|| List of recent Nuclei-related patents
|Compositions, organisms, systems, and methods for expressing a gene product in plants|
The present disclosure relates, in some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant using a expression control sequence (ecs) operable in monocots and/or dicots. For example, (i) an isolated nucleic acid may comprise an ecs (e.g., a sugarcane bacilliform virus promoter) and, optionally, an exogenous nucleic acid (exna) operably linked to the ecs; (ii) an expression vector may comprise an ecs; an exna; and, optionally, a 3′ termination sequence, wherein the ecs has promoter activity sufficient to express the exna in at least one monocot and at least one dicot; (iii) a microorganism, plant cell, or plant may comprise an isolated nucleic acid; (iv) a method for constitutively expressing an exna in a plant (e.g., a monocot and/or a dicot) may comprise, contacting an expression vector with the cytosol of a cell of the plant, wherein the expression vector comprises the exna and an ecs operable to drive expression of the exna; and/or (v) a method of directing constitutive expression of a nucleic acid in a plant (e.g., a monocot and/or a dicot) may comprise transforming the plant with an expression nucleic acid comprising an ecs, an exna, and a 3′ termination sequence..
|Defensin-encoding nucleic acid molecules derived from nicotiana alata, uses therefor and transgenic plants comprising same|
The present invention provides nucleic acid molecules derived from nicotiana alata, which encode defensin-like molecules. The present invention contemplates the use of such nucleic acid molecules in the generation of transgenic plants having resistance or at least reduced sensitivity to plant pests including insects, microorganisms, fungi and/or viruses and the of the encoded defensin-like molecules in compositions for topical application to a plant or a plant part so as to reduce prevent or reduce infestation of the plant or plant part by plant pests.
|Nuclease fusion protein and uses thereof|
The present invention is concerned with nuclease fusion proteins and various uses thereof. Specifically, it relates to a polynucleotide encoding a polypeptide comprising (i) a first module comprising at least a first dna binding domain derived from a homing endonuclease, (ii) a linker, and (iii) a second module comprising at least a second dna binding domain and a cleavage domain derived from a restriction endonuclease, wherein said polypeptide functionally interacts only with dna comprising a dna recognition site for the first dna binding domain and a dna recognition site for the second dna binding domain, and wherein said cleavage domain cleaves dna within a specific dna cleavage site upon binding of the polypeptide.
|Genetics of gender discrimination in date palm|
This invention relates to the genetics of gender discrimination in the dioecious date palm. Methods of the present invention involve analyzing dna or rna from a date palm plant, tissue, germplasm, or seed for the presence of (i) a nucleic acid sequence or genotype that identifies the sex of the plant, tissue, germplasm, or seed or (ii) a molecular marker in linkage disequilibrium with the nucleic acid sequence or genotype.
|Glycoside compound, method for producing thioether, ether, method for producing ether, method for producing glycoside compound, method for producing nucleic acid|
Wherein b, r1, r2, and r3 are as described herein.. .
|Proteins from the webs of nephilengys cruentata, avicularia juruensis and parawixia bistriata spiders isolated from brazilian biodiversity|
The present invention relates to molecules isolated from the nucleic acid that encodes spider web proteins or fragments of these or other derivatives of these. The invention also refers to a chimerical gene and an expression vector containing molecules isolated from the nucleic acid that codes for proteins related to the webs of nephilengys, cruentata, avicularia juruensis and parawixia bistriata spiders.
|Compositions and methods for recognition of rna using triple helical peptide nucleic acids|
Peptide nucleic acids containing thymidine and 2-aminopyridine (m) nucleobases formed stable and sequence selective triple helices with double stranded rna at physiologically relevant conditions. The m-modified pna displayed unique rna selectivity by having two orders of magnitude higher affinity for the double stranded rnas than for the same dna sequences.
|Methods and compositions for diagnosis of alzheimer's disease|
The invention provides a method of diagnosing alzheimer's disease (ad) in a subject by determining the level of expression of one or more mirnas molecules associated with ad, as well as various nucleic acid molecules relating thereto or derived thereof.. .
|Engineered nucleic acids and methods of use thereof for non-human vertebrates|
Provided are formulations, compositions, kits and methods for delivering biological moieties such as modified nucleic acids into cells to induce, reduce or modulate protein expression in non-human vertebrates.. .
|Modulation of apolipoprotein (a) expression|
Compounds, compositions and methods are provided for modulating the expression of apolipoprotein(a). The compositions comprise oligonucleotides, targeted to nucleic acid encoding apolipoprotein(a).
|Compositions and methods for inhibiting expression of factor v|
The invention relates to a double-stranded ribonucleic acid (dsrna) for inhibiting the expression of factor v, comprising an antisense strand having a nucleotide sequence which is less that 25 nucleotides in length and which is substantially complementary to at least a part of factor v. The invention also relates to a pharmaceutical composition comprising the dsrna together with a pharmaceutically acceptable carrier; methods for treating diseases caused by the expression of factor v using the pharmaceutical composition; and methods for inhibiting the expression of factor v in a cell..
|Modulation of growth hormone receptor expression and insulin-like growth factor expression|
Compounds, compositions and methods are provided for modulating the expression of growth hormone receptor and/or insulin like growth factor-i (igf-i). The compositions comprise oligonucleotides, targeted to nucleic acid encoding growth hormone receptor.
|Rotationally sequestered translators|
Provided are nucleic acid translators capable of carrying out logic operations with improved efficiency, maximized output and reduced off-target effects, in particular in a biological system. Methods of using these translators to transduce signal are also provided..
|T cell receptors and related materials and methods of use|
The invention provides t cell receptors (tcrs) having antigenic specificity for a cancer antigen, e.g., tyrosinase. Also provided are related polypeptides, proteins, nucleic acids, recombinant expression vectors, isolated host cells, populations of cells, and pharmaceutical compositions.
|Polypeptides having protease activity and polynucleotides encoding same|
The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Process for determining the concentration of nucleic acids|
A process is disclosed for determining the concentration of nucleic acids in a sample in a microfluidic device. In at least one embodiment, the method includes a) introducing the sample into a first chamber, b) carrying out a number of cycles of an amplification reaction to be carried out in cycles for amplifying nucleic acids, c) transferring a defined volume which is a fraction of the volume of the first chamber and which has amplified nucleic acids into a second chamber and replacing the transferred defined volume with fresh reagents for the amplification reaction, d) determining the concentration of the amplified nucleic acids in a second chamber equipped with an element to determine concentrations, and e) repeating steps b)-d) until a concentration of the amplified nucleic acids which is within a range is determined in the second chamber.
|Method for detecting target nucleic acid|
The disclosure of the present description provides a method for detecting a target nucleic acid, whereby probe hybridization can be accomplished efficiently. To that end, a target nucleic acid is amplified using a first primer having a tag sequence complementary to a detection probe pre-associated with the target nucleic acid and a first recognition sequence that recognizes a first base sequence in the target nucleic acid and also having a linking site capable of inhibiting or arresting a dna polymerase reaction disposed between the tag sequence and the first recognition sequence, and a second primer having a second recognition sequence that recognizes a second base sequence in the target nucleic acid, the amplified fragment is brought into contact with a detection probe so as to allow hybridization, and the hybridization product is detected..
|Fabrication and use of a microfluidics multitemperature flexible reaction device|
Fabrication of a microfluidic multi-temperature reaction device (mmr) and the design and fabrication of the equipment to drive various molecular biological methods on the device are provided. The device can be applicable, for example, to nucleic acid (dna, rna, cdna, etc) amplification, cell lysis, reverse transcription and other enzymatic temperature sensitive and also temperature cycling reactions..
|Nucleic acid construct, nucleic acid-protein complex, and use thereof|
Using a nucleic acid construct, association of a polypeptide with a sequence coding therefor and screening of a polypeptide that binds to a target substance are carried out, which nucleic acid construct comprises a 5′-untranslated region and a coding region, wherein the above-mentioned coding region comprises a sequence coding for a polypeptide subjected to be displayed, a sequence coding for a first nucleic acid binding polypeptide, and a sequence coding for a second nucleic acid binding polypeptide; the above-mentioned 5′-untranslated region comprises a first sequence capable of binding to a first nucleic acid binding polypeptide and a second sequence capable of binding to second nucleic acid binding polypeptide; and, when the above-mentioned nucleic acid construct is introduced in a translation system, a fusion protein translated from the coding region of the above-mentioned nucleic acid construct forms a complex with an rna corresponding to the above-mentioned nucleic acid construct.. .
|Massive parallel method for decoding dna and rna|
This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of dna in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —oh group at the 3′-position of the deoxyribose..
|Step-wise nucleic acid sequencing with catalytic and non-catalytic metals|
The application relates to methods, and systems for nucleotide sequencing comprising producing polymerase reactions that comprise both catalytic and non-catalytic divalent metal ions. Polymerase/template/primer complexes are immobilized on a substrate.
|Microfluidic system for nucleic acid analysis|
A microfluidic system for analyzing nucleic acid, the microfluidic system including a reagent supply device including a sample chamber into which a sample can be injected, one or more reagent chambers for containing one or more reagents for extracting nucleic acid from the sample, and a waste chamber in which the used reagent can be discarded; a binding-lysis chamber in which cells are captured from the sample and lysed to form a cell lysate containing nucleic acid; plurality of particles for cell binding disposed in the binding-lysis chamber; a plurality of rehydration chambers into which the cell lysate formed in the binding-lysis chamber can be distributed and mixed with a nucleic acid amplification reagent to form an amplification reaction mixture; a plurality of amplification chambers in which a nucleic acid amplification reaction is performed on the amplification reaction mixture introduced from the plurality of rehydration chambers; and a flow channel system including an outlet and a plurality of inlets connected to the reagent supply device and forming an integrated fluid flow between the binding-lysis chamber, the rehydration chambers, and the amplification chambers.. .
|Vector with codon-optimised genes for an arabinose metabolic pathway for arabinose conversion in yeast for ethanol production|
The present invention relates to novel expression cassettes and expression vectors, comprising three nucleic acid sequences for araa, arab and arad, each coding for a polypeptide of an l-arabinose metabolic pathway, in particular, a bacterial l-arabinose metabolic pathway. The invention particularly relates to expression cassettes and expression vectors, comprising codon-optimised nucleic acid sequences for araa, arab and arad.
|Transcriptome transfer produces cellular phenotype conversion|
The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell.
|Dna vector production system|
A vector production system is provided. The system comprises recombinant cells designed to encode at least a first recombinase under the control of an inducible promoter and the cells include an expression vector encoding a nucleic acid of interest within the regulatory elements of the expression vector which are flanked on either side by a target sequence for at least the first recombinase.
|Polypeptides having glucoamylase activity and polynucleotides encoding same|
Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains..
|Method for screening alpha-amylases|
The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants..
|Microfluidic device-based nucleic acid purification method|
A method is provided for purifying nucleic acid from a sample in a microfluidic device. The method can be used to purify nucleic acids from any source known in the art that comprises nucleic acids, such as prokaryotic or eukaryotic organisms, viruses, cell, tissues, organs, etc.
|Methods of diagnosing breast cancer|
The disclosed subject matter is based on the discovery that a mutation (a single nucleotide polymorphism or “snp”) in the gene encoding abraxas (also referred to as abra1, ccdc98 or fam175a), is associated with susceptibility to cancer, e.g., breast cancer. In particular, the disclosed subject matter is based on the identification of a heterozygous alteration in the abraxas gene that is associated with breast cancer and specifically correlated with familial cancer.
|Methods and compositions for enrichment of nucleic acids in mixtures of highly homologous sequences|
Provided are methods of enrichment and detection of target nucleic acids during target amplification in the presence of excess amounts of highly homologous sequences, said methods having substantial diagnostic utility (e.g., cancer diagnostics). Provided are amplification reaction mixtures having at least one cleavage-directing oligonucleotide, the respective binding sites of which, for the target and homologous sequences, include one or more nucleotide positions differing in sequence between the target homologous sequences.
|Sequence amplification with linear primers|
The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences..