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Dna Polymerase patents



      
           
This page is updated frequently with new Dna Polymerase-related patent applications. Subscribe to the Dna Polymerase RSS feed to automatically get the update: related Dna RSS feeds. RSS updates for this page: Dna Polymerase RSS RSS


Date/App# patent app List of recent Dna Polymerase-related patents
08/21/14
20140234940
 Mutant rb69 dna polymerase patent thumbnailMutant rb69 dna polymerase
Provided herein are mutant dna-dependent polymerases which are derived from, or otherwise related to, wild type rb69 dna polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides.
08/21/14
20140234909
 Dna polymerases with increased 3'-mismatch discrimination patent thumbnailDna polymerases with increased 3'-mismatch discrimination
Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
08/21/14
20140234846
 Recombinase polymerase amplification patent thumbnailRecombinase polymerase amplification
This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes.
08/14/14
20140227743
 Dna polymerases with increased 3'-mismatch discrimination patent thumbnailDna polymerases with increased 3'-mismatch discrimination
Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
08/14/14
20140227696
 Method and apparatus for predicting susceptibility to a developmental disorder patent thumbnailMethod and apparatus for predicting susceptibility to a developmental disorder
A nucleotide sequence signal amplification composition that includes an isolated, synthetic nucleotide sequence of greater than 7 nucleotides. The sequence is a fragment of seq id no:3 and further comprising a t nucleotide at position 1438 of seq id no:3 and one or more primers that bind to the synthetic nucleotide sequence, a thermostable dna polymerase, a restriction enzyme, or a combination thereof..
08/07/14
20140220639
 Dna polymerases with increased 3'-mismatch discrimination patent thumbnailDna polymerases with increased 3'-mismatch discrimination
Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
07/31/14
20140212929
 Dna polymerases with increased 3'-mismatch discrimination patent thumbnailDna polymerases with increased 3'-mismatch discrimination
Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
07/31/14
20140212883
 Dna polymerases with increased 3'-mismatch discrimination patent thumbnailDna polymerases with increased 3'-mismatch discrimination
Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
07/24/14
20140206567
 Method for detecting target nucleic acid patent thumbnailMethod for detecting target nucleic acid
The disclosure of the present description provides a method for detecting a target nucleic acid, whereby probe hybridization can be accomplished efficiently. To that end, a target nucleic acid is amplified using a first primer having a tag sequence complementary to a detection probe pre-associated with the target nucleic acid and a first recognition sequence that recognizes a first base sequence in the target nucleic acid and also having a linking site capable of inhibiting or arresting a dna polymerase reaction disposed between the tag sequence and the first recognition sequence, and a second primer having a second recognition sequence that recognizes a second base sequence in the target nucleic acid, the amplified fragment is brought into contact with a detection probe so as to allow hybridization, and the hybridization product is detected..
07/24/14
20140206001
 Methods and compositions for enrichment of nucleic acids in mixtures of highly homologous sequences patent thumbnailMethods and compositions for enrichment of nucleic acids in mixtures of highly homologous sequences
Provided are methods of enrichment and detection of target nucleic acids during target amplification in the presence of excess amounts of highly homologous sequences, said methods having substantial diagnostic utility (e.g., cancer diagnostics). Provided are amplification reaction mixtures having at least one cleavage-directing oligonucleotide, the respective binding sites of which, for the target and homologous sequences, include one or more nucleotide positions differing in sequence between the target homologous sequences.
07/17/14
20140199699
Compositions and methods for rt-pcr
The present invention relates to methods and compositions having trehalose and dna polymerase for facilitating the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of rna molecules, and for increasing the detection sensitivity and reliability through generation of secure cdna molecules prior to gene-specific primer dependent amplification. The reagent mixture comprises a ready to use reagent solution, wherein the solution comprises: (a) trehalose in a concentration between about 5% and about 35%; (b) a viral reverse transcriptase; and (c) at least one dna polymerases, in a buffer suitable for use in a reverse transcription reaction, wherein the buffer comprises a co-factor metal ion and nucleoside triphosphates..
07/03/14
20140186894
Isolated dna polymerases, kits and applications thereof
Isolated dna polymerase and the mutant dna polymerases thereof are provided. The dna polymerases have good thermostability..
06/26/14
20140178911
Characterization of thermostable dna polymerase
Methods detecting covalent lysine modifications in dna polymerases are provided. These methods are particularly useful in determining the extent and location of a lysine modification in a dna polymerase..
06/19/14
20140170730
Dna polymerases with improved activity
Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
06/19/14
20140170707
Method and apparatus for amplification of nucleic acid sequences by using thermal convection
The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using dna polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region..
06/12/14
20140162897
Transposon end compositions and methods for modifying nucleic acids
The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target dna in vitro, then using a dna polymerase for generating 5′- and 3′-tagged single-stranded dna fragments without performing a pcr amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged dna fragments for use in a variety of processes, including processes for metagenomic analysis of dna in environmental samples, copy number variation (cnv) analysis of dna, and comparative genomic sequencing (cgs), including massively parallel dna sequencing (so-called “next-generation sequencing.).
06/05/14
20140154748
Thermostable chimeric nucleic acid polymerases and uses thereof
Novel thermostable chimeric nucleic acid polymerases and methods for their generation and use are disclosed. It is shown that these chimeric nucleic acid polymerases, such as dna polymerases, can be constructed using enzymatically active domains, isolated from different proteins or chemically synthesized.
05/22/14
20140141434
Recombinase polymerase amplification
This disclosure describe three related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of the bacterial reca and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods has the advantage of not requiring thermocycling or thermophilic enzymes.
05/22/14
20140141422
Fast pcr for str genotyping
Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (str) using a family a dna polymerase in a fast pcr protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time pcr methods using the two-step protocol and kits for str profiling using the fast pcr protocol..
05/15/14
20140134633
Detergent free polymerases
The present invention relates to a formulation of a thermostable dna polymerase which is completely free of detergents and its particular use in real time polymerase chain reaction (pcr). Such a formulation may be obtained if the selected purification method does not require the addition of a detergent at any purification step..
05/08/14
20140127694
Dna polymerase
The present invention relates to dna polymerases. In particular the invention relates to a method for the generation of dna polymerases exhibiting a relaxed substrate specificity.
04/24/14
20140115741
F. oxysporum f.sp. melonis race 1,2-resistant melons
Methods for conveying fusarium oxysporum f.sp. Melonis (fom) race 1,2 resistance into non-resistant melon germplasm are provided.
04/24/14
20140113291
Salt-tolerant dna polymerases
Four novel sequences of type b dna polymerases and variants and analogues thereof useful for applications involving dna polymerization in high salt conditions.. .
04/10/14
20140100238
Method of diagnosing and treating epstein barr virus-based myalgic encephalomyelitis chronic fatigue syndrome patients
A method of diagnosing a subset of epstein barr virus, myalgic encephalomyelitis chronic fatigue syndrome (me/cfs) patients through a multi-prong clinical/serological analysis is provided wherein epstein barr virus abortive lytic replication (ebv) is determined as the specific causal agent through the use of serum antibodies to ebv encoded dutpase and serum antibodies to ebv dna polymerase as molecular markers. A method of treating patients diagnosed with epstein barr virus abortive lytic replication (ebv), myalgic encephalomyelitis chronic fatigue syndrome (me/cfs) with specific antiviral nucleosides is also provided, to alleviate the condition..
04/10/14
20140099674
Recombinase polymerase amplification
This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.
04/03/14
20140094375
Recombinant polymerases for incorporation of protein shield nucleotide analogs
Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing or nucleic acid amplification. Such properties include enhanced performance with large nucleotide analogs, increased stability, increased readlength, and improved detection of modified bases, and can also include resistance to photodamage, enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased accuracy, altered speed, increased cosolvent resistance, and the like.
04/03/14
20140094374
Recombinant polymerases with increased readlength and stability for single-molecule sequencing
Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing or nucleic acid amplification. Such properties include increased stability, increased readlength, enhanced performance with large nucleotide analogs, and improved detection of modified bases, and can also include resistance to photodamage, enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased accuracy, altered speed, increased cosolvent resistance, and the like.
04/03/14
20140093938
Method of removing nucleic acid contamination in reverse transcription and amplification reactions
The invention provides methods of removing nucleic acid contamination from reverse transcription reactions and hot-start pcr, wherein said hot-start pcr is a barrier hot-start pcr set up and/or involves a hot-start dna polymerase, which methods comprise use of a dnase that is substantially irreversibly inactivated by heating at a temperature of about 50° c. For 5 minutes, and that is substantially specific for double stranded dna.
04/03/14
20140093878
Mutant endonuclease v enzymes and applications thereof
Provided herein are mutant endonuclease v enzymes that are capable of nicking an inosine-containing dna sequence. Nucleic acid assays and agents that employ such mutant endonuclease v enzymes to introduce a nick into a target dna including one or more inosine, and uses a dna polymerase to generate amplicons of a target dna are also described..
03/27/14
20140087382
Normalization of polymerase activity
Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of dna polymerases, such as taq dna polymerase.. .
02/27/14
20140057268
Detection of an amplification reaction product using ph-sensitive dyes
Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or pcr amplification reactions to completion using ph-sensitive dyes that are either colored or fluorescent.
02/20/14
20140051590
Compositions and methods for detection of herpes simplex virus 1 and 2
Methods for the rapid detection of the presence or absence of herpes simplex virus 1 and 2 (hsv-1 and hsv-2) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step.
02/20/14
20140051126
Dna polymerases with improved activity
Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
01/30/14
20140030765
Modified dna polymerases for improved amplification
The present invention provides improved dna polymerases that may be better suited for applications in recombinant dna technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified dna polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications..
01/23/14
20140026251
Soy gene cluster regions and methods of use
Methods for conveying pathogen resistance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing pathogen resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with pathogen resistance.
01/16/14
20140017671
Detection method of nucleic acid and kit and using thereof
A detection method of nucleic acid is provided. The method includes: providing nucleic acid to be tested, making the nucleic acid to be tested react under asymmetric pcr conditions with a pair of primers for target nucleic acid amplification, dna polymerase, adenine deoxyribonucleotide, guanine deoxyribonucleotide, cytosine deoxyribonucleotide and thymidine deoxyribonucleotide in a pcr buffer solution, mixing the reaction product and liquid that contains probe molecules, and judging whether the nucleic acid to be tested contains the target nucleic acid by observing the obtained mixture color or color change.
01/02/14
20140005147
Dna polymerase inhibitors composition and methods
A topical composition containing a dna polymerase inhibitor for removing hair as well as methods of inducing hair loss is described.. .
01/02/14
20140004509
Kit for isothermal dna amplification starting from an rna template
A kit for amplifying deoxynucleic acid (dna) from ribonucleic acid (rna) template is provided. The kit for amplifying a rna comprises at least one inosine-containing primer; and at least one enzyme comprising a reverse transcriptase activity, a strand displacement dna polymerase activity, a nuclease acitivity for nicking dna 3′ to an inosine residue of the primer or combinations thereof.
01/02/14
20140004508
Method for isothermal dna amplification starting from an rna template
A method of amplifying rna template is provided. The method comprises reverse-transcribing a ribonucleic acid (rna) template to form a cdna using a first reaction mixture comprising rna template, at least one primer capable of hybridizing to the rna template, a reverse transcriptase and deoxynucleoside triphosphates (dntps); and amplifying the cdna to form an amplified product using a second reaction mixture comprising at least one strand displacement dna polymerase, at least one inosine-containing primer and a nuclease that is capable of nicking dna 3′ to an inosine residue of the primer.
12/26/13
20130344491
Random-primed transcriptase in-vitro transcription method for rna amplification
A random-primed reverse transcriptase-in vitro transcription method of linearly amplifying rna is provided. According to the methods of the invention, source rna (or other single-stranded nucleic acid), preferably, mrna, is converted to double-stranded cdna using two random primers, one of which comprises a rna polymerase promoter sequence (“promoter-primer”), to yield a double-stranded cdna that comprises a rna polymerase promoter that is recognized by a rna polymerase.
12/19/13
20130338117
Therapeutic compounds for blocking dna synthesis of pox viruses
This invention provides methods of inhibiting replication of a poxvirus by contacting a poxvirus with a compound having formula i, formula xxi, formula xxxii, or formula xli which in turn reduce, inhibit, or abrogate poxvirus dna polymerase activity and/or its interaction with its processivity factor. Formula i, formula xxi, formula xxxii, or formula xli can be utilized to treat humans and animals suffering from a poxvirus infection.
12/12/13
20130331404
Methods and compositions for inhibition of polymerase
The present invention is directed to methods and compositions for inhibition of viral nucleic acid polymerases, such as rna and dna polymerases, and methods and compositions that are useful for treating viral infections in subjects. The methods comprise administering to the subject a therapeutically effective amount of a compound of formula i, or a pharmaceutically acceptable salt or hydrate thereof, or a composition comprising a compound of formula i, or a pharmaceutically acceptable salt or hydrate thereof, and a pharmaceutically acceptable carrier.
12/12/13
20130330734
Controlled inhibition and re-activation of dna polymerases by cleavable oligonucleotide inhibitors
A hot start enzyme composition is described that includes a hot start nuclease, a nucleic acid polymerase, and a substantially double-stranded oligonucleotide that inhibits the catalytic activity of the nucleic acid polymerase at temperatures lower than the melting temperature of the oligonucleotide.. .
12/05/13
20130323795
Endonuclase-assisted isothermal amplification using contamination-free reagents
Disclosed are methods and kits for endonuclease-assisted dna amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease v enzymes to introduce a nick into a target dna comprising at least one inosine, and a dna polymerase to generate amplicons of a target dna are also disclosed..
11/21/13
20130309684
Nucleic acid-free thermostable enzymes and methods of production thereof
The present invention provides thermostable enzymes, such as dna polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (pcr)..
11/07/13
20130295560
Method of determining the nucleotide sequence of oligonucleotides and dna molecules
The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection.
10/31/13
20130288235
Method of determining the nucleotide sequence of oligonucleotides and dna molecules
The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection.
10/17/13
20130273531
Ambient temperature stable kits for molecular diagnostics
A method for processing dna polymerase and/or dntps for use in an amplification procedure, includes providing a solution mixture, the solution mixture including a dna polymerase and/or dntps, a buffer solution and at least one stabilizing agent and hydration reducing the solution mixture. The solution mixture is hydration reduced at a temperature between 0° c.
10/17/13
20130273526
Dna polymerases having improved labeled nucleotide incorporation properties
The invention also provides polynucleotides encoding the mutant dna polymerases, optionally including expression vectors for mutant polymerase recombinant production, and further optionally including host cells containing the polynucleotides. Numerous methods of using the subject dna polymerases are provided, including uses for chain termination sequencing and pcr.
10/03/13
20130260422
Compositions and methods relating to variant dna polymerases and synthetic dna polymerases
Compositions of novel polymerase variants and methods of identifying, making and using these novel polymerases are described. The variants have been shown to have advantageous properties such as increased thermostability, deoxyuridine nucleoside triphosphate tolerance, salt tolerance, reaction speed and/or increased reverse transcriptase properties.
09/26/13
20130252827
Detection of target nucleic acid sequences using dual-labeled immobilized probes on solid phase
The present invention relates to a novel method for detection of target nucleic acid sequences on a solid phase using dual-labeled immobilized probes and its resistance to a 5′ to 3′ exonuclease activity of a dna polymerase. Because the label is remained on the solid substrate by resistance to nucleases due to labeling of a base component the internal nucleotide, the present invention requires no consideration of a suitability of position of the label for remaining on the solid substrate.
09/12/13
20130236886
Phage phi 29 dna polymerase chimera
A dna polymerase chimera comprising an amino-terminal (n-terminal) region encoding a φ29 type dna polymerase and a carboxyl-terminal (c-terminal) region comprising at least one hhh domain which are bound by a connecting amino acid sequence is disclosed along with and the use thereof for replicating, amplifying or sequencing a template dna. Also disclosed is a method for replicating, amplifying or sequencing a deoxyribonucleic acid with a dna polymerase chimera and kits for carrying out the methods..
08/29/13
20130225451
Materials and methods for the synthesis of error-minimized nucleic acid molecules
The present invention provides materials and methods useful for error correction of nucleic acid molecules. In one embodiment of the invention, a first plurality of double-stranded nucleic acid molecules having a nucleotide mismatch are fragmented by exposure to a molecule having unidirectional mismatch endonuclease activity.
08/29/13
20130225419
Quantitative total definition of biologically active sequence elements and positions
A library includes h unique nucleotide sequences involving every position along i continuous positions in a molecule. A method to prepare the library includes obtaining a microarray with a bound probe of up to j nucleotides, j=i+l, for h different probes.
08/22/13
20130217007
Recombinant polymerases with increased phototolerance
Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like.
08/15/13
20130210019
Helicase dependent isothermal amplification using nicking enzymes
The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (hda) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the hda reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a dna polymerase..
08/08/13
20130203122
Reduced inhibition of one-step rt-pcr
The present invention provides a method for amplifying a nucleic acid molecule. The method involves mixing an rna template with a composition having a reverse transcriptase, a dna polymerase and a rt inhibition reducer.
08/01/13
20130196382
Dna polymerases with improved activity
Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
08/01/13
20130196327
Dna polymerase variants with reduced exonuclease activity and uses thereof
Compositions and methods are described to modify family b dna polymerases that contain residual exonuclease activity that interferes with sequencing techniques and with detection of single nucleotide polymorphisms. The compositions are mutant proteins with reduced exonuclease activity compared with presently available “exo−” polymerases, and a sensitive screening assay that enables an assessment of exonuclease activity of any synthetic dna polymerase..
08/01/13
20130196318
Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
The present invention relates generally to the field of detection of microorganisms, in particular detection of bacteria, to methods for measuring enzyme activity, such as dna polymerase activity, and particularly relates to such methods performed on microbial crude lysates, useful for determining microbial enzyme activities which can be linked to amplification signal generators such as real-time polymerase chain reaction (pcr) techniques, thereby enabling determination of microbial pathogens in samples such as unpurified blood and other body fluids. This invention also relates to reagents for use in such methods, and to test kits comprising such reagents for carrying out the methods..
07/25/13
20130189758
Recombinant reverse transcriptases
The present invention relates to a gene that encodes a hyperactive reverse transcriptase having dna polymerase activity and substantially reduced rnase h activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cdna from mrna using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase..
07/11/13
20130177915
Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative pcr
There is provided a method for detecting a target rna molecule in a sample. The method comprises reverse transcribing the target rna contained in the sample using an rt oligonucleotide, the rt oligonucleotide comprising a stem-loop portion containing one or more nucleotides modified or modifiable to block dna polymerase extension and a target annealing portion that is complementary to a downstream portion of the target rna, the target annealing portion located 3′ to the stem-loop portion, to produce a reverse transcription product that comprises the rt oligonucleotide and a 3′ extended region; amplifying the reverse transcription product using (i) a first amplification primer that anneals to a downstream portion of the 3′ extended region of the reverse transcription product and (ii) a second amplification primer that anneals to an interface portion of a dna strand complementary to the reverse transcription product, the interface portion comprising a region that is complementary to a 3′ portion of the rt oligonucleotide and a 5′ portion of the 3′ extended region in the reverse transcription product, to produce an amplification product; and detecting the amplification product; wherein the stem-loop portion adopts a stem-loop structure under conditions used for said reverse transcribing but does not adopt the stem-loop structure under conditions used for said amplifying and wherein when the stem-loop portion contains one or more nucleotides that are modifiable to block dna polymerase extension, the method further comprises modifying the modifiable nucleotide prior to said amplifying..
07/04/13
20130171630
Methods of using telomeres as markers for aging
The invention relates to a simple, reproducible, fast, and accurate method of quantifying and measuring telomeres in a clinical sample. The invention further relates to kits comprising premixed and optimized buffers, dna polymerase, primers, and instructions for the detection of telomere length and quantities.
06/27/13
20130164817
Polypeptides having nucleic acid binding activity
Polynucleotides having nucleic acid binding activity are provided. Methods of stabilizing a nucleic acid duplex are provided.
06/27/13
20130164788
Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)
The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (rt-pcr). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step rt-pcr procedure using combinations of reverse transcriptase and thermostable dna polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin.
06/13/13
20130149749
Polymerase
An engineered dna polymerase characterised in that the polymerase exhibits an enhanced ability to process nucleic acid in the presence of environmental and biological inhibitors compared to wild type dna polymerase.. .
06/13/13
20130149748
Dna polymerases with improved activity
Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
06/13/13
20130149747
Dna polymerases with improved activity
Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
06/13/13
20130149698
Stable compositions for nucleic acid amplification and sequencing
The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable dna polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing.


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Dna Polymerase topics: Dna Polymerase, Polymerase, Nucleic Acid, Nucleotide, Recombinant, Amplification, Sequencing, Exonuclease, Reverse Transcriptase, Dna Molecule, Isothermal, Amino Acid, Oligonucleotide, Endonuclease, Polynucleotide

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