|| List of recent Nucleotide-related patents
| Method for detecting a minority genotype|
Disclosed are methods for detecting a minority genotype of a target nucleic acid. The disclosed method generally includes the steps of (a) deep sequencing at least a portion of the target nucleic acid; (b) using the deep sequencing results of (a) to detect the presence of variant nucleobases at one or more nucleotide reference positions within the target nucleic acid; (c) using the variant detection results generated in step (b) to perform a statistical analysis of whether the variants are significant; and (d) using the variant detection and variant significance results generated in steps (b) and (c) to perform a statistical analysis of whether a subset of sequences together exhibit a common set of significant variants..
| Polynucleotide primers|
A polynucleotide primer comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: seq. Id nos.
Qiagen Manchester Limited
| Mir-18b for use as a marker of cancer progression and target for therapies to treat cancer|
The disclosure provides methods for predicting and/or determining whether a subject has cancer based on the level of expression of mir-18b. The disclosure also provides methods for determining whether a cancer in a subject is progressing or regressing based upon the change of expression levels of mir-18b between two time points.
Sutter West Bay Hospitals
| Methods for determining a nucleotide sequence|
Aspects of the technology disclosed herein relate to methods for preparing and analyzing nucleic acids. In some embodiments, methods for preparing nucleic acids for sequence analysis (e.g., using next-generation sequencing) are provided herein..
The General Hospital Corporation
| Photocoupling method using probe containing photoresponsive nucleic acids|
The photocoupling method is characterized by hybridizing a target site present in a nucleic acid sample with a first probe having a sequence complementary to the target site and containing a photo-responsive nucleotide, in a reaction solution, and carrying out photocoupling by photo-irradiation, wherein self-assembly caused by the photo-responsive nucleotide contained in the first probe is suppressed. The photocoupling kit is characterized by comprising a first probe having a sequence complementary to a target site present in a nucleic acid sample, and containing a photo-responsive nucleotide; and a second probe being highly complementary to the first probe..
| Methods and compositions for detection of small rnas|
Currently, the circularization of small rnas is broadly regarded as an obstacle in ligation-related assays and explicitly avoided while short lengths of linear rna targets is broadly recognized as a factor limiting use of conventional primers in pcr-related assays. In contrast, the disclosed invention capitalizes on circularization of small rna targets or their conjugates with oligonucleotide adapters.
| Methods of production of products of metabolic pathways|
Wherein the first, second and third regulatory sequence are selected such that the expression of the icy-b and the crtw is greater than a level of expression of the crti. Methods of generating astaxanthin using the plurality of polynucleotide are also disclosed as well as bacterial cells comprising high levels of astaxanthin..
| Enzymes and polymerases for the synthesis of rna|
The invention relates to compositions and methods for the design, evolution, preparation, and/or manufacture of enzymes for use with polynucleotides, primary transcripts and mmrna molecules.. .
Moderna Therapeutics, Inc.
| Polypeptides for use in the deconstruction of cellulose|
Hydrolysis and degradation of cellulose-containing biomass by use of a polypeptide having cellulase activity is provided. Also provided are polypeptides having cellulase activity, such as archaeal cellulases, polynucleotides encoding the polypeptides, and compositions containing the polypeptides, and methods of use thereof..
The Regents Of The University Of California
| Genes and processes for the production of clavine-type alkaloids|
Microorganisms and processes for the recombinant manufacture of clavine-type alkaloids such as cycloclavine, festuclavine, agroclavine, chanoclavine and chanoclavine aldehyde, as well as polypeptides, polynucleotides and vectors comprising such polynucleotides which can be applied in a method for the manufacture of clavine-type alkaloids are provided.. .
Isolation and characterization of a novel pythium omega 3 desaturase with specificity to all omega 6 fatty acids longer than 18 carbon chains
The present invention relates to a polynucleotide encoding an omega 3 (ω-3) desaturase from pythium irregulare with specificity to long chain polyunsaturated omega 6 (ω-6) fatty acids as well as a vector containing the polynucleotide, and a host cell containing the vector or the polynucleotide. Moreover, the present invention pertains to a polypeptide encoded by the polynucleotide, antibodies against the polypeptide as well as a method for the manufacture of the polypeptide.
Bioriginal Food & Science Corp.
Methods for production of a terpene and a co-product
The present disclosure generally relates to microorganisms (e.g., non-naturally occurring microorganisms) that comprise one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of a carbon source (e.g., a fermentable carbon source) to terpene and a co-product such as succinate, 1,3-butanediol, or crotonyl alcohol and the use of such microorganisms for the production of terpene and a co-product such as succinate, 1,3-butanediol, or crotonyl alcohol.. .
Method of producing a recombinant microorganism
The invention provides a genetic tool to insert, replace, delete, or otherwise manipulate a nucleic acid sequence in a microorganism to produce a recombinant microorganism. Notably, the invention makes use of homologous recombination, a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of dna.
Lanzatech New Zealand Limited
Compositions and methods for increasing pest resistance in plants
Compositions and methods of reducing expression of a flavonoid glucosyltransferase plants, and transgenic and hybrid plants with increased pest resistance are disclosed. The plants can express a polynucleotide that alters, reduces, or silences expression of a flavonoid glucosyltransferase.
University Of Georgia Research Foundation, Inc.
Mycobacterium comprising expression vector with two auxotrophic selection markers and its use as vaccine
The invention relates to polynucleotides and recombinant cell strains comprising the polynucleotides and the uses thereof for the delivery of the polypeptides encoded by the polynucleotides to a subject in need thereof. In particular, the invention refers to polynucleotides comprising a polypeptide of interest, auxotrophy-complementing genes and the use thereof in a mycobacterial double auxotrophic host cell to achieve the stable expression of the polypeptide of interest by using an antibiotic-free plasmid selection system..
FundaciÓ Privada Institut De Recerca De La Sida - Caixa
Antisense oligonucleotides (odn) against smad7 and uses thereof in medical field
The invention relates to antisense oligonucleotidic sequences (odn) against smad7 suitably modified, and their uses in medical field as therapeutic biological agents, in particular in the treatment of chronic inflammatory bowel disease, such as crohn's disease and ulcerative colitis.. .
Nogra Pharma Limited
Restoration of the cftr function by splicing modulation
Oligonucleotides capable of binding to and modulating the splicing of the pre-mrna of the cftr gene, compositions including the oligonucleotides, kits including the compositions, and uses thereof. Compositions of oligonucleotides useful in methods for suppressing exon 10 skipping optionally in combination with additional cftr therapeutics..
The University Of Western Australia
The present invention relates to chirally controlled oligonucleotides, chirally controlled oligonucleotide compositions, and the method of making and using the same. The invention specifically encompasses the identification of the source of certain problems with prior methodologies for preparing chiral oligonucleotides, including problems that prohibit preparation of fully chirally controlled compositions, particularly compositions comprising a plurality of oligonucleotide types.
Wave Life Sciences Pte. Ltd.
Dna-encoded libraries having encoding oligonucleotide linkages not readable by polymerases
The present invention relates to complexes of oligonucleotide-encoded libraries and methods of tagging and using such libraries. In particular, the oligonucleotides and methods can include complexes having at least one linkage for which a polymerase has reduced ability to read or translocate through..
Thermostable asparaginase variants and polynucleotides encoding same
The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Xylanases for solubilising arabinoxylan-containing material
The present invention relates to a method for solubilising arabinoxylan-containing material (particularly insoluble arabinoxylan-containing material), comprising admixing a xylan-containing material with a xylanase comprising a polypeptide sequence shown herein as seq id no. 3, seq id no.
Dupont Nutrition Biosciences Aps
The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds..
Mice are provided that comprise a reduction or deletion of adam6 activity from an endogenous adam6 locus, or that lack an endogenous locus encoding a mouse adam6 protein, wherein the mice comprise a sequence encoding an adam6 or ortholog or homolog or fragment thereof that is functional in a male mouse. In one embodiment, the sequence is an ectopic adam6 sequence or a sequence that confers upon a male mouse the ability to generate offspring by mating.
Regeneron Pharmaceuticals, Inc.
The present invention relates to variants of a parent albumin, the variants having altered plasma half-life compared with the parent albumin. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants..
Novozymes Biopharma Dk A/s
Nucleic acid chemical modifications
Another aspect of the invention relates to a method of inhibiting the expression of a gene in cell, the method comprising (a) contacting an oligonucleotide of the invention with the cell; and (b) maintaining the cell from step (a) for a time sufficient to obtain degradation of the mrna of the target gene.. .
Methods of functionalization and reagents used in such methods using an aza-isatoic anhydride or a derivative thereof, biological molecules thus treated and kits
The present invention relates to a method of functionalization of at least one ribonucleic acid (rna) molecule, contained in a liquid sample, which includes the following steps: a) providing at least: one binding molecule consisting of an aza-isatoic anhydride or a derivative thereof, one group of interest, and one linkage joining the binding molecule to the group of interest, b) reacting the anhydride function of the binding molecule with at least one hydroxyl group carried: in position 2′ of the ribose of one of the rna nucleotides, and/or in position(s) 2′ and/or 3′ of the ribose of the nucleotide at the terminal 3′ end of the rna, and obtaining an aza-anthranilate that joins, by means of the linkage, the rna to the group of interest.. .
Method of preparing porous metal oxide structure
Provided is an example method of preparing a porous metal oxide structure, the method including adsorbing a metal oxide precursor onto a template having a networked structure of branched polynucleotides, decomposing and converting the adsorbed metal oxide precursor into a metal oxide, and removing the template. The networked structure of branched polynucleotides may be used as a template so as to facilitate control of the pore structure of a porous metal oxide structure..
Postech Academy-industry Foundation
Anandamide-modified nucleic acid molecules
The present invention refers to a conjugate comprising at least one polyunsaturated fatty acid residue, particularly an arachidonic acid residue, more particularly an anandamide (arachidonoyl ethanol amide) residue and covalently bound thereto at least one nucleosidic component selected from nucleic acids, nucleosides and nucleotides. This conjugate is suitable for the transfection of cells such as mammalian cells including human cells with high efficacy.
Recombinant modified vaccinia virus ankara (mva) respiratory syncytial virus (rsv) vaccine
Provided herein are recombinant modified vaccinia virus ankara (mva) strains as improved vaccines against infection with respiratory syncytial virus (rsv virus) and to related products, methods and uses. Specifically, provided herein are genetically engineered recombinant mva vectors comprising at least one nucleotide sequence encoding an antigenic determinant of an rsv membrane glycoprotein and at least one nucleotide sequence encoding an antigenic determinant of an rsv nucleocapsid protein.
Bavarian Nordic A/s
Novel prime-boosting regimens involving immunogenic polypeptides encoded by polynucleotides
The present invention relates to administration regimens which are particularly suited for vaccine composition comprising polynucleotides which encode immunogenic polypeptides. Said administration regimens involve, the repeated administration of a vaccine composition and enhance the immune response against the immunogenic polypeptide..
Modified beta-lactamases and methods and uses related thereto
Still further, the invention relates to a polynucleotide and a host cell comprising the polynucleotide.. .
Isolated nucleotide molecule and sensing and killing of pathogenic microorganism
The present invention relates to an isolated nucleic acid molecule comprising (a) a first nucleotide sequence encoding a protein that detects the presence, amount or both of a pathogenic microorganism by forming a complex with a quorum sensing molecule produced by said pathogenic microorganism, (b) one or more second nucleotide sequence said one or more second nucleotide sequence being under control of a promoter that is induced by the complex of the protein encoded by the first nucleotide sequence and the quorum sensing molecule produced by said pathogenic microorganism and encoding (i) an antimicrobial peptide, wherein the antimicrobial peptide is effective against the pathogenic microorganism detected by the protein encoded by the first nucleotide sequence; and/or an antibiofilm enzyme wherein the antibiofilm enzyme is effective against the pathogenic microorganism detected by the protein encoded by the first nucleotide sequence; and (c) optionally a third nucleotide sequence encoding a protein that controls the motility of the host organism, wherein the protein that controls the motility of the host organism directs the motility of the host organism towards said pathogenic microorganism. A recombinant microorganism comprising the isolated nucleic acid molecule and a method of sensing and killing pathogenic microorganisms is also described..
Nanyang Technological University
On01910.na enhances chemotherapeutic agent activity in drug-resistant tumors
The invention includes compositions and methods of treatment of cancers susceptible to treatment with nucleotide analog chemotherapeutic agent, including cancers in which nucleotide analog resistant tumors have developed, including identifying a subject having cancer susceptible to treatment with a nucleotide analog chemotherapeutic agent and a mitotic disruptor/polo-like kinase (pik) pathway inhibitor to a subject; and monitoring the subject for a reduction of stabilization of at least one sign or symptom of cancer.. .
The Johns Hopkins University
Composition for preventing hair loss or enhancing hair growth comprising cyclophilin a
The present invention relates to a composition for preventing hair loss or enhancing hair growth which comprises a cyclophilin a (cypa) protein, a nucleic acid having a nucleotide sequence encoding the cypa protein, or a cypa protein expression recombinant vector. The composition of the present invention is administered into skin cells to inhibit the il-1 expression of the skin cells, to modulate the activities of erk and c-jun, and to increase the expression of nf-kb p65, thereby promoting the growth and development of hair follicles.
University-industry Cooperation Group Of Kyung Hee University
System and methods for detecting genetic variation
The invention provides methods, apparatuses, and compositions for high-throughput amplification sequencing of specific target sequences in one or more samples. In some aspects, barcode-tagged polynucleotides are sequenced simultaneously and sample sources are identified on the basis of barcode sequences.
The present invention provides an apparatus for analysing the sequence of nucleotides in a nucleic acid sample, said apparatus comprising a substrate and a plurality of nanopores provided therein suitable for the passage of nucleic acid molecules therethrough; at least one sample holding chamber disposed upstream of the inlet of said nanopores, at least one detection window juxtaposed within or downstream of the outlet of each nanopore adapted to detect a property characteristic of one or more detectable elements associated with the nucleic acid as each nucleic acid molecule passes therethrough and a detector adapted to generate a data stream characteristic of the various detection events occurring in the detection window characterised in that the apparatus further comprises a means located within the sample holding chamber adapted to increase the local concentration of the nucleic acid sample adjacent the inlet of the nanopores relative to the bulk concentration thereof.. .
Base4 Innovation Ltd
Detection of methylated dna
The use of ion sensitive field effect transistor (isfet) to detect methylated nucleotides in a dna sample is described. A method of detecting methylated nucleotides in a dna sample may include the steps of treating a sample of dna with a reagent which discriminates between methylated and non-methylated nucleotides to provide treated dna, amplifying the treated dna and optionally sequencing the amplified dna.
Primer set for detecting bovine leukemia virus and use thereof
A forward primer of a primer set in accordance with the present invention for detecting blv is a mixture of (1) a first primer consisting of a polynucleotide including 15 or more successive bases including the 16th c in the base sequence of seq id no: 1 and (2) a second primer consisting of a plurality of kinds of polynucleotides including at least the first to 15th bases in the base sequence of seq id no: 2. Two or more of m, n, y, k, and d which are included in the second primer are each a degenerate base which specifies two or more kinds of bases, and the second primer includes at least 10 kinds of polynucleotides including at least the first to 15th bases in the base sequences of seq id nos: 3 to 12..
Riken Genesis Co., Ltd.
Portable amplifying nucleotides and detecting nucleotide sequences
Portable systems and methods for amplifying nucleotides and for detecting nucleotide sequences in a sample are provided. The portable instruments and methods use rpa techniques for dna amplification and detect sample fluorescence in response to amplification and/or to the presence of specific dna sequences..
Monsanto Technology Llc
Method for sequencing a polynucleotide template
The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which permit the sequential determination of nucleotide sequences in two distinct and separate regions on complementary strands of the double-stranded polynucleotide template. The two regions for sequence determination may or may not be complementary to each other..
Illumina Cambridge Limited
Method for sequencing a polynucelotide template
The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.. .
Illumina Cambridge Limited
Sequencing reactions with lithium for pulse width control
Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having lithium that control the median pulse width for incorporated nucleotides are disclosed. The levels of lithium are used to control pulse width while allowing other sequencing parameters to remain within a desirable range..
Pacific Biosciences Of California, Inc.
Genome capture and sequencing to determine genome-wide copy number variation
Provided herein is a capture library for target enrichment of sequences of interest from a genome dna sample. The capture library comprises a plurality of capture oligos tiling a plurality of capture regions evenly-spaced along a genome.
Florida State University Research Foundation
Methods for adding adapters to nucleic acids and compositions for practicing the same
Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template nucleic acid, a template switch oligonucleotide, a polymerase, and dntps.
Clontech Laboratories, Inc.
Compositions and measuring and calibrating amplification bias in multiplexed pcr reactions
Compositions and methods are described for standardizing the dna amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of dna templates that contains rearranged lymphoid cell dna encoding t cell receptors (tcr) or immunoglobulins (ig). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed n high throughput sequencing of amplification products to quantify unique tcr or ig encoding genomes in a sample.
Adaptive Biotechnologies Corp.
Homopolymer mediated nucleic acid amplification
According to some aspects of the invention, provided herein are methods of amplifying nucleic acids using homopolymer-dedicated ligation. The methods, in some embodiments, comprise adding a first homopolymer of at least 12 nucleotides to each 3′ end of blunt-ended double-stranded nucleic acid containing a target nucleic acid, thereby producing a partially double-stranded nucleic acid..
Polypeptides having oxidoreductase activity and their uses
The invention relates to a polypeptide having oxidoreductase activity which comprises the amino acid sequence set out in seq id no: 3 or an amino acid sequence encoded by the nucleotide sequence of seq id no: 4, or a variant polypeptide thereof having 45% or more sequence identity with the sequence of seq id no: 3. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (fdca) or production of 5-hydroxymethyl-2-furancarboxylic acid (hmf acid)..
Purac Biochem B.v.
Recombinant bacteria comprising novel sucrose transporters
Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity.
E.i. Du Pont De Nemours And Company
Compositions and methods for controlling leptinotarsa
Disclosed herein are methods of controlling insect pests, in particular leptinotarsa spp. Which infest crop plants, and methods of providing plants resistant to such pests.
Monsanto Technology Llc
Rnas from pathogens inhibit plant immunity
The present invention relates to pathogen-resistant plants comprising a heterologous expression cassette, the expression cassette comprising a promoter operably linked to a polynucleotide that is complementary to, or mediates destruction, of a plant immunity suppressing srna of a pathogen, wherein the plant is less susceptible to the pathogen compared to a control plant lacking the expression cassette. Methods of making and cultivating pathogen-resistant plants are also provided..
The Regents Of The University Of California
Myb55 promoter and use thereof
The invention provides myb55 promoter sequences that can advantageously be used to express a nucleotide sequence of interest in a plant, plant part or plant cell. Also provided are methods of increasing the expression of a nucleotide sequence of interest in a plant, plant part or plant cell in response to high temperature, abscisic acid, salicylic acid and/or methyl jasmonate.
University Of Guelph
Novel glycosyltransferase gene and use thereof
A polynucleotide is provided that encodes a protein having activity that transfers a sugar to the hydroxyl group at the 4′-position of a flavone. The polynucleotide is selected from the group consisting of: (a) a polynucleotide composed of the base sequence of seq id no: 1; (b) a polynucleotide that hybridizes under stringent conditions with a polynucleotide composed of a base sequence complementary to the base sequence of seq id no: 1, and encodes a protein having activity that transfers a sugar to the hydroxyl group at the 4′-position of a flavone; (c) a polynucleotide encoding a protein composed of the amino acid sequence of seq id no: 2; and, (d) a polynucleotide encoding a protein composed of an amino acid sequence in which one or a plurality of amino acids have been deleted, substituted, inserted and/or added in the amino acid sequence of seq id no: 2, and having activity that transfers a sugar to the hydroxyl group at the 4′-position of a flavone..
Suntory Holdings Limited
Increased protein expression in plants
This disclosure concerns synthetic polynucleotides encoding a polypeptide of interest that are particularly well-suited for expression in target plants.. .
Dow Agrosciences Llc
Methods of treatment using tlr7 and/or tlr9 inhibitors
The application relates to compositions and methods of regulating an immune response comprising inhibitors of tlr7 and/or tlr9, such as immunoregulatory polynucleotides and/or immunoregulatory compounds. The application also relates to compositions and methods for predicting and/or determining responsiveness of a disease to treatment comprising inhibitors of tlr7 and/or tlr9..
Dynavax Technologies Corporation
Oligonucleotide comprising an inosine for treating dmd
The invention provides an oligonucleotide comprising an inosine, and/or a nucleotide containing a base able to form a wobble base pair or a functional equivalent thereof, wherein the oligonucleotide, or a functional equivalent thereof, comprises a sequence which is complementary to at least part of a dystrophin pre-m rna exon or at least part of a non-exon region of a dystrophin pre-m rna said part being a contiguous stretch comprising at least 8 nucleotides. The invention further provides the use of said oligonucleotide for preventing or treating dmd or bmd..
Prosensa Technologies B.v.
Chemical modifications of monomers and oligonucleotides with cycloaddition
In one embodiment, the invention relates compounds and processes for conjugating ligand to oligonucleotide. The invention further relates to methods for treating various disorders and diseases such as viral infections, bacterial infections, parasitic infections, cancers, allergies, autoimmune diseases, immunodeficiencies and immunosuppression..
Double-stranded oligonucleotide molecules to p53 and methods of use thereof
The present application relates to nucleic acid compounds, compositions comprising same and methods of use thereof for treatment of various diseases, disorders and conditions. The compounds are preferably chemically synthesized and modified double-stranded nucleic acid molecules which down regulate expression of a p53 gene..
Quark Pharmaceuticals, Inc.
Novel tetragalnac containing conjugates and methods for delivery of oligonucleotides
Disclosed herein is a modular composition comprising 1) an oligonucleotide; 2) one or more tetragalnac ligands of formula (i), which may be the same or different; optionally, 3) one or more linkers, which may be the same or different; and optionally, 4) one or more targeting ligands, solubilizing agents, pharmacokinetics enhancing agents, lipids, and/or masking agents.. .
Sirna Therapeutics, Inc.
Co-transcriptional assembly of modified rna nanoparticles
A method is provided for generating rna nanoparticles having modified nucleotides and/or having increased nuclease resistance where the rna nanoparticles are formed cotranscriptionally by t7 rna polymerase in the presence of manganese ions.. .
The United States Of America, As Represented By The Secretary, Department Of Health & Human Servic
Template directed split and mix synthesis of small molecule libraries
The invention combines the advantages of split and mix synthesis with the advantages of template directed synthesis. The method comprises the steps of a) adding a linker molecule l to one or more reaction wells; b) adding a molecule fragment to each of said reaction wells; c) adding an oligonucleotide identifier to each of said reaction wells; d) subjecting said wells to conditions sufficient to allow said molecule fragments and said oligonucleotide identifiers to become attached to said linker molecule, or conditions sufficient for said molecule fragments to bind to other molecule fragments and sufficient for said oligonucleotide identifiers to bind to other oligonucleotide identifiers; e) combining the contents of said one or more reaction wells; and f) contacting the resulting bifunctional molecule(s) of step e) with one or more (oligonucleotide) templates each capable of hybridizing to at least one of the oligonucleotide identifiers added step c)..
Compositions and methods for high fidelity assembly of nucleic acids
Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid.
Aspergillus containing beta-glucosidase, beta-glucosidases and nucleic acids encoding the same
A novel microorganism is provided named aspergillus saccharolyticus. Further, beta-glucosidase enzymes encoded by said microorganism are provided, and the use thereof in the degradation of lignocellulosic material.
Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
Provided are isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding modules. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding modules..
Use of pre t alpha or functional variant thereof for expanding tcr alpha deficient t cells
A method of expanding tcralpha deficient t-cells by expressing ptalpha or functional variants thereof into said cells, thereby restoring a functional cd3 complex. This method is particularly useful to enhance the efficiency of immunotherapy using primary t-cells from donors.
Sperm cell separation methods and compositions containing aptamers or nucleic acid sequences for use therein
The present invention provides an aptamer or pool of aptamers (nucleic acid sequences) that bind(s) to a target molecule on the surface, accessible from the surface or inside of a mammalian sperm cell and a method for producing the aptamers. The method comprises contacting a collection of different nucleic acid molecules with the target molecule under conditions favorable for binding between at least one of the nucleic acid molecules and the target molecule, to form at least one complex comprising the nucleic acid molecule bound to the target molecule, wherein each of the nucleic acid molecules comprises at least one segment of randomized nucleotide sequences.
Variants of igg fc with limited amine groups
Lysine-depleted variants of fragment crystallizable (fc) regions of immunoglobulins are disclosed. Also disclosed are fusion proteins comprised of n-terminal targeting or active peptide domains fused with such lysine-depleted variant igg-fc domains.
Long-acting polypeptides and methods of producing same
A polypeptide and polynucleotides encoding same comprising one carboxy-terminal peptide (ctp) of chorionic gonadotrophin attached to an amino terminus of a cytokine and two carboxy-terminal peptides (ctp) of chorionic gonadotrophin attached to a carboxy terminus of a cytokine are disclosed. Pharmaceutical compositions comprising the polypeptide and polynucleotides of the invention and methods of using same are also disclosed..
Opko Biologics Ltd.
Immune system mediator
The present invention discloses an isolated nucleic acid molecule encoding an israa polypeptide comprising a nucleotide sequence exhibiting at least 70% homology to the sequence represented by the nucleotide sequence seq id no: 1. Also disclosed is a recombinant expression vector comprising said nucleic acid molecule and an isolated polypeptide molecule encoded by said nucleic acid molecule..
Arabian Gulf University
New polyphosphate synthesis
The subject of the invention is a new method of the synthesis of polyphosphate analogues, such as nucleosides, oligonucleotides, carbohydrates, peptides and proteins, which are of biological importance and are used in organic chemistry, molecular biology and biotechnology. Polyphosphate analogues, including in particular nucleoside 5′-triphosphates, display high biological activity and are responsible for the provision and storage of energy in live organisms.
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Aminoalcohol lipidoids and uses thereof
Aminoalcohol lipidoids are prepared by reacting an amine with an epoxide-terminated compound are described. Methods of preparing aminoalcohol lipidoids from commercially available starting materials are also provided.
Massachusetts Institute Of Technology