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Polymerase Chain Reaction patents



      
           
This page is updated frequently with new Polymerase Chain Reaction-related patent applications. Subscribe to the Polymerase Chain Reaction RSS feed to automatically get the update: related Polymerase RSS feeds. RSS updates for this page: Polymerase Chain Reaction RSS RSS


Date/App# patent app List of recent Polymerase Chain Reaction-related patents
02/26/15
20150056171
 Methods of predicting clinical outcome of chronic lymphocytic leukemia patent thumbnailnew patent Methods of predicting clinical outcome of chronic lymphocytic leukemia
Provided are methods of predicting the outcome of a chronic lymphocytic leukemia (cll) in a subject. The methods comprise performing a polymerase chain reaction assay for an igh locus on a nucleic acid from a biological sample from a subject with cll, wherein the pcr assay amplifies a non-coding region of the igh locus, sequencing a product from the pcr assay, and determining a level of mutation in the non-coding region of the igh locus.
University Of Rochester
02/19/15
20150050660
 Stable compositions for nucleic acid amplification and sequencing patent thumbnailStable compositions for nucleic acid amplification and sequencing
The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable dna polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing.
Life Technologies Corporation
02/12/15
20150044727
 Adaptive thermal block temperature control method and system patent thumbnailAdaptive thermal block temperature control method and system
Aspects of the present teachings describe a method and apparatus for automatically controlling a block temperature to reduce undershooting and overshooting of the temperatures of a sample contained in the block and participating in a polymerase chain reaction (pcr). The adaptive thermal block temperature control begins when a sample temperature enters a sample window region between a preliminary setpoint temperature and a target setpoint temperature for the sample.
Applied Biosystems, Llc
02/12/15
20150044683
 Composition for hot-start reverse transcription reaction or hot-start reverse transcription polymerase chain reaction patent thumbnailComposition for hot-start reverse transcription reaction or hot-start reverse transcription polymerase chain reaction
A composition for hot-start reverse transcription reaction and a composition for reverse transcription pcr are disclosed. The composition is obtained by adding pyrophosphate and pyrophosphatase to an aqueous solution containing reaction buffer solution, mgcl2, four kinds of dntps, and reverse transcription polymerase in a single reaction tube.
Bioneer Corporation
02/05/15
20150038341
 Digital amplification patent thumbnailDigital amplification
The identification of pre-defined mutations expected to be present in a minor fraction of a cell population is important for a variety of basic research and clinical applications. The exponential, analog nature of the polymerase chain reaction is transformed into a linear, digital signal suitable for this purpose.
The Johns Hopkins University
02/05/15
20150038336
 Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions patent thumbnailMethod for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions
The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction.
Cornell University
02/05/15
20150037795
 Method and primer set for detecting mutation patent thumbnailMethod and primer set for detecting mutation
Disclosed herein is a polymerase chain reaction (pcr)-based method for detecting an insertion/deletion variant, as compared with a reference sequence, in a selected region of a target gene in a sample. The target gene has a template strand and a coding strand complementary to the template strand.
Mackay Memorial Hospital
01/29/15
20150031029
 Method of detecting residual genomic dna and a kit thereof patent thumbnailMethod of detecting residual genomic dna and a kit thereof
The present disclosure relates to a highly specific and sensitive method of detecting host cell impurities in a biological sample by using quantitative real time polymerase chain reaction (q pcr). The present disclosure also provides novel designed primer and probe to amplify only the specific alu family of dispersed repetitive sequences from chinese hamster ovary cells used for expression of therapeutic proteins..
Biocon Limited
01/22/15
20150024434
 Method for obtaining fab fragments from single antibody producing cells by multiplexed cpr in combination with taqman probes patent thumbnailMethod for obtaining fab fragments from single antibody producing cells by multiplexed cpr in combination with taqman probes
Herein is reported a method for a multiplex one tube real-time reverse-transcriptase gene-specific polymerase chain reaction for the amplification and quantification of cognate igg heavy and light chains encoding nucleic acids (human igg isotype) from a single cell.. .
Hoffmann-la Roche Inc.
01/22/15
20150020532
 Active, micro-well thermal control subsystem patent thumbnailActive, micro-well thermal control subsystem
Devices and systems for active thermal control of sample holding devices for bdna testing, polymerase chain reaction testing, chemiluminescent immuno-assay testing, and so forth. The thermal control subsystem includes a fluidic circuit, first and second heater assemblies, a centrifugal pump, and a heat exchange device.
Siemens Healthcare Diagnostics Inc.
01/15/15
20150018249

Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions


The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (pcr) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence.
Cornell Research Foundation, Inc.
01/15/15
20150017688

Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)


The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (rt-pcr). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step rt-pcr procedure using combinations of reverse transcriptase and thermostable dna polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin.
Life Technologies Corporation
01/15/15
20150017654

Absolute pcr quantification


The present application provides methods and devices for absolute quantification of polymerase chain reaction target nucleic acids. In particular, the methods and devices of the present application provide for splitting a nucleic acid sample to be analyzed into small, isolated volumes, conducting the method of polymerase chain reaction (pcr) on said volumes, detecting pcr amplification products, analyzing said detected pcr amplification products, performing absolute quantification of the pcr target and presenting said quantification results..
The Research Foundation For The State University Of New York
01/15/15
20150017653

Absolute pcr quantification


The present application provides methods and devices for absolute quantification of polymerase chain reaction target nucleic acids. In particular, the methods and devices of the present application provide for splitting a nucleic acid sample to be analyzed into small, isolated volumes, conducting the method of polymerase chain reaction (pcr) on said volumes, detecting pcr amplification products, analyzing said detected pcr amplification products, performing absolute quantification of the pcr target and presenting said quantification results..
The Research Foundation For The State University Of New York
01/15/15
20150017645

Qualitative and quantitative detection of microbial nucleic acids


The present invention relates to new methods and uses for the qualitative and quantitative detection of microbial nucleic acids using at least a first control nucleic acid, or a first and a second control nucleic acid in different concentrations. The method is based on amplification of nucleic acids, for example the polymerase chain reaction.
Roche Molecular Systems, Inc.
12/25/14
20140377810

Ssb-polymerase fusion proteins


Fusion proteins comprising a single strand dna binding protein and a nucleic acid polymerase (e.g. Dna polymerase or reverse transcriptase).
Life Technologies Corporation
12/25/14
20140377757

Compositions and methods for screening for creatine transporter deficiency


Amplification primers, sequencing primers, kits for screening, and screening methods for identifying a slc6a8 creatine transporter gene mutation are disclosed. The screening method includes treating a sample of dna with polymerase chain reaction amplification primers for amplifying regions of the dna having slc6a8 to produce a first, second, and third amplification product, sequencing the first, second, and third amplification products with sequencing primer pairs to provide a dna sequence of slc6a8 in the sample, and comparing the dna sequence of slc6a8 with a reference dna sequence of slc6a8..
University Of Cincinnati
12/11/14
20140360879

Control of chemical reactions using isotachophoresis


Isotachophoresis (itp) is exploited to control various aspects of chemical reactions. In a first aspect, at least one of the reactants of a chemical reaction is confined to an itp zone, but the resulting product of the chemical reaction is separated from this itp zone by the itp process.
11/27/14
20140349293

Method and kit for protozoa characterization


Disclosed are compositions, kits, and methods for detecting, extracting, visualizing, characterizing, and/or identifying one or more protozoa. Various types of polymerase chain reaction techniques in connection with specifically designed oligonucleotide probes can be used to detect a variety of, e.g., pathogenic, protozoa in samples..
11/20/14
20140342366

Modification of sample preparation to differentiate live and dead bacteria by polymerase chain reaction assay


The invention relates to a method of determining whether a live microbe, such as bacteria, is present in a test sample.. .
11/13/14
20140335525

Cutoff point delta ct. her2 pcr testing in breast cancer


The present invention is related to an improved method for her2 gene test by using quantitative real-time pcr (polymerase chain reaction) technique. Our invention streamlines test process, and incorporates quality control for each major step, including sample, reagent, operation, and data report.
11/06/14
20140328733

Simplified gating sealing and flow control in micro and nano devices


A biochip for multiplex genetic identification is disclosed. An biochip for separating and detecting a plurality of dna fragments includes a set of inputs and chambers for receiving a sample matrix of genetic material and reagents needed to conduct a polymerase chain reaction amplification of the genetic material.
10/23/14
20140315757

Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions


The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (pcr) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence.
10/23/14
20140315202

Method for assembling multiple target loci to single nucleic acid sequence


A method for assembling multiple target loci to a single assembled nucleic acid sequence involves assembling the multiple target loci to a single assembled nucleic acid sequence through two polymerase chain reactions (pcrs). A pair of primers for a primary amplification include a target-specific sequence and a 5′-flanking assembly spacer sequence.
10/23/14
20140315201

Method for high-throughput screening of transgenic plants


The invention relates to a method for quantifying levels of expression and/or quantifying copy number of a heterologous polynucleotide in a transgenic plant using quantitative or real-time polymerase chain reaction (qpcr or real-time pcr), wherein the real-time pcr is performed using a primer set specific to a heterologous terminator sequence operably linked to the heterologous polynucleotide.. .
10/16/14
20140308708

Error correction in nucleic acid molecules


An enzymatic method for removing sequence errors in nucleic acid molecules are described. The method utilizes a cel endonuclease that cuts heteroduplexes at mismatch sites containing the errors and an overlap extension polymerase chain reaction to re-assemble the cleaved fragments into full-length nucleic acid molecules free of the errors..
10/16/14
20140308666

Development of novel detergents for use in pcr systems


This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (pcr). Methods for preparing the modified detergents are also described..
10/09/14
20140302562

Fast pcr heating


Provided herein is a microplate for polymerase chain reaction (pcr), comprising a substrate formed of a material that is susceptible to heating pcr samples upon the application of an electromagnetic field and/or electromagnetic energy to said substrate. The substrate provides a pcr ramp rate of at least 5° c./second upon the application of an electromagnetic field and/or electromagnetic energy to said substrate..
10/09/14
20140302503

Compositions, methods and systems for polymerase chain reaction assays


The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddpcr) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddpcr assays using intercalating dyes.
10/02/14
20140295441

Cartridge interface module


A cartridge interface module (cim), configured to engage with a removable microfluidic cartridge in a nucleic acid analyzer system can include a fluidics component, which is configured to initiate and support a liquid extraction of nucleic acids from a biological sample contained in the removable microfluidic cartridge. The cim also includes a polymerase chain reaction (pcr) assembly component which can be configured to initiate and support amplification of the extracted nucleic acids.
10/02/14
20140295434

Detection micro-rna with high specificity


The invention provides a method for detecting mirna based on polymerase chain reaction comprising evagreen dye, and the use of evagreen dye in elevating the binding specificity of primers to templates in pcr.. .
09/25/14
20140288289

Method for detecting and quantifying wheat endogenous gene


Provided is a method of detecting or quantifying a wheat species-specific dna in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having at partial sequence of a nucleotide sequence identified as seq id no: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule..
09/25/14
20140287420

Microfluidics polymerase chain reaction and high resolution melt detection


The present invention relates to a method and system for polymerase chain reaction (“pcr”), high resolution melt (“hrm”) analysis and microfluidics, and, more specifically, to a method and system for implementing the processes of pcr and hrm on a microscale in a microfluidics chamber for certain purposes including for purposes of dna detection and/or extraction.. .
09/25/14
20140287405

Method for detecting and quantifying wheat endogenous gene


Provided is a method of detecting or quantifying a wheat species-specific dna in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having a partial sequence of a nucleotide sequence identified as seq id no: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule..
09/18/14
20140273185

Tactical and portable pcr/hrm genome identification system


The present invention relates to a polymerase chain reaction and high resolution melt genetic identification system, and, more specifically, to a tactical and portable polymerase chain reaction and high resolution melt genetic analysis and identification system that is configured to determine and communicate analysis and identification results and a tiered confidence/alert level related to the analysis and identification results.. .
09/18/14
20140273099

Methods and devices for non-therman polymerase chain reaction


Methods and devices for non-thermal pcr amplification of nucleic acid sequences. An electrical potential is applied to cause non-thermal separation of strands of a double-stranded nucleic acid or double-stranded nucleic acid/primer extension product..
09/18/14
20140272998

Diagnostic microrna profiling in cutaneous t-cell lymphoma (ctcl)


The present invention relates to the field of cancer-diagnostics. In particular the invention relates to a microrna expression signature that allows discriminating skin samples of cutaneous t-cell lymphomas (ctcl) from non-malignant (inflammantory) skin samples by use of quantitative polymerase chain reaction performed on reverse transcribed mirna.
09/18/14
20140272984

Microfluidic flow monitoring


The present invention relates to systems and methods of monitoring velocity or flow in channels, especially in microfluidic channels. In some embodiments, the present invention relates to systems and methods of monitoring velocity or flow rate in systems and methods for performing a real-time polymerase chain reaction (pcr) in a continuous-flow microfluidic system..
09/18/14
20140272977

Molecular targets and methods for formulation screening and preservative efficacy testing


Methods and kits are disclosed for distinguishing viable from nonviable microbial cells. The methods and kits are useful in the screening of cell culture formulations and the testing of preservative efficacy.
09/18/14
20140272927

Methods, devices, and systems for processing multiple assays background


Methods, devices, and systems for performing polymerase chain reaction (pcr) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both pcr temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the pcr temperature cycling for real-time pcr and/or during temperature ramping in the melt data acquisition.
09/04/14
20140249142

Discovery of a somatic mutation in myd88 gene in lymphoplasmacytic lymphoma


Diagnostic assays for facilitating the diagnosis of lymphoplasmacytic lymphoma (lpl) are provided. The method comprises assessing a biological sample of the subject for the presence of a mutation at position 38182641 in chromosome 3p22.2, wherein presence of the mutation is indicative that the subject has lpl.
09/04/14
20140248711

Apparatus and electrical detection of oligonucleotides through pore blockades


Systems and methods for specific nucleic acid (na) sequence detection that do not rely on polymerase chain reaction (pcr) for target sequence amplification and do not require any special reagents other than a complementary sequence capture probe conjugated to spherical beads.. .
09/04/14
20140248622

Telomere length measurement in formalin-fixed, paraffin embedded (ffpe) samples by quantitative pcr


Methods of reliably quantifying telomere length in cells or tissues that have been formalin fixed and paraffin embedded (ffpe) samples by quantitative polymerase chain reaction protocol and kits for use with such various methods are provided. The methods of the present invention may be used to predetermine an individual's response to treatment with a telomerase inhibitor, a telomere damaging agent or a telomerase activator..
09/04/14
20140248613

Automatic detection kit for detecting hla alleles using real-time polymerase chain reaction


Provided is an automatic detection kit for automatically detecting hla alleles using a real-time polymerase chain reaction (pcr). The real-time pcr is performed on dna isolated from a sample using a primer which is able to specifically bind to hla alleles and a fluorescent probe which is able to detect amplification of the hla alleles in real time, and the hla allele typing is performed by analyzing a fluorescence value obtained from the real-time pcr using an hla automatic typing..
08/28/14
20140243242

Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence


The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a single reaction method to co-amplify multiple subsequences of a nucleic acid fragment sequence (i.e., for example, at least two read pairs from a single library insert sequence).
08/28/14
20140242594

Methods and compositions for preparation of nucleic acids


A method for isolating genomic dna (gdna) from a biological material. In some embodiments, the method includes (a) contacting a sample that contains gdna with a solution of hydroxide and a detergent under conditions and for a time sufficient to degrade a cell wall, a cell membrane, a nuclear membrane, a nucleoprotein, or combinations thereof and/or to denature the gdna; (b) mixing into the solution resulting from step (a) a solution characterized by high salt and sufficient buffering capacity to reduce the ph of the solution to less than 10, thereby producing a neutralized solution; (c) centrifuging the sample at a speed and length of time sufficient to clarify the preparation; and (d) removing insoluble material from the neutralized and clarified preparation, whereby a solution of gdna is produced.
08/21/14
20140236496

Methods and systems for visualizing and evaluating data


A computer-implemented method of generating a digital polymerase chain reaction (dpcr) result is provided. The method includes detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period.
08/21/14
20140235464

Detection and quantification of biomolecules using mass spectrometry


The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry.
08/21/14
20140234850

Dna fragment detection method, dna fragment detection kit and the use thereof


The disclosure claims a cleaved deoxyribonucleic acid (dna) detection method, a dna fragment detection kit and use thereof. Wherein, the method includes the steps of: designing primers according to a test site or a test region of the dna fragment; cyclizing the dna fragment to obtain acyclized dna; implementing polymerase chain reaction (pcr) amplification for the cyclized dna by using the primers; and detecting the pcr amplification product.
08/14/14
20140228256

Method and kit for constructing plasma dna sequencing library


The disclosure relates a method and a kit for constructing a plasma deoxyribonucleic acid (dna) sequencing library. The method provided by the disclosure includes: extracting a plasma dna; making the plasma dna ligate to a sequencing linker, and purifying a ligation product; performing polymerase chain reaction (pcr) amplification for the purified ligation product, purifying the pcr amplification product, and obtaining the plasma dna sequencing library, wherein, the method does not include the step of performing 5′-terminus phosphorylation for the plasma dna.
08/14/14
20140228223

High throughput paired-end sequencing of large-insert clone libraries


The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a paired end sequencing method that improves the yield of long-distance genomic read pairs by constructing long-insert clone libraries (i.e., for example, a fosill library or a foscn library) and converting the long-insert clone library using inverse polymerase chain reaction amplification or shearing and recircularization of shortened fragments into a library of co-ligated clone-insert ends.
07/31/14
20140213485

Methods for preparing cdna from low quantities of cells


Methods for preparing cdna libraries from single and low quantities of cells are disclosed. The methods are based on the principles of multi-strand displacement amplification or semi-random primed polymerase chain reaction.
07/31/14
20140212884

Composition and methods for rt-pcr comprising an anionic polymer


The present invention is in the fields of molecular biology. The present invention is directed to novel compositions, methods and kits useful for the generation of nucleic acids from an rna template and further nucleic acid replication.
07/24/14
20140206855

Methods and compositions for detecting cancers associated with methylation of hmlh1 promoter dna


Methods are provided for detection of cancers associated with methylation of hmlh1 promoter dna in a subject. The method comprise assaying for the presence of methylated hmlh1 promoter dna in a bodily fluid from a subject.
07/17/14
20140200167

Functionally integrated device for multiplex genetic identification


A biochip for multiplex genetic identification is disclosed. An biochip for separating and detecting a plurality of dna fragments includes a set of inputs and chambers for receiving a sample matrix of genetic material and reagents needed to conduct a polymerase chain reaction amplification of the genetic material.
07/17/14
20140199731

Assay and other reactions involving droplets


The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel.
07/17/14
20140199730

Assays and other reactions involving droplets


The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel.
07/03/14
20140186828

Methods for determining cell viability using molecular nucleic acid-based techniques


The present invention relates to novel methods, and kits, for selectively excluding dead cells from a mixture containing live and dead cells, such as microbe cells in clinical samples, blood products, medical/biotechnology products and food products where subsequent interrogation of the selected live cells are an indicator of the presence of microbe viability. In particular, the invention relates to improved methods for performing direct nucleic acid amplification techniques such as polymerase chain reaction (pcr) and isothermal techniques in blood and other body fluids, for correlation with microbe cell viability from bacteremia and fungemia samples.
06/26/14
20140178880

Method of preparing a reaction mixture and related products


The invention relates to a method of preparing a reaction mixture for polymerase chain reaction (pcr) assay and a solution set for pcr. The method comprises providing a sample solution comprising a biological sample to be amplified in said pcr assay and first colorant providing the solution a first color, providing a reagent solution comprising at least one other substance required for performing said assay and second colorant providing the solution a second color different from the first color, and mixing the sample solution and the first reagent solution for providing a mixed solution to be subjected to the pcr process, the mixed solution having, due to said first and second colorants, a third color different from the first and second colors.
06/19/14
20140171334

Gene analysis method using sdl-pcr


According to the sdl-pcr method of the present invention, non-specific amplification can be minimized by removing non-ligated probes or genomic dna using a tag, and separation can be achieved within a shorter time compared to a separation method that is performed using exonuclease. In addition, ligation, separation and polymerase chain reaction processes can be performed in a single solution in a single tube, and thus a plurality of genes can be amplified at the same time in an accurate and rapid manner..
06/19/14
20140170706

Low-mass sample block with rapid response to temperature change


A sample block for use in the polymerase chain reaction, dna sequencing, and other procedures that involve the performance of simultaneous reactions in multiple samples with temperature control by heating or cooling elements contacting the bottom surface of the block is improved by the inclusion of hollows in the block that are positioned to decrease the mass of the block in the immediate vicinity of the wells.. .
06/12/14
20140162321

Preparation of gene-specific templates for the use in single primer amplification


This disclosure relates to methods for creating engineered templates that are useful for amplification of one or more antibody genes without the use of gene-specific primers. More specifically, templates engineered using these methods in a polymerase chain reaction setting which allows for the specific amplification of one or more antibody genes..
06/12/14
20140162267

Dry composition of reaction compounds with stabilized polymerase


The present invention provides methods to obtain dry compositions of reaction compounds that maintain the biological activity of the compounds upon re-solubilization after a certain storage time. Preferably, the dry composition comprises a polymerase, and the dry composition is usable for polymerase chain reaction (pcr) amplification after re-solubilization..
06/12/14
20140162266

Methods for polymerase chain reaction copy number variation assays


This disclosure provides methods for measuring the copy number for highly amplified and/or abundant genomic loci. Recognized herein is a need for methods for determining nucleic acid copy number, particularly in instances where one locus to be quantified (i.e., the target) is relatively more abundant than a locus of known abundance (i.e., the reference).
06/12/14
20140162261

Detection kit for identifying genotype in depression patients and using the same


The present invention relates to a rs6311 test kit, which includes a probe, a primer, and a polymerase chain reaction solution, wherein said probe sequence is as follows: rs6311t-fam: ctgtgagtgtctggc (seq. Id.
06/05/14
20140154681

Methods to predict breast cancer outcome


The present invention provides methods for predicting the outcome of a subject having breast cancer. The prediction model is based on the gene expression profile of the genes listed in table 1a or 1b.
05/29/14
20140147849

Quantitation of human genomic and mitochondrial dna


Methods are provided for determining, in a single polymerase chain reaction (pcr) reaction, the quantity, quality, and gender of origin of dna in a sample, and whether or not the sample contains pcr amplification inhibitors. The methods involve carrying out a single pcr multiplex reaction utilizing primer sets specific for amplifying: the human amelogenin locus; an x- and/or y-chromosome specific gene that is shorter than the amelogenin gene; at least one mitochondrial dna sequence, and preferably two differently-sized mitochondrial sequences; and a heterologous, non-human reporter gene..
05/22/14
20140141419

Single tube quantitative polymerase chain reaction (pcr)


Provided herein are systems and methods for quantitatively monitoring target amplicons produced by polymerase chain reaction (pcr). In particular, quantitative monitoring of target amplicon(s) in a single-tube pcr reaction without separate calibration reactions are provided..


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Polymerase Chain Reaction topics: Polymerase Chain Reaction, Polymerase, Nucleic Acid, Nucleotide, Quantitative, Amplification, Chlamydia Trachomatis, Nucleic Acids, Transcription, Oligonucleotide, Sequencing, Real Time Pcr, Differentiation, Lymphogranuloma Venereum, Recombinant

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