Images List Premium Download Classic

Ligase

Ligase-related patent applications - as published by the U.S. Patent and Trademark Office (USPTO).


loading
Ubiquitin variant modulators of scf e3 ligases and their uses
The Governing Council Of The University Of Toronto
November 09, 2017 - N°20170321205

The invention provides ubiquitin variants that specifically bind to scf e3 ligases, and use of these variants to modulate the activity of scf e3 ligases.
Method for nucleic acid analysis directly from an unpurified biological sample
The Governing Council Of The University Of Toronto
October 19, 2017 - N°20170298415

A method is provided for generating single-stranded dna circles from a biological sample. The method comprises the steps of: treating the biological sample with an extractant to release nucleic acids, thereby forming a sample mixture; neutralizing the extractant; denaturing the released nucleic acids to generate single-stranded nucleic acids; and contacting the single-stranded nucleic acids with a ligase that is capable ...
Thermostable blunt-end ligase and methods of use
The Governing Council Of The University Of Toronto
October 19, 2017 - N°20170298338

Fusion proteins having thermostable blunt-end ligase activity are provided. Blunt-end ligases are useful for dna amplification, sequencing, production of recombinant dna and recombinant fusion proteins, and other purposes. These thermostable blunt-end dna ligases are useful in ligation schemes which include, e. G., an incubation at about 60-65° c. Or higher, or as high as about 94° c., or at ...
Ligase Patent Pack
Download + patent application PDFs
Ligase Patent Applications
Download + Ligase-related PDFs
For professional research & prior art discovery
inventor
  • + full patent PDF documents of Ligase-related inventions.
  • Exact USPTO filing data with full-text, images, drawings & claims.
  • Index pages: Table View and Image-Grid View layouts. All images in each PDF.
Chemically modified ligase cofactors, donors and acceptors
Trilink Biotechnologies, Inc.
September 28, 2017 - N°20170275672

Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates ...
Ubiquitin variant modulators of hect e3 ligases and their uses
The Governing Council Of The University Of Toronto
September 28, 2017 - N°20170275341

The invention provides ubiquitin variants that specifically bind to hect e3 ligases, and methods of using these variants to modulate the activity of hect e3 ligases.
Dna-linked enzyme-coupled assays
The Regents Of The University Of California
August 24, 2017 - N°20170240952

Traditional enzyme characterization methods are low-throughput, and therefore limit engineering efforts in synthetic biology and biotechnology. Here we propose a dna-linked enzyme-coupled assay (dlenca) to monitor enzyme reactions in a high-throughput manner. Throughput is improved by removing the need for protein purification and by limiting the need for liquid chromatography mass spectrometry (lcms) product detection by linking enzymatic function to ...
Ligase Patent Pack
Download + patent application PDFs
Ligase Patent Applications
Download + Ligase-related PDFs
For professional research & prior art discovery
inventor
  • + full patent PDF documents of Ligase-related inventions.
  • Exact USPTO filing data with full-text, images, drawings & claims.
  • Index pages: Table View and Image-Grid View layouts. All images in each PDF.
In vitro recombination method
Synthetic Genomics, Inc.
August 17, 2017 - N°20170233764

The present invention relates, e. G., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) dna molecules of interest, wherein the distal region of the first dna molecule and the proximal region of the second dna molecule share a region of sequence identity, comprising contacting the two dna molecules in a reaction mixture with (a) ...
Multiple ligase compositions, systems, and methods
Rgene, Inc.
August 10, 2017 - N°20170226498

Provided herein are compositions, systems, and methods using multiple ligases, wherein at least one of the ligases is an adenylation-deficient atp-dependent ligase or an un-adenylated atp-dependent ligase (e. G., present in an atp free mixture). In certain embodiments, multiple ligases are used to ligate a pre-adenylated double stranded sequence to a non-adenylated double stranded sequence (e. G., the adenylation-deficient atp-dependent ...
Methods and materials for producing 7-carbon monomers
Invista North America S.a.r.l.
August 03, 2017 - N°20170218406

This document describes biochemical pathways for producing pimeloyl-coa using a polypeptide having the enzymatic activity of a hydroperoxide lyase to form non-3-enal and 9-oxononanoate from 9-hydroxyperoxyoctadec-10,12-dienoate. Non-3-enal and 9-oxononanoate can be enzymatically converted to pimeloyl-coa or a salt thereof using one or more polypeptides having the activity of a dehydrogenase, a coa ligase, an isomerase, a reductase, ...
Method for directional cloning
Innovative Cellular Therapeutics Co., Ltd.
August 03, 2017 - N°20170218380

Described herein are techniques for directional cloning an insert dna segments into a target vector. The techniques mix the target vector, the insert dna segment, a restriction enzyme, and a dna ligase to generate a recombinant dna molecule.
Method for identification and enumeration of nucleic acid sequence, expression, copy, or dna methylation changes, ...
Cornell University
July 20, 2017 - N°20170204459

The present invention relates to a method for the highly specific, targeted capture of regions of human genomes and transcriptomes from the blood, i. E. From cell free circulating dna, exosomes, microrna, circulating tumor cells, or total blood cells, to allow for the highly sensitive detection of mutation, expression, copy number, translocation, alternative splicing, and methylation changes using combined nuclease, ...
Oligonucleotides for inducing paternal ube3a expression
Hoffmann-la Roche Inc.
July 06, 2017 - N°20170191064

The present invention relates to oligonucleotides that are capable of inducing expression of ubiquitin-protein ligase e3a (ube3a) from the paternal allele in animal or human neurons. The oligonucleotides target the suppressor of the ube3a paternal allele by hybridization to snhg14 long non-coding rna downstream of snord109b. The present invention further relates to pharmaceutical compositions and methods ...
Amplification and detection of ribonucleic acids
Applied Biosystem, Llc
June 22, 2017 - N°20170175180

Compositions, methods, and kits for detecting one or more species of rna molecules are disclosed. In one embodiment, a first adaptor and a second adaptor are ligated to the rna molecule using a polypeptide comprising double-strand specific rna ligase activity, without an intervening purification step. The ligated product is reverse transcribed, then at least some of the ribonucleosides in the ...
Ligase Patent Pack
Download + patent application PDFs
Ligase Patent Applications
Download + Ligase-related PDFs
For professional research & prior art discovery
inventor
  • + full patent PDF documents of Ligase-related inventions.
  • Exact USPTO filing data with full-text, images, drawings & claims.
  • Index pages: Table View and Image-Grid View layouts. All images in each PDF.
Cationic lipid cordiarimide hybrid compounds and a process for preparation thereof
Council Of Scientific & Industrial Research
May 18, 2017 - N°20170137462

The present invention relates to the cationic lipid cordiaroimide hybrid compounds of formula i. The present invention provides a process for preparation of these compounds is also being elaborated. The compounds described provides anticancerous activity against cell lines including pc-3 (prostate cancer), hepg2 (liver cancer), mcf-7 (breast cancer) and nih-1/3t3 (non cancer cells. The compound was also capable of ...
Single nucleotide detection method
Base4 Innovation Ltd
May 11, 2017 - N°20170130265

A method of sequencing a nucleic acid such as dna or rna is provided. It is characterised by the steps of (1) generating a stream of single nucleoside triphosphates by progressive pyrophosphorolysis of the nucleic acid; (2) producing at least one substantially double-stranded oligonucleotide used probe by reacting in the presence of a polymerase and a ligase at least one of the ...
Tagging and assessing a target sequence
Rhodx, Inc.
May 04, 2017 - N°20170121716

Provided in certain aspects are methods for dynamically adding molecular indexes to nucleic acid, and optionally analyzing the tagged nucleic acid. Also provided in certain aspects are methods for producing a single-stranded nucleic acid molecule from two molecules. The first molecule typically is a single-stranded nucleic acid (ssna) containing a target sequence with optional linked nucleic acid sequences. The second ...
Proteolysis targeting chimera compounds and methods of preparing and using same
Yale University
May 04, 2017 - N°20170121321

The present invention includes novel compounds and methods for preventing or treating diseases associated with and/or caused by overexpression and/or uncontrolled activation of a tyrosine kinase in a subject in need thereof. In certain embodiments, the compounds of the present invention comprise a tyrosine kinase inhibitor, a linker and a ubiquitin ligase binder. The methods of the present ...
Semi-synthetic terephthalic acid via microorganisms that produce muconic acid
Genomatica, Inc.
April 06, 2017 - N°20170096689

The invention provides a non-naturally occurring microbial organism having a muconate pathway having at least one exogenous nucleic acid encoding a muconate pathway enzyme expressed in a sufficient amount to produce muconate. The muconate pathway including an enzyme selected from the group consisting of a beta-ketothiolase, a beta-ketoadipyl-coa hydrolase, a beta-ketoadipyl-coa transferase, a beta-ketoadipyl-coa ligase, a 2-fumarylacetate reductase, a 2-fumarylacetate ...
Recombinant rna production
Universitat Politècnica De València
March 30, 2017 - N°20170088871

This invention relates to the production of rna by co-expressing a trna ligase and a chimeric rna molecule comprising a target rna and a plant viroid scaffold, such as eggplant latent viroid, in a host cell. This co-expression causes the production of large amounts of the chimeric rna molecule in the host cells and may be useful for example in ...
Compounds and methods for the targeted degradation of bromodomain-containing proteins
Yale University
March 09, 2017 - N°20170065719

The present invention relates to bifunctional compounds, which find utility as modulators of targeted ubiquitination, especially inhibitors of a variety of polypeptides and other proteins which are degraded and/or otherwise inhibited by bifunctional compounds according to the present invention. In particular, the present invention is directed to compounds, which contain on one end a vhl ligand which binds to ...
Library construction using y-adapters and vanishing restriction sites
Centrillion Technology Holdings Corporation
February 16, 2017 - N°20170044600

The present disclosure describes systems and methods to construct genomic dna fragments library by stretching genomic dna over an adapter-modified chip; using an enzyme cocktail comprising a first restriction enzyme, a ligase, and a second restriction enzyme to cut the genomic dna into fragments and ligate the fragments to the adapters on the chip; and amplifying thus-obtained dna by pcr ...
Loading