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Chromatography patents

      

This page is updated frequently with new Chromatography-related patent applications.




 Method for preparing a small-diameter chromatography column patent thumbnailnew patent Method for preparing a small-diameter chromatography column
Packing a column with a chromatography medium at prescribed compression factor, and then passing a mobile phase through the column packed with the chromatography medium while switching the mobile phase flow direction two or more times. According to the present invention, a small-diameter chromatography column packed uniformly and densely as well as a large-diameter column can be reproducibly prepared.
Kaneka Corportion


 Autosampler and gas chromatography system and method including same patent thumbnailnew patent Autosampler and gas chromatography system and method including same
A gas chromatography system includes at least one gas chromatography subsystem including at least one injector port, and an autosampler. The autosampler includes a carousel tray mounted for rotation about a rotation axis and including arcuately extending first and second rows of sample reservoirs, a first sample transfer tower to extract samples from the first row, a second sample transfer tower to extract samples from the second row, and a control system operative to: selectively position the carousel tray relative to the first and second sample transfer towers to align the first and second sample transfer towers with a selected pair of the sample reservoirs of the first and second rows, respectively; draw samples from the selected pair using the first and second sample transfer towers; inject the sample drawn from the first row into the at least one injector port using the first sample transfer tower; and inject the sample drawn from the second row into the at least one injector port using the second sample transfer tower..
Perkinelmer Health Sciences, Inc.


 Method for purifying and quantifying thrombin and its degradation polypeptides patent thumbnailnew patent Method for purifying and quantifying thrombin and its degradation polypeptides
Provided is a method for purifying α-thrombin and for quantifying α-thrombin and its degradation polypeptides in a liquid proteinatious solution. The method employs a one-step anion exchange chromatography method.
Omrix Biopharmaceuticals Ltd.


 Chromatography membranes formed by thiol-ene or thiol-yne click polymerization reactions patent thumbnailnew patent Chromatography membranes formed by thiol-ene or thiol-yne click polymerization reactions
Disclosed are composite materials and methods of making them. The composite materials comprise a support member and a cross-linked gel, wherein the cross-linked gel is a polymer synthesized by thiol-ene or thiol-yne polymerization and cross-linking.
Natrix Separations Inc.


 Method for production of a chromatography material patent thumbnailnew patent Method for production of a chromatography material
The present invention relates to a method for production of a chromatography material. More closely, the invention relates to a method for production of a reverse phase chromatography (rpc) material comprising the following steps: introduction of unsaturated groups onto porous carbohydrate particles and grafting of styrenic monomers on said particles comprising an unsaturated group..
Ge Healthcare Bio-sciences Ab


 Systems and methods for characterization of molecules patent thumbnailSystems and methods for characterization of molecules
The present invention generally provides systems and methods for the detection, identification, or characterization of differences between properties or behavior of corresponding species in two or more mixtures comprised of molecules, including biomolecules and/or molecules able to interact with biomolecules, using techniques such as partitioning. The experimental conditions established as distinguishing between the mixtures of the molecules using the systems and methods of the invention can also be used, in some cases, for further fractionation and/or characterization of the biomolecules and/or other molecules, using techniques such as single-step or multiple-step extraction, and/or by liquid-liquid partition chromatography.
Analiza, Inc.


 Systems and methods for two-dimensional chromatography patent thumbnailSystems and methods for two-dimensional chromatography
Provided are two-dimensional chromatography systems and methods for separating and/or analyzing complex mixtures of organic compounds. In particularly, a two-dimensional reversed-phase liquid chromatography (rplc)-supercritical fluid chromatography (sfc) system is described including a trapping column at the interface which collects the analytes eluted from the first dimension chromatography while letting the rplc mobile phase pass through.
Genentech, Inc.


 Method for detecting and quantifying galacto-oligosaccharide patent thumbnailMethod for detecting and quantifying galacto-oligosaccharide
A method for detecting and quantifying galacto-oligosaccharide in a sample containing galacto-oligosaccharide and dextrin is characterized in that the sample is caused to react with a derivatizing reagent to derivatize the dextrin and galacto-oligosaccharide in the sample, after which the galacto-oligosaccharide component in the sample is separated by high-performance liquid chromatography using a c30 reverse-phase chromatography column, thereby accurately detecting and quantifying galacto-oligosaccharide with ease and at low cost.. .
Kabushiki Kaisha Yakult Honsha


 Cation and anion exchange chromatography method patent thumbnailCation and anion exchange chromatography method
Herein is reported a method for purifying a polypeptide comprising the steps of i) applying a solution comprising the polypeptide to an ion exchange chromatography material, and ii) recovering the polypeptide with a solution comprising a denaturant and thereby purifying the polypeptide, whereby the ion exchange chromatography material comprises a matrix of cross-linked poly (styrene-divinylbenzene) to which ionic ligands have been attached, and wherein the solution comprising the polypeptide applied to the ion exchange chromatography material is free of the denaturant and the polypeptide adsorbed to the ion exchange chromatography material is recovered with a solution comprising a denaturant at a constant conductivity.. .
Hoffmann-la Roche Inc.


 Alternative nucleic acid molecules containing reduced uracil content and uses thereof patent thumbnailAlternative nucleic acid molecules containing reduced uracil content and uses thereof
The present disclosure provides alternative nucleosides, nucleotides, and nucleic acids, and methods of using them. In some aspects, the disclosure provides mrna wherein the uracil content has been modified and which may be particularly effective for use in therapeutic compositions, because they may benefit from both high expression levels and limited induction of the innate immune response.
Moderna Therapeutics, Inc.


Kind of mutated proteins a with high alkali resistance feature and application thereof


A series of protein a mutants having high alkali resistance, and methods of using the protein a mutants are provided. The protein a mutants have a high binding affinity for regions of immunoglobulin proteins other than the complementarity determining regions.
Nanjing Genscript Co., Ltd.


Purification of organic compounds by surfactant mediated preparative hplc


A sample is provided that can be purified by preparative reversed phase high performance liquid chromatography (prep-rp-hplc) in a single run in spite of recent advances in the production of reversed phase derivatized silica stationary supports: (1) the traditional approach is to use a bigger column (greater amount of stationary phase); and (2) use displacement chromatography which (while labor intensive to develop) uses the stationary phase more effectively. This disclosure describes a unique prep-rp-hplc technique that uses a c-18/c-8 derivatized silica coated with a surfactant such as triton x-100 to result in 7 to 10 fold increase in sample loading (of the crude mixture of organic compounds including synthetic crude peptides) in contrast to the conventional prep-rp-hplc technique.
Davuluri, Ramamohan Rao


Method for preparing abiraterone acetate


A method for preparing abiraterone acetate. The steps are: dehydroepiandrosterone acetate and trifluoromethanesulphonic anhydride undergo a sulfonylation reaction under the catalysis of an organic base to obtain a compound as represented by formula ii; the compound is reacted with a 3-pyridine organoboron compound or a 3-pyridine organosilicone compound under the catalysis of bis(triphenylphosphine) palladium(ii) dichloride to obtain a crude abiraterone acetate product; the crude product is recrystallized in a protic or aprotic solvent to obtain an abiraterone acetate crystal; the crystal is further put into a solvent which easily dissolves the crystal and dissolved under heating, and the solution is dropwise added into a solvent which does not easily dissolve the crystal until a solid is precipitated under stirring, such that a micro powder abiraterone acetate is obtained; and the solvent which easily dissolves the crystal is a mixture of any two or more of acetone, ethanol and water, and the solvent which does not easily dissolve the crystal is water.
Wuhan Biocause Pharmaceutical Co., Ltd.


Process for purification of neutral human milk oligosaccharide using simulated moving bed chromatography


The present application discloses a process for the purification of a neutral human milk oligosaccharide (neutral hmo). The process uses simulated moving bed (smb) chromatography which allows the continuous purification of large quantities of hmos with high purity.
Jennewein Biotechnologie Gmbh


System for liquid chromatograph with compressibility and viscosity monitoring to identify fluids


A system for providing a solvent or reagent to a liquid chromatography system comprises: a valve comprising a common port and a plurality of other ports, configurable such that the common port may be fluidically coupled to any one of the other ports; a pump fluidically coupled to the common port of the valve; a plugged first one of the other ports; a container containing the solvent or reagent, said container fluidically coupled to a second one of the other ports; a fluid tubing line having a known resistance to fluid flow fluidically coupled to a third one of the other ports; and a pressure gauge configured to measure fluid pressure within the pump, wherein the solvent or reagent is provided to the liquid chromatography system by a fourth one of the other ports.. .
Thermo Finnigan Llc


Automated adjustment of capillary voltage based on the elution conditions to retain optimal ionization conditions


A method of electrospray ionisation is disclosed comprising passing a sample liquid through a liquid chromatography column, monitoring a liquid chromatography back pressure and varying a voltage applied to an electrospray ionisation source electrode in dependence upon said monitored liquid chromatography back pressure.. .
Micromass Uk Limited


Multidrug analysis in urine by liquid chromatography-tandem mass spectrometry


A fast and reliable method for the determination of multiple drugs and their metabolites belonging to different chemical and toxicological class from a biological sample is provided. The method involves mixing of biological sample with internal standards which does not require sample extraction or derivatization prior to analysis.
Castle Medical, Llc


Device and methods of using device for detection of hyperammonemia


The present disclosure relates to a bio-sensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkaline buffer in a vessel configured to receive a sample, such as whole blood.
The United States Of America, As Represented By The Secretary, Dept., Of Health And Human Services


Base station for liquid chromatography analysis unit


A base station for separating a fluidic sample by liquid chromatography in cooperation with a liquid chromatography analysis unit configured for executing a predefined liquid chromatography analysis task, wherein the base station comprises an analysis unit coupling section configured for removably coupling the liquid chromatography analysis unit to the base station, and an analysis task identification unit configured for identifying the predefined liquid chromatography analysis task of the liquid chromatography analysis unit coupled to the analysis unit coupling section, so that the fluidic sample is separable by executing the identified predefined liquid chromatography analysis task in cooperation between the base station and the liquid chromatography analysis unit.. .
Agilent Technologies, Inc.


Liquid chromatography analysis unit


A liquid chromatography analysis unit configured for executing a predefined liquid chromatography analysis task, wherein the liquid chromatography analysis unit comprises a sample insertion compartment configured for inserting a fluidic sample to be separated, wherein the fluidic sample is to be separated when executing the liquid chromatography analysis task, a solvent accommodation section accommodating a predefined volume of at least one predefined solvent required for executing the liquid chromatography analysis task, and a sample separation unit configured for separating the fluidic sample inserted into the sample insertion compartment upon applying a solvent and sample drive force for driving the solvent and the fluidic sample through the sample separation unit.. .
Agilent Technologies, Inc.


Liquid chromatography columns with structured walls


A liquid chromatography (lc) column includes a wall having a length along a central axis from the inlet end to the outlet end, the wall enclosing a column interior and having a column radius relative to the central axis, the wall comprising a structured portion configured such that the column radius varies along the length; and a plurality of particles packed in the column interior, wherein at least some of the particles are in contact with the structured portion.. .
Agilent Technologies, Inc.


Portable fluidic platform for rapid cell-free production of protein biologics


A portable fluidic platform for rapid and flexible end-to-end production of recombinant protein biologics includes a bioreactor system hosting stable and robust cell-free translation systems that is fluidically integrated with modular protein separation functionalities (e.g., size exclusion, ion exchange or affinity chromatography systems) for purification of the cell-free expressed product and which are configurable for process-specific isolation of different proteins, as well as for formulation. The bioreactor utilizes lysates from engineered eukaryotic (e.g., yeast) or prokaryotic (e.g., bacterial) strains that contain factors for protein folding and posttranslational modifications.
Leidos, Inc.


E. coli separatome-based protein expression and purification platform


Provided is a separatome-based peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome of e. Coli.
Board Of Trustees Of The University Of Arkansas


R type of resveratrol dimer, preparation process therefor and purpose thereof in lowering blood sugar level


The present invention relates to the field of natural pharmaceutical chemistry, and in particular, to a resveratrol dimer (7r,8r)-trans-δ-viniferin (i), a preparation process therefor and a purpose thereof in lowering a blood sugar level. According to the present invention, an r type of resveratrol dimer is separated from the resveratrol dimer by using high-speed countercurrent chromatography.
China Pharmaceutical University


Chromatography column assembly


The invention relates to a chromatography column assembly that offers an affordable self-packed column for performing chromatographic separation. The chromatography column assembly comprises a column tube having a first and a second opening at substantially opposite ends, and an end cap of either a snap fit design or a bolted design.
Ge Healthcare Bio-science Ab


System and assembling a large scale chromatography structure


A container system is disclosed, where the container system includes a housing. A chromatography cartridge is disposed in the housing.
Ge Healthcare Biosciences Corp.


Method for transferring slurry


A method and a slurry tank system for transferring chromatography media slurry from a slurry tank (3) to a chromatography column (9), said method comprising monitoring the content of slurry in the slurry tank (3) during the transferring and controlling the transferring of the slurry to the column (9) in dependence of the content of slurry in the slurry tank (3).. .
Ge Healthcare Bio-sciences Ab


Column contained in container and column container


Provided is a chromatography column stored in a storage container. The storage container for the column includes: a container body for storing the chromatography column; and a cover threadedly engaging with the container body.
Sekisui Medical Co., Ltd.


Isolation, detection and use of biological cells


This invention relates to devices and methods for purifying, detecting and using biological cells. A variety of cell types including viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column.

Low carryover high pressure fluidic fitting


Described is a fitting for coupling fluidic paths which facilitates secure and low leak rate fluid flow through a fluidic path junction such as a junction in a liquid chromatography system or other chemical analysis instrument. Fluid containing various mixtures of chemical samples can pass through the junction at high pressure with little or no trapped residue remaining in a volume at the connection.
Waters Technologies Corporation


Purification embryo - derived infectious bronchitis virus (lbv)


The invention discloses an avian infectious bronchitis virus purification method. The method uses ordinary avian embryo which was affected by avian bronchitis virus.
South China Agricultural University


Compounds for affinity chromatography and for extending the half-life of a therapeutic agent


Compounds useful for affinity chromatography as presented, more particularly for use in affinity chromatography to purify serum albumin, especially human serum albumin (hsa) and fusion proteins thereof. Methods for extending the half-life of therapeutic agents are also presented, particularly therapeutic peptide agents and small molecules, such as by conjugation of compounds described herein to the therapeutic peptide or small molecule, which, upon administration, binds to hsa, thereby providing a prolonged release of the therapeutic agent..
Glaxosmithkline Intellectual Property Development Limited


Probe assembly for attaching a chromatography device to a mass spectrometer


A probe assembly is disclosed comprising an inlet for receiving an eluent from a chromatography device; an outlet (120) for delivering the eluent to an ion source of a mass spectrometer; and an attachment device (122) for attaching the outlet to the mass spectrometer. The outlet comprises an electrically conductive capillary (124) and an electrically conductive member (129) surrounding at least part of the electrically conductive capillary (124).
Micromass Uk Limited


Automated sterile processing of cells by centrifugation and column chromatography


The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.. .
Miltenyi Biotec Gmbh


Optical pressure-sensitive adhesive sheet


An optical pressure-sensitive adhesive sheet for silver nanowire layer use includes a pressure-sensitive adhesive layer. The amount of acrylic acid ions extracted from the pressure-sensitive adhesive layer with pure water at 100° c.
Nitto Denko Corporation


Micro chiral regulation cellulose chromatography stationary phase, preparation method and use thereof


The present invention discloses a cellulose derivative shown as formula (i), which is obtained as follows: the hydroxyl at position 6 of microcrystalline cellulose is protected with triphenylchloromethane, and then reacted with acyl chloride or isocyanate. After the protection of the hydroxyl at positions 2 and 3 of microcrystalline cellulose, triphenylmethyl is removed under acidic conditions to expose the hydroxyl at position 6.
Beijing Dima Outai Development Center Of Science And Technology


Multimeric immunoglobulin-binding domain


A multimeric immunoglobulin-binding protein having improved properties as an affinity ligand for affinity chromatography, and an insoluble support inmmobilizing such a multimer. The immunoglobulin-binding protein is represented by the formula: (r1)n-(r2)m, or (r2)m-(r1)n.
Protenova Co., Ltd.


Chromatography column


A chromatography column having a longitudinal axis and comprising a column wall with a first end and a second end, a first end plate assembly removably connectable to said first end of the column wall, a second end plate assembly removably connectable to said second end of the column wall, wherein said first end plate assembly, said column wall and said second end plate assembly are arranged along the longitudinal axis of the column wherein the column wall, and/or first end plate assembly and/or second end plate assembly is/are rotatable about an axis of rotation wherein said axis of rotation is parallel to the longitudinal axis of said column and positioned outside the column.. .
Ge Healthcare Bio-sciences Ab


Moveable chromatography column separator


The current invention is directed to a chromatography column separator which separates the chromatography column in an upper chromatography column chamber and a lower chromatography column chamber, and which has a variable position within the chromatography column, and which is embedded by the chromatography material. The separator allows the replacement of the chromatography material in the upper chromatography column chamber without the need to replace the chromatography column material in the lower chromatography column chamber and it allows also the combination of two different chromatography materials with different chromatographical functional groups in one chromatography column..
Hoffmann-la Roche Inc.


Test piece for immunochromatography, developing fluid used therefor, and immunochromatography using the same


A test piece for immunochromatography, containing an aggregation inhibiting pad, a conjugate pad, and a membrane, in which the membrane contains a test area for capturing a target substance, the aggregation inhibiting pad contains a desalting agent, and the conjugate pad contains a labeling agent.. .
Furukawa Electric Co., Ltd.


Beverage test apparatus


A beverage test apparatus, including a container, for containing beverage to be tested; an opening disposed on the container, the opening being covered by a one-way valve, for allowing entrance of the beverage into the container, and for disallowing exit therefrom, and a chromatography substance contained in the container, for changing a color of the chromatography substance upon presence of a certain ingredient, thereby a portion of beverage poured into the cup enters the container and is tested by the chromatography substance, and thereby the one-way valve disallows access of the chromatography substance to the beverage being drunk by the user, where the beverage test apparatus may further include a sticker attached to the container, for sticking the container to the drinking cup.. .

Method for the isolation and purification of anhydrogalactose


The present invention discloses a method for isolating and purifying anhydrogalactose. Using the method for isolating and purifying anhydrogalactose including the steps of preparing a sugar mixture containing anhydrogalactose produced through chemical synthesis and hydrolysis; and isolating anhydrogalactose by performing recycling preparative liquid chromatography (recycling-prep-lc) with the sugar mixture, highly pure anhydrogalactose can be efficiently isolated and purified in large amounts..
Biolsystems Co., Ltd.


Polystyrene-containing composite resin particles and producing same, expandable composite resin particles, pre-expanded particles, and expanded molded article


Polystyrene-based composite resin particles includes 100 to 500 parts by weight of a polystyrene-based resin with respect to 100 parts by weight of an ethylene-vinyl acetate copolymer resin. The polystyrene-based composite resin particles are impregnated with a volatile blowing agent and are pre-expanded to obtain pre-expanded particles; the obtained pre-expanded particles are immersed in tetrahydrofuran for 24 hours to obtain an extract a; and the pre-expanded particles are divided into two halves through the center to prepare halved particles, and the halved particles are immersed in tetrahydrofuran for 24 hours to obtain an extract b; and the obtained extract a and extract b are subjected to a gpc (gel permeation chromatography) measurement and obtain the following results: the polystyrene-based resin having a weight-average molecular weight (mw) of 100,000 to 500,000 gives a peak obtained from chromatograms for the extract a and for the extract b..
Sekisui Plastics Co., Ltd.


Purified cardiogenin isomer and related methods


A cardiogenin major isomer is obtained from a methanol extract of geum japonicum and separated from its minor isomer. The separation of the two isomers can be achieved by chiral phase chromatography, e.g., using a chiralpak® ic™ column.
Huya Bioscience International Llc


Preparative chromatography column


A chromatography column design and a packing device, as well as a method for obtaining a column with a very compact and uniform bed are provided. The bed remains compact and uniform during operation of the column and during sanitization, storage and transportation of the column.
Bio-rad Laboratories, Inc.


Supercritical fluid-liquid chromatograph, and analysis method thereof


A back pressure valve configured to switch between a pressurized state and a released state open to an atmosphere is used as a back pressure valve in a supercritical fluid chromatograph. Switching from a state of supercritical fluid chromatography analysis to liquid chromatography is performed by stopping a pump that supplies a supercritical fluid, continuously operating a pump that supplies a solvent used as a modifier, and bringing the back pressure valve into the released state..
Shimadzu Corporation


Chromatography ligand comprising domain c from staphylococcus aureus protein a for antibody isolation


The present invention relates to a chromatography ligand, which comprises domain c from staphylococcus protein a (spa), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (cip) conditions, and is capable of binding fab fragments of antibodies.
Ge Healthcare Bio-sciences Ab


Purification process for pth


The present invention relates to improved method for purification of a recombinant parathyroid hormone (rhpth1-34 or teriparatide), said process for purification of parathyroid hormone comprising following essential steps: (a) enzymatic cleavage; (b) anion exchange chromatography, followed by other suitable purification steps; wherein step (a) and (b) can be carried out in any order.. .
Cadila Healthcare Limited


Buoyant protein harvesting device


A buoyant device containing chromatography media performs the function of protein harvesting replacing the steps of cell separation and volume reduction; the device can be loaded into columns for further purification.. .
Therapeutic Proteins International, Llc


Process for separating a divinyl hydrocarbon from monovinyl hydrocarbons and/or non-vinyl compounds


A process for separating a divinyl hydrocarbon from a composition mixture stream containing at least a divinyl hydrocarbon and other components, the process including (a) passing a composition mixture feed stream containing at least (i) a divinyl hydrocarbon; and (ii) a monovinyl hydrocarbon, and/or (iii) a non-vinyl hydrocarbon through a process-scale chromatography unit; wherein the process scale chromatography unit includes a ligand exchange media comprising a metal adapted to form a ligand with an olefin functionality; wherein the metal of the ligand exchange media is loaded on an adsorbent; and wherein the divinyl hydrocarbon is adsorbed onto the ligand exchange media; (b) passing a weak first elution solvent through the unit to elute the monovinyl hydrocarbon and/or the non-vinyl hydrocarbon from the unit; (c) passing a strong second elution solvent through the unit to elute the divinyl hydrocarbon product stream from the unit; and (d) recovering the divinyl hydrocarbon product stream having been separated from the other components in the composition mixture stream; and an apparatus therefor.. .
Blue Cube Ip Llc


Mobile phase controller for supercritical fluid chromatography systems


The present disclosure relates to an apparatus for regulating the average mobile phase density or pressure in a carbon dioxide based separation system. The apparatus includes a controller, a set of pressure or density sensors and a set of instructions capable of determining the pressure drop across a column and adjusting at least one system component or parameter to achieve a pre-determined average mobile phase density or pressure in the system..
Waters Technologies Corporation


Automated sampling and reaction system for high pressure liquid chromatography and other types of detection


Automated sampling and reaction systems and methods of using the same are provided. The automated sampling and reaction system has a microreactor in fluidic communication with an external sampling valve.
Waters Technologies Corporation


Methods for determination of total homocysteine


Disclosed herein are simple, rapid and cost effective methods for determination of endogenous sulfhydryl amino acid species from a sample, example a sample in clinical practice. The method can include mixing of a biological sample with an internal standards which does not require pretreatment or derivatization and/or isotopic dilution.
Castle Medical, Llc


Method for chiral separation of methamphetamine and amphatamine enantiomers


Methods for routine high throughput analysis with accurate results to determine the concentration of d- and/or l-isomers of methamphetamine and/or amphetamine in biological samples is provided. In some examples, the method includes mixing of a biological sample with an internal standard and diluting with a mobile phase, followed by eluting on a chiral stationary phase contained in a liquid chromatography column.
Castle Medical, Llc


Modular automated chromatography system


Valves, pumps, detectors, sample loops, fraction collectors and the like are individually incorporated into modules that are mountable in interchangeable manner at individual mounting sites on a base unit which also supports one or more chromatography columns. Each module includes fluid connections to other modules and a microcontroller joining the module to a computed and monitor through an electronic connector at each mounting site.
Bio-rad Laboratories, Inc.


Chromatographic device and method


A chromatographic device for analysing multiple samples comprises a separator for separating the multiple samples, an injector unit for injecting the multiple samples into the separator, the injector unit being provided as regular injector unit for single-sample chromatography, a detector for detecting signals of the multiple samples after they are separated by the separator, a sequence generator for generating a specific binary sequence for each of the multiple samples, a controller that controls the injector unit so that each of the multiple samples is injected into the separator in pulses corresponding to its specific binary sequence, and a decomposer for decomposing the detected signals of the multiple samples by identifying the specific binary sequences in the detected signals.. .
Ruprecht-karts-universitat Heidelberg


Protein a chromatography


The present invention provides methods for cleaning a protein a chromatography column employing a media comprising a protein a ligand derived from the c domain of staphylococcus aureus, such that the column can be cleaned using both acidic and alkaline solutions.. .
Emd Millipore Corporation


Method for separating fat-soluble substance by simulated moving bed chromatography - and device for same


The present invention provides a separation method and device, comprising columns with at least two types of gels selected from the group consisting of silica gel, silver nitrate-impregnated gel and polymer gel filled therein, preferably at least three silver nitrate-impregnated gel columns and at least one silica gel column, by smb chromatography. The present invention also provides a separation method and device, comprising columns with at least two types of columns selected from the group consisting of c18 columns, c8 columns c4 columns and c1 columns, preferably at least three c18 columns and at least one c8 column and/or c4 column and/or c1 column, by smb chromatography..
Bizen Chemical Co., Ltd.


Protein products and methods for making the same


According to one embodiment, a protein product may include a mixture of water and particulate matter comprising protein. The mixture may include medium chain aldehydes and pyrazines.
Kraft Foods Group Brands Llc


Thin-layer chromatography type radiation detector


A thin-layer chromatography type radiation detector includes a plurality of light-emitting members configured to generate visible light due to radiation, each of the light-emitting members including a scintillator having a surface filled with a reflector for reflecting the visible light, and are arranged in parallel to one another in a width direction, light sensors respectively coupled to one end portions of the plurality of light-emitting members and configured to measure a current generated due to the visible light generated by the plurality of light-emitting members, and an output port configured to sequentially output values of the current measured by the light sensors as digital signals.. .

Proteomic biomarkers of sepsis in elderly patients


A proteomic expression platform to identify age-related sepsis risk is disclosed using patients with community-acquired pneumonia. A semi-quantitative plasma proteomics workflow was applied which incorporated tandem immuno affinity depletion, itraq labeling, strong cation exchange fractionation, and nanoflow-liquid chromatography coupled to high resolution mass spectrometry.

Method of amplifying detection light using light-reflecting material in immunochromatography


The present invention intends to provide an immunochromatographic test piece that makes it possible to achieve both highly sensitive detection of a substance to be detected and a simple test piece structure, which are usually difficult to be made compatible with each other. The immunochromatographic test piece is an immunochromatographic test piece comprising a membrane on which a capture substance being a ligand that bonds to a substance to be detected is immobilized, wherein insoluble carrier particles to which a ligand that bonds to the substance to be detected is bound are used and accumulated by being captured with the capture substance immobilized on the membrane, the membrane is irradiated with light to detect light emitted from a portion where the insoluble carrier particles are accumulated or light emitted from a portion surrounding and other than the portion where the insoluble carrier particles are accumulated, thereby measuring the substance to be detected, and a light-reflecting material is provided on a side of the membrane opposite to a side irradiated with light..

Method and system for detecting boron ions using ion chromatography for online monitoring of steam generator tube leakage in light water reactor


The present invention relates to an online leakage monitoring technique of a steam generator tube for monitoring leakage of the steam generator tube by analyzing concentration of an extremely small amount of boron ions in the secondary side solution of the steam generator in which a variety of ions are mixed, and the present invention is effective in that concentration of an extremely small amount of boron ions can be accurately detected, maintenance is convenient and durability is improved since analysis time is reduced considerably and operation pressure is lowered greatly by using ion chromatography provided with a boron trapping column optimized for trapping an extremely small amount of boron ions and a deionization water supplier for rising a sample line, instead of general ion chromatography provided with a concentration column and a separation column.. .

Apparatus and introducing sample into a separation unit of a chromatography system without disrupting a mobile phase


A method and a system for introducing a sample into a mobile phase of a chromatography system is provided. The method includes initially directing the mobile phase directly into a separation unit of the chromatography system, bypassing a sample loop, the mobile phase including a combined solvent, metered from a pressurized first solvent and a second solvent; loading the sample into the sample loop, while the mobile phase continues to be directed directly into the separation unit; pressurizing the sample in the sample loop with the pressurized first solvent, while the mobile phase continues to be directed directly into the separation unit; and switching the sample loop into the mobile phase, thereby introducing the pressurized sample to the separation unit..

Ethyleneimine polymer and producing the same


An ethyleneimine polymer, in which the number average molecular weight (mn) of the ethyleneimine polymer measured by gel permeation chromatography in terms of pullulan is 13,000 or more and the value obtained by dividing the weight average molecular weight by the number average molecular weight (weight average molecular weight: mw/number average molecular weight: mn=degree of dispersion) of the ethyleneimine polymer is from 1.4 to 3.0.. .

Purification of chimeric fviii molecules


The invention is directed to methods of purifying a chimeric protein comprising subjecting the chimeric protein to a factor viii-specific affinity chromatography, and subjecting the chimeric protein to an aex chromatography; wherein the chimeric protein comprises a factor viii protein or a fragment thereof. The chimeric protein purified by the present methods shows improved factor viii activity..

Solid phase for mixed-mode chromatographic purification of proteins


Proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of no more than three atoms between the hydrophobic group and the support matrix.. .

Fluid mixer assembly


Disclosed is an assembly for mixing fluids (i.e., gases or liquids), and more particularly an assembly that accurately mixes two or more high-pressure fluid sources and is adapted for use in applications, such as for example, chromatography. The mixer assembly (100) includes, inter alia, a housing (10), an inlet fitting (40), and a mixer cartridge assembly (60).

Chromatographic columns and separation devices comprising a superficially porous material; and use thereof for supercritical fluid chromatography and other chromatography


The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are superficially porous chromatographic particulate materials comprising sized less than 2 microns..

Means for generating cell-disintegrated blood


A method for analyzing a whole blood sample can include injecting whole blood into a first zone of a dual zone sample loop, applying sufficient heat or energy to the whole blood to disintegrate the cellular components of the whole blood sample to produce cell disintegrated blood, and injecting a sufficient volume of the buffer into the dual zone sample loop to move the cell disintegrated blood into a second zone of the dual zone sample loop. The method can further include switching a multiport value to an inject position, flowing the cell disintegrated blood from the dual zone sample loop into a solid phase extraction column, and eluting components of the cell disintegrated blood from the solid phase extraction column into a liquid chromatography column..

Fluid composition and reservoir analysis using gas chromatography


Methods are provided for reservoir analysis. In some embodiments, a reservoir may be analyzed by obtaining abundance ratios at a first measurement station and a second measurement station and determining an abundance ratio trend.

Fluid composition using optical analysis and gas chromatography


Methods and systems are provided for determining a gas/oil ratio using gas chromatography and optical analysis of a fluid sample obtained using a fluid sampling tool. In some embodiments, a gas/oil ratio may be determined from the mass fraction of each light component of the fluid, the mass fraction of each intermediate component of the fluid, a molecular weight of each light component of the fluid, a molecular weight of each intermediate component of the fluid, the density of stock tank oil, the vapor mass fraction of the intermediate components of the fluid, and the mass fraction of the plus fraction of the fluid.

Devices and methods for controlling reversible chemical reactions at solid-liquid interfaces by rapid preconcentration and phase replacement


Devices and methods for controlling reversible chemical reactions at solid-liquid interfaces are disclosed. In particular, the invention relates to a method of increasing reaction rates by concentrating a target molecule in a liquid phase in the region of a reactant or ligand immobilized on a solid followed by removal of the liquid phase and replacement with an immiscible phase, such as an immiscible gas or liquid to impede the reverse reaction.

Filter element and filtration assembly for biopharmaceutical applications


Provided are filter elements or cells and filtration assemblies or capsules for purifying and/or clarifying fluids used during various biopharmaceutical applications, for example, chromatography or depth filtration, and processes for making and using the same. A filter element comprises a media pack comprising two sides and a separator element located between the two sides, at least one side comprising a filtration media, wherein an inner edge periphery and/or an outer edge periphery of the filtration media comprise a bond directly to a portion of the separator element..

Aluminum chlorohydrate salts exhibiting high sec peak 3


An aluminum chlorohydrate salt having a peak 3:peak 4 ratio of at least 10:1 and an amount of peak 3 material relative based on a total of peaks 2, 3, 4, and 5 is at least 80% as measured by size exclusion chromatography, together with water treatment compositions, antiperspirant compositions, and oral care compositions, comprising the same, and methods for making and using the same.. .

Techniques for determination of haloacetic acid ("haa") presence in aqueous solution


A real-time method and analytical system for determining haloacetic acids in water which operate by: (1) extracting samples on ion-exchange absorbent medium; (2) concentrating haloacetic acids on hyper-crosslinked medium; (3) eluting the analytes from the concentration medium for injection into an hplc system; (4) separating individual haloacetic acid in reverse-phase chromatography performed by the hplc system; and (5) measuring optical characteristics (uv-absorbance) of haloacetic acids, to determine concentration. The entire process can be performed using a completely self-contained, in-situ mechanism that sits at a water distribution point for 24/7 testing, with automated control, monitoring, reporting, and employment of remedial measures (e.g., automated adjustment of the water treatment process)..

Pre-assembled separation columns


There is provided an integrated system for liquid separation, such as lc, ce, affinity chromatography, and ion exchange chromatography, comprising a column and end-fittings embedded in a plastic material, such as a thermoplastic polymer. The system may further comprise an electrospray emitter directly connected with the outlet of the column, wherein a substantial part of the emitter is covered with the polymer material.
Proxeon Biosystems A/s


Method for manufacturing a gas phase chromatography column and column obtained using such a method


The present invention also relates to a capillary column as well as to a microcolumn which may be manufactured according to this method.. .

Pressure sensing and flow control in diffusion-bonded planar devices for fluid chromatography


Flow through pressure sensors for use in fluid chromatography systems include a planar device formed from diffusion bonding of a plurality of metallic sheets and at least one sensing element. The planar device has a top surface, a bottom surface and a flow through channel.
Waters Technologies Corporation


Purification virus-like particles


The present invention aims at establishing a novel means for purifying norovirus vlps. There is provided a purification method of norovirus virus-like particles, comprising: contacting solution containing norovirus virus-like particles with support for hydroxyapatite chromatography, binding the virus-like particles to the support, subsequently washing the support with buffer, and then eluting the virus-like particles from the support with buffer containing phosphate, wherein the phosphate concentration of the buffer used for elution is less than 10 mm..
Umn Pharma Inc.


Affinity chromatography matrix


The invention relates to an affinity chromatography matrix, as a gel, comprising polymeric particles on which at least one oligosaccharide corresponding to a blood group a epitope and/or blood group b is grafted, via a spacer, characterized in that the density of oligosaccharides is comprised between 0.2 and 0.7 mg/ml of matrix. The invention also relates to the uses of this matrix for preparing concentrates of immunoglobulins for therapeutic use..
Laboratoire Francais Du Fractionnement Et Des Biotechnologies


Chromatography matrices including novel staphylococcus aureus protein a based ligands


The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, staphylococcus aureus protein a (spa), as well as methods of using the same.. .
Emd Millipore Corporation


Process for the preparation of high purity miglustat


A process for the preparation and isolation of crystalline miglustat without the use of a column chromatography or ion exchange purification. The crystalline miglustat has a high purity and a melting point of 128° c.

Preparative chromatography column and methods


A chromatography column that captures components in a process liquid in a free flow state and allows elution in steps is described.. .
Therapeutic Proteins International, Llc


Ev71 virus-like particles and preparation method and application thereof


The present invention provides ev71 virus-like particles and a preparation method and application thereof. The method comprises: connecting a p1 protein gene and a 3cd protease gene of an ev71 virus with a pmv plasmid to construct a pmv-p1-3cd recombinant expression plasmid; then transforming a hansenula polymorpha au-0501 expression strain with the pmv-p1-3cd recombinant expression plasmid to obtain an au-pmv-p1-3cd recombinant expression strain; fermenting and culturing the recombinant expression strain, and inducing the recombinant expression strain to express the ev71 virus-like particle protein with methanol; centrifuging and collecting mycelia for homogeneous breakage at a high pressure; and purifying the supernatant through ion-exchange chromatography, hydrophobic chromatography, and molecular sieve chromatography, so as to obtain ev71 virus-like particles..
Beijing Minhai Biotechnology Co., Ltd


Antibody-mediated anti-tumor activity induced by reishi mushroom polysaccharides


Immunogenic compositions, cancer vaccines and methods for treating cancer comprising fms, the fucose-enriched polysaccharide fraction from reishi f3, are provided. Compositions comprise fucose-enriched reishi polysaccharide fraction (fms) mw=˜35 kda, wherein the fms is isolated by size-exclusion chromatography from reishi f3, and the fms comprises polysaccharides having primarily a backbone selected from 1,4-mannan and 1,6-α-galactan, wherein the backbone is linked to a terminal fucose-containing side-chain immunogenic compositions comprising glycolipid adjuvants are provided.
Academia Sinica


Elucidation of ion exchange chromatography input optimization


The present invention provides methods for determining chromatography separation conditions; for example, separation of a polypeptide and its charge variants. The invention also provides methods to determine a buffer condition for chromatography separation conditions.
Genentech, Inc.


High resolution analyte recovery system and method


Analyte separation systems for use in conjunction with chromatography separation devices and techniques are described. Disclosed systems include modular components that can provide for individualized design of separation schemes to better fit the needs of the user.
Clemson University


Stackable planar adsorptive devices


Adsorptive bed devices include a scaffold in housing having a stress absorbing rigid structure and open cells filled with adsorptive beads. The scaffold restricts movement of the plurality of adsorptive beads, absorbs stress induced by a hydraulic pressure gradient along a direction of liquid flow and transfers a portion of the induced compressive stress to the housing.
Spf Technologies Llc


Backflush methods and devices for chromatography


Certain embodiments described herein are directed to chromatography systems that include a microfluidic device and that implement one or more methods to direct sample to a desired fluid flow path. The methods can be used to backflush a sample to a desired fluid flow path to select certain analytes within a sample..

Purification of long chain diacids


The present disclosure relates to methods for separating and purifying a long chain diacid from other long chain diacids, monocarboxylic acids, hydroxyl acids or alkanes by simulated or actual moving bed chromatography.. .
Invista North America S.á.r.l.


Modified diamond particles


Modified diamond particles for use in chromatography with a desired functional group at the diamond surface, formed from reaction of hydroxyl groups at diamond surfaces with a reactive molecule.. .
Brigham Young University


Ring shaped counter electrode to improve beam stability and compound sensitivity on a ceramic tile type microfluidic device


An interface for a mass spectrometer is disclosed comprising a microfluidic substrate, tile or cartridge 1 comprising a liquid chromatography separation column and an electrospray emitter 2. A counter electrode 4 is arranged downstream of a tip of the electrospray emitter 2 and is arranged and adapted to direct ions towards an atmospheric pressure interface or ion inlet aperture 5 of a mass spectrometer..
Micromass Uk Limited


Monolithic column chromatography


Provided herein are methods of liquid column chromatography in which preparative chromatography is performed in-line with analytical chromatography. In particular aspects a monolithic preparative column is used to purify an analyte of interest from a mixture of other substances by applying the mixture to the column, reversing the flow through the column to elute the analyte, which is applied to an analytical column provided in-line with the preparative column.
Quest Diagnostics Investments Incorporated


Gradient liquid feed device for sample analyzer


A gradient liquid feed device allows reduction in size of a high performance liquid chromatography type sample analyzer. The gradient liquid feed device includes a plurality of carrier liquid reservoir tanks configured to store carrier liquids of mutually different compositions, a plurality of single plunger pumps capable of drawing and discharging the carrier liquid from the plurality of carrier liquid reservoir tanks, a mixer configured to mix the carrier liquids discharged from the plurality of liquid feed pumps and feed the mixed carrier liquid, and a pulse damper in communication with the mixer and configured to absorb pulsation that may occur during liquid feeding.
Sekisui Medical Co., Ltd.


Liquid chromatography fast measuring


Provided is a liquid chromatography apparatus for fast measuring, and more particularly, a liquid chromatography apparatus which includes a first column and a second column having different component separations for an interest component within a sample and makes components primarily separated in the first column be secondarily separated in the second column of a first channel unit before a switching valve is switched, makes the components primarily separated in the first column be continuously discharged through a second channel unit after the switching valve is switched, thereby improving the component separation and achieving the fast measuring.. .
Whitech Inc.


Inspection tool for nucleic acid chromatography


An inspection tool for nucleic acid chromatography includes an elongated porous sheet and a backing member. The porous sheet has a surface including a detection surface that has a strip-shaped indication portion where a nucleic acid probe for capturing the target nucleic acid is fixed.
Ngk Insulators, Ltd.


Process for increased yield of immunoglobulin from human plasma


The present invention discloses a preparation method for large scale production of human immunoglobulins (igg) with high yields by an improved all-chromatography process scheme that eliminates ethanol precipitation. The process of extracting immunoglobulins is such that the other therapeutic proteins in plasma are left unaffected and are available for extraction separately from the same plasma sample.
Hemarus Therapeutics Limited


Methods for the detection of spoilage of oils


Methods for the detection of spoilage of oils containing unsaturated fatty acids is disclosed. Specific methods include the determination of p-anisidine in oils containing unsaturated fatty acids using high performance liquid chromatography after p-an-isidine is added to the oil containing unsaturated fatty acids, and allowed to react with oxidation products..
Nordic Naturals, Inc.


Method of detecting and quantifying perchlorate contamination


A method of detecting and quantifying perchlorate contamination in samples, especially samples with complex background matrices such as food and produce with trace levels of perchlorate. A simple microwave assisted-electromembrane extraction provides simultaneous reduced extraction time, sample clean-up, high recovery and enrichment of perchlorate ions for detection and quantification by ion chromatography.
King Fahd University Of Petroleum And Minerals




Chromatography topics:
  • Chromatography
  • Chromatograph
  • Antibodies
  • Mass Spectrometry
  • Spectrometry
  • Quantitative
  • High Resolution
  • Switching Valve
  • Cross Section
  • Protein A Affinity Chromatography
  • Amino Acid
  • Silica Gel
  • Carboxylic Acid
  • Antifungal
  • Isoleucine


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