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Ligase

Ligase-related patent applications - as published by the U.S. Patent and Trademark Office (USPTO).


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Method of designing addressable array suitable for detection of nucleic acid sequence differences using ligase ...
Cornell Research Foundation, Inc.
January 04, 2018 - N°20180002744

The present invention is directed to a method of designing a plurality of capture oligonucleotide probes for use on a support to which complementary oligonucleotide probes will hybridize with little mismatch, where the plural capture oligonucleotide probes have melting temperatures within a narrow range. The present invention further relates to an oligonucleotide array comprising of a support with the plurality ...
Methods of constructing a circular template and detecting dna molecules
Personal Genomics, Inc.
January 04, 2018 - N°20180002731

A method of constructing a circular template includes preparing a partially double stranded linear dna molecule, and incubating the partially double stranded linear dna molecule with a ligase capable of intra-molecular ligation of single stranded dna molecules to generate a partially double stranded circular dna molecule. A method of detecting dna molecules includes the following steps. Target dna molecules are ...
Modulation of ube3a-ats expression
Ionis Pharmaceuticals, Inc.
December 21, 2017 - N°20170362592

Certain embodiments are directed to methods and compounds for inhibiting ube3a-ats, the endogenous antisense transcript of ubiquitin protein ligase e3a (ube3a). Such methods and compounds are useful for. Several embodiments provided herein relate to the discovery that antisense compounds targeting ube3a-ats induce paternal expression of ube3a. Several embodiments are drawn to methods and compounds for ...
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Pharmaceuticals and methods for treating hypoxia and screening methods therefor
Dana Farber Cancer Institute, Inc.
December 07, 2017 - N°20170350891

Light-generating fusion proteins having a ligand binding site and a light-generating polypeptide moiety and their use as diagnostics, in drug screening and discovery, and as therapeutics, are disclosed. The light-generating fusion protein has a feature where the bioluminescence of the polypeptide moiety changes upon binding of a ligand at the ligand binding site. The ligand may be, for example, an ...
Strand displacement stop (sds) ligation
Lexogen Gmbh
November 30, 2017 - N°20170342409

A method of ligating dna molecules, wherein the dna molecules are in a hybrid with an rna molecule, including the steps of providing dna molecules that are in a rna:dna hybrid with an rna molecule, and ligating the dna molecules to each other with a double strand specific ligase
Compounds and methods for the targeted degradation of androgen receptor
Arvinas, Inc.
November 16, 2017 - N°20170327469

The present disclosure relates to bifunctional compounds, which find utility to degrade (and inhibit) androgen receptor. In particular, the present invention is directed to compounds, which contain on one end a vhl ligand which binds to the ubiquitin ligase and on the other end a moiety which binds androgen receptor such that androgen receptor is placed in proximity to the ...
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Method for nucleic acid analysis directly from an unpurified biological sample
October 19, 2017 - N°20170298415

A method is provided for generating single-stranded dna circles from a biological sample. The method comprises the steps of: treating the biological sample with an extractant to release nucleic acids, thereby forming a sample mixture; neutralizing the extractant; denaturing the released nucleic acids to generate single-stranded nucleic acids; and contacting the single-stranded nucleic acids with a ligase that is capable ...
Thermostable blunt-end ligase and methods of use
October 19, 2017 - N°20170298338

Fusion proteins having thermostable blunt-end ligase activity are provided. Blunt-end ligases are useful for dna amplification, sequencing, production of recombinant dna and recombinant fusion proteins, and other purposes. These thermostable blunt-end dna ligases are useful in ligation schemes which include, e. G., an incubation at about 60-65° c. Or higher, or as high as about 94° c., or at ...
Method for breaking nucleic acid and adding adaptor by means of transposase, and reagent
Bgi Shenzhen Co., Limited
October 12, 2017 - N°20170292153

Provided are a method for breaking a nucleic acid and adding an adaptor by means of a transposase, and a reagent. The method comprises the following steps: conducting random breaking of a nucleic acid by using a transposase-embedded complex, wherein the transposase-embedded complex comprises a transposase and a first adaptor comprising a transposase identification sequence, and two ends of the ...
Chemically modified ligase cofactors, donors and acceptors
Trilink Biotechnologies, Inc.
September 28, 2017 - N°20170275672

Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates ...
Ubiquitin variant modulators of hect e3 ligases and their uses
The Governing Council Of The University Of Toronto
September 28, 2017 - N°20170275341

The invention provides ubiquitin variants that specifically bind to hect e3 ligases, and methods of using these variants to modulate the activity of hect e3 ligases.
Dna-linked enzyme-coupled assays
The Regents Of The University Of California
August 24, 2017 - N°20170240952

Traditional enzyme characterization methods are low-throughput, and therefore limit engineering efforts in synthetic biology and biotechnology. Here we propose a dna-linked enzyme-coupled assay (dlenca) to monitor enzyme reactions in a high-throughput manner. Throughput is improved by removing the need for protein purification and by limiting the need for liquid chromatography mass spectrometry (lcms) product detection by linking enzymatic function to ...
Modified lipopolysaccharide glycoform and method of use
The Trustees Of Princeton University
August 24, 2017 - N°20170239350

The present disclosure generally relates to genetic engineering of bacteria. More particularly, the present disclosure describes genetic engineering of e. Coli to create mutant o-antigen ligase, as well as novel lipopolysaccharide molecules resulting from that genetic engineering. Methods for using those novel molecules are also described.
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Multiple ligase compositions, systems, and methods
Rgene, Inc.
August 10, 2017 - N°20170226498

Provided herein are compositions, systems, and methods using multiple ligases, wherein at least one of the ligases is an adenylation-deficient atp-dependent ligase or an un-adenylated atp-dependent ligase (e. G., present in an atp free mixture). In certain embodiments, multiple ligases are used to ligate a pre-adenylated double stranded sequence to a non-adenylated double stranded sequence (e. G., the adenylation-deficient atp-dependent ...
Methods and materials for producing 7-carbon monomers
Invista North America S.a.r.l.
August 03, 2017 - N°20170218406

This document describes biochemical pathways for producing pimeloyl-coa using a polypeptide having the enzymatic activity of a hydroperoxide lyase to form non-3-enal and 9-oxononanoate from 9-hydroxyperoxyoctadec-10,12-dienoate. Non-3-enal and 9-oxononanoate can be enzymatically converted to pimeloyl-coa or a salt thereof using one or more polypeptides having the activity of a dehydrogenase, a coa ligase, an isomerase, a reductase, ...
Method for directional cloning
Innovative Cellular Therapeutics Co., Ltd.
August 03, 2017 - N°20170218380

Described herein are techniques for directional cloning an insert dna segments into a target vector. The techniques mix the target vector, the insert dna segment, a restriction enzyme, and a dna ligase to generate a recombinant dna molecule.
Method for identification and enumeration of nucleic acid sequence, expression, copy, or dna methylation changes, ...
Cornell University
July 20, 2017 - N°20170204459

The present invention relates to a method for the highly specific, targeted capture of regions of human genomes and transcriptomes from the blood, i. E. From cell free circulating dna, exosomes, microrna, circulating tumor cells, or total blood cells, to allow for the highly sensitive detection of mutation, expression, copy number, translocation, alternative splicing, and methylation changes using combined nuclease, ...
Oligonucleotides for inducing paternal ube3a expression
Hoffmann-la Roche Inc.
July 06, 2017 - N°20170191064

The present invention relates to oligonucleotides that are capable of inducing expression of ubiquitin-protein ligase e3a (ube3a) from the paternal allele in animal or human neurons. The oligonucleotides target the suppressor of the ube3a paternal allele by hybridization to snhg14 long non-coding rna downstream of snord109b. The present invention further relates to pharmaceutical compositions and methods ...
Amplification and detection of ribonucleic acids
Applied Biosystem, Llc
June 22, 2017 - N°20170175180

Compositions, methods, and kits for detecting one or more species of rna molecules are disclosed. In one embodiment, a first adaptor and a second adaptor are ligated to the rna molecule using a polypeptide comprising double-strand specific rna ligase activity, without an intervening purification step. The ligated product is reverse transcribed, then at least some of the ribonucleosides in the ...
In vitro method for fast scarless dna assembly using thermostable exonucleases and ligase
Jiangnan University
June 22, 2017 - N°20170175156

The present invention provides a method for scarless in vitro dna assembly using thermostable exonucleases and ligase, which relates to the field of genetic engineering. The present invention provides a fast method for assembling dna subfragments with homologous ends, which employs thermostable polymerases and ligase in a thermal cycle of denaturation, annealing, digestion and ligation. After denaturation, dna subfragments are ...
Cationic lipid cordiarimide hybrid compounds and a process for preparation thereof
Council Of Scientific & Industrial Research
May 18, 2017 - N°20170137462

The present invention relates to the cationic lipid cordiaroimide hybrid compounds of formula i. The present invention provides a process for preparation of these compounds is also being elaborated. The compounds described provides anticancerous activity against cell lines including pc-3 (prostate cancer), hepg2 (liver cancer), mcf-7 (breast cancer) and nih-1/3t3 (non cancer cells. The compound was also capable of ...
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