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Synthesis of n-fmoc protected deoxy nucleosides, ribo nucleosides, modified deoxy and ribo nucleosides, and phosphoramidites, and their use in oligonucleotide synthesis
This invention relates to synthesis of novel -N-FMOC protected nucleosides, succinates, phosphoramidites, corresponding solid supports that are suitable for oligo deoxy nucleosides and RNA oligonucleotide synthesis. Our discovery using N-FMOC as nucleoside base protecting group, which is highly base labile protecting group is a novel approach to obtain highest purity oligonucleotides....

Emissive lamps comprising metal clusters confined in molecular sieves
The present invention concerns an illumination system comprising radiation source that when operational emits radiation in wave length range below 280 nm to an emission element that comprises an assembly of oligo atomic metal clusters confined in molecular sieve for converting invisible radiation emitted by a radiation source at room temperature...

Single molecule real time sequencer, nucleic acid analyzer and single molecule real time sequencing method
An object of the present invention relates to selectively control an extension reaction within a desired area in a substrate. In the present invention, an oligo probe arranged with a caged compound at the terminal thereof is immobilized to a reaction field area in the substrate. After pouring a reaction solution...

Polymeric coupling agents and pharmaceutically-active polymers made therefrom
(iv) n is selected from 2-50. The compounds are useful as biomaterials, particularly, providing antibacterial activity in vivo. Also provided are biological coupling agents useful as intermediates in the preparation of the pharmaceutically-active polymeric compounds. (iii) m is 1-40; and ...

Lysis and reverse transcription for mrna quantification
The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA comprising the steps of a) lysis of a cellular sample which is supposed to contain the target RNA with a lysis buffer comprising between 0.2 M and 1 M Guanidine Thiocyanate, b) diluting the...

Phosphoramidites for synthetic rna in the reverse direction, efficient rna synthesis and convenient introduction of 3'-end ligands, chromophores and modifications of synthetic rna
The present invention provides building blocks and methods for synthesizing very pure RNA in a form that can efficiently be modified at the 3′ end. Reverse RNA monomer phosphoramidites have been developed for RNA synthesis in 5′→3′ direction, leading to very clean oligo synthesis that allows for the introduction of various...

Avian influenza virus antigen, and booster immunization method for avian influenza vaccine in combination with mucosal adjuvant which is effective through oral administration
When ELISA antibody titer dropped in chickens intramuscularly inoculated with an oil vaccine, recombinant HA and NP antigens prepared using gene recombinant technology were orally administered in combination with synthetic oligo CpG as a mucosal adjuvant to the chickens. Even when the immune response induced with the oil vaccine was impaired,...

Glycosylated glp-1 peptide
Oligosaccharide chain added GLP-1 peptides are more stable in blood and more active in controlling blood-sugar levels than GLP-1 peptides without added oligosaccharides. Oligosaccharide chain added GLP-1 peptides having GLP-1 activity include at least one or at least two amino acids each substituted with an oligosaccharide chain added amino acid in...

Method for production of cdna library having reduced content of cdna clone derived from highly expressed gene
A method for efficiently constructing a cDNA library having a reduced content of cDNA clones derived from a highly expressed gene is provided. According to the method for constructing a cDNA library using a double-stranded DNA primer having oligo dT and mRNA as a template, the proportion of cDNA clones derived...

Targeted oligonucleotide compositions for modifying gene expression
The invention comprises compositions and methods for modifying gene expression. Modified oligos of the invention restore the lost function of let-7 wild type miRNA molecules that are prevented from silencing target genes by mutations occurring within their binding sites. Administration of a particular modified oligo (SEQ ID NO: 22) leads to...

Structure, synthesis, and applications for oligo phenylene ethynylenes
Novel compounds generally referred to herein as cationic oligomeric phenylene ethynylenes (OPEs), methods of synthesizing OPEs and various uses for the OPEs are described. The compounds can be synthesized in both symmetrical (S-OPE) and non-symmetrical (N-OPE) forms. Suitable uses include sensor and biocidal applications. Reusable structures incorporating the OPEs that are...

Compositions and methods for treating hypophosphatasia
The present invention provides compositions and methods for use in enzyme replacement therapy. The inventors disclose a method of producing membrane bound enzymes in an active soluble form by eliminating the glycosylphosphatidylinositol (GPI) membrane anchor. In particular the inventors disclose a soluble active form of the membrane bound enzyme TNSALP which...

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