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|| List of recent Oleaginous Yeast-related patents
|Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oilseed plants and oleaginous yeast|
The present invention relates to fungal Δ-15 fatty acid desaturases that are able to catalyze the conversion of linoleic acid (18:2, la) to alpha-linolenic acid (18:3, ala). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, dna constructs comprising the desaturase genes, and recombinant host plants and microorganisms expressing increased levels of the desaturases are described.
|High eicosapentaenoic acid producing strains of yarrowia lipolytica|
Engineered strains of the oleaginous yeast yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (epa, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of epa.
|Process for separation of renewable materials from m icroorganisms|
Methods of separating renewable materials, such as lipids, from microorganisms, such as oleaginous yeasts, may include conditioning cell walls of the microorganisms to form, open or enlarge pores, and removing at least a portion of the renewable material through the pores. These methods may result in delipidated microorganisms with cell walls that are substantially intact and with mesopores.
|Mutant acyl-coa:lysophosphatidylcholine acyltransferases|
Mutant acyl-coa:lysophosphatidylcholine acyltransferases [“lpcats”] having the ability to convert acyl-coa+1-acyl-sn-glycero-3-phosphocholine to coa+1,2-diacyl-sn-glycero-3-phosphocholine (ec 188.8.131.52) are disclosed herein. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding mutant lpcats, along with a method of making long chain polyunsaturated fatty acids [“pufas”] using these mutant lpcats in oleaginous yeast, are also disclosed..
|Renewable chemicals and fuels from oleaginous yeast|
The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase.
|Process for the production of lipids from biomass|
Process for the production of lipids from biomass comprising polysaccharides. The biomass is subjected to acid hydrolysis to obtain a solid phase and an aqueous phase.
|Production of polyunsaturated fatty acids in oleaginous yeasts|
The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (la) to γ-linolenic acid (gla); α-linoleic acid (ala) to stearidonic acid (sta); gla to dihomo-γ-linoleic acid (dgla); sta to eicosatetraenoic acid (eta); dgla to arachidonic acid (ara); eta to eicosapentaenoic acid (epa); dgla to eta; epa to docosapentaenoic acid (dpa); and ara to epa have been introduced into the genome of yarrowia for synthesis of ara and epa..
|High arachidonic acid producing strains of yarrowia lipolytica|
Engineered strains of the oleaginous yeast yarrowia lipolytica capable of producing greater than 10% arachidonic acid (ara, an ω-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of ara.
|Mutant delta-5 desaturases and their use in making polyunsaturated fatty acids|
The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [dgla; 20:3 ω-6] to arachidonic acid [ara; 20:4 ω-6] and/or eicosatetraenoic acid [eta; 20:4 ω-3] to eicosapentaenoic acid [epa; 20:5 ω-3] and which possess at least one mutation within the hpgg motif of the cytochrome b5-like domain. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“pufas”] using these mutant Δ5 desaturases in oleaginous yeast, are disclosed..
|Production of carotenoids in oleaginous yeast and fungi|
The present invention provides systems for producing engineered oleaginous yeast or fungi that express carotenoids. .
|Delta 17 desaturase and its use in making polyunsaturated fatty acids|
The present invention relates to Δ17 desaturases, which have the ability to convert ω-6 fatty acids into their ω-3 counterparts (i.e., conversion of arachidonic acid [20:4, ara] to eicosapentaenoic acid [20:5, epa]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ17 desaturases along with a method of making long-chain polyunsaturated fatty acids (pufas) using these Δ17 desaturases in oleaginous yeast are disclosed..
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Oleaginous Yeast topics: Oleaginous Yeast, Arachidonic Acid, Polyunsaturated Fatty Acid, Eicosapentaenoic Acid, Fatty Acid, Saturated Fat, Unsaturated Fat, Recombinant, Fatty Acids, Yarrowia Lipolytica, Linolenic Acid, Nucleic Acid, Carotenoids, Carotenoid, Microfiltration
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