|| List of recent Enzyme-related patents
| Enzymes that synthesize zingiberene|
The invention relates to nucleic acids encoding a zingiberene synthase that enables host cells and plants to make zingiberene that is useful in fragrances and for repelling or killing insects. The invention also relates to isolated zingiberene synthases and to methods for making zingiberenes..
| Modified plants with increased oil content|
Methods and means are provided to increase the oil content of plants, particularly oleaginous plants by preventing feedback inhibition by 18:1-coenzyme a or 18:1-acyl carrier protein of the acetyl coa-carboxylase enzyme in cells of these plants in various manners.. .
| Modular base-specific nucleic acid binding domains from burkholderia rhizoxinica proteins|
The present invention concerns new modular base-per-base specific nucleic acid binding domains (mbbbd) derived from newly identified proteins from the bacterial endosymbiont burkholderia rhizoxinica and their use for engineering nucleic acid processing enzymes, such as specific endonucleases or transcription activators.. .
| Production of oil in vegetative tissues|
The present invention relates to compositions and methods for providing rna interference (rnai) vectors comprising trigalactosyldiacylglycerol (tgd) biosynthesis enzyme constructs for increasing oil content of plants. Further, the use of tgd rnai silencing vectors in combination with co-expression of heterologous oil regulating transcription factors, such as wrinkled1, are contemplated to overcome the reduced growth and variable levels of embryonic lethality in plants with reduced tgd protein.
| Synthesis of new fucose-containing carbohydrate derivatives|
A method for the synthesis of a fucooligosaccharide glycosides by reacting a fucosyl donor with h-a-r1 or a salt thereof, wherein a and r1 are as defined herein, under the catalysis of an enzyme capable of transferring fucose is provided. The fucooligosaccharid glycoside compounds, or derivatives thereof, their use in the manufacture of human milk oligosaccharides, and a method of manufacture of human milk oligosaccharides, are also provided..
| P38map kinase inhibitors|
Or a pharmaceutically acceptable salt thereof, including all stereoisomers and tautomers, which is an inhibitor of p38 mitogen-activated protein kinase enzymes (referred to herein as p38 map kinase inhibitors), particularly the alpha and gamma kinase sub-types thereof, and its use in therapy, including in pharmaceutical combinations, especially in the treatment of inflammatory diseases, including inflammatory diseases of the lung, such as copd.. .
| Substituted quinazolines, the preparation thereof and the use thereof in pharmaceutical compositions|
The present invention relates to substituted quinazolines of formula (i) wherein x and y are defined as in claim 1, the tautomers, stereoisomers, mixtures and salts thereof, which have valuable pharmacological properties, particularly an inhibitory effect on the activity of the enzyme dipeptidylpeptidase-w (dpp-iv).. .
| Liquid detergent composition|
In a liquid detergent comprising a subtilisin and optionally a second (non-subtilisin) enzyme, the combination of a peptide aldehyde (or hydrosulfite adduct thereof) with a salt of a monovalent cation and a monovalent organic anion has a synergistic stabilizing effect on the subtilisin and/or the second enzyme. The improved enzyme stability is of particular interest in liquid detergent compositions where the enzyme would otherwise have poor storage stability..
| Thermophilic and thermoacidophilic metabolism genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods|
Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering metabolism in a cell using isolated and/or purified polypeptides and nucleic acid sequences from alicyclobacillus acidocaldarius..
| Methods and apparatus for building complex 3d scaffolds and biomimetic scaffolds built therefrom|
A novel method for building complex three-dimensional scaffolds for biomimetic applications such as in vitro organ growth, using a gelatin/sugar/water gel, ultraviolet radiation, and heat or enzyme is described. The method produces gelatin-sugar hydrogels demonstrating greater thermal stability, mechanical strength, and resistance to enzymatic degradation.
| Cancer tissue-derived cell mass and a process for preparing same|
Disclosed is a novel cell mass derived from a cancer tissue, which can reflect the in vivo behavior of a cancer cell correctly. Also disclosed is a process for preparing the cell mass.
| Enzymatic cleavage based lateral flow assays|
Methods and devices for detecting a target enzyme include an anchored or trapped peptide complex. The peptide complex includes an anchor particle immobilized on a sample analysis device or trapped in a reaction receptacle including a filter, a peptide, with at least one enzyme cleavage site for a target enzyme, bound to the anchor particle, at least one detectable label, and at least one first tag bound to the peptide on a side of the enzyme cleavage site opposite the anchor particle.
| Integrated processes for conversion of lignocellulosic biomass to bioproducts and systems and apparatus related thereto|
An integrated biological process for cellulosic bioproduct production is provided, which, in one embodiment, results in high ethanol productivity, enzyme recycling and reuse of yeast cells. The integrated process can be an integrated separate hydrolysis and fermentation (shf) process or an integrated fast simultaneous saccharification and co-fermentation (fsscf) process.
| Preparation of process of l-methionine|
The present invention relates to a method for producing l-methionine using a bio-synthesis process and a specific enzymatic process. More particularly, the present invention relates to a method for producing l-methionine with high yield by enzyme conversion reaction from l-methionine precursor in the presence of methyl mercaptan (ch3sh).
| C1-c2 organic acid treatment of lignocellulosic biomass to produce acylated cellulose pulp, hemicellulose, lignin and sugars and fermentation of the sugars|
A process for production of c5 and c6 sugar enriched syrups from lignocellulosic biomass and fermentation products therefrom is described. A lignocellulosic biomass is treated with a c1-c2 acid (e.g., acetic acid) with washing thereof with a c1-c2 acid miscible organic solvent, (e.g., ethyl acetate).
| Method for determining the spatiotemporal distribution of activity of a proteolytic enzyme in a heterogeneous system (variations), a device for realizing same and a method for diagnosing the defects in the hemostatic system on the basis of a change in the spatiotemporal distribution of activity of a proteolytic enzyme in a heterogeneous system|
The invention relates to the field of biotechnology. The method for determining the spatial and temporal distribution of the activity of a proteolytic enzyme in an in vitro heterogeneous system, such as blood or blood plasma, involves the introduction of a luminescent, fluorogenic or chromogenic substrate into a sample with the subsequent release of a detectable tag as the proteolytic enzyme cleaves the substrate, and the recording of the optical characteristics of the sample, which makes it possible to assess the spatial and temporal distribution of the activity of the enzyme.
| Method and apparatus for predicting susceptibility to a developmental disorder|
A nucleotide sequence signal amplification composition that includes an isolated, synthetic nucleotide sequence of greater than 7 nucleotides. The sequence is a fragment of seq id no:3 and further comprising a t nucleotide at position 1438 of seq id no:3 and one or more primers that bind to the synthetic nucleotide sequence, a thermostable dna polymerase, a restriction enzyme, or a combination thereof..
| Method and apparatus for multi-stage stabilization of whole grain flour|
The present invention relates a method and a system for a multi-stage treatment of whole grain flour to reduce enzyme activity, and more specifically, for the rapid treatment of whole grain flour to reduce the lipase enzyme activity for increasing shelf-life using microwave energy for at least one of the stages.. .
| Enzyme granules|
The present application relates to a steam treated pelletized feed composition comprising a granule comprising a core and a coating wherein the core comprises an active compound and the coating comprises a salt.. .
| Treating inflammation with a combination of an ellagitannin and hydrogen peroxide|
The teachings provided herein generally relate to site-activated binding systems that selectively increase the bioactivity of phenolic compounds at target sites. More particularly, the systems taught here include a phenolic compound bound to a reactive oxygen species, wherein the phenolic compound and the reactive oxygen species react at a target area in the presence of an oxidoreductase enzyme..
| Annexin a2 and tissue plasminogen activator for treating vascular disease|
The use of tpa to treat hemorrhagic transformation, neurotoxicity has been limited to short treatment time windows because a high dose of tpa required to generate sufficient amounts of the enzyme plasmin for clot lysis. The present invention combines tpa with recombinant annexin a2 resulting in thrombolysis without hemorrhagic transformation at delayed times after stroke.
| Coupling endonucleases with end-processing enzymes drives high efficiency gene disruption|
The present disclosure relates to the co-expression of an endonuclease with an end-processing enzyme for the purpose of enhanced processing of the polynucleotide ends generated by endonuclease cleavage.. .
| Compositions containing non-polar compounds|
Provided are compositions and methods for producing stable foods and beverages that contain high concentrations of additives such as essential fatty acids, including omega-3 fatty acids, omega-6 fatty acids, conjugated fatty acids, and other fatty acids; phytochemicals, including phytosterols and carotenoids; oil soluble vitamins; alpha lipoic acid; other oils; and coenzymes, including coenzyme q10, and other oil-based additives.. .
| Biosensor and measuring method employing same|
A biosensor is a bio sensor for measuring concentrations of first and second substrates in a sample, including a sensor unit including an insulating base plate, an electrode over the insulating base plate, and a reaction layer over the electrode; a timing unit for measuring time; and a calculating unit, in which the reaction layer contains a first enzyme for converting the first substrate into the second substrate, and a second enzyme acting on the second substrate or the converted substance obtainable by further converting the second substrate, and the calculating unit calculates concentrations of the first and the second substrates in the sample, on the basis of a first current value detected in the sensor unit at a first time and a second current value detected in the sensor unit at a second time.. .
|Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them|
The invention provides polypeptides having a lignocellulolytic activity, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a β-xylosidase), an arabinofuranosidase, and/or a glucose oxidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides that can enzymatically process (hydrolyze) sugarcane bagasse, i.e., for sugarcane bagasse degradation, or for biomass processing, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides.
|System and methods for pharmacogenomic classification|
The invention provides a system and methods for the determination of the pharmacogenomic phenotype of any individual or group of individuals, ideally classified to a discrete, specific and defined pharmacogenomic population(s) using machine learning and population structure. Specifically, the invention provides a system that integrates several subsystems, including (1) a system to classify an individual as to pharmacogenomic cohort status using properties of underlying structural elements of the human population based on differences in the variations of specific genes that encode proteins and enzymes involved in the absorption, distribution, metabolism and excretion (adme) of drugs and xenobiotics, (2) the use of a pre-trained learning machine for classification of a set of electronic health records (ehrs) as to pharmacogenomic phenotype in lieu of genotype data contained in the set of ehrs, (3) a system for prediction of pharmacological risk within an inpatient setting using the system of the invention, (4) a method of drug discovery and development using pattern-matching of previous drugs based on pharmacogenomic phenotype population clusters, and (5) a method to build an optimal pharmacogenomics knowledge base through derivatives of private databases contained in pharmaceutical companies, biotechnology companies and academic research centers without the risk of exposing raw data contained in such databases.
|Activation of sodium potassium atpase|
Activation sites on the alpha subunit of sodium potassium atpase have been discovered. It has also been discovered that certain antibodies that bind to the alpha subunit of sodium potassium atpase dramatically increase enzyme activity.
|Extended dicer substrate agents and methods for the specific inhibition of gene expression|
The invention provides compositions and methods for reducing expression of a target gene in a cell, involving contacting a cell with an isolated double stranded nucleic acid (dsna) in an amount effective to reduce expression of a target gene in a cell. The dsnas of the invention possess a pattern of deoxyribonucleotides (in most embodiments, the pattern comprises at least one deoxyribonucleotide-deoxyribonucleotide base pair) designed to direct the site of dicer enzyme cleavage within the dsna molecule.
|Selective metabolic approach to increasing oral bioavailability of phenylephrine and other phenolic bioactivities|
Presystemic metabolism in intestine of bioactives such as phenylephrine is avoided by administering a subject (human or animal) the bioactive (e.g., phenylephrine) in combination with one or more inhibitors of sulfation (e.g., sulfotransferase enzymes aka sults). This can also be enhanced be co-administering inhibitors of monoamine oxidases aka, maos, and uridine diphosphate glucoronysl transferases, aka ugts.
|Bicyclic pyridazine compounds as pim inhibitors|
The invention relates to bicyclic compounds of formulas i and i′, and salts thereof. In some embodiments, the invention relates to inhibitors or modulators of pim-1 and/or pim-2, and/or pim-3 protein kinase activity or enzyme function.
|Tricyclic inhibitors of poly(adp-ribose)polymerase|
The invention provides for compositions comprising phosphorous containing tricyclic compounds, including phthalazin-1(2h)-one derivatives. The compounds are potent inhibitors of the enzyme poly(adp-ribose)polymerase (parp), particularly parp-1 and potentially parp-2.
|Compositions and methods for treating cancer|
Provided herein are compounds used to inhibit the deamination enzyme responsible for the inactivation of therapeutic compounds, and methods of using them.. .
|Fish protein hydrolysate having a bone-stimulating and maintaining activity, nutraceutical and pharmacological compositions comprising such a hydrolysate and method for obtaining same|
The present invention relates to a fish protein hydrolysate having a biological activity of interest, in particular an effect on the stimulation and maintenance of bone. The fish protein hydrolysate is characterized in that it is obtained by enzymatic hydrolysis of at least one protein source selected from the fish species micromesistius poutassou, clupea harengus, scomber scombrus, sardina pilchardus, trisopterus esmarki, trachurus spp, gadus morhua, pollachius virens, melanogrammus aeglefinus and coryphaenoides rupestris, and the species of fish belonging to the order siluriformes, said enzymatic hydrolysis being carried out by means of an endopeptidase enzyme derived from bacillus subtilis.
|Viable gram negative bacteria with reduced proteolytic activity lacking outer membrane agonists of tlr4/md-2|
Viable gram-negative bacteria or components thereof comprising outer membranes that substantially lack a ligand, such as lipid a or 6-acyl lipidpolysaccharide, that acts as an agonist of tlr4/md-2. The bacteria may comprise reduced activity of arabinose-5-phosphate isomerases and one or more suppressor mutations, for example in a transporter thereby increasing the transporter's capacity to transport lipid iva or in membrane protein yhjd.
|Kits and methods for generating 5' capped rna|
The present invention relates to kits and methods for efficiently generating 5′ capped rna having a modified cap nucleotide and for use of such modified-nucleotide-capped rna molecules. In particular, the present invention provides kits and methods for capping rna using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme.
|Sequence based genotyping based on oligonucleotide ligation assays|
The invention relates to a method for the detection of a target nucleotide sequence in a sample based on an oligonucleotide ligation assay wherein probes are used that contain (a combination of) sequence-based identifiers that can identify the sample and the target sequence (i.e. Locus and/or allele combination) wherein after the ligation step, the ligated probes, or after amplification, the amplified ligated probes, are restricted using restriction enzymes to cut of part of the probes and continue with those parts (identifiers and target sequence) that contain the relevant information in the sequencing step..
|Filamentous fungi having an altered viscosity phenotype|
Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications..
|Agents and method for treating inflammation-related conditions and diseases|
Gene-modified, inflammation-specific monocytes that comprise a 1-alpha-hydroxylase gene, where the 1-alpha-hydroxylase gene is expressed to produce functional 1-alpha-hydroxylase enzyme when the monocytes transdifferentiate into gene-modified, inflammation-specific macrophages. Gene-modified, inflammation-specific macrophages that comprise a 1-alpha-hydroxylase gene.
|Dynamic mixing and electroporation chamber and system|
The present invention relates to an apparatus for mixing cells and exogenous material for delivery of the exogenous material into cells using electroporation in a manner that preserves cell viability and integrity of the exogenous material. The exogenous material can be polynucleotides, peptides, proteins or other pharmaceutical molecules.
|Dried spore germinative compound mixtures|
In one aspect, the present invention is directed to a dried intimate mixture comprising a bacteria spore and a germinative compound, and methods for preparing the intimate mixture. In another aspect, this invention is directed to a composition comprising such an intimate mixture.
|Recombinant butyrylcholinesterases and truncates thereof|
Isolated nucleic acids encoding polypeptides that exhibit butyrylcholinesterase (bche) enzyme activity are disclosed, along with molecular criteria for preparing such nucleic acids, including codon optimization. Methods of preparing modified and/or truncated bche molecules having selected properties, especially selective formation of monomers, are also described.
|Glucose dehydrogenase and glucose sensor with same|
A glucose dehydrogenase, which is an enzyme that has high substrate specificity, can be produced at a low cost, is not affected by oxygen dissolved in a measurement sample and, in particular, has superior thermal stability is obtained by culturing a microorganism belonging to the genus burkholderia. A glucose sensor utilizing the glucose dehydrogenase protein is described..
|Transcription factors for cellulosic enzyme production|
Provided herein are methods and compositions for increasing the production of one or more cellulases from a fungal host cell. The disclosure is based, on the surprising discovery that mis-expression of the transcriptional regulator clr-2 in a filamentous fungal cell was able to induce expression of cellulase genes under non-inducing or starvation conditions, resulting in increased secretion of cellulases from the cell.
|Ubiquitin chain assembly|
There is provided a method for producing free polyubiquitin chains linked through a single desired lysine residue, comprising the steps of: (a) selecting an e3 ubiquitin ligase enzyme which is homologous to mammalian hect e3 ligases and possesses the desired lysine residue specificity; (b) incubating the e3 enzyme with an e1 ubiquitin activating enzyme, an e2 ubiquitin conjugating enzyme and monomeric ubiquitin; and (c) if undesired linkages are present, removing the undesired linkages by exposure to a dub enzyme having the appropriate specificity.. .
|Method and culture device for detecting yeasts and molds|
A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents.
|Reagentless ceria-based colorimetric sensor|
A colorimetric reagent in the form of nanoparticles, composite nanoparticles, and nanoparticle coatings, including methods of use, methods of preparation, deposition, and assembly of related devices and specific applications. The colorimetric reagent comprises cerium oxide nanoparticles which are used in solution or immobilized on a solid support, either alone or in conjunction with oxidase enzymes, to form an active colorimetric component that reacts with an analyte to form a colored complex.
|Diagnosis method of active tuberculosis|
The present invention relates to a method for the in vitro diagnosis of active tuberculosis, comprising a step of contacting lymphocytes of a patient suspected to have active tuberculosis with at least one protein of mycobacteria, said protein being an enzyme having a lipolytic activity, and a step of detecting the presence of specific activated lymphocytes.. .
|Competition-based detection assays|
Disclosed herein are methods and kits which are useful for detecting presence of an enzyme and the relative amount of glycan associated with the enzyme in a test sample based upon the enzyme's ability to competitively inhibit the binding of a ligand in such test sample. The present invention provides the ability to evaluate cell culture conditions and optimize the desired glycoform content of recombinantly prepared enzymes..
|Ubiquitin chain analysis|
There is described a method for analyzing ubiquitin polymers using linkage-specific deubiquitinase enzymes. Novel specificities of deubiquitinase enzymes are also provided..
|Methods of using improved polymerases|
This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase..
|Enzymatic conversion of blood group a, b, and ab red blood cells using alpha-n-acetylgalactosaminidases and alpha-galactosidases with unique substrate specificities and kinetic properties|
This invention relates to enzymatic removal of type a and b antigens from blood group a, b, and ab reactive cells in blood products, and thereby converting these to non-a and non-b reactive cells. The invention further relates to using unique α-n-acetylgalactosaminidases and α-galactosidases with superior kinetic properties for removing the immunodominant monosaccharides of the blood group a and b antigens and improved performance in enzymatic conversion of red blood cells.
|Method of hydration; infusion packet system(s), support member(s), delivery system(s), and method(s); with business model(s) and method(s)|
Liquid activated infusion packet(s)/system: promoting hydration, containing active and/or inactive ingredients and/or a support member(s). Infusion packet(s)/system is one or more individual compartments, and/or group(s), whereby the enveloping material(s) may be totally or partially dissolvable, edible, transparent, opaque, decorated, etc.
|Cheese and preparing the same|
The present invention relates to a process for producing cheese, comprising the steps of: providing a first raw material liquid; providing a second raw material liquid; treating the first raw material liquid with a protein crosslinking enzyme to provide an enzyme-treated raw material liquid; mixing the enzyme-treated raw material liquid with the second raw material liquid to provide cheese milk; processing the cheese milk into cheese. The process produces cheese in improved yields while retaining the organoleptic properties of cheese unchanged.
|Methods for treating niemann-pick type c disease|
The present invention includes methods for treating niemann-pick type c disease through administration of inhibitors of acyl-coenzyme a:cholesterol acyltransferase 1 (acat1). Acat inhibitors are used to treat symptoms of niemann-pick type c disease and prolong survival of patients with the disease, either alone or in combination with other treatments..
|Methods for localized modification of tissue products|
Methods for treating tissue matrices and tissue matrices produced according to the methods are provided. The methods can include treating select portions of a tissue matrix with a cross-linking agent and/or a proteolytic enzyme to produce a tissue matrix with variable mechanical and/or biological properties..