 Patent Application Title |
Patent App Num. |
Date |
| High throughput screening of ion channels | 20130048511 | 20130228 |
 Multi-well plates having contoured well designs allow multi-stage high throughput parallel assaying of ion channels or ion transporters. A well of a multi-well plate has a bottom region that is sized and shaped to simultaneously accommodate a sensing electrode and a pipette for delivering test compounds, wash fluid, and optionally ligands. The multi-well plates when coupled with an instrument having a pipette head and an electrode plate facilitates fluidic contact between cells and fluids delivered via a pipette for washing of wells with buffers or other wash solutions for serial exposure of test cells to various reagents or other stimuli. The control and test experiments are performed on the same cell (or cells) in a single well.
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| Multi-well plate with filter medium, and use thereof | 20120329163 | 20121227 |
 A multi-well plate (1) has an upper part (2) with a multiplicity of wells (3), a lower part (4) with a multiplicity of wells (5) that communicate with the wells (3) of the upper part (2), and at least one filter medium (6) that can be fixed between the upper and lower parts (2, 4). Sides (8, 9) of the upper and lower parts (2, 4), facing toward the filter medium (6), have seals (7a, 7b) extending around the wells. The filter medium (6) can be fixed along the upper and lower sides in each case by pairs of the seals (7b) of the upper part (2) and the seals (7a) of the lower part (4). The multi-well plate prevents cross-contamination between adjacent wells due to... |
| Methods and devices for crystallization by controlled evaporation | 20120241100 | 20120927 |
 Methods and devices for controlled evaporation of solvent from a solution are provided. In one embodiment, a method for controlled evaporation of solvent from at least one solution includes filling a selected first set of wells in a multi-well plate with a first solution comprising at least one first solvent. The method also includes attaching an inlet manifold to the plate, the inlet manifold comprising an inlet port connected to and in fluid communication only with each of the wells in the selected first set of wells. The method further includes attaching an exhaust manifold to the plate, the exhaust manifold comprising an exhaust port connected to and in fluid communication only with each of the wells in the selected first set of wells. The method... |
| Detection method and device based on nanoparticle aggregation | 20120202218 | 20120809 |
 A method for determining the presence of a compound in a liquid solution, by admixing the liquid solution with a plurality of nanoparticles; providing conditions effective to cause aggregation of the nanoparticles in the liquid solution in the absence of said compound in the liquid solution; and observing a detectable signal reflecting the amount of aggregation of nanoparticles in the liquid solution, wherein the presence of the compound in the liquid solution results in a detectable signal reflecting a reduced amount of aggregation of nanoparticles in the liquid solution, in comparison to the amount of aggregation of nanoparticles obtained in the liquid solution in the absence of the compound therein. A nanoparticle, a composition, a kit and a for multi-well plate for use in the method... |
| Assay apparatuses, methods and reagents | 20120195800 | 20120802 |
 We describe apparatuses, systems, method, reagents, and kits for conducting assays as well as process for their preparation. They are particularly well suited for conducting automated sampling, sample preparation, and analysis in a multi-well plate assay format. For example, they may be used for automated analysis of particulates in air and/or liquid samples derived therefrom in environmental monitoring.
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| Automatic refining apparatus, multi-well plate kit and method for extracting hexane from biological samples | 20110009608 | 20110113 |
| The present invention relates to an automatic refining apparatus for separating target materials from a plurality of biological sample solutions by using magnetic particles to which the magnetic particles are to be reversibly coupled, and to a multi-well plate kit for use in the automatic refining apparatus. Further, the present invention relates to a method for extracting nucleic acids from biological samples by using the above-described automatic refining apparatus. The present invention can be used in the automatic separation of nucleic acid, protein, and the like from biological samples.
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| Sample carrier device incorporating radio frequency identification, and method | 20110001609 | 20110106 |
| An apparatus for associating information with a carrier and/or at least one biological reagent is provided. The apparatus can include a carrier adapted to retain at least one biological reagent and at least one non-silicon Radio Frequency Identification Tag (RFID). The RFID tag can comprise information in the form of an information pointer that can be resolved by an information pointer resolution device. The information pointer and/or resolution device can be used to access a remote system. The carrier can be configured, for example, as a microfluidic processing device or a multi-well plate. Methods and systems using the apparatus are also provided.
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| Porous substrate plates and the use thereof | 20100323915 | 20101223 |
| A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.
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| Gamma-secretase substrates and methods of use | 20100285988 | 20101111 |
| Polypeptide substrates based on modifications or fragments of the various APP isoforms, assay methods based on the use of these substrates, and screening methods directed toward identifying inhibitors of γ-secretase activity. The assay methods and the screening methods are adapted for use in high throughput multi-well plate assay apparatuses. In many embodiments the substrate polypeptides are labeled for ease of detection, and/or may bind specific ligands that themselves are labeled. Generally the labels promote high specificity as well as high sensitivity of detection. These features render the assay and screening methods that employ the labeled substrates especially suited for use in high throughput assay formats. This disclosure further identifies small polypeptides based on a subsequence motif of Aβ that are shown herein to be potent inhibitors... |
| Method of manufacturing a polymer blend | 20100280174 | 20101104 |
| Hydrophobic polymer surfaces whose level of protein binding is less than about 50-80 ng/cm2 are achieved by: (1) applying a coating solution composed of a solvent and a non-ionic surfactant having a HLB number of less than 5 to the surface; and (2) drying the surface to remove the solvent and thereby bring the surfactant into direct contact with the hydrophobic polymer. The combination of a low HLB number and the drying step have been found to produce low binding surfaces which can withstand multiple washes with water and/or protein-containing solutions Alternatively, the low binding surfaces can be produced by applying the non-ionic surfactant to the mold surfaces which contact molten polymer and fault the polymer into a desired shape, e.g., into a multi-well plate, a... |
| Sample carrier system | 20100218623 | 20100902 |
| A sample carrier generally comprises a sample node and an identifier co-located with the sample node. A sample node may be operative to carry a discrete sample, while an identifier may be operative to provide information associated with the sample carried by the sample node. Embodiments of a sample carrier may comprise a plurality of sample nodes supported in a predetermined spatial relationship relative to a respective sample container such as a respective well of a multi-well plate. A system and method of transferring specimens to a sample carrier include contacting a sample node to the specimen and encoding information associated with the specimen on the identifier. Various alternatives are disclosed wherein the specimen is solid, gaseous, and liquid in form.
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| Multi-well plate lid with protective skirt | 20100202927 | 20100812 |
| A plate lid for covering a multi-well plate for use in a high throughput screening (HTS) process. The lid includes a plate section dimensioned to cover the plate, a frame block positioned on the underside perimeter of the plate section to form a recessed center, a skirt section protruding from each edge of the frame block; and a gasket layer positioned on the frame block for sealing the peripheral surface of the multi-well plate.
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| Single-molecule pcr on microparticles in water-in-oil emulsions | 20100190153 | 20100729 |
| Modulation of the viscosity of the oil phase of a microemulsion used for amplification of DNA on a bead increases the homogeneity of product beads and the amount of amplified DNA per bead. Moreover the number of separate microemulsion populations that can be formed in parallel is increased using multi-well plates and mixer mill disruptor machines designed to lyse biological samples.
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| Method and device for analyzing biomolecules with track - etched polymeric layers | 20100137160 | 20100603 |
| The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.
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| Multi-well plate and method of manufacture | 20100119418 | 20100513 |
| A method of manufacture and assembly of multiwell plates employing targeted radiation at an interface in order to achieve bonding is disclosed. The method accommodates glass and polymer attachment as well as polymer to polymer attachment. Resultant plates have unique flatness and optical properties.
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| Localized temperature control for spatial arrays of reaction media | 20100099581 | 20100422 |
| Individual temperature control in multiple reactions performed simultaneously in a spatial array such as a multi-well plate is achieved by thermoelectric modules with individual control, with each module supplying heat to or drawing heat from a single region within the array, the region containing either a single reaction vessel or a group of reaction vessels.
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| Multi-well plate with tailored chambers | 20100064781 | 20100318 |
| Systems, methods and multi-well plates comprising an array of wells for thermal treatment of chemical or biological samples are disclosed. Each of the wells comprises a bottom opening, an upper opening, inner side walls extending from the bottom opening to the upper opening, a protrusion extending from the inner side walls into the well with a first cross-sectional area and located at a height from the bottom opening which is smaller than the height from the upper opening. A sample chamber with a second cross-sectional area is formed between the bottom opening and the protrusion. An upper chamber with a third cross-sectional area is formed between the protrusion and the upper opening, and in which the first cross-sectional area is smaller than the third cross-sectional area... |
| Simultaneous aspirator and dispenser for multiwell plates and similar devices | 20100043571 | 20100225 |
| A device for simultaneously dispensing a solution containing a sample to a test device and aspirating the sample from the device. In one embodiment the device is hand-held and includes a control mechanism, e.g., button for activating the dispensing and aspiration of the sample. The testing device may take the form of a multi-well plate with a plurality of wells arranged in rows and columns, with the bottom of the wells formed as a photonic crystal biosensor. The device can be configured with dispense and aspirate manifolds and associated dispense and aspirate ports which are positioned in all the wells along a row or column of wells so as to simultaneously aspirate and dispense solution to all the wells in a row or column of the... |
| Microplates with ultra-thin walls by two-stage forming | 20100028988 | 20100204 |
| A multi-well plate with wells having ultra-thin walls and yet with sufficient structural rigidity to allow reliable use of the plate in automated instrumentation is formed by first forming a plate blank by injection molding, the blank being of a thickness that provides the desired rigidity, and then subjecting the blank to vacuum forming to stretch designated areas in the blank to form wells or to extend wells already formed, the stretching resulting in a reduction in thickness of the molded resin at the walls of the wells only.
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| Assay apparatuses, methods and reagents | 20090263904 | 20091022 |
| We describe apparatuses, systems, method, reagents, and kits for conducting assays as well as process for their preparation. They are particularly well suited for conducting automated sampling, sample preparation, and analysis in a multi-well plate assay format. For example, they may be used for automated analysis of particulates in air and/or liquid samples derived therefrom in environmental monitoring.
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| Methods for the analysis of dissociation melt curve data | 20090204353 | 20090813 |
| Methods are provided that operate on raw dissociation data and dissociation curves to generate calibrations of the detected data and to further improve analysis of the data. The data can be taken from each support region of a multi-region platform, for example, from each well of a multi-well plate. Each support region can be loaded with portions of the same sample. In some embodiments, a dissociation curve correction can be calibrated for the sample, prior to a run of an experiment using such sample. In some embodiments, a method is provided for generating a melting transition region of dissociation curves that show the melting characteristics of the sample. In some embodiments, dye temperature dependence correction can be performed on the dissociation curve data to further improve... |
| System for automatically loading immunoassay analyzer | 20090117004 | 20090507 |
| A component of a laboratory automation system that integrates (a) separating a solid magnetic substrate from the liquid contents of a reaction vessel, (b) management of the thermal characteristics of the component of the laboratory automation system, (c) automated loading of multi-well plates and tip combs into the component of the laboratory automation system, (d) automated unloading of multi-well plates and tip combs from the component of the laboratory automation system, and (e) reading of radio frequency identification tags attached to multi-well plates.
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| Automated analyzer for clinical laboratory | 20090117620 | 20090507 |
| A laboratory automation system that is capable of carrying out clinical chemistry assays, immunoassays, amplification of nucleic acid assays, and any combination of the foregoing, said laboratory automation system employing at least one of micro-well plates and deep multi-well plates as reaction vessels. The use of micro-well plates as reaction vessels enables the laboratory automation system to assume a variety of arrangements, i.e., the laboratory automation system can comprise a variety of functional modules that can be arranged in various ways. In order to effectively carry out immunoassays by means of micro-well plates, a technique known as inverse magnetic particle processing can be used to transfer the product(s) of immunoassays from one micro-well of a micro-well plate to another.
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| Novel cell-based assays for g-protein-coupled receptor-mediated activities | 20090087906 | 20090402 |
| Disclosed are compositions and methods for their use, such as in identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers.
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| Systems and methods for robotic transport | 20090054222 | 20090226 |
| Systems and methods for robotic transport are disclosed herein. In some embodiments, robotic systems for transporting biological samples include: a plurality of capillary vessels, in which each capillary vessel can contain a biological sample from a population; a receptacle that can contain the plurality of capillary vessels; a centrifuge; a first robotic device that can transport the receptacle between an input module and the centrifuge; a second robotic device that can transport the receptacle between the centrifuge and a sample harvest location; a cutting device that can cut each of the plurality of capillary vessels; a multi-well plate having a plurality of wells arranged in an array; and a third robotic device that can transfer at least one portion of each of the plurality of biological... |
| Mechanical cell wounder device and related method | 20090035844 | 20090205 |
| Disclosed is a device and related method for biological manipulation. With greater particularity, disclosed is a novel device and method for uniform cellular manipulation with economies of scale and operational uniformity. The device and related method utilize a cellular wounder (101) to uniformly remove portions of a cell monolayer in a multi-well plate (303) to create uniform cell samples with reduced time and expense to the user. The multi-channel mechanical wounder is a useful tool for performing cellular wounding by providing consistent and even denudation of a defined area of monolayer of cells in standard culture plates. This easy-to-assemble and user friendly device is most applicable for high throughput screening of samples such as in the manipulation of cells for cell migration or wound healing assay... |
| Methods of screening an agent for an activity in an isolated eye of a teleost | 20090010849 | 20090108 |
| The present invention provides methods of screening an agent for an activity in an isolated organ, e.g., eye, from a teleost, e.g., zebrafish. Methods of isolating eyes from zebrafish are provided. Methods of screening an agent for an ocular activity in the isolated eye are provided. Methods of screening an agent for an ocular activity in a model of ocular disease or disorder are provided. Methods of screening an agent for an ocular activity in the isolated eye and for screening the agent for cell death and/or toxic activity in the eye or other organ or tissue are provided. The invention further provides high throughput methods of screening agents for an activity in isolated eyes of zebrafish in multi-well plates.
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| Scanning spectrophotometer for high throughput fluorescence detection | 20080309933 | 20081218 |
| A fluorescence spectrophotometer having an excitation double monochromator, a coaxial excitation/emission light transfer module, and an emission double monochromator. Each monochromator includes a pair of holographic concave gratings mounted to precisely select a desired band of wavelengths from incoming broadband light without using other optical elements, such as mirrors. Selected excitation light is directed into a sample well by a light transfer module that includes a coaxial excitation mirror positioned to direct excitation light directly to the bottom of a well of a multi-well plate. Fluorescence emission light that exits the well opening is collected by a relatively large coaxial emission mirror. The collected emission light is wavelength selected by the emission double monochromator. Selected emission light is detected by a photodetector module.
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| Scanning spectrophotometer for high throughput fluorescence detection | 20080309934 | 20081218 |
| A fluorescence spectrophotometer having an excitation double monochromator, a coaxial excitation/emission light transfer module, and an emission double monochromator. Each monochromator includes a pair of holographic concave gratings mounted to precisely select a desired band of wavelengths from incoming broadband light without using other optical elements, such as mirrors. Selected excitation light is directed into a sample well by a light transfer module that includes a coaxial excitation mirror positioned to direct excitation light directly to the bottom of a well of a multi-well plate. Fluorescence emission light that exits the well opening is collected by a relatively large coaxial emission mirror. The collected emission light is wavelength selected by the emission double monochromator. Selected emission light is detected by a photodetector module.
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| In vitro root-nematode assay in multi-well plates | 20080311555 | 20081218 |
| The invention provides an improvement in the method of screening roots, both transgenic and non-transgenic, for activity against parasitic nematodes. In particular, the improvement of the invention is use of multi-well cell culture plates for root culture and nematode infection steps.
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| Methods and apparatus for optical analysis of samples in biological sample containers | 20080297774 | 20081204 |
| An apparatus and method for optically analyzing samples in a biological sample container containing samples arranged at different locations on the base of the container. An optical acquisition device is provided comprising a detector and an objective. The position of the upper and lower surfaces of the base at each of the sample locations is determined by a confocal polychromatic displacement sensor. Light is collected from each of the sample locations by adjusting the focal plane to be coincident with, or vertically offset from, the upper surface of the base, as determined from the displacement sensor. This allows for rapid scanning of large numbers of samples in a multi-well plate or other biological sample containers.
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| Methods and apparatus for optical analysis of samples in biological sample containers | 20080297795 | 20081204 |
| An apparatus and method for optically analyzing samples in a biological sample container containing samples arranged at different locations on the base of the container. An optical acquisition device is provided comprising a detector and an objective. The position of the upper and lower surfaces of the base at each of the sample locations is determined by a confocal polychromatic displacement sensor. Light is collected from each of the sample locations by adjusting the focal plane to be coincident with, or vertically offset from, the upper surface of the base, as determined from the displacement sensor. This allows for rapid scanning of large numbers of samples in a multi-well plate or other biological sample containers.
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| Inhibitors of thapsigargin-induced cell death | 20080293699 | 20081127 |
| Methods for screening for inhibitors of endoplasmic reticulum (ER) stress are provided. These methods involve the addition of thapsigargin, which induces ER stress, and a test agent to mammalian cells in multi-well plates. Cell survival can be readily monitored by measuring intracellular ATP content using a bioluminescent reagent. Screening a commercially available library of 50,000 compounds led to the identification of 93 hit compounds that were subjected to secondary assays to confirm their ability to rescue cells from thapsigargin-induced cell death.
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| Sample carrier device incorporating radio frequency identification, and method | 20080238627 | 20081002 |
| An apparatus for associating information with a carrier and/or at least one biological reagent is provided. The apparatus can include a carrier adapted to retain at least one biological reagent and at least one non-silicon Radio Frequency Identification Tag (RFID). The RFID tag can comprise information in the form of an information pointer that can be resolved by an information pointer resolution device. The information pointer and/or resolution device can be used to access a remote system. The carrier can be configured, for example, as a microfluidic processing device or a multi-well plate. Methods and systems using the apparatus are also provided.
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| Planar registration of multi-well plate from well side | 20080199365 | 20080821 |
| A multi-well plate such as a microtiter plate is secured from its well side in a manner that corrects or compensates for any deviations of the plate base from a planar configuration. This is achieved by an apparatus that includes an array of posts with exposed tips that define a plane, a plurality of movable collets that, when appropriately actuated, seize the walls of selected wells, and a biasing means to urge the collets in a direction that forces the wells of the microtiter plate against the exposed tips of the posts.
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| Systems and methods for high-throughput radiation biodosimetry | 20080181473 | 20080731 |
| Systems and methods for high-throughput radiation biodosimetry are disclosed herein. In some embodiments, a high-throughput methods of analyzing a population for radiation exposure can include, in various possible sequences: marking a first capillary designed to retain a first sample from the population with a first identifier; transporting a plurality of samples to a biodosimetry system; inputting the samples into the biodosimetry system; centrifuging the plurality of samples including the first sample wherein each sample can be retained in a capillary and the first sample can be retained in the first capillary; transferring the plurality of capillaries including the first capillary from the centrifuge to a cutting device using a robotic device; cutting the first capillary; reading the first identifier; transferring at least one portion of the... |
| Microcolumn-based, high-throughput microfluidic device | 20080160602 | 20080703 |
| A biological assay device for use in molecular biology, pharmaceutical research, genomic analysis, combinatorial chemistry, and in the general field of the analysis of molecules that may be deposited on supports of various kinds is provided. Specifically, the present invention includes a fluidic or microfluidic device, which integrates fluidic capability into existing multi-well plates of standard configuration, for performing either single or continuous fluidic manipulations in a high-throughout format. Methods for the use and manufacture of these devices are also provided.
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| Methods of screening agents for activity using teleosts | 20080152591 | 20080626 |
| The present invention provides methods of screening an agent for activity using teleosts. Methods of screening an agent for angiogenesis activity, toxic activity and an effect cell death activity in teleosts are provided. Methods of screening an agent for an activity in the brain or central nervous system in zebrafish are provided. The invention further provides high throughput methods of screening agents in multi-well plates.
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| Methods for producing low binding surfaces | 20080145280 | 20080619 |
| Hydrophobic polymer surfaces whose level of protein binding is less than about 50-80 ng/cm2 are achieved by: (1) applying a coating solution composed of a solvent and a non-ionic surfactant having a HLB number of less than 5 to the surface; and (2) drying the surface to remove the solvent and thereby bring the surfactant into direct contact with the hydrophobic polymer. The combination of a low HLB number and the drying step have been found to produce low binding surfaces which can withstand multiple washes with water and/or protein-containing solutions. Alternatively, the low binding surfaces can be produced by applying the non-ionic surfactant to the mold surfaces which contact molten polymer and form the polymer into a desired shape, e.g., into a multi-well plate, a... |
| Simultaneous aspirator and dispenser for multiwell plates and similar devices | 20080115567 | 20080522 |
| A device for simultaneously dispensing a solution containing a sample to a test device and aspirating the sample from the device. In one embodiment the device is hand-held and includes a control mechanism, e.g., button for activating the dispensing and aspiration of the sample. The testing device may take the form of a multi-well plate with a plurality of wells arranged in rows and columns, with the bottom of the wells formed as a photonic crystal biosensor. The device can be configured with dispense and aspirate manifolds and associated dispense and aspirate ports which are positioned in all the wells along a row or column of wells so as to simultaneously aspirate and dispense solution to all the wells in a row or column of the... |
| Porous substrate plates and the use thereof | 20080119370 | 20080522 |
| A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.
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| Multi-channel design for a liquid-handling pipette | 20080101996 | 20080501 |
| A multi-channel liquid-handling pipette is provided having a top portion and a bottom portion. The top portion is disposed with a plurality of inlets and the bottom portion is disposed with a plurality of pipetting heads for mounting tips. Each of the inlets is connected to a syringe or an apparatus with the functions of both quantitatively aspirating and dispensing liquid and being in communication with the corresponding pipetting head. Each of the tips corresponds to a respective well of a multi-well plate. The multi-well plate is arranged as a 6×4, 12×8 or 24×16 array having a channel spacing of 18 mm, 9 mm and 4.5 mm between two adjacent wells, respectively, while the pipetting heads are arranged as a 6×4 array having an 18 mm... |
| Systems for screening pharmaceutical chemicals | 20080038812 | 20080214 |
| An apparatus for culturing a bio-artificial tissue including a multi-well plate and a scaffold is disclosed herein. Also disclosed is a tissue indentation system that includes a probe, an isometric force transducer, a computer and a computer-controlled motor. A tissue response system is provided and has a well including a scaffold with a bio-artificial tissue suspended on the scaffold and the tissue indentation system.
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| Device and method for high-throughput quantification of mrna from whole blood | 20080009009 | 20080110 |
| Disclosed are a method, device kit, and automated system for simple, reproducible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates.
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| Insert with concavity for organic culture and imaging | 20070292939 | 20071220 |
| A plastic culture insert with a concavity replaces glass coverslip for supporting organic culture. It can be freely placed into and removed from a multi-well plate. The concavity anchors a tissue via collagen polymerization. The concavity is then, after organic culture, sealed by a regular slide for data storage and microscopy imaging analysis.
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| Biochemical assay detection in a liquid receptacle using a fiber optic exciter | 20070263954 | 20071115 |
| Biochemical assays that are performed in cuvettes and in the wells of multi-well plates and that utilize excitation and light emission as labels for detection are enhanced by an illumination and detection system that supplies excitation light through an optical fiber that transmits excitation light from an excitation light source to the cuvette or well. Emission light produced by the excitation is then collected by a collimating lens and converted to a signal that is compiled by conventional software for analysis. The optical fiber and collimating lens can either be on the same side of the receptacle (generally the open side) or on opposite sides, i.e., one above and the other below. When the optical fiber and the collimating lens are both on the open side... |
| Multi-well plate | 20070264164 | 20071115 |
| A Multi-well plate for mixing a fluid, comprises a plurality of wells. Each of the wells has an opening arranged at a top side of the multi-well plate. At least one of the wells has a cross-section parallel to the top side having an elongated shape. The multi-well plate comprises 384 wells arranged in 16 rows and 24 columns or 96 wells arranged in 8 rows and 12 columns. By means of such a multi-well plate a faster and therefore more efficient mixing is possible using standard mixing devices such as shakers.
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| Assay apparatuses, methods and reagents | 20070231217 | 20071004 |
| We describe apparatuses, systems, method, reagents, and kits for conducting assays as well as process for their preparation. They are particularly well suited for conducting automated sampling, sample preparation, and analysis in a multi-well plate assay format. For example, they may be used for automated analysis of particulates in air and/or liquid samples derived therefrom in environmental monitoring.
... |
| Device and method for high-throughput quantification of mrna from whole blood | 20070224631 | 20070927 |
| Disclosed are a method, device kit, and automated system for simple, reproducible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates.
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| Device and method for high-throughput quantification of mrna from whole blood | 20070224632 | 20070927 |
| Disclosed are a method, device kit, and automated system for simple, reproducible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates.
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| Sample plate support of adjustable angular orientation | 20070202018 | 20070830 |
| A platform that supports a sample plate such as a microtiter plate, a multi-well plate of any size, or a glass slide with sample spots distributed over its surface, and presents the plate for assay detection by a movable scanning head that has a field depth on the millimeter scale is leveled or otherwise adjusted in a planar orientation by an apparatus that includes a rocker plate, position sensors, and motorized risers arranged on the apparatus to provide the rocker plate with tilting capability along either or both or two orthogonal axes.
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| Methods for producing low binding surfaces | 20070196910 | 20070823 |
| Hydrophobic polymer surfaces whose level of protein binding is less than about 50-80 ng/cm2 are achieved by: (1) applying a coating solution composed of a solvent and a non-ionic surfactant having a HLB number of less than 5 to the surface; and (2) drying the surface to remove the solvent and thereby bring the surfactant into direct contact with the hydrophobic polymer. The combination of a low HLB number and the drying step have been found to produce low binding surfaces which can withstand multiple washes with water and/or protein-containing solutions Alternatively, the low binding surfaces can be produced by applying the non-ionic surfactant to the mold surfaces which contact molten polymer and form the polymer into a desired shape, e.g., into a multi-well plate, a... |
| Novel cell-based assays for g-protein-coupled receptor-mediated activities | 20070178483 | 20070802 |
| Disclosed are compositions and methods for their use, such as in identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers.
... |
| Methods of screening agents for activity using teleosts | 20070143865 | 20070621 |
| The present invention provides methods of screening an agent for activity using teleosts. Methods of screening an agent for angiogenesis activity, toxic activity and an effect cell death activity in teleosts are provided. Methods of screening an agent for an activity in the brain or central nervous system in zebrafish are provided. The invention further provides high throughput methods of screening agents in multi-well plates.
... |
| Scaffold carrier cartridge | 20070082390 | 20070412 |
| A scaffold handling system comprising a multi-well carrier including an array of well units wherein, in each well unit, an independent scaffold or tissue slice may be held and a biological experiment may be performed. The carrier may include any number of well units. In operation, the scaffold handling system is configured and dimensioned to mate with a multi-well plate.
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| Multiwell plate with modified rib configuration | 20070077181 | 20070405 |
| A multiwell plate utilizing support structures outside a defined analytical zone is disclosed. Resultant plates have the modified structures located on an underside of the multi-well plate permissive to stacking of the plates and also preventing interference with analytical methods.
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| Methods and apparatus for identifying allosterically regulated ribozymes | 20070077571 | 20070405 |
| Compositions and methods are provided to use regulatable aptazymes for controlling gene expression and in assays to detect the presence of ligands or to detect activation by an effector of an aptazyme bound to a solid support such as a substrate or multi-well plate. Regulatable aptazymes are ribozymes that are allosterically regulated by an effector molecule. Also disclosed are compositions and methods for automating the selection procedures of the present invention.
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| Multi-well plate guide protector and method for multi-well dispensing | 20070009396 | 20070111 |
| The invention discloses a multi-well plate guide protector for single- and multi-well dispensing in a multi-well plate. The guide protector comprises a protecting cover having a height and dimension adapted for protecting the multi-well plate. The protecting cover has a dispensing window through the protecting cover adapted for allowing accurate and easy dispensing fluid in the well, row or column, of interest in the multi-well plate therethrough. The guide protector further comprises a position coupling coupled to the protecting cover. The position coupling is configured to move along on a position track on the multi-well plate for guiding the movement of the protecting cover so that the dispensing window can align with a targeted well in the multi-well plate.
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| Substrates for isolating, reacting and microscopically analyzing materials | 20060286550 | 20061221 |
| An immobilizing device for biological material comprises a rigid support (12) carrying a substrate layer (20, 20′) of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron, and micro-porous, ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably less than 3 micron, 2 or 1 micron are shown, which may be segmented by isolating moats M. The substrate layer is on a microscope slide (302), round disc (122), bio-cassette, at the bottom of a well of a multiwell plate, and as a coating inside a tube. Fluorescence or luminescence intensity and geometric calibration spots (420) are shown. Reading is... |
| Particulate separation filters and methods | 20060228265 | 20061012 |
| The present invention provides a novel fluid separator apparatus adapted for us with a multi-well plate. The separator is preferably adapted to separate fluid from particulate, amorphous or viscous material present in the fluid. The separator may be used in biological, immunological, histological biomarker and genomic detection assays and screening assays as well as for medical and chemical applications.
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| Methods, devices and kits for multiplex blotting of biological samples from multi-well plates | 20060211011 | 20060921 |
| The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.
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| Device and method for high-throughput quantification of mrna from whole blood | 20060199212 | 20060907 |
| Disclosed are a method, device kit, and automated system for simple, reproducible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates.
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| Apparatus used to prevent cross-contamination along a platform and methods of manufacturing the same | 20060171849 | 20060803 |
| Disclosed herein is an apparatus designed to prevent cross-contamination of a fluid along the surface of a platform like a laser desorption target or multi-well plate. In particular, the apparatus comprises a cover slip that is disposed in apposition to the platform. This cover slip has an upper surface and a lower surface, wherein the lower surface is in apposition to the platform. Optionally, the cover slip has one or more defined orifices that extend from the upper surface through to the lower surface such that fluid communication is maintained from the upper surface of the cover slip to the surface of the platform interfacing with the lower surface of the cover slip.
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| Multi-well plate with alignment grooves for encoded microparticles | 20060160208 | 20060720 |
| A method and apparatus are provided for aligning optical elements or microbeads, wherein each microbead has an elongated body with a code embedded therein along a longitudinal axis thereof to be read by a code reading device. The microbeads are aligned with a positioning device so the longitudinal axis of the microbeads is positioned in a fixed orientation relative to the code reading device. The microbeads are typically cylindrically shaped glass beads between 25 and 250 microns (μm) in diameter and between 100 and 500 μm long, and have a holographic code embedded in the central region of the bead, which is used to identify it from the rest of the beads in a batch of beads with many different chemical probes. A cross reference is... |
| Substrates for isolating reacting and microscopically analiyzing materials | 20060134606 | 20060622 |
| An immobilizing device for biological material comprises a rigid support (12) carrying a substrate layer (20, 20′) of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron, and micro-porous, ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably less than 3 micron, 2 or 1 micron are shown, which may be segmented by isolating moats M. The substrate layer is on a microscope slide (302), round disc (122), bio-cassette, at the bottom of a well of a multiwell plate, and as a coating inside a tube. Fluorescence or luminescence intensity and geometric calibration spots (420) are shown. Reading is... |
| Methods of screening agents for activity using teleosts | 20060123487 | 20060608 |
| The present invention provides methods of screening an agent for activity using teleosts. Methods of screening an agent for angiogenesis activity, toxic activity and an effect cell death activity in teleosts are provided. The invention further provides high throughput methods of screening agents in multi-well plates.
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| Apparatus for minimizing evaporation and/or condensation of samples occurring in tubes of multi-well plate mounted to pcr thermo cycler | 20060115891 | 20060601 |
| The present invention relates to an automated apparatus for effectively preventing evaporation of samples in reaction tubes during DNA amplification (i.e., Polymerase Chain Reaction) and DNA sequencing processes performed in a thermo cycler. The automated apparatus of the present invention comprises a plurality of plungers corresponding to the plurality of tubes, respectively, and a coupling member to which the plurality of plungers are simultaneously coupled and fixed. Each of the plurality of plungers includes a rod with a predetermined length, a head which is formed at an upper end of the rod to easily receive an actuating force needed for vertical movement of the plunger into the relevant tube, and an elastic member which is formed around a lower end of the rod to minimize a... |
| Perfusion bioreactors for culturing cells | 20060110822 | 20060525 |
| A bioreactor system comprising a multi-well platform including an array of bioreactor units. The bioreactor system comprises a perfusion unit and a fluid source unit fluidly interconnected by a pumping unit. The perfusion unit comprises a multi-well plate including a plurality of main chambers configured to house or contain a cell culture and in each bioreactor unit an independent cell study or experiment may be performed.
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| Method and apparatus for applying a pressure differential to a multi-well plate | 20060088448 | 20060427 |
| A method and apparatus for moving material in a multi-well plate includes applying positive and negative pressure to one or more wells in the plate, e.g., to enhance a flow rate of material from the well through a filter element. Wells requiring application of positive pressure may be identified, e.g., by image analysis performed by a machine vision system. Those wells determined to require enhanced throughput may have negative and positive pressure applied to the well, while other wells have only negative pressure applied.
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| Multi-well plates | 20060083664 | 20060420 |
| A test panel for use in preparing a coating array, includes a plate having a non-corrosive surface and a plurality of raised edges integrally formed as part of the non-corrosive surface. The raised edges define a plurality of wells. Also disclosed is a method of testing coatings placed on a panel.
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