|| List of recent Mass Spectrometry-related patents
| Method for identifying microorganisms via mass spectrometry and score normalization|
Where: m is the distance calculated for the reference microorganism; n(m. .
| Aluminum-polymer resin composite and method for producing the same|
Disclosed is an aluminum-polymer resin composite. The composite includes i) aluminum and ii) a polymer resin bonded to the aluminum after modification of the aluminum surface with at least one surface modifier selected from the group consisting of sulfur-containing diazole derivatives, sulfur-containing diamine derivatives, sulfur-containing thiol derivatives, sulfur-containing pyrimidine derivatives, and sulfur-containing silane coupling agents.
| Multinotch isolation for ms3 mass analysis|
A mass spectrometry method for analyzing isobarically-labeled analyte compounds comprising (a) ionizing compounds including the isobarically-labeled analyte compounds to generate a plurality of precursor ion species comprising different respective m/z ratios, (b) isolating a precursor ion species, (c) fragmenting the precursor ion species to generate a plurality of first-generation fragment ion species comprising different respective m/z ratios, and (d) selecting and co-isolating two or more of the first-generation product-ion species, the method characterized by: (e) fragmenting all of the selected and isolated first-generation product ion species so as to generate a plurality of second-generation fragment ion species including released label ions; (f) generating a mass spectrum of the second-generation fragment ion species; and (g) generating quantitative information relating to at least one analyte compound based on peaks of the mass spectrum attributable to the released label ions.. .
| Systems, devices, and methods for sample analysis using mass spectrometry|
A mass spectrometry system for screening a sample for one or more analytes includes a pre-mass spectrometry screening apparatus configured to pre-screen an ionized sample to generate output correlated to the composition of the sample, and a mass spectrometer. A sample gate is opened to allow flow of at least a portion of the ionized sample to the mass spectrometer and closed to prevent flow of the ionized sample to the mass spectrometer.
|Identification of related peptides for mass spectrometry processing|
A method of identifying a related peak set from ms1 spectra data is provided. An intensity peak is selected from ms1 spectra data generated for a sample by a tandem mass spectrometer.
|Srm/mrm assay for the ephrin typa-a receptor 2 protein|
Specific peptides, and derived ionization characteristics of the peptides, from the ephrin type-a receptor 2 (epha2) protein are provided that are particularly advantageous for quantifying the epha2 protein directly in biological samples that have been fixed in formalin by the method of selected reaction monitoring (srm) mass spectrometry, or what can also be termed as multiple reaction monitoring (mrm) mass spectrometry. Such biological samples are chemically preserved and fixed and are selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (ffpe) tissue/cells, ffpe tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded.
|Single crystal cvd synthetic diamond material|
A single crystal cvd synthetic diamond material comprising: a total as-grown nitrogen concentration equal to or greater than 5 ppm, and a uniform distribution of defects, wherein said uniform distribution of defects is defined by one or more of the following characteristics: (i) the total nitrogen concentration, when mapped by secondary ion mass spectrometry (sims) over an area equal to or greater than 50×50 μm using an analysis area of 10 μm or less, possesses a point-to-point variation of less than 30% of an average total nitrogen concentration value, or when mapped by sims over an area equal to or greater than 200×200 μm using an analysis area of 60 μm or less, possesses a point-to-point variation of less than 30% of an average total nitrogen concentration value; (ii) an as-grown nitrogen-vacancy defect (nv) concentration equal to or greater than 50 ppb as measured using 77k uv-visible absorption measurements, wherein the nitrogen-vacancy defects are uniformly distributed through the synthetic single crystal cvd diamond material such that, when excited using a 514 nm laser excitation source of spot size equal to or less than 10 μm at room temperature using a 50 mw 46 continuous wave laser, and mapped over an area equal to or greater than 50×50 μm with a data interval less than 10 μm there is a low point-to-point variation wherein the intensity area ratio of nitrogen vacancy photoluminescence peaks between regions of high photoluminescent intensity and regions of low photolominescent intensity is <2× for either the 575 nm photoluminescent peak (nv0) or the 637 nm photoluminescent peak (nv); (iii) a variation in raman intensity such that, when excited using a 514 nm laser excitation source (resulting in a raman peak at 552.4 nm) of spot size equal to or less than 10 μm at room temperature using a 50 mw continuous wave laser, and mapped over an area equal to or greater than 50×50 μm with a data interval less than 10 μm, there is a low point-to-point variation wherein the ratio of raman peak areas between regions of low raman intensity and high raman intensity is <1.25×; (iv) an as-grown nitrogen-vacancy defect (nv) concentration equal to or greater than 50 ppb as measured using 77k uv-visible absorption measurements, wherein, when excited using a 514 nm excitation source of spot size equal to or less than 10 μm at 77k using a 50 mw continuous wave laser, gives an intensity at 575 nm corresponding to nv0 greater than 120 times a raman intensity at 552.4 nm, and/or an intensity at 637 nm corresponding to nv− greater than 200 times the raman intensity at 552.4 nm; (v) a single substitutional nitrogen defect (ns) concentration equal to or greater than 5 ppm, wherein the single substitutional nitrogen defects are uniformly distributed through the synthetic single crystal cvd diamond material such that by using a 1344 cm−1 infrared absorption feature and sampling an area greater than an area of 0.5 mm2, the variation is lower than 80%, as deduced by dividing the standard deviation by the mean value; (vi) a variation in red luminescence intensity, as defined by a standard deviation divided by a mean value, is less than 15%; (vii) a mean standard deviation in neutral single substitutional nitrogen concentration of less than 80%; and (viii) a colour intensity as measured using a histogram from a microscopy image with a mean gray value of greater than 50, wherein the colour intensity is uniform through the single crystal cvd synthetic diamond material such that the variation in gray colour, as characterised by the gray value standard deviation divided by the gray value mean, is less than 40%.. .
|Methods of detecting reverse triiodothyronine by mass spectrometry|
Provided are methods for determining the amount of reverse t3 in a sample using mass spectrometry. The methods generally involve ionizing reverse t3 in a sample and detecting and quantifying the amount of the ion to determine the amount of reverse t3 in the sample..
|Use of windowed mass spectrometry data for retention time determination or confirmation|
A scan of a separating sample is received by a mass spectrometer at each interval of a plurality of intervals. The spectrometer performs at each interval one or more mass spectrometry scans.
|Techniques for quantification of samples|
Techniques are described for quantification of molecules in a sample. Mass spectrometry is performed to obtain ionization intensities for precursor and product ions originating from a particular molecule.
|Atmospheric pressure ion source for mass spectrometry|
A multiple function atmospheric pressure ion source interfaced to a mass spectrometer comprises multiple liquid inlet probes configured such that the sprays from two or more probes intersect in a mixing region gas phase sample ions or neutral species generated in the spray of one probe can react with reagent gas ions generated from one or more other probes by such ionization methods as electrospray, photoionization, corona discharge and glow discharge ionization. Reagent ions may be optimally selected to promote such processes as atmospheric pressure chemical ionization of neutral sample molecules, or charge reduction or electron transfer dissociation of multiply charged sample ions.
|Nanomanipulation coupled nanospray mass spectrometry (nms)|
A coupled nanomanipulation and nanospray mass spectrometry (nms) system for single cell, single organelle, and ultra-trace molecular analysis is disclosed herein. The system primarily comprises a bio-workstation coupled to a nms.
|Methods, apparatus, and system for mass spectrometry|
A miniature, low cost mass spectrometer capable of unit resolution over a mass range of 10 to 50 amu. The mass spectrometer incorporates several features that enhance the performance of the design over comparable instruments.
|Diathermy knife ionisation source|
A method of detecting one or more compounds, chemicals or contaminants in a substrate by mass spectrometry is disclosed. A non-living substrate is analysed by contacting the substrate with a diathermy knife.
|Determining the geographic origin of metals|
A method of determining the geographic origin of a metal can comprise measuring a first isotope and a second isotope of the metal by high-resolution mass spectrometry; calculating a ratio of the first isotope and the second isotope; comparing the ratio to native ratios of isotopes of the metal of native samples from a plurality of geographic locations using a database; and matching the ratio to a geographic location.. .
|Srm/mrm assay for the insulin receptor protein|
Specific peptides, and derived ionization characteristics of the peptides, from the insulin receptor protein (ir), and its isoforms ir-a and ir-b, that are particularly advantageous for quantifying the ir protein, ir-a isoform and/or ir-b isoform, directly in biological samples that have been fixed in formalin by the method of selected reaction monitoring (srm) mass spectrometry, or what can also be termed as multiple reaction monitoring (mrm) mass spectrometry. Such biological samples are chemically preserved and fixed and are selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (ffpe) tissue/cells, ffpe tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded.
There is provided an ion reflector for use with a mass spectrometer for directing a flow of ions between two distinct axes of travel. The reflector includes an electric field capable of causing a flow of ions focused through a first spatial region to be focused toward a second spatial region, whereby the first and second spatial regions are aligned with respective axes of travel..
|System and method for applying curtain gas flow in a mass spectrometer|
A system of mass spectrometry is disclosed having an ion source for generating ions at substantially atmospheric pressure. The system has a sampling member with an orifice disposed therein.
|Intelligent background data acquisition and subtraction|
A scan of a separating sample mixture is received from a mass spectrometer at each interval of a plurality of intervals. It is determined at a first interval that a received mass spectrometry scan at the first interval and one or more preceding received mass spectrometry scans include a varying ion signal that represents an ion of a known compound and has an intensity above a threshold level.
|New use for a compound as a matrix in the specific detection, identification and/or quantification of alkaloids by maldi-tof mass spectrometry|
There is provided (i) a method of analysing small molecules that may have a mass of <800 da, in particular alkaloids, said method being generally referred to as maldi-tof-ms (or maldi time-of-flight mass spectrometry), which is an acronym for a method of analysis by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Also provided is (ii) a molecule according to formula (i) and the use of the molecule as a matrix in the analysis method..
|Method for measuring acetic acid concentration in blood plasma|
The present invention relates to providing a simple and highly reproducible method for measuring the concentration of acetic acid in blood plasma by using a gas chromatography/mass spectrometry (gc/ms), and more specifically relates to a method for measuring the concentration of acetic acid in blood plasma by using a gas chromatography/mass spectrometry (gc/ms), which comprises extracting acetic acid in blood plasma with methyl-tert-butyl ether (mtbe).. .
|Capturing of cell fluid and analysis of its components under observation of cells and instruments for the cell fluid capturing and the analysis|
A method captures cellular components from a single cell and performs mass spectrometry on the components. The method includes inserting a nanospray ionization capillary tip into a specific region of the cell under observation with a microscope.
|Quantification of transthyretin and its isoforms|
The present invention relates to assays and methods for the detection of transthyretin and its isoforms. Specifically, the assays and methods of the present invention embrace liquid chromatography and mass spectrometry.
|Improvements in or relating to mass spectrometry|
There is provided an ion guide arrangement comprising a guide assembly comprising a plurality of elongate members arranged so as to be spaced about a common axis. The elongate members are capable of being in electrical association with one another so as to guide a stream of ions along an intended pathway substantially aligned with the axis.
|Simultaneous inorganic mass spectrometer and method of inorganic mass spectrometry|
An inorganic mass spectrometer capable of measuring a relevant and large or the full mass spectral range simultaneously may include a suitable ion source (e.g., an icp mass spectrometer with an icp ion source), an ion transfer region, ion optics to separate ions out of a plasma beam, a mattauch-herzog type mass spectrometer with a set of charged particle beam optics to condition the ion beam before an entrance slit, and a solid state multi-channel detector substantially separated from ground potential and separated from the potential of the magnet.. .
|Covalently functionalized nanodiamond-based maldi matrices and methods of use thereof|
The present disclosure relates to functionalized nanodiamonds comprising at least one maldi matrix covalently bonded to a nanodiamond and compositions comprising the same. The present disclosure also relates to methods of performing matrix-assisted laser desorption/ionization-mass spectrometry (maldi-ms), for example on small molecules, using matrices comprising at least one maldi matrix covalently bonded to a nanodiamond..
|Method and apparatus for leak testing containers|
Close containers which are filled with a consumer product are tested on leakiness by means of mass spectrometry (10) in that an impact (an(p)) by the consumer product (p) upon the surrounding atmosphere (a(p)) of the container to be leak tested is monitored by the mass spectrometry (10).. .
|Categorisation of biological deposits using matrix assisted laser desorption ionisation mass spectrometry|
A method of categorising a human according to pre-determined categories using matrix assisted laser desorption ionisation mass spectrometry (maldi-ms) is disclosed. A method is provided to discriminate humans based on gender, for example, by comparing maldi-ms sample spectral data in the m/z range 2,000 to 30,000 and comparing these sample spectral data with reference spectral data obtained from pre-categorised humans..
|Improved capillary electrophoresis-electrospray ionization-mass spectrometry system|
Aspects of the innovations presented herein relate to improved systems that in some embodiments perform capillary electrophoresis (ce) and ce in conjunction with electrospray ionization (esi) as an input to a mass spectrometry system (ms). Some embodiments use a high voltage isolated ce power supply that is configured to float on the high voltage output of an esi-ms power supply, with a protective resistance in the esi-ms path, as well as dc/dc converter isolation and communication system isolation for the isolated ce power supply.
|Chromatograph mass spectrometry data processing apparatus|
Even when only mass spectra wherein the reproducibility of peak intensities is low are obtained in a mass spectrometry apparatus using, for example, a maldi ion source, the correction of shifts in retention time using tics for a plurality of specimens is performed with good precision. For each mass spectrum, variable scaling is executed which combines such first scaling as to equalize the extent of variations in signal intensity values in one mass spectrum, among different mass spectra, and second scaling for performing weighting according to relative variations in signal intensity values for each mass spectrum (s3).
|Multiplexed detection with isotope-coded reporters|
Some aspects of this invention provide reagents and methods for the sensitive, quantitative and simultaneous detection of target analytes in complex biological samples by liquid chromatography tandem mass spectrometry (lc ms/ms). Some aspects of this invention provide affinity reagents encoded with mass reporters for the sensitive and quantitative translation of an analyte of interest into a mass tag.
|Apparatus for improved immunosuppressant drug monitoring|
A liquid chromatography/mass spectrometry system includes: a source of a first mobile phase solvent consisting essentially of water plus 10 mm ammonium formate plus 0.05% formic acid; a source of a second mobile phase solvent consisting essentially of methanol plus 10 mm ammonium formate plus 0.05% formic acid; a chromatography column comprising a length of 30 mm or less of a stationary phase comprising an 8-carbon alkyl chain material bonded to 2.6 μm diameter particles having solid silica cores surrounded by porous silica outer layers; an electrospray ion source of a mass spectrometer fluidically coupled to the chromatography column so as to generate ions therefrom; a mass analyzer of the mass spectrometer operable to quantitatively detect the ions; and a programmable processor electronically coupled to the mass analyzer and comprising instructions operable to determine, based on the ion detection, a concentration of everolimus, sirolimus, tacrolimus, or cyclosporin a.. .
|Apparatus for elemental analysis of particles by mass spectrometry|
A mass spectrometer has a particle introduction system and a vaporizer, atomizer, and ionizer configured to produce ions from elements associated with the particle. An ion mass-to-charge ratio analyzer is configured to separate ions according to their mass-to-charge ratio.
|Computer-assisted structure identification|
The invention relates to a method for analysing mass spectral data obtained from a sample in gc×gc (2-dimensional) mass spectrometry, comprising: (a) comparing mass spectral data of an analyte with mass spectral data of candidate compounds of known structure in a data library; (b) identifying a plurality of candidate compounds from the library based on similarities of mass spectral data; (c) predicting, for each candidate compound, a value of at least one analytical property using a quantitative model based on a plurality of molecular descriptors; and (d) calculating a match score for each candidate compound based on the value predicted in step (c) and a measured value of the analytical property for the analyte.. .
|Nonaqueous electrolyte air battery|
A nonaqueous electrolyte air battery has a positive electrode comprises at least a catalyst which activates oxygen, a conductive material and a binder, when a thermal decomposition starting temperature of the binder is t1° c. And a thermal decomposition ending temperature of the binder is t2° c.
|Nonaqueous electrolyte air battery|
A nonaqueous electrolyte air battery has s positive electrode comprises at least a catalyst which activates oxygen, a conductive material and a binder, when a thermal decomposition starting temperature of the binder is t1° c. And a thermal decomposition ending temperature of the binder is t2° c.
|Adaptive and targeted control of ion populations to improve the effective dynamic range of mass analyser|
A method of mass spectrometry is disclosed wherein one or more relatively abundant or intense species of ions in a first population of ions are selectively attenuated so as to form a second population of ions. The total ion current of the second population of ions is then adjusted so that the ion current corresponding to ions which are onwardly transmitted to a mass analyser comprising an ion detector is within the dynamic range of the ion detector..
|Methods for detecting vitamin c by mass spectrometry|
Provided are methods for determining the amount of vitamin c in a sample using mass spectrometry. The methods generally involve ionizing vitamin c in a sample and detecting and quantifying the amount of the ion to determine the amount of vitamin c in the sample..
|Photo-dissociation of proteins and peptides in a mass spectrometer|
A method of mass spectrometry is disclosed comprising directing first photons from a laser onto ions located within a 2d or linear ion guide or ion trap. The frequency of the first photons is scanned and first photons and/or second photons emitted by the ions are detected.
|Cell identification device and program|
An apparatus that identifies the type of a test cell based on a result obtained by performing mass spectrometry on the test cell includes a higher-level database, which contains mass lists that each list ion mass values of constituent components of a known cell, and a lower-level database, which contains partial mass lists that each list only strain-specific ion mass values out of the ion mass values. The higher-level database is first searched for a test mass list which is created from the result of the mass spectrometry performed on the test cell, and based on a result of the search, an organism species to be searched in the following search operation is determined.
|Isotopic labeling for the measurement of global protein levels and turnover in vivo|
An entire complement or plurality of isotopically labeled amino acids are introduced into the diet of a test subject. Sufficient amounts of the isotopically labeled amino acids are provided to the subject in order to ensure that the subject incorporates a large percentage of isotopically labeled amino acids into newly synthesized proteins.
|Laser ablation cell|
A laser ablation cell (1) comprises a flow channel (11) having an essentially constant cross-sectional area so as to ensure a strictly laminar flow in the flow channel. A sample chamber (21) is provided adjacent to a lateral opening (14) of the flow channel.
|Mass spectrometers comprising accelerator devices|
A method of mass spectrometry is disclosed comprising providing a flight region for ions to travel through and a detector or fragmentation device. A potential profile is maintained along the flight region such that ions travel towards the detector or fragmentation device.
|Biomarker for ovarian cancer ctap3-related proteins|
The present invention provides a protein-based biomarker that is useful in qualifying ovarian cancer status in a patient. In particular, the biomarker of this invention is useful to classify a subject sample as ovarian cancer or non-ovarian cancer.
|Methods for making microarrays and their uses|
The present invention provides microarrays that can be analysed by more than one technique using a non-covalent ligand attachment strategy to solid supports such as indium tin oxide (ito) covered transparent glass slides. This provides, inter alia, glycan arrays on a micrometer scale which allow multimodal readout by maldi-tof-ms, fluorescence and optical microscopy.
|Chemical identification using a chromatography retention index|
Provided herein is technology relating to identifying unknown compounds and particularly, but not exclusively, to methods and systems for identifying unknown compounds by gas chromatography-mass spectrometry by use of retention index as a second dimension for identification.. .
|Method for determining derivatized analytes in a separated biological fluid|
The present invention comprises methods for determining the presence, amount, or concentration of analytes of interest including vitamin d and other secosteroids from biological samples, through derivatization, with improved speed and ease of analysis and improved sensitivity to mass spectrometry. In a preferred embodiment, the present invention comprises methods for determining the presence, amount, or concentration of analytes of interest, including vitamin d and other secosteroids from whole human blood, through derivatization, and using a plasma collection device to facilitate collection, separation, and preparation of the sample for derivatization and analysis..
|Ion mobility mass spectrometry tags for quantitative applications and methods thereof|
Compound tags and shifting agents are provided that find use in ion mobility spectrometry (ims), mass spectrometry (ms), or a combination of ims and ms, and which can substantially increase separation of multiple components in complex samples and facilitate quantitative and multiplexed analyses. In some cases, the compounds include a linker and a normalizing group, each including a structural unit and separated by a cleaveable group, and a crown ether.
|Method of detecting mycobacterium tuberculosis complex by cell filtrate protein 10-loaded detonation nanodiamond|
A method of detecting mycobacterium tuberculosis complex (mtbc) in a culture media is provided, in which, the culture media containing the mtbc and a biomarker, such as cfp-10, secreted from the mtbc is provided, the culture media is filtered to obtain a filtrate, detonation nanodiamond particles are mixed with the filtrate to form biomarker-loaded detonation nanodiamond particles, and a mass spectrometry analysis process is performed on the biomarker-loaded detonation nanodiamond particles for detecting the biomarker.. .
|Portable field 3he/4he stable isotope detector for use in survey work and autonomous monitoring|
An instrument is described for measurements of the isotopic abundance of 3he and 4he stable isotopes remotely and in near real time. It is designed to work autonomously in the field in harsh environments, and is composed of modestly priced materials, vacuum and electronic subsystems for economical use as a stand-alone instrument.
|Orthogonal acceleration tof with ion guide mode|
Mass spectrometry systems include an electronic controller and a time-of-flight mass analyzer in communication with the electronic controller. The time-of-flight mass analyzer includes a pulsing region defining a channel that extends along an axis.
|Systems and methods for analyzing a sample using a mass spectrometry probe configured to contact the sample|
The invention generally relates to systems and methods for analyzing a sample using a mass spectrometry probe having a tip that is configured to contact a sample and retain a portion of the sample once the probe has been removed from the sample.. .
|Method and apparatus for control of a plasma for spectrometry|
A method of and apparatus for controlling the temperature of an inductively coupled or microwave induced plasma for optical emission spectrometry or mass spectrometry in which the intensities of two spectral lines of radiation emitted by the plasma are measured, and the power provided to sustain the plasma is adjusted so that the ratio of the intensities remains substantially constant.. .
|Microscale mass spectrometry systems, devices and related methods|
Mass spectrometry systems or assemblies therefore include an ionizer that includes at least one planar conductor, a mass analyzer with a planar electrode assembly, and a detector comprising at least one planar conductor. The ionizer, the mass analyzer and the detector are attached together in a compact stack assembly.
|Method of processing image charge/current signals|
A method of processing an image charge/current signal representative of trapped ions undergoing oscillatory motion. The method includes applying a validity test to each of a plurality of peaks in the image charge/current signal in the frequency domain, wherein applying the validity test to a peak in the image charge/current signal in the frequency domain includes determining whether a phase angle associated with the peak meets a predetermined condition.
|Integrated magnetron plasma torch, and related methods|
A plasma source for generating microwave-induced plasma includes a plasma torch integrated with a microwave energy source. The torch establishes a gas flow path from one side of the plasma source to the other side.
|Use of detection techniques for contaminant and corrosion control in industrial processes|
Industrial fluids may be monitored at the site of each industrial fluid by introducing a sample of the industrial fluid into a device employing a detection technique for detecting at least one composition within the sample. The detection technique may be or include surface enhanced raman scattering (sers), mass spectrometry (ms), nuclear magnetic resonance (nmr), ultraviolet light (uv) spectroscopy, uv spectrophotometry, indirect uv spectroscopy, contactless conductivity, laser induced fluorescence, and combinations thereof.
|Preparation enhancements and methods of use for maldi mass spectrometry|
Provided herein are compositions and methods useful for preparing and analyzing a sample on a substrate by matrix assisted laser desorption ionization (maldi) mass spectrometry (ms). In some embodiments, compositions provided herein comprise a substrate, matrix and nanoparticles, and sometimes comprise one or more additives and sometimes an analyte.
|Mass spectrometry (ms) identification algorithm|
A system includes a gas chromatograph configured to determine experimental chromatographic data including retention times associated with samples. The system also includes a mass spectrometer configured to determine experimental mass spectral data associated with samples.
|Method for fabricating stable-isotope-labeled target peptide fragment in mass spectrometry|
An object of the present invention is to provide a method for producing a stable isotope-labeled target peptide fragment in mass spectrometry, which achieves inexpensive and convenient production. As a solution to attain the object, the stable isotope-labeled target peptide fragment in mass spectrometry is produced using a method comprising the steps of: expressing a dna conjugate in a system having a stable isotope-labeled amino acid to thereby prepare a stable isotope-labeled protein, wherein the dna conjugate comprises: a tandemly linked dna in which two or more dnas encoding one or more types of target peptide fragments are linked in tandem; and a dna encoding a peptide fragment for concentration measurement; subjecting the stable isotope-labeled protein to fragmentation treatment with trypsin to prepare a stable isotope-labeled peptide fragment for concentration measurement and stable isotope-labeled target peptide fragments; quantifying the stable isotope-labeled peptide fragment for concentration measurement using a liquid chromatograph-tandem mass spectrometer (lc/ms/ms); and calculating the concentration of the stable isotope-labeled target peptide fragment of each type from the quantification value of the stable isotope-labeled peptide fragment for concentration measurement..
|Analytical method of post-translational modifications in hemoglobin|
An analytical method of post-translational modifications in hemoglobin is disclosed. The analytical method comprises the steps of providing a blood comprising the hemoglobin with post-translational modification of nitration, nitrosylation, or oxidation; performing an extraction process to the blood by an organic solvent; quantifying the hemoglobin by a fluorescent spectrometry; hydrolyzing the hemoglobin into a plurality of peptides by an enzyme; and using a nanoflow liquid chromatography-nano spray ionization tandem mass spectrometry to characterize and quantify the post-translational modifications of the hemoglobin..
Methods and materials relate to degradable detergents. The degradable detergents have degradable linkages that are cleaved when subjected to elevated temperature and/or reduced pressure.
|Automatic gain control with defocusing lens|
A method and apparatus for performing mass spectrometry using an electron source, an ion trap, and a voltage-controlled lens located between the electron source and the ion trap. A controller applies a voltage to the lens.
|General mass spectrometry assay using continuously eluting co-fractionating reporters of mass spectrometry detection efficiency|
The invention provides general methods for quantifying any conceivable compound including small organic molecules and biological molecules in mass spectrometric measurements. The methods include the use of chemical or biological reporters such as artificial polypeptides containing proteolytic cleavage sites, which provide proteolytic reporter peptides for standardization of mass spectrometric detection efficiency.
|Methods and apparatus for decomposing tandem mass spectra generated by all-ions fragmentation|
A method for tandem mass spectrometry of a plurality of eluting compounds comprises: (a) performing, during a time period, the steps of: ionizing the plurality of eluting compounds to generate a plurality of precursor ion species; introducing the plurality of precursor ions into a fragmentation cell operated at constant fragmentation energy so as to generate a plurality of product-ion species from at least a portion of the precursor ion species; and generating a mass spectrum of the plurality of product-ion species; and (b) recognizing matches between certain of the product ion species generated during the time period based on correlations between elution profiles of the product ion species.. .