|| List of recent Nucleotide-related patents
| Hsf-like transcription factor, tbf1, is a major molecular switch for growth-to-defense transition in plants|
The present invention relates to new methods to study and control the expression of plant genes, particularly nucleotide sequences located downstream from regions comprising binding sites for transcription factors, such as the cis-element translocon 1 (tl1) comprising gaagaagaa and similar sequences. The invention relates to isolated nucleotide sequences comprising a regulatory region comprising a promoter operably-linked to one or more upstream open reading frames (uorfs) and one or more downstream open reading frames (dorfs) encoding one or more functional polypeptides, including transcription factors such as tbf1, reporter polypeptides, and polypeptides conferring resistance to drugs, resistance of plants viral, bacterial, or fungal pathogens, and polypeptides involved in the growth of plants.
| Methods and compositions for root knot nematode control|
The present invention discloses gene targets, constructs and methods for the genetic control of plant disease caused by nematodes of the genus meloidogyne (root knot nematodes). The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant dna technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences in the cells of plant-parasitic nematodes.
Monsanto Technology Llc
| Polynucleotides encoding rodent antibodies with human idiotypes and animals comprising same|
The invention relates to polynucleotides, particularly chimeric polynucleotides useful for optimal production of functional immunoglobulins with human idiotypes in rodents. The invention further relates to rodents comprising such polynucleotides..
| Oligonucleotide synthesis method using highly dispersible liquid-phase support|
Wherein a condensation reaction is performed by preliminarily dissolving the hydrophobic group-bonded nucleoside or hydrophobic group-bonded oligonucleotide and the nucleoside phosphoramidite compound in a non-polar solvent, and contacting the resulting solution with the acid/azole complex compound or a solution containing the complex compound.. .
| Construction of cell penetrating nucleic acids|
This invention provides ph-responsive zwitterionic nucleotides and nucleic acids comprising said nucleotides, wherein said zwitterions are constituted from one or more anionic internucleoside linkages and one or more cationic moieties and said zwitterionic nucleotides further comprise either one or more hydrophobic moieties or one or more tee's with the general structure (i) hydrophobic element-ph-responsive hydrophilic elements (i).. .
| Phosphorodiamidate backbone linkage for oligonucleotides|
This invention relates to antisense oligonucleotides comprising at least one n3′→p5′ phosphorodiamidate linkage (npn) in the backbone as well as methods for using the same. The antisense oligonucleotides can effectively prevent or decrease protein expression..
| Endothelial cell-specific polynucleotides and use thereof|
An isolated polynucleotide is disclosed comprising a nucleic acid sequence encoding a polypeptide having an amino acid sequence of hif-1alpha, the polypeptide being stably expressed and constitutively active. Isolated polypeptides encoded by same are also disclosed and uses thereof..
Vascular Biogenics Ltd.
| Aav vector compositions and methods for gene transfer to cells, organs and tissues|
The invention relates to adeno-associated virus (aav) serotype aav-rh74 and related aav vectors, and aav-rh74 and related aav vector mediated gene transfer methods and uses. In particular, aav-rh74 targets polynucleotides to cells, tissues or organs for expression (transcription) of genes encoding therapeutic proteins and peptides, and polynucleotides that function as or are transcribed into inhibitory nucleic acid sequences..
The Children's Hospital Of Philadelphia
| Methods for the treatment of leber congenital amaurosis|
The present invention relates to a method for treating a leber congenital amaurosis in a patient harbouring the mutation c.2991+1655 a>g in the cep290 gene, comprising the step of administering to said patient at least one antisense oligonucleotide complementary to nucleic acid sequence that is necessary for preventing splicing of the cryptic exon inserted into the mutant c.2291+1655 a>g cep290 mrna. .
Assistance Publique Hopitaux De Paris
| Treatment of cancers with micro-rna inhibitors|
The invention relates to the treatment and prevention of cancers, including blood-based cancers and breast cancers, by administering agents that inhibit the activity of micrornas, including mir-22. Inhibitors can include oligonucleotides that are at least partially complementary to these mirnas.
Beth Israel Deaconess Medical Center
Rna-interference-inducing nucleic acid molecule able to penetrate into cells, and use therefor
The present invention relates to a novel, rnai-inducing nucleic acid molecule having cell penetrating ability and the use thereof, and more particularly, to a novel, rnai-inducing double-stranded nucleic acid molecule, which has a replacement of the phosphate backbone of at least one nucleotide with phosphorothioate or phosphorodithioate, and has a lipophilic compound conjugated thereto, and thus has high target gene-silencing efficiency while having the ability to penetrate cells without needing a separate intracellular delivery vehicle, and to a method of silencing a target gene using the nucleic acid molecule. The nucleic acid structure according to the present invention has both cholesterol modification and phosphorothioate modification introduced therein, and thus has high gene silencing efficiency while having the ability to penetrate cells without needing a separate intracellular delivery vehicle.
Compositions and methods for decreasing leukocyte extravasation and vessel fluid leakage
Provided herein are methods of decreasing leukocyte extravasation from a lymph or blood vessel into a tissue in a mammal, methods of decreasing fluid leakage from a lymph or blood vessel in a mammal in need thereof, methods of decreasing formation of atherosclerotic plaques in a mammal in need thereof, and methods of treating atherosclerosis in a mammal that include administering to the mammal an oligonucleotide that decreases mitogen-activated protein kinase kinase kinase kinase 4 (map4k4) mrna expression in an endothelial cell. Also provided are methods of identifying a candidate agent useful for decreasing leukocyte extravasation or decreasing fluid leakage from a lymph or blood vessel in a mammal, and compositions containing an oligonucleotide that decreases map4k4 mrna expression in an endothelial cell and additional therapeutic agents..
University Of Massachusetts
The invention is a device for testing an individual for the presence of a entity. The entity can be a pathogen, toxin or drug.
Methods for adding adapters to nucleic acids and compositions for practicing the same
Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (rna), a template switch oligonucleotide including a 3′ hybridization domain and a sequencing platform adapter construct, a polymerase, and dntps.
Clontech Laboratories, Inc.
Multiplex isolation of protein-associated nucleic acids
The invention provides novel methods and materials for genetic and genomic analysis using single or multiplex isolation of protein-associated nucleic acids, including transposase-assisted chromatin immunoprecipitation (tam-chip) and antibody-oligonucleotide proximity ligation. These methods comprise tagging and isolating chromatin or other protein-associated nucleic acids and using antibody-oligonucleotide complexes that recognize the proteins associated with such nucleic acids..
Nucleic acid-based authentication codes
This invention relates to a nucleic-acid based product authentication by determining authentication codes comprising target nucleic acids using oligonucleotide probes immobilized on particulate and non-particulate substrates. The presence of the authentication code is determined using detection methods, such as flow cytometric methods, capable of particle discrimination based on the light scattering or fluorescence properties of the particle, or by spatial resolution of oligonucleotides immobilized at specific loci on a substrate.
Authentiform Technologies, Llc
Method for detecting target nucleic acid
The disclosure of this description includes: a step of preparing a solid-phase body provided with a detection probe having a predetermined nucleotide sequence; a step of preparing a signaling target nucleic acid having a signal generating part for detecting the target nucleic acid; a step of bringing the signaling target nucleic acid, the detection probe, and an oligonucleotide being a mediator having a first site that hybridizes specifically with the target nucleic acid and a second site (preferably 20 to 50 nucleotides or more preferably 20 to 25 nucleotides in length) that hybridizes specifically with a part having the predetermined nucleotide sequence of the detection probe into contact with one another so that a hybridization product of these components can be formed; and a step of obtaining data based on the signal generating part of the signaling target nucleic acid on the solid-phase body.. .
Ngk Insulators, Ltd.
Method for assessing endometrial cancer susceptibility
The present invention aims to provide an assessment method of corpus uteri cancer susceptibility, in which a human chromosomal region consisting of a nucleotide sequence of approximately 100 bps that exhibits a dna copy number polymorphism associated with corpus uteri cancer susceptibility is identified and assessment is made based on an increase or decrease in the dna copy number polymorphism. Corpus uteri cancer susceptibility can be assessed by: first, isolating a chromosomal region exhibiting the dna copy number polymorphism specific for corpus uteri cancer by screening using a microarray derived from the peripheral blood, and subsequently, identifying dna in a human chromosomal region consisting of a nucleotide sequence shown in any of seq id nos: 1 to 7, which is associated with corpus uteri cancer susceptibility, from a vast number of chromosomal regions including not only the chromosomal regions isolated by the above screening, but also regions not covered by the microarray, and then detecting a decrease in dna copy number in the human chromosomal region consisting of the nucleotide sequence shown in any of seq id nos: 1 to 7..
Biocoated piezoelectric biosensor platform for point-of-care diagnostic use
Biosensor components (chips) are described based on direct biocoating processes that result in the tenacious and stable, noncovalent (believed to be chemisorptive) binding of anchor substances such as avidin(s) other proteins having specific binding partners or oligo- or poly-nucleotides onto any piezo-electrically active crystal surface. The resulting platform technology can be developed for a variety of biosensors with specific applications in biological assays.
Aviana Molecular Technologies, Llc.
Method of preparation of nanopore and uses thereof
This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule..
Government Of The United States Of America, As Represented By The Secretary Of Commerce
Hexon isolated from simian adenovirus serotype 19, hypervariable region thereof and chimeric adenovirus using the same
Novel hexon isolated from simian adenovirus serotype 19 encoded in the polynucleotide defined as seq id no: 3, hepervariable region thereof, chimeric adenovirus comprising the same, and therapeutic use thereof provides a solution to the problem of safety and effective systemic treatment for developing gene therapeutic agents using adenovirus.. .
Mogam Biotechnology Research Institute
Enzyme used in biosynthesis of 1, 4-bdo and screening the same
Recombinant butyraldehyde dehydrogenases (blds) with improved production of 1,4-bdo, as well as recombinant microorganisms comprising polynucleotides encoding the recombinant blds, and methods of producing 1,4-bdo by using the recombinant microorganisms.. .
Ajou University Industry-academic Cooperation Foundation
Aldolases, nucleic acids encoding them and methods for making and using them
This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, hmg and/or khg aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, hmg and/or khg aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides.
In vivo production of proteins
The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmrna molecules.. .
Moderna Therapeutics, Inc.
Rad51c as a human cancer susceptibility gene
The invention discloses in vitro methods and a system for determining a predisposition of a subject for developing a cancer on the basis of analyzing a sample of the subject for an alteration of at least one allele of the rad51c gene. Further disclosed are in vitro methods for assessing clinical features or a pathological progression of a cancer and for assessing at least one rad51 c gene alteration in a cell.
Heinrich- Heine-universitÄt DÜsseldorf
Detection of mycoplasma in cell cultures and cell culture derived biologicals
Sequences having specificity for mycoplasma and related mollicutes genus strains and uses thereof. Methods of use include detection of samples contaminated with mycoplasma.
Detection myocobacterium avium spp. paratuberculosis
The invention relates to a method for specifically detecting and optionally for quantifying mycobacterium avium ssp. Paratuberculosis (map) in a sample of an individual.
Georg-august-universitaet Goettingen Stiftung Oeffentlichen Rechts
Novel isolated and purified strains of chikungunya virus and polynucleotides and polypeptides sequences, diagnostic and immunogenical uses thereof
The present invention concerns wild-strains of chikungunya virus isolated from patients exhibiting severe forms of infection and stemming from a human arbovirosis epidemy. The present invention also concerns polypeptide sequences and fragment thereof derived from their genome, the polynucleotide encoding same and their use as diagnostic products, as vaccine and/or as immunogenic compositions..
Methods and compositions for the specific inhibition of gene expression by double-stranded rna
The invention is directed to compositions and methods for selectively reducing the expression of a gene product from a desired target gene in a cell, as well as for treating diseases caused by the expression of the gene. More particularly, the invention is directed to compositions that contain double stranded rna (“dsrna”), and methods for preparing them, that are capable of reducing the expression of target genes in eukaryotic cells.
Integrated Dna Technologies, Inc.
Antimicrobial polynucleotide-templated metal nanocluster
There is provided a polynucleotide-templated metal nanocluster for use in therapy. There is also provided use of a polynucleotide-templated metal nanocluster in the manufacture of a medicament for prophylactically or therapeutically treating a microbial infection as well as a method of prophylactically or therapeutically treating a microbial infection comprising administering to a subject a polynucleotide-templated metal nanocluster.
Agency For Science, Technology And Research
Anti-human cytomegalovirus antibodies and use thereof
This disclosure provides anti-human cytomegalovirus antibodies and methods of treatment, prophylaxis, detection, and diagnosis using the same. In another aspect, the disclosure features therapeutic, prophylactic, and/or diagnostic compositions for human cytomegalovirus infection or for a human cytomegalovirus-related disease that include a binding agent (e.g., antibody) or polynucleotide disclosed herein.
Cell Signaling Technology, Inc.
Methods of impairing osteoclast differentiation using antibodies that bind siglec-15
This invention relates, in part, to unique and newly identified genetic polynucleotides involved in the process of bone remodeling, variants and derivatives of the polynucleotides and corresponding polypeptides, uses of the polynucleotides, polypeptides, variants and derivatives, and methods and compositions for the amelioration of symptoms caused by bone remodeling disorders. Disclosed in particular are the isolation and identification of polynucleotides, polypeptides, variants and derivatives involved in osteoclast activity, validation of the identified polynucleotides for their potential as therapeutic targets and use of the polynucleotides, polypeptides, variants and derivatives for the amelioration of disease states and research purposes..
Alethia Biotherapeutics Inc.
Polypeptides having alpha-glucuronidase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having alpha-glucuronidase activity, catalytic domains and polynucleotides encoding the polypeptides, catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides or catalytic domains..
Modification of cxcr4 using engineered zinc finger proteins
Disclosed herein are methods and compositions for modulating activity of cxcr4 genes, for example using zinc finger transcription factors (zf-tfs) or zinc finger nucleases (zfns) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding zf-tfs or zfns, vectors comprising polynucleotides encoding zf-tfs or zfns and cells comprising polynucleotides encoding zf-tfs or zfns and/or cells comprising zf-tf or zfns are also provided..
Sangamo Biosciences, Inc.
Poly(vinyl ester) polymers for in vivo nucleic acid delivery
The present invention is directed membrane active poly(vinyl ester) polymers and compositions for targeted delivery of rna interference (rnai) polynucleotides to cells in vivo. Rnai polynucleotides are conjugated to the poly(vinyl ester) polymers and the polymers are reversibly modified to enable in vivo targeted delivery.
Arrowhead Madison Inc.
Isolated polynucleotides and polypeptides and methods of using same for increasing plant yield, biomass, growth rate, vigor, oil content, abiotic stress tolerance of plants and nitrogen use efficiency
Provided are isolated polynucleotides comprising a nucleic acid sequence encoding a polypeptide at least 80% identical to seq id no: 422, 362-421, 423-601, 2429-4085 and 4086, such as a polynucleotide which is at least 80% identical to seq id no: 260, 1-259, 261-361, 602-2427 and 2428, nucleic acid constructs comprising same, plant cells comprising same, transgenic plants expressing same, and methods of generating thereof for increasing the yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, nitrogen use efficiency and/or abiotic stress tolerance of a plant.. .
Plant defense genes and proteins and methods of use
Methods and compositions for modulating development and defense responses are provided. nucleotide sequences encoding plant defense proteins are provided.
Pioneer Hi Bred International Inc
Threonine synthase from nicotiana tabacum and methods and uses thereof
There is disclosed a mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a threonine synthase and having at least 90% sequence identity to seq id no:1, seq id no:2 or seq id no:3 or at least 87% sequence identity to seq id no:4, or seq id no:5; (ii) a polypeptide encoded by any one of said polynucleotides set forth in (i); or (iii) a polypeptide having at least 95% sequence identity to seq id no:6, seq id no:7 or seq id no:8; or (iv) a construct, vector or expression vector comprising the polynucleotide as set forth in (i).. .
Philip Morris Products S.a.
Woody plants having improved growth characteristics and making the same using transcription factors
An extensive analytical platform for selecting genes with a possible commercial phenotype from a large group of candidate genes identified using tools in bioinformatics, data from est sequencing and dna array is disclosed. Methods are disclosed for producing a transgenic plant having an increased growth compared to its wild type by altering in the plant the level of a gene product of at least one gene specifically expressed during different phases of wood formation.
Swetree Technologies Ab