|| List of recent Enzyme-related patents
| Sorghum plants having a mutant polynucleotide encoding the large subunit of mutated acetohydroxyacid synthase protein and increased resistance to herbicides|
A sorghum seed comprising in its genome at least one polynucleotide encoding a polypeptide having an alanine to threonine substitution at position 93 of the sorghum ahas protein large subunit. The plant has increased resistance to one or more herbicides, for example from the imidazolinone group, as compared to wild-type sorghum plants.
| Compositions and methods for the biosynthesis of vanillan or vanillin beta-d-glucoside|
Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express a mutant arom polypeptide and/or mutant catechol-o-methyltransferase polypeptide alone or in combination with one or more vanillin biosynthetic enzymes or udp-glycosyltransferases (ugts). Such microorganisms, plants, or plant cells can produce vanillin or vanillin beta-d-glucoside..
| Hemostatic and biodegradable bandages and bandage implants|
In one embodiment, the present invention is a multi-layered bandage including a wound-contacting first layer which is porous, fluid-permeable, biodegradable, and capable of accelerating hemostasis and wound healing, and a second layer formed from an absorbent material which is releasably secured to the first layer. The first layer includes a porous, fluid-permeable, biodegradable lattice structure formed from a homogeneous mixture of collagen and a clotting enzyme which, as healing occurs, may be replaced by new tissue.
| Enzyme-degradable polymer and application thereof|
The present invention belongs to the biomedicine field and specifically concerns an enzyme-degradable polymer and the application thereof. To solve the problem of low sensitivity of the existing assay reagents, the present invention provides an enzyme-degradable polymer and the related application of the polymer.
| 5- or 6-substituted 3-hydroxy-2(1h)-pyridinones as d-amino acid oxidase (daao) inhibitors in therapy of diseases such as schizophrenia, cognitive disorder and paid|
The present invention provides compounds of formula (i) and pharmaceutically acceptable salts thereof, wherein r1 is hydrogen or fluorine, one of a and b is r2, the other is a group —x—y—r3. R3 represents a carbocyclic or heterocyclic ring.
| Cytochrome p450 oxidase inhibitors and uses thereof|
Or pharmaceutically acceptable salts, solvates or prodrugs thereof, and methods of using the same to inhibit the metabolizing activities of cyp enzymes. The present invention also features methods of using these compounds, salts, solvates or prodrugs to improve the pharmacokinetics of drugs that are metabolized by cyp enzymes..
| Aminopyrimidines as syk inhibitors|
The present invention provides novel pyrimidine amines of formula (i) which are potent inhibitors of spleen tyrosine kinase, and are useful in the treatment and prevention of diseases mediated by said enzyme, such as asthma, copd and rheumatoid arthritis.. .
| Bile acid recycling inhibitors for treatment of pediatric cholestatic liver diseases|
Provided herein are pediatric dosage forms for use in the treatment of a pediatric cholestatic liver disease by non-systemically administering to an individual in need thereof a therapeutically effective amount of the pediatric dosage form comprising an apical sodium-dependent bile acid transporter inhibitor (asbti) or a pharmaceutically acceptable salt thereof. Also provided are said pediatric dosage form for use in the treatment of a pediatric liver disease, for use in decreasing the levels of serum bile acids or hepatic bile acids, for use in the treatment of pruritis, for use in reducing liver enzymes or bilirubin comprising non-systemically administering to an individual in need thereof a therapeutically effective amount of a pediatric formulation comprising an asbti or a pharmaceutically acceptable salt thereof..
| Use of nrf2 inducers to treat epidermolysis bullosa simplex and related diseases|
The present invention relates to methods and compositions for the prevention and treatment of keratin-based skin diseases. In particular, the application describes compositions and methods of treating a patient suffering from skin blistering comprising the use of phase ii enzyme inducers..
| Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation|
The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are compositions and methods related to components of a crispr complex particularly comprising a cas ortholog enzyme..
| Method for production of isoprenoid compounds|
The present invention is directed to variant squalene synthase enzymes, including saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the s.
| Bioconversion process for producing nylon-7, nylon-7, 7 and polyesters|
Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional c7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional c7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters..
| Enzymatically catalyzed method of preparing mono-acylated polyols|
The present invention relates to a biocatalytic method of preparing a mono-acylated polyol catalyzed by triacylglycerol lipase mutants, as for example derived from candida antarctica lipase b (calb); a biocatalytic method of enantioselectively preparing an asymmetric mono-acylated polyol, catalyzed by the same enzyme mutants; as well as the use of a mutated triacylglycerol lipase in a method of preparing mono-acylated polyols. The invention also provides novel mutants, coding sequences thereof, and recombinant microorganisms carrying said coding sequences..
| Recominant ralstonia eutropha capable of producing polyactic and acid or polylatic acid polymer, and method for producing polyactic acid or polylatic acid copolymer using the same|
Provided are a recombinant ralstonia eutropha capable of producing polylactate or a hydroxyalkanoate-lactate copolymer, and a method of preparing polylactate or a hydroxyalkanoate-lactate copolymer using the same. The recombinant ralstonia eutropha, which is prepared by introducing a gene of an enzyme converting lactate into lactyl-coa and a gene of a polyhydroxyalkanoate (pha) synthase using lactyl-coa as a substrate thereto, may be cultured, thereby efficiently preparing a lactate polymer and a lactate copolymer..
| Hybrid organic-inorganic system for producing biofuels|
The present invention provides for a system for converting co2 and h2 to one or more biologically derived compounds. In some embodiments, the system comprises a host cell comprising one or more nucleic acids encoding genes for a recombinant surface display protein which is capable of tethering an electrocatalyst molecule, such as a cobalt(ii) complex supported by tetradentate polypyridyl ligand 2-bis(2-pyridyl)(methoxy)methyl-6-pyridylpyridine (py4), and enzymes for synthesizing a biologically derived compound, such as an alkane, alcohol, fatty acid, ester, or isoprenoid..
| In vivo and in vitro olefin cyclopropanation catalyzed by heme enzymes|
The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product.
| Oxidation and amination of primary alcohols|
The present invention relates to a method comprising the steps a) providing a primary alcohol of the formula ho—(ch2)x—r1, wherein r1 is selected from the group consisting of —oh, —sh, —nh2 and —coor2, x is at least 3 and r2 is selected from the group consisting of h, alkyl and aryl, b) oxidizing the primary alcohol by contacting it with an nad(p)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, wherein the nad(p)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a primary alcohol.. .
| The use of phosphoketolase for producing useful metabolites|
The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme a, such as l-glutamic acid, l-glutamine, l-proline, l-arginine, l-leucine, l-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of d-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium..
| Dual cofactor specific ribitol dehydrogenase and use thereof|
There are provided a novel ribitol dehydrogenase, a residue determining double coenzyme specificity, and a method for preparing l-ribulose using the same, and more particularly, to a ribitol dehydrogenase producing rare sugars, nucleic acid molecules encoding the same, a vector including the nucleic acid molecules, a transformant including the vector, a mutant of the ribitol dehydrogenase, and a method for preparing l-ribulose using the ribitol dehydrogenase. The ribitol dehydrogenase having double coenzyme specificity, which is derived from zymomonas mobilis, can effectively be used for preparing high-priced rare sugars and an investigation of coenzyme specificity determinants for the ribitol dehydrogenase is applied for all of dedydrogenases as a based technique..
| Analytical method for fab and fab' molecules|
A method of measuring acidic species generated by degradation of a fab or fab′ component of a fab-peg or a fab′-peg is provided. The method involves: a) cleaving peg and a linker from the fab-peg or fab′-peg with an enzyme; b) optionally separating the peg and linker from the fab or fab′ to provide isolated fab or fab′; and c) quantitatively analyzing acidic species associated with the cleaved fab or fab′ and/or the cleaved peg..
| System and method for integration of mobile device imaging with microchip elisa|
A system and method for analyzing a biomarker in a biological sample is provided. A biological sample is loaded onto a microchip and an enzyme-linked immunosorbent assay specific to the biomarker is performed on the microchip.
| Antibody for detecting epithelial ovarian cancer marker and method for diagnosing epithelial ovarian cancer|
It is intended to find a highly specific epithelial ovarian cancer marker and to provide an antibody capable of specifically recognizing and detecting the marker or a fragment of the antibody. The present invention provides an anti-β1,6-n-acetylglucosaminyltransferase 5b antibody for diagnosis of epithelial ovarian cancer, i.e., an antibody for detection of a glycosyltransferase β1,6-n-acetylglucosaminyltransferase 5b as an epithelial ovarian cancer marker.
| Reversible terminator molecules and methods of their use|
The present invention relates generally to methods of sequencing of polynucleotides and compounds, compositions and kits useful for sequencing of polynucleotides. The chemical compounds include nucleotide and nucleoside analogs which possess a blocking group covalently attached to the 3′ hydroxyl of the sugar moiety.
| Kits and methods for in vitro analyte detection using precipitating compound and polarizers|
The invention provides inexpensive kits and methods for determination of an analyte in a sample with very high sensitivity. Analyte can be determined using an analyte binding member such as an antibody or an oligonucleotide, which can be directly or indirectly linked to an enzyme.
| Polypeptide having phytase activity and increased temperature resistance of the enzyme activity, and nucleotide sequence coding said polypeptide|
The invention relates to a recombinant dna molecule encoding a polypeptide having phytase activity and increased temperature stability and increased proteolytic stability of the enzyme activity. The dna sequence has been obtained by variation of the mature wild-type e.
| Compositions of engineered human arginases and methods for treating cancer|
Compositions and methods for the treatment of cancer are described, and, more preferably, to the treatment of cancers that do not express, or are otherwise deficient in, argininosuccinate synthetase, with enzymes that deplete l-arginine in serum. In one embodiment, the present invention contemplates an arginase protein, such as a human arginase i protein, comprising at least one amino acid substitution and a metal cofactor, said protein comprising an increased catalytic activity when compared with a native human arginase i..
| Preparation of stabilized catalase enzymes using polyvinyl alcohol|
There is provided a method of producing a stabilized catalase enzyme. In the method, a substrate is thoroughly mixed with phosphate borate and catalase, rinsed with water and the solids dried.
| Compositions containing non-polar compounds|
Provided herein are compositions and methods for preparing foods and beverages that contain additives, such as nutraceuticals, pharmaceuticals, and supplements, such as essential fatty acids, including omega-3 fatty acids, omega-6 fatty acids, conjugated fatty acids, and other fatty acids; phytochemicals, including phytosterols; other oils; and coenzymes, including coenzyme q10, and other oil-based additives.. .
| Inhibition and enhancement of reprogramming by chromatin modifying enzymes|
Methods and compositions are provided for the production of stem cells and induced pluripotent stem cells, and for uses thereof.. .
| Methods for making construction materials using enzyme producing bacteria|
Methods for producing construction material utilizing loose pieces of aggregate (30), enzyme producing bacteria, an amount of urea and an amount of calcium ions. A first solution is prepared which includes urease which is formed by enzyme producing bacteria.
| Modified arsenite oxidase and a biosensor for detecting arsenite|
The present invention provides an arsenite oxidase enzyme modified to prevent translocation by modification of a translocation signal sequence. A microorganism modified to express the heterologous arsenite oxidase enzyme is also provided by the invention, together with a device for detecting the presence of arsenite in a sample..
|Methods and compositions for targeted polynucleotide modification|
A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism..
|Novel antithrombotic and antiatherosclerotic agent and method for producing same (variants)|
The use of 2-[2-cyano-2-[5-(hydroxymethyl)-3-methyl-1,3-oxazolidin-2-ylidene]ethylidene]indolin-3-one of formula i is proposed as a biologically active compound exhibiting the properties of exogenous nitric oxide donor, a platelet aggregation inhibitor, also exhibiting antihypertensive activity and activating activity towards the soluble guanylate cyclase enzyme. Variants of methods for synthesizing the above compound are also proposed..
|Process for producing novel sialo-sugar chain|
The present invention provides a novel sialo-sugar chain, a process for producing the sialo-sugar chain, and a device for producing the sialo-sugar chain. A sialo-sugar chain can be easily and efficiently mass-produced by reacting a sugar wherein a hydroxy groups is substituted with an alkynyl group (herein sometimes referred to as “alkynylated sugar”) with a specific sialic acid donor in the presence of a sialic acid-introducing enzyme..
|Chemoenzymatic synthesis of heparin and heparan sulfate analogs|
The present invention provides a one-pot multi-enzyme method for preparing udp-sugars from simple sugar starting materials. The invention also provides a one-pot multi-enzyme method for preparing oligosaccharides from simple sugar starting materials..
|N-alkylthio beta-lactams, alkyl-coenzyme a asymmetric disulfides, and aryl-alkyl disulfides as anti-bacterial agents|
The present invention provides n-alkylthio β-lactams and disulfide compounds (e.g., alkyl-coenzyme a asymmetric disulfides or aryl-alkyl disulfides), compositions containing such compounds, and methods of their use as anti-bacterial agents.. .
|Compositions and methods of treating gliomas|
The present invention provides, inter alia, methods for treating or ameliorating the effects of a glioma. Methods of this invention include administering to a subject in need thereof an effective amount of a first active agent, such as e.g., an angiotensin receptor blocker, an antifungal agent, a bisphosphonate, an oxytocin inhibitor, an interleukin-1 (il-1) inhibitor, a cyclooxygenase inhibitor, an α2δ voltage-dependent calcium channel (vdcc) inhibitor, a dihydroorotate dehydrogenase inhibitor, a calcium channel blocker, a renal sodium-chloride symporter inhibitor, an a2 adrenergic agonist, a phenothiazine antipsychotic, a calcineurin inhibitor, a 5-ht agonist, an angiotensin-converting enzyme (ace) inhibitor, a direct rennin inhibitor, or combinations thereof, and a second active agent, which is a chemotherapeutic agent.
|Antineoplastic hydrogels, and enzyme-instructed preparations thereof|
Disclosed is a general methodology to create nanofibers of therapeutic molecules that have a dual role, as both the delivery vehicle and the drug itself. It is shown that with proper molecular design, the integration of enzymatic reaction and self-assembly provides a powerful method to create molecular hydrogels of clinically-used therapeutics without compromising their bioactivities.
|Method for screening and quantifying isoprene biosynthesis enzyme activity|
Therefore, the invention can be advantageously used in the protein engineering technology for enzyme modification. Particularly, it can provide a quantitative investigation of enzymatic activity, and thus can be applied to molecular evolution technology..
|Methods for preparing single domain antibody microarrays|
The present invention relates to a method for preparing sd ab microarray comprising the step consisting of: —i) providing a host cell capable of expressing a biotinylation enzyme —ii) transforming said host cell with a nucleic acid encoding for a fusion protein wherein a single domain antibody is fused at its carboxy terminal end to a biotinylation peptide —iii) culturing said host cell in presence of biotin in such a way that said fusion protein and biotinylation enzyme are expressed, resulting in biotinylation of said fusion protein —iv) lysing said host cell as cultured at step iii) —v) spotting the lysate obtained at step iv) on a solid sup port coated with an agent selected from the group consisting of avidin, streptavidin and/or any art known derivative of these agents a further object of the invention relates to a sd ab microarray obtainable by the method of the invention.. .
|Benzamide compounds and related methods of use|
Benzamide compounds and derivatives thereof, as can be used for selective inhibition of the sirt2 enzyme and/or therapeutic use in the treatment of huntington's disease.. .
|Production method for organic acid using coa-transferase|
The present invention relates to a method of producing organic acids, comprising biochemical synthesis of organic acid-coa using acetyl-coa as a substrate and conducting coa elimination reaction of the organic acid-coa using coa transferase in the presence of acetic acid, in which organic acid is obtained by culturing transformed microorganisms which have an enzyme gene cluster for the synthesis of organic acid-coa using acetyl-coa as a substrate and a coa transferase gene.. .
|System and method for the production of alkyl esters|
A method of producing alkyl esters including processing a high free fatty acid feedstock including a mixture of triglycerides and free fatty acids to remove water and solids to create a processed feedstock. The processed feedstock is introduced and mixed into a reaction vessel, the reaction vessel includes water, at least one enzyme, and alcohol.
|Cellulolytic enzyme compositions and uses thereof|
The present invention relates enzyme composition comprising a cellulolytic preparation and an acetylxylan esterase (axe); and the used of cellulolytic enzyme compositions for hydrolyzing acetylated cellulosic material. Finally the invention also relates to processes of producing fermentation products from acetylated cellulosic materials using a cellulolytic enzyme composition of the invention..
|Aspergillus fumigatus cellulolytic enzyme compositions and uses thereof|
The present invention relates to recombinant trichoderma host cells producing aspergillus fumigatus cellulolytic enzyme compositions and methods of producing and using the compositions.. .
|Process for treating a substrate with an enzyme|
In a process for hydrolyzing plant material in aqueous solution or suspension with an enzyme, the enzyme is delivered in solid form (e.g., as a spray-dried powder) in closed containers (such as paper bags or cardboard boxes), which are added directly in the process (i.e., addition of whole boxes/bags). The invention is particularly amenable to the production of first or second-generation bioethanol..
|Method for generating human milk oligosaccharides (hmos) or precursors thereof|
A method for generating human milk oligosaccharides (hmos) or precursors thereof, compounds obtainable by the method, and uses and compositions involving such compounds. The method comprising the steps of a) providing at least one donor selected from the group of compounds of any of formulae 5 to 10, b) providing at least one acceptor from a group of lactose, lnt, lnnt and derivatives thereof, c) providing at least one enzyme comprising a transglycosidase activity and/or a glycosynthase activity, d) preparing a mixture of the at least one donor, at least one acceptor and at least one enzyme provided in steps a), b) and c); and e) incubating the mixture prepared according to step d)..
|Systems and methods for hydrolysis of biomass|
Systems and methods are disclosed for treating lignocellulosic biomass to be supplied to a fermentation system for production of a fermentation product. The systems and methods comprise pre-treating the biomass into pre-treated biomass and separating the pre-treated biomass into a liquid component comprising sugars and a solids component comprising cellulose and lignin.
|Methods for nucleic acid manipulation|
A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor.
|Methods for producing modified glycoproteins|
Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts.
|Method of extraction of an enzyme from plant or animal tissue|
This invention provides a method for the extraction and detection of a peptide from transgenic plant tissues wherein a non-immunogenic solubility-promoting compound is used to release the enzyme into the solution fraction during the purification process. In some embodiments, this invention provides a method for the extraction and detection of the enzyme amy797e, which is a heterologous thermo-tolerant α-amylase, from the tissues of corn event 3272 using a non-immunogenic amylase during the purification process.
|High throughput genome-wide translocation sequencing|
Provided are methods for high-throughput screening to determine locations of double-stranded dna breaks (dsbs) and translocations in genomes caused by different agents, such as enzymes.. .
|Recombinase polymerase amplification|
This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes.
|Coenzyme-linked glucose dehydrogenase and polynucleotide encoding the same|
The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to fad to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor..
|Product and process for its manufacture|
The invention relates to a process for the preparation of a soured milk product by means of physical modification of milk raw material and a crosslinking enzyme that strengthens the texture. The invention also relates to a milk product that contains physically modified milk raw material fat globules and has been treated with a crosslinking enzyme..
|Methods and products for in vivo enzyme profiling|
The present invention relates to methods and products associated with in vivo enzyme profiling. In particular, the invention relates to methods of in vivo processing of exogenous molecules followed by detection of signature molecules as representative of the presence of active enzymes associated with diseases or conditions.
|Methods of promoting immune tolerance|
Compositions including a polynucleotide combined with a vehicle and methods of their use to induce a suppressive immune response are provided. In some embodiments the compositions induce an increase in expression of indoleamine 2,3 dioxygenase (ido) enzyme activity in cells.
|Evaluation and improvement of nuclease cleavage specificity|
Engineered nucleases (e.g., zinc finger nucleases (zfns), transcriptional activator-like effector nucleases (talens), and others) are promising tools for genome manipulation and determining off-target cleavage sites of these enzymes is of great interest. We developed an in vitro selection method that interrogates 1011 dna sequences for their ability to be cleaved by active, dimeric nulceases, e.g., zfns and talens.
|Rna interferases and methods of use thereof|
The present invention is directed to the discovery of a novel family of enzymes designated herein as mrna interferases that exhibit endoribonuclease activity. The novel finding of the present inventors, therefore, presents new applications for which mrna interferase nucleic and amino acid sequences, and compositions thereof may be used to advantage.