|| List of recent Mass Spectrometer-related patents
|Method for fabricating stable-isotope-labeled target peptide fragment in mass spectrometry|
An object of the present invention is to provide a method for producing a stable isotope-labeled target peptide fragment in mass spectrometry, which achieves inexpensive and convenient production. As a solution to attain the object, the stable isotope-labeled target peptide fragment in mass spectrometry is produced using a method comprising the steps of: expressing a dna conjugate in a system having a stable isotope-labeled amino acid to thereby prepare a stable isotope-labeled protein, wherein the dna conjugate comprises: a tandemly linked dna in which two or more dnas encoding one or more types of target peptide fragments are linked in tandem; and a dna encoding a peptide fragment for concentration measurement; subjecting the stable isotope-labeled protein to fragmentation treatment with trypsin to prepare a stable isotope-labeled peptide fragment for concentration measurement and stable isotope-labeled target peptide fragments; quantifying the stable isotope-labeled peptide fragment for concentration measurement using a liquid chromatograph-tandem mass spectrometer (lc/ms/ms); and calculating the concentration of the stable isotope-labeled target peptide fragment of each type from the quantification value of the stable isotope-labeled peptide fragment for concentration measurement..
Methods and materials relate to degradable detergents. The degradable detergents have degradable linkages that are cleaved when subjected to elevated temperature and/or reduced pressure.
|Mass spectrometer ion trap having asymmetric end cap apertures|
An ion trap for a mass spectrometer is disclosed. The ion trap includes a ring electrode and first and second electrodes which are arranged on opposite sides of the ring electrode.
|Tandem ion trapping arrangement|
A mass spectrometer is disclosed comprising a first storage ion trap arranged upstream of a high performance analytical ion trap. According to an embodiment, ions are simultaneously scanned from both the first and second ion trap.
|Mass spectrum noise cancellation by alternating inverted synchronous rf|
A mass spectrometer comprising a controller configured to generate an rf signal to be applied to an electrode during the mass scan, wherein the electrode generates, based on the rf signal, an electric field to be applied to sample ions during a mass scan; an ion detector configured to detect sample ions passing through the electric field and generate a corresponding ion detection signal; and a sampling circuit configured to sample the ion detection signal; wherein the controller is configured to adjust a phase of the at least one rf signal relative to a sample timing of the sampling circuit and average successive mass scans to cancel a portion of the rf signal present in the ion detection signal.. .
|Methods and systems for applying end cap dc bias in ion traps|
A mass spectrometer for analyzing a sample utilizing an ion trap comprises an entrance end cap defining an entrance aperture configured to receive the sample entering the ion trap; a ring electrode defining a ring cavity configured to generate, based on a radio frequency (rf) voltage applied to the ring electrode, an electric field configured to trap the sample received through the entrance aperture; an exit end cap defining an exit aperture configured to receive sample ions exiting the ion trap; and an end cap controller configured to generate a bias control voltage for applying a dc bias potential to at least one of the entrance end or the exit end cap, wherein a value of the bias control voltage is based on an operational parameter of the mass spectrometer.. .
A mass spectrometer having a multi-stage differential pumping system with an ion lens provided in a partition wall separating a second intermediate vacuum chamber and a third intermediate vacuum chamber, the incircle radii of ion guides and the size of the opening of the ion lens are determined so that the circumferential edge of the opening is located outside the circumferential surface of a virtual tubular body straightly connecting the incircle at the rear edge of the second ion guide in the front stage and the incircle at the front edge of the third ion guide in the rear stage. Although the ion lens is located in between, the radio-frequency electric field created by the second ion guide can be effectively connected to the radio-frequency electric field created by the third ion guide through the opening of the ion lens..
|Systems and methods for calibrating mass spectrometers|
Systems and methods are disclosed for calibrating mass spectrometers. In accordance with one implementation, a system comprises a calibrant chamber within a housing of a mass spectrometer.
|Chemical analysis instrument with multi-purpose pump|
A mass spectrometer for analyzing a sample may include an analysis chamber for analyzing the sample and a first vacuum pump operably connected to the analysis chamber, wherein the first vacuum pump operates to create a first vacuum state. The mass spectrometer may also include a sample-preparation chamber operably connected to the analysis chamber and a second vacuum pump that operates to create a second vacuum state, wherein the first vacuum state is a lower pressure than the second vacuum state.
|Looped ionization source|
Looped ionization sources for ion mobility spectrometers are described. The ionization sources can be used to ionize molecules from a sample of interest in order to identify the molecules based on the ions.
|Multiple channel detection for time of flight mass spectrometer|
An ion detector for a time of flight mass spectrometer is disclosed comprising a single microchannel plate which is arranged to receive ions and output electrons. The electrons are directed onto an array of photodiodes which directly detects the electrons.
|Method to perform beam-type collision-activated dissociation in the pre-existing ion injection pathway of a mass spectrometer|
Described herein are methods and systems related to the use of the pre-existing ion injection pathway of a mass spectrometer to perform beam-type collision-activated dissociation, as well as other dissociation methods. The methods can be practiced using a wide range of mass spectrometer configurations and allows msn experiments to be performed on very basic mass spectrometers, even those without secondary mass analyzers and/or collision cells.
|Segmented planar calibration for correction of errors in time of flight mass spectrometers|
An ion detector system for a mass spectrometer is disclosed comprising an ion detector comprising an array of detector elements. The ion detector system is arranged to correct for tilt and non-linear aberrations in an isochronous plane of ions.
|Chromatograph mass spectrometer|
Sequential identification numbers are automatically provided and undisplayed, constant, unique numbers are assigned to each event registered in an analysis condition setting table 100. Since correspondence information between the identification numbers and the unique numbers changes due to the reassignment of identification numbers when an event is deleted from the table, event identification numbers for each compound are changed by referring to the correspondence information on a compound information table.
In order to solve a problem in a mass spectrometry that a distribution of an emitted ion and a substance distribution on the measurement object surface are different from each other, which is due to a shaded portion of a irregular surface which falls under a shadow of primary beam, a primary ion optical system of the present apparatus includes a deflection unit configured to deflect the primary ion in such a manner that the primary ion intersects a flight space of the secondary ion in the course of flight.. .
|Method and apparatus for mass spectrometry|
A method for analysing ions according to their mass-to-charge ratio and mass spectrometer for performing the method, comprising directing a collimated ion beam along an ion path from an ion source to an ion detector, causing a portion of the ion beam to contact one or more surfaces prior to reaching the ion detector, wherein the method comprises providing a coating on and/or heating the one or more surfaces to reduce variation in their surface patch potentials. The method is applicable to multi-reflection time-of-flight (mr tof) mass spectrometry..
|Techniques for automated performance maintenance testing and reporting for analytical instruments|
Techniques are described for performing performance maintenance on a mass spectrometer. Pre-maintenance testing is performed that automating execution of a test sequence in response to a first user interface selection.
|Tandem mass spectrometer and mass spectrometric method|
An ion trap is provided between a collision cell and a time-of-flight mass separator. During a time period in which precursor ions derived from the same compound are selected with a quadrupole mass filter, a collision energy is changed from one to another.
|Detection of membrane protein-therapeutic agent complexes by mass spectrometry|
According to the present invention, there is provided a method of detecting a complex comprising a membrane protein bound to a therapeutic agent by mass spectrometry. The method comprises: (a) providing a solution comprising a detergent micelle in which said complex is contained; (b) providing a mass spectrometer comprising a nanoelectrospray ionisation source, a mass analyser and a detector; (c) vaporising the solution using the nanoelectrospray ionisation source under conditions such that the complex is released from the micelle; (d) ionising the complex; (e) resolving the ionised complex using the mass analyser; and (f) detecting the resolved complex using the detector.
|Discontinuous atmospheric pressure interface|
A method of interfacing atmospheric pressure ion sources, including electrospray and desorption electrospray ionization sources, to mass spectrometers, for example miniature mass spectrometers, in which the ionized sample is discontinuously introduced into the mass spectrometer. Discontinuous introduction improves the match between the pumping capacity of the instrument and the volume of atmospheric pressure gas that contains the ionized sample.
|Mass spectrometer with bypass of a fragmentation device|
A method for analyzing a mixture of components includes forming precursor ions from the components, alternately causing the precursor ions to pass to and to by-pass a fragmentation device, to form product ions from the precursor ions that pass to the device and to form substantially fewer product ions from precursor ions that by-pass the device, and obtaining mass spectra from product ions received from the device and from precursor ions that by-passed the device. An apparatus for analyzing a sample includes an ion source for forming precursor ions from the components of the sample, a fragmentation device for forming product ions from the precursor ions, a by-pass device disposed upstream of the fragmentation device for switchable by-pass of the fragmentation device, and a mass analyzer..
A collision cell for a mass spectrometer arranged to receive ions for fragmentation in a chamber and comprising an activation ion generator configured to irradiate the received ions with activation ions of the same polarity as the received ions. The activation ion generator is preferably a plasma generator, configured to generate a plasma comprising the activation ions..
|Time-of-flight mass spectrometer|
A thin metal plate and two prismatic-bar-shaped metal members that are parallel to each other are alternately and repeatedly stacked, and the stack is sandwiched between two thick metal plates. Each contact surface is bonded to the counterpart surface by diffusion bonding to form an integrated multilayer body.
|Differentially pumped dual linear quadrupole ion trap mass spectrometer|
The present disclosure provides a new tandem mass spectrometer and methods of using the same for analyzing charged particles. The differentially pumped dual linear quadrupole ion trap mass spectrometer of the present disclose includes a combination of two linear quadrupole (lqit) mass spectrometers with differentially pumped vacuum chambers..
|Quadrupole mass spectrometer with enhanced sensitivity and mass resolving power|
A novel method and mass spectrometer apparatus is introduced to spatially and temporally resolve images of one or more ion exit patterns of a multipole instrument. In particular, the methods and structures of the present invention measures the ion current as a function of time and spatial displacement in the beam cross-section of a quadrupole mass filter via an arrayed detector.
A mass spectrometer is disclosed comprising an ion mobility spectrometer or separator and an ion guide arranged downstream of the ion mobility spectrometer or separator. A plurality of axial potential wells are created in the ion guide so that ions received from the ion mobility spectrometer or separator become confined in separate axial potential wells.
|Photo-dissociation of proteins and peptides in a mass spectrometer|
A method of mass spectrometry is disclosed comprising automatically and repeatedly performing multiple cycles of operation, wherein a cycle of operation comprises the steps of: (i) mass analysing first ions; (ii) exposing the first ions to a first photo-dissociation device to form a plurality of second ions and mass analysing the second ions; and (iii) exposing the first ions to a first photo-dissociation device to form a plurality of second ions, fragmenting the second ions to form a plurality of third ions and mass analysing the third ions.. .
|Mass spectrometer and mass spectrometric method|
A mass spectrometry using helium as cooling gas is performed to obtain a first mass spectrum (s1), and another mass spectrometry using argon, which is heavier than helium, as cooling gas is performed to obtain a second mass spectrum for the same sample (s2). Due to the difference between the two gases in terms of the effect of promoting dissociation of modifications, an ion peak originating from a target compound from which all the modifications have been dissociated will appear with a higher intensity on the second mass spectrum.
|Correction of asymmetric electric fields in ion cyclotron resonance cells|
The invention relates to a method and a device for optimization of electric fields in measurement cells of fourier transform ion cyclotron resonance mass spectrometers. The invention is based on the rationale that asymmetric electric fields with uniformly or non-uniformly perturbed field axes can appear in ion cyclotron resonance cells and therefore the axis of the magnetron orbit can become radially displaced.
|Radio-frequency-free hybrid electrostatic/magnetostatic cell for transporting, trapping, and dissociating ions in mass spectrometers|
Mass spectrometry cells include one or more interleaved magnetostatic and electrostatic lenses. In some examples, the electrostatic lenses are based on electrical potentials applied to magnetostatic lens pole pieces.
|Method and apparatus for improving ion transmission into a mass spectrometer|
An ion transfer device for transferring ions emerging from an electrospray ion source at atmosphere to a vacuum chamber includes an inner surface in the shape of a diverging conical duct. The ion transfer device has an entrance aperture for positioning proximate the exit port of the electrospray ion source emitter, the entrance aperture receiving the electrosprayed ions from the exit port of the electrospray ion source emitter at atmosphere, the diverging conical duct being an electrode toward which the ions migrate and having an exit aperture with an inner diameter larger than an inner diameter of its entrance aperture, the exit aperture enclosed in the vacuum chamber, the diverging conical duct transporting the ions from atmosphere to vacuum.
|Aperture gas flow restriction|
A mass spectrometer is disclosed comprising two vacuum chambers maintained at different pressures. The two vacuum chambers are interconnected by a differential pumping aperture.
|Ion guide with orthogonal sampling|
A mass spectrometer is disclosed comprising a rf ion guide wherein in a mode of operation a continuous, quasi-continuous or pulsed beam of ions is orthogonally sampled from the ion guide and wherein the continuous, quasi-continuous or pulsed beam of ions is not axially trapped or otherwise axially confined within the rf ion guide. The ion guide is maintained, in use, at a pressure selected from the group consisting of: (i) 0.0001-0.001 mbar; (ii) 0.001-0.01 mbar; (iii) 0.01-0.1 mbar; (iv) 0.1-1 mbar; (v) 1-10 mbar; (vi) 10-100 mbar; and (vii) >100 mbar..
|Fragmentation methods for mass spectrometry|
Apparatus and methods are provided that enable the interaction of low-energy electrons and positrons with sample ions to facilitate electron capture dissociation (ecd) and positron capture dissociation (pcd), respectively, within multipole ion guide structures. It has recently been discovered that fragmentation of protonated ions of many biomolecules via ecd often proceeds along fragmentation pathways not accessed by other dissociation methods, leading to molecular structure information not otherwise easily obtainable.
|Method for predicting whether a cancer patient will not benefit from platinum-based chemotherapy agents|
A testing method for identification whether a cancer patient is a member of a group or class of cancer patients that are not likely to benefit from administration of a platinum-based chemotherapy agent, e.g., cisplatin, carboplatin or analogs thereof, either alone or in combination with other non-platinum chemotherapy agents, e.g., gemcitabine and paclitaxel. This identification can be made in advance of treatment.
|Function switching with fast asynchronous acquisition|
A method of analysing a sample is disclosed comprising transmitting a first population of ions through a mass spectrometer and switching a state or mode of the mass spectrometer to produce a second population of ions. A sequential stream of mass spectra is acquired asynchronously with respect to switching the state or mode of the mass spectrometer.
|Arrangement for a removable ion-optical assembly in a mass spectrometer|
Presented is a mass spectrometer comprising an ion path along which ions are transported between different sections of the mass spectrometer, and further comprising an arrangement with a receptacle being located along the ion path in the mass spectrometer and a complementary mount for carrying a removable ion-optical assembly, such as a carrier of electrodes for a maldi ion source, wherein the mount can be removed from and reinserted into the receptacle in a plane approximately perpendicular to an ion path axis.. .
|Timing device and method|
The present invention provides a timing device, especially a timing device for use in mass spectrometers, for example tof mass spectrometers, for processing trigger signal data containing a trigger signal indicating the occurrence of a trigger event, the timing device having: a trigger signal deserialiser configured to receive trigger signal data containing a trigger signal indicating the occurrence of a trigger event as serial data and to output the trigger signal data as parallel data, and wherein suitably the timing device has a processing means configured to process trigger signal data outputted by the trigger signal deserialiser as parallel data.. .
|Methods for the detection of biologically relevant molecules and their interaction characteristics|
Methods for the detection of biologically relevant molecules that comprise concentrating such molecules into microscopic holes in a sheet of chemically inert material, restricting the openings, and measuring the electric current through the holes or the fluorescence near the hole openings. The electric current or fluorescence will change as the molecules diffuse out of the holes, providing a measure of the diffusion rate and thereby detecting the presence and characteristics of the molecules.
|High pressure mass spectrometry systems and methods|
Mass spectrometers and methods for measuring information about samples using mass spectrometry are disclosed.. .
|Compact mass spectrometer|
Mass spectrometers and methods for measuring information about samples using mass spectrometry are disclosed.. .
|Method for analyzing halogen oxoacids|
To quantitatively analyze halogen oxoacids such as bromic acid and perchloric acid, an hplc/ms in which a mass spectrometer is connected to the outlet of a column of a high performance liquid chromatograph (hplc) is used, and by using a reverse-phase column having an ion exchange function as the column, as well as a mixed liquid of an ammonium formate buffer solution and acetonitrile as the mobile phase, gradient analysis in which the concentration of ammonium formate in ammonium formate/acetonitrile is increased is performed. Thereby, a common hplc/ms apparatus configuration using no suppressor makes it possible to appropriately separate various halogen oxoacids and other components contained in a sample and to detect them at high sensitivity..
|Tandem mass spectrometer and tandem mass spectrometry method|
The invention relates to a tandem mass spectrometer comprising an ionisation source (1) that can produce ions; a mass analyser comprising an ion trap (2) arranged in such a way as to receive ions from the ion source and detection means that can detect ions leaving the ion trap according to the mass m to load z (m/z) ratio thereof; ion activation means for activating ions that can fragment at least some of the ions trapped in the ion trap; and coupling means arranged between the ion trap and said ion activation means. According to the invention, the ion activation means consist of a glow discharge lamp (4) that can generate a light beam oriented towards the ion trap (2), said light beam being electromagnetic radiation in the vacuum ultraviolet wavelength range with photon energies of between 8 ev and 41 ev in such a way as to fragment at least some of the ions trapped in the ion trap (2)..
|Method for the detection of incorrect deposition on a maldi sample support|
The invention relates to a method for the detection of incorrect deposition on a maldi sample support with several separate sample sites, where after the preparation on the sample support, an area located between two sample sites is sampled with a desorption laser, and a signal of an ion detector in a mass spectrometer is acquired in temporal relation to the sampling. The signal is examined for the presence of a signal which indicates incorrect deposition.
|Systems and methods for high throughput solvent assisted ionization inlet for mass spectrometry|
A multiplex system and method for achieving high throughput analysis of samples using solvent assisted ionization inlet includes an ionizing system with a heated inlet channel and a pressure differential across the inlet channel, pipet tips serially aligned with the inlet to a mass spectrometer, and a system of mapping data generated by mass spectrometry.. .
|Imaging mass spectrometer and method of controlling same|
An imaging mass spectrometer capable of reducing the dependence of the resolution of a projection image on mass is offered. Also, a method of controlling this spectrometer is offered.
|Compositions, methods, and kits for quantifying target analytes in a sample|
A method of quantifying a target analyte by mass spectrometry includes obtaining a mass spectrometer signal comprising a first calibrator signal, comprising a second calibrator signal, and potentially comprising a target analyte signal from a single sample comprising a first known quantity of a first calibrator, comprising a second known quantity of a second calibrator, and potentially comprising a target analyte. The first known quantity and the second known quantity are different, and wherein the first calibrator, the second calibrator, and the target analyte are each distinguishable in the single sample by mass spectrometry.
|Ion trap quadrupole mass filter|
An ion trap mass spectrometer is provided, including: an electron emitter; an ion trap storing ions generated by ionization resulting from an impact with electrons emitted from the electron emitter; a secondary ion filter for blocking out secondary ions generated due to ions selectively released by the ion trap; and a detector detecting ions selectively released from the ion trap, wherein the electron emitter, the ion trap, the secondary ion filter, and the ion detector are arranged on the same axis, so that a pure mass spectrum can be measured by excluding the secondary ions which are causes of background noise signals in the procedure of detection of the ions by the ion trap mass spectrometer.. .
|Method and system of identifying a sample by analysing a mass spectrum by the use of a bayesian inference technique|
A method and system for the identification and/or characterisation of properties of a sample using mass spectrometry. The method involves producing a measured data set from a sample using a mass spectrometer, deconvoluting the measured data set by bayesian inference to produce a family of plausible deconvoluted data sets, inferring an underlying deconvoluted data set from the family of plausible deconvoluted data sets and using the underlying deconvoluted data set to identify and/or characterise the sample..
A mass spectrometer is disclosed comprising a time of flight mass analyser. The time of flight mass analyser comprises an ion guide comprising a plurality of electrodes which are interconnected by a series of resistors forming a potential divider.
|Detection and quantification of polypeptides using mass spectrometry|
The invention relates to the detection and quantification of polypeptides using mass spectrometry. Specifically, the invention provides a method for testing whether a target polypeptide is present in a sample of a set of polypeptides, a method for deriving a value for distinguishing polypeptides of a set of polypeptides from each other, a database containing values for distinguishing each polypeptide of a set of polypeptides from each other, and an apparatus for configuring a mass scan of a mass spectrometer to test whether a target polypeptide of a set of polypeptides is present in a sample of the set..
|System for quantitative chemical analysis of samples, in particular in the medical field, with calibration of the instrumental response, and the corresponding method|
Analysis system for the quantitative chemical analysis of samples includes a detection equipment to detect the quantity of target analytes in the samples to be analyzed, which includes a chromatography system, an ion source and a mass spectrometer; a data processing system to process quantitative data of the target analytes in the samples analyzed, as detected by the detection equipment; and an innovative database containing corrective and control data and coefficients to calibrate and correct the instrumental response of the detection equipment, wherein the corrective and control data and coefficients are determined and acquired by the database before the actual analysis of the samples, the sample being prepared with a universal dilution solution to minimize the corresponding matrix effect, the data processing system determining the quantitative data of the target analytes by processing the quantitative data taking account of the corrective and control data and coefficients contained in the database.. .
|Multi-capillary column and high-capacity ionization interface for gc-ms|
A gas chromatograph-mass spectrometer (gc-ms) system includes a multi-capillary gc column coupled to a mass analyzer through an ionization interface. The ionization interface includes an ionization device and an ion guide configured for receiving a high-capacity gas-sample flow from the gc column and transmitting a compressed ion beam to the mass analyzer.
|Exponential scan mode for quadrupole mass spectrometers to generate super-resolved mass spectra|
A novel scanning method of a mass spectrometer apparatus is introduced so as to relate by simple time shifts, rather than time dilations, the component signal (“peak”) from each ion even to an arbitrary reference signal produced by a desired homogeneous population of ions. Such a method and system, as introduced herein, is enabled in a novel fashion by scanning exponentially the rf and dc voltages on a quadrupole mass filter versus time while maintaining the rf and dc in constant proportion to each other.
|Electrokinetically controlled calibrant delivery|
An electrokinetic pump can be used to deliver calibrant (“lock mass”) ions to a mass spectrometer for calibration of a mass spectrometry system. Electrokinetically controlled calibrant delivery can help to eliminate the need for the more cumbersome mechanisms that are often used for ion delivery.
|Method and apparatus for dipolar dc collisional activation of ions transmitted through an electrodynamic multipole device|
A method of operating a quadrupole mass spectrometer is described where one of the stages thereof has a pairs of opposing rods and one of the pair of rods is operated with a zero voltage potential difference therebetween and the other of the pair of opposing rods is operated with a voltage potential difference therebetween. The potential field unbalance causes the analyte ions to deviate from the axial centerline of the stage so as to undergo additional rf heating.
|Atmospheric pressure interface ion source and mass spectrometer|
An api (atmospheric pressure interface) ion source and a mass spectrometer with the same are disclosed. In the disclosed api ion source and the mass spectrometer with the same, the included angle α between the capillary (2) and the mass analyzer (4) of the mass spectrometer (10) is 80°˜150°.
A mass spectrometer is disclosed comprising a device which is operable in a first mode of operation to separate ions temporally according to their ion mobility by applying a continuous axial electric field. The device is also operable in a second mode of operation wherein ions are separated temporally according to their mass to charge ratio by pulsing an applied axial electric field on and off..
|Automatic reconstruction of ms-2 spectra from all ions fragmentation to recognize previously detected compounds|
A method of acquiring and interpreting data using a mass spectrometer system and a local mass spectral library comprises: (a) generating a multiplexed mass spectrum, the multiplexed mass spectrum comprising a superposition of a plurality of product-ion mass spectra comprising a plurality of product-ion types, each product-ion mass spectrum corresponding to fragmentation of a respective precursor-ion type; (b) recognizing a respective set of two or more product-ion types corresponding to each of one or more of the product-ion mass spectra by recognizing correlations between the elution profiles of said two or more product-ion types corresponding to each said respective set; and (c) determining if each recognized set of two or more product-ion types corresponds to a product-ion mass spectrum previously observed using said mass spectrometer system by comparing the m/z ratios of the product ion types to information in at least one entry of the local mass spectral library.. .
|Dual source mass spectrometry system|
A dual source mass spectrometer system (10) is operable in a first mode with an lc source [lc/ms] (12) and in a second mode with a gc source [gc/ms] (18). The gc source inputs into an ion source chamber (22) for delivering the ionized output from the gc source to the mass spectrometer.
|Mass spectrometer and method of adjusting same|
A mass spectrometer and method capable of optimizing the opening time of a collisional cell includes: an ion source (10) for ionizing a sample; a first mass analyzer (30) for selecting first desired ions from the ions generated in the ion source (10); a collisional cell (40) for fragmenting some or all of the first desired ions into product ions; a second mass analyzer (50) for selecting second desired ions from the first desired ions and the product ions; a detector (60) for detecting the second desired ions; and a control section (200) for controlling the collisional cell (40) in such a way that the cell performs a storing operation for storing the first desired ions and the product ions for a given storage time and then performs an opening operation for ejecting the stored ions for a given opening time based on information about settings in an adjustment mode.. .
|Resolution and mass range performance in distance-of-flight mass spectrometry with a multichannel focal-plane camera detector|
A distance-of-flight mass spectrometer (dofms) includes an ion source, a field-free region, an extraction region in which ions are accelerated, and a spatially-selective detector for spatially selectively detecting ions extracted by the extraction region. A method for operating a distance-of-flight mass spectrometer dofms comprises controlling a detection time in such a way as to permit ions with progressively greater mass-to-charge (m/z) ratios to enter the extraction region of the dofms at positions which will permit the ions with progressively greater m/z ratios to enter the detector of the dofms, generating a component mass spectrum at each selected value of detection time, and then assembling a composite mass spectrum by shifting the distance-of-flight axis of each component mass spectrum by a distance corresponding to the change in detection time..
|Methods for generating local mass spectral libraries for interpreting multiplexed mass spectra|
A method of acquiring and compiling data obtained on a mass spectrometer system, comprises: (a) generating a multiplexed mass spectrum comprising a superposed plurality of product-ion mass spectra comprising a plurality of product-ion types, each product-ion mass spectrum corresponding to fragmentation of a respective precursor-ion type, each precursor-ion type and each product ion type having a respective mass-to-charge (m/z) ratio; (b) decomposing the multiplexed product-ion mass spectrum so as to recognize relative abundances of previously-observed product-ion mass spectra within the multiplexed product-ion mass spectrum, the decomposing employing a mass-spectral library having a plurality of entries corresponding to respective product ion mass spectra previously-observed on said mass spectrometer system; (c) recognizing an additional contribution to the multiplexed mass spectrum that is neither attributable to random variation nor to any previously-observed product-ion spectrum; and (d) storing at least one new entry in the mass-spectral library relating to the recognized additional contribution.. .
|Mass spectrometer and method of controlling same|
A mass spectrometer and control method which achieves high-speed scanning while maintaining relatively high sensitivity. The mass spectrometer (1) has: an ion source (2) ; a collisional cell (40) for performing a storing operation for storing at least some of the ions (2) and then performing an ejecting operation for ejecting the stored ions; a second mass analyzer (50) for selecting desired ions; a detector (60) for detecting the desired ions; analog signal processing circuitry (80) for converting a signal from the detector (60) into a voltage; and an a/d converter (90) for sampling and converting the output voltage into a digital signal.
|Interpreting multiplexed tandem mass spectra using local spectral libraries|
A method of acquiring and interpreting data using (i) a mass spectrometer system operated according to a set of operating conditions and (ii) a mass spectral library having a plurality of library entries derived from data previously obtained using said mass spectrometer system operated according to said set of operating conditions comprising: (a) generating, using the mass spectrometer system, a multiplexed mass spectrum comprising a superposition of a plurality of product-ion mass spectra comprising a plurality of product-ion types, each product-ion mass spectrum corresponding to fragmentation of a respective precursor-ion type formed by ionization of the plurality of chemical compounds, each precursor-ion type having a respective precursor-ion mass-to-charge (m/z) ratio and each product ion type having a respective product-ion m/z ratio; and (b) decomposing the multiplexed product-ion mass spectrum, using the mass-spectral library, so as to calculate relative abundances of previously-observed product-ion mass spectra within the multiplexed product-ion mass spectrum.. .
|Mass spectrometer device and method using scanned phase applied potentials in ion guidance|
An ion guide or mass analyser is disclosed comprising a plurality of electrodes having apertures through which ions are transmitted in use. A pseudo-potential barrier is created at the exit of the ion guide or mass analyser.
|Rf power supply for a mass spectrometer|
The present invention provides a radio frequency (rf) power supply in a mass spectrometer. The power supply provides an rf signal to electrodes of a storage device to create a trapping field.
|Use of neutral loss mass to reconstruct ms-2 spectra in all ions fragmentation|
A method is provided for acquiring and interpreting data using a mass spectrometer, said method comprising: (a) generating a multiplexed mass spectrum using the mass spectrometer system, the multiplexed mass spectrum comprising a superposition of a plurality of product-ion mass spectra comprising a plurality of product-ion types having respective product-ion mass-to-charge (m/z) ratios, each product-ion mass spectrum corresponding to fragmentation of a respective precursor-ion type formed by ionization of a chemical compound, each precursor-ion type having a respective precursor-ion mass-to-charge (m/z) ratio and (b) recognizing a set comprising a precursor-ion type and one or more product-ion types corresponding to each of one or more of the product-ion mass spectra by recognizing one or more losses of a respective valid neutral molecule from each said precursor-ion type.. .
|Time of flight mass spectrometer|
A method of determining the mass-to-charge ratios of ions in a sample is disclosed. The method includes determining a data acquisition time, where the data acquisition time is a predetermined fraction of the greatest time of flight.
|Triple quadrupole mass spectrometer|
Elements are arranged so that a straight ion-beam axis extending from an ion source through a first ion lens and a front-stage quadrupole mass filter and a straight ion-beam axis extending through the ion guide in a collision cell and a rear-stage quadrupole mass filter obliquely intersect with each other at a predetermined angle in a space between the front-stage quadrupole mass filter and the collision cell. Metastable helium molecules generated in the ion source may pass through the front-stage quadrupole mass filter but will be removed before reaching the exit of the collision cell.
|Liquid chromatography mass spectrometer device|
The purpose of the present invention is to provide a mass spectrometer with high detection sensitivity to generate fine charged droplets and thereby improve the efficiency of sample ionization, and to reduce large droplets with high ionic strength. The present invention includes: liquid chromatograph separating means that separates a sample solution into components; a sample sprayer that sprays as droplets the sample solution separated and eluted by the liquid chromatograph separating means; ion generating means that charges the droplets and generates ions; a mass spectrometer that receives the ions and performs mass spectrometry on the ions; and a desolvation unit that removes a solvent contained in the charged droplets, wherein the desolvation unit includes a desolvation flow path chamber through which the charged droplets pass, heating means for heating the desolvation flow path chamber, and a helical droplet flow path formed in the desolvation flow path chamber..
|Method to control space charge in a mass spectrometer|
A method for operating a mass spectrometer having an ion trap over a plurality of selected mass-to-charge ranges constituting an overall mass-to-charge range is disclosed. For each of the plurality of selected mass-to-charge ranges the method comprises filling the ion trap with fragmented ions of the selected mass-to-charge ranges, cooling the fragmented ions trapped in the ion trap for a first cooling period, applying an rf voltage and a resolving direct current voltage to the ion trap for eliminating any remaining fragmented ions outside the selected ion mass-to-charge range and retaining ions within the selected ion mass-to-charge range, cooling the retained ions in the ion trap for a second cooling period, and scanning the retained ions out of the ion trap and detecting the ions released therefrom..
A mass spectrometer is disclosed comprising a quadrupole rod set ion trap wherein a potential field is created at the exit of the ion trap which decreases with increasing radius in one radial direction. Ions within the on trap are mass selectively excited in a radial direction.
|Targeted analysis for tandem mass spectrometry|
A tandem mass spectrometer and method are described. Precursor ions are generated in an ion source (10) and an ion injector (21, 23) injects ions towards a downstream ion guide (50, 60) via a single or multi reflection tof device (30) that separates ions into packets in accordance with their m/z.
A collision or fragmentation cell is disclosed comprising a plurality of electrodes wherein a first rf voltage is applied to an upstream group of electrodes and a second different rf voltage is applied to a downstream group of electrodes. The radial confinement of parent ions entering the collision or fragmentation cell is optimised by the first rf voltage applied to the upstream group of electrodes and the radial confinement of daughter or fragment ions produced within the collision or fragmentation cell is optimised by the second different rf voltage applied to the downstream group of electrodes..