This page is updated frequently with new Nucleotide-related patent applications.
|| List of recent Nucleotide-related patents
| Methods for performing antisense oligonucleotide-mediated exon skipping in the retina of a subject in need thereof|
A method, an apparatus, and a computer program product for wireless communication are provided. The apparatus determines an observed bit rate based on uplink transmissions of the ue, estimates an available link capacity for the ue, selects an estimate factor, and estimates available uplink throughput for future uplink transmissions of the ue as a function of the observed bit rate, the estimated available link capacity, and the estimate factor..
Assistance Publique-hopitaux De Paris (aphp)
| Dna-conjugated antibodies for improved antibody affinity and reduced antibody cross reactivity|
Disclosed are specific binding molecules (e.g. Antibodies) that are provided in a set of conjugates, where each conjugate comprises an antibody linked to an oligonucleotide (oligo), such as a dna oligonucleotide.
The Board Of Trustees Of The Leland Stanford Junior University
| Microbial markers and uses therefor|
Disclosed are methods for identifying and/or classifying microbes using one or more single nucleotide polymorphisms (snps) in 16s ribosomal rna (16s rrna) of prokaryotes and/or one or more snps in 5.8s ribosomal rna (5.8s rrna) of eukaryotes. Also disclosed are probes, primers and kits that are useful in those methods.
Immunexpress Pty Ltd
| Strategies for high throughput identification and detection of polymorphisms|
The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.. .
| Methods of detecting synthetic urine and matching a urine sample to a subject|
Provided herein are methods for determining if a urine sample comprises synthetic urine, methods for matching a urine sample to a subject, and methods for amplifying dna. Also provided are kits that include a set of at least 3 pairs of a pre-amplification forward and reverse primer, where each pair of pre-amplification forward and reverse primers is designed to amplify 250 to 300 nucleotides of genomic dna that contains one of at least 3 snps, where the pre-amplification forward and reverse primers in each of the three or more pairs of pre-amplification primers contains (i) a sequence of about 17 to about 25 contiguous nucleotides that is complementary to a sequence in the genomic dna and (i) a tag sequence of about 17 to about 25 contiguous nucleotides that is not complementary to a sequence in the genomic dna..
| Oligonucleotide functionalized quantum dots|
The present invention provides a dna-functionalized conjugate comprising single-stranded dna (ssdna) strands conjugated to a semi-conductor nanoparticle, wherein the nanoparticle provides fluorescent emissions. The present invention also provides a method to efficiently conjugate dna strands to a wide variety of quantum dots having fluorescent emissions.
Arizona Board Of Regents On Behalf Of Arizona State University
| Detecting single nucleotide polymorphism using overlapping hydrolysis probes|
Methods for the rapid detection of the presence or absence of a snp in a target nucleic acid in a sample are described. The methods can include performing an amplifying step, a hybridizing step utilizing a double stranded probe with two overlapping snp specific hydrolysis probe sequences where one of the probe sequences can include a hairpin structure toward the 3′ end, and a detecting step.
Roche Molecular Systems, Inc.
| Methods for producing secreted polypeptides having biological activity|
The present invention relates to methods for producing a polypeptide having biological activity, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first polynucleotide encoding the polypeptide operably linked to a second polynucleotide encoding a variant signal peptide or a variant prepropeptide; and (b) isolating the secreted polypeptide having biological activity from the cultivation medium.. .
| Synthesis of sialic acid in plants|
A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided.
Universite De Rouen
| Targeted integration|
The present disclosure encompasses an isolated cell comprising an exogenous nucleic acid sequence located within or proximal to a predetermined genomic locus, wherein the exogenous nucleic acid sequence comprises at least one recognition sequence which can be exploited by one or more polynucleotide modification enzymes for targeted integration of a recombinant protein. The disclosure further provides methods for preparing such cells, and methods for retargeting such cells for the production of recombinant proteins, and kits for the same..
Sigma-aldrich Co. Llc
Method for propagating sterile male plant line
A method for propagating a sterile male plant line includes: (a) providing a first plant that comprises a homozygous recessive male sterility allele; (b) providing a second plant comprising the homozygous recessive allele and a construct, where the construct exists in a heterozygous state and comprises i) a first nucleotide sequence that will restore the male fertility of the first plant after expression, and ii) a second nucleotide sequence that is able to affect the grain shape or the endosperm nutrient material composition in a heterozygous state, which allows for distinguishing the grains with or without said construct by observation through naked eyes or devices; the first nucleotide sequence and the second nucleotide sequence are tightly connected with each other and coexist in plants; and (c) fertilizing the female gametes of the first plant with the male gametes of the second plant.. .
China Agricultural University
Root-preferred promoter and methods of use
The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter for the gene encoding sorghum bicolor pltp.
Pioneer Hi-bred International, Inc.
Woody plants having improved growth characteristics and making the same
The present invention pertains to a novel and extensive analytical platform for selecting genes with a possible commercial phenotype from a large group of candidate genes identified using tools in bioinformatics, data from est sequencing and dna array. An aspect of the invention provides a method of producing a transgenic plant having an increased growth compared to its wild type.
Swetree Technologies Ab
Method for the targeted killing of cells by nucleotide molecules that are directed to mrna binding, and also nucleotide molecules and application kit for such use
nucleotide molecules are used for the targeted killing of cells, which bind with a nucleotide sequence to a single region of the mrna which, according to sequencing analyses, is statistically very rarely subject to a mutation and, thus, also in case of increased mutation rates in the whole genome, reliably kills the cell without further mrna binding or other influence on the cell being necessary.. .
Rnai agent for inhibition of chikungunya virus
Provided herein are rnai agents for inhibition of chikungunya virus. The present disclosure provides rnai agents for inhibition of chikungunya virus, particularly by targeting the e2 gene and nsp1 gene or both of the chikungunya virus; the rnai agents comprising of the entire nucleotide sequence set forth is seq id 1 or seq id 5 or combination thereof; or comprising of 15 or more contiguous nucleotides as set forth is seq id 1 or seq id 5 or combination thereof along with the addition nucleotides from the contiguous region of the e2 and nsp1 target gene.
Indian Council Of Medical Research
Multivalent aptamer complexes
A compound of the formula a-b-c, is provided, wherein: a is a first nucleic acid that specifically binds to an extracellular surface protein expressed by a cell of interest, b is an alkyl linker; and c is a second nucleic acid that hybridizes to a complementary nucleic acid. In some embodiments, the first nucleic acid is an aptamer.
Wake Forest University Health Sciences
Double-stranded agents for delivering therapeutic oligonucleotides
Disclosed are double-stranded nucleic acid agents that can deliver a therapeutic oligonucleotide within a biological sample, and methods for using the same. In one embodiment, the double-stranded nucleic acid agent comprises a first strand comprising a first rna region, and a second strand comprising a first dna region, wherein said first rna region and said first dna region are hybridized as a rna/dna heteroduplex.
National University Corporation Tokyo Medical And Dental Unviversity
Oligonucleotide duplexes comprising dna-like and rna-like nucleotides and uses thereof
Novel oligonucleotide pairs which can form a duplex comprising one or more dna-like nucleotides (e.g., 2′-substituted arabinonucleotides (ana)); in combination with one or more rna-like nucleotides (e.g., 2′-substituted ribonucleotides (rna) and/or locked nucleic acid nucleotides (lna)), are disclosed. The use of such oligonucleotide duplexes, such as for silencing the expression of a nucleic acid or gene of interest using small interfering rna (sirna) technologies, is also disclosed..
Paladin Labs Inc.
Nuclease-resistant dna analogues
The present invention provides stable, nuclease-resistant tna and tna-dna oligonucleotides, wherein the oligonucleotides are completely resistant to enzymatic degradation for at least 24-72 hours. Methods of synthesis and use in diagnostic and therapeutic applications are also provided.
An integrated system for library construction, affinity binder screening and expression thereof
A recombinant polynucleotide suitable for use in a display vector is provided. The recombinant polynucleotide includes from 5′ to 3′: a first nucleic acid sequence (or insert) encoding an amino acid sequence to be displayed on a surface; a first pre-selected restriction site; a second nucleic acid sequence encoding a surface peptide capable of being displayed on the surface; and a second pre-selected restriction site.
Compositions for making random codon-mutant libraries and uses thereof
The present disclosure relates to compositions and methods for randomly introducing codon-mutations in a target nucleic acid molecule and, more particularly, using wild-type and triplet-randomized oligonucleotides to introduce mutations uniformly across a target nucleotide of interest in a controlled fashion and with a low rate of insertions or deletions.. .
Fred Hutchinson Cancer Research Center
Subtilase variants and polynucleotides encoding same
The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g.
Subtilase variants and polynucleotides encoding same
The present invention relates to novel subtilase variants exhibiting increased stability and optionally on par or improved wash performance. The variants of the invention are suitable for use in e.g.
Protease variants and polynucleotides encoding same
The present invention relates to protease variants and methods for obtaining protease variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants..
Glucoamylase variants and polynucleotides encoding same
The present invention relates to glucoamylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants..
Aad-1 event das-40278-9, related transgenic corn lines, and event-specific identification thereof
This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell.
Dow Agrosciences Llc
Mutated rep encoding sequences for use in aav production
Nucleic acids encoding parvoviral rep proteins with a mutated nuclear localization signal (nls) are provided. Also provided is a nucleic acid comprising a nucleotide sequence encoding a parvoviral rep protein with a mutated zinc finger domain and a nucleic acid comprising a nucleotide sequence encoding a parvoviral rep protein comprising an amino acid mutation at position 43, 57, 79, 97, 120, 179, 305, 484, 493 or 571 with reference to seq id no: 2.
Uniqure Ip B.v.
Detergent compositions comprising metalloproteases
The present invention relates to the use of and compositions comprising isolated polypeptides having protease activity and polynucleotides encoding the polypeptides. The invention also relates to cleaning compositions, and the use of the polypeptides in cleaning processes..
Bispecific t cell activating antigen binding molecules
The present invention generally relates to novel bispecific antigen binding molecules for t cell activation and re-direction to specific target cells. In addition, the present invention relates to polynucleotides encoding such bispecific antigen binding molecules, and vectors and host cells comprising such polynucleotides.
Roche Glycart Ag
Human antigen binding proteins that bind beta-klotho, fgf receptors and complexes thereof
The present invention provides compositions and methods relating to or derived from antigen binding proteins activate fgf21-mediated signaling. In embodiments, the antigen binding proteins specifically bind to (i) β-klotho; (ii) fgfr1c, fgfr2c, fgfr3c or fgfr4; or (iii) a complex comprising β-klotho and one of fgfr1c, fgfr2c, fgfr3c, and fgfr4.
Antibodies against glucocorticoid-induced tumor necrosis factor receptor (gitr) and uses thereof
Provided herein are antibodies, or antigen binding portions thereof, that bind to glucocorticoid-inducible tnf receptor (gitr). Also provided are uses of these proteins in therapeutic applications, such as in the treatment of cancer.
Bristol-myers Squibb Company
Cd19 specific chimeric antigen receptor and uses thereof
The present invention relates to chimeric antigen receptors (car). Cars are able to redirect immune cell specificity and reactivity toward a selected target exploiting the ligand-binding domain properties.
Novel polypeptide, and use thereof
This invention provides a novel means that allows efficient secretion and production of a target protein in a host cell. This invention concerns a novel polypeptide having an activity of improving the secretion productivity of a target protein, a gene comprising a nucleotide sequence encoding such polypeptide, a vector comprising such gene, a transformant obtained by transforming a host cell with such vector, and a method for producing a protein comprising a step of culturing such transformant and recovering a target protein from the culture product..
National Institute Of Advanced Industrial Science And Technology
Oligonucleotides containing high concentrations of guanine monomers
This invention pertains to methods for oligonucleotide synthesis, specifically the synthesis of oligonucleotides that contain a high content of guanine monomers. In more detail, the invention relates to a method for coupling a nucleoside phosphoramidite during the synthesis of an oligonucleotide to a universal support, to a first nucleoside, or to an extending oligonucleotide.
Cytos Biotechnology Ag
Phosphoroamidate esters, and use and synthesis thereof
Phosphoramidate esters and related nucleotide analogs useful in polynucleotide sequencing techniques, and synthetic methods for preparing those compounds, are disclosed. These compounds include nucleotide phosphoramidates analogs that are modified on the alpha-phosphate to enable attachment of a variety of application-specific substituents such as tether molecules..
Stratos Genomics, Inc.
Assembly of high fidelity polynucleotides
Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically, design of support-bound template oligonucleotides is disclosed.
Microfluidic devices and methods for gene synthesis
Certain aspects of the present invention provide devices and methods for preparing oligonucleotides and for assembling nucleic acid molecules using microfluidic devices.. .
Methods for expressing a polynucleotide of interest in the retina of a subject
The present invention relates to methods for expressing a polynucleotide of interest in the retina of a subject. In particular, the present invention relates to a method for expressing a polynucleotide of interest in the retina of a subject comprising the step consisting of injecting into the vitreous an amount of a vector containing the polynucleotide of interest in combination with an amount of an inhibitor of dp71 expression..
Centre National De La Recherche Scientifique (cnrs)
The present disclosure relates to a method for treatment or prevention of diseases have an increased level of insulin-like growth factor i (igf-i). The method comprises administration of a growth hormone (gh) variant having antagonistic activity in combination with an oligonucleotide targeted to growth hormone receptor (ghr) to a subject in need..
Antisense Therapeutics Ltd
A modified coxsackievirus showing improved safety and/or aggressiveness to be used for oncolytic virotherapy is provided. A modified coxsackievirus showing tissue-specific suppression of proliferation and comprising a imitated genome consisting of the genome of coxsackievirus b3 wild-type (cvb3-wt) inserted with at least one polynucleotide consisting of a target sequence of tissue-specific micror na (mirna) is provided.
Kyushu University National University Corporation
Polypeptides having phospholipase c activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having phospholipase c activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
Method for forming personal nutrition complex according to incidence of disease and genetic polymorphism by a prediction system
The present invention relates to a system for predicting an incidence of disease from genetic polymorphism and uses the prediction result to form a personal nutrition complex. The system collects at least one personal information and single nucleotide polymorphism (snp) information then exchanges the above information with databases including a personal database, a genetic risk database, an allelic frequency database, and a prevalence database.
Tci Gene, Inc.
Serotonin transporter gene and treatment of alcoholism
The gene responsible for encoding sert has a functional polymorphism at the 5′-regulatory promoter region, which results in two forms, long (l) and short (s). The ll-genotype is hypothesized to play a key role in the early onset of alcohol use.
University Of Virginia Patent Foundation
Design, synthesis and use of synthetic nucleotides comprising charge mass tags
Embodiments of the present disclosure relate generally to reporter compositions which are synthetic nucleotides that comprise nucleotides with a high charge mass moiety attached thereto via a linker molecule. The linker molecules can vary in length in part to enable the high charge mass moiety to extend out from a dna polymerase complex so that polymerization may not be influenced..
Quantumdx Group Limited
Complex sets of mirnas as non-invasive biomarkers for colon cancer
Described herein are non-invasive methods, kits and means for diagnosing and/or prognosing of colon cancer a body fluid sample from a subject. Further described herein are sets of polynucleotides or sets of primer pairs for detecting sets of mirnas for diagnosing and/or prognosing of colon cancer in a body fluid sample from a subject.
Comprehensive Biomarker Center Gmbh
Method and device for identifying nucleotide, and for determining nucleotide sequence of polynucleotide
The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value.
Method of determining the nucleotide sequence of oligonucleotides and dna molecules
The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection.
Life Technologies Corporation
Charge perturbation detection system for dna and other molecules
Methods and apparatus for direct detection of chemical reactions are provided. Electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule.
The Board Of Trustees Of The Leland Stanford Junior University
Photoblocked probes and methods for sequential detection of nucleic acids
Photoblocked probes are disclosed including a specific hydrolysis probe having a first nucleic acid sequence complementary to a target having a second nucleic acid sequence, a first and a second interactive labels, a 5′ end and a 3′ end, and one or more photocleavable moieties coupled to one or more nucleotides of the specific hydrolysis probe, wherein the photocleaveable moiety interferes with the hybridization of the specific hydrolysis probe with the region of the amplification product. Also disclosed are pcr methods for the detection of the presence or absence of a target nucleic acid in a sample utilizing the photoblocked probes, as well as kits..
Roche Molecular Systems, Inc.
Improved polynucleotide sequences encoding tale repeats
The present invention is in the field of the gene editing molecular tools. The present invention relates to rewritten nucleic acid sequences encoding repeated dna recognition motifs of tale (transcription activator-like effector) proteins.
Compositions and methods directed to crispr/cas genomic engineering systems
The invention relates to engineered crispr/cas9 systems for genomic modification in mammalian cells. The present specification describes the design and testing of a polynucleotide encoding the streptococcus pyogenes (s.
System Biosciences, Llc
Wheat stem rust resistance gene
The present invention relates to a transgenic plant which has integrated into its genome an exogenous polynucleotide encoding a polypeptide which confers resistance to puccinia graminis f sp. Tritici, such as the ug99 group of races puccinia graminis f.
Commonwealth Scientific And Industrial Research Organisation
Methods of increasing abiotic stress tolerance and/or biomass in plants
Provided are methods of increasing the tolerance of a plant to abiotic stresses and/or increasing the biomass and/or increasing the yield of a plant by expressing within the plant an exogenous polynucleotide encoding a polypeptide homologous to seq id no:240, such as the polynucleotide set forth by seq id no:14.. .
Novel genes involved in biosynthesis
The invention provides a novel myb class transcription factor gene (nucleic acid sequences, protein sequences, and variants and fragments thereof) designated myb14 by the applicants, that is useful for manipulating the production of flavonoids, specifically condensed tannins, in plants. The invention provides the isolated nucleic acid molecules encoding proteins with at least 70% identity to any one of myb14 polypeptide sequences of seq id no: 14 and 46 to 54.
Grasslanz Technology Limited
Protein production in plants
The present disclosure relates to methods and constructs for producing heterologous peptides and proteins in plants in a safe and controlled manner. One aspect of the present invention provides a method of producing heterologous protein in a transformed potato plant using an expression cassette comprising a gene coding for a protein or peptide of interest and a marker gene, a nucleotide sequence capable of suppressing patatin expression, along with a nucleotide sequence capable of suppressing cd4b expression, and/or a nucleotide sequence capable of overexpressing p19.
J.r. Simplot Company
Antisense-oligonucleotides as inhibitors of tgf-r signaling
The present invention relates to antisense-oligonucleotides having a length of at least 10 nucleotides, wherein at least two of the nucleotides are lnas, their use as inhibitors of tgf-r signaling, pharmaceutical compositions containing such antisense-oligonucleotides and the use for prophylaxis and treatment of neurological, neurodegenerative, fibrotic and hyperproliferative diseases.. .
Neurovision Pharma Gmbh
The present invention relates to oligomeric compounds and conjugates thereof that target proprotein convertase subtilisin/kexin type 9 (pcsk9) pcsk9 mrna in a cell, leading to reduced expression of pcsk9. Reduction of pcsk9 expression is beneficial for a range of medical disorders, such as hypercholesterolemia and related disorders..
Roche Innovation Center Copenhagen A/s
Treatment of brain derived neurotrophic factor (bdnf) related diseases by inhibition of natural antisense transcript to bdnf
The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of brain derived neurotrophic factor (bdnp), in particular, by targeting natural antisense polynucleotides of brain derived neurotrophic factor (bdnf). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of bdnf..
Immune regulatory oligonucleotide (iro) compounds to modulate toll-like receptor based immune response
The invention provides novel immune regulatory oligonucleotides (iro) as antagonist of tlrs and methods of use thereof. These iros have unique sequences that inhibit or suppress tlr-mediated signaling in response to a tlr ligand or tlr agonist.
Idera Pharmaceuticals, Inc.
Anti-mir-27b and anti-mir-148a oligonucleotides as therapeutic tools for treating dyslipidemias and cardiovascular diseases
The present invention relates to anti-mir-27b and anti-mir-148a oligonucleotides that are capable of decreasing the level and/or activity of mir-27b and mir-148a, respectively. In conjunction with the oligonucleotide molecules of the present invention, the invention also provides a method for decreasing the level and/or activity of mir-27b and/or mir-148a in a cell.
New York University
Antisense oligonuclteotides for treatment of cancer stem cells
The invention provides oligonucleotides complementary to a non-coding chimeric mitochondrial rna as well as compositions and kits comprising the same, and their use in treating and preventing metastasis or relapse of a cancer in an individual previously treated for cancer with a therapy. The invention also provides oligonucleotides complementary to a non-coding chimeric mitochondrial rna as well as compositions and kits comprising the same, and their use in treating a refractory cancer (e.g., a refractory hpv-associated cancer)..
Andes Biotechnologies S.a.