This page is updated frequently with new Nucleotide-related patent applications.
|Transgenic animal model of mood disorders|
A non-human transgenic animal having a polynucleotide encoding a ptn polypeptide, which polynucleotide is operably linked to a promoter, wherein said transgenic animal has greater than wild-type expression of the ptn polypeptide in at least one brain region, as well as related vectors, methods of producing transgenic animals, in vitro and in vivo screening methods for potential therapeutic agents, and methods for treating and diagnosing neuropsychiatric illnesses, particularly anxiety and depression, are disclosed.. .
Brainco Biopharma, S.l.
|Detection ntrk3 fusion|
A polynucleotide, which is a novel causative gene for cancer, is elucidated, and, based on this finding, provided are a method for detecting the polynucleotide, or a polypeptide encoded by the polynucleotide; a kit and a primer set for the detection; a method for screening an inhibitor of the polypeptide; and a pharmaceutical composition for treating a cancer containing the inhibitor. In the detection method of the present invention, an ntrk3 fusion protein, or a fusion gene encoding the fusion protein, or an etv6 fusion protein, or a fusion gene encoding the fusion protein, in a sample derived from the digestive system obtained from a subject, is detected..
Japanese Foundation For Cacer Research
|Markers for pre-cancer and cancer cells and the method to interfere with cell proliferation therein|
A novel family of human mitochondrial rnas, referred to as chimeric rnas, which are differentially expressed in normal, pre-cancer and cancer cells, are described. Oligonucleotides targeted to the chimeric rnas are provided.
Andes Biotechnologies S.a.
|Compositions and methods for detecting rare sequence variants|
In some aspects, the present disclosure provides methods for identifying sequence variants in a nucleic acid sample. In some embodiments, a method comprises identifying sequence differences between sequencing reads and a reference sequence, and calling a sequence difference that occurs in at least two different circular polynucleotides, such as two circular polynucleotides having different junctions, as the sequence variant.
|Cleavable competitor polynucleotides|
The invention relates to polynucleotide combinations and their use in allele-specific enrichment, amplification, and detection. The disclosure also provides methods to multiplex various target dna molecules in a single tube with high sensitivity and specificity.
Swift Biosciences, Inc.
|Rna amplification and oligonucleotide library preparation|
This disclosure describes, in one aspect, a method of amplifying an rna template. Generally, the method includes synthesizing an oligonucleotide from the rna template, isolating at least a portion of the oligonucleotide, and subjecting the isolated product to treatment with an rnase and/or glycosylase..
Regents Of The University Of Minnesota
|Template switch-based methods for producing a product nucleic acid|
Provided are methods of producing a product nucleic acid. The methods include combining a template deoxyribonucleic acid (dna), a polymerase, a template switch oligonucleotide, and dntps into a reaction mixture.
Clontech Laboratories, Inc.
|Compositions and methods for performing hybridizations with separate denaturation of the sample and probe|
The invention provides methods and compositions for separately denaturing a probe and target in hybridization applications. The invention may, for example, eliminate the use of or reduce the dependence on formamide in hybridization applications.
Dako Denmark A/s
|Quantification of mutant alleles and copy number variation using digital pcr with nonspecific dna-binding dyes|
Methods and reagents for performing digital pcr for detection and quantification of mutant alleles and copy number variation are disclosed. In particular, the invention relates to methods using a nonspecific dna-binding dye, which produces a fluorescent signal that increases in intensity according to the number of base-pairs present in the pcr amplicon product.
The Board Of Trustees Of The Leland Stanford Junior University
|Fad-conjugated glucose dehydrogenase gene|
An object of the present invention is to provide: a novel gene (polynucleotide) encoding an fad-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an fad-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: x1-x2-x3-x4-x5-x6 (wherein x1 and x2 independently represent an aliphatic amino acid residue; x3 and x6 independently represent a branched amino acid residue; and x4 and x5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others..
Ikeda Food Research Co., Ltd.
Synthesis of l-nucleic acids by means of an enzyme
The present invention is related to a method for adding one or more l-nucleotides to the 3′end of a first l-nucleic acid, wherein the method comprises the step of reacting the one or more l-nucleotides with the first l-nucleic acid in the presence of a protein comprising a mutant enzymatic activity exhibiting moiety, wherein the enzymatic activity is capable of adding one or more l-nucleotides to the 3′ end of the first l-nucleic acid, wherein the mutant enzymatic activity exhibiting moiety comprises an amino acid sequence, wherein the amino acids of the amino acid sequence are d-amino acids, wherein the mutant enzymatic activity exhibiting moiety is a variant of an enzymatic activity exhibiting moiety, wherein the enzymatic activity exhibiting moiety consists of an amino acid sequence according to seq id no: 15 and wherein the amino acids of the amino acid sequence according to seq id no: 15 are d-amino acids, wherein the amino acid sequence of the mutant enzymatic activity exhibiting moiety differs from the amino acid sequence of the enzymatic activity exhibiting moiety consisting of an amino acid sequence according to seq id no: 15 at least at one amino acid position, preferably at three amino acid positions, and/or wherein the amino acid sequence of the mutant enzymatic activity exhibiting moiety is a truncated form of an amino acid sequence according to seq id no: 15, and wherein the amino acid sequence of the mutant enzymatic activity exhibiting moiety is different from an amino acid sequence according to any of seq id nos 15 to 22 and 51.. .
Noxxon Pharma Ag
Fungal gene library by double split-marker integration
The present invention relates to a method for site-specific chromosomal integration of a gene library in a filamentous fungal host cell and a polynucleotide construct suitable for this purpose.. .
Pesticidal genes and methods of use
Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, dna constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides.
Plant promoter for transgene expression
This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a panicum virgatum metallothionein-like gene (mtl). Some embodiments relate to a promoter from a panicum virgatum metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences..
Dow Agrosciences Llc
Plant promoter for transgene expression
This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a brachypodium distachyon metallothionein-like gene (mtl). Some embodiments relate to a promoter from a brachypodium distachyon metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences..
Dow Agrosciences Llc
Targeted gene modification using oligonucleotide-mediated gene repair
The invention provides to improved methods for the modification of genes in plant cells, and plants and seeds derived therefrom. More specifically, the invention relates to the increased efficiency of targeted gene mutation by combining gene repair oligonucleotides with approaches that enhance the availability of components of the target cell gene repair mechanisms..
Cibus Europe B.v.
Compositions and methods for altering flowering and plant architecture to improve yield potential
The present invention provides recombinant dna constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic ft protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic ft protein.
Monsanto Technology Llc
Genetic transformation of bifidobacteria
The present invention concerns a method for genetically transforming a bifidobacterium strain comprising a step of methylation of a shuttle vector in an e. Coli or a gram-positive bacterium strain by two type ii dna methyltransferases from a bifidobacterium: a methyltransferase enzyme that methylates the adenine base at position 4 of the nucleotide sequence rtcagg and a methyltransferase enzyme that methylates the cytosine base at position 4 of the nucleotide sequence ggwcc.
University College Cork - National University Of Ireland, Cork
Nucleic acid inducing rna interference modified for preventing off-target, and use thereof
The present invention relates to an rna interference-inducing nucleic acid and the use thereof, and more particularly to an rna interference-inducing nucleic acid comprising at least one single strand of double strands, the at least one single strand comprising a modification substituted to a spacer, which is unable to form a base pair, in the 5′ end or the 3′ end region. The rna interference-inducing nucleic acid of the present invention is a novel modified form of nucleotide provided to prevent off-target effects, offering a method to selectively repress target gene expression.
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Antisense oligonucleotides (odn) against smad7 and uses thereof in medical field
The invention relates to antisense oligonucleotidic sequences (odn) against smad7 suitably modified, and their uses in medical field as therapeutic biological agents, in particular in the treatment of chronic inflammatory bowel disease, such as crohn's disease and ulcerative colitis.. .
Nogra Pharma Limited
Methods for treatment of wound healing utilizing chemically modified oligonucleotides
The present invention relates to rnai constructs with improved tissue and cellular uptake characteristics and methods of use of these compounds in dermal and fibrotic applications. Aspects of the invention provide nucleic acid molecules for the prophylactic treatment of wounding to reduce scarring.
Rxi Pharmaceuticals Corporation
High temperature selection of nucleotide-supported carbohydrate vaccines and resulting glycosylated oligonucleotides
The invention relates to an oligonucleotide including one or more modified nucleoside bases having the structure -b-l-a wherein for each of the modified nucleosides a is independently a monosaccharide or oligosaccharide, l is a linker molecule, and b is independently a pyrimidine or pyridine base linked to the sugar-phosphate backbone of the oligonucleotide; and wherein the oligonucleotide binds specifically to a carbohydrate-binding monoclonal antibody with an affinity of less than 100 nm. Immunogenic conjugates that include the oligonucleotide, and pharmaceutical compositions that include the oligonucleotide or the immunogenic conjugate are also disclosed.
Immunotherapy of cancer
Immunogenic modulators and compositions comprising oligonucleotide agents capable of inhibiting suppression of immune response by reducing expression of one or more gene involved with an immune suppression mechanism.. .
Drug for disease caused by expression of periostin except eye disease, and use thereof
The present invention is intended to provide a novel molecule that inhibits the expression of the periostin gene that is effective in treatment of diseases caused by the expression of periostin except for eye diseases. A drug for a disease caused by the expression of periostin except for an eye disease includes, as an expression inhibitory sequence for the periostin gene, a nucleic acid molecule including a nucleotide that has a base sequence represented by any one of seq id nos: 1 to 19.
Aqua Therapeutics Co., Ltd.
Tools and methods using mirna 182, 96 and/or 183 for treating pathologies
The present inventions relates to isolated nucleic acid molecules comprising a nucleotide sequence coding for mi rna-182 (uuuggcaaugguagaacucacacu or ugguucuagacuugccaacua), mirna-96 (uuuggcacuagcacauuuuugcu or aaucaugugcagugccaauaug) and/or mi rna-183 (uauggcacugguagaauucacu or gugaauuaccgaagggccauaa) for use in treating or ameliorating a ciliopathy and/or a photoreceptor dysfunction.. .
Friedrich Miescher Institute For Biomedical Research
Polynucleotide barcode generation
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
10x Genomics, Inc.
Novel peptides and uses thereof
The present disclosure relates to novel peptides and their uses including, proline-rich peptides that are useful for displaying a protein of interest at the surfaces of a host cell such as a yeast. Polynucleotides, proteins, vectors and host cells that comprise or encode the novel proline-rich peptides, including libraries comprising such polynucleotides, proteins, vectors and/or host cells that comprise or encode novel proline-rich peptides are provided.
Coexpression of cas9 and trex2 for targeted mutagenesis
A composition used in targeted mutagenesis is provided, which includes a first expression cassette comprising a nucleotide sequence which encodes a cas9 endonuclease; a second expression cassette comprising a nucleotide sequence which encodes a guide rna sequence, wherein the guide rna sequence is complementary to a target genome nucleotide sequence in a cell; and a third expression cassette comprising a nucleotide sequence which encodes a trex2 exonuclease (trex2) gene. The first, second, and third expression cassettes may be a part or a portion of one or more expression vectors..
City Of Hope
Polynucleotide encoding psicose epimerase and producing psicose using the same
A psicose 3-epimerase, a polynucleotide encoding the enzyme, a recombinant vector carrying the polynucleotide, a recombinant cell harboring the recombinant vector, and use thereof are provided.. .
Improved beta-glucosidase enzymes for increased biomass saccharification
Described herein are beta-glucosidase enzymes that have improved beta-glucosidase activity compared to a control beta-glucosidase enzyme. The improved beta-glucosidase enzymes are useful for converting a cellulosic biomass to fermentable sugars such as glucose.
New dna cleavage enzyme
There is provided an enzyme which has an activity of cleaving a phosphodiester bond of deoxyribonucleotide having a damaged base and deoxyribonucleotide adjacent to the 5′ side of the deoxyribonucleotide in dna strands which contain the damaged base as a reagent or the like for manipulating a gene, and further provided a method of removing a damaged base from dna strands using the enzyme.. .
Kyushu University, National University Corporation
Mutant rb69 dna polymerase
Provided herein are mutant dna-dependent polymerases which are derived from, or otherwise related to, wild type rb69 dna polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides.
Life Technologies Corporation
Mutant transaminases as well as methods and uses relating thereto
The present invention relates to a mutant transaminase with increased transaminase activity relative to the wild-type transaminase, a fusion protein comprising the transaminase, a polynucleotide coding for the transaminase, a host cell comprising the polynucleotide, mutant transaminase and/or fusion protein, a method of producing an amine with the mutant transaminase or fusion protein and the use of the mutant transaminase or fusion protein for the production of an amine.. .
Hoffmann-la Roche Inc.
Processes for producing polyunsaturated fatty acids in transgenic organisms
The present invention relates to polynucleotides from ostreococcus lucimarinus which code for desaturases and elongases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides, and to the polypeptides encoded by the polynucleotides.
Basf Plant Science Gmbh
In vivo generating diversity in a protein scaffold
A transgenic non-human animal is provided. In certain embodiments, the animal comprises a genome comprising an immunoglobulin heavy chain locus comprising: a) a transcribed gene encoding a fusion protein comprising, from n-terminus to c-terminus: i.
Crystal Bioscience Inc.
Cell-based anti-cancer compositions with reduced toxicity and methods of making and using the same
Methods of treating an individual who has been diagnosed with cancer of a mucosal tissue or preventing such cancer in an individual at elevated risk are disclosed as are nucleic acid molecules that comprise a nucleotide sequence that encode proteins that recognize at least one epitope of an intestinal cancer antigen or cns cancer antigen and t cells comprising such nucleic acid molecules.. .
Production cell line enhancers
The present invention relates to discovery of the ectopic expression of edem2 in a production cell to improve the yield of a useful multi-subunit protein. Thus, the present invention provides for production cell lines, such as the canonical mammalian biopharmaceutical production cell—the cho cell, containing recombinant polynucleotides encoding edem2.
Regeneron Pharmaceuticals, Inc.
Modified nucleic acid molecules and uses thereof
The present disclosure provides modified nucleosides, nucleotides, and nucleic acids, and methods of using them.. .
Moderna Therapeutics, Inc.
De novo synthesized gene libraries
De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein.
Twist Bioscience Corporation
Fabrication of patterned arrays
Provided herein are methods and compositions for the fabrication of patterned arrays, such as nucleotide arrays. The methods and compositions are suited for the transfer and reorientation of array components..
Centrillion Technology Holdings Corporations
Polypeptide, nucleotide sequence thereof, and using the same for preventing dna synthesis and inhibiting cell proliferation
A polypeptide represented by seq id no: 1 and a method for treatment of a tumor and another disease associated with abnormal cell proliferation are described. The method includes administering a pharmaceutically-effective amount of the polypeptide to a patient in need thereof..
Wuhan Yicheng Biotech. Inc.
Method for detecting pathogens using microbeads conjugated to biorecognition molecules
A method and system are provided for the simultaneous detection and identification of multiple pathogens in a patient sample. The sample is combined with microbeads, which have been injected with quantum dots or fluorescent dye and conjugated to pathogen-specific biorecognition molecules, such as antibodies and oligonucleotides.
Biosensor comprising waveguide
The invention relates to an optical device (110) and a corresponding detection apparatus (100) that may for example be used for monitoring the replication of nucleotide sequences at a surface. In a preferred embodiment, the optical device (110) comprises a waveguide substrate (130) with a wiregrid (140) on a bottom surface (132), wherein apertures (141) of the wiregrid are in at least one direction (x) smaller than a characteristic wavelength (λ) of input light (il).
Koninklijke Philips N.v.
Influence of genotype on susceptibility to treatment with fish oil
The present invention relates to methods for assessing an individual's susceptibility to treatment of an inflammatory disease with a dietary supplement. The invention describes the recognition that the sensitivity of an individual to the inflammation suppressing effects of fish oil on tnf-α production is linked both to genetic variation encoded by, or associated with, tnf-α, lt-α and/or il-6 single nucleotide polymorphisms and to the inherent level of production of tnf-α by cells..
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Conformational probes and methods for sequencing nucleic acids
This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template..
Methods for genotyping
The present invention provides for methods for discriminating between alleles at polymorphic positions in a genome. In general the methods employ allele specific extension of oligonucleotides that are complementary to one of the alleles at the 3′ end of the oligonucleotide.
Nucleic acid detection and quantification
The present invention relates to methods and uses for the detection or quantification of newly-synthesized double-stranded target nucleic acid molecules in a sample during quantitative real-time polymerase chain reaction (qpcr) amplification. According to the invention, an intercalating dye recognizing double-stranded dna molecules with higher affinity than single-stranded dna molecules and a fluorophore-labeled oligonucleotide-probe being sequence specific for a target nucleic acid molecule are simultaneously employed, thus enabling quantification a specific target and total amount of a mixed nucleic acid population, and enabling assessing the cause of suboptimal pcr performance..
Bioprobes and methods of use thereof
Disclosed are biomolecule based bioprobes that exhibit improved water solubility and mono layer-forming properties with substantially little or no aggregation that can appreciably interfere with binding of the bioprobes to a target nucleotide. The bioprobes may be used in conjunction with a suitable reporter system to detect very small quantities of biological markers.
The Governing Council Of The University Of Toronto
Methods for full-length amplification of double-stranded linear nucleic acids of unknown sequences
An adaptor for use in amplifying all linear, double-stranded nucleic acid molecules of unknown sequences in a sample is disclosed. The adaptor consists of: (1) the first oligonucleotide (p-oligo) with a phosphate at the 5′ end and without an additional thymine nucleotide at the 3′ end; and (2) the second oligonucleotide (t˜oligo) with an extra 3′-t and without a 5′-phosphate.
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Codon-optimized gene for mutated shrimp luciferase and use thereof
There has been a demand for a codon-optimized gene for the mutated catalytic domain of oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and escherichia coli. There has also been a demand for a substrate coelenterazine analogue showing a higher activity than that of native 19 kda protein.
Compositions comprising a beta-glucosidase polypeptide and methods of use
The present compositions and methods relate to a beta-glucosidase from melanocarpus albomyces, polynucleotides encoding the beta-glucosidase, and methods of making and/or using the same. Formulations containing the beta-glucosidase are suitable for use in numerous applications, including hydrolyzing lignocellulosic biomass substrates..
Danisco Us Inc.
Enrichment of oils with polyunsaturated fatty acids
The present invention relates to isolated phospholipid:diacylglycerol acyltransferases (pdat) and polynucleotide sequences encoding the pdat enzymes; polynucleotide constructs, vectors and host cells incorporating the polynucleotide sequences; and methods of producing and using same. Also provided are transformed cells and transgenic plants with enhanced oil accumulation and quality..
Plant regulatory elements and uses thereof
The present invention provides dna molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants. Transgenic plants, plant cells, plant parts, and seeds comprising the dna molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use..
Monsanto Technology Llc
Peanut-binding nucleic acid molecule and use thereof
The present invention provides a novel nucleic acid molecule that can be used for detection of peanuts. The peanut-binding nucleic acid molecule of the present invention is characterized in that it binds to a peanut allergen with a dissociation constant of 10 nm or less, and preferably contains a polynucleotide consisting of any of base sequences of seq id nos: 1 to 15, for example.
Nec Solution Innovators, Ltd.
Purification of triphosphorylated oligonucleotides using capture tags
The present invention relates to a method of preparing triphosphate-modified oligonucleotides using a capture tag. The method allows the synthesis and purification of triphosphate-modified oligonucleotides in high yield and purity suitable for pharmaceutical applications..
Rheinische Friedrich-wilhelms-universitaet Bonn
Ucp1 (thermogenin) - inducing agents for use in the treatment of a disorder of the energy homeostasis
The present invention relates to compositions comprising an agent, like a polynucleotide, which induces or upregulates expression of ucp1 for use in treating or preventing a disorder of the energy homeostasis, overweight, adiposity, obesity, metabolic syndrome or related diseases or disorders in a subject. The present invention also relates to a method of treating or preventing a disorder of the energy homeostasis, overweight, adiposity, obesity, metabolic syndrome or related diseases or disorders in a subject comprising administrating a composition comprising a polynucleotide which induces or upregulates expression of ucp1..
L'université Nice Sophia Antipolis
Polypeptides having protease activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having protease activity, and polynucleotides encoding the polypeptides. The invention further relates to the use of such polypeptides in detergent and/or in cleaning processes.
Permanent gene correction by means of nucleotide-modified messenger rna
The present invention relates to a nucleotide-modified messenger rna for the permanent correction of a genetic alteration on a dna. The invention further relates to a nucleotide-modified messenger rna in combination with a repair template.
Eberhard Karls Universitat Tubingen Medizinische F Akultat
Nucleic acid molecules for increased protein production
The present invention relates to the improved production of proteins, preferably enzymes such as lipases. In particular, the invention relates to a mutated signal peptide and nucleotide sequence encoding said signal peptide that results in an increased protein secretion.
Antibody therapeutics that bind cd38
There is disclosed compositions and methods relating to or derived from anti-cd38 antibodies. More specifically, there is disclosed fully human antibodies that bind cd38, cd38-antibody binding fragments and derivatives of such antibodies, and cd38-binding polypeptides comprising such fragments.
Sorrento Therapeutics, Inc.
Antibody therapeutics that bind cd123
There is disclosed compositions and methods relating to or derived from anti-cd123 antibodies. More specifically, there is disclosed fully human antibodies that bind cd123, cd123-antibody binding fragments and derivatives of such antibodies, and cd123-binding polypeptides comprising such fragments.
Sorrento Therapeutics, Inc.
Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..