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Dna Polymerase patents



      

This page is updated frequently with new Dna Polymerase-related patent applications.




Date/App# patent app List of recent Dna Polymerase-related patents
04/28/16
20160115461 
 Polymerases patent thumbnailPolymerases
Modified dna polymerases have an affinity for dna such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate dna templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-dna complexes in each reaction cycle.
Illumina Cambridge Limited


04/28/16
20160115460 
 Mutant dna polymerases and methods of use patent thumbnailMutant dna polymerases and methods of use
The present invention provides mutant dna polymerases, polynucleotides encoding the polymerases, cassettes and vectors including such polynucleotides, and cells containing the polymerases, polynucleotides, cassettes, and/or vectors of the invention. The present invention also provides methods for synthesizing polynucleotides and kits including a dna polymerase of the invention..
Applied Biosystems, Llc


04/21/16
20160108381 
 Dna polymerases and mutants thereof patent thumbnailDna polymerases and mutants thereof
The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may possess both a dna-dependent dna polymerase activity and an rna-dependent dna polymerase activity, i.e., a reverse transcriptase activity.

04/07/16
20160097086 
 Compositions and methods for rt-pcr patent thumbnailCompositions and methods for rt-pcr
The present invention relates to compositions and methods having propylene glycol and dna polymerase for facilitating the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of rna molecules, and for increasing the detection sensitivity and reliability through generation of secure cdna molecules prior to gene-specific primer dependent amplification. The reagent mixture comprises a ready to use reagent solution, wherein the solution comprises: (a) propylene glycol in a concentration between about 20% and about 50%; (b) a viral reverse transcriptase; and (c) at least one dna polymerases, in a buffer suitable for use in a reverse transcription reaction, wherein the buffer comprises a co-factor metal ion and nucleoside triphosphates..

04/07/16
20160097058 
 Soy gene cluster regions and methods of use patent thumbnailSoy gene cluster regions and methods of use
Methods for conveying pathogen resistance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing pathogen resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with pathogen resistance.
Syngenta Participations Ag


03/31/16
20160090618 
 Characterization of thermostable dna polymerase patent thumbnailCharacterization of thermostable dna polymerase
Methods detecting covalent lysine modifications in dna polymerases are provided. These methods are particularly useful in determining the extent and location of a lysine modification in a dna polymerase..
Roche Molecular Systems, Inc.


03/24/16
20160083780 
 Recombinase polymerase amplification patent thumbnailRecombinase polymerase amplification
This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.
Alere San Diego, Inc.


03/24/16
20160083776 
 Methods for measuring polymerase activity useful for sensitive, quantitative measurements of any polymerase extension activity and for determining the presence of viable cells patent thumbnailMethods for measuring polymerase activity useful for sensitive, quantitative measurements of any polymerase extension activity and for determining the presence of viable cells
A novel, highly sensitive, quantitative and rapid dpe-pcr assay is disclosed that can be used to enumerate prokaryotic cells when presenting a purified or selected cell type and that has the capability to reproducibly measure dna polymerase extension activity from less than 10 cfu of bacteria via coupling to bead lysis. Also disclosed is the potential for the dpe-pcr assay of the invention to universally detect microbes by testing a panel of microorganisms comprised of gram-negative bacteria, gram-positive bacteria and candida species.
Zeus Scientific, Inc.


03/17/16
20160073598 
 Markers associated with soybean rust resistance and methods of use therefor patent thumbnailMarkers associated with soybean rust resistance and methods of use therefor
Methods for conveying soybean rust (sbr) resistance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing sbr resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with sbr resistance.
Syngenta Participations Ag


03/10/16
20160068894 
 Rna microchip detection using nanoparticle-assisted signal amplification patent thumbnailRna microchip detection using nanoparticle-assisted signal amplification
Disclosed are methods and materials for detecting rna in a sample. In some forms, the method involves (a) bringing into contact the sample and a probe array, (b) bringing into contact the probe array and a ribonuclease specific for rna/dna hybrids (such as rnase h), (c) bringing into contact the probe array, labeled nucleotides, and a nucleic acid polymerase capable of extending a rna strand using a dna template and capable of incorporating the labeled nucleotides in the extension from the rna strand (such as klenow fragment dna polymerase), and (d) detecting the labeled nucleotides in the extended nucleic acid strand.
Georgia State University Research Foundation, Inc.


03/03/16
20160060683 

Normalization of polymerase activity


Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of dna polymerases, such as taq dna polymerase.. .
Exact Sciences Corporation


02/04/16
20160032374 

Recombinase polymerase amplification


This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes.
Alere San Diego, Inc.


02/04/16
20160032262 

Methods and compositions for pcr


A modified thermostable pol b dna polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable pol b dna polymerase and a modifier reagent of formula i wherein the reaction results in a thermally reversible inactivation of the thermostable pol b dna polymerase activity and the 3′-5′ exonuclease activity, which polymerase is suitable for hot-start pcr. Also disclosed are the method for the modification, a polynucleic acid amplification method and pcr reaction mixture and kit comprising the modified thermostable pol b dna polymerase..
Life Technologies Corporation


01/28/16
20160024548 

Dna polymerases and related methods


Disclosed are mutant dna polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


01/21/16
20160017413 

Enzymes resistant to photodamage


Provided are compositions comprising modified dna polymerases that exhibit improved photostability compared to the parental polymerases from which they were derived. Provided are methods for generating enzymes, such as dna polymerases, with the aforementioned phenotype.
Pacific Biosciences Of California, Inc.


01/21/16
20160017395 

Reagents and kit compositions for single-cell whole genome amplification


The present invention provides reagents, kits, and methods for single-cell whole genome amplification using phi 29 dna polymerase.. .
Fluidigm Corporation


01/07/16
20160002614 

Dna polymerases with improved activity


Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


12/24/15
20150368624 

Use of taq polymerase mutant enzymes for nucleic acid amplification in the presence of pcr inhibitors


The present invention generally relates to detection of a target nucleic acid in standard pcr, real-time pcr, rt pcr, and real-time rt pcr. One aspect of the invention provides mutant dna polymerase enzymes that are resistant to pcr inhibitors, such as dye, blood, and soil.
Dna Polymerase Technology Inc.


12/17/15
20150361486 

Nucleic acid detection using probes


The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature tm2, comprising a sequence segment that complementary to the regulatory sequence and a tail segment that does not hybridize to the probe nucleotide when the sequence segment and the regulatory sequence are annealed, amplifying the tagged target nucleic acid sequence in a pcr amplification reaction using the probe oligonucleotide as a primer, and using a dna polymerase with high strand displacement activity and low 5′-nuclease activity, and detecting the amplification product; wherein tm1 and tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.. .
Fluidigm Corporation


12/10/15
20150353925 

Transposon end compositions and methods for modifying nucleic acids


The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target dna in vitro, then using a dna polymerase for generating 5′- and 3′-tagged single-stranded dna fragments without performing a pcr amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged dna fragments for use in a variety of processes, including processes for metagenomic analysis of dna in environmental samples, copy number variation (cnv) analysis of dna, and comparative genomic sequencing (cgs), including massively parallel dna sequencing (so-called “next generation sequencing.).
Epicentre Technologies Corporation


11/19/15
20150329902 

Production of closed linear dna


An in vitro process for the production of closed linear deoxyribonucleic acid (dna) comprises (a) contacting a dna template comprising at least one protelomerase target sequence with at least one dna polymerase in the presence of one or more primers under conditions promoting amplification of the template; and (b) contacting amplified dna produced in (a) with at least one protelomerase under conditions promoting production of closed linear dna. A kit provides components necessary in the process..

11/12/15
20150322503 

Reagents and methods of pcr


Modified double-stranded oligonucleotides that have terminal regions on each of their strands, that have a hybrid length of 6-50 nucleotides long, that have a melting temperature tm of at least 32° c., and that include 2-4 modifying groups, each covalently attached to a different terminal region, preferably to a terminal nucleotide, said modifying groups being polycyclic substituents that do not have bulky portions that are non-planar, said modified oligonucleotide being capable of binding to the 5′ exonuclease domains of dna polymerases and, when included in a pcr or other primer-dependent dna amplification reaction at a concentration, generally not more than 2000 nm, that is effective for at least one of the functions of suppressing mispriming, increasing polymerase selectivity against 3′ terminal mismatches. Increasing polymerase selectivity against at-rich 3′ ends, reducing scatter among replicates, suppressing polymerase 5′ exonuclease activity, and inhibiting polymerase activity; as well as amplification reaction mixtures containing such modified double-stranded oligonucleotides, and amplification reactions, amplification assays and kits that include such modified double-stranded oligonucleotides..
Brandeis University


10/08/15
20150284696 

Dna polymerases with improved activity


Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.

10/08/15
20150284695 

Recombinase polymerase amplification


This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.

10/01/15
20150275286 

Method for competitive allele-specific cdna synthesis and differential amplification of the cdna products


The present invention provides a method for the detection of the presence of rna variants comprising the step of performing a competitive cdna synthesis comprising a first primer specific to a first rna variant, a second primer specific to a second rna variant, an rna-dependent dna polymerase, and rna from said sample as a template, wherein said first primer and said second primer comprise an allele-specific nucleotide portion, a target-specific sequence and tag units with a common sequence and/or a discriminating sequence so that the sequence of said tag units is not complementary to said first or second rna variant, wherein each of the cdna products obtained from said competitive cdna synthesis consists of the sequence of only one primer extended by the sequence complementary to one of the target rna variants.. .

10/01/15
20150275282 

Isothermal amplification under low salt condition


Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a dna polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dntp) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (tm) of the primer at least 10° c.
General Electric Company


09/24/15
20150267182 

New dna polymerases with increased substrate scope


The present invention relates to dna polymerases displaying increased substrate scope such as improved reverse transcriptase and dna polymerase activities, as well as improved activities for incorporating and extending modified nucleotides. In particular, the present invention relates to dna polymerases derived from wild-type thermus aquaticus (taq) polymerase, comprising the mutations s515r, i638f, and m747k with regard to the wild-type amino acid sequence.
Universital Konstanz


09/17/15
20150259731 

Design, synthesis and use of synthetic nucleotides comprising charge mass tags


Embodiments of the present disclosure relate generally to reporter compositions which are synthetic nucleotides that comprise nucleotides with a high charge mass moiety attached thereto via a linker molecule. The linker molecules can vary in length in part to enable the high charge mass moiety to extend out from a dna polymerase complex so that polymerization may not be influenced..
Quantumdx Group Limited


08/27/15
20150240298 

Recombinase polymerase amplification reagents and kits


This disclosure describes kits, reagents and methods for recombinase polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed kits, reagents and methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods..
Alere San Diego Inc.


08/27/15
20150240293 

Detection of an amplification reaction product using ph-sensitive dyes


Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or pcr amplification reactions to completion using ph-sensitive dyes that are either colored or fluorescent.
New England Biolabs, Inc.


08/27/15
20150240221 

Recombinant polymerases for improved single molecule sequencing


Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like.
Pacific Biosciences Of California, Inc.


08/20/15
20150232822 

Dna polymerases having improved labeled nucleotide incorporation properties


In addition to providing novel mutant dna polymerases, the invention also provides polynucleotides encoding the subject mutant dna polymerases. The polynucleotides provided may comprise expression vectors for the recombinant production of the mutant polymerases.

08/20/15
20150232821 

Thermostable type-a dna polymerase mutant with increased resistance to inhibitors in blood


The invention provides mutants of dna polymerases having an enhanced resistance to inhibitors of dna polymerase activity. The mutant polymerases are well suited for pcr amplification of targets in samples that contain inhibitors of wild-type polymerases..
Agilent Technologies, Inc.


08/06/15
20150218537 

Dna polymerases and related methods


Disclosed are mutant dna polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


08/06/15
20150218535 

Recombinant polymerases with increased phototolerance


Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like.
Pacific Biosciences Of California


08/06/15
20150218534 

Modified dna polymerase


The modified dna polymerase comprises modifications of at least two amino acids selected from the group consisting of amino acids corresponding to y7, p36, and v93 in seq id no: 1, in the amino acid sequence represented by any one of seq id nos: 1 to 10.. .

07/09/15
20150191781 

Random-primed transcriptase in-vitro transcription rna amplification


A random-primed reverse transcriptase-in vitro transcription method of linearly amplifying rna is provided. According to the methods of the invention, source rna (or other single-stranded nucleic acid), preferably, mrna, is converted to double-stranded cdna using two random primers, one of which comprises a rna polymerase promoter sequence (“promoter-primer”), to yield a double-stranded cdna that comprises a rna polymerase promoter that is recognized by a rna polymerase.
Life Technologies Corporation


07/09/15
20150191708 

Dna polymerases with improved activity


Disclosed are dna polymerases having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of rt and polymerase inhibitors relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


07/09/15
20150191707 

Dna polymerase mutants having enhanced template discrimination activity


This invention relates to mutant dna polymerases having an enhanced template discrimination activity compared with the corresponding unmodified dna polymerase counterparts, wherein the amino acid sequence of the mutant dna polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified taq dna polymerase.. .
Integrated Dna Technologies, Inc.


07/02/15
20150184226 

Dna polymerases with improved activity


Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


06/25/15
20150176059 

Oligonucleotide inhibitor of dna polymerases


The invention comprises a reversible oligonucleotide inhibitor of nucleic acid polymerases. Methods of designing said inhibitors and using said inhibitors in amplification and detection of nucleic acid, particularly detection of rna by rt-pcr are also disclosed..
Roche Molecular Systems, Inc.


06/25/15
20150175948 

Nucleic acid amplification reactor


Provided is a nucleic acid amplification reactor that can easily perform a nucleic acid amplification reaction. A nucleic acid amplification reactor 1 includes a reaction chamber 20 to which a thermoplastic hydrogel 50 is applied.

06/18/15
20150167070 

Dual enzymatic amplification


Provided are methods for validating the presence and character of genomic mutations, particularly single nucleotide polymorphisms (snps), by parallel amplification of a portion or the whole genome with at least two different dna polymerases.. .
Cynvenio Biosystems, Inc.


06/11/15
20150159146 

Mutant neq hs dna polymerase derived from nanoarchaeum equitans and its application to hot-start pcr


A dna polymerase (neq dna polymerase) derived from nanoarchaeum equitans is split into neq l and neq s fragments, each of which contains inteins. A neq hot-start (hs) dna polymerase in which the inteins of the neq l and neq s fragments are linked with each other is provided in the form of a precursor of neq dna polymerase.
Research & Business Foundation Sungkyunkwan University


06/11/15
20150157700 

Adenoviral vector-based malaria vaccine


The invention provides an adenovirus or adenoviral vector characterized by comprising a nucleic acid sequence encoding one or more plasmodium antigens and one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein, as well as a method of inducing an immune response against plasmodium falciparum in a mammal by administering the adenovirus or adenoviral vector to the mammal.. .
Ganvec, Inc.


06/04/15
20150152396 

Novel dna polymerases


Novel proteins having dna polymerase are described which have utility in amplification reactions and have improved properties over bst polymerase such as for example enhanced reverse transcriptase activity.. .
New England Biolabs, Inc.


04/30/15
20150118687 

Method and predicting susceptibility to a developmental disorder


A nucleotide sequence signal amplification composition that includes an isolated, synthetic nucleotide sequence of greater than 7 nucleotides. The sequence is a fragment of seq id no:3 and further comprising a t nucleotide at position 1438 of seq id no:3 and one or more primers that bind to the synthetic nucleotide sequence, a thermostable dna polymerase, restriction enzyme, or a combination thereof..
Centre For Addiction And Mental Health


04/16/15
20150104848 

Nucleic acid-free thermostable enzymes and methods of production thereof


The present invention provides thermostable enzymes, such as dna polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (pcr)..
Life Technologies Corporation


03/26/15
20150087557 

Enzyme composition for dna end repair, adenylation, phosphorylation


Enzyme compositions and their method of use that provide ready-to-use master mixtures. The compositions comprise a modified thermophilic dna polymerase lacking 5′-3′ and 3′-5′ exonuclease activity premixed with t4 dna polymerase, klenow fragment and t4 polynucleotide kinase and all other necessary components, including reaction buffer and nucleoside triphosphates, required to perform dna blunting, phosphorylation, and single nucleotide extension reactions in one tube and in two steps.
Thermo Fisher Scientific Baltics Uab


03/19/15
20150079635 

Isothermal amplification using oligocation-conjugated primer sequences


Provided herein are methods and kits for isothermal nucleic acid amplifications that use an oligocation-oligonucleotide conjugate primer for amplifying a target nucleic acid to generate amplicons. Isothermal dna amplification methods that employ a strand displacing dna polymerase and polyamine-oligonucleotide conjugate primer are also provided..
General Electric Company


03/05/15
20150064747 

Modified epstein-barr virus dna polymerase and methods for isothermal dna amplification


Modified epstein barr virus dna polymerase for use in nucleic acid amplification, including isothermal nucleic acid amplification, in vitro are provided. Methods using and kits comprising epstein barr virus dna polymerase and its variants of this invention for nucleic acid amplification in vitro, including isothermal dna amplification, are also provided..
University Of Connecticut


02/19/15
20150051230 

Methods and compositions for inhibition of polymerase


The present invention is directed to methods and compositions for inhibition of viral nucleic acid polymerases, such as rna and dna polymerases, and methods and compositions that are useful for treating viral infections in subjects. The methods comprise administering to the subject a therapeutically effective amount of a compound of formula i, or a pharmaceutically acceptable salt or hydrate thereof, or a composition comprising a compound of formula i, or a pharmaceutically acceptable salt or hydrate thereof, and a pharmaceutically acceptable carrier.
Biocryst Pharmaceuticals, Inc.


02/19/15
20150050660 

Stable compositions for nucleic acid amplification and sequencing


The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable dna polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing.
Life Technologies Corporation


02/19/15
20150050644 

Method for detecting pyrophosphoric acid using support having boronic acid group immobilized thereon


An object of the present invention is to provide a method capable of easily and directly performing dna sequencing by directly detecting pyrophosphoric acid released by an enzymatic reaction, particularly with a dna polymerase or a dna ligase. The present invention provides a method and a device for detecting pyrophosphoric acid by measuring an electrical change occurring when a boronic acid group immobilized on an electrically conductive support reversibly binds to pyrophosphoric acid..
Hitachi, Ltd.


02/12/15
20150044683 

Composition for hot-start reverse transcription reaction or hot-start reverse transcription polymerase chain reaction


A composition for hot-start reverse transcription reaction and a composition for reverse transcription pcr are disclosed. The composition is obtained by adding pyrophosphate and pyrophosphatase to an aqueous solution containing reaction buffer solution, mgcl2, four kinds of dntps, and reverse transcription polymerase in a single reaction tube.
Bioneer Corporation


02/12/15
20150044674 

Hyperthermophilic polymerase enabled proximity extension assay


The present invention relates to a proximity probe based detection assay (“proximity assay”) for an analyte in a sample, specifically a proximity probe extension assay (pea), an in particular to an improvement in the method to reduce non-specific “background” signals, wherein the improvement comprises the use in such assays of a hyperthermophilic polymerase, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) extending the 3′ end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the extension reaction comprises increasing the temperature of assay above room temperature and uses a polymerase enzyme which is characterised as having less than 20% of its maximal enzyme activity at 40° c. And having less than 10% of its maximal enzyme activity at 25° c., wherein the optimum temperature for maximal activity of the polymerase is more than 40° c.
Olink Ab


01/15/15
20150017688 

Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)


The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (rt-pcr). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step rt-pcr procedure using combinations of reverse transcriptase and thermostable dna polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin.
Life Technologies Corporation


01/01/15
20150004617 

Dna polymerase activity assays and methods enabling determination of viable microbes


A method for performing a diagnostic assay for the detection of the presence or amount of a microorganism within a sample matrix containing active dna polymerase, is disclosed. The method utilizes the measurement of dna polymerase extension activity, wherein the assay comprises the steps of incubating dna polymerase in the sample matrix with a selected suitable substrate, and performing pcr cycling and detection via the use of a selected suitable nucleic acid probe, thereby to detect endogenous dna polymerase extension activity in the sample matrix as an indication of the presence or amount of said microorganism..
Zeus Scientific, Inc.


12/25/14
20140378340 

Methods for genotyping


Novel methods and kits are disclosed for reducing the complexity of a nucleic acid sample to interrogate a collection of target sequences, for example, to discriminating between alleles at polymorphic positions in a genome. Complexity reduction can be accomplished by extension of a capture probes followed by amplification of the extended capture probe using common primers.
Affymetrix, Inc.


12/25/14
20140377810 

Ssb-polymerase fusion proteins


Fusion proteins comprising a single strand dna binding protein and a nucleic acid polymerase (e.g. dna polymerase or reverse transcriptase).
Life Technologies Corporation


12/11/14
20140364322 

Isothermal amplification systems and methods


The present invention relates to systems and methods for performing isothermal amplification reactions, in particular, denaturation methods for use in isothermal amplification reactions. An exemplary method may comprise: a) contacting a target nucleic acid with an electrode, wherein the electrode surface has a plurality of first and optionally second nucleic acid primers immobilized thereon, and wherein a target nucleic acid hybridizes to at least one of said first and second nucleic acid primers; b) extending at least one of the first and second primers using a dna polymerase to form extended target nucleic acids; c) applying positive electrical bias to the electrode such that the extended target nucleic acids anneal to one of the first and second primers; d) extending the target nucleic acid with a dna polymerase to form amplified target nucleic acid; e) reversing the electrical bias such that the amplified target nucleic acid is denatured from the surface..

12/11/14
20140363875 

Novel dna polymerase


Provided are: a dna polymerase comprising: an amino acid sequence modified from the amino acid sequence of seq id no: 12, which has a substitution of arginine at position 651 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid; and a dna polymerase comprising an amino acid sequence modified from the amino acid sequence of seq id no: 14, which has a substitution of proline at position 653 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid.. .

12/11/14
20140363849 

Method for improving nucleic acid synthesis reaction


Provided are the following: a method, for improving reactivity of a nucleic acid synthesis reaction, comprising a step for adding an ω-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising dna polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an ω-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ω-amino acid.. .

12/11/14
20140363848 

Chimeric dna polymerases


The present invention provides, among other things, chimeric dna polymerases containing heterologous domains having sequences derived from at least two dna polymerases that have at least one distinct functional characteristics (e.g., elongation rate, processivity, error rate or fidelity, salt tolerance or resistance) and methods of making and using the same. In some embodiments, the present invention can combine desired functional characteristics (e.g., high processivity; high elongation rate; thermostability; resistance to salt, pcr additives (e.g., pcr enhancers) and other impurities; and high fidelity) of different dna polymerases in a chimeric polymerase..

11/27/14
20140349285 

Polymerase driven nesa


The present invention relates to a novel method for detecting a target polynucleotide having a target sequence, comprising (a) exposing the target polynucleotide to an initiating oligonucleotide; (b) extending the initiating oligonucleotide with an extended sequence complementary to the target sequence; (c) ligating the initiating oligonucleotide sequence with the extended sequence to form a circular oligonucleotide having a nicking endonuclease (ne) recognition/cutting sequence; (d) exposing the circular oligonucleotide to a dna polymerase and a dna synthesis primer to synthesize dna having a ne recognition sequence; (e) exposing the synthesized dna to a probe having the ne recognition/cutting sequence to form a double stranded dna having a full ne site; (f) exposing the double stranded dna to a nicking endonuclease (ne) to cleave the probe; and (g) detecting the cleaved probe. The presence of the cleaved probe indicates the presence of the target polynucleotide..

11/20/14
20140342922 

Nucleic acid amplification method


A nucleic acid amplification method includes ligating a double-stranded adapter (20) containing adapter dna strands capable of forming a folded structure to a double-stranded dna (1, 2) containing a target dna sequence (1) to prepare a cyclic dna template composed of double-stranded dna containing a nick (5). A 3′-end elongation reaction is performed using a strand-displacement dna polymerase from the nick (5) as an origin, thereby producing a concatemer (29) in which a plurality of the target dna sequences (1) and the adapter dna strands capable of forming the folded structure are linked in series as a single-stranded dna.

11/20/14
20140342409 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

11/20/14
20140342365 

Method of determining the nucleotide sequence of oligonucleotides and dna molecules


The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection.

11/13/14
20140335509 

Method of determining the nucleotide sequence of oligonucleotides and dna molecules


The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection.

10/30/14
20140322793 

Novel dna polymerases


A745 is an amino acid residue having a non-polar aliphatic side chain).. .

10/30/14
20140322761 

Method of preparing sample for nucleic acid amplification reaction, nucleic acid amplification method, and reagent and microchip for solid phase nucleic acid amplification reaction


Provided is a method of preparing a sample for nucleic acid amplification reaction, including: a procedure of dissolving a solid phase reagent at least containing dna polymerase, cyclodextrin, and a binder, in a liquid containing a nucleic acid.. .

10/30/14
20140322759 

Phi29 dna polymerase mutants having increased thermostability and processivity


Mutants of bacteriophage phi29 dna polymerase with increased protein stability and increased half-life, compared to wild type dna polymerase. The disclosed mutants are more stable in reaction mixtures with or without dna.

10/23/14
20140315211 

Methods for suppression pcr


Provided herein are approaches for the detection, identification, and/or selective amplification of specific target species or target variants of nucleic acid sequences, even within an excess of unwanted similar sequences or variants. These approaches include methods, assays, and kits for suppression pcr that require, in part, dna polymerase that lacks 5′-3′ exonuclease activity, and a pcr primer, termed a forward selective primer or a nunchaku primer.

10/23/14
20140314717 

Simian (gorilla) adenovirus or adenoviral vectors and methods of use


The invention provides an adenovirus or adenoviral vector characterized by comprising one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein.. .

10/16/14
20140308710 

Peg-mediated assembly of nucleic acid molecules


The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a dna polymerase, a mixture of dntps, and a crowding agent to form an assembly mixture.

10/16/14
20140308672 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

10/09/14
20140302508 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

10/09/14
20140302507 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

10/02/14
20140296082 

Dna polymerase variants with reduced exonuclease activity and uses thereof


Compositions and methods are described to modify family b dna polymerases that contain residual exonuclease activity that interferes with sequencing techniques and with detection of single nucleotide polymorphisms. The compositions are mutant proteins with reduced exonuclease activity compared with presently available “exo−” polymerases, and a sensitive screening assay that enables an assessment of exonuclease activity of any synthetic dna polymerase..

10/02/14
20140295500 

Dna polymerases with improved activity


Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.

10/02/14
20140295499 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

09/25/14
20140287415 

Polymerases


Modified dna polymerases have an affinity for dna such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate dna templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-dna complexes in each reaction cycle.

09/18/14
20140271711 

Adenoviral vector-based respiratory syncytial virus (rsv) vaccine


The invention provides an adenovirus or adenoviral vector characterized by comprising a nucleic acid sequence encoding one or more respiratory syncytial virus (rsv) antigens and one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein, as well as a method of inducing an immune response against rsv in a mammal by administering the adenovirus or adenoviral vector to the mammal.. .

09/04/14
20140248308 

Affenadenovirus (gorilla) or adenoviral vectors and methods of use


The invention provides an adenovirus or adenoviral vector characterized by comprising one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein.. .

09/04/14
20140248307 

Affenadenovirus (gorilla) or adenoviral vectors and methods of use


The invention provides an adenovirus or adenoviral vector characterized by comprising one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein.. .

08/21/14
20140234940 

Mutant rb69 dna polymerase


Provided herein are mutant dna-dependent polymerases which are derived from, or otherwise related to, wild type rb69 dna polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides.

08/21/14
20140234909 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

08/21/14
20140234846 

Recombinase polymerase amplification


This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes.

08/14/14
20140227743 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

08/14/14
20140227696 

Method and predicting susceptibility to a developmental disorder


A nucleotide sequence signal amplification composition that includes an isolated, synthetic nucleotide sequence of greater than 7 nucleotides. The sequence is a fragment of seq id no:3 and further comprising a t nucleotide at position 1438 of seq id no:3 and one or more primers that bind to the synthetic nucleotide sequence, a thermostable dna polymerase, a restriction enzyme, or a combination thereof..

08/07/14
20140220639 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

07/31/14
20140212929 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

07/31/14
20140212883 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.



Dna Polymerase topics: Dna Polymerase, Polymerase, Nucleic Acid, Nucleotide, Recombinant, Amplification, Sequencing, Exonuclease, Reverse Transcriptase, Dna Molecule, Isothermal, Amino Acid, Oligonucleotide, Endonuclease, Polynucleotide

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