|| List of recent Nucleotide-related patents
|Compositions and methods to control insect pests|
Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a coleopteran plant pest or a diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant.
|Plant expression constructs comprising and uses thereof|
Methods of expressing a molecule of interest in a plant are disclosed. One method comprises contacting roots of the plant in a solution comprising at least one geminivirus based expression construct so as to allow the at least one geminivirus based expression construct to be absorbed by the roots, the expression construct comprising a polynucleotide encoding the molecule of interest, and further the expression construct being capable of systemic symptomless spread in a plant host, thereby expressing a molecule of interest in a plant.
|Method for assembly of nucleic acid sequence data|
The present invention relates to a method for assembly of nucleic acid sequence data comprising nucleic acid fragment reads into (a) contiguous nucleotide sequence segment(s), comprising the steps of: (a) obtaining a plurality of nucleic acid sequence data from a plurality of nucleic acid fragment reads; (b) aligning said plurality of nucleic acid sequence data to a reference sequence; (c) detecting one or more gaps or regions of non-assembly, or non-matching with the reference sequence in the alignment output of step (b); (d) performing de novo sequence assembly of nucleic acid sequence data mapping to said gaps or regions of non-assembly; and (e) combining the alignment output of step (b) and the assembly output of step (d) in order to obtain (a) contiguous nucleotide sequence segment(s). In addition, a corresponding program element or computer program for assembly of nucleic acid sequence data and a sequence assembly system for transforming nucleic acid sequence data comprising nucleic acid fragment reads into (a) contiguous nucleotide sequence segment(s) is provided..
|Methods for detecting a polymorphism in the nfkb1 gene promoter|
The present invention discloses a functional relationship between a recognized disease condition and a polymorphism in the nucleotide factor kappa b promoter (nfkb1). This relationship provides a platform for methods of altering promoter activity and for determining similar relationships between specific pathologies and identified polymorphisms.
|Modulation of pre-mrna using splice modulating oligonucleotides as therapeutic agents in the treatment of disease|
The present invention encompasses a class of compounds known as splice modulating oligonucleotides (smos) that modulate pre-mrna splicing, thereby affecting expression and functionality of a specific protein in a cell. The present invention further provides compositions and methods for modulating pre-mrna splicing using a smo of the invention to abrogate disease-causing mutations in a protein.
|Modified polynucleotides for treating protein deficiency|
The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmrna molecules.. .
|Means for inhibiting the expression of orc-1|
The present invention is related to a nucleic acid molecule comprising a double-stranded structure, whereby the double-stranded structure comprises a first strand and a second strand, whereby the first strand comprises a first stretch of contiguous nucleotides and said first stretch is at least partially complementary to a target nucleic acid, and whereby the second strand comprises a second stretch of contiguous nucleotides and said second stretch is at least partially complementary to the first stretch, whereby the first stretch comprises a nucleic acid sequence which is at least complementary to a nucleotide core sequence of the nucleic acid sequence according to seq id no: 1, whereby the nucleotide core sequence comprises the nucleotide sequence from nucleotide positions 1755 to 1763 of seq id no: 1; from nucleotide positions 1904 to 1912 of seq id no: 1; from nucleotide positions 1905 to 1913 of seq id no: 1; from nucleotide positions 2548 to 2556 of seq id no: 1; whereby the first stretch is additionally at least partially complementary to a region preceding the 5′ end of the nucleotide core sequence and/or to a region following the 3′ end of the nucleotide core sequence.. .
|Oligonucleotides for modulation of target rna activity|
The present invention relates to oligonucleotides for modulation of target rna activity. Thus, the invention provides oligonucleotides that bind to microrna binding sites of target rna.
|Fluid processing device for oligonucleotide synthesis and analysis|
The present teachings provide a fluid processing device adapted to produce different oligomers in a plurality of respective reaction sites. The fluid processing device can comprise a first manifold for delivering reactants to the plurality of reaction sites, and a second manifold for removing waste from, and optionally delivering wash fluid to, the plurality of reaction sites.
|Novel glyphosate-n-acetyltransferase (gat) genes|
Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds.
|Apparatus and method for electrical detection of oligonucleotides through pore blockades|
Systems and methods for specific nucleic acid (na) sequence detection that do not rely on polymerase chain reaction (pcr) for target sequence amplification and do not require any special reagents other than a complementary sequence capture probe conjugated to spherical beads.. .
Isolated polynucleotides comprising a dcx mini-promoters are provided. The mini-promoter may be operably linked to an expressible sequence, e.g.
|Methods for expressing polypeptides in hyperthermophiles|
Provided herein are genetically engineered archaea. A genetically engineered archaea includes a heterologous polynucleotide that has a promoter operably linked to a coding region, where the coding region encodes a polypeptide having optimal activity below the optimum growth temperature (topt) of the genetically engineered archaeon.
|Polypeptides having peroxygenase activity and polynucleotides encoding same|
The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Methods of increasing secretion of polypeptides having biological activity|
The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.. .
|Enhancing spinosyn production with oxygen binding proteins|
The invention describes the integration of polynucleotides into chromosomal dna of s. Spinosa species, which are useful for the production of insecticides, integrants thereof, and also to the use of the integrants.
|Polypeptides having cellobiohydrolase activity and polynucleotides encoding same|
Provided are isolated polypeptides having cellobiohydrolase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains..
|Host cell for the production of a compound of interest|
The present invention relates to a recombinant host cell for the production of a compound of interest. The invention further relates to a method for the production of such host cell.
|Zcytor19 polynucleotides, polypeptides, antibodies and methods of use|
Novel polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed for zcytor19, a novel class ii cytokine receptor. The polypeptides may be used within methods for detecting ligands that stimulate the proliferation and/or development of hematopoietic, lymphoid and myeloid cells in vitro and in vivo.
|Cloned glucagon-like peptide-2 receptors|
The invention relates to nucleotides and amino acid sequences encoding glucagon-like peptide-2 receptors, recombinant host cells transformed with such nucleotides, and methods of using the same in drug screening and related applications.. .
|Diagnosis of sepsis and systemic inflammatory response syndrome|
The present invention relates a method for the diagnosis, prediction or risk stratification for mortality and/or disease outcome of a subject that has or is suspected to have sepsis, comprising determining the presence and/or level of antitrypsin (att) or fragments thereof in a sample taken from said subject and/or determining the presence and/or level of transthyretin (ttr) or fragments thereof, wherein the presence and/or level of att and/or ttr or fragments thereof is correlated with an increased risk of mortality and, wherein said increased risk of mortality and/or poor disease outcome is given if the level of att is below a certain cut-off value and/or the level of fragments thereof is above a certain cut-off value and/or said increased risk of mortality and/or poor disease outcome is given if the level of ttr is below a certain cut-off value and/or the level of fragments thereof is below a certain cut-off value. The invention relates in general to the use of att and/or ttr or its fragments for the diagnosis of sepsis, and to nucleotides of seq id no.
|Isoforms of the human sst5 receptor originated by alternative splicing and oligonucleotide pairs to detect them by pcr|
The present invention is referred to two human nucleic 5 acids that comprise sequences encoding two new isoforms of the human somatostatin receptor type 5 originated by alternative splicing, named sst5b and sst5c, with possible involvement in tumor processes. In addition, the invention is referred to oligonucleotide pairs allowing 10 their differential detection in several tissues using the pcr technique..
|Pcr methods for characterizing the 5' untranslated region of the fmr1 and fmr2 genes|
This disclosure relates to methods of determining the presence and position of agg or interruptor elements within a trinucleotide (for example, cgg) repeat region, and to methods of determining the number of repeats present in this region, by amplifying a set of products with a set of primers of which at least one comprises a portion of the cgg repeat region, and resolving the products to produce a representation of product size and abundance.. .
|Dna recombination junction detection|
The present invention provides methods, compositions and kits for detecting the presence or absence of an integrated insertion polynucleotide.. .
|Method for detecting the presence of a nucleic acid in a sample|
An automated method for detecting the presence of a nucleic acid in a sample, where the method is performed within a housing of a self-contained, stand-alone analyzer. The method includes purifying the nucleic acid after it has been immobilized on a magnetically-responsive solid support.
|Compositions and methods for detecting allelic variants|
The present invention provides compositions, methods, and kits for discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides compositions, methods, and kits for determining the presence and/or level (e.g., quantitating) of rare (e.g., mutant) allelic variants, such as single nucleotide polymorphisms (snps) or nucleotide insertions or deletions, in samples comprising abundant (e.g., wild-type) allelic variants with high sensitivity and/or specificity.
|Reagents, methods, and libraries for bead-based sequencing|
The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage.
|Methods for characterizing a device component based on a contrast signal to noise ratio|
The general concept of using a nanopore for dna sequencing is to electrophoretically drive a polymer (e.g. Single stranded dna) through a nanopore under aqueous conditions, and identify each individual monomer (e.g.
|Brucella phage polynucleotides and uses thereof|
An isolated polynucleotide is disclosed which comprises a nucleic acid sequence of a brucella phage, the nucleic acid sequence being specific to the brucella phage and comprising a sequence selected from the group consisting of seq id nos: 387-393. An exemplary polynucleotide sequence is one which comprises at least 100 consecutive nucleotides of a nucleic acid sequence as set forth in seq id no: 396.
|Divalent-metal coated nanoparticles for delivery of compositions into the central nervous system by nasal insufflation|
The compositions and methods of the disclosure particularly target the divalent metal transporter expressed on olfactory nerve terminals to transport divalent cation-coated or cation-containing nanoparticles to all regions of brain. It has been found that such divalent cation-containing nanoparticles, including those nanoparticles comprising manganese have affinity for the metal transport receptor proteins.
|Peptides that target dorsal root ganglion neurons|
The present invention concerns methods and compositions that employ peptides that target dorsal root ganglion (drg) neurons. In particular, the peptides are used to target therapeutic agents, such as proteins, liposomes, or viral particles comprising therapeutic polynucleotides, to one or more peripheral neuropathies or neuropathic pain, for example.
|Non-hemolytic llo fusion proteins and methods of utilizing same|
The present invention provides recombinant proteins or peptides comprising a mutated listeriolysin o (llo) protein or fragment thereof, comprising a substitution or internal deletion of the cholesterol-binding domain or a portion thereof, fusion proteins or peptides comprising same, nucleotide molecules encoding same, and vaccine vectors comprising or encoding same. The present invention also provides methods of utilizing recombinant proteins, peptides, nucleotide molecules, and vaccine vectors of the present invention to induce an immune response to a peptide of interest..
|Rab7 gtpase inhibitors and related methods of treatment|
This invention relates to compounds and their use as inhibitors or activators of rab7 gtpase to treat or prevent the onset of rab 7 gtpase-associated disorders such as neuropathies, cancer, metabolic diseases of bone and lipid storage. The invention is also applicable to infectious diseases where rab7 is inactivated or its protein-protein interactions are modulated to facilitate intracellular survival of pathogens.
|Anti-cd33 antibodies and methods for treatment of acute myeloid leukemia using the same|
The present invention relates to antibodies that bind cd33. More particularly, the invention relates to anti-cd33 antibodies, fragments and homologues of these antibodies, humanized and resurfaced versions of these antibodies, functional equivalents and improved versions of these antibodies, immunoconjugates and compositions comprising these antibodies, and the uses of same in diagnostic, research and therapeutic applications.
|Sorghum plants having a mutant polynucleotide encoding the large subunit of mutated acetohydroxyacid synthase protein and increased resistance to herbicides|
A sorghum seed comprising in its genome at least one polynucleotide encoding a polypeptide having an alanine to threonine substitution at position 93 of the sorghum ahas protein large subunit. The plant has increased resistance to one or more herbicides, for example from the imidazolinone group, as compared to wild-type sorghum plants.
|Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same|
The present invention relates to isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Drought tolerant plants and related constructs and methods involving genes encoding ferredoxin family proteins|
Isolated polynucleotides and polypeptides and recombinant dna constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant dna constructs, and methods utilizing these recombinant dna constructs. The recombinant dna construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a ferredoxin family protein..
|Gene associated with non-biological stress resistance, and transformed plant|
The present invention relates to a composition for enhancing non-biological stress resistance in plants and a composition for accelerating germination. A nucleotide sequence of the present invention is involved in the resistance against the drying stresses in plants, and a transformed plant in which the nucleotide sequence is overexpressed has prominent resistance against various kinds of non-biological stress, including drought stress.
|Axmi218, axmi219, axmi220, axmi226, axmi227, axmi228, axmi229, axmi230 and axmi231 delta-endotoxin genes and methods for their use|
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided.
|Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics in response to cold|
Methods and materials for modulating cold tolerance levels in plants are disclosed. For example, nucleic acids encoding cold tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells.
|Non-human animals with modified immunoglobulin heavy chain sequences|
Non-human animals, e.g., mammals, e.g., mice or rats, are provided comprising an immunoglobulin heavy chain locus that comprises a rearranged human immunoglobulin heavy chain variable region nucleotide sequence. The rearranged human immunoglobulin heavy chain variable region nucleotide sequence may be operably linked to a heavy or light chain constant region nucleic acid sequence.
|Surprisal data reduction of genetic data for transmission, storage, and analysis|
A method, computer product, and computer system of reducing an amount of data representing a genetic sequence of an organism, comprising: a computer comparing nucleotides of the genetic sequence of the organism to nucleotides from a reference genome, to find differences where nucleotides of the genetic sequence of the organism which are different from the nucleotides of the reference genome; the computer using the differences to create and store surprisal data in a repository, the surprisal data comprising a starting location of the differences within the reference genome, and the nucleotides from the genetic sequence of the organism which are different from the nucleotides of the reference genome, discarding sequences of nucleotides that are the same in the genetic sequence of the organism and the reference genome.. .
|Codon optimization of a synthetic gene(s) for protein expression|
The present disclosure is related to a method of optimization of a nucleotide coding sequence coding for an amino acid sequence, wherein the nucleotide coding sequence is optimized for expression in a host cell. The present disclosure also relates to system for optimizing a nucleotide coding sequence coding for an amino acid sequence, wherein the nucleotide coding sequence is optimized for expression in a host cell..