target sequences in a sample containing reference sequences and target sequences having high homology and amplifiable by the same primer pairs are provided herein. In particular the methods provide a robust means to improve the fold enrichment of the target sequences and minimize reaction-to-reaction, well-to-well and run-to-run variations in the enrichment methods, e.g., in multiplex reactions..

Variety identification-encoding system and encoding method using the same


Provided is a variety identification-encoding system, including: a chromosome-decoding module decoding a chromosome of a reference genome variety and a chromosome of a target variety; a variation region-detecting module detecting a variation region in the decoded chromosome through single nucleotide variation dense region analysis; an amplification result-acquiring module setting an indel marker in the detected variation region and amplifying the indel marker by a polymerase chain reaction (pcr) to acquire an amplification result; and an encoding module encoding the amplification result.. .
Republic Of Korea (mngmnt : Rural Devel. Admin.)


Devices and methods for molecular diagnostic testing


A hand-held molecular diagnostic test device includes a housing, an amplification (or pcr) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume.

Reactivity-dependent and interaction-dependent pcr


Methods, reagents, compositions, and kits for reactivity-dependent polymerase chain reaction (rd-pcr) and interaction-dependent polymerase chain reaction (id-pcr) are provided herein. Rd-pcr is a technique useful for determining whether a reactive moiety can form a covalent bond to a target reactive moiety, for example, in screening a library of candidate reactive moieties for reactivity with a target reactive moiety, and in identifying an enzyme substrate, for example, in protease substrate profiling.

Real time quantitative and qualitative analysis biosubstance


A method of quantitatively and qualitatively analyzing a biomaterial in real-time, the method comprising preparing a device for detecting a biomaterial, feeding a complex of first and second probes, a forward primer, a reverse primer, a sample comprising deoxynucleotide triphosphate, a polymerase having exonuclease activity, and a sample comprising target genes, and a reaction solution comprising a buffer into the reaction container, performing polymerase chain reaction comprising denaturation of the target genes in the sample, hybridization of the target genes, the complex, and the forward and reverse primers in the sample, and elongation of the primers through the polymerase having exonuclease activity, allowing for elongation of the second probe on the third probe by the polymerase after hybridizing the released second probe and the third probe fixed to the biochip, detecting a first fluorescence signal by the first phosphor and a second fluorescence signal by the second phosphor.. .

Apparatus for high throughput chemical reactions


Apparatus, systems, chips, and methods of performing a large number of simultaneous chemical reactions are provided herein. The chips of the invention comprise addressable units that can be addressed according to the temperature of the reaction to be run.

Instrument for monitoring polymerase chain reaction of dna


An optical instrument monitors pcr replication of dna in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded dna. A beam splitter passes an excitation beam to the vials to fluoresce the dye.

Instrument for monitoring polymerase chain reaction of dna


An optical instrument monitors pcr replication of dna in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded dna. A beam splitter passes an excitation beam to the vials to fluoresce the dye.

Rasal1 is a major tumor suppressor gene in thyroid cancer


The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for treating thyroid cancer.

Detection micro-rna with high specificity


The invention provides a method for detecting mirna based on polymerase chain reaction comprising evagreen dye, and the use of evagreen dye in elevating the binding specificity of primers to templates in pcr.. .

Reaction mixtures


The present invention provides stable dried reaction mixtures, methods for their preparation, methods for their use, and kits comprising them. The stable dried reaction mixtures are useful in many recombinant dna techniques, especially nucleic acid amplification by the polymerase chain reaction (pcr)..

Detailed assay protocol specification


Techniques are disclosed relating to assay configuration. Assay devices are often used to determine characteristics of test samples, e.g., using polymerase chain reaction (pcr) or deoxyribonucleic acid (dna) melt techniques.
Luminex Corporation


Gene expression profiles to predict breast cancer outcomes


Methods for classifying and for evaluating the prognosis of a subject having breast cancer are provided. The methods include prediction of breast cancer subtype using a supervised algorithm trained to stratify subjects on the basis of breast cancer intrinsic subtype.
British Columbia Cancer Agency Branch


Method for the quantitative analysis of nucleic acid fragmentation and amplificability


The present invention relates to a method for the quantitative analysis of complex nucleic acids (na), i.e. Their fragmentation/degradation and amplificability as a marker of biomolecular quality and integrity of a biosample.
Universität Heidelberg


Method for species identification by using molecular weights of nucleic acid cleavage fragments


A method for species identification by using molecular weights of nucleic acid cleavage fragments, comprising steps of: performing a polymerase chain reaction and a nucleic acid cleavage reaction to cleave the nucleic acid sequence of the to-be-identified species into multiple nucleic acid cleavage fragments having different molecular weights; measuring the molecular weights of the nucleic acid cleavage fragments by using a mass spectrometer; comparing the molecular weight of each nucleic acid cleavage fragments of the to-be-identified species with molecular weights of nucleic acid cleavage fragments of a known species in a database; determining a number n of the identical nucleic acid cleavage fragments between the two species; and calculating a ratio n/m of the number n to the total number m of the nucleic acid cleavage fragments of the known species, wherein the ratio n/m represents similarity between the to-be-identified species and the known species.. .
Tech-knowhow Corp.


Methods of performing polymerase chain reaction and related uses thereof


Methods and kits for performing polymerase chain reaction (pcr) and melting analyses to determine copy number variation and/or gene expression are disclosed herein. Related uses of such methods and analyses are also disclosed herein..

Gene expression profiles to predict breast cancer outcomes


Methods for classifying and for evaluating the prognosis of a subject having breast cancer are provided. The methods include prediction of breast cancer subtype using a supervised algorithm trained to stratify subjects on the basis of breast cancer intrinsic subtype.
British Columbia Cancer Agency Branch


Determination of variants produced upon replication or transcription of nucleic acid sequences


A method of determining whether or not a nucleic acid having an expected sequence or one or more variants of the expected sequence are present in a sample containing nucleic acids after replication, transcription or editing (or other transformation) of a substrate nucleic acid. The method involves deciding an expected sequence likely to be formed in the sample upon the replication, transcription or editing of the substrate nucleic acid, and possible variants of the expected sequence, providing primer pairs for a polymerase chain reaction, reverse transcriptase polymerase chain reaction or ligase chain reaction, carrying out the polymerase chain reaction or reverse transcriptase polymerase chain reaction in one or more steps to form amplicons, and analyzing the amplicons to determining whether or not a nucleic acid having the expected sequence and/or variants are present in the sample.
Bio-id Diagnostic Inc.


Novel genomic marker of metastatic prostate cancer


The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for assessing prostate cancer in a patient.
The Johns Hopkins University


Determination of methylation state and chromatin structure of target genetic loci


The subject invention pertains to a method of determining methylation state and chromatin structure of target loci. The method comprises treating the genetic material obtained from the cells with dna methyltransferase, capturing target genetic loci using a set of oligonucleotides, ligating the target loci with oligonucleotide patches that flank the target loci, treating the target loci flanked by oligonucleotide patches with bisulfite, optionally amplifying the target loci by polymerase chain reaction, sequencing the pcr products, and analyzing the sequences to determine methylation state and chromatin structure of the target loci.
University Of Florida Research Foundation, Inc.


System and methods for determination of temperature cycling protocols for polymerase chain reactions


In one aspect, methods are described herein for enhancing one or more nucleic acid interactions. For example, in some embodiments, methods of enhancing one or more steps of polymerase chain reaction (pcr) are described herein.

Methods for high level multiplexed polymerase chain reactions and homogeneous mass extension reactions


Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid..

In situ interaction determination


Methods, reagents, compositions, and kits for in situ interaction determination (isid) via interaction-dependent polymerase chain reaction (id-pcr) are provided herein. Isid technology is useful for rapidly evaluating potential small molecule-target interactions from mixtures in a single solution.
President And Fellows Of Harvard College


Application of thiolated single-stranded dna in polymerase chain reaction


An application of thiolated single-stranded dna enhances specific amplification of polymerase chain reaction, namely a method for enhancing specific amplification of polymerase chain reaction by utilizing thiolated single-stranded dna, which includes the following step: adding an appropriate amount of the thiolated single-stranded dna into a pcr system to perform pcr amplification, wherein the appropriate amount means that the final concentration of the thiolated single-stranded dna in a 20 μl reaction system is not less than 15 μm. The thiolated single-stranded dna meets the following conditions: the thiolated single-stranded dna is one segment of any sequence which is non-complementary and non-homologous to a target sequence; the tm value is not less than 37.7° c.; and at least one end contains a thiolalkyl group sh—c6h12—..
Institute Of Plant Protection, Shandong Academy Of Agricultural Sciences


Methods for pathogen detection and disease management on meats, plants, or plant parts


Provided are methods for detecting pathogens affecting meats, plants, or plant parts. Also provided are methods for predicting disease and/or disease management for meats, plants, or plant parts.
Agrofresh Inc.


Analysis analyzing a nucleic acid amplification reaction


An analysis method is provided aimed at improving the linearity (precision and/or accuracy) of a quantification by a real-time nucleic acid amplification reaction rnr such as a polymerase chain reaction pcr over a wide range of analyte concentrations and/or limiting the effects of the presence of interfering substances by way of determining a quantification cycle number (cq) of the rnr as the cycle number corresponding to an intersection of the growth curve with a combined threshold function (ctf) over the rnr cycle range comprising at least two different threshold levels, the quantification cycle number (cq) being indicative of a quantitative and/or qualitative analysis result of a growth curve, the growth curve being indicative of the intensity of the fluorescence emission of an analyte for each amplification reaction cycle of the rnr over a rnr cycle range.. .
Roche Molecular Systems, Inc.


Method and device for polymerase chain reaction


Method and apparatus for amplifying a target nucleic acid sequence of a reaction mixture in a polymerase chain reaction (pcr). The method includes contacting the reaction mixture with emr frequency absorbing particles formed from a material having a transition metal, transition metal oxide or a transition metal hydroxide, or a nitride, a phosphide or an arsenide of a group iii metal doped with the transition metal or a transition metal oxide, or silicon dioxide doped with the transition metal, transition metal oxide, or transition metal hydroxide; and irradiating the emr absorbing particles with emr having a frequency of about 200 khz to 500 thz to amplify the target nucleic acid sequence, wherein the group iii metal is any one of al, ga, and in, and the transition metal is any one of mn, fe, co and cu..
National Cheng Kung University


Identifying affinity-matured human antibodies


Antigen-specific immunoglobulin v-regions are identified from a library of nucleic acids amplified using polymerase chain reaction using leader sequence-specific forward primers. The use of leader sequence primers allows all v-region sequences to be amplified (including those with extensive 5′ end mutations) without loss of the original 5′ v gene segment sequence.
Xbiotech, Inc.


Identifying affinity-matured human antibodies


Antigen-specific immunoglobulin v-regions are identified from a library of nucleic acids amplified using polymerase chain reaction using leader sequence-specific forward primers. The use of leader sequence primers allows all v-region sequences to be amplified (including those with extensive 5′ end mutations) without loss of the original 5′ v gene segment sequence.
Xbiotech, Inc.


System and serial processing of multiple nucleic acid assays


The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (pcr) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided.
Canon U.s. Life Sciences, Inc.


Compositions and methods for cdna synthesis


Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of rna into cdna and rt pcr without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cdna synthesis or nucleic acid amplification (e.g., by the polymerase chain reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications..

Systems and methods for baseline correction using non-linear normalization


Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (pcr), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction.
Applied Biosystems, Llc


Method for relative quantification of changes in dna methylation, using combined nuclease, ligation, and polymerase reactions


The present invention is directed to methods for identifying the presence of one or more methylated or unmethylated target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction.
Cornell University


Application of biofilm formation inhibiting compounds enhances control of citrus canker


Citrus canker caused by the bacterium xanthomonas citri subsp. Citri (xac) is an economically important disease of citrus worldwide.
University Of Florida Research Foundation, Inc.


Detection of genomic rearrangements by sequence capture


Provided herein is a method of sample analysis. In some embodiments, the method comprises hybridizing fragmented genomic dna from a test genome with a population of first oligonucleotides of the formula v1-b-v2 in the presence of one or more second oligonucleotides; contacting the product with ligase to join the ends of the fragmented genomic dna that are hybridized to v1 and v2 to the one or more second oligonucleotides; and subjecting the product to polymerase chain reaction conditions using amplification primers that hybridize to sites that are provided by the one or more second oligonucleotides, wherein production of a product indicates that the test genome contains a chromosomal rearrangement relative to the reference genome..
Agilent Technologies, Inc.


Methods for one step nucleic acid amplification of non-eluted samples


The present invention relates to methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. For easy processing and amplification of nucleic acid samples, the samples are bound to a solid support and used directly, without purification, in a nucleic acid amplification reaction such as the polymerase chain reaction (pcr)..
Ge Healthcare Uk Limited


Polymerase chain reaction detection system


The present invention relates to methods and kits for nucleic acid detection in an assay system.. .
Lgc Genomics Ltd


Detection of dna sequences as risk factors for hiv infection


A method for identifying a risk factor for diseases, disorders or conditions, such as those caused by human immunodeficiency virus, using the polymerase chain reaction and specific primers. Methods for treating patients having these diseases, disorders or conditions by antimicrobial treatment of the risk factor by combined antiviral and antibacterial treatment or by sustaining or stimulating the subject's immune system.

Direct quantitative pcr absent minor groove binders


Disclosed herein are methods, compositions and kits for the quantification of a nucleic acid target present on a solid support. This entails quantitative real-time polymerase chain reaction wherein minor groove binders are excluded..
Life Technologies Corporation


Direct quantitative pcr absent minor groove binders


Disclosed herein are methods, compositions and kits for the quantification of a nucleic acid target present on a solid support. This entails quantitative real-time polymerase chain reaction wherein minor groove binders are excluded..
Life Technologies Corporation


Compositions and methods for inhibiting terminal transferase activity


The present invention realtes to systems and methods for amplifying nucleic acid. In particular, systems and methods are provided for inhibiting polymerase based terminal transferase activity within a polynucleotide amplification setting (e.g., polymerase chain reaction).
Ibis Biosciences, Inc.


Systems and methods for calibration using dye signal amplification


The present teachings relate to a method of generating calibration information during a real-time polymerase chain reaction (rt-pcr) or other amplification reaction. A sample well plate or other support can contain one or more dyes or other reference materials that are subjected to the same rt-pcr thermal cycles or other conditions used to conduct amplification or other reactions on a biological sample.
Applied Biosystems, Llc


Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms


A method for amplifying and detecting microorganisms, such as species of listeria, is described. The method utilizes gene-matched enrichment media and pcr-based detection.
Battelle Memorial Institute


Freeze-dried composition


The invention relates to the use of a polysaccharide having at least four saccharide units, such as stachyose, as a glass-forming agent for the freeze-drying of a reaction mixture comprising an enzyme. In particular, the enzyme is a polymerase useful in a nucleic acid amplification reaction such as a polymerase chain reaction.
Fluorogenics Ltd


Assay and other reactions involving droplets


The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel.
President And Fellows Of Harvard College


Borrelia provocation procedure kit


The testing for the lyme disease pathogen, (borrelia bergdorferi (bb) and all other lyme borrelia), is notoriously difficult. Bb is not by nature a blood loving bacteria, and prefers the climate of avascular tissues such as cartilage.

Personalized tumor biomarkers


Clinical management of human cancer is dependent on the accurate monitoring of residual and recurrent tumors. We have developed a method, called personalized analysis of rearranged ends (pare), which can identify translocations in solid tumors.
The Johns Hopkins University


Polymerase chain reaction detection system


The present invention relates to methods and kits for nucleic acid detection in an assay system.. .
Lgc Genomics Limited


Instrument for monitoring polymerase chain reaction of dna


An optical instrument monitors pcr replication of dna in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded dna. A beam splitter passes an excitation beam to the vials to fluoresce the dye.
Life Technologies Corporation


Device, killing viruses in blood


A device, system and method for killing viruses in blood. An iontophoretic terminal delivery system includes a smart catheter, aleated electrode within the smart catheter, a terminal and an electrical system.

Kit comprising primers for amplifying alk kinase domain nucleic acids


Disclosed are methods and kits for detecting the presence of a cancer in a subject and assessing the efficacy of treatments for the same. The disclosed method use reverse transcription polymerase chain reaction (rt-pcr) techniques to detect the presence of point mutations, truncations, or fusions of anaplastic lymphoma kinase..
Insight Genetics, Inc.


Methods and systems for fast pcr heating


A microplate for polymerase chain reaction (pcr) comprises a substrate having a metallic material for heating pcr samples, and a barrier layer disposed adjacent to the substrate. In some cases, the barrier layer is formed of a first polymeric material.
Bjs Ip Ltd.


Quantitative reverse transcription polymerase chain reaction kit for breast cancer drug screening test and early diagnosis using tissue and blood


A method for a breast cancer drug screening including separating a full-length rna from a cell obtained from a tissue or blood of a suspected cancer patient; synthesizing a cdna from the full-length rna; performing a pcr with the synthesized cdna by using a composition including sets of a pair of primers and a probe, each set independently able to amplify human epidermal growth factor receptor 2 (her2), cytokeratin 19, epcam, a htert, ki67, vimentin, and a gapdh. The method further includes comparing an amount of the amplification with an expression amount to a normal person.
Yonsei University Wonju Industry-academic Cooperation Foundation


Primers, probes, and methods for mycobacterium tuberculosis specific diagnosis


Practical and rapid methods for the diagnosis of mycobacterium tuberculosis (mtb) using the polymerase chain reaction (pcr) have long been sought particularly because of the incidence of human immunodeficiency virus (hiv) coinfection and because standard culture methods are not practical given the long incubation periods needed and the growing prevalence of drug resistant strains.. .
Boston Medical Center Corporation


Method for determining the presence of diarrhoea causing pathogens


This invention relates to the field of detection of diarrhoea causing pathogens from patient, food or environmental samples. Particularly, the present invention provides a polymerase chain reaction (pcr) based assay method for detection of diarrhoea causing pathogens.
Mobidiag Oy


Methods for one step nucleic acid amplification of non-eluted samples


The present invention relates to methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. For easy processing and amplification of nucleic acid samples, the samples are bound to a solid support and used directly, without purification, in a nucleic acid amplification reaction such as the polymerase chain reaction (pcr)..
Ge Healthcare Uk Limited


Methods and kits for detection of a pathogen in sugarcane


Embodiments of the present invention provide a diagnostic approach utilizing quantitative polymerase chain reaction (pcr) to detect quantitatively a pathogen of the genus leifsonia that causes ratoon stunting disease (rsd) in sugarcane. This is a rapid, cost-effective and/or high sensitivity methodology for detecting this pathogen.

Reaction plate


A reaction plate includes a welded plastics planar laminate consisting of an aperture plate and a film, the aperture plate having at least one planar surface and a plurality of apertures in the planar surface of the apertured plate and the film being attached to the planar surface of the apertured plate around the or each aperture by welding. The welding is preferably laser or transmission welding.

Nucleic acid amplification controls and kits and methods of use thereof


The invention provides components and methods for polymerase chain reaction assays. The assays minimize both handling of material and time spent running samples.
Idexx Laboratories, Inc.


Pcr reaction mixtures and methods of using same


Provided herein are methods and compositions involving polymerase chain reaction (pcr).. .
Molecular Detection Israel Ltd.


Pcr assay for animal origin of heparin


The invention provides methods and reagents for use in distinguishing heparin sodium of porcine origin from heparin sodium of bovine origin using quantitative polymerase chain reaction.. .

Apparatus and methods for integrated sample preparation, reaction and detection


Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided, as are methods for using such cartridges. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction.
Luminex Corporation


Sample container with sensor receptable and methods of use


Devices and methods are described herein that are configured for use in laboratory testing, such as, for example, during a procedure including the monitoring and detection of chemical reactions. For example, the systems and devices described herein can be used during a procedure to monitor and detect polymerase chain reactions (pcr).
Heatflow Technologies Inc.


Methods, systems, and applications for solar-thermal microfluidic pcr


Disclosed are methods and apparatus for solar-thermal microfluidic polymerase chain reaction. A device comprises a microfluidic chip including at least one pcr region, an energy absorption layer disposed adjacent to the microfluidic chip, a solar energy concentrator adapted to produce a plurality of temperature profiles on the microfluidic chip adapted to facilitate pcr, and a photomask disposed between the solar energy concentrator and the microfluidic chip..
Cornell University


Methods and compositions relating to diagnosing and treating receptor tyrosine kinase related cancers


Disclosed are methods and compositions for detecting the presence of a cancer in a subject and assessing the efficacy of treatments for the same. The disclosed method use reverse transcription polymerase chain reaction (rt-pcr), real time polymerase chain reaction, and multiplex polymerase chain reaction techniques to detect fusions, over-expression, truncation, and nucleic acid variation of ret and depdc1 in cancers..
Insight Genetics, Inc


Method and use in temperature controlled processing of microfluidic samples


Embodiments of the invention comprise microfluidic devices, instrumentation interfacing with those devices, processes for fabricating that device, and methods of employing that device to perform pcr amplification. Embodiments of the invention are also compatible with quantitative polymerase chain reaction (“qpcr”) processes.
Caliper Life Sciences, Inc.


Real-time pcr assay for detection of babesia microti and clinical utilization in diagnosis and treatment of babesiosis


Methods and kits are provided for detecting babesia microti using real-time polymerase chain reaction.. .
Westchester Medical Center Advanced Physician Services, Pc


Sample processing apparatus and automatic analyzing apparatus including the same


A sample processing apparatus is for extracting a nucleic acid from a sample and amplifying the nucleic acid, wherein the sample processing apparatus including a housing including a chamber; a valve positioned below the housing; and a polymerase chain reaction (pcr) portion positioned below the valve, wherein the polymerase chain reaction portion performing a polymerase chain reaction.. .
Infopia Co., Ltd.


Method for multiplexed nucleic acid patch polymerase chain reaction


The invention encompasses a method for amplifying at least two different nucleic acid sequences. In particular, the method encompasses a multiplexed nucleic acid patch polymerase chain reaction..
Washington University


Electrokinetic polymerase chain reaction (pcr) devices and methods


Described herein are microfluidic diagnostic methods and devices using electrokinetic modules for the isolation of targets (e.g., cells, bacteria, biomolecules) from biological samples, pcr amplification of dna isolated from the targets, and real-time quantification of the amplified dna using impedance sensing. Sample preparation, pcr amplification, and impedance sensing are thus performed using a single integrated platform..
Genefluidics, Inc.


Methods, devices and systems for emulsion/droplet pcr


The present invention relates generally to the use of a class of surfactants for emulsion and droplet polymerase chain reaction (“pcr”) mixtures. The class of surfactants consists of those having the chemical formula r—(och2ch2)n—oh, wherein r is an alkyl group consisting of 12 to 18 carbons and n is 2 to 25.
Canon U.s. Life Sciences, Inc.


Novel compounds for use in pcr systems and applications thereof


This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (pcr). Methods for preparing the novel compounds are also described..
Life Technologies Corporation


Clamp for fast pcr heating


This disclosure provides a thermocycler system for performing polymerase chain reaction. The thermocycler system can comprise a plurality of bus bars, a microplate, a clamp and a current application device in electrical communication with the bus bars.
Bjs Ip Limited


Removal of pcr inhibitors


Provided herein is technology relating to processing and preparing samples and particularly, but not exclusively, to methods, systems, and kits for removing assay inhibitors, e.g., compounds that inhibit polymerase chain reaction, from samples comprising nucleic acids. In particular, the technology is directed toward treating crude sample preparations, such as supernatants from homogenized stool samples, with insoluble polyvinylpyrrolidone (pvp) to form pvp-assay inhibitor complexes, and filtration to separate the pvp-assay inhibitor complexes from the crude sample preparations to produce clarified samples that exhibit reduced assay inhibition..
Exact Sciences Corporation


Method for screening reagents used in pcr assays


The present invention relates to methods for screening of reagents used in the performance of polymerase chain reaction (pcr) assays. The invention has applications for genotyping, pathogen detection and in vitro diagnostics..
Roche Molecular Systems, Inc.


System for pcr sample preparation and for pcr set-up


A method of setting up a polymerase chain reaction (“pcr”) includes providing a loading device configured to receive sample tubes for setting up a pcr; providing a tube holding device comprising at least a first receiving opening; attaching the tube holding device to the loading device; and aligning at least one receiving opening of the tube holding device with one of the first and second receiving openings of the loading device and simultaneously covering the other of the first and the second receiving openings of the loading device.. .
Vela Operations Pte. Ltd.


Multiplexed analyses of test samples


The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule.
Somalogic, Inc.


Methods and compositions for universal detection of nucleic acids


Provided are methods and compositions for detecting the presence or amount of one or more target nucleic acids in a sample. Methods of the present invention include linking universal nucleic acid segments into a single molecule in a linking reaction dependent on a target nucleic acid of interest.
Unitaq Bio


Ultrasensitive detection and characterization of clustered kras mutations using peptide nucleic acid clamp pcr in drop-based microfluidics


This disclosure employs the combination of a microfluidics platform and drop-based digital polymerase chain reaction (dpcr) to create a breakthrough technology that enables the detection of ctc genes and the isolation of single ctcs from the blood. In the first method, cdna molecules from lysed ctcs are amplified in microfluidic drops and detected via fluorescence signal.

Technique combining pcr and loop-mediated isothermal amplification for the detection of nucleic acids


The present invention relates to a method and a kit of parts for detecting the presence or absence of one or more target nucleic acid sequences in a sample, the method comprising a sequence of steps for pre-amplifying the sample by means of a polymerase chain reaction, followed by a sequence of steps comprising an isothermal amplification of the pre-amplified sample, wherein the isothermal amplification comprises a pair of primers comprising a forward primer having a 3′ part that is substantially complementary to a first part of the target sequence, the presence or absence of which is to be detected, and a 5′ part that is substantially homolog to a second part of the target sequence, and a reverse primer comprising a 3′ part that is substantially homolog to a fourth part of the target sequence and a 5′ part that is substantially complementary to a third part of the target sequence.. .

Method for evaluation of viability of viruses with lymphotropism properties


Methods and techniques to increase the reliability of detecting virus infections, particularly lymphotropism, to eliminate false negative reactions in testing blood for the presence of lymphotropic viruses during enzyme immunoassay (eia) and polymerase chain reaction (pcr) testing, and to better detect viruses with lymphotropism in biological materials having a concentration of virus particles lower than the sensitivity threshold of existing eia and pcr methods, thereby making the techniques of the present invention more reliable.. .
Obschestvo S Ogranichennoi Otvetstvennostyu


Nucleic acid-free thermostable enzymes and methods of production thereof


The present invention provides thermostable enzymes, such as dna polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (pcr)..
Life Technologies Corporation


Microdroplet-manipulation automated execution of molecular biological protocols


Disclosed herein are automated systems for performing various biochemical and molecular biological procedures, including processor-controlled execution of protocols involving multiple steps performed in, on, or with liquid microdroplets. Example protocols are the various polymerase chain reaction (pcr) protocols, but the subject systems are not limited to performing pcr protocols.
The Arizona Board Of Regents On Behalf Of The University Of Arizona


Multiplex probes


Methods and reagents suitable for conducing polymerase chain reaction are described. In particular, the disclosure provides probes and primers that are suitable in dynamic flux amplification procedures.
Fluoresentric, Inc


Sequence, technique platform, and in vitro detecting clostridium difficile ribotype 027


The invention relates to a sequence, a technique platform, and a method for in vitro detecting clostridium difficile ribotype 027. The technology platform includes a pair of primers specific to c.
National Cheng Kung University




Polymerase Chain Reaction topics:
  • Polymerase Chain Reaction
  • Polymerase
  • Nucleic Acid
  • Nucleotide
  • Quantitative
  • Amplification
  • Chlamydia Trachomatis
  • Nucleic Acids
  • Transcription
  • Oligonucleotide
  • Sequencing
  • Real Time Pcr
  • Differentiation
  • Lymphogranuloma Venereum
  • Recombinant


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