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Dna Polymerase patents

      

This page is updated frequently with new Dna Polymerase-related patent applications.




 Method and reagent for enrichment of circulating tumor dna patent thumbnailMethod and reagent for enrichment of circulating tumor dna
The present invention provides a method and a reagent for enrichment of circulating tumor dna, the method comprising the steps of mixing a water phase and an oil phase and shaking the mixture to prepare an emulsion pcr reaction system, and performing emulsion pcr amplification, wherein the water phase comprises peripheral blood plasma dna as template dna, a forward primer and a reverse primer, dntps, a pcr buffer and a dna polymerase, the peripheral blood plasma dna having adapter sequences connected to both ends thereof, and the forward primer and the reverse primer being complementary to the adapter sequences at the two ends respectively; separating the water phase from the oil phase following the emulsion pcr amplification to obtain a pcr amplification product in the water phase; and capturing circulating tumor dna in the pcr amplification product in the water phase by using a probe sequence that specifically binds to the circulating tumor dna. The method of the present invention is capable of performing single-molecule high-fidelity ultramicro parallel amplification and effectively capturing peripheral blood plasma ctdna to provide adequate amount of ctdna to be used for subsequent sequencing detection..
Haplox Biotechnology(shenzhen) Co., Ltd.


 Recombinant polymerases with increased phototolerance patent thumbnailRecombinant polymerases with increased phototolerance
Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like.
Pacific Biosciences Of California, Inc.


 Modified dna polymerases for improved amplification patent thumbnailModified dna polymerases for improved amplification
The present invention provides improved dna polymerases that may be better suited for applications in recombinant dna technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified dna polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications..
Kapa Biosystems


 Method of determining the nucleotide sequence of oligonucleotides and dna molecules patent thumbnailMethod of determining the nucleotide sequence of oligonucleotides and dna molecules
The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection.
Life Technologies Corporation


 Modified type a dna polymerases patent thumbnailModified type a dna polymerases
The present invention provides improved dna polymerases, in particular, type a dna polymerases, that may be better suited for applications in recombinant dna technologies. Among other things, the present invention provides modified dna polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications..
Kapa Biosystems, Inc.


 Compositions for recombinase polymerase amplification patent thumbnailCompositions for recombinase polymerase amplification
This disclosure describe three related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of the bacterial reca and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods has the advantage of not requiring thermocycling or thermophilic enzymes.
Alere San Diego, Inc.


 Methods for temperature-mediated nested polymerase chain reaction patent thumbnailMethods for temperature-mediated nested polymerase chain reaction
Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a first temperature and cool the reaction mixture to a second temperature repeatedly for a first plurality of cycles.
Tetracore, Inc.


 Methods and compositions for inhibition of polymerase patent thumbnailMethods and compositions for inhibition of polymerase
The present invention is directed to methods and compositions for inhibition of viral nucleic acid polymerases, such as rna and dna polymerases, and methods and compositions that are useful for treating viral infections in subjects. The methods comprise administering to the subject a therapeutically effective amount of a compound of formula i, or a pharmaceutically acceptable salt or hydrate thereof, or a composition comprising a compound of formula i, or a pharmaceutically acceptable salt or hydrate thereof, and a pharmaceutically acceptable carrier.
Biocryst Pharmaceuticals, Inc.


 Kit and method patent thumbnailKit and method
The present invention relates to a kit comprising a dna-dependant dna polymerase and at least one natural deoxynucleoside and to a kit comprising a dna-dependent dna polymerase and a detection system comprising a dna template molecule, a dna primer molecule, and a fluorescent moiety capable of being displaced from, or bound to, dsdna synthesized by said dna-dependent polymerase. It further relates to a method for measuring deoxynucleoside kinase activity in a sample characterized by; (i) contacting the sample, in a container, with a reaction mix comprising a dna-dependent dna polymerase, at least one deoxynucleoside and a detection system comprising a dna template molecule, a dna primer molecule, and a fluorescent moiety capable of being incorporated in, displaced from, or bound to dsdna synthesized by said dna dependent polymerase; (ii) incubating said container; (iii) measuring the signal from the fluorescent moiety; and correlating the signal from the fluorescent moiety to the deoxynucleoside kinase activity in the sample..

 Dna polymerases from the red sea brine pool patent thumbnailDna polymerases from the red sea brine pool
A dna polymerase composition for amplifying nucleic acids can be tolerant of extreme conditions.. .
King Abdullah University Of Science And Technology


Dna polymerases with improved activity

Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Dna polymerases with improved activity

Disclosed are dna polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Phi29 dna polymerase mutants having increased thermostability and processivity

Mutants of bacteriophage phi29 dna polymerase with increased protein stability and increased half-life, compared to wild type dna polymerase. The disclosed mutants are more stable in reaction mixtures with or without dna.
Thermo Fisher Scientific Baltics Uab

Novel methods of identifying patients responsive to immunotherapeutic strategies

The invention includes a method of determining whether a mammal's cancerous tumor is associated with a hypermutator phenotype (i.e., harboring a large number of mutations) for the dna polymerase epsilon (pole) gene as compared to normal cells. The invention further includes a method of selecting patients harboring an immunogenic tumor that is responsive to immunotherapy..
Yale University

Compositions, kits and methods for synthesis and/or detection of nucleic acids

A composition comprising a thermostable dna polymerase; and a pcr inhibitor blocking agent, wherein the pcr inhibitor blocking agent is present in an amount effective to enhance tolerance of an assembled pcr to a pcr inhibitor.. .
Life Technologies Corporation

Methods for beaming

Improvements on the basic method used for beaming increase sensitivity and increase the signal-to-noise ratio. The improvements have permitted the determination of intrinsic error rates of various dna polymerases and have permitted the detection of rare and subtle mutations in dna isolated from plasma of cancer patients..
The Johns Hopkins University

Dna polymerase possessing continuous catalytic capacity and salt tolerance

A dna polymerase, having an amino acid sequence represented by seq id no. 2, or a derivative of the amino acid sequence by substitution, deletion, or addition of at least one amino acid residue.
Biotechnology Research Institute, Chinese Academy Of Agricultural Sciences

Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process

The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step.
Rubicon Genomics, Inc.

Recombinant polymerases for incorporation of protein shield nucleotide analogs

Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing or nucleic acid amplification. Such properties include enhanced performance with large nucleotide analogs, increased stability, increased readlength, and improved detection of modified bases, and can also include resistance to photodamage, enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased accuracy, altered speed, increased cosolvent resistance, and the like.
Pacific Biosciences Of California, Inc.

Method for testing a mutant gene through real time polymerase chain reaction using inhibition of 5'-flap endonuclease activity

The present invention relates to a method for detecting single nucleotide polymorphism (snp) using a feature that the 5′-flap endonuclease (fen) activity of dna polymerase is inhibited when a probe complementarily binds to the end of a polymerase chain reaction (pcr) product. More specifically, the present invention relates to a novel method wherein it was verified that, when a probe used for a real-time pcr complementarily binds to the end site of a pcr product, the 5′-fen activity of thermostable dna polymerase to the probe is inhibited, and thus when such a feature is used to make a design such that an snp site to be detected is located at the 5′-end site of the probe, the 5′-flap formation is induced according to the allele, thereby allowing effective snp detection..
Genotech Corp.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Mutant rb69 dna polymerase

Provided herein are mutant dna-dependent polymerases which are derived from, or otherwise related to, wild type rb69 dna polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides.
Life Technologies Corporation

Methods for genotyping

The present invention provides for methods for discriminating between alleles at polymorphic positions in a genome. In general the methods employ allele specific extension of oligonucleotides that are complementary to one of the alleles at the 3′ end of the oligonucleotide.
Affymetrix, Inc.

High-throughput sequencing detection methylated cpg islands

A high-throughput sequencing method for detecting methylated cpg islands includes: processing a dna sample by using a modifier, and converting cytosine in the dna sample into uracil, and keeping 5′methylcytosine unchanged; amplifying the obtained segment by using a primer a and dna polymerase, to obtain a segment having one end being capable of anchoring a junction primer c; amplifying the obtained segment by using a primer b and dna polymerase, to obtain a segment gathering methylated cpg islands and having two ends being capable of separately anchoring junction primers c and d; amplifying the obtained segment at a pcr exponent by using the junction primers c and d and the dna polymerase, to obtain the amplified product; and separating and purifying the amplified product, to form a high-throughput sequencing library and perform computer sequencing, and data analysis.. .
Peking University

Mutated dna polymerases with high selectivity and activity

The present invention provides dna polymerases and their use in various applications. The present invention also relates to uses and methods utilizing the dna polymerase of the present invention for discriminating between matched and mismatched primers or detecting snp or methylated cytosine.
Universitaet Konstanz

Novel compounds useful for the treatment of bacterial infectious diseases

Novel compounds of the invention may be prepared as a pharmaceutical composition, and may be useful in the treatment of infectious diseases, in particular bacterial infectious diseases. The compounds may be active against a specific enzyme in the bacterial dna replicative process, dna polymerase iiie..

Methods and compositions for repair of dna ends by multiple enzymatic activities

Provided herein are compositions for and methods of generating ligation-competent nucleic acids. In some aspects, the compositions comprise exonuclease iii, t4 dna polymerase, klenow, and/or t4 polynucleotide kinase..
Rubicon Genomics, Inc.

Isothermal amplification using oligocation-conjugated primer sequences

Provided herein are methods and kits for isothermal nucleic acid amplifications that use an oligocation-oligonucleotide conjugate primer for amplifying a target nucleic acid to generate amplicons. Isothermal dna amplification methods that employ a strand displacing dna polymerase and polyamine-oligonucleotide conjugate primer are also provided..
General Electric Company

Recombinase polymerase amplification

This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.
Alere San Diego Inc.

Methods and compositions for interference with dna polymerase and dna synthesis

Disclosed are methods and compositions for inhibiting dna synthesis in a cell using rna. Inhibition of dna synthesis by rna can be used, for example, in analytical methods, as a research tool to affect cells under study, to synchronize cell cycle in a cell culture, and to inhibit cell growth.
Georgia State University Research Foundation, Inc.

Mutant dna polymerase blends and mutant dna polymerases

A thermostable dna polymerase composition comprising at least two dna polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, dna sequencing, nucleic acid amplification and cdna synthesis..
Life Technologies Corporation

Enzyme preparation containing thermostable dna polymerase, producing same, and detecting subject organism to be detected

Disclosed is a thermostable dna polymerase preparation which can illimitably reduce the risk of false positivity in the detection of a subject microorganism utilizing a gene amplification reaction and therefore enables the selective amplification of dna for detecting the subject microorganism even when the amount of the subject microorganism is small and therefore the amount of dna collected therefrom is extremely small, and can be produced at a reduced cost. Also disclosed is a method for quantifying or quantifying/identifying a subject organism to be detected rapidly, conveniently and with high sensitivity using the preparation of the present invention..
National University Corporation University Of Toyama

Recombinant polymerases with increased phototolerance

Provided are compositions comprising recombinant dna polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like.
Pacific Biosciences Of California, Inc.

Methods and compositions for rapid seamless dna assembly

Provided herein are methods for assembling dna fragments employing at least three enzymatic activities: dna polymerase, flap endonuclease, and dna ligase. Certain aspects include methods for generating closed circular dna products, e.g., plasmid vectors, by assembling various dna fragments having complementary ends that hybridize to one another.
Agilent Technologies, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Modified polymerase compositions, methods and kits

This invention relates to type i dna polymerases, for example, taq dna polymerase, and to methods and reagent kits utilizing such polymerases.. .
Brandeis University

Integrated on chip detector and zero waveguide module structure for use in dna sequencing

A semiconductor structure for use in single molecule real time dna sequencing technology is provided. The structure includes a semiconductor substrate including a first region and an adjoining second region.
International Business Machines Corporation

Nucleic acid amplification method using allele-specific reactive primer

The detection method using the allele-specific reactive primer (asrp) according to the present invention is a technique having a very high specificity of amplification due to the characteristics of the asrp and a proofreading dna polymerase. The detection method can effectively detect mutations (point mutation, insertion, deletion, etc.) including a single nucleotide polymorphism (snp), and can also be used to detect methylation of cpg after bisulfite treatment or to amplify and detect a target dna starting with a desired nucleotide sequence from a dna library..

Ph measurement for sequencing of dna

The present method involves sequencing by synthesis in which a template strand having an attached primer is immobilized in a small volume reaction mixture. In one embodiment, the reaction mixture is in contact with a sensitive heat sensor, which detects the heat of reaction from incorporation of a complementary base (dntp) in the presence of appropriate reagents (dna polymerase, and polymerase reaction buffer).

A the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides

Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during dna polymerase-mediated replication of dna or rna polymerase-mediated transcription of rna. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded dna or rna), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker..
Synthorx, Inc.

Mutant endonuclease v enzymes and applications thereof

Provided herein are mutant endonuclease v enzymes that are capable of nicking an inosine-containing dna sequence. Nucleic acid assays and agents that employ such mutant endonuclease v enzymes to introduce a nick into a target dna including one or more inosine, and uses a dna polymerase to generate amplicons of a target dna are also described..
General Electric Company

Recombinant reverse transcriptases

The present invention relates to a gene that encodes a hyperactive reverse transcriptase having dna polymerase activity and substantially reduced rnase h activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cdna from mrna using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase..
Applied Biosystems, Llc

Fusion polymerase and using the same

This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a dna polymerase; and (b) a heterologous sequence-specific dna binding domain. A method for copying a dna template, as well as a kit for performing the same, are also described..
New England Biolabs, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.

Dna polymerases with increased 3'-mismatch discrimination

Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
Roche Molecular Systems, Inc.



Dna Polymerase topics:
  • Dna Polymerase
  • Polymerase
  • Nucleic Acid
  • Nucleotide
  • Recombinant
  • Amplification
  • Sequencing
  • Exonuclease
  • Reverse Transcriptase
  • Dna Molecule
  • Isothermal
  • Amino Acid
  • Oligonucleotide
  • Endonuclease
  • Polynucleotide


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    This listing is a sample listing of patent applications related to Dna Polymerase for is only meant as a recent sample of applications filed, not a comprehensive history. There may be associated servicemarks and trademarks related to these patents. Please check with patent attorney if you need further assistance or plan to use for business purposes. This patent data is also published to the public by the USPTO and available for free on their website. Note that there may be alternative spellings for Dna Polymerase with additional patents listed. Browse our RSS directory or Search for other possible listings.


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