Dna Polymerase

Dna Polymerase-related patent applications - as published by the U.S. Patent and Trademark Office (USPTO).

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NEW Method of diagnosing and treating epstein barr virus-based myalgic encephalomyelitis chronic fatigue syndrome patients
May 25, 2017 - N°20170146534

A method of diagnosing a subset of epstein barr virus, myalgic encephalomyelitis chronic fatigue syndrome (me/cfs) patients through a multi-prong clinical/serological analysis is provided wherein epstein barr virus abortive lytic replication (ebv) is determined as the specific causal agent through the use of serum antibodies to ebv encoded dutpase and serum antibodies to ebv dna polymerase as molecular ...
NEW Modified nucleotide reagents
May 25, 2017 - N°20170145502

Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with ...
NEW Labeled nucleotide analogs, reaction mixtures, and methods and systems for sequencing
May 25, 2017 - N°20170145496

Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with ...
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Solid phase isothermal amplification
General Electric Company
May 18, 2017 - N°20170137874

Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a dna polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dntp) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. ...
Isothermal amplification under low salt condition
General Electric Company
May 04, 2017 - N°20170121747

Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a dna polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dntp) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. ...
Dna sequencing detection field effect transistor
International Business Machines Corporation
April 27, 2017 - N°20170114402

A semiconductor structure is provided that can be used for dna sequencing detection. The semiconductor structure includes a doped epitaxial source semiconductor material structure located on a first portion of a semiconductor substrate and a doped epitaxial drain semiconductor material structure located on a second portion of the semiconductor substrate. A gate dielectric portion is located on a third portion ...
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Dna polymerases with improved activity
Roche Molecular Systems, Inc.
April 13, 2017 - N°20170101632

Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e. G., for production of the dna polymerases.
Fast pcr for str genotyping
Life Technologies Corporation
March 23, 2017 - N°20170081715

Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (str) using a ...
Method and kit of parts for extraction of nucleic acids
Ifp Privates Institut Fur Produktqualitat Gmbh
March 23, 2017 - N°20170081704

Composition, method and kit of parts for a simplified and safe extraction of nucleic acids from biological samples, such as crude and processed food, and subsequent analysis by polymerase chain reaction to the presence of animal material, genetically modified organisms, allergens and pathogens. The method comprises the addition of extracting composition together with hot water for extraction and stabilization of ...
Dna polymerases
Thermo Fisher Scientific Baltics Uab
March 23, 2017 - N°20170081646

A dna polymerase mutant comprising a taq dna polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: e189k, e230k, e507k, h28r, l30r, g38r, f73v, h75r, e76a, e76g, e76k, e90k, k206r, e315k, a348v, l351f, a439t, ...
Method and reagent for enrichment of circulating tumor dna
Haplox Biotechnology(shenzhen) Co., Ltd.
March 09, 2017 - N°20170067117

The present invention provides a method and a reagent for enrichment of circulating tumor dna, the method comprising the steps of mixing a water phase and an oil phase and shaking the mixture to prepare an emulsion pcr reaction system, and performing emulsion pcr amplification, wherein the water phase comprises peripheral blood plasma dna as template dna, a forward primer ...
Recombinant polymerases with increased phototolerance
Pacific Biosciences Of California, Inc.
March 09, 2017 - N°20170067038

Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic ...
Modified dna polymerases for improved amplification
Kapa Biosystems
March 09, 2017 - N°20170067037

The present invention provides improved dna polymerases that may be better suited for applications in recombinant dna technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified dna polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.
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Modified type a dna polymerases
Kapa Biosystems, Inc.
February 23, 2017 - N°20170051326

The present invention provides improved dna polymerases, in particular, type a dna polymerases, that may be better suited for applications in recombinant dna technologies. Among other things, the present invention provides modified dna polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.
Compositions for recombinase polymerase amplification
Alere San Diego, Inc.
February 16, 2017 - N°20170044598

This disclosure describe three related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of the bacterial reca and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods has the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity ...
Methods for temperature-mediated nested polymerase chain reaction
Tetracore, Inc.
February 16, 2017 - N°20170044585

Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a ...
Methods and compositions for inhibition of polymerase
Biocryst Pharmaceuticals, Inc.
February 16, 2017 - N°20170042901

The present invention is directed to methods and compositions for inhibition of viral nucleic acid polymerases, such as rna and dna polymerases, and methods and compositions that are useful for treating viral infections in subjects. The methods comprise administering to the subject a therapeutically effective amount of a compound of formula i, or a pharmaceutically acceptable salt or hydrate thereof, ...
Kit and method
Biocryst Pharmaceuticals, Inc.
February 02, 2017 - N°20170029867

The present invention relates to a kit comprising a dna-dependant dna polymerase and at least one natural deoxynucleoside and to a kit comprising a dna-dependent dna polymerase and a detection system comprising a dna template molecule, a dna primer molecule, and a fluorescent moiety capable of being displaced from, or bound to, dsdna synthesized by said dna-dependent polymerase. It further ...
Dna polymerases from the red sea brine pool
King Abdullah University Of Science And Technology
February 02, 2017 - N°20170029793

A dna polymerase composition for amplifying nucleic acids can be tolerant of extreme conditions.
Dna polymerases with improved activity
Roche Molecular Systems, Inc.
February 02, 2017 - N°20170029792

Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e. G., for production of the dna polymerases.
Dna polymerases with improved activity
Roche Molecular Systems, Inc.
February 02, 2017 - N°20170029791

Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e. G., for production of the dna polymerases.