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Dna Polymerase patents

      

This page is updated frequently with new Dna Polymerase-related patent applications.




 Recombinant polymerases for incorporation of protein shield nucleotide analogs patent thumbnailnew patent Recombinant polymerases for incorporation of protein shield nucleotide analogs
Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing or nucleic acid amplification. Such properties include enhanced performance with large nucleotide analogs, increased stability, increased readlength, and improved detection of modified bases, and can also include resistance to photodamage, enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased accuracy, altered speed, increased cosolvent resistance, and the like.
Pacific Biosciences Of California, Inc.


 Method for testing a mutant gene through real time polymerase chain reaction using inhibition of 5'-flap endonuclease activity patent thumbnailMethod for testing a mutant gene through real time polymerase chain reaction using inhibition of 5'-flap endonuclease activity
The present invention relates to a method for detecting single nucleotide polymorphism (snp) using a feature that the 5′-flap endonuclease (fen) activity of dna polymerase is inhibited when a probe complementarily binds to the end of a polymerase chain reaction (pcr) product. More specifically, the present invention relates to a novel method wherein it was verified that, when a probe used for a real-time pcr complementarily binds to the end site of a pcr product, the 5′-fen activity of thermostable dna polymerase to the probe is inhibited, and thus when such a feature is used to make a design such that an snp site to be detected is located at the 5′-end site of the probe, the 5′-flap formation is induced according to the allele, thereby allowing effective snp detection..
Genotech Corp.


 Dna polymerases with increased 3'-mismatch discrimination patent thumbnailDna polymerases with increased 3'-mismatch discrimination
Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.


 Mutant rb69 dna polymerase patent thumbnailMutant rb69 dna polymerase
Provided herein are mutant dna-dependent polymerases which are derived from, or otherwise related to, wild type rb69 dna polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides.
Life Technologies Corporation


 Methods for genotyping patent thumbnailMethods for genotyping
The present invention provides for methods for discriminating between alleles at polymorphic positions in a genome. In general the methods employ allele specific extension of oligonucleotides that are complementary to one of the alleles at the 3′ end of the oligonucleotide.
Affymetrix, Inc.


 High-throughput sequencing detection  methylated cpg islands patent thumbnailHigh-throughput sequencing detection methylated cpg islands
A high-throughput sequencing method for detecting methylated cpg islands includes: processing a dna sample by using a modifier, and converting cytosine in the dna sample into uracil, and keeping 5′methylcytosine unchanged; amplifying the obtained segment by using a primer a and dna polymerase, to obtain a segment having one end being capable of anchoring a junction primer c; amplifying the obtained segment by using a primer b and dna polymerase, to obtain a segment gathering methylated cpg islands and having two ends being capable of separately anchoring junction primers c and d; amplifying the obtained segment at a pcr exponent by using the junction primers c and d and the dna polymerase, to obtain the amplified product; and separating and purifying the amplified product, to form a high-throughput sequencing library and perform computer sequencing, and data analysis.. .
Peking University


 Mutated dna polymerases with high selectivity and activity patent thumbnailMutated dna polymerases with high selectivity and activity
The present invention provides dna polymerases and their use in various applications. The present invention also relates to uses and methods utilizing the dna polymerase of the present invention for discriminating between matched and mismatched primers or detecting snp or methylated cytosine.
Universitaet Konstanz


 Novel compounds useful for the treatment of bacterial infectious diseases patent thumbnailNovel compounds useful for the treatment of bacterial infectious diseases
Novel compounds of the invention may be prepared as a pharmaceutical composition, and may be useful in the treatment of infectious diseases, in particular bacterial infectious diseases. The compounds may be active against a specific enzyme in the bacterial dna replicative process, dna polymerase iiie..

 Methods and compositions for repair of dna ends by multiple enzymatic activities patent thumbnailMethods and compositions for repair of dna ends by multiple enzymatic activities
Provided herein are compositions for and methods of generating ligation-competent nucleic acids. In some aspects, the compositions comprise exonuclease iii, t4 dna polymerase, klenow, and/or t4 polynucleotide kinase..
Rubicon Genomics, Inc.


 Isothermal amplification using oligocation-conjugated primer sequences patent thumbnailIsothermal amplification using oligocation-conjugated primer sequences
Provided herein are methods and kits for isothermal nucleic acid amplifications that use an oligocation-oligonucleotide conjugate primer for amplifying a target nucleic acid to generate amplicons. Isothermal dna amplification methods that employ a strand displacing dna polymerase and polyamine-oligonucleotide conjugate primer are also provided..
General Electric Company


Recombinase polymerase amplification

This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.
Alere San Diego Inc.

Methods and compositions for interference with dna polymerase and dna synthesis

Disclosed are methods and compositions for inhibiting dna synthesis in a cell using rna. Inhibition of dna synthesis by rna can be used, for example, in analytical methods, as a research tool to affect cells under study, to synchronize cell cycle in a cell culture, and to inhibit cell growth.
Georgia State University Research Foundation, Inc.

Mutant dna polymerase blends and mutant dna polymerases

A thermostable dna polymerase composition comprising at least two dna polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, dna sequencing, nucleic acid amplification and cdna synthesis..
Life Technologies Corporation

Enzyme preparation containing thermostable dna polymerase, producing same, and detecting subject organism to be detected

Disclosed is a thermostable dna polymerase preparation which can illimitably reduce the risk of false positivity in the detection of a subject microorganism utilizing a gene amplification reaction and therefore enables the selective amplification of dna for detecting the subject microorganism even when the amount of the subject microorganism is small and therefore the amount of dna collected therefrom is extremely small, and can be produced at a reduced cost. Also disclosed is a method for quantifying or quantifying/identifying a subject organism to be detected rapidly, conveniently and with high sensitivity using the preparation of the present invention..
National University Corporation University Of Toyama

Recombinant polymerases with increased phototolerance

Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like.
Pacific Biosciences Of California, Inc.

Methods and compositions for rapid seamless dna assembly

Provided herein are methods for assembling dna fragments employing at least three enzymatic activities: dna polymerase, flap endonuclease, and dna ligase. Certain aspects include methods for generating closed circular dna products, e.g., plasmid vectors, by assembling various dna fragments having complementary ends that hybridize to one another.
Agilent Technologies, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Modified polymerase compositions, methods and kits

This invention relates to type i dna polymerases, for example, taq dna polymerase, and to methods and reagent kits utilizing such polymerases.. .
Brandeis University

Integrated on chip detector and zero waveguide module structure for use in dna sequencing

A semiconductor structure for use in single molecule real time dna sequencing technology is provided. The structure includes a semiconductor substrate including a first region and an adjoining second region.
International Business Machines Corporation

Nucleic acid amplification method using allele-specific reactive primer

The detection method using the allele-specific reactive primer (asrp) according to the present invention is a technique having a very high specificity of amplification due to the characteristics of the asrp and a proofreading dna polymerase. The detection method can effectively detect mutations (point mutation, insertion, deletion, etc.) including a single nucleotide polymorphism (snp), and can also be used to detect methylation of cpg after bisulfite treatment or to amplify and detect a target dna starting with a desired nucleotide sequence from a dna library..

Ph measurement for sequencing of dna

The present method involves sequencing by synthesis in which a template strand having an attached primer is immobilized in a small volume reaction mixture. In one embodiment, the reaction mixture is in contact with a sensitive heat sensor, which detects the heat of reaction from incorporation of a complementary base (dntp) in the presence of appropriate reagents (dna polymerase, and polymerase reaction buffer).

A the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides

Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during dna polymerase-mediated replication of dna or rna polymerase-mediated transcription of rna. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded dna or rna), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker..
Synthorx, Inc.

Mutant endonuclease v enzymes and applications thereof

Provided herein are mutant endonuclease v enzymes that are capable of nicking an inosine-containing dna sequence. Nucleic acid assays and agents that employ such mutant endonuclease v enzymes to introduce a nick into a target dna including one or more inosine, and uses a dna polymerase to generate amplicons of a target dna are also described..
General Electric Company

Recombinant reverse transcriptases

The present invention relates to a gene that encodes a hyperactive reverse transcriptase having dna polymerase activity and substantially reduced rnase h activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cdna from mrna using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase..
Applied Biosystems, Llc

Fusion polymerase and using the same

This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a dna polymerase; and (b) a heterologous sequence-specific dna binding domain. A method for copying a dna template, as well as a kit for performing the same, are also described..
New England Biolabs, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Design, synthesis and use of synthetic nucleotides comprising charge mass tags

Embodiments of the present disclosure relate generally to reporter compositions which are synthetic nucleotides that comprise nucleotides with a high charge mass moiety attached thereto via a linker molecule. The linker molecules can vary in length in part to enable the high charge mass moiety to extend out from a dna polymerase complex so that polymerization may not be influenced..
Quantumdx Group Limited

Method of determining the nucleotide sequence of oligonucleotides and dna molecules

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection.
Life Technologies Corporation

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Gene expression analysis

The present invention is directed to methods and kits for gene analysis. The methods of the invention comprise the steps of providing nucleic acid; synthesis of a single-stranded dna that is complementary to said nucleic acid molecule by contacting the nucleic acid with a dna polymerase, a primer and a mixture of dntps under conditions that allow the generation of the dna, wherein the primer comprises a target-complementary region and wherein the dntp mixture comprises datp, dgtp, dctp, dttp and dutp; cleaving the dna 5′ to du sites by (i) contacting the dna with an uracil deglycosylase to generate a basic sites at positions of dutp incorporation in the dna; and (ii) contacting the dna with an apurinic/apyrimidinic (ap) endonuclease; contacting the dna comprising at its 5′-end the nucleotide sequence of the primer with a ssdna ligase to circularize the dna; and sequencing the circularized cdna.
Rheinische Friedrich-wilhelms-universität Bonn

Affenadenovirus (gorilla) or adenoviral vectors and methods of use

The invention provides an adenovirus or adenoviral vector characterized by comprising one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pix protein, dna polymerase protein, penton protein, hexon protein, and/or fiber protein.. .
Genvec, Inc.

Polypeptides having nucleic acid binding activity

Polynucleotides having nucleic acid binding activity are provided. Methods of stabilizing a nucleic acid duplex are provided.
Applied Biosystems, Llc

Polymerases

Modified dna polymerases have an affinity for dna such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate dna templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-dna complexes in each reaction cycle.
Illumina Cambridge Limited

Mutant dna polymerases and methods of use

The present invention provides mutant dna polymerases, polynucleotides encoding the polymerases, cassettes and vectors including such polynucleotides, and cells containing the polymerases, polynucleotides, cassettes, and/or vectors of the invention. The present invention also provides methods for synthesizing polynucleotides and kits including a dna polymerase of the invention..
Applied Biosystems, Llc

Dna polymerases and mutants thereof

The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may possess both a dna-dependent dna polymerase activity and an rna-dependent dna polymerase activity, i.e., a reverse transcriptase activity.

Compositions and methods for rt-pcr

The present invention relates to compositions and methods having propylene glycol and dna polymerase for facilitating the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of rna molecules, and for increasing the detection sensitivity and reliability through generation of secure cdna molecules prior to gene-specific primer dependent amplification. The reagent mixture comprises a ready to use reagent solution, wherein the solution comprises: (a) propylene glycol in a concentration between about 20% and about 50%; (b) a viral reverse transcriptase; and (c) at least one dna polymerases, in a buffer suitable for use in a reverse transcription reaction, wherein the buffer comprises a co-factor metal ion and nucleoside triphosphates..

Soy gene cluster regions and methods of use

Methods for conveying pathogen resistance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing pathogen resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with pathogen resistance.
Syngenta Participations Ag

Characterization of thermostable dna polymerase

Methods detecting covalent lysine modifications in dna polymerases are provided. These methods are particularly useful in determining the extent and location of a lysine modification in a dna polymerase..
Roche Molecular Systems, Inc.

Recombinase polymerase amplification

This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.
Alere San Diego, Inc.

Methods for measuring polymerase activity useful for sensitive, quantitative measurements of any polymerase extension activity and for determining the presence of viable cells

A novel, highly sensitive, quantitative and rapid dpe-pcr assay is disclosed that can be used to enumerate prokaryotic cells when presenting a purified or selected cell type and that has the capability to reproducibly measure dna polymerase extension activity from less than 10 cfu of bacteria via coupling to bead lysis. Also disclosed is the potential for the dpe-pcr assay of the invention to universally detect microbes by testing a panel of microorganisms comprised of gram-negative bacteria, gram-positive bacteria and candida species.
Zeus Scientific, Inc.

Markers associated with soybean rust resistance and methods of use therefor

Methods for conveying soybean rust (sbr) resistance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing sbr resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with sbr resistance.
Syngenta Participations Ag

Rna microchip detection using nanoparticle-assisted signal amplification

Disclosed are methods and materials for detecting rna in a sample. In some forms, the method involves (a) bringing into contact the sample and a probe array, (b) bringing into contact the probe array and a ribonuclease specific for rna/dna hybrids (such as rnase h), (c) bringing into contact the probe array, labeled nucleotides, and a nucleic acid polymerase capable of extending a rna strand using a dna template and capable of incorporating the labeled nucleotides in the extension from the rna strand (such as klenow fragment dna polymerase), and (d) detecting the labeled nucleotides in the extended nucleic acid strand.
Georgia State University Research Foundation, Inc.

Normalization of polymerase activity

Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of dna polymerases, such as taq dna polymerase.. .
Exact Sciences Corporation



Dna Polymerase topics:
  • Dna Polymerase
  • Polymerase
  • Nucleic Acid
  • Nucleotide
  • Recombinant
  • Amplification
  • Sequencing
  • Exonuclease
  • Reverse Transcriptase
  • Dna Molecule
  • Isothermal
  • Amino Acid
  • Oligonucleotide
  • Endonuclease
  • Polynucleotide


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    This listing is a sample listing of patent applications related to Dna Polymerase for is only meant as a recent sample of applications filed, not a comprehensive history. There may be associated servicemarks and trademarks related to these patents. Please check with patent attorney if you need further assistance or plan to use for business purposes. This patent data is also published to the public by the USPTO and available for free on their website. Note that there may be alternative spellings for Dna Polymerase with additional patents listed. Browse our RSS directory or Search for other possible listings.


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