|| List of recent Electrophoresis-related patents
| Implantable high flow dual lumen multi-window vascular access port catheter|
This improved subcutaneous implantable vascular access port invention is designed to accommodate a larger catheter caliber attachment and six multi-window elongated dual lumen trough reservoir to allow 200-600 milliliters per minute of blood flow. This accommodating high flow fluid volume blood flow rate will be ideal to support functions to include but not limited to dialysis and electrophoresis.
| Automated size selection of nucleic acids|
Apparatus and methods for size selecting nucleic acid molecules having wide range of applications including the production of dna libraries for sequencing technologies. An automated high throughput system for size selection of multiple nucleic acid samples that uses imaging technique to detect the progress of a target fraction and feedback from the imaging to control electrophoresis.
| Sample analysis method and solution to be used therein|
Provided are a sample analysis method using capillary electrophoresis capable of enhancing analysis accuracy, a solution for capillary electrophoresis, and a sample analysis kit. The sample analysis method includes separating and/or detecting a substance to be analyzed in a sample through capillary electrophoresis, in which the substance to be analyzed is separated and/or detected in the presence of a ph buffer substance and a non-surfactant-type zwitterionic substance.
|Disposable capillary electrophoresis detecting device|
A disposable capillary electrophoresis detecting device includes a fixing device, a capillary electrophoresis microchip, and an electrochemical sensor microchip. The fixing device includes two chip-fixing bases having a first chip-holding cavity horizontally arranged and a second chip-holding cavity vertically arranged.
|Axial illumination for capillary electrophoresis|
System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.. .
|Method for diagnosing whether a subject is at high risk for developing atherosclerotic vascular disease|
A method for diagnosing whether a subject is at high risk for developing atherosclerotic vascular disease (asvd) is provided. The method comprises the following steps: (1) providing a specimen from a subject; (2) analyzing the specimen by using a gradient gel electrophoresis analysis; and (3) based on the result of the gel electrophoresis analysis in step (2), determining if the subject is at high risk for developing atherosclerotic vascular disease..
|Universal sample preparation system and use in an integrated analysis system|
The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract dna from a swab, amplify str loci from the dna, and analyze the amplified loci and str markers in the sample.
|"self-playing robot guitar comprising a biodegradable skin-leathern formed carcass and a biodegradable skin-leathern formed musical plectrum, and protein / amino acids"|
“self-playing robot guitar comprising a biodegradable skin-leathern formed carcass and a biodegradable skin-leathern formed musical plectrum, and structured protein/amino acids”. The invention relates to a robot having humanoid activity and extremities, having a carcass comprising a chordophone, in particular a guitar, the body of which is optionally skin-leathern formed.
|Methods and systems combining ac electroosmosis with dielectrophoresis to enhance delivery of active agents into intraoral structures|
Methods and systems for delivering an active agent into an intraoral structure are disclosed. One embodiment of a method for implementing the subject matter described herein includes generating an electrical signal that includes a first frequency, a second frequency, and a third frequency, and supplying the electrical signal to an embedded circuit contained in an intraoral delivery tray, wherein the embedded circuit includes at least one electrode that includes projections positioned proximally to a surface of an intraoral structure.
|Transilluminator base and scanner for imaging fluorescent gels|
Cassette electrophoresis systems that allow viewing of molecules during the electrophoresis run are disclosed. Cassette electrophoresis bases that reversibly engage light sources, such as light source bases are disclosed.
|Electrokinetically controlled calibrant delivery|
An electrokinetic pump can be used to deliver calibrant (“lock mass”) ions to a mass spectrometer for calibration of a mass spectrometry system. Electrokinetically controlled calibrant delivery can help to eliminate the need for the more cumbersome mechanisms that are often used for ion delivery.
|Crosslinking of swellable polymer with pei|
The invention is directed to stable and labile crosslinked water swellable polymeric microparticles that can be further gelled, methods for making same, and their various uses in the hygiene and medical arts, gel electrophoresis, packaging, agriculture, the cable industry, information technology, in the food industry, papermaking, use as flocculation aids, and the like. More particularly, the invention relates to a composition comprising expandable polymeric microparticles having labile crosslinkers and stable crosslinkers, said microparticle mixed with a fluid and an unreacted tertiary crosslinker comprising pei or other polyamine based tertiary crosslinker that is capable of further crosslinking the microparticle on degradation of the labile crosslinker and swelling of the particle, so as to form a stable gel.
|High throughput detection of fusion proteins|
A method and related microfluidic chip and kit for high throughput detection of proteins of interest contained in a sample is disclosed. The method comprises of specifically labeling fusion proteins in a complex sample with fusion tag specific fluorophores that specifically bind the fusion tags coupled to the proteins of interest, and subjecting the sample to automated capillary electrophoresis, wherein the presence of the proteins of interest in the sample is detected by fluorescence signals associated with the fusion tag specific fluorophores..
|Gel electrophoresis device for loading large sample volumes|
A device for gel electrophoresis is provided having larger wells for loading an increased sample volume with improved gel resolution. The device includes a gel cassette having a front plate and a back plate, wherein at least one plate has a stepped inner surface to create a wider opening at the top of the gel cassette, a gel matrix, and a comb with teeth having a thickness substantially equal to the spacing at the top opening of the gel cassette.
|Polyacrylamide electrophoresis gels with protection against oxygen exposure|
The detrimental effect of oxygen exposure on the formation of polyacrylamide electrophoresis gels in oxygen-permeable plastic cassettes is reduced or eliminated either by the use of an oxygen barrier material over the surfaces of the plastic walls of the cassette through which oxygen would otherwise pass into the cassette cavity, or by the incorporation of an oxygen scavenger in the plastic from which the cassette walls are made, or both.. .
|Method and apparatus for the manipulation and/or the detection of particles|
Method and apparatus for the manipulation and/or control of the position of particles using time-variable fields of force; the fields of force can be of dielectrophoresis (positive or negative), electrophoresis, electrohydrodynamic or electrowetting on dielectric, possessing a set of stable points of equilibrium for the particles.. .
|Molecular entrapment and enrichment|
Methods, structures, devices and systems are disclosed for rapid enrichment and mass transport of biomolecules (e.g., such as proteins) or other small molecules and particles using electrodeless dielectrophoresis (edep). In one aspect, a device to aggregate molecules includes a substrate that is electrically insulating, an electrically insulative material formed on the substrate and structured to form a channel to carry an electrically conducting fluid containing particles, a constriction structure formed of the electrically insulative material and located in the channel to narrow a channel dimension and forming an opening with a size in the nanometer range, and a circuit coupled to the substrate to apply an ac electric field and a dc bias electric field along the channel, in which the constriction structure is structured to magnify the applied ac electric field to produce forces that operate collectively to aggregate the particles..
|Circuit based optoelectronic tweezers|
A microfluidic optoelectronic tweezers (oet) device can comprise dielectrophoresis (dep) electrodes that can be activated and deactivated by controlling a beam of light directed onto photosensitive elements that are disposed in locations that are spaced apart from the dep electrodes. The photosensitive elements can be photodiodes, which can switch the switch mechanisms that connect the dep electrodes to a power electrode between an off state and an on state..
|Microfluidic dielectrophoresis system|
A microfluidic dielectrophoresis system includes: one supply device for a liquid medium having particles contained therein, n≧2 microfluidic, dielectrophoretically active channels, which are equipped with electrodes, lines for the fluidic connection of the supply device to the channels, for the connection of the channels to one another, and for the drainage of the medium and/or the particles from the channels, and valves for setting the flow direction of the medium in the lines, the dielectrophoretically active channels being situated and being connected by lines in such a way that they may be operated connected in parallel and in series by switching the valves in relation to the flow direction of the medium and the electrodes of the various channels are activatable independently of one another.. .
|Electrophoresis separation methods|
An improved method of separating a macromolecule by isoelectric focusing comprising subjecting the macromolecule to electrophoresis in an isoelectric-focusing medium including a substantially thiol-free reducing agent, preferably a trivalent phosphorous compound and more preferably tributyl phosphine, the improvement being the solubility and focusing of the macromolecule is enhanced compared to isoelectric focusing of the same macromolecule in a similar isoelectric-focusing medium containing a thiol-reducing agent.. .
|Capillary assembly useful as connecting capillary|
There is provided a capillary assembly suitable for connecting various components of an analytical measuring device, for example a liquid chromatograph or a capillary electrophoresis device, to each other. Specifically, this involves reinforcing the fragile tubing by the addition of peek or steel sleeves and/or embedding the tubing in an injection-molded resin such that the tubing is not exposed directly to operator handling and manipulation.
|Chip for electrophoresis and method for producing same|
A chip (10) for electrophoresis is provided, the chip including a gel (9) composed of a polymer prepared by polymerizing a monomer, and a support (1) configured to support the gel (9). In the chip (10) for electrophoresis, a surface of the support (1) in contact with the gel (9) is covered with a surface treatment compound (4) containing the monomer or a derivative of the monomer..
|Integrated affinity microcolumns and affinity capillary electrophoresis|
Device and method for detecting the presence of known or unknown toxic agents in a fluid sample. Targets in the sample are bound to releasable receptors immobilized in a reaction region of a micro- or nano-fluidic device.
|Image capture for large analyte arrays|
Analyte arrays such as solutes in a slab-shaped gel following electrophoresis, and particularly arrays that are in excess of 3 cm square and up to 25 cm square and higher, are imaged at distances of 5 cm or less by either forming sub-images of the entire array and stitching together the sub-images by computer-based stitching technology, or by using an array of thin-film photoresponsive elements that is coextensive with the analyte array to form a single image of the array.. .
|Dishwashing liquid having bleaching catalyst and protease|
In a dishwashing liquid, the cleaning performance, in particular on bleachable stains such as, for example, tea stains, is to be improved. This succeeds using a dishwashing liquid which comprises a hydrogen peroxide source, a bleaching catalyst and a protease that, in native electrophoresis on a polyacrylamide gel, has a migration distance that is longer than the migration distance of the protease as per seq id no.
|Method for forming microcapsules for electrophoresis display|
An embodiment of the invention provides a method for forming microcapsules for electrophoresis display by forming microcapsules through a complex coacervation reaction between a sulfonated styrene maleic anhydride copolymer and gelatin, wherein a mass ratio of the sulfonated styrene maleic anhydride copolymer to the gelatin is 1:10˜10:1. The method of the embodiment of the invention not only can obtain microcapsules with good sealability and stability, but also can obtain microcapsules having evenly distributed particle sizes and low cost..
|High efficiency particle separating apparatus and method|
A particle separating apparatus and method are provided, which pass a fluid sample such as blood through a filter to remove foreign matter, and separate target particles by using a moff channel, and re-separate the separated target particles through dielectrophoresis. The particle separating apparatus includes a moff (multi orifice flow fractionation) channel including a multi orifice segment through which a fluid sample passes to discharge a primarily separated material that are target particles separated from the fluid sample, through a central passage; a dielectrophoresis channel including a pair of electrodes to which ac power is applied and forming an electric field in a flow channel connected to the central passage of the moff channel to re-separate the target particles from the primarily separated material discharged from the central passage of the moff channel through dielectrophoresis..
|Negative dielectrophoresis for selective elution of immumo-bound particles|
The procedure of dielectric electrophoresis (dielectrophoresis or dep) utilizes field-polarized particles that move under the application of positive (attractive) and/or negative (repulsive) applied forces. This invention uses negative dielectric electrophoresis (negative dielectrophoresis or ndep) within a microchannel separation apparatus to make particles move (detached) or remain stationary (attached).
|Crosslinked swellable polymer|
The invention is directed to stable crosslinked water-soluble swellable polymers, methods for making same, and their various uses in the hygiene and medical arts, gel electrophoresis, packaging, agriculture, the cable industry, information technology, in the food industry, papermaking, use as flocculation aids, and the like. More particularly, the invention relates to a composition comprising expandable polymeric microparticles having labile crosslinkers and stable crosslinkers, said microparticle mixed with a fluid and an unreacted tertiary crosslinker that is capable of further crosslinking the microparticle on degradation of the labile crosslinker so as to form a stable gel.
|Recombinase polymerase amplification|
This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.
|Detection of mirna using capillary electrophoresis with laser-induced fluorescence detection|
Disclosed is a method for detecting a mirna present in a sample in trace amounts and a kit for detecting the same. According to the present invention, the mirna present in the sample in trace amounts can be quantitatively analyzed in short time.
|Methods for production of proteins|
The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies thereby facilitating rapid isolation and purification of recombinant proteins.
|Reagent applying device and reagent applying method for electrophoresis analysis|
To provide a reagent applying device and a reagent applying method for electrophoresis analysis which make it possible to apply a reagent with a simple configuration at low cost. A reagent supplying device for supplying a reagent to the surface of a gel in electrophoresis analysis includes a reagent applying tool which is a plate-shaped body.
|Microbial cell and particle selection system and method of use|
The invention comprises two key components: dielectrophoresis (dep) and reversible binding surfaces. Dep has become an important tool for trapping dielectric particles.
|Method of mutation detection in blood cell-free dna using primer extension (pe) and pcr|
A method of detecting mutation in blood cell-free dna, includes providing a serum sample, isolating dna from the serum sample, amplifying the dna by polymerase chain reaction (pcr), subjecting the pcr product to primer extension (pe), and separating the pe reaction product and identifying the mutation by gel electrophoresis. In order to improve accuracy and sensitivity, the pe reaction can be carried out by using a primer that blocks the extension of the wild or non-mutated sequence..
|Axial illumination for capillary electrophoresis|
System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.. .
|High speed, high resolution compositions, methods and kits for capillary electrophoresis|
The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer.
|Extended risk assessment panel for individualized treatment of cardiovascular disease, and methods related thereto|
Disclosed is a personalized diagnostic and treatment solution for cardiovascular disease. The invention comprises an extended cvd risk assessment panel, combining tests for traditional and new important risk markers, and methods for devising a personalized treatment plan for a patient via the use of a cvd diagnosis and treatment protocol algorithm.
|Software for the display of chromatographic separation data|
Techniques and systems for displaying chromatographic data using a graphical user interface are provided. Chromatographic separation data that represent multiple series of measurements for multiple samples can be displayed on a display device of a computer system as a series of bands, the bands being arranged to resemble output from an electrophoresis gel.
|Electrophoresis-gel-forming monomer solution, solution ejecting method, method for forming electrophoresis gel, electrophoresis gel, and electrophoresis reaction instrument|
A solution ejecting method includes a first ejection step of ejecting an electrophoresis-gel-forming monomer solution containing at least a monomer that forms a gel structure and a gel polymerization accelerator that activates a gel polymerization initiator and a second ejection step of ejecting a gel polymerization initiating solution containing the gel polymerization initiator onto the electrophoresis-gel-forming monomer solution.. .
|Plasmid-encoded neurotoxin genes in clostridium botulinum serotype a subtypes|
The present invention provides a novel isolated plasmid, wherein the plasmid is a native plasmid found in unique c. Botulinum type a strains and encode either bont/a3 or bont/a4 and bont/b.
|Methods and devices for multi-color, out-of-phase detection in electrophoresis|
The disclosure provides methods and devices for separating and detecting nucleic acid fragments labeled with a plurality of spectrally resolvable dyes using a single light source or multiple light sources. Use of a greater number of light sources increases the number of spectrally resolvable dyes that can be interrogated.
|Expanding cam lock for sealing slab gels in an electrophoresis apparatus|
An expanding cam lock for use with an electrophoresis system is disclosed herein. The cam lock allows the simultaneous use of multiple slab gel cassettes in first and second buffer core assemblies in an electrophoresis system while maintaining the necessary compressive force to create a liquid-tight seal between the anode and cathode buffer solutions.
|System for rapid high-resolution gel electrophoresis|
Electrophoretic systems, formulations and methods are described which allow a user to perform electrophoresis experiments under conditions of high voltage and with reduced run time. An electrophoretic system, formulation or method may be run at 50% higher field strength than comparable systems already in use in the art.
|Microfluidics with wirelessly powered electronic circuits|
Techniques, devices and systems are described for incorporating a printed circuit with a microfluidic device and wirelessly powering the microfluidic device. In one aspect, a microfluidic device includes a substrate with a fluidic channel to provide a path for a fluid with particles.
|Two-dimensional gel electrophoresis apparatus and method|
Two-dimensional gel electrophoresis apparatus includes an electrophoresis zone having four edges defined by a plurality of electrodes. A pair of opposed edges are defined by groups of discrete electrodes.
|High-throughput multi laser wave mixing detection methods and apparatus|
This invention relates to methods and apparatus of a combination of multi-laser wave mixing technology with diagnostic flow technologies with embodiments describing capillary electrophoresis. The unique combination of these technologies along with minute detection levels not yet been seen in the field..
|High-throughput single laser wave mixing detection methods and apparatus|
This invention relates to methods and apparatus of a combination of a single laser wave mixing technology with a diagnostic flow technologies with embodiments describing capillary electrophoresis. The unique combination of these technologies along with minute detection levels not yet been seen in the field..
|Ex-vivo multi-dimensional system for the separation and isolation of cells, vesicles, nanoparticles, and biomarkers|
Devices and techniques are described that involve a combination of multidimensional electrokinetic, dielectrophoretic, electrophoretic and fluidic forces and effects for separating cells, nanovesicles, nanoparticulates and biomarkers (dna, rna, antibodies, proteins) in high conductance (ionic) strength biological samples and buffers. In disclosed embodiments, a combination of continuous and/or pulsed dielectrophoretic (dep) forces, continuous and/or pulsed field dc electrophoretic forces, microelectrophoresis and controlled fluidics are utilized with arrays of electrodes.
|Modified electrode buffers for stain-free protein detection in electrophoresis|
Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the ph of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel.
A particle transporter based on travelling-wave dielectrophoresis is provided. The particle transporter includes a first collection electrode and a second collection electrode on a substrate.
A particle transporter based on travelling-wave dielectrophoresis is provided. The particle transporter includes a common electrode, a plurality of left switch electrodes and a plurality of right switch electrodes.
|Capillary tubes for electrophoresis|
The present invention relates to a plastic capillary tube for capillary electrophoresis, in which the plastic capillary tube has an inlet opening and an outlet opening and, furthermore, has at least one hole in the capillary tube wall and the diameter of the hole on the inside of the capillary tube wall dl(innen) lies in the range from 0.5 μm to 30 μm.. .
|Method of electrophoresing nucleic acids, method of concentrating and purifying nucleic acids, cartridge for nucleic acid electrophoresis, and method of producing cartridge for nucleic acid electrophoresis|
[solving means] a method of electrophoresis includes electrophoresing a nucleic acid in which an intercalator having an anionic functional group has been inserted. The method includes mixing a sample containing a nucleic acid, a compound having a functional group which undergoes dehydration condensation reaction with carboxyl groups included in substances contained in the sample and a condensing agent of dehydration condensation reaction; and electrophoresing the nucleic acid.