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Dna Polymerase

Dna Polymerase-related patent applications - as published by the U.S. Patent and Trademark Office (USPTO).


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NEW In vitro recombination method
Synthetic Genomics, Inc.
August 17, 2017 - N°20170233764

The present invention relates, e. G., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) dna molecules of interest, wherein the distal region of the first dna molecule and the proximal region of the second dna molecule share a region of sequence identity, comprising contacting the two dna molecules in a reaction mixture with (a) ...
Simian (gorilla) adenovirus or adenoviral vectors and methods of use
Genvec, Inc.
July 27, 2017 - N°20170211097

The invention provides an adenovirus or adenoviral vector characterized by comprising one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein.
Antibodies that bind thermophilic dna polymerases
Thermo Fisher Scientific Baltics Uab
July 20, 2017 - N°20170204454

This disclosure relates to antibodies that bind thermophilic family b dna polymerases. Related uses, methods, and compositions are also provided.
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Nras gene mutation detection kit
Jiaxing Accb Diagnostics Ltd.
July 13, 2017 - N°20170198355

This invention relates to a kit for detecting nras gene mutation, and this kit can be used to detect cancer-related nras gene mutation. The said kit comprises: (1) the internal reference detection reagent, which includes the internal reference gene specific primers, internal reference gene specific probes and dntp solution; (2) the nras mutation detection reagent, which includes the nras gene mutant type ...
Dna polymerases with improved activity
Roche Molecular Systems, Inc.
July 06, 2017 - N°20170191044

Disclosed are dna polymerases having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of rt and polymerase inhibitors relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e. G., for production of ...
Pore-forming protein conjugate compositions and methods
The Trustees Of Columbia University In The City Of New York
June 22, 2017 - N°20170175183

The present disclosure relates to compositions and methods based on a fast, efficient chemical reaction for conjugating a pore-forming protein, such as α-hemolysin, to a biomolecule, such as antibodies, receptors, and enzymes, such as dna polymerase, and the use of such pore-forming protein conjugates in nanopore devices and methods.
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Dimeric reverse transcriptase
Bio-rad Laboratories, Inc.
June 08, 2017 - N°20170159032

Covalently-linked dna polymerases are provided.
Fusion polymerase and method for using the same
New England Biolabs, Inc.
June 01, 2017 - N°20170152494

This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a dna polymerase; and (b) a heterologous sequence-specific dna binding domain. A method for copying a dna template, as well as a kit for performing the same, are also described.
Fusion polymerase and method for using the same
New England Biolabs, Inc.
June 01, 2017 - N°20170152493

This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a dna polymerase; and (b) a heterologous sequence-specific dna binding domain. A method for copying a dna template, as well as a kit for performing the same, are also described.
Method of diagnosing and treating epstein barr virus-based myalgic encephalomyelitis chronic fatigue syndrome patients
New England Biolabs, Inc.
May 25, 2017 - N°20170146534

A method of diagnosing a subset of epstein barr virus, myalgic encephalomyelitis chronic fatigue syndrome (me/cfs) patients through a multi-prong clinical/serological analysis is provided wherein epstein barr virus abortive lytic replication (ebv) is determined as the specific causal agent through the use of serum antibodies to ebv encoded dutpase and serum antibodies to ebv dna polymerase as molecular ...
Modified nucleotide reagents
New England Biolabs, Inc.
May 25, 2017 - N°20170145502

Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with ...
Labeled nucleotide analogs, reaction mixtures, and methods and systems for sequencing
New England Biolabs, Inc.
May 25, 2017 - N°20170145496

Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with ...
Protected dye-labeled reagents
New England Biolabs, Inc.
May 25, 2017 - N°20170145495

Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with ...
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Isothermal amplification under low salt condition
General Electric Company
May 04, 2017 - N°20170121747

Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a dna polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dntp) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. ...
Dna sequencing detection field effect transistor
International Business Machines Corporation
April 27, 2017 - N°20170114402

A semiconductor structure is provided that can be used for dna sequencing detection. The semiconductor structure includes a doped epitaxial source semiconductor material structure located on a first portion of a semiconductor substrate and a doped epitaxial drain semiconductor material structure located on a second portion of the semiconductor substrate. A gate dielectric portion is located on a third portion ...
Dna polymerase mutants having enhanced template discrimination activity
Integrated Dna Technologies, Inc.
April 27, 2017 - N°20170114379

This invention relates to mutant dna polymerases having an enhanced template discrimination activity compared with the corresponding unmodified dna polymerase counterparts, wherein the amino acid sequence of the mutant dna polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified taq dna polymerase.
Dna polymerases with improved activity
Roche Molecular Systems, Inc.
April 13, 2017 - N°20170101632

Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e. G., for production of the dna polymerases.
Fast pcr for str genotyping
Life Technologies Corporation
March 23, 2017 - N°20170081715

Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (str) using a ...
Method and kit of parts for extraction of nucleic acids
Ifp Privates Institut Fur Produktqualitat Gmbh
March 23, 2017 - N°20170081704

Composition, method and kit of parts for a simplified and safe extraction of nucleic acids from biological samples, such as crude and processed food, and subsequent analysis by polymerase chain reaction to the presence of animal material, genetically modified organisms, allergens and pathogens. The method comprises the addition of extracting composition together with hot water for extraction and stabilization of ...
Dna polymerases
Thermo Fisher Scientific Baltics Uab
March 23, 2017 - N°20170081646

A dna polymerase mutant comprising a taq dna polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: e189k, e230k, e507k, h28r, l30r, g38r, f73v, h75r, e76a, e76g, e76k, e90k, k206r, e315k, a348v, l351f, a439t, ...
Method and reagent for enrichment of circulating tumor dna
Haplox Biotechnology(shenzhen) Co., Ltd.
March 09, 2017 - N°20170067117

The present invention provides a method and a reagent for enrichment of circulating tumor dna, the method comprising the steps of mixing a water phase and an oil phase and shaking the mixture to prepare an emulsion pcr reaction system, and performing emulsion pcr amplification, wherein the water phase comprises peripheral blood plasma dna as template dna, a forward primer ...
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