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Dna Polymerase

Dna Polymerase-related patent applications - as published by the U.S. Patent and Trademark Office (USPTO).


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Compositions, methods, and kits for synthesis and detection of nucleic acids
Life Technologies Corporation
October 12, 2017 - N°20170292144

Compositions, methods, and kits for synthesizing, detecting, and/or quantifying nucleic acids are provided herein. Embodiments comprise a nucleic acid amplification composition comprising a thermostable dna polymerase and agents which improve nucleic acid synthesis, amplification, detection, and/or quantification of nucleic acid targets in a crude extract or crude lysate sample.
Compositions and methods relating to synthetic rna polynucleotides created from synthetic dna oligonucleotides
New England Biolabs, Inc.
October 05, 2017 - N°20170283797

Compositions and methods are provided for forming a single rna polynucleotide from a plurality of dna oligonucleotides in a single reaction chamber using combined reagents in a single step reaction. Dna polymerase, rna polymerase and single stranded (ss) dna oligonucleotides are combined where each dna oligonucleotide has one or more sequence modules, wherein one sequence module in the first ss ...
Polymerases
Illumina Cambridge Limited
September 28, 2017 - N°20170275602

Modified dna polymerases have an affinity for dna such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate dna templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-dna complexes in each reaction cycle. The modified polymerases may be used in a number of dna ...
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Integrated on chip detector and zero waveguide module structure for use in dna sequencing
International Business Machines Corporation
September 14, 2017 - N°20170263801

A semiconductor structure for use in single molecule real time dna sequencing technology is provided. The structure includes a semiconductor substrate including a first region and an adjoining second region. A photodetector is present in the first region and a plurality of semiconductor devices is present in the second region. A contact wire is located on a surface of a ...
Method for improving nucleic acid synthesis reaction
Takara Bio Inc.
September 14, 2017 - N°20170260578

Provided are the following: a method, for improving reactivity of an acid synthesis reaction, comprising a step for adding an ω-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising dna polymerase, reaction buffer, at least one primer, at least one deoxyribonucleoside triphosphate, and an ω-amino acid; and a reaction buffer, ...
Affenadenovirus (gorilla) or adenoviral vectors and methods of use
Genvec, Inc.
August 31, 2017 - N°20170246318

The invention provides an adenovirus or adenoviral vector characterized by comprising one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein.
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In vitro recombination method
Synthetic Genomics, Inc.
August 17, 2017 - N°20170233764

The present invention relates, e. G., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) dna molecules of interest, wherein the distal region of the first dna molecule and the proximal region of the second dna molecule share a region of sequence identity, comprising contacting the two dna molecules in a reaction mixture with (a) ...
Simian (gorilla) adenovirus or adenoviral vectors and methods of use
Genvec, Inc.
July 27, 2017 - N°20170211097

The invention provides an adenovirus or adenoviral vector characterized by comprising one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein.
Antibodies that bind thermophilic dna polymerases
Thermo Fisher Scientific Baltics Uab
July 20, 2017 - N°20170204454

This disclosure relates to antibodies that bind thermophilic family b dna polymerases. Related uses, methods, and compositions are also provided.
Thermophilic dna polymerase mutants
Thermo Fisher Scientific Baltics Uab
July 20, 2017 - N°20170204384

This disclosure relates to thermophilic family b dna polymerases comprising a neutral amino acid residue at a certain position near the c-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3′-5′ ...
Nras gene mutation detection kit
Jiaxing Accb Diagnostics Ltd.
July 13, 2017 - N°20170198355

This invention relates to a kit for detecting nras gene mutation, and this kit can be used to detect cancer-related nras gene mutation. The said kit comprises: (1) the internal reference detection reagent, which includes the internal reference gene specific primers, internal reference gene specific probes and dntp solution; (2) the nras mutation detection reagent, which includes the nras gene mutant type ...
Dna polymerases with improved activity
Roche Molecular Systems, Inc.
July 06, 2017 - N°20170191044

Disclosed are dna polymerases having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of rt and polymerase inhibitors relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e. G., for production of ...
Pore-forming protein conjugate compositions and methods
The Trustees Of Columbia University In The City Of New York
June 22, 2017 - N°20170175183

The present disclosure relates to compositions and methods based on a fast, efficient chemical reaction for conjugating a pore-forming protein, such as α-hemolysin, to a biomolecule, such as antibodies, receptors, and enzymes, such as dna polymerase, and the use of such pore-forming protein conjugates in nanopore devices and methods.
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Dimeric reverse transcriptase
Bio-rad Laboratories, Inc.
June 08, 2017 - N°20170159032

Covalently-linked dna polymerases are provided.
Fusion polymerase and method for using the same
New England Biolabs, Inc.
June 01, 2017 - N°20170152494

This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a dna polymerase; and (b) a heterologous sequence-specific dna binding domain. A method for copying a dna template, as well as a kit for performing the same, are also described.
Fusion polymerase and method for using the same
New England Biolabs, Inc.
June 01, 2017 - N°20170152493

This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a dna polymerase; and (b) a heterologous sequence-specific dna binding domain. A method for copying a dna template, as well as a kit for performing the same, are also described.
Method of diagnosing and treating epstein barr virus-based myalgic encephalomyelitis chronic fatigue syndrome patients
New England Biolabs, Inc.
May 25, 2017 - N°20170146534

A method of diagnosing a subset of epstein barr virus, myalgic encephalomyelitis chronic fatigue syndrome (me/cfs) patients through a multi-prong clinical/serological analysis is provided wherein epstein barr virus abortive lytic replication (ebv) is determined as the specific causal agent through the use of serum antibodies to ebv encoded dutpase and serum antibodies to ebv dna polymerase as molecular ...
Modified nucleotide reagents
New England Biolabs, Inc.
May 25, 2017 - N°20170145502

Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with ...
Labeled nucleotide analogs, reaction mixtures, and methods and systems for sequencing
New England Biolabs, Inc.
May 25, 2017 - N°20170145496

Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with ...
Protected dye-labeled reagents
New England Biolabs, Inc.
May 25, 2017 - N°20170145495

Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with ...
Solid phase isothermal amplification
General Electric Company
May 18, 2017 - N°20170137874

Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a dna polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dntp) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. ...
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