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Dna Polymerase

Dna Polymerase-related patent applications - as published by the U.S. Patent and Trademark Office (USPTO).


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Methods for assembling dna molecules
Synthetic Genomics, Inc.
June 14, 2018 - N°20180163254

The invention provides compositions and methods for assembling a dna molecule having a desired sequence. The methods involve contacting a dna polymerase, dntps, and a plurality of pairs of oligonucleotides.
Kit for together detecting multiple target nucleic acids differing from each other and detection method ...
Mizuho Medy Co., Ltd.
June 07, 2018 - N°20180155764

Provided is a kit for detecting multiple target nucleic acids capable of simultaneously amplifying and detecting multiple genes by means of one reaction vessel containing one kind of reaction solution and one kind of labels. Solution may contain first target nucleic acid (10) and second target nucleic acid (20) each of which dissociates at denaturation temperature t0.
Polymerases engineered to reduce nucleotide-independent dna binding
Omniome, Inc.
June 07, 2018 - N°20180155698

Provided are engineered dna polymerases exhibiting modified functionality, and polynucleotides encoding same. Modified features include: (1) reduced catalytic activity in the presence of magnesium ions and/or (2) reduced affinity for primed template nucleic acid molecules in the absence of cognate nucleotide, and an ability to discriminate between cognate and non-cognate nucleotides under low salt conditions.
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Dna polymerases with increased 3'-mismatch discrimination
Roche Molecular Systems, Inc.
May 24, 2018 - N°20180142220

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Pore-forming protein conjugate compositions and methods
The Trustees Of Columbia University In The City Of New York
May 17, 2018 - N°20180135121

The present disclosure relates to compositions and methods based on a fast, efficient chemical reaction for conjugating a pore-forming protein, such as α-hemolysin, to a biomolecule, such as antibodies, receptors, and enzymes, such as dna polymerase, and the use of such pore-forming conjugates in nanopore devices and methods.. .
Dna polymerases with increased 3'-mismatch discrimination
Roche Molecular Systems, Inc.
May 17, 2018 - N°20180135034

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
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Method for detecting target nucleic acid
Ngk Insulators, Ltd.
May 03, 2018 - N°20180119211

The disclosure of the present description provides a method for detecting a target nucleic acid, whereby probe hybridization can be accomplished efficiently. To that end, a target nucleic acid is amplified using a first primer having a tag sequence complementary to a detection probe pre-associated with the target nucleic acid and a first recognition sequence that recognizes a first base ...
Dna polymerases with increased 3'-mismatch discrimination
Roche Molecular Systems, Inc.
May 03, 2018 - N°20180119117

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Dna polymerases with increased 3'-mismatch discrimination
Roche Molecular Systems, Inc.
May 03, 2018 - N°20180119116

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Recombinant dna polymerase for improved incorporation of nucleotide analogues
Personal Genomics, Inc.
May 03, 2018 - N°20180119115

A composition comprising a recombinant dna polymerase and 3′-esterified nucleotide analogues is provided. The recombinant dna polymerase includes an amino acid sequence that is at least 90% homology with 9° n dna polymerase (seq id no: 1), and the recombinant dna polymerase includes at least two mutations at the positions corresponding to amino acid residues 141 and 143 of the 9° n dna ...
Methods and compositions for pcr
Life Technologies Coroporation
April 05, 2018 - N°20180094250

A modified thermostable pol b dna polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable pol b dna polymerase and a modifier reagent of formula i wherein the reaction results in a thermally reversible inactivation of the thermostable pol b dna polymerase activity and the 3′-5′ exonuclease activity, which polymerase is suitable for hot-start pcr. ...
Qualitative and absolute quantification kit for detecting hepatitis b virus cccdna
March 15, 2018 - N°20180073087

A composition for detecting hepatitis b virus cccdna includes an upstream primer having the dna sequence set forth in seq id no. 1, a downstream primer having the dna sequence set forth in seq id no.
Affenadenovirus (gorilla) or adenoviral vectors and methods of use
Genvec, Inc.
March 08, 2018 - N°20180066281

The invention provides an adenovirus or adenoviral vector characterized by comprising one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein.. .
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Materials and methods for the synthesis of error-minimized nucleic acid molecules
Synthetic Genomics, Inc.
February 22, 2018 - N°20180051280

The present invention provides materials and methods useful for error correction of nucleic acid molecules. In one embodiment of the invention, a first plurality of double-stranded nucleic acid molecules having a nucleotide mismatch are fragmented by exposure to a molecule having unidirectional mismatch endonuclease activity.
Dna polymerases with increased 3'-mismatch discrimination
Roche Molecular Systems, Inc.
February 15, 2018 - N°20180044648

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Recombinant polymerases with increased phototolerance
Pacific Biosciences Of California, Inc.
February 15, 2018 - N°20180044647

Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic ...
Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization ...
Takara Bio Usa, Inc.
February 01, 2018 - N°20180030522

The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step.
Dna polymerases with increased 3'-mismatch discrimination
Roche Molecular Systems, Inc.
January 25, 2018 - N°20180023063

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Dna polymerases with improved activity
Roche Molecular Systems, Inc.
January 11, 2018 - N°20180010107

Provided are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of primer extension methods.
Dna polymerases with improved activity
Roche Molecular Systems, Inc.
January 11, 2018 - N°20180010106

Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
Reagents and methods of pcr
Brandeis University
January 04, 2018 - N°20180002739

Modified double-stranded oligonucleotides that have terminal regions on each of their strands, that have a hybrid length of 6-50 nucleotides long, that have a melting temperature tm of at least 32° c., and that include 2-4 modifying groups, each covalently attached to a different terminal region, preferably to a terminal nucleotide, said modifying groups being polycyclic substituents that do not ...
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