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Chromatography patents



      
           
This page is updated frequently with new Chromatography-related patent applications. Subscribe to the Chromatography RSS feed to automatically get the update: related Chromatography RSS feeds. RSS updates for this page: Chromatography RSS RSS


Date/App# patent app List of recent Chromatography-related patents
10/23/14
20140316114
 Method for preparing active form of tnfr-fc fusion protein patent thumbnailMethod for preparing active form of tnfr-fc fusion protein
The present invention relates to a method for separating and preparing a tnfr-fc fusion protein using hydrophobic interaction chromatography (hic). More particularly, the present invention relates to a method for separating and preparing a highly pure active protein from clipped proteins due to displacement effect by adjusting the conductivity of a protein sample using a high concentration of salt solution and by adjusting a loading amount thereof, and a tnfr-fc fusion protein prepared by the method..
10/23/14
20140316108
 Protein chromatography matrices with hydrophilic copolymer coatings patent thumbnailProtein chromatography matrices with hydrophilic copolymer coatings
A coating of a random copolymer of acrylamide and a second monomer, e.g. Glycidoxylmethacrylate, for a silica surface is described.
10/23/14
20140315322
 Method for measuring acetic acid concentration in blood plasma patent thumbnailMethod for measuring acetic acid concentration in blood plasma
The present invention relates to providing a simple and highly reproducible method for measuring the concentration of acetic acid in blood plasma by using a gas chromatography/mass spectrometry (gc/ms), and more specifically relates to a method for measuring the concentration of acetic acid in blood plasma by using a gas chromatography/mass spectrometry (gc/ms), which comprises extracting acetic acid in blood plasma with methyl-tert-butyl ether (mtbe).. .
10/23/14
20140315318
 Analytical methods for analyzing and determining impurities in dianhydrogalactitol patent thumbnailAnalytical methods for analyzing and determining impurities in dianhydrogalactitol
An improved analytical method for analysis of dianhydrogalactitol preparations provides a method for determining the purity of dianhydrogalactitol and detecting impurities in preparations of dianhydrogalactitol, as well as identifying any such impurities. The method employs high performance liquid chromatography (hplc), in particular, hplc with evaporative light scattering detection (elsd); the hplc can be followed by tandem mass spectroscopy.
10/23/14
20140315231
 Quantification of transthyretin and its isoforms patent thumbnailQuantification of transthyretin and its isoforms
The present invention relates to assays and methods for the detection of transthyretin and its isoforms. Specifically, the assays and methods of the present invention embrace liquid chromatography and mass spectrometry.
10/23/14
20140311977
 Use of organic solvent nanofiltration and liquid-liquid chromatography for the recovery of pharmaceutical products patent thumbnailUse of organic solvent nanofiltration and liquid-liquid chromatography for the recovery of pharmaceutical products
A process for separating a compound from solution in organic solvent by a chromatographic process, in particular liquid-liquid chromatography, in which prior to the chromatographic process the composition of the solution is changed by means of a process of organic solvent nanofiltration for a solvent exchange. Additionally or alternatively subsequent to the chromatographic process the output from the chromatographic process is subjected to a process of organic solvent nanofiltration to remove residual target compound and/or impurities in the output for solvent recovery..
10/16/14
20140306105
 Liquid chromatrography systems and methods patent thumbnailLiquid chromatrography systems and methods
One aspect of the invention provides a liquid chromatography system including: a first solvent manager configured to dispense various ratios of a first solvent and a second solvent; a first column in fluid communication with the first solvent manager; a mixer in fluid communication with the first column; a first valve in fluid communication with the mixer; a second column having a first end in fluid communication with a first port of the first valve and a second end in fluid communication with a second port of the first valve; a second solvent manager adapted and configured to dispense various ratios of a third solvent and a fourth solvent; and a second valve in fluid communication with the second solvent manager, the first valve, and the mixer. The first valve and the second valve are adapted and configured for actuation between and a second position.
10/16/14
20140305195
 Preparative column chromatography system patent thumbnailPreparative column chromatography system
A chromatography system comprising a mixing circuit or a mixing chamber, a bubble trap, a concentration detector and one or more pumps, characterized in that the bubble trap has a permanent opening at its highest point is described herein. Furthermore, a chromatography system is described, characterized in that it contains two concentration detectors the first of which is located in the mixing circuit or the mixing chamber and the second is located downstream of the main pump..
10/09/14
20140303060
 Process for degrading a biofilm on surfaces of objects patent thumbnailProcess for degrading a biofilm on surfaces of objects
Optionally followed by further purification steps, such as ion-exchange chromatography and/or concentration.. .
10/09/14
20140303014
 Multiplexed detection with isotope-coded reporters patent thumbnailMultiplexed detection with isotope-coded reporters
Some aspects of this invention provide reagents and methods for the sensitive, quantitative and simultaneous detection of target analytes in complex biological samples by liquid chromatography tandem mass spectrometry (lc ms/ms). Some aspects of this invention provide affinity reagents encoded with mass reporters for the sensitive and quantitative translation of an analyte of interest into a mass tag.
10/09/14
20140302592
Protein purification by anion exchange chromatography
The present invention relates to a two-step method for the purification of divalent cation binding proteins with high yield and high purity on anion exchange resin materials, to divalent cation binding proteins obtainable by said method, and to a kit comprising means for carrying out said method.. .
10/09/14
20140302591
Protein purification by anion exchange chromatography
The present invention relates to a two-step method for the purification of divalent cation binding proteins with high yield and high purity on anion exchange resin materials, to divalent cation binding proteins obtainable by said method, and to a kit comprising means for carrying out said method.. .
10/09/14
20140301977
Overload and elute chromatography
The present invention provides methods for purifying a polypeptide from a composition comprising the polypeptide and at least one contaminant by overloading a chromatography material and eluting the product.. .
10/09/14
20140301757
Toner, developer using the toner, image forming apparatus
A toner including at least a crystalline resin as a binder resin, wherein a tetrahydrofuran soluble content of the toner includes 5.0% or more as a peak area of a component having a molecular weight of 100,000 or greater in a molecular weight distribution measured by gel permeation chromatography, and the tetrahydrofuran soluble content of the toner has a weight-average molecular weight of 20,000 to 60,000.. .
10/09/14
20140299765
Apparatus for improved immunosuppressant drug monitoring
A liquid chromatography/mass spectrometry system includes: a source of a first mobile phase solvent consisting essentially of water plus 10 mm ammonium formate plus 0.05% formic acid; a source of a second mobile phase solvent consisting essentially of methanol plus 10 mm ammonium formate plus 0.05% formic acid; a chromatography column comprising a length of 30 mm or less of a stationary phase comprising an 8-carbon alkyl chain material bonded to 2.6 μm diameter particles having solid silica cores surrounded by porous silica outer layers; an electrospray ion source of a mass spectrometer fluidically coupled to the chromatography column so as to generate ions therefrom; a mass analyzer of the mass spectrometer operable to quantitatively detect the ions; and a programmable processor electronically coupled to the mass analyzer and comprising instructions operable to determine, based on the ion detection, a concentration of everolimus, sirolimus, tacrolimus, or cyclosporin a.. .
10/09/14
20140299542
Method and system for liquid chromatography fluidic monitoring
A method for monitoring a fluidic system of a liquid chromatography system is characterized by: (a) drawing a fluid into a syringe pump; (b) configuring a valve so as to fluidically couple the pump to either a fluidic pathway through the fluidic system or to a plug that prevents fluid flow; (c) causing the syringe pump to progressively compress the fluid therein or expel the fluid to the fluidic pathway, while measuring a pressure of the fluid; (d) determining a profile of the variation of the measured pressure; (e) comparing the determined profile to an expected profile that depends upon the fluid; and (f) providing a notification of a sub-optimal operating condition or malfunction if the determined profile varies from the expected profile by greater than a predetermined tolerance.. .
10/09/14
20140299510
Slurry oil upgrading while preserving aromatic content
Methods relating to methods for producing a needle coke precursor from slurry oil having low levels of nitrogen and sulfur. Nitrogen-containing compounds are removed by chromatography, followed by hydrotreating at relatively mild conditions that focus on the more easily-removed sulfur-containing compounds while largely preserving aromatic content of the slurry oil.
10/09/14
20140298990
Devices and methods for adaptive micro-gas chromatography
The present disclosure provides adaptive methods for gas chromatography analysis of a gas sample comprising one or more target analytes (such as a micro-gas chromatography) and adaptive gas chromatography devices for carrying out such analytical methods. Broadly, the system can regulate flow into a downstream chromatographic column by detecting one or more upstream conditions.
10/09/14
20140298924
Chromatography apparatus and method
Sample preparation device for receiving in sealing relation one or more filter assemblies and one or more sample containers, each container uniquely positioned within the device to receive filtered sample from a designated filter. The device includes a body, a lid disposed on the body, a container tray, a base, an integrated seal on the body, a valve and a valve actuator.
10/02/14
20140296500
Methods and compositions of nucleic acid ligands for detection of clinical analytes related to human health
Specific dna sequences for binding various clinically relevant analytes from the human body are described. Each of these sequences or their linear, two- and three-dimensional linked sequences can function in varying assay and sensor formats with varying degrees of success.
10/02/14
20140296485
Anionic displacer molecules for hydrophobic displacement chromatography
A process for separating organic compounds from a mixture by reverse-phase displacement chromatography, including providing a hydrophobic stationary phase; applying to the hydrophobic stationary phase a mixture comprising organic compounds to be separated; displacing the organic compounds from the hydrophobic stationary phase by applying thereto an aqueous composition comprising a non-surface active hydrophobic anionic displacer molecule and about 10 wt % or less of an organic solvent; and collecting a plurality of fractions eluted from the hydrophobic stationary phase containing the separated organic compounds; in which the non-surface active hydrophobic anionic displacer molecule comprises a hydrophobic anion and a counterion, ci, having the general formula a or b, as defined in the disclosure: [cm][cl]d [cm-r*—cm′][cl]d a b.. .
10/02/14
20140296464
Chromatography medium
The present invention provides a chromatography medium comprising one or more electrospun polymer nanofibres which form a stationary phase comprising a plurality of pores through which a mobile phase can permeate and use of the same in chromatography, such as the isolation of recombinant proteins, monoclonal antibodies, viral vaccines and plasmid dna. The invention further provides for the use of the chromatographic medium in a simulated moving bed system..
10/02/14
20140296434
Chromatography matrices including novel staphylococcus aureus protein a based ligands
The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, staphylococcus aureus protein a (spa), as well as methods of using the same.. .
10/02/14
20140295405
Chromatography method, and chromatography kit
The chromatography method of the present invention includes (a) developing a complex of a test substance and a labeling substance modified with a first binding substance bindable to the test substance, onto an insoluble carrier, (b) causing the complex to be trapped in a reaction site on the insoluble carrier which contains a second binding substance bindable to the test substance or a substance exhibiting binding properties to the first binding substance, (c) washing the insoluble carrier with a washing solution containing at least one of the potassium iodide, urea, and guanidine after the step (b), (d) washing off the washing solution of the step (c) remaining in the insoluble carrier from the insoluble carrier, and (e) amplifying the labeling substance of the complex trapped in the reaction site.. .
10/02/14
20140294745
Coral extract for skin whitening, moisture retention, elasticity improvement, anti-inflammation and occlusion of wounds and extracting method thereof
A coral extract for skin whitening, moisture retention, elasticity improvement, anti-inflammation and occlusion of wounds and its extracting method is provided. The coral extract is extracted from briareum excavatum.
10/02/14
20140290645
Methods and controllers for simulated moving bed chromatography for multicomponent separation
A system for reacting and separating solid and fluid components includes a series of sequential simulated moving bed (smb) chromatography columns connected to form a circulation loop. Each smb chromatography column includes an inlet and an outlet.
10/02/14
20140290491
Column unit and gas chromatography device provided with the same
A housing of a column unit is tubular-shaped, and includes a first opening and a second opening, and an inner space separated from outside air by an insulating material. An air cooling section including a cooling device is arranged on a side of the first opening of the housing.
09/25/14
20140288278
Chromatography process for resolving heterogeneous antibody aggregates
Disclosed are methods and processes utilizing multi-modal chromatography as a polishing step to separate heterogeneously charged (basic and acidic) aggregates and other impurities from partially a partially purified bulk product monoclonal antibody product. The resulting chromatographic process provides a scaleable production process that is cost effective and increases the productivity of the purification process over a platform utilizing a combination of anion and cation exchange chromatography to resolve heterogeneous aggregates..
09/25/14
20140287146
Production process of film and column for cation chromatography
One object of the present invention is to produce a weakly acidic cation exchanger under mild conditions. Another object of the present invention is to produce a more firm weakly acidic cation exchange film.
09/25/14
20140284472
Ion mobility spectrometer
A method and apparatus are disclosed for improving ion mobility spectrometry by using a fast and spatially wide ion gate based on local rf field barrier opposed to a switching dc field. The improvement accelerates the ion mobility analysis and improves charge throughput and dynamic range of the ims.
09/25/14
20140284279
Apparatus, system and method for mass directed chromatography
The present invention provides an apparatus that can be used to connect a flash chromatography instrument to a fraction collector and a mass detector to perform mass-directed flash chromatography.. .
09/25/14
20140284278
Cationic displacer molecules for hydrophobic displacement chromatography
A process for separating organic compounds from a mixture by reverse-phase displacement chromatography, including providing a hydrophobic stationary phase; applying to the hydrophobic stationary phase a mixture comprising organic compounds to be separated; displacing the organic compounds from the hydrophobic stationary phase by applying thereto an aqueous composition comprising a non-surface active hydrophobic cationic displacer molecule and about 10 wt % or less of an organic solvent; and collecting a plurality of fractions eluted from the hydrophobic stationary phase containing the separated organic compounds; in which the non-surface active hydrophobic cationic displacer molecule comprises a hydrophobic cation and a counterion, ci, having the general formula a or b, as defined in the disclosure:. .
09/25/14
20140284277
Processing biomass materials
Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, equipment, systems and methods are described that can be used to treat feedstock materials, such as cellulosic and/or lignocellulosic materials.
09/25/14
20140284270
Rna pores and methods and compositions for making and using same
Synthetic rna molecules having a pore, compositions including rna molecules having a pore, where the compositions are capable of filtering metal ions, filtering molecular ions, performing size exclusion chromatography, performing ion specific chromatography, reversible metal ion and molecular ion binding, ion selective membranes, ion selective channels, ion selective/specific sensors, electrical conduits, battery components, etc., and devices and methods for making and using same. The rnas used may be modified to improve stability.
09/25/14
20140284260
Chromatography column
A chromatography column having a longitudinal axis and comprising a column wall with a first end and a second end, a first end plate assembly removably connectable to said first end of the column wall, a second end plate assembly removably connectable to said second end of the column wall, wherein said first end plate assembly, said column wall and said second end plate assembly are arranged along the longitudinal axis of the column wherein the column wall, and/or first end plate assembly and/or second end plate assembly is/are rotatable about an axis of rotation wherein said axis of rotation is parallel to the longitudinal axis of said column and positioned outside the column. .
09/25/14
20140283688
Self cleaning gas-liquid separator for serial or parallel collection of liquid fractions
An apparatus, process, and system are disclosed that effectively provide separation of a combined gas/liquid flow stream into its separated gas and liquid factions. The invention is primarily directed to the fields of preparative supercritical fluid chromatography [sfc] and supercritical fluid extraction [sfe], but will have other utilization and applicability where phases of dramatically different density, viscosity and volumetric flow require separation..
09/18/14
20140275594
New process for reducing pollutants in fats and oils and their derivatives
The present invention describes a process to reduce or eliminate at least one pollutant in a starting material, said starting material is at least one of an oil, a fat, its derivatives and mixtures thereof, of animal, krill, algae or microbial origin or biofuel, being totally or partially esterified or transesterified, or of vegetable origin, being raw, esterified or transesterified, to which optionally it is added a fluid, said fluid is at least one of an ester, a partial glyceride, a monoglyceride, a diglyceride and mixtures thereof, subjecting the starting material to at least one stage of extraction or chromatography or distillation. The process of the invention provides a product suitable to be used in food, pharmacy, cosmetic and as a dietary supplement..
09/18/14
20140275494
Protein purification using displacement chromatography
Disclosed herein are compositions and methods for the isolation and purification of proteins from a sample. In particular, the present invention relates to compositions and methods for isolating and purifying proteins incorporating a displacement chromatographic step.
09/18/14
20140275438
Bulk hydrophilic functionalization of polyamide 46
A modified polymer as result of a bulk functionalization of polyamide 46 (pa 46) is presented, as well as methods for synthesizing the modified polymer. This functionalization of pa 46 is performed to provide a homogenous semi-permeable polyamide 46 capable of different charges and different porosities with particles of nanoscale size in order to replace or improve other polyamide fibers used in the textile industry, filtering processes, selective sorption, controlled release devices, phase transfer catalysts, chromatography media, biocompatible capsules, artificial skins, organs, bone void repair as well as in cell bioreactors and incubators, dental implements, medical devices, clothing, detectors, perfusion devices, in regenerative medicine, and fuel cells..
09/18/14
20140274782
Affinity reagents and methods for detection, purification, and proteomic analysis of methylated proteins
Methyl-lysine affinity reagents created by engineering the 3×mbt methyl-lysine binding domain repeat of lethal (3) malignant brain tumor-like protein 1 (l3mbtl1) are disclosed. In particular, the invention relates to affinity reagents and affinity chromatography media comprising the 3×mbt domain repeat and methods of using such affinity reagents in detection, purification, and proteomic profiling of methylated proteins and peptides..
09/18/14
20140274751
Chemical identification using a chromatography retention index
Provided herein is technology relating to identifying unknown compounds and particularly, but not exclusively, to methods and systems for identifying unknown compounds by gas chromatography-mass spectrometry by use of retention index as a second dimension for identification.. .
09/18/14
20140273271
Fluorescence immuno-chromatography, kit and test strip for the same
Detecting another fluorescence components not exceeding the critical angle, the another fluorescence components being included in the fluorescence emitted from the fluorescent substance, the detection being made by virtue of the fluorescence components passing through the film.. .
09/18/14
20140273264
Epoxy chemistry derived materials as mixed mode chromatography media, method for their synthesis and use
This invention provides mixed-mode stationary phase compositions, devices and systems comprising the stationary phases as well as methods of producing these compositions using epoxide ring-opening reactions. Also provided are methods of using the stationary phases of the invention in separations..
09/18/14
20140273158
Chromatography membranes stable under caustic conditions
Disclosed are composite materials and methods of using them for chromatography. The composite materials retain their performance characteristics, such as binding capacity, flux, or percent recovery, under caustic conditions (e.g., 1 m naoh for 24 h).
09/18/14
20140268154
Chromatography system, signal processing apparatus, chromatography data processing apparatus, and program
A chromatography system has a multi-channel detection device including a flow cell, optics for directing light from light sources to the flow cell and outputting light that has passed through the flow cell, and a multi-channel detector. The optics has a function of dispersing light in wavelength in an optical path.
09/18/14
20140264003
Method for cleaning an atmospheric pressure chemical ionization source
Build-up of surface contamination within an atmospheric pressure chemical ionization (apci) source of a mass spectrometer (ms) is reversed by switching the apci polarity to the opposite setting used for analyte ionization after the analytes have been ionized and measured. A solvent or mixture of solvents is passed through the ion source during the opposite-polarity operation.
09/18/14
20140263012
Parallel assembly of chromatograpy column modules
A parallel assembly (2; 11; 51) of chromatography column modules (3a,b,c; 13a,b,c; 53a,b,c, 90a, b), the assembly having one common assembly inlet (15; 55) and one common assembly outlet (17; 57), each column module comprising a bed space (29) filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space (29) of the column module with the assembly inlet (15; 55) and the assembly outlet (17; 57), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.. .
09/18/14
20140261868
Bottle pressurization delivery system
A container assembly for use with a high-pressure liquid chromatography (hplc) instrument is disclosed, in which the container assembly, when coupled to a source of pressurized gas, provides fluid medium to the hplc instrument at positive pressure. The container assembly has an external exterior container shell, an internal fluid container for holding fluid medium, an interstitial volume between the external exterior container shell and the internal fluid container, a port for fluidly connecting the volume to a pressurized gas source, and a port for fluidly connecting the internal fluid container to the hplc instrument.
09/18/14
20140261397
Method of separating carbohydrate
Disclosed is a method of separating carbohydrate, including: mixing formic acid with heteropoly acid, chloride or bromide of lithium, magnesium, calcium, zinc, or iron, or combinations thereof to form a mixing liquid. The method also includes dissolving a cellulose biomass by the mixing liquid to form a solution, mixing water and the solution to hydrolyze the cellulose biomass for forming a carbohydrate solution, and mixing an extractant and the carbohydrate solution to extract the formic acid out of the carbohydrate solution.
09/18/14
20140260563
Epoxy chemistry derived materials as reversed-phase and hydrophobic interaction chromatography media, method for their synthesis and use
This invention provides aqueous-compatible, polar-embedded reversed-phase stationary phase compositions, devices and systems comprising the stationary phases as well as methods of producing these compositions using epoxide ring-opening reactions. Also provided are methods of using the stationary phases of the invention in separations..
09/18/14
20140260540
Sample inlet with multi-capillary liner for gas chromatography
A gas chromatograph (gc) inlet device includes a multi-capillary liner capable of separating components of a sample matrix prior to injecting the sample into an analytical gc column. The device is switchable between different modes, such as a normal injection modes, splitless modes, split modes, a backflush modes, and cut modes..
09/18/14
20140260535
Gas chromatographic "in column" spectroscopic analysis
A chemical detector for rapid, simultaneous detection of multiple chemicals including chemical warfare agents, toxic industrial chemicals, and explosives having one or more gas chromatography columns each with a chemosorbent or a chemo-reactive stationary phase and an infrared-transparent base, a bright infrared light source, a mechanism to direct the light source to any point along any of the columns, and an infrared sensor. Another disclosed detector has one or more gas chromatography columns each on the surface of a substrate having at least one infrared-transparent waveguide pattern, a bright infrared light source, and at least one ring resonator for each column, where each ring resonator is coated with a chemosorbent or a chemo-reactive stationary phase, and where each ring resonator spectroscopically probes the stationary phase.
09/18/14
20140260534
Biocompatible filter
An improved biocompatible filter for use in liquid chromatography systems such as hplc or uhplc the present disclosure addresses the problem of particles bypassing a filter in a liquid chromatography application by design features in the interface between the frit filter and ring that form an improved seal between the ring and frit and prevent leakage around the filter in high measure chromatography. Design features also include frit filters with an internal particle size gradient for pre-column separation activity during filtration..
09/18/14
20140260509
Method of calibrating a chromatography system
A method of calibrating, a chromatography system is described. The method includes injecting a standard into a chromatographic separator.
09/18/14
20140259616
Method for producing a fluidic connection component for chromatography
A method for producing a fluidic connection component for chromatography is described. A connection component includes a main body and at least one insert held in the main body.
09/11/14
20140256910
Neutral zwitterionic displacer molecules for hydrophobic displacement chromatography
A process for separating organic compounds from a mixture by reverse-phase displacement chromatography, including providing a hydrophobic stationary phase; applying to the hydrophobic stationary phase a mixture comprising organic compounds to be separated; displacing the organic compounds from the hydrophobic stationary phase by applying thereto an aqueous composition comprising a non-surface active hydrophobic neutral zwitterionic displacer molecule and optionally an organic solvent; and collecting a plurality of fractions eluted from the hydrophobic stationary phase containing the separated organic compounds; in which the non-surface active hydrophobic neutral zwitterionic displacer molecule comprises a hydrophobic zwitterion having the general formula, as defined in the disclosure: [cm-r*—cm′].. .
09/11/14
20140256899
Polymer films having improved heat sealing properties
A polymer composition comprising an ethylene alpha-olefin copolymer, wherein the polymer composition is characterized as having (a) a density in the range of from greater than about 0.910 g/cc to about 0.930 g/cc, as determined according to astm d1505; (b) a melt index in the range of from greater than about 0.5 g/10 min to about 3 g/10 min, as determined by astm d1238, condition 190° c./2.16 kg; (c) a molecular weight distribution of from about 3.4 to about 12, as determined by gel permeation chromatography (gpc); (d) a weight average molecular weight of from greater than about 85 kg/mol to about 160 kg/mol, as determined by gel permeation chromatography (gpc); and (e) a z-average molecular weight of from greater than about 210 kg/mol to about 500 kg/mol, as determined by gel permeation chromatography (gpc).. .
09/11/14
20140256673
Herbal extract composition for the treatment of diabetes and a method of extracting the same
The embodiments herein provide a method for isolation and purification of a novel oligosaccharide molecule from the fruits of rosa arvensis for the treatment of diabetes. The fruit is dried, powdered and subjected to deionized water.
09/11/14
20140255964
Analytical method of post-translational modifications in hemoglobin
An analytical method of post-translational modifications in hemoglobin is disclosed. The analytical method comprises the steps of providing a blood comprising the hemoglobin with post-translational modification of nitration, nitrosylation, or oxidation; performing an extraction process to the blood by an organic solvent; quantifying the hemoglobin by a fluorescent spectrometry; hydrolyzing the hemoglobin into a plurality of peptides by an enzyme; and using a nanoflow liquid chromatography-nano spray ionization tandem mass spectrometry to characterize and quantify the post-translational modifications of the hemoglobin..
09/11/14
20140255423
Isolation and purification of antibodies using protein a affinity chromatography
Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise ph viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography, preferably protein a affinity, ion exchange chromatography, and hydrophobic chromatography.


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Chromatography topics: Chromatography, Chromatograph, Antibodies, Mass Spectrometry, Spectrometry, Quantitative, High Resolution, Switching Valve, Cross Section, Protein A Affinity Chromatography, Amino Acid, Silica Gel, Carboxylic Acid, Antifungal, Isoleucine

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