This page is updated frequently with new Nucleotide-related patent applications.
|| List of recent Nucleotide-related patents
| Microrna polymorphisms conferring enhanced drought tolerance in a plant|
Methods of identifying a single nucleotide polymorphism associated with a plant trait and methods of identifying a plant having an improved trait. The plant trait is correlated with at least one single nucleotide polymorphism in a microrna region of a plant genome.
| Dna methylation biomarkers for bladder cancer|
A method for the prediction, prognosis and/or diagnosis of bladder cancer or bladder cancer recurrence in a subject, the method includes: providing a test sample from the subject; measuring dna methylation levels of at least a portion of two or more polynucleotides selected from the group consisting of hoxa9, soxi, npy, irak3, l1-met, and z02 in the test sample; calculating a risk score based on the measured dna methylation levels, comparing the calculated risk score to a cut-off value derived from a reference dna methylation profile based on dna methylation levels of the one or more biomarkers derived from a control group, members of which had bladder cancer; and based on the comparison calculated risk score to the cut-off value, determining at least one of: (1) whether bladder cancer has recurred; (2) whether there is likelihood that the bladder cancer will recur; and (3) whether the patient has bladder cancer.. .
| Biomarkers for the prediction of renal injury|
The present invention relates to means and methods for predicting the onset of renal injury based on measuring the expression of polynucleotides and proteins, particularly on measuring the expression of sets of novel as well as known polynucelotides and proteins, and to kits utilizing same.. .
| Methods for measuring virulence in soybean cyst nematode|
Phytoparasitic nematodes that are able to infect and reproduce on plants that are considered resistant are referred to as virulent. The mechanism(s) that virulent nematodes employ to evade or suppress host plant defenses are not well understood.
| Fus/tls-based compounds and methods for diagnosis, treatment and prevention of amyotrophic lateral sclerosis and related motor neuron diseases|
The invention provides novel fus/tls nucleic acids and proteins that comprise one or more genetic markers (for example, single nucleotide polymorphisms) and methods of use thereof including methods relating to the diagnosis of als or other related motor neuron disease by virtue of the presence of the mutant fus/tls sequence(s).. .
| Polymethine compounds and their use as fluorescent labels|
The present disclosure relates to new compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications..
| Sequencing systems with two slow-step polymerase enzymes|
Systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides..
| Method for simultaneous detection of bacteria and fungi in a biological preparation by pcr, primers as well as bacteria and fungi detection kit|
An exemplary embodiment describes a method for detection of bacteria and fungi in a sample of biological material, wherein the dna contained in the sample of biological material is subjected to amplification in multiplex real-time pcr, with the use of primers specific for bacteria in the first stage and primers specific for fungi, and in the second stage, the resulting dna is amplified using primers and probes differentiating fungi into a group of mold fungi and yeast fungi and bacteria into gram-positive and gram-negative bacteria. Another exemplary embodiment refers to oligonucleotide primers for the detection of bacteria and fungi by pcr and a kit for simultaneous detection of bacteria and fungi..
| Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression|
A method is provided for allele-specific amplification, utilizing a blocking oligonucleotide including at least one nucleotide with a base covalently modified at the exocyclic amino group, the blocking oligonucleotide being perfectly complementary to a wild type (wt) sequence when hybridized forming a first complex having a first melting temperature (tm), the blocking oligonucleotide being partially non-complementary, at one or more nucleotides, to a target mutant (mt) sequence when hybridized forming a second complex having a second melting temperature (tm), wherein the first tm is higher than the second tm and having at least one nucleotide with a base covalently modified at the exocyclic amino group, wherein the blocking oligonucleotide becomes unhybridized from the target mt sequence during amplification but remains hybridized with the wt sequence inhibiting amplification of the wt sequence utilizing a polymerase lacking 5′-3′ nuclease activity.. .
| Microorganisms for diterpene production|
The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-kaurene synthase activity; a polypeptide having ent-kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more udp-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides..
Method for controlled dna fragmentation
A composition and method for controlled in vitro fragmentation of nucleic acids. A transposase forms catalytically active complexes with a modified transposon end that contains within its end sequence degenerate, apurinic/apyrimidinic sites, nicks, or nucleotide gaps, to fragment or shear a target nucleic acid sample in a controlled process.
Polypeptides having glucoamylase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
Expression tools for multiprotein applications
Polynucleotides for multigene applications comprising a novel functional arrangement, as well as vectors, host cells, and recombinant animals comprising the polynucleotides are described. Methods for generating multigene expression cassettes, methods for producing multiprotein complexes in vitro and in vivo, and methods for producing a vaccine are also disclosed.
Pesticidal genes and methods of use
Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, dna constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides.
Axmi281 toxin gene and methods for its use
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided.
Method of inhibiting sprouting in plant tissues
The present invention relates to a method for inhibiting sprouting, particularly pre-harvest sprouting, in plant seeds, by introducing a polynucleotide encoding a fca protein into the plant.. .
Novel protein and gene related to flavonoid o-methyltransferase (fomt) and their uses therefore
The present invention provides novel protein and gene related to flavonoid o-methyltransferase (fomt) and their uses therefore. The said protein having an amino acid sequence shown in seq id no: 3, or an amino acid sequence having deletion, substitution or insertion of one or plural amino acids in said amino acid sequence.
Therapeutic polynucleotides, compositions, and methods
This disclosure describes, in one aspect, a composition that generally includes an xna molecule comprising at least six nucleotides, in an amount effective to improve at least one indicator of myocyte function and a pharmaceutically acceptable carrier. In another aspect, this disclosure describes a method of treating cardiac disease.
Antisense oligonucleotides (odn) against smad7 and uses thereof in medical field
The invention relates to antisense oligonucleotidic sequences (odn) against smad7 suitably modified, and their uses in medical field as therapeutic biological agents, in particular in the treatment of chronic inflammatory bowel disease, such as crohn's disease and ulcerative colitis.. .
Rna with a combination of unmodified and modified nucleotides for protein expression
The invention relates to a polyribonucleotide with a sequence that codes a protein or protein fragment, wherein the polyribonucleotide comprises a combination of unmodified and modified nucleotides, wherein 5 to 50% of the uridine nucleotides and 5 to 50% of the cytidin nucleotides are modified uridine nucleotides or modified cytidin nucleotides.. .
Polypeptides having cellobiohydrolase i activity and polynucleotides encoding same
The present invention relates to polypeptides having cellobiohydrolase i activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides..
Alpha-amylase variants and polynucleotides encoding same
The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants..
Antibodies to tigit
The present invention provides antibodies, or antigen binding fragments thereof, that bind to human tigit (t cell immunoreceptor with ig and itim domains), as well as uses of these antibodies or fragments in therapeutic applications, such as in the treatment of cancer or chronic viral infection. Such method of treatment include combination therapy with inhibitors of other immunomodulatory receptor interactions, such as the pd-1/pd-l1 interaction.
Mps peptides and use thereof
This disclosure provides an isolated polypeptide comprising no more than 35 amino acids, wherein the amino acid sequence comprises, or alternatively consists essentially of, or alternatively consisting of xxxrysyxxsyx (seq id no: 1) and equivalents thereof, wherein x is a basic amino acid and y is a hydrophobic amino acid. Polynucleotides encoding the polypeptides and antibodies that bind to the polypeptides are also provided.
Substituted nucleosides, nucleotides and analogs thereof
Disclosed herein are nucleotide analogs, methods of synthesizing nucleotide analogs and methods of treating diseases and/or conditions such as a hcv infection with one or more nucleotide analogs.. .
Substituted nucleosides, nucleotides and analogs thereof
Disclosed herein are nucleosides, nucleotides and nucleotide analogs, methods of synthesizing the same and methods of treating diseases and/or conditions such as a picornavirus infection with one or more nucleosides, nucleotides and nucleotide analogs.. .
Provided herein are sphingolipid-polyalkylamine phosphoramidites, methods of generating sphingolipid-polyalkylamine-oligonucleotide compounds, pharmaceutical compositions comprising such compounds, and to methods of use thereof in treating cancer.. .
Haemophilus influenzae type iv pili
The invention described herein relates to a haemophilus influenzae (h. Influenzae) regulon encoding type iv pili.
Products and methods for delivery of polynucleotides by adeno-associated virus for lysosomal storage disorders
The present invention relates to methods and materials useful for systemically delivering polynucleotides across the blood brain barrier using adeno-associated virus as a vector. For example, the present invention relates to methods and materials useful for systemically delivering α-n-acetylglucosamidinase polynucleotides to the central and peripheral nervous systems, as well as the somatic system.
Targeting of herpes simplex virus to specific receptors
The invention relates to engineered herpes simplex virus (hsv) particles that are targeted to one or more specific binding pair members, such as receptors. Also, recombinant vectors for producing such hsv particles are provided.
Polypeptides having beta-1,3-galactanase activity and polynucleotides encoding same
The present invention relates to polypeptides having beta-1,3-galactanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
Methods for non-invasive prenatal ploidy calling
Disclosed herein are methods for determining the copy number of a chromosome in a fetus in the context of non-invasive prenatal diagnosis. In an embodiment, the measured genetic data from a sample of genetic material that contains both fetal dna and maternal dna is analyzed, along with the genetic data from the biological parents of the fetus, and the copy number of the chromosome of interest is determined.
Method for determining read error in nucleotide sequence
A method is provided for determining whether a difference between a first reference sequence and first comparative sequences sharing homology with the first reference sequence is caused by a mutation of the first comparative sequence or a read error in sequencing. The method includes generating second comparative sequences and a second reference sequence by substituting for a sequence having the predetermined base number of the consecutive same bases in the first comparative sequences and the first reference sequence, calculating an edit distance of the second comparative sequence to the second reference sequence, and determining whether the difference is caused by the mutation or the read error based on the edit distance..
Nucleic acid sensor for melamine analysis, device for melamine analysis, and melamine analysis
The present invention is to provide a new sensor for melamine detection. The nucleic acid sensor for melamine analysis of the present invention includes a polynucleotide (x1) that includes a catalytic nucleic acid molecule (d) that activates a catalytic function and a binding nucleic acid molecule (a) that binds to melamine.
Nec Solution Innovators, Ltd.
The invention generally relates to nucleotide analogs and methods of their use in sequencing-by-synthesis reactions. In certain embodiments, the invention provides a nucleotide analog including a detectable label attached to a nitrogenous base portion of a nucleotide analog by a cleavable linker, in which contact of the analog with at least one activating agent results in cleavage of the label and elimination of the linker, thereby producing a natural nucleotide, a 9-deaza-g, 9-deaza-a, or ψ-uridine..
Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
A system for determining nucleic acid sequences, including (a) an array of nucleic acid features; (b) a fluidic apparatus configured to deliver sequencing reagents, including polymerases and nucleotides, to the array; (c) a detector configured to obtain pre-equilibrium kinetic measurements from the array at a resolution that distinguishes individual nucleic acid features; (d) a control module having instructions for (i) directing the fluidic apparatus to deliver the sequencing reagents to the array, and (ii) directing the detection apparatus to obtain the kinetic measurements; and (e) an analysis module having instructions for (i) processing the kinetic measurements to determine binding of the polymerase molecules to the nucleic acid features at several pre-equilibrium time points, thereby determining a transient state of the polymerase molecules at the nucleic acid features, and (ii) identifying nucleic acid features that correctly incorporate the nucleotide molecules based on the transient state of the polymerase molecules.. .
Self-assembled single molecule arrays and uses thereof
The present invention provides methods of making and using self-assembled arrays of single polynucleotide molecules for carrying out a variety of large-scale genetic measurements, such as gene expression analysis, gene copy number assessment, and the like. Random arrays used in the invention are “self-assembled” in the sense that they are formed by deposition of polynucleotide molecules onto a surface where they become fixed at random locations.
Callida Genomics, Inc.
Methods and synthesizing nucleic acids
The invention provides improved methods for synthesizing polynucleotides, such as dna and rna, using enzymes and specially designed nucleotide analogs. Using the methods of the invention, specific sequences of polynucleotides can be synthesized de novo, base by base, in an aqueous environment, without the use of a nucleic acid template.
Molecular Assemblies, Inc.
Enhanced cellulose degradation
The present disclosure provides compositions and methods related to the degradation of cellulose and cellulose-containing materials. Cdh-heme domain polypeptides and gh61 polypeptides and related polynucleotides and compositions are provided herein.
The Regents Of The University Of California
3-hydroxypropionic acid production by recombinant yeasts expressing an insect aspartate 1-decarboxylase
Provided herein are recombinant yeast cells having an active 3-hydroxypropionic acid (3-hp) pathway and further comprising a heterologous polynucleotide encoding an aspartate 1-decarboxylase (adc) of the class insecta, bivalvia, branchioporia, gastropoda, or leptocardii. Also described are methods of using the recombinant yeast cells to produce 3-hp and acrylic acid..
Methods and compositions for plant pest control
The present invention comprises methods and compositions for controlling nematode parasitism in host plant. The present invention comprises novel polynucleotides and polypeptides encoded by such polynucleotides comprising one or more nucleic acid sequences disclosed herein having a nucleotide sequence comprising any one of seq id no: 1-142 or 161, a fragment or variant thereof, or a complement thereof, or a polypeptide sequence comprising any one of seq id no: 143-160, a fragment or variant thereof..
The Curators Of The University Of Missouri
Corn plant event mon87460 and compositions and methods for detection thereof
The present invention provides a transgenic corn event mon87460, and cells, seeds, and plants comprising dna diagnostic for the corn event. The invention also provides compositions comprising nucleotide sequences that are diagnostic for mon87460 in a sample, methods for detecting the presence of mon87460 event polynucleotides in a sample, and probes and primers for use in detecting nucleotide sequences that are diagnostic for the presence of mon87460 in a sample.
Monsanto Technology Llc
Clusterin antisense therapy for treatment of cancer
A method for providing antisense therapy which reduces the expression of clusterin to provide therapeutic benefits in the treatment of cancer comprising administering from 40 to 640 mg anti-clusterin antisense oligonucleotide to a patient in need of treatment for a cancer expressing clusterin is provided. The method may include administering chemotherapeutic agent or agents, radiotherapy, and/or hormone ablation therapy.
The University Of British Columbia
A novel class of pharmaceuticals which comprises a locked nucleic acid (lna) which can be used in antisense therapy. These novel oligonucleotides have improved antisense properties.
Roche Innovation Center Copenhagen A/s
A novel class of pharmaceuticals which comprises a locked nucleic acid (lna) which can be used in antisense therapy. These novel oligonucleotides have improved antisense properties.
Roche Innovation Center Copenhagen A/s
Treatment of insulin gene (ins) related diseases by inhibition of natural antisense transcript to an insulin gene (ins)
The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of an insulin gene (ins), in particular, by targeting natural antisense polynucleotides of an insulin gene (ins). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of insulin gene (ins)..
Delivery of therapeutic agent
A method of producing nanovesicles comprising an oligonucleotide inhibitor to an oncogene or a proto-oncogene or the gene product thereof, said method comprises a) introducing a dna sequence encoding an oligonucleotide capable of inhibiting a human oncogenic or proto-oncogenic transcription factor, into a mammalian cell; b) allowing the cell to express said inhibitor oligonucleotide; and c) obtaining nanovesicles containing said inhibitor oligonucleotide from said cell. Nanovesicles produced by the claimed method can be effectively and specifically targeted to e.g.
Cpg-oligodeoxynucleotide, immunogenic composition comprising the same, and methods for preparing the composition and stimulating immune response thereby
Cpg-oligodeoxynucleotides (cpg-odn), as potent immune stimuli developed for using as adjuvant in different species, are disclosed herein. Tlr9 is the cellular receptor for cpg-odn, and current developed cpg-odn has low activity to rabbit tlr9.
National Health Research Institutes
Modulation of exon recognition in pre-mrna by interfering with the secondary rna structure
The invention relates to oligonucleotides for inducing skipping of exon 53 of the dystrophin gene. The invention also relates to methods of inducing exon 53 skipping using the oligonucleotides..
Academisch Ziekenhuis Leiden
Treatment of adiponectin (adipoq) related diseases by inhibition of natural antisense transcript to an adiponectin (adipoq)
The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of an adiponectin (adipoq), in particular, by targeting natural antisense polynucleotides of an adiponectin (adipoq). The invention also relates to the identification of these antisaese oligonucleotides and their use in treating diseases and disorders associated with the expression of adiponectins (adipoq)s..
Una single stranded oligomers for therapeutics
The present invention is directed to rna oligonucleotides or complexes of rna oligonucleotides, denoted herein together as rna complexes, containing at least one, but optionally more that one, hydroxymethyl substituted nucleotide monomer(s). By a hydroxymethyl substituted nucleotide monomer is understood a nucleotide monomer containing a hydroxymethyl group (that may be unsubstituted, o-substituted for example with a conjugating group, or converted into an optionally substituted or conjugated aminomethyl group).
Arcturus Therapeutics, Inc.
Methods and compositions using mir-3151 in the diagnosis and treatment of thyroid cancer
Compositions comprising therapeutic oligonucleotide mir-3151 compounds that target the expression of genes associated with tumorigenesis or cell transformation are provided.. .
The Ohio State University
Modified beta-lactamases and methods and uses related thereto
Still further, the invention relates to a polynucleotide and a host cell comprising the polynucleotide.. .
Cellulases, nucleic acids encoding them and methods for making and using them
This invention relates to molecular and cellular biology and biochemistry. In one aspect, the invention provides polypeptides having cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or β-glucosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides.
Bp Corporation North America Inc.
Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
Polypeptides having lipase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides..
Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.. .
Antibodies directed to her-3 and uses thereof
The present invention relates to binding proteins that bind to her-3 and polynucleotides encoding the same. Expression vectors and host cells comprising the same for the production of the binding protein of the invention are also provided.
Certain improved human bispecific egfrviii antibody engaging molecules
We have constructed a polynucleotide encoding a bispecific antibody engaging molecule which has one arm that specifically engages a tumor cell which expresses the human egfrviii mutant protein on its surface, and a second arm that specifically engages t cell activation ligand cd3. The polynucleotide is codon optimized for expression in cho cells.
The Government Of The U.s. As Represented By The Secretary Of Health
Targeted/immunomodulatory fusion proteins and methods for making same
The present invention relates generally to the field of generating fusion proteins to be used in cancer therapy, and more specifically, to nucleotide sequences encoding the fusion proteins, wherein the chimeric fusion proteins comprises at least one targeting moiety and at least one immunomodulatory moiety that counteracts the immune tolerance of cancer cells.. .
The present invention relates to bi-specific adapters for re-directing viruses to non-virus specific host cells, to expression cassettes comprising a dna molecule having a nucleotide sequence encoding such bi-specific adapters, to recombinant coronaviruses comprising such expression cassettes and to their use as a medicament and their use in the treatment of tumors.. .
Process for production of insulin and insulin analogues
A process for production of insulin or insulin analogues by expression of insulin or insulin analogues through an expression vector in a host cell is provided. The expression vector includes a leader peptide of seq id no 3; a nucleotide sequence encoding an affinity tag linked to c-terminal end or n terminal end of nucleotide sequence of the leader peptide; and a nucleotide sequence encoding for a cleavage site ligated to nucleotide sequence of the leader peptide through nucleotide sequence encoding the affinity tag..
A the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides
Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during dna polymerase-mediated replication of dna or rna polymerase-mediated transcription of rna. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded dna or rna), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker..