|| List of recent Nucleotide-related patents
| Compositions and methods of gene silencing in plants|
Compositions and methods for inducing gene silencing events in plants are disclosed. The compositions typical include a polynucleotide encoding an mirna target sequence operably linked to a sequence of from a target gene, cdna or mrna, or fragment thereof.
The Board Of Trustees Of The University Of Illinois
| Novel antifungal proteins and methods of use|
Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for novel variants of antipathogenic polypeptides generated through dna shuffling that exhibit improved antipathogenic activity.
Pioneer Hi Bred International Inc
| Ribonucleotide reductase inhibitors and methods of use|
Provided herein are novel compounds that inhibit ribonucleotide reductase (rr) by binding to rrm2 and interfering with the activity of the rrm1/rrm2 holoenzyme, as well as methods of synthesizing these novel compounds. The compounds may be used to inhibit rr activity and to treat various conditions associated with rrm2 expression, such as for example certain cancer types, mitochondrial diseases, or degenerative diseases..
City Of Hope
| Microfluidic reactors for oligonucleotide synthesis|
The present disclosure generally pertains to systems and methods for the chemical synthesis of micro-quantities of oligonucleotides or other chemical molecules. The system includes a reusable glass micro-reactor containing a paramagnetic solid support, a magnet, an electronic drive controller and an optical spectroscopy system capable of driving a plurality individual reactors.
| Treatment of erythropoietin (epo) related diseases by inhibition of natural antisense transcript to epo|
Oligonucleotide compounds modulate expression and/or function of erythropoietin (epo) polynucleotides and encoded products thereof. Methods for treating diseases associated with erythropoietin (epo) comprise administering one or more oligonucleotide compounds designed to inhibit the epo natural antisense transcript to patients..
| G protein-coupled purinergic receptor gpr17 mediates orexigenic effects of foxo1 in agrp neurons|
G protein-coupled receptor (gpcr) gpr17 expressed in hypothalamic agouti-related peptide-expressing (agrp) neurons increases appetite and glucose tolerance and insulin sensitivity. By contrast, increasing gpr17 reduced glucose tolerance and increased appetite.
The Trustees Of Columbia University In The City Of New York
| Anti-fatty acid synthase polypeptide and use thereof|
The present invention relates to a polypeptide that is capable of inhibiting transcription and expression of fatty acid synthase (fas) and the polynucleotides encoding therefor, as well as the use thereof. Specifically, the present invention relates to a polypeptide that can inhibit the transcription and expression of fas at the molecular level, the cellular level and in vivo, and can therefore prevent the overexpression of fas.
Tianjin Toptech Bio-science & Technology Co., Ltd.
| Peptide-conjugated oligonucleotide therapeutic and making and using same|
In which the peptide is a homing peptide which directs the conjugate to cells of a particular type, antisense is an antisense oligonucleotide having a sequence selected to provide sequence-specific inhibition of the target protein, and hbl is a heterobifunctional linker having reactivity towards amino and sulfhydryl groups.. .
| Huwentoxin-iv variants and methods of use|
The present invention relates to huwentoxin-iv variants, polynucleotides encoding them, methods of making and using the foregoing, and methods of alleviating pain with peptide inhibitors of nav1.7.. .
Janssen Biotech, Inc.
| Enzyme composition for dna end repair, adenylation, phosphorylation|
Enzyme compositions and their method of use that provide ready-to-use master mixtures. The compositions comprise a modified thermophilic dna polymerase lacking 5′-3′ and 3′-5′ exonuclease activity premixed with t4 dna polymerase, klenow fragment and t4 polynucleotide kinase and all other necessary components, including reaction buffer and nucleoside triphosphates, required to perform dna blunting, phosphorylation, and single nucleotide extension reactions in one tube and in two steps.
Thermo Fisher Scientific Baltics Uab
Screening the detection of clostridium difficile
The invention concerns a screening method for the detection of clostridium difficile in a sample, wherein said method comprises the detection of conserved target regions in the clostridium genome through the binding of at least one oligonucleotide probe to said target site.. .
University College Cardiff Consultants Limited
Methods, systems, computer readable media, and kits for sample identification
A method for sequencing a polynucleotide sample having a barcode sequence includes: introducing a series of nucleotides to the polynucleotide sample according to a predetermined order of nucleotide flows; obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample; and resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-correcting code that is (i) designed based on and adapted for use with the predetermined order of nucleotide flows, and (ii) capable of distinguishing any codeword in the error-correcting code from the other codewords in the error-correcting code in the presence of zero, one, and two errors.. .
Life Technologies Corporation
High expression zymomonas promoters
Synthetic, derivative promoters for expression of chimeric genes in zymomonas, zymobacter, and related bacteria were created. The promoters have a c to t change in the nucleotide at position 90 of the z.
E I Du Pont De Nemours And Company
Methods and compositions for inactivating glutamine synthetase gene expression
Disclosed herein are methods and compositions for inactivating a glutamine synthetase (gs) gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins..
Sangamo Biosciences, Inc.
Methods and compositions for producing active vitamin k-dependent proteins
The present invention provides a method of identifying a human subject having increased or decreased sensitivity to warfarin, comprising detecting in the subject the presence of a single nucleotide polymorphism in the vkor gene, wherein the single nucleotide polymorphism is correlated with increased or decreased sensitivity to warfarin, thereby identifying the subject having increased or decreased sensitivity to warfarin.. .
The University Of North Carolina At Chapel Hill
Process for production of recombinant proteins as a soluble form
A target protein is prepared as soluble protein using a recombinant protein expression system. An expression vector is used that includes (1) an expression-inducible promoter sequence; (2) a first coding sequence including a polynucleotide coding for a polypeptide that is represented by the formula (z)n; and (3) a second coding sequence that includes a polynucleotide that codes for a target protein.
Genes encoding cellulase for hydrolyzing guar fracturing fluids under extreme well conditions
Polynucleotide sequences encoding a thermostable cellulase and directing its increased expression are provided, and hydraulic fracturing compositions comprising such thermostable cellulase.. .
Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
The present invention is directed to methods and compositions for adding tails of specific lengths to a substrate polynucleotide. The invention also contemplates methods and compositions for immobilization of tailed substrates to a solid support.
Swift Biosciences, Inc.
Nucleic acid construct and use of the same
An expression unit for the expression of e1b, wherein the expression unit comprises a promoter, a nucleotide sequence coding for e1b, and a 3′utr, wherein the promoter is operatively linked to the nucleotide sequence coding for e1b, wherein the 3′utr comprises 30 or less than 30 exonic enhancer elements (eses), preferably 20 or less than 20 exonic enhancer elements (eses), and wherein the 3′ utr is a non-viral 3′ utr.. .
Phosphorothioate oligonucleotide-labeling of white blood cells
The present invention provides labeling and capture reagents that comprise phosphorothioate oligonucleotides odn). The ps-odn bind to all white blood cells (leukocytes) in an indiscriminative fashion and enable the labeling, capture, or concentration of leukocytes in a manner that preserves the antigenic integrity of the cells.
Becton, Dickinson And Company
The present invention provides novel compositions, methods and apparatus for dna sequencing that can be performed, e.g., in a two-electrode chamber. The present invention also provides a method for sequencing a nucleic acid comprising immobilizing a plurality of complexes comprising a target nucleic acid, a primer nucleic acid, and a polymerase onto a surface, contacting the surface with a plurality of charged particles comprising a nucleotide phosphate by applying an electric field, reversing the electric field to transport unbound charged particles away from the surface, and detecting the incorporation of a nucleotide phosphate into a single molecule of the primer nucleic acid..
Pacific Biosciences Of California, Inc.
Detection of target nucleic acid sequences by pto cleavage and extension assay
The present invention relates to the detection of a target nucleic acid sequence by a ptoce (pto cleavage and extension) assay. The present invention detects a target nucleic acid sequence in which the pto (probing and tagging oligonucleotide) hybridized with the target nucleic acid sequence is cleaved to release a fragment and the fragment is hybridized with the cto (capturing and templating oligonucleotide) to form an extended duplex, followed by detecting the presence of the extended duplex.
Nucleoside-triphosphate conjugate and methods for the use thereof
The invention relates to a novel method for the enzymatic marking of nucleic acid chains (target sequences) with nucleotide conjugates. Under reaction conditions, said nucleotide conjugates are able to bind to a target sequence, and can be incorporated into the complementary growing strand by way of a polymerase.
Myostatin inhibition for enhancing muscle and/or improving muscle function
The present invention relates to methods for inhibiting myostatin, a regulator of muscle mass, for muscle enhancement (including inducing hypertrophy and/or hyperplasia) as well as improving muscle function (including decreasing atrophy and/or increasing endurance, force and/or strength). Some of the methods involve delivering genes to cells using gene delivery or other delivery techniques known in the art in order to inhibit myostatin.
Nationwide Children's Hospital
Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmrna molecules.. .
Moderna Therapeutics, Inc.
Lipid-derived neutral nanoparticles
Disclosed herein are novel lipids and liposomal compositions prepared using such compounds and related methods of neutralizing or otherwise modifying such liposomal compositions. The lipids described herein are useful for example, as liposomal vehicles to facilitate the delivery of encapsulated polynucleotides to target cells and the subsequent transfection of such target cells.
Shire Human Genetic Therapies, Inc.
The invention relates to immunostimulatory oligonucleotides and methods of using immunostimulatory oligonucleotides to induce an antigen-specific immune response. The invention further relates to a vaccine that comprises an immunostimulatory oligonucleotide and an antigen, and comprises a pharmaceutically acceptable carrier.
Coley Pharmaceutical Group, Inc.
Parainfluenza virus 5 based vaccines
The present invention provides safe, stable, efficacious, and cost-effective vaccines based on viral expression vectors that include a parainfluenza virus 5 (piv5) genome including a heterologous nucleotide sequence expressing a heterologous polypeptide. In some embodiments, the heterologous nucleotide sequence is inserted closer to the leader than between the hemagglutinin-neuroaminidase (hn) gene and the large rna polymerase protein (l) gene of the piv5 genome.
University Of Georgia Research Foundation, Inc.
Neisseria meningitidis antigens and compositions
The invention provides proteins from neisseria meningitidis, including the amino acid sequences and the corresponding nucleotide sequences. The proteins are predicted to be useful antigens for vaccines and/or diagnostics..
J. Craig Venter Institute, Inc.
Pathogenic phlebovirus isolates and compositions and methods of use
Described herein are the clinical and laboratory characteristics of two patients bitten by ticks and infected with a unique member of the genus phlebovirus (family bunyaviridae) with a proposed name of heartland virus (hrtlv). Provided herein are nucleotide and amino acid sequences of the phlebovirus isolates, primers and probes that specifically hybridize with the phlebovirus isolates, and antibodies specific for the phlebovirus proteins.
The Government Of The United States Of America As Represented By The Secretary Of The Department Of
The invention herein provides isolated antibodies that bind to vegf. The invention further provides methods of making anti-vegf antibodies, and polynucleotides encoding anti-vegf antibodies..
Anti-activin a antibodies and uses thereof
The disclosure provides compositions and methods relating to or derived from anti-activin a binding proteins, including antibodies. In particular embodiments, the disclosure provides fully human, humanized, and chimeric anti-activin a antibodies that bind human activin a, activin a-binding fragments and derivatives of such antibodies, and activin a-binding polypeptides comprising such fragments.
Cross-reactive staphylococcus aureus antibody
The subject relates to a cross-neutralizing antibody comprising at least one polyspecific binding site that binds to alpha-toxin (hla) and at least one of the bi-component toxins of staphylococcus aureus, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated conformational epitope recognized by a specific cross-neutralizing antibody.. .
Arsanis Biosciences Gmbh
Prebiotic composition or pharmaceutical composition synthesized from catalytic domains producing highly alpha-1,2 branched dextran
The invention relates to an isolated polypeptide with an glycosyl transferase enzymatic activity for producing dextrans with .alpha.(1.fwdarw.2) sidechains, comprising at least one region for bonding to glucan and a catalytically active region situated beyond the region bonding to glucan. The invention further relates to polynucleotides coding for said enzymes and vectors containing the same..
Institut National Des Sciences Appliquees (insa)
Rice gene, gs3, exerting primary control over grain length and grain weight
The present invention relates to an isolated major gene gs3 which regulates grain weight and grain length in the rice and the cloning of said gene. The dna sequence of gs3 gene is as shown in seq id no.
Huazhong Agricultural University
Cell modified in the expression of a nucleotide sugar transporter
The present invention provides for a genetically modified eukaryotic host cell comprising (a) a gene encoding a first nucleotide sugar transporter (nst) operably linked to a promoter, wherein the gene and/or the promoter is heterologous to the cell, and/or (b) a native gene encoding a second nts is disrupted and/or a promoter of the native gene is disrupted. Such modified cells can be altered in the production of polysaccharide and/or glycopeptides.
The Regents Of The University Of California
Gh61 polypeptide variants and polynucleotides encoding same
The present invention relates to gh61 polypeptide variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants..
Polynucleotides and polypeptides for increasing desirable plant qualities
Polynucleotides and isolated polypeptides, nucleic acid constructs comprising the isolated polynucleotides, transgenic plants expressing same and methods of using same for increasing abiotic stress tolerance, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or nitrogen use efficiency of a plant are disclosed.. .
Isolated polynucleotides encoding a map65 polypeptide and methods of using same for increasing plant yield
Provided are methods of increasing yield, biomass, growth rate, vigor, and/or abiotic stress tolerance of a plant by expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence at least 80% identical to seq id no: 27 or 868; or an exogenous polynucleotide encoding a polypeptide at least 80% identical to seq id no: 132 (map65 from arabidopsis).. .
Polynucleotides, polypeptides encoded thereby, and methods of using same for increasing abiotic stress tolerance, biomass and/or yield in plants expressing same
Provided are isolated polynucleotides comprising a nucleic acid sequence at least 80% identical to seq id no:619, 617, 606, 615, 629, 1-36, 40, 41, 43-45, 49, 52-56, 58, 113-343, 351, 354-358, 605, 607-614, 616, 618, 620-628, 630-638, 642, 645, 650, 651, 670, or 671. Also provided are nucleic acid constructs comprising same, isolated polypeptides encoded thereby, transgenic cells and transgenic plants comprising same and methods of using same for increasing abiotic stress tolerance, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or nitrogen use efficiency of a plant..
Isolated polynucleotides and polypeptides, and methods of using same for increasing yield of plants
Provided are isolated polynucleotides and nucleic acid constructs which comprise a nucleic acid sequence at least 80% identical to a nucleic acid sequence selected form the group consisting of seq id nos: 1-219, 367-5628, 9688-9700, and 9709-9752; and isolated polypeptides which comprise an amino acid sequence at least 80% homologous to an amino acid sequence selected from the group consisting of seq id nos: 220-366, 5629-9400, 9701-9708, and 9753-9796. Also provided are transgenic cells and plants expressing same and methods of using same for increasing yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, nitrogen use efficiency, and/or abiotic stress of a plant..
Nucleic acid molecules and other molecules associated with transcription in plants and uses thereof for plant improvement
Polynucleotides useful for improvement of plants are provided. In particular, polynucleotide sequences are provided from plant sources.
Monsanto Technology Llc
Plant genome modification using guide rna/cas endonuclease systems and methods of use
Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide rna/cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed.
Pioneer Hi-bred International Inc