This page is updated frequently with new Fluorescent Dye-related patent applications.
|Nucleic acid amplification method|
The invention relates to a method for the simultaneous detection of at least four nucleic acids labeled with a covalently attached dye in a sample comprising the step of detecting the fluorescence emission of said fluorescent dyes upon excitation, wherein the at least four dyes attached to said nucleic acids are selected from the groups of i) 6-fam or 5-fam or a blend thereof covalently attached to a first nucleic acid, ii) dy-530, hex or atto 532 or a blend thereof covalently attached to a second nucleic acid, iii) att0550 or dy-555 or a blend thereof covalently attached to a third nucleic acid, iv) att0565, dy510-xl or rox or a blend thereof covalently attached to a fourth nucleic acid, v) dy632 or dy520-xl or a blend thereof covalently attached to a fifth nucleic acid and, vi) chromeo 494 attached to a sixth nucleic acid, wherein chromeo 494 must be one of the four selected.. .
|Super-resolution microscopy methods and systems enhanced by dielectric microspheres or microcylinders used in combination with metallic nanostructures|
Methods and systems for the super-resolution imaging can make visible strongly subwavelength feature sizes (even below 100 nm) in the optical images of biomedical or any nanoscale structures. The main application of the proposed methods and systems is related to label-free imaging where biological or other objects are not stained with fluorescent dye molecules or with fluorophores.
An object is to provide an immunoassay method requiring neither a solid-phase immobilization step nor a washing step, enabling quick and simple quantitative measurement of a target substance in a liquid phase and capable of visualizing an antigen. Such an object is attained by measuring the concentration of a target antigen present in a test substance by sequentially performing a step (a) of bringing an antibody light-chain variable region polypeptide and an antibody heavy-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase; or bringing an antibody heavy-chain variable region polypeptide and an antibody light-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase; a step (b) of measuring the fluorescence intensity of the fluorescent dye; and a step (c) of computationally obtaining the level of the antigen contained in the test substance with reference to a positive correlation between the concentration of the antigen in a liquid phase and the fluorescence intensity of the fluorescent dye..
Ushio Denki Kabushiki Kaisha
|Fluorometric immunoassay for detection of anti-dsdna antibodies|
The present invention relates to the field of immunological methods, more precisely to the field of detection methods for antibodies against double-stranded dna (dsdna) for diagnostics of chronic autoimmune diseases, such as systemic lupus erythematosus (sle). The fluorometric immunoassay method for detection of anti-dsdna solves the technical problem of designing a method for detection of the aforesaid antibodies, which would be faster, cheaper and less toxic as the standard farr-ria method, but would have the same diagnostic specificity (which is 100%) and improved diagnostic sensitivity (for 3%).
Univerzitetni Klinicni Center Ljubljana
|Bright fluorochromes based on multimerization of fluorescent dyes|
The fluorescent dyes can be conjugated to a biomolecule and used for flow cytometry and/or by fluorescence microscopy.. .
|Rapid protein labeling and analysis|
The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein.
Life Technologies Corporation
|Phosphole compound and fluorescent dye containing the same|
The following formula is an example of a phosphole compound of the present invention. The phosphole compound of the present invention has the characteristic of maintaining a high fluorescence quantum yield in a wide range of solvents from low-polarity solvents to high-polarity solvents, as well as the characteristic in which the fluorescence wavelength shifts to the longer wavelength side as the polarity of the solvent increases.
National University Corporation Nagoya University
|Organic electroluminescent device and manufacture thereof|
The present invention discloses an organic electroluminescent device and a manufacturing method thereof. The host material of the light-emitting layer of the organic electroluminescent device is material in which the triplet state energy level of the ct excited state is higher than that of the n-π excited state by 0 to 0.3 ev; or the triplet state of the host material of the light-emitting layer is higher than that of the n-π excited state by more than 1.0 ev; in addition, the difference in energy level between the second triplet state of the n-π state and the first singlet state of the ct excited state is −0.1 to 0.1 ev; and the luminescent dye is a fluorescent dye.
|Carboxy x rhodamine analogs|
The present invention provides novel fluorescent dyes and kits containing the same, which are useful for labeling a wide variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis and/or treatment of disease conditions..
|Solid-state white light generating lighting arrangements including photoluminescence wavelength conversion components|
A white light photoluminescence wavelength conversion component comprises at least one blue light excitable green to yellow light (510 nm to 570 nm) emitting yttrium aluminum garnet (yag) type phosphor material and at least one blue light excitable orange to red light (585 nm to 670 nm) emitting organic fluorescent dye.. .
Solar energy funneling using thermoplastics for algae and cyanobacteria growth
Disclosed is a wavelength conversion material for use in a photo-bioreactor for growing phototrophic organisms. The wavelength conversion material includes an organic fluorescent dye and a polymeric matrix, wherein the organic fluorescent dye is solubilized in the polymeric matrix.
Sabic Global Technologies B.v.
Fluorescent dyes and related methods
Fluorescent dyes with affinity for nucleic acids and related methods are provided. Dielectric or semiconducting films including fluorescent dyes with affinity for nucleic acids and related methods are also provided.
University Of Washington Through Its Center For Commercialization
Fluorescent dyes and methods of use thereof
One method embodiment includes contacting reactive group z of the fluorescent dye compound with the target molecule such that reactive group z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair.
Method for detecting pathogens using microbeads conjugated to biorecognition molecules
A method and system are provided for the simultaneous detection and identification of multiple pathogens in a patient sample. The sample is combined with microbeads, which have been injected with quantum dots or fluorescent dye and conjugated to pathogen-specific biorecognition molecules, such as antibodies and oligonucleotides.
Method and system for multiplexed time-resolved fluorescence detection
The present invention is directed to a novel method to multiplex long lifetime fluorescent dyes using time-resolved fluorescence (trf) detection. A combination of spectral and temporal differences in fluorescence emission is used to enhance the ability to separate signals in an assay from multiple dyes.
Molecular Devices, Llc
Novel fluorescent dyes, labeled conjugates and analytical methods
There are described novel fluorescent dyes, conjugates which include a radical of a dye and a biological or a synthetic moiety and diagnostic and in vivo assays utilizing such conjugates and other products including the dyes and conjugates.. .
Bioconjugates of heterocyclic compounds
The invention provides bioconjugates of heterocylic compounds such as s-adenosylmethionine and s-adenosylhomocysteine with biotin or digoxigenin. The bioconjugates also include carbon and nitrogen linker moieties of varying length that are used to attach such compounds to biotin or digoxigenin.
Monoazo dyes with cyclic amine as fluorescence quenchers
The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes.
Enzo Biochem, Inc.
Sample analyzing method and sample analyzer
A sample analyzing method includes: denaturing dna by heating a measurement specimen; bleaching the measurement specimen to inhibit autofluorescence from the measurement specimen; binding a fluorescent dye to a test substance in the measurement specimen; and capturing an image of fluorescence originated from the fluorescent dye by irradiating the measurement specimen with light. The dna denaturation treatment is performed before the bleaching..
Fluorescent dye additive for functional fluids
A fluorescent dye in combination with an aviation functional fluid. The fluorescent dye is present in the functional fluid from about 0.0001% by weight to about 1% by weight.
Synthesis of hyperbranched amphiphilic polyester and theranostic nanoparticles thereof
A method of making a hyperbranched amphiphilic polyester compound includes drying under vacuum a mixture of 2-(4-hydroxybutyl)-malonic acid and p-toluene sulphonic acid as catalyst. The vacuum is then released with a dry inert gas after drying.
University Of Central Florida Research Foundation, Inc.
Azatriangulenium salts as pet-quenched fluorescent probes
The present invention relates to a new class of substituted aza-triangulenium fluorescent dyes having a hydroxy group attached to an aryl as quenching group. The new substituted aza-triangulenium fluorescent dyes may be attached to a linker, conjugated to carrier molecule such as e.g.
A controlling hydrophobic contaminants by utilizing a fluorescent dye
The present invention pertains to a method of determining the quantity of hydrophobic contaminants in a papermaking process by utilizing a fluorescent dye, to a method of evaluating treatment chemicals for controlling hydrophobic contaminants by utilizing a fluorescent dye, and to a method of optimizing the amounts of treatment chemicals for reducing hydrophobic contaminants in a papermaking process by utilizing fluorescent dye.. .
Ecolab Usa Inc.
System and spray visualization
A spray visualization apparatus and method are disclosed. Unlike laser or doppler techniques requiring costly instrumentation, the herein disclosed technique is much simpler.
Multimeric protected fluorescent reagents
Multimeric protected fluorescent reagents and their methods of synthesis are provided. The reagents are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions.
Pacific Biosciences Of California, Inc.
Methods and systems for biological instrument calibration
In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites.
Life Technologies Corporation
System and methods for calibrating binding dyes
Some embodiments describe a computer-implemented method for calibrating a fluorescent dye. The method can comprise imaging a sample holder, loaded into an instrument, at more than one channel.
Life Technologies Corporation
Cell permeable, fluorescent dye
The dye (i) absorbs and emits light at wavelengths above 600 nm; (ii) possesses high photostability; (iii) has high extinction coefficients and high quantum yields; (iv) can be derivatized with different molecules; and (v) is membrane-permeable and shows minimal background binding to biomolecules and biomolecular structures.. .
Fluorescent probe for imaging lymph nodes
Disclosed is a near-infrared fluorescent imaging agent comprising an indocyanine-based fluorescent dye and a liposome. The near-infrared fluorescent imaging agent of the present invention demonstrates high fluorescence intensity and a long anchoring time in sentinel lymph nodes, thereby making it useful for detecting sentinel lymph nodes in sentinel lymph node navigation surgery.
Eisai R&d Management Co., Ltd.
Diagnosing and treating cancer
The invention relates to compositions and methods for diagnosing as well as treating cancer diseases associated with choline kinase (chok). Specifically, the invention relates to a composition comprising an intrinsically fluorescent choline kinase (chok) inhibitor or a chok inhibitor operably linked to a fluorescent dye.
The Trustees Of The University Of Pennsylvania
Nucleic-acid-sequence determination device and nucleic-acid-sequence determination method
A nucleic-acid-sequence determination device equipped with two light sources having different wavelengths, two detectors, and an optical system for irradiating a sample with light from the two light sources and guiding fluorescent light from a nucleic acid in the sample to the two detectors. The optical system is provided with a dichroic mirror for causing the fluorescent light from the nucleic acid in the sample to split, and guiding the split light to the two detectors.
Hitachi High-technologies Corporation
Optical selection of cells
Provided herein are photochemical crosslinkers and photocleavable crosslinkers and their uses in methods for cell selection from cell cultures. The photochemical crosslinkers comprise a fluorescent dye and a radical generator.
President And Fellows Of Harvard College
A quantum fluorescence background removal in dna melting analysis
A method of background removal from melting curves generated using a fluorescent dye is provided for analyzing a melting profile of a nucleic acid sample. The method comprises measuring the fluorescence of the nucleic acid sample as a function of temperature to produce a raw melting curve having a melting transition, the nucleic acid sample comprising a nucleic acid and a molecule that binds the nucleic acid to form a fluorescently detectable complex, the raw melting curve comprising a background fluorescence signal and a nucleic acid sample signal; and separating the background signal from the nucleic acid sample signal by use of a quantum algorithm to generate a corrected melting curve, the corrected melting curve comprising the nucleic acid sample signal..
University Of Utah Research Foundation
Compositions comprising fluorescent dyes associated with viral components for nerve imaging
Disclosed herein is a composition for imaging nerve cells. The composition includes a fluorescent dye; and a viral component including a neurotropic herpes varicellae unable to replicate in nerve cells, a viral protein of a neurotropic herpes varicellae unable to replicate in nerve cells, a capsid of a neurotropic herpes varicellae unable to replicate in nerve cells, or a combination thereof.
Novadaq Technologies Inc.
Near-infrared fluorescent surgical dye markers
The potential use of a pmma encapsulated near-infrared fluorescent dye includes endoscopic tattooing of intestinal neoplasms, location visualization of non-palpable breast lesions, and, but not limited to, location/visualization of soft tissue lesions in difficult anatomic regions. Commercially available fluorescent imaging systems, both open and laparoscopic, would be used in conjunction to fluoresce these marked tissues, augmenting the surgeon's ability to intraoperatively locate the correct lesion to resect..
Plastic scintillator with effective pulse shape discrimination for neutron and gamma detection
In one embodiment, a scintillator material includes a polymer matrix; and a primary dye in the polymer matrix, the primary dye being a fluorescent dye, the primary dye being present in an amount of 5 wt % or more; wherein the scintillator material exhibits an optical response signature for neutrons that is different than an optical response signature for gamma rays. In another embodiment, a scintillator material includes a polymer matrix comprising at least one of: polyvinyl xylene (pvx); polyvinyl diphenyl; and polyvinyl tetrahydronaphthalene; and a primary dye in the polymer matrix, the primary dye being a fluorescent dye, the primary dye being present in an amount greater than 10 wt %.
Fiducial-based correlative microscopy
A method is provided for preparing a sample for correlative optical and electron imaging and correcting aberrations in the imaging process due to sample deformation. Dye-coated fiducial markers are distributed throughout the sample volume.
Cyanated naphthalenebenzimidazole compounds
The present invention relates to cyanated naphthalenebenzimidazole compounds of the formula (i) and mixtures thereof, in which r1, r2, r3, r4, r5, r6, r7, r8, r9 and r10 are each independently hydrogen, cyano or aryl which is unsubstituted or has one or more identical or different substituents rar, where rar is as defined in the claims and in the description, with the proviso that the compounds of the formula i comprise at least one cyano group. The invention further relates to color converters comprising at least one polymer as a matrix material and at least one cyanated naphthalenebenzimidazole compound or mixtures thereof as a fluorescent dye, to the use of the color converters and to lighting devices comprising at least one led and at least one color converter..
Image processing apparatus and image processing method
An image processing apparatus includes: an interface unit configured to input an image signal from an imaging apparatus that exposes a specimen dyed with a fluorescent dye to excitation light and images fluorescence by a color imaging element; and a color correction circuit configured to retain information on a percentage of each of a component of a second color and a component of a third color with respect to a component of a first color corresponding to the excitation light in the image signal, which is determined in advance based on color filter spectral characteristics of the color imaging element, and reduce each of an amount corresponding to the percentage of the component of the second color and an amount corresponding to the percentage of the component of the third color from the input image signal.. .