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Operon-related patent applications - as published by the U.S. Patent and Trademark Office (USPTO).


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Modified bacteria for the production of bioalcohol
International Centre For Genetic Engineering And Biotechnology (icgeb)
September 14, 2017 - N°20170260549

The present invention provides a modified bacterial strain capable of fermenting both hexose and pentose sugars for production of bioalcohol wherein a promoter of pyruvate dehydrogenase operon (pdh) is replaced with a promoter of a gene that is expressed under anaerobic conditions. The present invention further provides a method of obtaining modified bacterial strain capable of fermenting both hexose and ...
Methods for sequential screening with co-culture based detection of metagenomic elements conferring heterologous metabolite secretion
Metamixis Biologics, Inc.
August 10, 2017 - N°20170226503

The present invention relates to methods associated with metagenomic screening for metabolite induced elements (mies) and the subsequent use of the mies in screening metagenomic libraries to identify metabolic pathways and pathway components in one or more partial or complete operons. In one aspect the method may be an iterative approach to metagenomic screening which involves substrate and product selection.
Stable genomic integration of multiple polynucleotide copies
Novozymes A/s
August 03, 2017 - N°20170218381

Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (orf) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; resulting cells, and methods for producing a polypeptide of interest ...
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Recombinant cells and methods for nonphosphorylative metabolism
Regents Of The University Of Minnesota
May 18, 2017 - N°20170137855

This disclosure describes methods for screening heterologous coding regions (e. G., a single gene coding region, and operon, or other gene cluster) for the ability to genetically modify a host cell to perform nonphosphorylative biosynthesis of 2,5-dioxopentanoate and downstream derivatives thereof (e. G., 1,4-butanediol, glutamate, or glutaconate. This disclosure further describes recombinant cells modified to increase nonphosphorylative biosynthesis of 2,5-dioxopentanoate ...
Rhamnose promoter expression system
Regents Of The University Of Minnesota
April 20, 2017 - N°20170107525

The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence ...
Methods for producing microcompartments
The Regents Of The University Of California
April 20, 2017 - N°20170107523

To produce a bacterial microcompartment shell, or a designed shell based on naturally occurring bacterial microcompartment shells in a new host organism, a synthetic operon is constructed that contains the desired shell protein genes and translation efficiency is controlled by host specific ribosomal binding sites. Proteins or other molecules can be encapsulated in the microcompartment shells by various methods described ...
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Stabilizied bioactive peptides and methods of identification, synthesis and use
Peptide Biosciences, Inc.
March 16, 2017 - N°20170074858

An intracellular selection system allows screening for peptide bioactivity and stability. Randomized recombinant peptides are screened for bioactivity in a tightly regulated expression system, preferably derived from the wild-type lac operon. Bioactive peptides thus identified are inherently protease- and peptidase-resistant. Also provided are bioactive peptides stabilized by a stabilizing group at the n-terminus, the c-terminus, or both. The stabilizing group ...
Mutated lux operon sequences
Bioglow, L.l.c.
February 16, 2017 - N°20170044505

The present disclosure relates to mutated genes of the lux operon and their use in producing autoluminescent plants and bacteria exhibiting improved light output.
Production and use of bacterial histamine
Biogaia Ab
January 26, 2017 - N°20170020936

A method is provided of selecting specific probiotic lactic acid bacteria producing histamine and the use of such strains for beneficial effects for mammals. The method includes selecting a lactic acid bacterial strain for use in the local production of histamine in a mammal, and further comprises screening bacteria for the presence of an active histidine operon and selecting a ...
Enhanced protein expression
Danisco Us Inc.
November 03, 2016 - N°20160319288

The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include gram positive microorganisms, such as bacillus species, having a genetic alteration that modifies activity of a protein encoded by the ykf operon and ...
Method for propagating adenoviral vectors encoding inhibitory gene products
Genvec, Inc.
October 20, 2016 - N°20160304882

The invention provides a method of propagating an adenoviral vector. The method comprises (a) providing a cell comprising a cellular genome comprising a nucleic acid sequence encoding a tetracycline operon repressor protein (tetr), and (b) contacting the cell with an adenoviral vector comprising a heterologous nucleic acid sequence encoding a toxic protein. The heterologous nucleic acid sequence is operably linked ...
Production of magnetic nanoparticles in recombinant host cells
Ludwig-maximilians-universität München
October 13, 2016 - N°20160296643

The present invention relates to a recombinant host cell, comprising in its genome preferably heterologous gene expression cassettes capable of being expressed in the host cell, the gene expression cassettes encoding a nucleic acid sequence encompassing at least 80% of the full-length sequence of the mamab operon, the mamgdfc operon, and the tmms6 operon of a magnetotactic alphaproteobacterium, respectively, and preferably ...
Methods of using salmonella enterica presenting c. jejuni n-glycan or derivatives thereof
Eidge-noessische Technische Hochschule Zuriche
July 28, 2016 - N°20160213770

The present invention relates to salmonella enterica comprising at least pgl operon of campylobacter jejuni or a functional derivative thereof and presenting at least one n-glycan of campylobacter jejuni or n-glycan derivative thereof on its cell surface and, in particular, to medical uses and pharmaceutical compositions thereof as well as methods for treating and/or preventing campylobacter and optionally salmonella ...
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Method for producing l-leucine, l-valine, l-isoleucine, alpha-ketoisovalerate, alpha-keto-beta-methylvalerate, or alpha-ketoisocaproate using recombinant corynebacteria that contain ...
Evonik Degussa Gmbh
April 28, 2016 - N°20160115506

The invention relates to a process for the production of amino acids and keto acids using microorganisms, in which a promoter inducible by propionate makes possible the regulated expression of certain genes.
Bacterial mutants with improved transformation efficiency
Novozymes A/s
April 28, 2016 - N°20160115490

Provided herein are bacillus mutants having improved transformation efficiency, comprising a disruption of an endogenous epsa-o operon. Also described are methods for producing the mutants, methods for generating transformants using the mutants, and methods for producing a polypeptide or fermentation product using the mutants.
Strain having enhanced l-valine productivity and l-valine production method using the same
Novozymes A/s
April 21, 2016 - N°20160108444

The present invention relates to a novel l-valine-producing strain which is transformed so as to strengthen the expression of l-valine operon by having a nucleotide sequence, for coding a leader peptide in a regulatory region of ilvbn operon, entirely deleted, or partially deleted or substituted. In addition, the present invention relates to a method for producing l-valine using the novel ...
Allelic disorders caused by mutations in trpv4
Novozymes A/s
April 14, 2016 - N°20160102356

The present invention provides methods, kits, and compositions for detecting mutations in transient receptor potential cation channel, subfamily v, member 4 (trpv4). In particular, mutations are detected in trpv4 to detect diseases such as scapuloperoneal spinal muscular atrophy (spsma) and hereditary motor and sensory neuropathy type iic (hmsn iic) or charcot-marie-tooth disease type 2c (cmt2c).
Resistance against hyaloperonospora parasitica in diplotaxis tenuifolia
Novozymes A/s
April 14, 2016 - N°20160100544

The invention relates to a rucola plant of the species diplotaxis tenuifolia which may comprise genetic determinant b and optionally a that leads to expression of resistance against at least hyaloperonospora parasitica isolates pp0615 and pp1248, which genetic determinant b is obtainable from a diplotaxis tenuifolia plant which may comprise said genetic determinant, representative seed of which was deposited with ...
Attenuated chlamydia vaccine
The United States Of America,as Represented By The Secretary, Department Of Health & Human Services
January 21, 2016 - N°20160015798

The invention provides vectors, attenuated pathogens, compositions, methods, and kits for use in preventing or treating infection by an infectious pathogen, especially chlamydia trachomatis. The vectors comprise the plasmid encoded ppg genes from chlamydia, ppg1, ppg2, ppg3, ppg5, ppg6, ppg7 and/or ppg8, but lack ppg4, a regulator of virulence associated genes. The application also provides attenuated pathogens, especially chlamydia, ...
Methods and apparatus for cell-free microfluidic-assisted biosynthesis
Massachusetts Institute Of Technology
January 07, 2016 - N°20160002611

A trans-disciplinary system for cell-free biosynthesis includes a cell-free transcription-translation (tx-tl) tool and modular, generalizable microfluidic architectures. Both components of the system are independently functional and are combinable into a cell-free biosynthesis platform. In the first component, modular plasmid libraries are used to program bacterial cell-free tx-tl systems. Each plasmid holds one gene or operon, and all the genes are ...
Method for bacterial species identification and strain typing
The Regents Of The University Of Michigan
December 31, 2015 - N°20150376685

The present disclosure describes a method for identifying a strain or species of bacteria using a single locus sequence typing technique. The single locus useful in the method is the promoter region of the 16s rrna operon. The method is useful to identify infectious bacteria in a subject, to identifying contaminants in a food source, as well as strain typing ...
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