 Patent Application Title |
Patent App Num. |
Date |
 Production and use of bacterial histamine | 20130149291 | 20130613 |
 A method is provided of selecting specific probiotic lactic acid bacteria producing histamine and the use of such strains for beneficial effects for mammals. The method includes selecting a lactic acid bacterial strain for use in the local production of histamine in a mammal, and further comprises screening bacteria for the presence of an. active histidine operon and selecting a strain which has an active histidine operon and is capable of producing histamine. Preferably said strain is selected for its ability to produce histamine at a level of greater than 250 pg/ml. The present invention further provides products comprising the strains obtainable by the selection methods of the invention for use in the local production, of histamine in a mammal, in particular for use in the... |
| Bacteriophages for use against bacterial infections | 20130121967 | 20130516 |
 The present invention relates to a composition comprising obligate lytic bacteriophages generated by a method comprising subjecting normally in vivo lysogenic, pseudolysogenic or temperate bacteriophages to genetic modifications in vitro, which alters the biological activity of one or more of the individual gene products for establishing, maintaining, controlling or regulating the lysogenic life cycle of the bacteriophages, thereby converting them to obligate lytic bacteriophages, wherein the genetic modification includes modification of a single gene in the operon containing a gene resulting in a gene product for establishing, maintaining, controlling or regulating the lysogenic life cycle of the bacteriophages.
... |
| Genes encoding key catalyzing mechanisms for ethanol production from syngas fermentation | 20130102044 | 20130425 |
 Gene sequences of key acetogenic clostridial species were sequenced and isolated. Genes of interest were identified, and functionality was established. Key genes of interest for metabolic catalyzing activity in clostridial species include a three-gene operon coding for CODH activity, a two-gene operon coding for PTA-ACK, and a novel acetyl coenzyme A reductase. The promoter regions of the two operons and the acetyl coA reductase are manipulated to increase ethanol production.
... |
| Pantothenic acid biosynthesis in zymomonas | 20130078694 | 20130328 |
 Zymomonas is unable to synthesize pantothenic acid and requires this essential vitamin in growth medium. Zymomonas strains transformed with an operon for expression of 2-dehydropantoate reductase and aspartate 1-decarboxylase were able to grow in medium lacking pantothenic acid. These strains may be used for ethanol production without pantothenic acid supplementation in seed culture and fermentation media.
... |
| Autoluminescent plants including the bacterial lux operon and methods of making same | 20130074221 | 20130321 |
 In one aspect, the invention relates to a transgenic autoluminescent plant including an expressible heterologous nucleotide sequence comprising a bacterial LUX operon, which includes LUX A. LUX B. LUX C. LUX D. LUX b. And LUX G genes, wherein the heterologous nucleotide sequence is expressed to render the plant autonomously luminescent.
... |
Subscribe to updates on this page: Operon RSS  |
| Production and use of bacterial histamine | 20130022586 | 20130124 |
| A method is provided of selecting specific probiotic lactic acid bacteria producing histamine and the use of such strains for beneficial effects for mammals. The method includes selecting a lactic acid bacterial strain for use in the local production of histamine in a mammal, and further comprises screening bacteria for the presence of an active histidine operon and selecting a strain which has an active histidine operon and is capable of producing histamine. Preferably said strain is selected for its ability to produce histamine at a level of greater than 250 pg/ml. The present invention further provides products comprising the strains obtainable by the selection methods of the invention for use in the local production of histamine in a mammal, in particular for use in the... |
| Streptococcus thermophilus bacterium | 20130004616 | 20130103 |
| The present invention provides an improved Streptococcus thermophilus. The invention is based on the surprising finding that a putative lantibiotic operon in Streptococcus thermophilus is required for community formation. The operon is a 6.4 kb region that comprises three open reading frames: a lantibiotic biosynthesis protein (dehydratase—SWISSPROT REF: Q5M6E4), a lantiobiotic biosynthesis protein (cyclase—SWISSPROT REF: Q5M6E3) and a lantibiotic efflux protein (permease—SWISSPROT REF: Q70C59). Inhibiting the function of this operon inhibits community formation. Streptococcus thermophilus with a reduced ability to form communities are useful in the processing, producing or manufacturing of dairy products, in particular cheese, where Streptococcus thermophilus are often used as a starter culture.
... |
| Synthetic operon | 20120225454 | 20120906 |
| The present invention relates to synthetic operons. In particular, the present invention relates to a synthetic operon for integration into a bacterial chromosome of a bacterium comprising a promoter operably-linked to at least two genes, wherein at least one gene is a gene of interest and at least one gene is a gene essential to said bacterium.
... |
| Vector comprising mannose promoter and mannose promoter | 20120202246 | 20120809 |
| The present invention relates to a vector expressible in a prokaryotic host and a nucleic acid sequence comprising a mannose-inducible promoter of the mannose operon of Bacillus subfiles wherein the vector and nucleic acid sequence, respectively, can be suitably used for transforming a host cell for expression of a heterologous nucleic acid sequence coding a polypeptide in, in particular, high cell density fermentation.
... |
| Fermentation process | 20120196323 | 20120802 |
| The present invention relates to a fermentation process for culturing a host cell comprising a vector which comprises an inducible promoter of the mannose operon wherein the inducible promoter of the mannose operon controls expression of a nucleic acid sequence encoding for a polypeptide, in particular the present invention relates to the high cell density fermentation of a prokaryotic host cell comprising the inducible promoter of the mannose operon in the production of a polypeptide.
... |
| Methods for producing hyaluronan in a recombinant host cell | 20120149067 | 20120614 |
| The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding... |
| Rhamnose promoter expression system | 20120135463 | 20120531 |
| b) a prokaryotic signal sequence operably linked to the nucleic acid sequence. The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.
... |
| Salmonella enterica presenting c. jejuni n-glycan or derivatives thereof | 20120100177 | 20120426 |
| The present invention relates to Salmonella enterica comprising at least one pgl operon of Campylobacter jejuni or a functional derivative thereof and presenting at least one N-glycan of Campylobacter jejuni or N-glycan derivative thereof on its cell surface. In addition, it is directed to medical uses and pharmaceutical compositions thereof as well as methods for treating and/or preventing Campylobacter and optionally Salmonella infections and methods for producing these Salmonella strains.
... |
Subscribe to updates on this page: Operon RSS |
| Melibiose operon expression system | 20120077225 | 20120329 |
| New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic... |
| Secretion expression of antibiotic peptide cad in bacillus subtilis and expression system of recombination bacillus subtilis | 20120009625 | 20120112 |
| The present invention relates to a method for expressing antimicrobial peptide CAD by means of a recombinant Bacillus subtilis expression system. The SUMO protease expression operon is first artificially synthesized. The protein expression operon genes of Saccharomyces cerevisiae small ubiquitin-related protein is then fused with the antibacterial peptide AD. The fusion protein is further cloned into the pNF11 plasmid to be introduced into Bacillus subtilis, thereby ensuring the induced expression of recombined Bacillus subtilis in shake flasks. The method has the advantages of a simple expression system, large-scale production, low production cost, strong biological activity and no toxic or harmful substance production. Moreover, the method provides a medicine with low price and strong antibacterial capacity for clinic disease prevention and treatment. This invention can also be... |
| Methods for producing hyaluronan in a recombinant host cell | 20110014662 | 20110120 |
| The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding... |
| Genes encoding key catalyzing mechanisms for ethanol production from syngas fermentation | 20110008860 | 20110113 |
| Gene sequences of key acetogenic clostridial species were sequenced and isolated. Genes of interest were identified, and functionality was established. Key genes of interest for metabolic catalyzing activity in clostridial species include a three-gene operon coding for CODH activity, a two-gene operon coding for PTA-ACK, and a novel acetyl coenzyme A reductase. The promoter regions of the two operons and the acetyl coA reductase are manipulated to increase ethanol production.
... |
| Production and secretion of glucose in photosynthetic prokaryotes (cyanobacteria) | 20110003345 | 20110106 |
| The present invention includes compositions and methods for making and using an isolated cyanobacterium that includes a portion of an exogenous bacterial cellulose operon sufficient to express bacterial cellulose, whereby the cyanobacterium produces extracellular glucose. The compositions and methods of the present invention may be used as a new global crop for the manufacture of cellulose, CO2 fixation, for the production of alternative sources of conventional cellulose as well as a biofuel and precursors thereof.
... |
| Treatment of protein folding disorders | 20100317690 | 20101216 |
| Described are various compounds and methods for the treatment of disorders arising from aberrant protein folding, including in particular lysosomal storage diseases. In particular, polyhydroxylated alkaloids and imino sugars which are pharmacoperones of an enzyme and which do not bind to a catalytic site of said enzyme are described.
... |
| Promoter and a production method for l-lysine using the same | 20100317067 | 20101216 |
| Disclosed are a nucleic acid molecule of Corynebacterium glutamicum origin, having an improved promoter activity, which is operably linked to operon encoding aspartate kinase and aspartate semialdehyde dehydrogense, a vector containing the same, a transformant transformed with the vector, and a method for the production of L-lysine using the transformant.
... |
| Method for the recombinant production of magnetic nanoparticles | 20100292495 | 20101118 |
| The present invention relates to methods and compositions for the size-adjusted recombinant production of magnetic nanoparticles. More particular, the invention relates to a method, comprising: providing one or more cells being capable of producing magnetic nanoparticles; modifying in the one or more cells the expression of one or more genes involved in the formation of the magnetic nanoparticles; cultivating the modified cells obtained in step (b); and isolating the magnetic nanoparticles from the cultivated cells, wherein the magnetic nanoparticles have a defined size. In preferred embodiments, the method comprises modifying the expression of one or more genes of the mamGFDC operon in magnetotactic bacterial cells. The invention is further directed to host cells bearing said modifications, the recombinant magnetic particles isolated from such cells as well... |
| Transgenic microbial polyhydroxyalkanoate producers | 20100267016 | 20101021 |
| Transgenic microbial strains are provided which contain the genes required for PHA formation integrated on the chromosome. The strains are advantageous in PHA production processes, because (1) no plasmids need to be maintained, generally obviating the required use of antibiotics or other stabilizing pressures, and (2) no plasmid loss occurs, thereby stabilizing the number of gene copies per cell throughout the fermentation process, resulting in homogeneous PHA product formation throughout the production process. Genes are integrated using standard techniques, preferably transposon mutagenesis. In a preferred embodiment wherein multiple genes are incorporated, these are incorporated as an operon. Sequences are used to stabilize mRNA, to induce expression as a function of culture conditions (such as phosphate concentration), temperature, and stress, and to aid in selection, through the... |
| Nontypeable haemophilus influenzae virulence factors | 20100196382 | 20100805 |
| The invention relates to a mutation within the sap operon of an avirulent clone of a nontypeable strain of Haemophilus influenzae (NTHi). The invention also relates to the NTHi sap operon genes and the polypeptides encoded by these polynucleotide sequences. The invention also relates to a novel 110 kDa NTHi outer membrane protein and the polynucleotide that encodes this outer membrane protein. Methods of screening for NTHi infection, and treating and preventing NTHi related disorders are also contemplated.
... |
| Bioluminescent plants comprising bacterial lux operon and methods of making same | 20100192262 | 20100729 |
| In one aspect, the invention relates to a transgenic bioluminescent plant including an expressible heterologous nucleotide sequence comprising a bacterial LUX operon, which includes LUX A, LUX B, LUX C, LUX D, LUX E, and LUX G genes, wherein the heterologous nucleotide sequence is expressed to render the plant bioluminescent.
... |
| Brucella melitensis mutants and methods | 20100158954 | 20100624 |
| Certain attenuated mutants of Brucella, especially B. melitensis, B. abortus, B. suis and B. ovis, when administered to a human or animal trigger a protective immune response such that subsequent challenge with virulent Brucella of the same species does not result in disease or results in much less severe symptoms. Functional inactivation of galE, a virB gene or the operon (ORFs 1087-1090) comprising the gene encoding β-hexosaminidase (BMEI1087) and a lytic murein transglycosylase gene (BMEI1088). A specific example of the attenuated galE mutant which produces a protective immune response is B. melitensis GR024. The specific example of an inactivated ORF1087-1090 operon is B. melitensis GR026; it has an insertion mutation in the promoter region upstream of ORF 1090. Vaccination with live cells of either or both... |
| Hybrid operon for expression of colonization factor (cf) antigens of enterotoxigenic escherichia coli | 20100136059 | 20100603 |
| A recombinant operon comprising a gene assembly wherein there are at least two structural genes coding for at least two major subunits of colonization factor antigens (CFs) associated with enterotoxigenic Escherichia coli bacteria (ETEC), is disclosed. Further disclosed is a host cell, such as an Escherichia coli cell, genetically engineered to comprise such a recombinant operon, wherein said operon is located on an episomal element, such as a plasmid, or integrated in the chromosome of said host cell. Also disclosed is a method of producing a host cell capable of expressing from said operon at least two major subunits of colonization factor antigens (CFs) associated with enterotoxigenic Escherichia coli bacteria (ETEC). In addition, a vaccine composition against diarrhea comprising at least one such host cell together... |
| Inducible/regulated gene expression system in e coli | 20100112636 | 20100506 |
| An expression system for transforming E. coli with a nucleic acid molecule of interest has an operator sequence of a cmt operon operatively linked to a promoter for the operator, and, a repressor sequence from a cym operon operatively linked to a promoter for the repressor. The expression system may have a nucleic acid molecule of interest, for example, a nucleic acid molecule that encodes a protein. Any type of E. coli host cells may be transformed with the expression system. A method of producing a protein involves transforming an E. coli host cell with the expression system having a nucleic acid molecule that codes for a protein, and, culturing the host cell in a culture medium under conditions in which the nucleic acid molecule will... |
| Mutant arabinose promoter for inducible gene expression | 20100068758 | 20100318 |
| An L-arabinose inducible expression system comprising a mutant arabinose promoter. This system exhibits an increase in heterologous protein production upon induction with L-arabinose and comprises a mutant araB promoter and an AraC transcription binding region. This system retains the tight regulatory control characteristic of the wild type arabinose operon.
... |
| Enhanced expression from the pm promoter | 20100048426 | 20100225 |
| The present invention concerns a method of producing a desired gene product in a recombinant gene expression system, said method comprising expressing said gene from a Pm promoter-based expression system using at least two mutant elements selected from: (i) a mutant Pm promoter; (ii) a mutant mRNA leader; and (iii) a mutant XyIS; wherein said mutant elements each comprise one or more mutations which enhance expression of said desired gene. Particularly combinations of a mutant Pm promoter and a mutant mRNA leader are concerned. Isolated nucleic acid molecules, vectors, host cells, libraries, expression systems, methods of enhancing expression, obtaining nucleic acid molecules and identifying combination mutants which enhance expression, artificially constructed operons and their uses are also encompassed.
... |
| Method for constructing an operon containing translationally coupled genes | 20090226919 | 20090910 |
| The present invention provides a method for constructing recombinant translationally coupled operons, a method for producing useful metabolites using the bacterium containing the coupled operons, and a method for monitoring gene expression.
... |
| Production of coenzyme q-10 | 20090226986 | 20090910 |
| The present invention provides improved processes for the preparation of Coenzyme Q-10 (CoQ10) by fermentation of microorganisms of the genus Rhodobacter transformed with the mevalonate operon of Paracoccus zeaxanthinifaciens.
... |
| Method for producing an l-amino acid using a bacterium of the enterobacteriaceae family with enhanced expression of the fucpikur operon | 20090209011 | 20090820 |
| The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to enhance expression of at least one gene of the fucPIKUR operon.
... |
| Method for producing an l-amino acid using a bacterium of the enterobacteriaceae family with enhanced expression of the fucpikur operon | 20090209011 | 20090820 |
| The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to enhance expression of at least one gene of the fucPIKUR operon.
... |
| Ammendola/salmonella enterica strains of reduced pathogenicity, method for their preparation and uses thereof | 20090175913 | 20090709 |
| The invention concerns novel Salmonella enterica attenuated strains, characterized in that they are inactivated at the level of the znuABC operon through mutation in at least one of the znuA, znuB, znuC genes of such operon, for uses in medical or veterinary fields as vaccines.
... |
| Expression system, components thereof and methods of use | 20090176275 | 20090709 |
| Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from Pseudomonas putida. Disclosed herein are novel expression systems and components thereof, which involve the development of a CymR variant with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variant, fusion proteins incorporating such a variant, and its use in the control and expression of polynucleotides. The CymR variant comprises a 142Glu to 142Gly single point mutation of wild type CymR.
... |
| Method for producing an l-amino acid using a bacterium of the enterobacteriaceae family with attenuated expression of the rspab operon | 20090170169 | 20090702 |
| The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the rspAB operon.
... |
| Promoter and plasmid system for genetic engineering | 20090156430 | 20090618 |
| This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.
... |
| Method for producing an l-amino acid using a bacterium of the enterobacteriaceae family | 20090155861 | 20090618 |
| A method is described for producing an L-amino acid, for example L-threonine, L-lysine, L-leucine, L-histidine, L-cysteine, L-phenylalanine, L-arginine, L-tryptophan, L-glutamic acid, L-valine, and L-isoleucine, by fermentation of glucose using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance the activity of the high-affinity arabinose transporter coded by the araFGH operon.
... |
| 1,2 benzoisothiazole derivative, and agricultural or horticultural plant disease- controlling agent | 20090137646 | 20090528 |
| (wherein R1 is a hydrogen atom or a halogen atom, and R2 is a methyl group or a halogen atom), or a salt thereof.
... |
| Recombinant fusobacterium necrophorum leukotoxin vaccine and preparation thereof | 20090117142 | 20090507 |
| The F. necrophorum gene expressing leukotoxin was sequenced and cloned. The leukotoxin open reading frame (lktA) is part of a multi-gene operon containing 9,726 bp, and encoding a protein containing 3,241 amino acids with an overall molecular weight of 335,956 daltons. The protein encoded by the gene was truncated into five polypeptides having overlapping regions by truncating the full length gene into five different sections and amplifying, expressing, and recovering the protein encoded by each of these sections. Additionally, a region upstream of the gene was sequenced and the polypeptide encoded by that nucleotide sequence was purified and isolated. These polypeptides along with the full length protein are then tested to determine their immunogenicity and protective immunity in comparison to the efficacy of immunization conferred by... |
| Novel process | 20090081735 | 20090326 |
| Novel organisms, including DNA construct host cell combinations, are disclosed. The organisms comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for enzymes which promote the supply of single carbon units for the conversion of dUMP to dTMP. Examples include: dihydrofolate reductase genes e.g. T4 frd; Serine Hydroxymethyltransferase genes e.g. glyA; 3-phosphoglycerate dehydrogenase genes e.g. serA; and THF synthase genes e.g. ADE3. The organisms are used in a biological method of producing thymidine with significantly reduced levels of uridine.
... |
| Native homoethanol pathway for ethanol production in e. coli | 20090082600 | 20090326 |
| A native homoethanol pathway including chromosomal deletions of genes that are competitive with the native homoethanol pathway, and a highly anaerobically expressed pyruvate dehydrogenase operon. Bacteria including the native homoethanol pathway. A method of making a bacteria derivative including a native homoethanol pathway by deleting genes that are competitive with ethanol production pathways, and performing transcriptional gene fusion and highly anaerobically expressing pyruvate dehydrogenase operon. A method of producing ethanol by fermenting bacteria including the native homoethanol pathway with biomass, and producing ethanol. Ethanol produced by the above method.
... |
| Methods and compositions for producing recombinant proteins using a gene for trna | 20090053766 | 20090226 |
| The invention relates to host cells with improved protein expressed properties. The host cells comprise rare tRNA genes within the one or more rRNA operons.
... |
| Stabilized bioactive peptides and methods of identification, synthesis, and use | 20090004696 | 20090101 |
| An intracellular selection system allows screening for peptide bioactivity and stability. Randomized recombinant peptides are screened for bioactivity in a tightly regulated expression system, preferably derived from the wild-type lac operon. Bioactive peptides thus identified are inherently protease- and peptidase-resistant. Also provided are bioactive peptides stabilized by a stabilizing group at the N-terminus, the C-terminus, or both. The stabilizing group can be a small stable protein, such as the Rop protein, glutathione sulfotransferase, thioredoxin, maltose binding protein, or glutathione reductase, an α-helical moiety, or one or more proline residues.
... |
| Bioinformatically detectable group of novel regulatory viral and viral associated oligonucleotides and uses thereof | 20080318210 | 20081225 |
| The present invention relates to a first group of novel viral and human associated oligonucleotides, here identified as Genomic Address Messenger or GAM oligonucleotide, and a second group of novel operon-like viral and human polynucleotides, here identified as Genomic Record or GR polynucleotide. GAM oligonucleotides selectively inhibit translation of known ‘target’ genes, many of which are known to be involved in various viral diseases. Nucleic acid molecules are provided respectively encoding 15484 GAM precursors oligonucleotides, and 699 GR polynucleotides, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting GAM oligonucleotides and GR polynucleotides and specific functions and utilities thereof, for detecting expression of GAM oligonucleotides and GR polynucleotides, and for selectively enhancing and selectively inhibiting translation of the... |
| Expression system, components thereof and methods of use | 20080311618 | 20081218 |
| Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from Pseudomonas putido. Disclosed herein are novel expression systems and components thereof, which involve the development of CymR variants with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variants, fusion proteins incorporating such variants, and their use in the control and expression of polynucleotides.
... |
| Melibiose operon expression system | 20080305526 | 20081211 |
| New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic... |
| Nontypable haemophilus infuenzae virulence factors | 20080292634 | 20081127 |
| The invention relates to a mutation within the sap operon of an avirulent clone of a nontypeable strain of Haemophilus influenzae (NTHi). The invention also relates to the NTHi sap operon genes and the polypeptides encoded by these polynucleotide sequences. The invention also relates to a novel 110 kDa NTHi outer membrane protein and the polynucleotide that encodes this outer membrane protein. Methods of screening for NTHi infection, and treating and preventing NTHi related disorders are also contemplated.
... |
| Vaccination against multiple serotypes of pasteurella multocida | 20080241192 | 20081002 |
| The present invention provides methods for inducing cross-protective immunity against virulent strains of P. multocida in animals such as cattle and poultry. The methods of the invention include administering to an animal a mutant P. multocida strain, whereby the mutant P. multocida strain induces cross-protective immunity against one or more virulent P. multocida strains having serotypes that are different from the serotype of the mutant P. multocida strain. The mutant P. multocida strain will preferably contain one or more mutations that cause the cells to be acapsular and/or attenuated. Exemplary mutations include, e.g., mutations that impair the expression of one or more genes in the P. multocida capsule biosynthetic operon (e.g., phyB, phyA, hyaE, hyaD, hyaC, hyaB, hexD, hexC, hexB, and/or hexA).
... |
| Multiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants | 20080241916 | 20081002 |
| The present invention relates in part to a tobacco chloroplast transformation vector effective for stably transforming a tobacco plant to express a multi-gene operon.
... |
| Method for propagating adenoviral vectors encoding inhibitory gene products | 20080233650 | 20080925 |
| The invention provides a method of propagating an adenoviral vector. The method comprises (a) providing a cell comprising a cellular genome comprising a nucleic acid sequence encoding a tetracycline operon repressor protein (tetR), and (b) contacting the cell with an adenoviral vector comprising a heterologous nucleic acid sequence encoding a toxic protein. The heterologous nucleic acid sequence is operably linked to a promoter and one or more tetracycline operon operator sequences (tetO), and expression of the heterologous nucleic acid sequence is inhibited in the presence of tetR, such that the adenoviral vector is propagated. The invention also provides a system comprising the aforementioned cell and adenoviral vector.
... |
| Rhamnose promoter expression system | 20080206817 | 20080828 |
| The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.
... |
| Bioinformatically detectable group of novel hiv regulatory genes and uses thereof | 20080188428 | 20080807 |
| The present invention relates to a group of novel viral RNA regulatory genes, here identified as “viral genomic address messenger genes” or “VGAM genes”, and as “viral genomic record” or “VGR genes”. VGAM genes selectively inhibit translation of known host target genes, and are believed to represent a novel pervasive viral attack mechanism. VGR genes encode an operon-like cluster of VGAM genes. VGAM and viral VGR genes may therefore be useful in diagnosing, preventing and treating viral disease. Several nucleic acid molecules are provided respectively encoding several VGAM genes, as are vectors and probes, both comprising the nucleic acid molecules, and methods and systems for detecting VGAM genes, and for counteracting their activity.
... |
| Methods and compositions for the treatment and prevention of staphylococcus aureus infections through interference with opuc operon interaction with trap | 20080138332 | 20080612 |
| The bacterial protein OpuCA, an intracellular part of an ABC transporter, has been shown to interact directly with TRAP. The present invention provides methods and compositions directed at interfering with the interaction between OpuCA and TRAP. The resulting inhibition of TRAP advantageously will reduce pathogenesis of all bacteria that utilize this pathway. The present invention further provides methods and compositions directed at interfering with the interaction between TRAP and the extracellular substrate binding protein OpuCC, or the membrane-associated proteins OpuCB and OpuCD, which, like OpuCA, are encoded by the bacterial OpuC operon. Accordingly, the present methods and compositions will be useful in treating diseases caused by such bacteria.
... |
| Expression of foreign cellulose synthase genes in photosynthetic prokaryotes (cyanobacteria) | 20080113413 | 20080515 |
| The present invention includes compositions and methods for making and using cyanobacteria that include a portion of an exogenous cellulose operon sufficient to express cellulose. The compositions and methods of the present invention may be used as a new global crop for the manufacture of cellulose, CO2 fixation, for the production of alternative sources of conventional cellulose as well as a biofuel and precursors thereof.
... |
| Brucella melitensis mutants and methods | 20080107682 | 20080508 |
| Certain attenuated mutants of Brucella, especially B. melitensis, B. abortus, B. suis and B. ovis, when administered to a human or animal trigger a protective immune response such that subsequent challenge with virulent Brucella of the same species does not result in disease or results in much less severe symptoms. Functional inactivation of galE, a virB gene or the operon (ORFs 1087-1090) comprising the gene encoding β-hexosaminidase (BMEI1087) and a lytic murein transglycosylase gene (BMEI1088). A specific example of the attenuated galE mutant which produces a protective immune response is B. melitensis GR024. The specific example of an inactivated ORF1087-1090 operon is B. melitensis GR026; it has an insertion mutation in the promoter region upstream of ORF 1090. Vaccination with live cells of either or both... |
| Production and secretion of glucose in photosynthetic prokaryotes (cyanobacteria) | 20080085520 | 20080410 |
| The present invention includes compositions and methods for making and using an isolated cyanobacterium that includes a portion of an exogenous bacterial cellulose operon sufficient to express bacterial cellulose, whereby the cyanobacterium produces extracellular glucose. The compositions and methods of the present invention may be used as a new global crop for the manufacture of cellulose, CO2 fixation, for the production of alternative sources of conventional cellulose as well as a biofuel and precursors thereof.
... |
| Stable genomic integration of multiple polynucleotide copies | 20080085535 | 20080410 |
| Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; means for Promoter carrying out the methods, resulting cells, and methods for producing a polypeptide of interest using the resulting cells.
... |
| Plastid rrna operon promoter elements for construction of chimeric promoters for transgene expression | 20080086788 | 20080410 |
| Prrn promoter elements for enhancing expression of heterologous molecules, including RNA and proteins in the plastids of higher plants are disclosed.
... |
| Pet family of efflux proteins | 20070298473 | 20071227 |
| The invention provides methods of increasing the production of aromatic carboxylic acids from a host cell via manipulation of the yhcRQP operon encoding a family of efflux proteins. Up-regulation of all or a sub-set of the genes in the yhcRQP were additionally found to enhance tolerance to aromatic carboxylic acids toxicity.
... |
| Methods for altering acetic acid production and enhancing cell death in bacteria | 20070231841 | 20071004 |
| Methods of increasing cultured cell growth yields and/or protein production from bacterial cell cultures are provided. More particularly, mutant bacterial cells having an alteration in the expression or activity of the cidABC operon, a gene therein, or a homolog or a regulator thereof, and methods for reducing acetic acid/acetate production in cultures are provided, as are methods for increasing cultured cell growth yields and/or protein production employing such cells. Methods for enhancing bacterial cell death and methods for identifying agents that increase the susceptibility of bacteria to cell death are also provided.
... |
| Production of isoprenoids | 20070202579 | 20070830 |
| The present invention relates to a process for the production of isoprenoids Coenzyme Q-10 by microorganisms. More particularly, the present invention relates to a process for increased production of Coenzyme Q-10 by microorganisms of the genus Rhodobacter, preferably of the species R. sphaeroides which have been transformed with one or more gene(s) of the mevalonate (mev) operon from a different microorganism, preferably of the genus Paracoccus, more preferably of the species P. zeaxanthinifaciens, whereby the mev operon is mutated leading to an increased coenzyme Q-10 production. Sequences carrying such a mutation as well as a microorganism carrying such a mutated mev operon are also included.
... |
| Use of canthin-6-one, plant extracts containing same and derivatives thereof in the treatment of trypanosomiases | 20070149461 | 20070628 |
| The invention concerns the use of canthin-6-one from plant extracts containing same, in particular canthin-6-one in the form of a Zanthoxylum chiloperone of the angustifolium variety, and some of its derivatives for making a medicine for the treatment of trypanosomiases, in particularly for treating Chagas disease.
... |
| Bioinformatically detectable group of novel regulatory genes and uses thereof | 20070134655 | 20070614 |
| The present invention relates to a first group of novel genes, here identified as genomic address messenger or GAM genes, and a second group of novel operon-like genes, here identified as genomic record or GR genes. GAM genes selectively inhibit translation of known ‘target’ genes, many of which are known to be involved in various diseases. Nucleic acid molecules are provided respectively encoding 8607 GAM genes, and 1096 GR genes, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting GAM and GR genes and specific functions and utilities thereof, for detecting expression of GAM and GR genes, and for selectively enhancing and selectively inhibiting translation of the respective target genes thereof.
... |
| Bioinformatically detectable group of novel vaccinia regulatory genes and uses thereof | 20070077553 | 20070405 |
| The present invention relates to a group of novel viral RNA regulatory genes, here identified as “viral genomic address messenger genes” or “VGAM genes”, and as “Viral genomic record” or “VGR genes”. VGAM genes selectively inhibit translation of known host target genes, and are believed to represent a novel pervasive viral attack mechanism. VGR genes encode an “operon”-like cluster of VGAM genes. VGAM and viral VGR genes may therefore be useful in diagnosing, preventing and treating viral disease. Several nucleic acid molecules are provided respectively encoding several VGAM genes, as are vectors and probes, both comprising the nucleic acid molecules, and methods and systems for detecting VGAM genes, and for counteracting their activity.
... |
| Promoter and plasmid system for genetic engineering | 20070065867 | 20070322 |
| This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.
... |
| Bioinformatically detectable group of novel regulatory bacterial and bacterial associated oligonucleotides and uses thereof | 20070042982 | 20070222 |
| The present invention relates to a first group of novel bacterial and human associated oligonucleotides, here identified as Genomic Address Messenger or GAM oligonucleotide, and a second group of novel operon-like bacterial and human polynucleotides, here identified as Genomic Record or GR polynucleotide. GAM oligonucleotides selectively inhibit translation of known ‘target’ genes, many of which are known to be involved in various bacterial diseases. Nucleic acid molecules are provided respectively encoding 6444 GAM precursors oligonucleotides, and 726 GR polynucleotides, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting GAM oligonucleotides and GR polynucleotides and specific functions and utilities thereof, for detecting expression of GAM oligonucleotides and GR polynucleotides, and for selectively enhancing and selectively inhibiting translation of the... |
| Bioinformatically detectable group of novel regulatory viral and viral associated oligonucleotides and uses thereof | 20070042381 | 20070222 |
| The present invention relates to a first group of novel viral and human associated oligonucleotides, here identified as “Genomic Address Messenger” or “GAM” oligonucleotide, and a second group of novel operon-like viral and human polynucleotides, here identified as “Genomic Record” or “GR” polynucleotide. GAM oligonucleotides selectively inhibit translation of known “target” genes, many of which are known to be involved in various viral diseases. Nucleic acid molecules are provided respectively encoding 1,655 viral and 105,537 human GAM precursor oligonucleotides, and 190 viral and 14,813 human GR polynucleotides, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting GAM oligonucleotides and GR polynucleotides and specific functions and utilities thereof, for detecting expression of GAM oligonucleotides and GR polynucleotides, and for... |
| Bioinformatically detectable group of novel regulatory oligonucleotides and uses thereof | 20070042380 | 20070222 |
| The present invention relates to a first group of novel oligonucleotides, here identified as “Genomic Address Messenger” or “GAM” oligonucleotide, and a second group of novel operon-like polynucleotides, here identified as “Genomic Record” or “GR” polynucleotide. GAM oligonucleotides selectively inhibit translation of known “target” genes, many of which are known to be involved in various diseases. Nucleic acid molecules are provided respectively encoding 122,764 GAM oligonucleotides and their respective precursors, and 18602 GR polynucleotides, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting GAM oligonucleotides and GR polynucleotides and specific functions and utilities thereof, for detecting expression of GAM oligonucleotides and GR polynucleotides, and for selectively enhancing and selectively inhibiting translation of the respective target genes thereof.
... |
| Bioinformatically detectable group of novel vaccinia regulatory genes and uses thereof | 20070031823 | 20070208 |
| The present invention relates to a group of novel viral RNA regulatory genes, here identified as “viral genomic address messenger genes” or “VGAM genes”, and as “genomic record” or “GR” genes. VGAM genes selectively inhibit translation of known host target genes, and are believed to represent a novel pervasive viral attack mechanism. GR genes encode an operon-like cluster of VGAM genes. VGAM and viral GR genes may therefore be useful in diagnosing, preventing and treating viral disease. Several nucleic acid molecules are provided respectively encoding several VGAM genes, as are vectors and probes, both comprising the nucleic acid molecules, and methods and systems for detecting VGAM genes, and for counteracting their activity.
... |
| Bioinformatically detectable group of novel regulatory bacterial and bacterial associated oligonucleotides and uses thereof | 20070031843 | 20070208 |
| The present invention relates to a first group of novel bacterial and human associated oligonucleotides, here identified as “Genomic Address Messenger” or “GAM” oligonucleotide, and a second group of novel operon-like bacterial and human polynucleotides, here identified as “Genomic Record” or “GR” polynucleotide. GAM oligonucleotides selectively inhibit translation of known “target” genes, many of which are known to be involved in various bacterial infections. Nucleic acid molecules are provided respectively encoding 21,916 bacterial and 6,100 human GAM precursor oligonucleotides, and 6,056 bacterial and 430 human GR polynucleotides, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting GAM oligonucleotides and GR polynucleotides and specific functions and utilities thereof, for detecting expression of GAM oligonucleotides and GR polynucleotides, and for... |
| Sequence of the photorhabdus luminescens strain tt01 genome and uses | 20070020625 | 20070125 |
| The present invention relates to the genomic sequence and to nucleotide sequences encoding polypeptides of Photorhabdus luminescens. The present invention further relates to polypeptides involved in operons involved in the biosynthesis of antibiotics or toxins, as well as polypeptides with activity of the antibiotic or toxin. Uses of the aforementioned polypeptides in pesticides, bactericides, or fungicides is provides. In addition, the present invention provides vectors, cells, or animals containing the sequences of the present invention.
... |
| Altered glyoxylate shunt for improved production of aspartate-derived amino acids and chemicals | 20070015261 | 20070118 |
| The invention provides microbial strains possessing improved properties for production of aspartate-derived amino acids and chemicals. Methods of making such strains are provided. These methods include altering expression of the aceBAK operon, the glcB gene, or both. Alteration of expression may be accomplished through increased transcription, relief from native transcriptional control, and/or other means. Replacement of native promoters for these genes is also contemplated; for instance, their native promoters may be replaced by the tac promoter (Ptac).
... |
| Biosensor for small molecule analytes | 20060292581 | 20061228 |
| A biosensor device for detecting small molecules analytes is provided. The device employs a first class of molecules, e.g., protein that binds to both the analyte and a second class of molecules, e.g., nucleic acid. The binding of the protein to the analyte and nucleic acid can be mutually exclusive, and the presence of analyte in a sample results in a detectable displacement of protein from nucleic acid. Alternatively, binding of the protein to the nucleic acid can depend on the presence of analyte in the sample. In a specific embodiment, either the protein or nucleic acid is immobilized on a solid phase support. An arsenic detection system is exemplified. An ArsR binding sequence from the E. coli ars operon is immobilized on a gold-plated surface.... |
| Inhibitors of autoinducer transporters | 20060269951 | 20061130 |
| The present invention relates to the discovery of the lsr operon, the genes therein, and the polypeptides encoded by these genes. The present invention also includes strains with altered expression levels of the polypeptides encoded by the genes and the lsr operon relative to wild type cells. In some embodiments, the strains express a transporter that transports an autoinducer into the cell at a level higher than that of wild type cells. The present invention also includes methods for identifying compounds that modulate the transport of the autoinducer into cells.
... |
| Bioinformatically detectable group of novel viral regulatory genes and uses thereof | 20060257851 | 20061116 |
| The present invention relates to a first group of novel genes, here identified as genomic address messenger or VGAM genes, and a second group of novel operon-like genes, here identified as viral genomic record or VGR genes. VGAM genes selectively inhibit translation of known ‘target’ genes, many of which are known to be involved in various diseases. Nucleic acid molecules are provided respectively encoding 1560 VGAM genes, and 205 VGR genes, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting VGAM and VGR genes and specific functions and utilities thereof, for detecting expression of VGAM and VGR genes, and for selectively enhancing and selectively inhibiting translation of the respective target genes thereof.
... |
| Method for producing l-amino acid by fermentation | 20060216796 | 20060928 |
| L-threonine or L-isoleucine is produced by culturing a bacterium which belongs to the genus Escherichia and has an ability to produce L-threonine or L-isoleucine, and wherein expression of a threonine operon is directed by its native promoter, and from which at least a leader sequence and an attenuator are deleted, in a medium and collecting the L-threonine or L-isoleucine from the medium.
... |
| Solvent for chromogenic substrate solution | 20060172369 | 20060803 |
| The present invention relates to a non-toxic dipolar solvent for chromogenic substrate for detecting presence of lacZ gene and/or gene activity, which comprises a stabilizing amount of a solubilizing agent. The present invention also relates to a method for inducing lac operon in screening assay, comprising the step of contacting an agar plate with at least one essential oil in a concentration sufficient to induce the lac operon. The present invention further relates to a method for detecting the presence of bacteria, comprising the step of contacting an agar plate with at least one essential oil in a concentration sufficient to induce detection of the bacteria.
... |
| Production of coenzyme q-10 | 20060154349 | 20060713 |
| Improved process for the preparation of CoQ10 by fermentation of microorganisms of the genus Rhodobacter transformed with the mevalonate operon of Paracoccus zeaxanthinifaciens.
... |
| Stabilized bioactive peptides and methods of identification, synthesis, and use | 20060099571 | 20060511 |
| An intracellular selection system allows screening for peptide bioactivity and stability. Randomized recombinant peptides are screened for bioactivity in a tightly regulated expression system, preferably derived from the wild-type lac operon. Bioactive peptides thus identified are inherently protease- and peptidase-resistant. Also provided are bioactive peptides stabilized by a stabilizing group at the N-terminus, the C-terminus, or both. The stabilizing group can be a small stable protein, such as the Rop protein, glutathione sulfotransferase, thioredoxin, maltose binding protein, or glutathione reductase, an α-helical moiety, or one or more proline residues.
... |
| Uropathogenic e. coli d-serine detoxification operon | 20060099629 | 20060511 |
| Disclosed are methods of detecting uropathogenic E. coli genes that are differentially expressed in response to D-serine. Also disclosed are methods of characterizing bacterial isolates from clinical samples based on the ability to metabolize D-serine.
... |
