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This page is updated frequently with new Dna Polymerase-related patent applications. Subscribe to the Dna Polymerase RSS feed to automatically get the update: related Dna RSS feeds. RSS updates for this page: Dna Polymerase RSS RSS


Dna polymerases and related methods

Pacific Biosciences Of California

Recombinant polymerases with increased phototolerance


Date/App# patent app List of recent Dna Polymerase-related patents
08/20/15
20150232822 
 Dna polymerases having improved labeled nucleotide incorporation properties patent thumbnailDna polymerases having improved labeled nucleotide incorporation properties
In addition to providing novel mutant dna polymerases, the invention also provides polynucleotides encoding the subject mutant dna polymerases. The polynucleotides provided may comprise expression vectors for the recombinant production of the mutant polymerases.

08/20/15
20150232821 
 Thermostable type-a dna polymerase mutant with increased resistance to inhibitors in blood patent thumbnailThermostable type-a dna polymerase mutant with increased resistance to inhibitors in blood
The invention provides mutants of dna polymerases having an enhanced resistance to inhibitors of dna polymerase activity. The mutant polymerases are well suited for pcr amplification of targets in samples that contain inhibitors of wild-type polymerases..
Agilent Technologies, Inc.


08/06/15
20150218537 
 Dna polymerases and related methods patent thumbnailDna polymerases and related methods
Disclosed are mutant dna polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


08/06/15
20150218535 
 Recombinant polymerases with increased phototolerance patent thumbnailRecombinant polymerases with increased phototolerance
Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like.
Pacific Biosciences Of California


08/06/15
20150218534 
 Modified dna polymerase patent thumbnailModified dna polymerase
The modified dna polymerase comprises modifications of at least two amino acids selected from the group consisting of amino acids corresponding to y7, p36, and v93 in seq id no: 1, in the amino acid sequence represented by any one of seq id nos: 1 to 10.. .

07/09/15
20150191781 
 Random-primed transcriptase in-vitro transcription  rna amplification patent thumbnailRandom-primed transcriptase in-vitro transcription rna amplification
A random-primed reverse transcriptase-in vitro transcription method of linearly amplifying rna is provided. According to the methods of the invention, source rna (or other single-stranded nucleic acid), preferably, mrna, is converted to double-stranded cdna using two random primers, one of which comprises a rna polymerase promoter sequence (“promoter-primer”), to yield a double-stranded cdna that comprises a rna polymerase promoter that is recognized by a rna polymerase.
Life Technologies Corporation


07/09/15
20150191708 
 Dna polymerases with improved activity patent thumbnailDna polymerases with improved activity
Disclosed are dna polymerases having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of rt and polymerase inhibitors relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


07/09/15
20150191707 
 Dna polymerase mutants having enhanced template discrimination activity patent thumbnailDna polymerase mutants having enhanced template discrimination activity
This invention relates to mutant dna polymerases having an enhanced template discrimination activity compared with the corresponding unmodified dna polymerase counterparts, wherein the amino acid sequence of the mutant dna polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified taq dna polymerase.. .
Integrated Dna Technologies, Inc.


07/02/15
20150184226 
 Dna polymerases with improved activity patent thumbnailDna polymerases with improved activity
Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


06/25/15
20150176059 
 Oligonucleotide inhibitor of dna polymerases patent thumbnailOligonucleotide inhibitor of dna polymerases
The invention comprises a reversible oligonucleotide inhibitor of nucleic acid polymerases. Methods of designing said inhibitors and using said inhibitors in amplification and detection of nucleic acid, particularly detection of rna by rt-pcr are also disclosed..
Roche Molecular Systems, Inc.


06/25/15
20150175948 

Nucleic acid amplification reactor


Provided is a nucleic acid amplification reactor that can easily perform a nucleic acid amplification reaction. A nucleic acid amplification reactor 1 includes a reaction chamber 20 to which a thermoplastic hydrogel 50 is applied.

06/18/15
20150167070 

Dual enzymatic amplification


Provided are methods for validating the presence and character of genomic mutations, particularly single nucleotide polymorphisms (snps), by parallel amplification of a portion or the whole genome with at least two different dna polymerases.. .
Cynvenio Biosystems, Inc.


06/11/15
20150159146 

Mutant neq hs dna polymerase derived from nanoarchaeum equitans and its application to hot-start pcr


A dna polymerase (neq dna polymerase) derived from nanoarchaeum equitans is split into neq l and neq s fragments, each of which contains inteins. A neq hot-start (hs) dna polymerase in which the inteins of the neq l and neq s fragments are linked with each other is provided in the form of a precursor of neq dna polymerase.
Research & Business Foundation Sungkyunkwan University


06/11/15
20150157700 

Adenoviral vector-based malaria vaccine


The invention provides an adenovirus or adenoviral vector characterized by comprising a nucleic acid sequence encoding one or more plasmodium antigens and one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein, as well as a method of inducing an immune response against plasmodium falciparum in a mammal by administering the adenovirus or adenoviral vector to the mammal.. .
Ganvec, Inc.


06/04/15
20150152396 

Novel dna polymerases


Novel proteins having dna polymerase are described which have utility in amplification reactions and have improved properties over bst polymerase such as for example enhanced reverse transcriptase activity.. .
New England Biolabs, Inc.


04/30/15
20150118687 

Method and predicting susceptibility to a developmental disorder


A nucleotide sequence signal amplification composition that includes an isolated, synthetic nucleotide sequence of greater than 7 nucleotides. The sequence is a fragment of seq id no:3 and further comprising a t nucleotide at position 1438 of seq id no:3 and one or more primers that bind to the synthetic nucleotide sequence, a thermostable dna polymerase, restriction enzyme, or a combination thereof..
Centre For Addiction And Mental Health


04/16/15
20150104848 

Nucleic acid-free thermostable enzymes and methods of production thereof


The present invention provides thermostable enzymes, such as dna polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (pcr)..
Life Technologies Corporation


03/26/15
20150087557 

Enzyme composition for dna end repair, adenylation, phosphorylation


Enzyme compositions and their method of use that provide ready-to-use master mixtures. The compositions comprise a modified thermophilic dna polymerase lacking 5′-3′ and 3′-5′ exonuclease activity premixed with t4 dna polymerase, klenow fragment and t4 polynucleotide kinase and all other necessary components, including reaction buffer and nucleoside triphosphates, required to perform dna blunting, phosphorylation, and single nucleotide extension reactions in one tube and in two steps.
Thermo Fisher Scientific Baltics Uab


03/19/15
20150079635 

Isothermal amplification using oligocation-conjugated primer sequences


Provided herein are methods and kits for isothermal nucleic acid amplifications that use an oligocation-oligonucleotide conjugate primer for amplifying a target nucleic acid to generate amplicons. Isothermal dna amplification methods that employ a strand displacing dna polymerase and polyamine-oligonucleotide conjugate primer are also provided..
General Electric Company


03/05/15
20150064747 

Modified epstein-barr virus dna polymerase and methods for isothermal dna amplification


Modified epstein barr virus dna polymerase for use in nucleic acid amplification, including isothermal nucleic acid amplification, in vitro are provided. Methods using and kits comprising epstein barr virus dna polymerase and its variants of this invention for nucleic acid amplification in vitro, including isothermal dna amplification, are also provided..
University Of Connecticut


02/19/15
20150051230 

Methods and compositions for inhibition of polymerase


The present invention is directed to methods and compositions for inhibition of viral nucleic acid polymerases, such as rna and dna polymerases, and methods and compositions that are useful for treating viral infections in subjects. The methods comprise administering to the subject a therapeutically effective amount of a compound of formula i, or a pharmaceutically acceptable salt or hydrate thereof, or a composition comprising a compound of formula i, or a pharmaceutically acceptable salt or hydrate thereof, and a pharmaceutically acceptable carrier.
Biocryst Pharmaceuticals, Inc.


02/19/15
20150050660 

Stable compositions for nucleic acid amplification and sequencing


The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable dna polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing.
Life Technologies Corporation


02/19/15
20150050644 

Method for detecting pyrophosphoric acid using support having boronic acid group immobilized thereon


An object of the present invention is to provide a method capable of easily and directly performing dna sequencing by directly detecting pyrophosphoric acid released by an enzymatic reaction, particularly with a dna polymerase or a dna ligase. The present invention provides a method and a device for detecting pyrophosphoric acid by measuring an electrical change occurring when a boronic acid group immobilized on an electrically conductive support reversibly binds to pyrophosphoric acid..
Hitachi, Ltd.


02/12/15
20150044683 

Composition for hot-start reverse transcription reaction or hot-start reverse transcription polymerase chain reaction


A composition for hot-start reverse transcription reaction and a composition for reverse transcription pcr are disclosed. The composition is obtained by adding pyrophosphate and pyrophosphatase to an aqueous solution containing reaction buffer solution, mgcl2, four kinds of dntps, and reverse transcription polymerase in a single reaction tube.
Bioneer Corporation


02/12/15
20150044674 

Hyperthermophilic polymerase enabled proximity extension assay


The present invention relates to a proximity probe based detection assay (“proximity assay”) for an analyte in a sample, specifically a proximity probe extension assay (pea), an in particular to an improvement in the method to reduce non-specific “background” signals, wherein the improvement comprises the use in such assays of a hyperthermophilic polymerase, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) extending the 3′ end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the extension reaction comprises increasing the temperature of assay above room temperature and uses a polymerase enzyme which is characterised as having less than 20% of its maximal enzyme activity at 40° c. And having less than 10% of its maximal enzyme activity at 25° c., wherein the optimum temperature for maximal activity of the polymerase is more than 40° c.
Olink Ab


01/15/15
20150017688 

Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)


The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (rt-pcr). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step rt-pcr procedure using combinations of reverse transcriptase and thermostable dna polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin.
Life Technologies Corporation


01/01/15
20150004617 

Dna polymerase activity assays and methods enabling determination of viable microbes


A method for performing a diagnostic assay for the detection of the presence or amount of a microorganism within a sample matrix containing active dna polymerase, is disclosed. The method utilizes the measurement of dna polymerase extension activity, wherein the assay comprises the steps of incubating dna polymerase in the sample matrix with a selected suitable substrate, and performing pcr cycling and detection via the use of a selected suitable nucleic acid probe, thereby to detect endogenous dna polymerase extension activity in the sample matrix as an indication of the presence or amount of said microorganism..
Zeus Scientific, Inc.


12/25/14
20140378340 

Methods for genotyping


Novel methods and kits are disclosed for reducing the complexity of a nucleic acid sample to interrogate a collection of target sequences, for example, to discriminating between alleles at polymorphic positions in a genome. Complexity reduction can be accomplished by extension of a capture probes followed by amplification of the extended capture probe using common primers.
Affymetrix, Inc.


12/25/14
20140377810 

Ssb-polymerase fusion proteins


Fusion proteins comprising a single strand dna binding protein and a nucleic acid polymerase (e.g. dna polymerase or reverse transcriptase).
Life Technologies Corporation


12/11/14
20140364322 

Isothermal amplification systems and methods


The present invention relates to systems and methods for performing isothermal amplification reactions, in particular, denaturation methods for use in isothermal amplification reactions. An exemplary method may comprise: a) contacting a target nucleic acid with an electrode, wherein the electrode surface has a plurality of first and optionally second nucleic acid primers immobilized thereon, and wherein a target nucleic acid hybridizes to at least one of said first and second nucleic acid primers; b) extending at least one of the first and second primers using a dna polymerase to form extended target nucleic acids; c) applying positive electrical bias to the electrode such that the extended target nucleic acids anneal to one of the first and second primers; d) extending the target nucleic acid with a dna polymerase to form amplified target nucleic acid; e) reversing the electrical bias such that the amplified target nucleic acid is denatured from the surface..

12/11/14
20140363875 

Novel dna polymerase


Provided are: a dna polymerase comprising: an amino acid sequence modified from the amino acid sequence of seq id no: 12, which has a substitution of arginine at position 651 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid; and a dna polymerase comprising an amino acid sequence modified from the amino acid sequence of seq id no: 14, which has a substitution of proline at position 653 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid.. .

12/11/14
20140363849 

Method for improving nucleic acid synthesis reaction


Provided are the following: a method, for improving reactivity of a nucleic acid synthesis reaction, comprising a step for adding an ω-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising dna polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an ω-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ω-amino acid.. .

12/11/14
20140363848 

Chimeric dna polymerases


The present invention provides, among other things, chimeric dna polymerases containing heterologous domains having sequences derived from at least two dna polymerases that have at least one distinct functional characteristics (e.g., elongation rate, processivity, error rate or fidelity, salt tolerance or resistance) and methods of making and using the same. In some embodiments, the present invention can combine desired functional characteristics (e.g., high processivity; high elongation rate; thermostability; resistance to salt, pcr additives (e.g., pcr enhancers) and other impurities; and high fidelity) of different dna polymerases in a chimeric polymerase..

11/27/14
20140349285 

Polymerase driven nesa


The present invention relates to a novel method for detecting a target polynucleotide having a target sequence, comprising (a) exposing the target polynucleotide to an initiating oligonucleotide; (b) extending the initiating oligonucleotide with an extended sequence complementary to the target sequence; (c) ligating the initiating oligonucleotide sequence with the extended sequence to form a circular oligonucleotide having a nicking endonuclease (ne) recognition/cutting sequence; (d) exposing the circular oligonucleotide to a dna polymerase and a dna synthesis primer to synthesize dna having a ne recognition sequence; (e) exposing the synthesized dna to a probe having the ne recognition/cutting sequence to form a double stranded dna having a full ne site; (f) exposing the double stranded dna to a nicking endonuclease (ne) to cleave the probe; and (g) detecting the cleaved probe. The presence of the cleaved probe indicates the presence of the target polynucleotide..

11/20/14
20140342922 

Nucleic acid amplification method


A nucleic acid amplification method includes ligating a double-stranded adapter (20) containing adapter dna strands capable of forming a folded structure to a double-stranded dna (1, 2) containing a target dna sequence (1) to prepare a cyclic dna template composed of double-stranded dna containing a nick (5). A 3′-end elongation reaction is performed using a strand-displacement dna polymerase from the nick (5) as an origin, thereby producing a concatemer (29) in which a plurality of the target dna sequences (1) and the adapter dna strands capable of forming the folded structure are linked in series as a single-stranded dna.

11/20/14
20140342409 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

11/20/14
20140342365 

Method of determining the nucleotide sequence of oligonucleotides and dna molecules


The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection.

11/13/14
20140335509 

Method of determining the nucleotide sequence of oligonucleotides and dna molecules


The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection.

10/30/14
20140322793 

Novel dna polymerases


A745 is an amino acid residue having a non-polar aliphatic side chain).. .

10/30/14
20140322761 

Method of preparing sample for nucleic acid amplification reaction, nucleic acid amplification method, and reagent and microchip for solid phase nucleic acid amplification reaction


Provided is a method of preparing a sample for nucleic acid amplification reaction, including: a procedure of dissolving a solid phase reagent at least containing dna polymerase, cyclodextrin, and a binder, in a liquid containing a nucleic acid.. .

10/30/14
20140322759 

Phi29 dna polymerase mutants having increased thermostability and processivity


Mutants of bacteriophage phi29 dna polymerase with increased protein stability and increased half-life, compared to wild type dna polymerase. The disclosed mutants are more stable in reaction mixtures with or without dna.

10/23/14
20140315211 

Methods for suppression pcr


Provided herein are approaches for the detection, identification, and/or selective amplification of specific target species or target variants of nucleic acid sequences, even within an excess of unwanted similar sequences or variants. These approaches include methods, assays, and kits for suppression pcr that require, in part, dna polymerase that lacks 5′-3′ exonuclease activity, and a pcr primer, termed a forward selective primer or a nunchaku primer.

10/23/14
20140314717 

Simian (gorilla) adenovirus or adenoviral vectors and methods of use


The invention provides an adenovirus or adenoviral vector characterized by comprising one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein.. .

10/16/14
20140308710 

Peg-mediated assembly of nucleic acid molecules


The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a dna polymerase, a mixture of dntps, and a crowding agent to form an assembly mixture.

10/16/14
20140308672 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

10/09/14
20140302508 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

10/09/14
20140302507 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

10/02/14
20140296082 

Dna polymerase variants with reduced exonuclease activity and uses thereof


Compositions and methods are described to modify family b dna polymerases that contain residual exonuclease activity that interferes with sequencing techniques and with detection of single nucleotide polymorphisms. The compositions are mutant proteins with reduced exonuclease activity compared with presently available “exo−” polymerases, and a sensitive screening assay that enables an assessment of exonuclease activity of any synthetic dna polymerase..

10/02/14
20140295500 

Dna polymerases with improved activity


Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.

10/02/14
20140295499 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

09/25/14
20140287415 

Polymerases


Modified dna polymerases have an affinity for dna such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate dna templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-dna complexes in each reaction cycle.

09/18/14
20140271711 

Adenoviral vector-based respiratory syncytial virus (rsv) vaccine


The invention provides an adenovirus or adenoviral vector characterized by comprising a nucleic acid sequence encoding one or more respiratory syncytial virus (rsv) antigens and one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein, as well as a method of inducing an immune response against rsv in a mammal by administering the adenovirus or adenoviral vector to the mammal.. .

09/04/14
20140248308 

Affenadenovirus (gorilla) or adenoviral vectors and methods of use


The invention provides an adenovirus or adenoviral vector characterized by comprising one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein.. .

09/04/14
20140248307 

Affenadenovirus (gorilla) or adenoviral vectors and methods of use


The invention provides an adenovirus or adenoviral vector characterized by comprising one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein.. .

08/21/14
20140234940 

Mutant rb69 dna polymerase


Provided herein are mutant dna-dependent polymerases which are derived from, or otherwise related to, wild type rb69 dna polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides.

08/21/14
20140234909 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

08/21/14
20140234846 

Recombinase polymerase amplification


This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes.

08/14/14
20140227743 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

08/14/14
20140227696 

Method and predicting susceptibility to a developmental disorder


A nucleotide sequence signal amplification composition that includes an isolated, synthetic nucleotide sequence of greater than 7 nucleotides. The sequence is a fragment of seq id no:3 and further comprising a t nucleotide at position 1438 of seq id no:3 and one or more primers that bind to the synthetic nucleotide sequence, a thermostable dna polymerase, a restriction enzyme, or a combination thereof..

08/07/14
20140220639 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

07/31/14
20140212929 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

07/31/14
20140212883 

Dna polymerases with increased 3'-mismatch discrimination


Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.

07/24/14
20140206567 

Method for detecting target nucleic acid


The disclosure of the present description provides a method for detecting a target nucleic acid, whereby probe hybridization can be accomplished efficiently. To that end, a target nucleic acid is amplified using a first primer having a tag sequence complementary to a detection probe pre-associated with the target nucleic acid and a first recognition sequence that recognizes a first base sequence in the target nucleic acid and also having a linking site capable of inhibiting or arresting a dna polymerase reaction disposed between the tag sequence and the first recognition sequence, and a second primer having a second recognition sequence that recognizes a second base sequence in the target nucleic acid, the amplified fragment is brought into contact with a detection probe so as to allow hybridization, and the hybridization product is detected..

07/24/14
20140206001 

Methods and compositions for enrichment of nucleic acids in mixtures of highly homologous sequences


Provided are methods of enrichment and detection of target nucleic acids during target amplification in the presence of excess amounts of highly homologous sequences, said methods having substantial diagnostic utility (e.g., cancer diagnostics). Provided are amplification reaction mixtures having at least one cleavage-directing oligonucleotide, the respective binding sites of which, for the target and homologous sequences, include one or more nucleotide positions differing in sequence between the target homologous sequences.

07/17/14
20140199699 

Compositions and methods for rt-pcr


The present invention relates to methods and compositions having trehalose and dna polymerase for facilitating the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of rna molecules, and for increasing the detection sensitivity and reliability through generation of secure cdna molecules prior to gene-specific primer dependent amplification. The reagent mixture comprises a ready to use reagent solution, wherein the solution comprises: (a) trehalose in a concentration between about 5% and about 35%; (b) a viral reverse transcriptase; and (c) at least one dna polymerases, in a buffer suitable for use in a reverse transcription reaction, wherein the buffer comprises a co-factor metal ion and nucleoside triphosphates..

07/03/14
20140186894 

Isolated dna polymerases, kits and applications thereof


Isolated dna polymerase and the mutant dna polymerases thereof are provided. The dna polymerases have good thermostability..

06/26/14
20140178911 

Characterization of thermostable dna polymerase


Methods detecting covalent lysine modifications in dna polymerases are provided. These methods are particularly useful in determining the extent and location of a lysine modification in a dna polymerase..

06/19/14
20140170730 

Dna polymerases with improved activity


Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.

06/19/14
20140170707 

Method and amplification of nucleic acid sequences by using thermal convection


The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using dna polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region..

06/12/14
20140162897 

Transposon end compositions and methods for modifying nucleic acids


The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target dna in vitro, then using a dna polymerase for generating 5′- and 3′-tagged single-stranded dna fragments without performing a pcr amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged dna fragments for use in a variety of processes, including processes for metagenomic analysis of dna in environmental samples, copy number variation (cnv) analysis of dna, and comparative genomic sequencing (cgs), including massively parallel dna sequencing (so-called “next-generation sequencing.).

06/05/14
20140154748 

Thermostable chimeric nucleic acid polymerases and uses thereof


Novel thermostable chimeric nucleic acid polymerases and methods for their generation and use are disclosed. It is shown that these chimeric nucleic acid polymerases, such as dna polymerases, can be constructed using enzymatically active domains, isolated from different proteins or chemically synthesized.

05/22/14
20140141434 

Recombinase polymerase amplification


This disclosure describe three related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of the bacterial reca and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods has the advantage of not requiring thermocycling or thermophilic enzymes.

05/22/14
20140141422 

Fast pcr for str genotyping


Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (str) using a family a dna polymerase in a fast pcr protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time pcr methods using the two-step protocol and kits for str profiling using the fast pcr protocol..

05/15/14
20140134633 

Detergent free polymerases


The present invention relates to a formulation of a thermostable dna polymerase which is completely free of detergents and its particular use in real time polymerase chain reaction (pcr). Such a formulation may be obtained if the selected purification method does not require the addition of a detergent at any purification step..

05/08/14
20140127694 

Dna polymerase


The present invention relates to dna polymerases. In particular the invention relates to a method for the generation of dna polymerases exhibiting a relaxed substrate specificity.

04/24/14
20140115741 

F. oxysporum f.sp. melonis race 1,2-resistant melons


Methods for conveying fusarium oxysporum f.sp. Melonis (fom) race 1,2 resistance into non-resistant melon germplasm are provided.

04/24/14
20140113291 

Salt-tolerant dna polymerases


Four novel sequences of type b dna polymerases and variants and analogues thereof useful for applications involving dna polymerization in high salt conditions.. .

04/10/14
20140100238 

Method of diagnosing and treating epstein barr virus-based myalgic encephalomyelitis chronic fatigue syndrome patients


A method of diagnosing a subset of epstein barr virus, myalgic encephalomyelitis chronic fatigue syndrome (me/cfs) patients through a multi-prong clinical/serological analysis is provided wherein epstein barr virus abortive lytic replication (ebv) is determined as the specific causal agent through the use of serum antibodies to ebv encoded dutpase and serum antibodies to ebv dna polymerase as molecular markers. A method of treating patients diagnosed with epstein barr virus abortive lytic replication (ebv), myalgic encephalomyelitis chronic fatigue syndrome (me/cfs) with specific antiviral nucleosides is also provided, to alleviate the condition..

04/10/14
20140099674 

Recombinase polymerase amplification


This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.

04/03/14
20140094375 

Recombinant polymerases for incorporation of protein shield nucleotide analogs


Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing or nucleic acid amplification. Such properties include enhanced performance with large nucleotide analogs, increased stability, increased readlength, and improved detection of modified bases, and can also include resistance to photodamage, enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased accuracy, altered speed, increased cosolvent resistance, and the like.

04/03/14
20140094374 

Recombinant polymerases with increased readlength and stability for single-molecule sequencing


Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing or nucleic acid amplification. Such properties include increased stability, increased readlength, enhanced performance with large nucleotide analogs, and improved detection of modified bases, and can also include resistance to photodamage, enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased accuracy, altered speed, increased cosolvent resistance, and the like.

04/03/14
20140093938 

Method of removing nucleic acid contamination in reverse transcription and amplification reactions


The invention provides methods of removing nucleic acid contamination from reverse transcription reactions and hot-start pcr, wherein said hot-start pcr is a barrier hot-start pcr set up and/or involves a hot-start dna polymerase, which methods comprise use of a dnase that is substantially irreversibly inactivated by heating at a temperature of about 50° c. For 5 minutes, and that is substantially specific for double stranded dna.

04/03/14
20140093878 

Mutant endonuclease v enzymes and applications thereof


Provided herein are mutant endonuclease v enzymes that are capable of nicking an inosine-containing dna sequence. Nucleic acid assays and agents that employ such mutant endonuclease v enzymes to introduce a nick into a target dna including one or more inosine, and uses a dna polymerase to generate amplicons of a target dna are also described..

03/27/14
20140087382 

Normalization of polymerase activity


Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of dna polymerases, such as taq dna polymerase.. .

02/27/14
20140057268 

Detection of an amplification reaction product using ph-sensitive dyes


Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or pcr amplification reactions to completion using ph-sensitive dyes that are either colored or fluorescent.

02/20/14
20140051590 

Compositions and methods for detection of herpes simplex virus 1 and 2


Methods for the rapid detection of the presence or absence of herpes simplex virus 1 and 2 (hsv-1 and hsv-2) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step.

02/20/14
20140051126 

Dna polymerases with improved activity


Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.

01/30/14
20140030765 

Modified dna polymerases for improved amplification


The present invention provides improved dna polymerases that may be better suited for applications in recombinant dna technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified dna polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications..

01/23/14
20140026251 

Soy gene cluster regions and methods of use


Methods for conveying pathogen resistance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing pathogen resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with pathogen resistance.

01/16/14
20140017671 

Detection nucleic acid and kit and using thereof


A detection method of nucleic acid is provided. The method includes: providing nucleic acid to be tested, making the nucleic acid to be tested react under asymmetric pcr conditions with a pair of primers for target nucleic acid amplification, dna polymerase, adenine deoxyribonucleotide, guanine deoxyribonucleotide, cytosine deoxyribonucleotide and thymidine deoxyribonucleotide in a pcr buffer solution, mixing the reaction product and liquid that contains probe molecules, and judging whether the nucleic acid to be tested contains the target nucleic acid by observing the obtained mixture color or color change.

01/02/14
20140005147 

Dna polymerase inhibitors composition and methods


A topical composition containing a dna polymerase inhibitor for removing hair as well as methods of inducing hair loss is described.. .

01/02/14
20140004509 

Kit for isothermal dna amplification starting from an rna template


A kit for amplifying deoxynucleic acid (dna) from ribonucleic acid (rna) template is provided. The kit for amplifying a rna comprises at least one inosine-containing primer; and at least one enzyme comprising a reverse transcriptase activity, a strand displacement dna polymerase activity, a nuclease acitivity for nicking dna 3′ to an inosine residue of the primer or combinations thereof.

01/02/14
20140004508 

Method for isothermal dna amplification starting from an rna template


A method of amplifying rna template is provided. The method comprises reverse-transcribing a ribonucleic acid (rna) template to form a cdna using a first reaction mixture comprising rna template, at least one primer capable of hybridizing to the rna template, a reverse transcriptase and deoxynucleoside triphosphates (dntps); and amplifying the cdna to form an amplified product using a second reaction mixture comprising at least one strand displacement dna polymerase, at least one inosine-containing primer and a nuclease that is capable of nicking dna 3′ to an inosine residue of the primer.



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Dna Polymerase topics: Dna Polymerase, Polymerase, Nucleic Acid, Nucleotide, Recombinant, Amplification, Sequencing, Exonuclease, Reverse Transcriptase, Dna Molecule, Isothermal, Amino Acid, Oligonucleotide, Endonuclease, Polynucleotide

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