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This page is updated frequently with new Dna Polymerase-related patent applications.




 Mutant dna polymerase blends and mutant dna polymerases patent thumbnailnew patent Mutant dna polymerase blends and mutant dna polymerases
A thermostable dna polymerase composition comprising at least two dna polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, dna sequencing, nucleic acid amplification and cdna synthesis..
Life Technologies Corporation


 Enzyme preparation containing thermostable dna polymerase,  producing same, and  detecting subject organism to be detected patent thumbnailEnzyme preparation containing thermostable dna polymerase, producing same, and detecting subject organism to be detected
Disclosed is a thermostable dna polymerase preparation which can illimitably reduce the risk of false positivity in the detection of a subject microorganism utilizing a gene amplification reaction and therefore enables the selective amplification of dna for detecting the subject microorganism even when the amount of the subject microorganism is small and therefore the amount of dna collected therefrom is extremely small, and can be produced at a reduced cost. Also disclosed is a method for quantifying or quantifying/identifying a subject organism to be detected rapidly, conveniently and with high sensitivity using the preparation of the present invention..
National University Corporation University Of Toyama


 Recombinant polymerases with increased phototolerance patent thumbnailRecombinant polymerases with increased phototolerance
Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like.
Pacific Biosciences Of California, Inc.


 Methods and compositions for rapid seamless dna assembly patent thumbnailMethods and compositions for rapid seamless dna assembly
Provided herein are methods for assembling dna fragments employing at least three enzymatic activities: dna polymerase, flap endonuclease, and dna ligase. Certain aspects include methods for generating closed circular dna products, e.g., plasmid vectors, by assembling various dna fragments having complementary ends that hybridize to one another.
Agilent Technologies, Inc.


 Dna polymerases with increased 3'-mismatch discrimination patent thumbnailDna polymerases with increased 3'-mismatch discrimination
Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


 Dna polymerases with increased 3'-mismatch discrimination patent thumbnailDna polymerases with increased 3'-mismatch discrimination
Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


 Dna polymerases with increased 3'-mismatch discrimination patent thumbnailDna polymerases with increased 3'-mismatch discrimination
Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


 Modified polymerase compositions, methods and kits patent thumbnailModified polymerase compositions, methods and kits
This invention relates to type i dna polymerases, for example, taq dna polymerase, and to methods and reagent kits utilizing such polymerases.. .
Brandeis University


 Integrated on chip detector and zero waveguide module structure for use in dna sequencing patent thumbnailIntegrated on chip detector and zero waveguide module structure for use in dna sequencing
A semiconductor structure for use in single molecule real time dna sequencing technology is provided. The structure includes a semiconductor substrate including a first region and an adjoining second region.
International Business Machines Corporation


 Nucleic acid amplification method using allele-specific reactive primer patent thumbnailNucleic acid amplification method using allele-specific reactive primer
The detection method using the allele-specific reactive primer (asrp) according to the present invention is a technique having a very high specificity of amplification due to the characteristics of the asrp and a proofreading dna polymerase. The detection method can effectively detect mutations (point mutation, insertion, deletion, etc.) including a single nucleotide polymorphism (snp), and can also be used to detect methylation of cpg after bisulfite treatment or to amplify and detect a target dna starting with a desired nucleotide sequence from a dna library..

Ph measurement for sequencing of dna


The present method involves sequencing by synthesis in which a template strand having an attached primer is immobilized in a small volume reaction mixture. In one embodiment, the reaction mixture is in contact with a sensitive heat sensor, which detects the heat of reaction from incorporation of a complementary base (dntp) in the presence of appropriate reagents (dna polymerase, and polymerase reaction buffer).

A the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides


Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during dna polymerase-mediated replication of dna or rna polymerase-mediated transcription of rna. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded dna or rna), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker..
Synthorx, Inc.


Mutant endonuclease v enzymes and applications thereof


Provided herein are mutant endonuclease v enzymes that are capable of nicking an inosine-containing dna sequence. Nucleic acid assays and agents that employ such mutant endonuclease v enzymes to introduce a nick into a target dna including one or more inosine, and uses a dna polymerase to generate amplicons of a target dna are also described..
General Electric Company


Recombinant reverse transcriptases


The present invention relates to a gene that encodes a hyperactive reverse transcriptase having dna polymerase activity and substantially reduced rnase h activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cdna from mrna using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase..
Applied Biosystems, Llc


Fusion polymerase and using the same


This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a dna polymerase; and (b) a heterologous sequence-specific dna binding domain. A method for copying a dna template, as well as a kit for performing the same, are also described..
New England Biolabs, Inc.


Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


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Design, synthesis and use of synthetic nucleotides comprising charge mass tags


Embodiments of the present disclosure relate generally to reporter compositions which are synthetic nucleotides that comprise nucleotides with a high charge mass moiety attached thereto via a linker molecule. The linker molecules can vary in length in part to enable the high charge mass moiety to extend out from a dna polymerase complex so that polymerization may not be influenced..
Quantumdx Group Limited


Method of determining the nucleotide sequence of oligonucleotides and dna molecules


The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection.
Life Technologies Corporation


Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


Gene expression analysis


The present invention is directed to methods and kits for gene analysis. The methods of the invention comprise the steps of providing nucleic acid; synthesis of a single-stranded dna that is complementary to said nucleic acid molecule by contacting the nucleic acid with a dna polymerase, a primer and a mixture of dntps under conditions that allow the generation of the dna, wherein the primer comprises a target-complementary region and wherein the dntp mixture comprises datp, dgtp, dctp, dttp and dutp; cleaving the dna 5′ to du sites by (i) contacting the dna with an uracil deglycosylase to generate a basic sites at positions of dutp incorporation in the dna; and (ii) contacting the dna with an apurinic/apyrimidinic (ap) endonuclease; contacting the dna comprising at its 5′-end the nucleotide sequence of the primer with a ssdna ligase to circularize the dna; and sequencing the circularized cdna.
Rheinische Friedrich-wilhelms-universität Bonn


Affenadenovirus (gorilla) or adenoviral vectors and methods of use


The invention provides an adenovirus or adenoviral vector characterized by comprising one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein.. .
Genvec, Inc.


Polypeptides having nucleic acid binding activity


Polynucleotides having nucleic acid binding activity are provided. Methods of stabilizing a nucleic acid duplex are provided.
Applied Biosystems, Llc


Polymerases


Modified dna polymerases have an affinity for dna such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate dna templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-dna complexes in each reaction cycle.
Illumina Cambridge Limited


Mutant dna polymerases and methods of use


The present invention provides mutant dna polymerases, polynucleotides encoding the polymerases, cassettes and vectors including such polynucleotides, and cells containing the polymerases, polynucleotides, cassettes, and/or vectors of the invention. The present invention also provides methods for synthesizing polynucleotides and kits including a dna polymerase of the invention..
Applied Biosystems, Llc


Dna polymerases and mutants thereof


The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may possess both a dna-dependent dna polymerase activity and an rna-dependent dna polymerase activity, i.e., a reverse transcriptase activity.

Compositions and methods for rt-pcr


The present invention relates to compositions and methods having propylene glycol and dna polymerase for facilitating the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of rna molecules, and for increasing the detection sensitivity and reliability through generation of secure cdna molecules prior to gene-specific primer dependent amplification. The reagent mixture comprises a ready to use reagent solution, wherein the solution comprises: (a) propylene glycol in a concentration between about 20% and about 50%; (b) a viral reverse transcriptase; and (c) at least one dna polymerases, in a buffer suitable for use in a reverse transcription reaction, wherein the buffer comprises a co-factor metal ion and nucleoside triphosphates..

Soy gene cluster regions and methods of use


Methods for conveying pathogen resistance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing pathogen resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with pathogen resistance.
Syngenta Participations Ag


Characterization of thermostable dna polymerase


Methods detecting covalent lysine modifications in dna polymerases are provided. These methods are particularly useful in determining the extent and location of a lysine modification in a dna polymerase..
Roche Molecular Systems, Inc.


Recombinase polymerase amplification


This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.
Alere San Diego, Inc.


Methods for measuring polymerase activity useful for sensitive, quantitative measurements of any polymerase extension activity and for determining the presence of viable cells


A novel, highly sensitive, quantitative and rapid dpe-pcr assay is disclosed that can be used to enumerate prokaryotic cells when presenting a purified or selected cell type and that has the capability to reproducibly measure dna polymerase extension activity from less than 10 cfu of bacteria via coupling to bead lysis. Also disclosed is the potential for the dpe-pcr assay of the invention to universally detect microbes by testing a panel of microorganisms comprised of gram-negative bacteria, gram-positive bacteria and candida species.
Zeus Scientific, Inc.


Markers associated with soybean rust resistance and methods of use therefor


Methods for conveying soybean rust (sbr) resistance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing sbr resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with sbr resistance.
Syngenta Participations Ag


Rna microchip detection using nanoparticle-assisted signal amplification


Disclosed are methods and materials for detecting rna in a sample. In some forms, the method involves (a) bringing into contact the sample and a probe array, (b) bringing into contact the probe array and a ribonuclease specific for rna/dna hybrids (such as rnase h), (c) bringing into contact the probe array, labeled nucleotides, and a nucleic acid polymerase capable of extending a rna strand using a dna template and capable of incorporating the labeled nucleotides in the extension from the rna strand (such as klenow fragment dna polymerase), and (d) detecting the labeled nucleotides in the extended nucleic acid strand.
Georgia State University Research Foundation, Inc.


Normalization of polymerase activity


Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of dna polymerases, such as taq dna polymerase.. .
Exact Sciences Corporation


Recombinase polymerase amplification


This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes.
Alere San Diego, Inc.


Methods and compositions for pcr


A modified thermostable pol b dna polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable pol b dna polymerase and a modifier reagent of formula i wherein the reaction results in a thermally reversible inactivation of the thermostable pol b dna polymerase activity and the 3′-5′ exonuclease activity, which polymerase is suitable for hot-start pcr. Also disclosed are the method for the modification, a polynucleic acid amplification method and pcr reaction mixture and kit comprising the modified thermostable pol b dna polymerase..
Life Technologies Corporation


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Dna polymerases and related methods


Disclosed are mutant dna polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


Enzymes resistant to photodamage


Provided are compositions comprising modified dna polymerases that exhibit improved photostability compared to the parental polymerases from which they were derived. Provided are methods for generating enzymes, such as dna polymerases, with the aforementioned phenotype.
Pacific Biosciences Of California, Inc.


Reagents and kit compositions for single-cell whole genome amplification


The present invention provides reagents, kits, and methods for single-cell whole genome amplification using phi 29 dna polymerase.. .
Fluidigm Corporation


Dna polymerases with improved activity


Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


Use of taq polymerase mutant enzymes for nucleic acid amplification in the presence of pcr inhibitors


The present invention generally relates to detection of a target nucleic acid in standard pcr, real-time pcr, rt pcr, and real-time rt pcr. One aspect of the invention provides mutant dna polymerase enzymes that are resistant to pcr inhibitors, such as dye, blood, and soil.
Dna Polymerase Technology Inc.


Nucleic acid detection using probes


The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature tm2, comprising a sequence segment that complementary to the regulatory sequence and a tail segment that does not hybridize to the probe nucleotide when the sequence segment and the regulatory sequence are annealed, amplifying the tagged target nucleic acid sequence in a pcr amplification reaction using the probe oligonucleotide as a primer, and using a dna polymerase with high strand displacement activity and low 5′-nuclease activity, and detecting the amplification product; wherein tm1 and tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.. .
Fluidigm Corporation


Transposon end compositions and methods for modifying nucleic acids


The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target dna in vitro, then using a dna polymerase for generating 5′- and 3′-tagged single-stranded dna fragments without performing a pcr amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged dna fragments for use in a variety of processes, including processes for metagenomic analysis of dna in environmental samples, copy number variation (cnv) analysis of dna, and comparative genomic sequencing (cgs), including massively parallel dna sequencing (so-called “next generation sequencing.).
Epicentre Technologies Corporation


Production of closed linear dna


An in vitro process for the production of closed linear deoxyribonucleic acid (dna) comprises (a) contacting a dna template comprising at least one protelomerase target sequence with at least one dna polymerase in the presence of one or more primers under conditions promoting amplification of the template; and (b) contacting amplified dna produced in (a) with at least one protelomerase under conditions promoting production of closed linear dna. A kit provides components necessary in the process..

Reagents and methods of pcr


Modified double-stranded oligonucleotides that have terminal regions on each of their strands, that have a hybrid length of 6-50 nucleotides long, that have a melting temperature tm of at least 32° c., and that include 2-4 modifying groups, each covalently attached to a different terminal region, preferably to a terminal nucleotide, said modifying groups being polycyclic substituents that do not have bulky portions that are non-planar, said modified oligonucleotide being capable of binding to the 5′ exonuclease domains of dna polymerases and, when included in a pcr or other primer-dependent dna amplification reaction at a concentration, generally not more than 2000 nm, that is effective for at least one of the functions of suppressing mispriming, increasing polymerase selectivity against 3′ terminal mismatches. Increasing polymerase selectivity against at-rich 3′ ends, reducing scatter among replicates, suppressing polymerase 5′ exonuclease activity, and inhibiting polymerase activity; as well as amplification reaction mixtures containing such modified double-stranded oligonucleotides, and amplification reactions, amplification assays and kits that include such modified double-stranded oligonucleotides..
Brandeis University


Dna polymerases with improved activity


Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.

Recombinase polymerase amplification


This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.

Method for competitive allele-specific cdna synthesis and differential amplification of the cdna products


The present invention provides a method for the detection of the presence of rna variants comprising the step of performing a competitive cdna synthesis comprising a first primer specific to a first rna variant, a second primer specific to a second rna variant, an rna-dependent dna polymerase, and rna from said sample as a template, wherein said first primer and said second primer comprise an allele-specific nucleotide portion, a target-specific sequence and tag units with a common sequence and/or a discriminating sequence so that the sequence of said tag units is not complementary to said first or second rna variant, wherein each of the cdna products obtained from said competitive cdna synthesis consists of the sequence of only one primer extended by the sequence complementary to one of the target rna variants.. .

Isothermal amplification under low salt condition


Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a dna polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dntp) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (tm) of the primer at least 10° c.
General Electric Company


New dna polymerases with increased substrate scope


The present invention relates to dna polymerases displaying increased substrate scope such as improved reverse transcriptase and dna polymerase activities, as well as improved activities for incorporating and extending modified nucleotides. In particular, the present invention relates to dna polymerases derived from wild-type thermus aquaticus (taq) polymerase, comprising the mutations s515r, i638f, and m747k with regard to the wild-type amino acid sequence.
Universital Konstanz


Design, synthesis and use of synthetic nucleotides comprising charge mass tags


Embodiments of the present disclosure relate generally to reporter compositions which are synthetic nucleotides that comprise nucleotides with a high charge mass moiety attached thereto via a linker molecule. The linker molecules can vary in length in part to enable the high charge mass moiety to extend out from a dna polymerase complex so that polymerization may not be influenced..
Quantumdx Group Limited


Recombinase polymerase amplification reagents and kits


This disclosure describes kits, reagents and methods for recombinase polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed kits, reagents and methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods..
Alere San Diego Inc.


Detection of an amplification reaction product using ph-sensitive dyes


Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or pcr amplification reactions to completion using ph-sensitive dyes that are either colored or fluorescent.
New England Biolabs, Inc.


Recombinant polymerases for improved single molecule sequencing


Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like.
Pacific Biosciences Of California, Inc.


Dna polymerases having improved labeled nucleotide incorporation properties


In addition to providing novel mutant dna polymerases, the invention also provides polynucleotides encoding the subject mutant dna polymerases. The polynucleotides provided may comprise expression vectors for the recombinant production of the mutant polymerases.

Thermostable type-a dna polymerase mutant with increased resistance to inhibitors in blood


The invention provides mutants of dna polymerases having an enhanced resistance to inhibitors of dna polymerase activity. The mutant polymerases are well suited for pcr amplification of targets in samples that contain inhibitors of wild-type polymerases..
Agilent Technologies, Inc.


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Dna polymerases and related methods


Disclosed are mutant dna polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


Recombinant polymerases with increased phototolerance


Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like.
Pacific Biosciences Of California


Modified dna polymerase


The modified dna polymerase comprises modifications of at least two amino acids selected from the group consisting of amino acids corresponding to y7, p36, and v93 in seq id no: 1, in the amino acid sequence represented by any one of seq id nos: 1 to 10.. .

Random-primed transcriptase in-vitro transcription rna amplification


A random-primed reverse transcriptase-in vitro transcription method of linearly amplifying rna is provided. According to the methods of the invention, source rna (or other single-stranded nucleic acid), preferably, mrna, is converted to double-stranded cdna using two random primers, one of which comprises a rna polymerase promoter sequence (“promoter-primer”), to yield a double-stranded cdna that comprises a rna polymerase promoter that is recognized by a rna polymerase.
Life Technologies Corporation


Dna polymerases with improved activity


Disclosed are dna polymerases having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of rt and polymerase inhibitors relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


Dna polymerase mutants having enhanced template discrimination activity


This invention relates to mutant dna polymerases having an enhanced template discrimination activity compared with the corresponding unmodified dna polymerase counterparts, wherein the amino acid sequence of the mutant dna polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified taq dna polymerase.. .
Integrated Dna Technologies, Inc.


Dna polymerases with improved activity


Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


Oligonucleotide inhibitor of dna polymerases


The invention comprises a reversible oligonucleotide inhibitor of nucleic acid polymerases. Methods of designing said inhibitors and using said inhibitors in amplification and detection of nucleic acid, particularly detection of rna by rt-pcr are also disclosed..
Roche Molecular Systems, Inc.


Nucleic acid amplification reactor


Provided is a nucleic acid amplification reactor that can easily perform a nucleic acid amplification reaction. A nucleic acid amplification reactor 1 includes a reaction chamber 20 to which a thermoplastic hydrogel 50 is applied.

Dual enzymatic amplification


Provided are methods for validating the presence and character of genomic mutations, particularly single nucleotide polymorphisms (snps), by parallel amplification of a portion or the whole genome with at least two different dna polymerases.. .
Cynvenio Biosystems, Inc.


Mutant neq hs dna polymerase derived from nanoarchaeum equitans and its application to hot-start pcr


A dna polymerase (neq dna polymerase) derived from nanoarchaeum equitans is split into neq l and neq s fragments, each of which contains inteins. A neq hot-start (hs) dna polymerase in which the inteins of the neq l and neq s fragments are linked with each other is provided in the form of a precursor of neq dna polymerase.
Research & Business Foundation Sungkyunkwan University


Adenoviral vector-based malaria vaccine


The invention provides an adenovirus or adenoviral vector characterized by comprising a nucleic acid sequence encoding one or more plasmodium antigens and one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein, as well as a method of inducing an immune response against plasmodium falciparum in a mammal by administering the adenovirus or adenoviral vector to the mammal.. .
Ganvec, Inc.


Novel dna polymerases


Novel proteins having dna polymerase are described which have utility in amplification reactions and have improved properties over bst polymerase such as for example enhanced reverse transcriptase activity.. .
New England Biolabs, Inc.


Method and predicting susceptibility to a developmental disorder


A nucleotide sequence signal amplification composition that includes an isolated, synthetic nucleotide sequence of greater than 7 nucleotides. The sequence is a fragment of seq id no:3 and further comprising a t nucleotide at position 1438 of seq id no:3 and one or more primers that bind to the synthetic nucleotide sequence, a thermostable dna polymerase, restriction enzyme, or a combination thereof..
Centre For Addiction And Mental Health


Nucleic acid-free thermostable enzymes and methods of production thereof


The present invention provides thermostable enzymes, such as dna polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (pcr)..
Life Technologies Corporation


Enzyme composition for dna end repair, adenylation, phosphorylation


Enzyme compositions and their method of use that provide ready-to-use master mixtures. The compositions comprise a modified thermophilic dna polymerase lacking 5′-3′ and 3′-5′ exonuclease activity premixed with t4 dna polymerase, klenow fragment and t4 polynucleotide kinase and all other necessary components, including reaction buffer and nucleoside triphosphates, required to perform dna blunting, phosphorylation, and single nucleotide extension reactions in one tube and in two steps.
Thermo Fisher Scientific Baltics Uab


Isothermal amplification using oligocation-conjugated primer sequences


Provided herein are methods and kits for isothermal nucleic acid amplifications that use an oligocation-oligonucleotide conjugate primer for amplifying a target nucleic acid to generate amplicons. Isothermal dna amplification methods that employ a strand displacing dna polymerase and polyamine-oligonucleotide conjugate primer are also provided..
General Electric Company


Modified epstein-barr virus dna polymerase and methods for isothermal dna amplification


Modified epstein barr virus dna polymerase for use in nucleic acid amplification, including isothermal nucleic acid amplification, in vitro are provided. Methods using and kits comprising epstein barr virus dna polymerase and its variants of this invention for nucleic acid amplification in vitro, including isothermal dna amplification, are also provided..
University Of Connecticut


Methods and compositions for inhibition of polymerase


The present invention is directed to methods and compositions for inhibition of viral nucleic acid polymerases, such as rna and dna polymerases, and methods and compositions that are useful for treating viral infections in subjects. The methods comprise administering to the subject a therapeutically effective amount of a compound of formula i, or a pharmaceutically acceptable salt or hydrate thereof, or a composition comprising a compound of formula i, or a pharmaceutically acceptable salt or hydrate thereof, and a pharmaceutically acceptable carrier.
Biocryst Pharmaceuticals, Inc.


Stable compositions for nucleic acid amplification and sequencing


The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable dna polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing.
Life Technologies Corporation


Method for detecting pyrophosphoric acid using support having boronic acid group immobilized thereon


An object of the present invention is to provide a method capable of easily and directly performing dna sequencing by directly detecting pyrophosphoric acid released by an enzymatic reaction, particularly with a dna polymerase or a dna ligase. The present invention provides a method and a device for detecting pyrophosphoric acid by measuring an electrical change occurring when a boronic acid group immobilized on an electrically conductive support reversibly binds to pyrophosphoric acid..
Hitachi, Ltd.


Composition for hot-start reverse transcription reaction or hot-start reverse transcription polymerase chain reaction


A composition for hot-start reverse transcription reaction and a composition for reverse transcription pcr are disclosed. The composition is obtained by adding pyrophosphate and pyrophosphatase to an aqueous solution containing reaction buffer solution, mgcl2, four kinds of dntps, and reverse transcription polymerase in a single reaction tube.
Bioneer Corporation


Hyperthermophilic polymerase enabled proximity extension assay


The present invention relates to a proximity probe based detection assay (“proximity assay”) for an analyte in a sample, specifically a proximity probe extension assay (pea), an in particular to an improvement in the method to reduce non-specific “background” signals, wherein the improvement comprises the use in such assays of a hyperthermophilic polymerase, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) extending the 3′ end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the extension reaction comprises increasing the temperature of assay above room temperature and uses a polymerase enzyme which is characterised as having less than 20% of its maximal enzyme activity at 40° c. And having less than 10% of its maximal enzyme activity at 25° c., wherein the optimum temperature for maximal activity of the polymerase is more than 40° c.
Olink Ab


Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)


The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (rt-pcr). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step rt-pcr procedure using combinations of reverse transcriptase and thermostable dna polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin.
Life Technologies Corporation


Dna polymerase activity assays and methods enabling determination of viable microbes


A method for performing a diagnostic assay for the detection of the presence or amount of a microorganism within a sample matrix containing active dna polymerase, is disclosed. The method utilizes the measurement of dna polymerase extension activity, wherein the assay comprises the steps of incubating dna polymerase in the sample matrix with a selected suitable substrate, and performing pcr cycling and detection via the use of a selected suitable nucleic acid probe, thereby to detect endogenous dna polymerase extension activity in the sample matrix as an indication of the presence or amount of said microorganism..
Zeus Scientific, Inc.


Methods for genotyping


Novel methods and kits are disclosed for reducing the complexity of a nucleic acid sample to interrogate a collection of target sequences, for example, to discriminating between alleles at polymorphic positions in a genome. Complexity reduction can be accomplished by extension of a capture probes followed by amplification of the extended capture probe using common primers.
Affymetrix, Inc.


Ssb-polymerase fusion proteins


Fusion proteins comprising a single strand dna binding protein and a nucleic acid polymerase (e.g. dna polymerase or reverse transcriptase).
Life Technologies Corporation


Isothermal amplification systems and methods


The present invention relates to systems and methods for performing isothermal amplification reactions, in particular, denaturation methods for use in isothermal amplification reactions. An exemplary method may comprise: a) contacting a target nucleic acid with an electrode, wherein the electrode surface has a plurality of first and optionally second nucleic acid primers immobilized thereon, and wherein a target nucleic acid hybridizes to at least one of said first and second nucleic acid primers; b) extending at least one of the first and second primers using a dna polymerase to form extended target nucleic acids; c) applying positive electrical bias to the electrode such that the extended target nucleic acids anneal to one of the first and second primers; d) extending the target nucleic acid with a dna polymerase to form amplified target nucleic acid; e) reversing the electrical bias such that the amplified target nucleic acid is denatured from the surface..

Novel dna polymerase


Provided are: a dna polymerase comprising: an amino acid sequence modified from the amino acid sequence of seq id no: 12, which has a substitution of arginine at position 651 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid; and a dna polymerase comprising an amino acid sequence modified from the amino acid sequence of seq id no: 14, which has a substitution of proline at position 653 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid.. .

Method for improving nucleic acid synthesis reaction


Provided are the following: a method, for improving reactivity of a nucleic acid synthesis reaction, comprising a step for adding an ω-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising dna polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an ω-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ω-amino acid.. .

Chimeric dna polymerases


The present invention provides, among other things, chimeric dna polymerases containing heterologous domains having sequences derived from at least two dna polymerases that have at least one distinct functional characteristics (e.g., elongation rate, processivity, error rate or fidelity, salt tolerance or resistance) and methods of making and using the same. In some embodiments, the present invention can combine desired functional characteristics (e.g., high processivity; high elongation rate; thermostability; resistance to salt, pcr additives (e.g., pcr enhancers) and other impurities; and high fidelity) of different dna polymerases in a chimeric polymerase..

Polymerase driven nesa


The present invention relates to a novel method for detecting a target polynucleotide having a target sequence, comprising (a) exposing the target polynucleotide to an initiating oligonucleotide; (b) extending the initiating oligonucleotide with an extended sequence complementary to the target sequence; (c) ligating the initiating oligonucleotide sequence with the extended sequence to form a circular oligonucleotide having a nicking endonuclease (ne) recognition/cutting sequence; (d) exposing the circular oligonucleotide to a dna polymerase and a dna synthesis primer to synthesize dna having a ne recognition sequence; (e) exposing the synthesized dna to a probe having the ne recognition/cutting sequence to form a double stranded dna having a full ne site; (f) exposing the double stranded dna to a nicking endonuclease (ne) to cleave the probe; and (g) detecting the cleaved probe. The presence of the cleaved probe indicates the presence of the target polynucleotide..

Nucleic acid amplification method


A nucleic acid amplification method includes ligating a double-stranded adapter (20) containing adapter dna strands capable of forming a folded structure to a double-stranded dna (1, 2) containing a target dna sequence (1) to prepare a cyclic dna template composed of double-stranded dna containing a nick (5). A 3′-end elongation reaction is performed using a strand-displacement dna polymerase from the nick (5) as an origin, thereby producing a concatemer (29) in which a plurality of the target dna sequences (1) and the adapter dna strands capable of forming the folded structure are linked in series as a single-stranded dna.

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

Method of determining the nucleotide sequence of oligonucleotides and dna molecules


The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection.



Dna Polymerase topics:
  • Dna Polymerase
  • Polymerase
  • Nucleic Acid
  • Nucleotide
  • Recombinant
  • Amplification
  • Sequencing
  • Exonuclease
  • Reverse Transcriptase
  • Dna Molecule
  • Isothermal
  • Amino Acid
  • Oligonucleotide
  • Endonuclease
  • Polynucleotide


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