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Human Embryonic Stem Cell patents

      

This page is updated frequently with new Human Embryonic Stem Cell-related patent applications.




 Differentiation of human embryonic stem cells patent thumbnailDifferentiation of human embryonic stem cells
The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells, wherein greater than 85% of the cells in the population express markers characteristic of the definitive endoderm lineage..
Janssen Biotech, Inc.


 Differentiation of human embryonic stem cells into pancreatic endocrine cells patent thumbnailDifferentiation of human embryonic stem cells into pancreatic endocrine cells
The present invention provides methods to promote differentiation of pancreatic endoderm cells to pancreatic endocrine rich clusters and to enhance insulin expression in hormone-expressing cells.. .
Janssen Biotech, Inc.


 Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells patent thumbnailMethods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
The present disclosure relates to compositions and methods comprising cell surface markers for hes-derived cells, in particular, endoderm lineage cells including pancreatic endoderm-type cells, derived from hes cells.. .
Viacyte, Inc.


 Differentiation of human embryonic stem cells patent thumbnailDifferentiation of human embryonic stem cells
The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells, wherein greater than 80% of the cells in the population express markers characteristic of the definitive endoderm lineage..
Janssen Biotech, Inc.


 Method for inducing pluripotency in a hematopoietic cell patent thumbnailMethod for inducing pluripotency in a hematopoietic cell
There is provided a method of inducing pluripotency in a hematopoietic cell. The method comprises providing a blood sample that has been collected from a subject in the absence of any polysaccharide preparation used for separating blood components.
Agency For Science, Technology And Research


 Composition for preventing and treating acetaminophen inducing hepatotoxicity containing tnp(n2-(m-trifluorobenzyl), n6-(p-nitrobenzyl)purine) as an effective ingredient patent thumbnailComposition for preventing and treating acetaminophen inducing hepatotoxicity containing tnp(n2-(m-trifluorobenzyl), n6-(p-nitrobenzyl)purine) as an effective ingredient
The present invention relates to a composition for the prevention and treatment of liver toxicity originated from acetaminophen comprising tnp (n2-(m-trifluorobenzyl), n6-(p-nitrobenzyl)purine) as an active ingredient. The present inventors confirmed that tnp known as a 5-inosito pyrophosphate inhibitor suppressed apoptosis caused by acetaminophen in human embryonic stem cell-derived liver cells, mouse liver cells, and human hepatoma cell lines, up-regulated glutathione converted in liver cells, and inhibited jnk phosphorylation that is a kind of response against stress increased by acetaminophen.
Korea Advanced Institute Of Science And Technology


 Methods and compositions for producing stem cell derived dopaminergic cells for use in treatment of neurodegenerative diseases patent thumbnailMethods and compositions for producing stem cell derived dopaminergic cells for use in treatment of neurodegenerative diseases
The present disclosure relates to methods for producing dopaminergic cells and evaluating their functionality. When pluripotent human embryonic stem cells are cultured on plates coated with laminin-111, laminin-121, laminin-521, laminin-421, or laminin-511 in cell culture medium containing a gsk3 inhibitor and a tgf-β inhibitor as well as timely administered fibroblast growth factor, desired neural cells are produced at far higher rates.
Biolamina Ab


 Media for culturing stem cells patent thumbnailMedia for culturing stem cells
Well-defined, xeno-free culture media which comprise a tgf-beta isoform or the chimera formed between il6 and the soluble il6 receptor (il6ril6), which are capable of maintaining stem cells, and particularly, human embryonic stem cells, in an undifferentiated state are provided. Also provided are cell cultures comprising the culture media and the stem cells and methods of expanding and deriving embryonic stem cells in such well-defined, xeno-free culture media.
Technion Research & Development Foundation Limited


 Medium comprising transforming growth factor beta 1 and basic fibroblast growth factor patent thumbnailMedium comprising transforming growth factor beta 1 and basic fibroblast growth factor
The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.. .
Technion Research & Development Foundation Limited


 Compositions and methods for promoting the generation of pdx1+ pancreatic cells patent thumbnailCompositions and methods for promoting the generation of pdx1+ pancreatic cells
Certain embodiments disclosed herein are directed to a method of producing pancreatic cells or pancreatic cell precursors by exposing human embryonic stem cells to an effective amount of at least one compound listed in table i to differentiate the human embryonic stem cells into the pancreatic cells or the pancreatic cell precursors. Kits and pancreatic cell lines produced using the methods are also described..
The General Hospital Corporation


Substantially pure human retinal progenitor, forebrain progenitor, and retinal pigment epithelium cell cultures and methods of making the same

Methods for producing substantially pure cultures of human neural retinal progenitor cells, forebrain progenitor cells, and retinal pigment epithelial cells are disclosed. In addition, the successful differentiation of human embryonic stem cells and human induced pluripotent stem cells through the major developmental stages of human retinogenesis is disclosed..
Wisconsin Alumni Research Foundation

Method of nociceptor differentiation of human embryonic stem cells and uses thereof

The present invention relates to the field of stem cell biology, in particular the linage specific differentiation of pluripotent or multipotent stem cells, which can include, but is not limited to, human embryonic stem cells (hesc), human induced pluripotent stem cells (hipsc), somatic stem cells, cancer stem cells, or any other cell capable of lineage specific differentiation. Specifically described are methods to direct the lineage specific differentiation of hesc and/or hipsc to nociceptors (i.e.
Memorial Sloan Kettering Cancer Center

Patient-specific stem cell lines derived from human parthenogenetic blastocysts

Methods are disclosed for generating hla homozygous parthenogenetic human stem cell (hpsc-hhom) lines from both hla homozygous and hla heterozygous donors. These hpsc-hhom lines demonstrate typical human embryonic stem cell morphology, expressing appropriate stem cell markers and possessing high levels of alkaline phosphatase and telomerase activity.

Composition for preventing and treating liver fibrosis or liver cirrhosis, containing, as active ingredient, mesenchymal stem cells derived from human embryonic stem cells

The present invention relates to a composition for preventing and/or treating liver fibrosis or liver cirrhosis, which contains, as an active ingredient, mesenchymal stem cells derived from human embryonic stem cells. In the present invention, it was confirmed that mesenchymal stem cells derived from human embryonic stem cells have a preventive effect against liver fibrosis.
Seoul National University Hospital

Method for differentiation of pluripotent stem cells into multi-competent renal precursors

This application relates to a method for differentiating pluripotent stem cells (pscs) into multi-competent renal precursor cells expressing six2. These renal precursor cells are able to differentiate into fully functional and fully differentiated podocytes.
Hoffmann-la Roche Inc.

Parthenogenic activation of human oocytes for the production of human embryonic stem cells

Methods of producing human stem cells are disclosed for parthenogenetically activating human oocytes by manipulation of o2 tension, including manipulation of ca2+ under high o2 tension and contacting oocytes with serine threonine kinase inhibitors under low o2 tension, isolating inner cell masses (icms) from the activated oocytes, and culturing the cells of the isolated icms under high o2 tension. Moreover, methods are described for the production of stems cells from activated oocytes in the absence of non-human animal products, including the use of human feeder cells/products for culturing icm/stem cells.
International Stem Cell Corporation

Technologies, methods, and products of small molecule directed tissue and organ regeneration from human pluripotent stem cells

Pluripotent human embryonic stem cells (hescs) hold great potential for restoring tissue and organ function, which has been hindered by inefficiency and instability of generating desired cell types through multi-lineage differentiation. This instant invention is based on the discovery that pluripotent hescs maintained under defined culture conditions can be uniformly converted into a specific lineage by small molecule induction.
San Diego Regenerative Medicine Institute

Multipotent neural cells

The inventions disclosed herein are based on the identification of novel cell populations derived from human embryonic stem cells and other pluripotent cells. The inventive cell populations may be used for cell therapies for the treatment of various neurological diseases and as substrates in pharmacological assays..
The Mclean Hospital Corporation

Method of deriving mature hepatocytes from human embryonic stem cells

A related method of maintaining the functional hepatic enzyme activity of primary hepatocytes over time is also disclosed. The method includes the step of transferring an external vector comprising the dna sequence coding for a microrna having the seed sequence of the microrna mir-122 into one or more cultured primary hepatocytes..

Differentiation of human embryonic stem cells

The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce cells expressing markers characteristic of the pancreatic endocrine lineage that co-express nkx6.1 and insulin and minimal amounts of glucagon..
Janssen Biotech, Inc.

Somatic cell nuclear transfer methods

The present invention provides methods for making reconstructed diploid human oocytes comprising the diploid genome of a human somatic cell, and also methods for making human nuclear transfer embryos, human embryonic stem cells, and human differentiated cells therefrom. The present invention also provides reconstructed human oocytes, human nuclear transfer embryos, human embryonic stem cells, and differentiated cells made using such methods, as well as compositions and kits useful in performing such methods..

Methods for production of platelets from pluripotent stem cells and compositions thereof

Methods for production of platelets from pluripotent stem cells, such as human embryonic stem cells (hescs) and induced pluripotent stem cells (ipscs) are provided. These methods may be performed without forming embryoid bodies or clusters of pluripotent stem cells, and may be performed without the use of stromal inducer cells.
Advanced Cell Technology, Inc.

Differentiation of human embryonic stem cells into single hormonal insulin positive cells

The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides methods to produce a population of cells, wherein greater than 10% of the cells in the population express markers characteristic of single hormonal pancreatic beta cells..
Janssen Biotech, Inc.

Method for differentiation of pluripotent stem cells into cardiomyocytes

This application relates to a method for differentiating pluripotent stem cells (pscs) into cardiomyocytes. Moreover this application relates to a method for differentiating human embryonic stem cells (hescs) and induced pluripotent stem cells (ipscs) into defined cardiomyocytes based on linked steps of chemically defined medium inductions..
Hoffmann-la Roche Inc.

Modalities for the treatment of degenerative diseases of the retina

This invention relates to methods for improved cell-based therapies for retinal degeneration and for differentiating human embryonic stem cells and human embryo-derived into retinal pigment epithelium (rpe) cells and other retinal progenitor cells.. .
Ocata Therapeutics, Inc.

Methods for production of platelets from pluripotent stem cells and compositions thereof

Methods for production of platelets from pluripotent stem cells, such as human embryonic stem cells (hescs) and induced pluripotent stem cells (ipscs) are provided. These methods may be performed without forming embryoid bodies or clusters of pluripotent stem cells, and may be performed without the use of stromal inducer cells.
Ocata Therapeutics, Inc.

Suspension culture of human embryonic stem cells

This disclosure provides an improved system for culturing human embryonic stem cells. The cells are cultured in suspension so as to maximize the production capacity of the culture environment.
Asterias Biotherapeutics, Inc.

Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells

The present disclosure relates to compositions and methods comprising cell surface markers for hes-derived cells, in particular, endoderm lineage cells including pancreatic endoderm-type cells, derived from hes cells.. .
Viacyte, Inc.

Methods of producing rpe cells

The present disclosure relates to the use of laminin-521 in obtaining retinal pigment epithelium (rpe) cells. Pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in totally defined and xeno-free conditions.
Biolamina Ab

Novel pseudotyped lentiviral particles and their use in the in vitro targeted transduction of undifferentiated pluripotent human embryonic stem cells and induced pluripotent stem cells

The inventors developed novel pseudotyped lentiviral vector particles comprising a morbillivirus fusion (f) protein and a mutated hemagglutinin (h) protein of the measles virus (mev) or the edmonton strain of the measles virus (mevedm), wherein the cytoplasmic portions of the f and the h protein are truncated, and wherein the amino acids necessary for receptor recognition in the h protein are mutated that it does not interact with cd46, slam and/or nectin-4 and further has a single chain antibody to a cell surface marker of hescs and ipscs at its ectodomain. In this invention, the single chain variable fragment (scfv) anti-cell surface marker coding sequence of the single chain antibody is fused to the coding sequence at the ectodomain of the h protein, wherein the single chain antibody is selected from the group consisting of cd30, epcam (cd326), cd9, thy-1 (cd90), ssea-3, ssea-4, tra-1-60 or tra-1-81.

Differentiation of human embryonic stem cells

The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides an improved method for the formation of pancreatic endoderm, pancreatic hormone expressing cells and pancreatic hormone secreting cells.

Differentiation of human embryonic stem cells

The present invention provides methods to promote the differentiation of pluripotent stem cells and the products related to or resulting from such methods. In particular, the present invention provides an improved method for the formation of pancreatic hormone expressing cells and pancreatic hormone secreting cells.
Lifescan, Inc.

Methods of expanding embryonic stem cells in a suspension culture

A method of expanding and maintaining human embryonic stem cells (escs) in an undifferentiated state by culturing the escs in a suspension culture under culturing conditions devoid of substrate adherence is provided. Also provided are a method of deriving esc lines in the suspension culture and methods of generating lineage-specific cells from escs which were expanded in the suspension culture of the present invention..
Technion Research & Development Foundation Limited

Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state

Provided are novel serum-free culture media which comprise basic fibroblast growth factor (bfgf), transforming growth factor beta-3 and ascorbic acid at a concentration of at least about 50 microgram/ml; ascorbic acid at a concentration range of about 400-600 microgram/ml, bfgf at a concentration range of about 50-200 ng/ml, xeno-free serum replacement and a lipid mixture; the il6ril6 chimera at a concentration range of about 50-200 picogram per milliliter (pg/ml); or leukemia inhibitory factor (lif) at a concentration of at least 2000 units/ml; cell cultures comprising same with pluripotent stem cells such as human embryonic stem cells and induced pluripotent stem (ips) cells, and methods of using same for expanding pluripotent stem cells in an undifferentiated state using two-dimensional or three-dimensional culture systems; and methods of expanding ips cells in a suspension culture devoid of substrate adherence and cell encapsulation.. .
Technion Research & Development Foundation Limited

Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof

The present invention relates to methods of generating and expanding hitman embryonic stem eel! derived mesenchymal-like stem/siromal cells. These hes-mscs are characterized at least in part by the low level of expression of il-6.
Imstem Biotechnology, Inc.

Method of deriving mesenchymal stem cells

We describe a method of obtaining a cell culture, the method comprising providing a cell obtained by dispersing a human embryonic stem cell (hesc) colony, or a descendent thereof, and propagating the cell in the absence of a feeder cell layer in a serum free medium comprising fgf2 and optionally pdgf ab. Preferably, the human embryonic stem cell (hesc) colony is dispersed with a dispersing agent which is trypsin..
Agency For Science, Technology And Research

Differentiation of human embryonic stem cells

The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides a method to increase the expression of markers associated with the pancreatic endocrine lineage..
Janssen Biotech, Inc.

Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof

The disclosure provided herein relates generally to mesenchymal-like stem cells “hes-t-misc” or “t-msc” and the method of producing the stem cells. The method comprises culturing embryonic stem cells under conditions that the embryonic stem cells develop through an intermediate differentiation of trophoblasts, and culturing the differentiated trophoblasts to hes-t-msc or t-msc, t-msc derived cells and cell lineages “t-msc-dl” are also described.
Imstem Biotechnology, Inc.

Novel low-molecular-compound for improving production, maintenance and proliferation of pluripotent stem cells, composition comprising the same, and culture method

According to the present invention, when the novel low-molecular-weight compound rsc-133 is added in a culture process for producing reprogrammed pluripotent stem cells from human differentiated cells, it can increase the efficiency of reprogramming and can significantly reduce the time required for the induction of reprogramming. Particularly, the novel compound rsc-133 can substitute for c-myc acting as both a reprogramming factor and an oncogenic factor, and it can effectively increase the efficiency of reprogramming in both normal oxygen culture conditions and hypoxic culture conditions.
Korea Reasearch Institute Of Bioscience And Biotechnology

Method for preparing endocrine aggregate of insulin-producing beta cells from human pluripotent stem cells

The present invention prepared insulin-producing endocrine cells by inducing the differentiation of human embryonic stem cells or human induced pluripotent stem cells into definitive endoderm (de), pancreatic endoderm (pe), endocrine progenitors (ep), and endocrine cells (ec) stepwise in that order. Particularly, the present invention established the conditions for the formation of an insulin producing endocrine aggregate (ea) from the endocrine cells.
Korea Advanced Institute Of Science And Technology

Differentiation of human embryonic stem cells

The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides an improved method for the formation of pancreatic endoderm, pancreatic hormone expressing cells and pancreatic hormone secreting cells.
Lifescan, Inc.

Method of in vitro differentiation of motor neuron progenitors (mnps) from human induced pluripotent stem cells and cryopreservation of mnps

Methods are disclosed for the initiation and differentiation of human embryonic stem cells (hescs) and induced pluripotent stem cells (ipscs) into motor neuron progenitor cells (mnps). Methods are also disclosed for the cryopreservation of mnps.
Lonza Walkersville, Inc.

Methods for culture and production of single cell populations of human embryonic stem cells (hescs)

We used accutase®, a commercially available cell detachment solution, for single cell propagation of pluripotent hescs. Unlike trypsin dissociation, accutase® treatment does not significantly affect the plating efficiency of hesc dissociation into single cells.
Burnham Institute For Medical Research

Method of generating natural killer cells and dendritic cells from human embryonic stem cell-derived hemangioblasts

This invention provides methods of generating natural killer (nk) cells and dendritic cells (dcs). The methods utilize human hemangioblasts as intermediate cells to generate the nk cells and dcs.
Stem Cell & Regenerative Medicine International, Inc.

Vessel for culturing human es cells

Provided are a culture vessel with which an embryonic body can be formed efficiently from human embryonic stem cells, and a method for culturing human embryonic stem cells using the vessel. There is provided a vessel for culturing human embryonic stem cells, the culture vessel having two or more wells (1), wherein each of the wells (1) has a tubular body (2) and a funnel-shaped bottom (3) provided at one end of the body (2), the bottom (3) being a concave curved surface at the center (4) of the bottom (3) and the bottom (3) having an opening angle (θ) in range of 60 to 100°.
Sumitomo Bakelite Co., Ltd.

Compositions and methods for preventing allogeneic immune rejection

The present invention provides methods for preventing the allogeneic immune rejection of allogeneic cells, such as cells derived from human embryonic stem cells (hescs), without suppressing the entire immune system. Also provided a vector containing a ctla4-ig and pd-l1 expression cassette, and compositions containing a ctla4-ig and pd-l1 for use in preventing allogeneic immune rejection of allogeneic cells..
The Regents Of The University Of California

Medium comprising transforming growth factor beta 1 and basic fibroblast growth factor

The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.. .
Technion Research & Development Foundation Limited

Methods of producing rpe cells and compositions of rpe cells

The present invention provides improved methods for producing rpe cells from human embryonic stem cells or from other human pluripotent stem cells. The invention also relates to human retinal pigmented epithelial cells derived from human embryonic stem cells or other human multipotent or pluripotent stem cells.
Advanced Cell Technology, Inc.

Ocular therapeutics using embryonic stem cell microvesicles

Disclosed is a therapeutic composition comprising human embryonic stem cell-derived micro vesicles, and methods of their use, including treatment of eye pathologies and of obtaining retinal neural cells and retinal stem cells.. .
The Regents Of The University Of California

Metabolite for improving production, maintenance and proliferation of pluripotent stem cells, composition comprising the same, and culturing pluripotent stem cell using the same

According to the present invention, when nicotinamide is added in a culture process for producing pluripotent stem cells from human differentiated cells, it can increase the efficiency of reprogramming and can significantly reduce the time required for induction of reprogramming. It was verified that nicotinamide inhibits the induction of senescence and oxidative stress in the reprogramming process and increases cell proliferation and mitochondrial activity to effectively improve culture conditions for induction of reprogramming.
Korea Research Institute Of Bioscience And Biotech

Primate embryonic stem cells

A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: ssea-1 (−); ssea-4 (+); tra-1-60 (+); tra-1-81 (+); and alkaline phosphatase (+).
Wisconsin Alumni Research Foundation



Human Embryonic Stem Cell topics:
  • Embryonic Stem Cell
  • Human Embryonic Stem Cell
  • Stem Cells
  • Differentiation
  • Culture Media
  • Amino Acid
  • Pancreatic
  • Hepatocyte
  • Type I Diabetes
  • Dna Sequence
  • Supplements
  • Growth Factor
  • Endothelial Cell
  • Transplant
  • Endothelial


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    This listing is a sample listing of patent applications related to Human Embryonic Stem Cell for is only meant as a recent sample of applications filed, not a comprehensive history. There may be associated servicemarks and trademarks related to these patents. Please check with patent attorney if you need further assistance or plan to use for business purposes. This patent data is also published to the public by the USPTO and available for free on their website. Note that there may be alternative spellings for Human Embryonic Stem Cell with additional patents listed. Browse our RSS directory or Search for other possible listings.


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