|| List of recent Enzyme-related patents
| Lipid nanoparticle compositions and methods for mrna delivery|
Disclosed herein are compositions and methods for modulating the production of a protein in a target cell. The compositions and methods disclosed herein are capable of ameliorating diseases associated with protein or enzyme deficiencies..
| Five-membered heterocycles useful as serine protease inhibitors|
Or a stereoisomer or pharmaceutically acceptable salt or solvate form thereof, wherein the variables a, l, z, r3, r4, r6, r11, x1, x2, and x3 are as defined herein. The compounds of formula (i) are useful as selective inhibitors of serine protease enzymes of the coagulation cascade and/or contact activation system; for example thrombin, factor xa, factor xia, factor ixa, factor viia and/or plasma kallikrein.
| Inhibitors of mtor kinase as anti -viral agent|
The present invention provides methods for treating or preventing viral infections using modulators of host cell enzymes relating to mtor. The invention also provides methods for treating or preventing viral infections using modulators of host cell enzymes relating to mtor and modulators of the unfolded protein response..
| Dual action inhibitors against histone deacetylases and 3-hydroxy-3-methylglutaryl coenzyme a reductase|
Disclosed herein are novel compounds of formula (i), and uses thereof. The compounds of formula (i) are inhibitors of histone deacetylases (hdacs) and 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) reductase (hmgr).
| Opioid prodrugs with heterocyclic linkers|
The embodiments provide prodrug compounds of formulae i-xv. The present disclosure also provides compositions, and their methods of use, where the compositions comprise a prodrug compound of formulae i-xv that provides controlled release of an opioid.
| Particulate composition|
Enzymes tend to be inactivated during wash by a bleach catalyst in combination with a source of organic peroxyacids. The risk of enzyme inactivation by active bleach catalyst is reduced when the release of the enzyme into the wash solution is delayed.
| Novel yeast strains|
There is provided an alkene-producing yeast cell comprising a bacterial fatty acid decarboxylase enzyme, which may comprise the amino acid sequence seq id no:1 or a functional variant or portion thereof. The alkene may have 15, 17 or 19 carbon atoms.
| Method for viscosity reduction in co-fermentation ethanol processes|
The present disclosure provides methods and compositions for reducing the viscosity of biomass process streams in an ethanol production process. The method comprises adding cellulase enzymes to a biomass feedstock that is fermented to produce ethanol, generating whole stillage and thin stillage streams from the post-fermentation biomass, and adding an additional enzyme or enzyme cocktail that reduces the viscosity of the whole stillage stream, thin stillage stream, concentrated thin stillage stream, and/or the syrup stream generated by evaporating the thin stillage..
| Genes encoding key catalyzing mechanisms for ethanol production from syngas fermentation|
Gene sequences of key acetogenic clostridial species were sequenced and isolated. Genes of interest were identified, and functionality was established.
| Method for producing polyhydroxyalkanoic acid|
Embodiments of the invention relate to the microbial production of polyhydroxyalkanoic acids, or polyhydroxyalkanoates (pha), from substrates which cannot be used as a source of carbon and/or energy for microbial growth or pha synthesis and which have microbial and environmental toxicity. According to one embodiment of the invention, a process for the production of pha is provided wherein an enzyme such as methane monooxygenase is used to convert volatile organic compounds into pha through the use of microorganisms that are unable to use volatile organic compounds as a source of carbon for growth or pha production..
| Compositions and methods for producing chemicals and derivatives thereof|
The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into d-glucarate (step 7); an enzymatic conversion of 5-ketogluconate (5-kga) into l-iduronic acid (step 15); an enzymatic conversion of l-iduronic acid into idaric acid step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-ddh) (step 16).
| Stable nad/nadh derivatives|
The present invention provides for stable nicotinamide adenine dinucleotide (nad/nadh) and nicotinamide adenine dinucleotide phosphate (nadp/nadph) derivatives of formula (i), enzyme complexes of these derivatives and their use in biochemical detection methods and reagent kits.. .
| Methods and kits for detecting mastitis|
Methods and kits for determining if one or more animals have mastitis and for monitoring animals and the quality of the milk they produce are disclosed. Kits and test assays disclosed are used to determine the quantity of proteasomes and proteins thereof, the activity of proteasome enzymes, the quantity of proteasome bound and regulating proteins, and the quantity of ubiquinated protein.
| Molecular conjugate|
A method is disclosed for making a conjugate of two molecules using a hydrazide thiol linker. In a particular working embodiment, an fc-specific antibody-enzyme conjugate is made using the method and demonstrated to provide exceptional staining sensitivity and specificity in immunohistochemical and in situ hybridization assays..
| Anti-tnf-alpha therapy for the mucopolysaccharidoses and other lysosomal disorders|
The present invention relates to methods of treating a subject with a lysosomal disorder, by administering an agent for enzyme replacement therapy and an agent for anti-tnf-α therapy; by administering a pentosan polysulfate therapy; or by administering a substrate reduction therapy and an anti-tnf-α therapy. The invention further relates to a method of reducing inflammatory cytokines in a subject with a lysosomal disorder that is being treated by enzyme replacement therapy, by administering an agent for anti-tnf-α therapy..
| Use of plp with peg-rmetase in vivo for enhanced efficacy|
This invention relates to methods of modifying pyridoxal 5′ phosphate (plp) dependent enzymes to extend the serum half-life of the enzyme, extend the in vivo period of methionine depletion in a host, and decrease the immunogenicity of the enzyme. A preferred plp-dependent enzyme to be modified is a methioninase, preferably a recombinant methioninase (rmetase).
| Formulations containing saccharomyces boulardii and superoxide dismutase (sod) to control obesity|
Disclosed is a composition containing saccharomyces cerevisiae var boulardii and the enzyme superoxide dismutase.. .
| Method for diagnosing or treating tumors using sphingomyelin containing liposomes|
The invention provides compositions and methods for diagnosing tumors and augmentic therapeutic intervention and measuring response to cell stress using sphingomyelin containing liposomes. The liposomes can include radiotracers, contrast agents, chromophores, dyes, enzyme substrates, therapeutic agents, chemotherapeutic agents or dna segments.
|Enzymatic production or chemical synthesis and uses for 5,7-dienes and uvb conversion products thereof|
Provided herein are steroidal compounds that are androsta-5,7-dienes or a pregna-5,7-dienes and ultraviolet b (uvb) conversion products thereof which includes pharmaceutical compositions of the steroidal compounds as shown in tables 1 and 2. Also provided is a method for producing hydroxylated metabolites of cholecalciferol or ergocalciferol via the p450scc (cyp11a1) or cyp27b1 enzyme systems where the hydroxylase has an activity to hydroxylate position c20 of a secosteroid or its 5,7-dieneal precursor and the hydroxylated metabolites so produced.
|Reduction of culture viscosity by manganese addition|
A method of producing an enzyme of interest in a fed-batch cultivation comprising: a) cultivating a microorganism in a culture medium conducive to its growth wherein the microorganism produces the enzyme of interest; and b) adding a manganese compound to the culture medium one or more times during the cultivation.. .
|Method for preparing volatile fatty acids from the pre-treated extracts of marine biomass residue|
A method of preparing a volatile fatty acids (vfas) is provided. More particularly, the method includes chemically or biologically pretreating a residue of algae to obtain an extract of the algae residue, filtering the extract of the algae residue and anaerobically fermenting the filtrate.
|Methods of producing 6-carbon chemicals via methyl-ester shielded carbon chain elongation|
This document describes biochemical pathways for producing adipic acid, 6-aminohexanoic acid, 6-hydroxhexanoic acid, hexamethylenediamine, caprolactam, or 1,6-hexanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a c6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the enzymes or homologs accepting methyl ester shielded dicarboxylic acid substrates..
|Glucanases, nucleic acids encoding them and methods for making and using them|
The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-d-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-d-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-d-glucans or xyloglucans and other plant material containing cellulosic parts.
|Materials and methods for high-throughput determination of genome-wide dna methylation profile|
The present invention provides materials and methods for rapid and sensitive determination of global methylation profile of genomic dna. In one embodiment, the present invention provides the fluorescence polarization (fp) based measurement of dna methylation (fpdm) assay, wherein the fpdm assay comprises restriction digestion of dna molecules using a pair of methyl-sensitive and methyl-insensitive restriction endonuclease enzymes, polymerase chain extension of digested dna molecules via the incorporation of fluorescently labeled dntp(s), and analysis via fluorescence polarization techniques..
|Biological fuel cell and methods|
A fuel cell has an anode and a cathode with anode enzyme disposed on the anode and cathode enzyme is disposed on the cathode. The anode is configured and arranged to electrooxidize an anode reductant in the presence of the anode enzyme.
|Treating inflammation with a binding system|
The teachings provided herein generally relate to site-activated binding systems that selectively increase the bioactivity of phenolic compounds at target sites. More particularly, the systems taught here include a phenolic compound bound to a reactive oxygen species, wherein the phenolic compound and the reactive oxygen species react at a target area in the presence of an oxidoreductase enzyme..
|Purine nucleoside phosphorylase as enzymatic activator of nucleoside prodrugs|
A process for inhibiting a mammalian cancerous cell or virally infected cell includes providing a trichomonas vaginalis purine nucleoside phosphorylase enzyme or a tail mutant purine nucleoside phosphorylase enzyme in proximity to the mammalian cancerous cell or the virally infected cell and exposing the enzyme to a purine nucleoside phosphorylase enzyme cleavable substrate to yield a cytotoxic purine analog. The process includes introducing to the cell a vector containing the phosphorylase enzyme, or a dna sequence coding for the same and delivering to the cell an effective amount of the substrate such as 9-(β-d-arabinofuranosyl)-2-fluoroadenine (f-araa)..
|Methods and compositions for reducing bisphenol a release|
Disclosed are methods, compositions and systems pertaining to polymer coatings that entrap enzymes, specifically enzymes capable of reducing carboxylic acids.. .
|Saliva glucose monitoring system|
A glucose sensor suitable for measuring glucose levels in human saliva is provided. Systems containing the glucose sensor and methods for making and using the sensor are also provided.
|Visual assays for coatings incorporating bioactive enzymes for catalytic functions|
Disclosed herein are materials such as a coating, comprising a lipolytic enzyme or organophosphrous compound degrading enzyme. Also disclosed herein are methods of visually detecting enzyme activity in a coating by contacting the coating with a substrate of an enzyme and a visual indicator that changes appearance upon production of a product of enzyme activity on a tack-free coating surface..
|Methods and means to modify a plant genome|
Methods and means are provided to modify in a targeted manner the genome of a plant in close proximity to an existing elite event using a double stranded dna break inducing enzyme. Also provided are plants, in particular cotton plants showing tolerance to a field dose of at least 1× of at least one hppd inhibitor, and methods for making such plants..
|Compositions, methods and related uses for cleaving modified dna|
Compositions, methods and related uses are provided relating to cleaving modified dna. For example, a set of dna fragments obtainable by enzymatic cleavage of a large dna is described where at least 50% are similarly sized and have a centrally positioned modified nucleotide.
|Modulation of meiotic recombination|
The invention provides methods of modifying the level of expression or functional activity of factors such as enzymes or other catalytic proteins or structural proteins, alone or in concert, to modify the frequency of meiotic homologous recombination involving the exchange of genetic information between non-sister chromatids from homologous maternal and paternal chromosomes. The steps at which modulation may occur include: homologous chromosome pairing, double-strand break formation; resection; strand invasion; branch migration; and resolution.
|Electrotransformation of clostridium pasteurianum|
By this invention, for the first time, a method for high-efficiency genetic transformation of the anaerobic bacterium clostridium pasteurianum is provided. Clostridium pasteurianum is a bacterium of substantial industrial importance, due to its selectivity and high productivity of the biofuel and biochemical n-butanol, and its ability to grow on a wide variety of inexpensive substrates.
|Thermostable enzymes and methods of making and using the same|
Provided herein are compositions and methods for enhancing enzyme activity, half-life and/or thermostability. Also provided herein are compositions and methods including the enhanced enzymes.
|Enzymatic antimicrobial and antifouling coatings and polymeric materials|
Disclosed herein are a coating, a textile finish, a wax, elastomer, a filler, an adhesive, or a sealant, as well as polymeric materials such as a plastic, a laminate, a composite, that includes an enzyme that degrades cell wall or cell membrane components (e.g., a lysozyme, lytic transglycosylase) alone or in combination with other enzymes such as a lipolytic enzyme, a sulfuric ester hydrolase, an organophosphorus compound degradation enzyme, or an antimicrobial peptide. Also disclosed herein are methods of retarding or preventing microbial growth on or in a coating, paint, textile finish, wax, elastomer, adhesive, sealant, filler, or a polymeric material, where such a surface material includes an enzyme that degrades cell wall or cell membrane components (e.g., a lysozyme, lytic transglycosylase)..
|Fabric and home care products|
This invention relates to fabric and home care products comprising one or more cold water proteases and processes for making and using such products. Such compositions provide improved cleaning and freshness.
|Use of secondary paraffin sulfonates for increasing the cleaning capacity of enzymes|
An improvement for boosting the cleaning capacity of one or more enzymes includes providing one or more secondary paraffin-sulfonates to a cleaning composition. The method is particularly effective for textiles at low washing temperatures..
|Dfpase enzymes from aplysia californica|
The present invention relates to isolated polypeptides having organophosphorous hydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Method for preparing a hydrocarbon|
A method for preparing a hydrocarbon comprising contacting a fatty acid substrate with at least one fatty acid reductase and at least one fatty aldehyde synthetase and at least one fatty acyl transferase, wherein the fatty acid substrate is a fatty acid, a fatty acyl-acp, or a fatty acyl-coa or a mixture of any of these, to obtain a fatty aldehyde; and contacting the fatty aldehyde with at least one aldehyde decarbonylase enzyme.. .
|Cell-free system for converting methane into fuel and chemical compounds|
The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.. .
|Methods of producing 7-carbon chemicals via c1 carbon chain elongation associated with coenzyme b synthesis|
This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a c7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the c1 elongation enzymes or homolog associated with coenzyme b biosynthesis..
|Methods of producing 7-carbon chemicals via carbon chain elongation associated with cyclohexane carboxylate synthesis|
This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a c7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the carbon chain elongation enzymes or homologs thereof associated with the cyclohexane carboxylate biosynthesis from syntrophus aciditrophicus or 2-aminoadipate lysine biosynthesis..
|Methods of producing 7-carbon chemicals via aromatic compounds|
This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a c7 aliphatic backbone substrate produced from chorismate or benzoate. These pathways, metabolic engineering and cultivation strategies described herein rely on the anaerobic benzoyl-coa degradation pathway enzymes..
Provided are biosensors, compositions comprising biosensors, and methods of using biosensors in living cells and organisms. The biosensors are able to be selectively targeted to certain regions or structures within a cell.
|Schiff-base conjugate of n, n-dibutyl-p-phenylenediamine with pyridoxal 5'-phosphate for improved homocysteine assays using pyridoxal 5'-phosphate-dependent enzymes|
A composition, method and kit for performing a two-reagent enzymatic homocysteine assay, wherein a single homocysteinase enzyme and a schiff-based conjugate of n,n-dibutyl-p-phenyldiamine (dbpda) with pyridoxal 5′-phosphate (plp) are used to measure total homocysteine in plasma or serum. The assay measures a chromophore reaction product of h2s and the dbpda released from the schiff-base conjugate in the presence of a fe+3 containing compound.
|Method and compositions for improving selective catabolysis and viability in cells of keratin surfaces|
A composition for treating keratin surfaces to stimulate selective catabolysis and improve cellular viability comprising at least one autophagy activator and at least one dna repair enzyme, and a method for improving selective catabolysis and cellular viability by treating with the composition.. .
|High concentration alpha-glucosidase compositions for the treatment of pompe disease|
The present application provides for compositions comprising high concentrations of acid α-glucosidase in combination with an active site-specific chaperone for the acid α-glucosidase, and methods for treating pompe disease in a subject in need thereof, that includes a method of administering to the subject such compositions. The present application also provides methods for increasing the in vitro and in vivo stability of an acid α-glucosidase enzyme formulation..
|Method for treating oncological diseases|
A method to treat cancer and other malignant diseases, said method comprising parenterally administering an agent which destroys blood extracellular dna into the systemic circulation of a cancer patient to slow down cancer growth. The agent is embodied in the form of a dnase enzyme and, more particularly, as a dnase i enzyme.
|Methods of reducing extravasation of inflammatory cells|
A method for modifying access of cells to extravascular spaces and regions comprising administering to a patient an enzyme that cleaves chondroitin sulfate proteoglycans is provided. It has been found that administration of an enzyme that cleaves chondroitin sulfate proteoglycans to a patient disrupts extravasation of cells from the blood stream into tissue.
|Method for enzymatic cross-linking of a protein|
A method for cross-linking albumin for use as a sealant or glue for a biological system, for example to induce hemostasis and/or prevent leakage of any other fluid from a biological tube or tissue, such as lymph for example. The cross-linked albumin may optionally and preferably be applied as part of a bandage for example.
|Pluripotent therapeutic compositions and uses thereof|
Synthetic stem cell-like tissue healing and regeneration medication with anti-inflammatory, protein synthesis, enzyme deficiency activation and genetic therapy, and anti-cancer agent derived from a series of inventions that include these products of biomolecular engineering, drug discovery from a biologic periodic table of applied biochemistry and biophysics. Tissue has a self healing effect promoting tissue healing and tissue regeneration.
|Enzyme directed assembly of particle theranostics|
Provided herein is a method for enzymatically triggered assembly of polymeric nanostructures for detection of cancer-associated enzymes in vivo. By detecting enzymatic signals associated with disease, one can sensitively determine the site, and extent of disease within a patient..