|| List of recent Chromatography-related patents
| Automated system and method for monitoring chromatography column performance, and applications thereof|
The present invention provides automated systems and methods for monitoring column performance in process chromatography, and applications thereof. In an embodiment, column performance is monitored by generating a plurality of process values such as, for example, conductivity values or ph values with a detector during a chromatography step transition between a first mobile phase liquid and a second mobile phase liquid.
| Synthesis method of glyco-drug radiotracer precursor|
A novel synthesis method of glyco-drug radiotracer precursor is revealed. After completing synthesis of z-gly-ah (main structure), galactosamine galnac(oac)4 is added to have coupling reaction.
| Purification of biological conjugates by size exclusion chromatography|
(h) recovering the biological conjugate from the column.. .
| Multi-dimensional chromatographic methods for separating n-glycans|
A multi-dimensional chromatographic method for the separation of n-glycans. The method comprises providing a glycan preparation that includes at least one negatively charged n-glycan.
| Separatome-based protein expression and purification platform|
Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of e. Coli, yeast, bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc.
| Systems and methods for characterization of molecules|
The present invention generally provides systems and methods for the detection, identification, or characterization of differences between properties or behavior of corresponding species in two or more mixtures comprised of molecules, including biomolecules and/or molecules able to interact with biomolecules, using techniques such as partitioning. The experimental conditions established as distinguishing between the mixtures of the molecules using the systems and methods of the invention can also be used, in some cases, for further fractionation and/or characterization of the biomolecules and/or other molecules, using techniques such as single-step or multiple-step extraction, and/or by liquid-liquid partition chromatography.
| Method and apparatus for split-flow-mixing liquid chromatography|
A method for chromatographically separating analytes of a liquid sample comprises: (i) providing the sample in a conduit; (ii) providing a solvent for the sample; (iii) causing the solvent to simultaneously flow into the conduit so as to expel the sample from the conduit and flow into and through a second conduit so as to exit said second conduit; (iv) simultaneously providing the expelled sample and the exited solvent to a mixing tee-junction such that the expelled sample and the exited solvent mix thereat; (v) providing the mixture of the expelled sample and the exited solvent to a chromatographic column such that the analytes are transferred to the column and are chromatographically separated therein under the influence of a flow of the solvent, or a different solvent or a mixture of solvents.. .
| Mobile phase preparation device for liquid chromatography|
A mobile phase delivery device for use with high pressure liquid chromatography includes a manifold having a plurality of inlets, wherein each inlet is fluidly coupled with a solvent source. The manifold further has a mobile phase outlet that outputs a mobile phase, composed of solvent or solvent mixture, onto an analytical line.
|Method of producing and purifying an active soluble sialyltransferase|
The present invention relates to a method for the production and purification of a sialyltransferase polypeptide, in particular a n-acetylgalactosamine (gal nac)-α-2,6-sialyltransferase i (st6galnaci) polypeptide. The method comprises the steps of producing the sialyltransferase polypeptide in a chinese hamster ovary (cho) cell and purifying the polypeptide with a combination of chromatography steps.
|Pulsed discharge helium ionization detector with multiple combined bias/collecting electrodes for gas chromatography and method of use|
A pulsed discharge helium ionization detector for gas chromatography with multiple combined bias/collecting electrodes.. .
|Method of purification of prostaglandins including fluorine atoms by preparative hplc|
The present invention discloses a method of purification of prostaglandins including fluorine atoms by using preparative hplc. Tafluprost and travoprost are prostaglandins including fluorine.
|Process and methods for efficient manufacturing of highly pure asymmetric antibodies in mammalian cells|
The present invention provides a process and methods for producing asymmetric antibodies in a mammalian expression system. The asymmetric antibodies are transiently or stably expressed and in cells that stably express the asymmetric antibody, following a rapid 2-step process of stable pool to clone, a highly pure asymmetric antibody expressing clone can be identified at a success frequency that permits for screening of tens of clones rather than thousands.
|Instrument and method for analysis of mannose 6-phosphate|
Disclosed are an apparatus and method for separation analysis of mannose-6-phosphate (m6p) by post-column fluorescence detection method. The apparatus is based on chromatography, and includes a column with a solid phase having affinity for phosphate, a flow path for the eluate, a heater installed on the flow path for m6p and a basic amino acid to react by heating the eluate in the flow path, and a fluorescence detector installed downstream of the heater for continuously irradiating the eluate with excitation light and measuring the intensity of the emission, and may include in the flow path a supply channel for addition of a basic amino acid between the column and the heater.
|Integrated chromatography devices and systems for monitoring analytes in real time and methods for manufacturing the same|
Systems and methods for monitoring analytes in real time using integrated chromatography systems and devices. Integrated microfluidic liquid chromatography devices and systems include multiple separation columns integrated into a single substrate.
|High-throughput multi laser wave mixing methods and apparatus|
This invention relates to methods and apparatus of a combination of laser wave mixing technology with diagnostic flow technologies with embodiments describing supercritical fluid chromatography. The combination of these technologies along with minute detection levels have not yet been seen in the field..
|Lc-ms configuration for purification and detection of analytes having a broad range of hydrophobicites|
Systems, apparatuses, kits, and methods for purification and analysis of analytes having a broad range of hydrophobicities by liquid chromatography-mass spectrometry (lc-ms). Using one set of liquid chromatography columns, one set of mobile phase buffers, and, optionally, a single ionization method (e.g., electrospray ionization), a wide range of analytes can be purified and analyzed on a liquid chromatography-mass spectrometry (lc-ms) system.
|Fast gas chromatograph method and device for analyzing a sample|
A fast gas chromatograph (gc) method and device for obtaining fast gas chromatography analysis, in which a capillary gas chromatography column is inserted into a resistively heated metal tube located mostly outside a heated oven, which serves as a heated transferline to a flexible column that enters a resistively heated metal tube from a gas chromatograph injector and exits into a gas chromatograph detector. The resistively heated metal tube of the fast gc device has an internal diameter that is over twice the external diameter of the gc column so as to enable the insertion of several capillary gc column loops.
|Method of purifying protein|
The present invention relates to a method for purifying a protein by separating the protein from impurities in a non-adsorption mode using an activated carbon. In particular, the present invention relates to a method for purifying an antibody using the activated carbon instead of protein a affinity chromatography..
|Method for preparing a concentrate of factor xi|
The invention concerns a concentrate of human factor xi having high specific activity prepared using a method comprising a filtration-adsorption step and a chromatography step on cation exchange resin. The concentrate obtained is fully adapted for therapeutic use as substitution therapy in cases of factor xi deficiency..
|Method for identification and purification of multi-specific polypeptides|
The document pertains to a method for the purification of a ternary mixture of dimeric antibodies of the type aa, ab, bb, characterised in that for the separation of the three components and in particular for the isolation of the multi-specific fraction ab multicolumn counter current solvent gradient purification chromatography with a stationary phase load of more than 1 mg antibody mixture per millilitre stationary phase is used. It furthermore relates to a method for the identification of in particular bispecific antibody systems, which are particularly suitable for the application of such a purification method..
|Method for preparing a depleted plasma material consisting of one or more thrombogenic factors|
The invention concerns a method for preparing a plasma product depleted of one or more thrombogenic factors, comprising the combination of at least two steps chosen from among an ethanol fractionation step, a filtration-adsorption step, a precipitation step with caprylic acid and a chromatography step on ion exchange resin.. .
|High pressure pump with reduced seal wear|
Described are embodiments of a pump that can be used, for example, in liquid chromatography applications. The pump includes a seal wash housing, pump head and seal assembly.
|Switching valve for liquid chromatography|
The invention relates to a switching valve for liquid chromatography having a stator in which multiple ports are formed. Each port is formed by in each case one duct which is connected at one end to in each case one connection port and which, at the other end, has a predetermined port opening cross section at a stator face surface of the stator.
|Aromatic polycarbonate resin composition for light guide plates, and light guide plate|
There is provided an aromatic polycarbonate resin composition exhibiting excellent mechanical strength, transfer property, light transmittance, thermal stability and moldability which is capable of being molded into thin-wall products and large-size products, as well as a light guide plate produced from the resin composition. The present invention relates to an aromatic polycarbonate resin composition for light guide plates, comprising an aromatic polycarbonate resin having a viscosity-average molecular weight of 13,000 to 15,000, and a ratio of a weight-average molecular weight to a number-average molecular weight (mw/mn) of 1.5 to 2.7 in terms of polystyrene as measured by gel permeation chromatography; and a stabilizer and a releasing agent blended in the aromatic polycarbonate resin, as well as a light guide plate produced from the resin composition..
|Switching valve for high-performance liquid chromatography|
A switching valve is described that has a stator which is arranged in a housing and which has multiple connection ports. The switching valve also has a rotatable rotor which is arranged in the housing and which, in predetermined switching positions defined by associated angular positions, interacts with the stator for the fluidic connection or separation of predetermined connection ports.
|Switching valve for liquid chromatography|
A switching valve includes a stator and a rotor. The stator includes multiple connection ports.
|Switching valve for liquid chromatography|
A switching valve includes a stator and a rotor. The stator includes multiple connection ports.
|Method for separating and purifying ginkgolide c from root bark of ginkgo|
Disclosed is a method for separating and purifying ginkgolide c from root bark of ginkgo. The method comprises: (1) extracting the root bark of ginkgo with ethanol; (2) concentrating the resulting extract under vacuum to remove ethanol; (3) separating the concentrate by macroporous resin column chromatography; (4) after the concentrate being loaded on the column, washing the column with pure water to remove impurities, and then eluting the column with an ethanol solution; (5) concentrating the eluate under vacuum to dryness to obtain a yellow crude extract; (6) heating the crude extract in water to boiling to form a solution, and then refrigerating the solution; (7) concentrating the supernatant solution and filtering under vacuum to obtain a mixed crude crytal of ginkgolides; (8) dissolving the crude crystal in ethanol to form a supersaturated solution, refrigerating and crystallizing the solution to remove ginkgolides a and b; (9) concentrating and recrystallizing the mother liquor to obtain a crystal of ginkgolide c; and (10) recrystallizing the crystal with ethanol several times to obtain a high-purity crystal of ginkgolide c..
|Novel process for the preparation of a virus-inactivated fv concentrate starting from human plasma, scalable to industrial level|
The present invention provides a process for purifying fv starting from human plasma or a fractionation intermediate thereof, that is simple, scalable to the industrial level and relatively inexpensive compared to the methods described in the literature to date. The invention consists of the use of two anion exchange chromatography steps, the first of which has the purpose of separating the fv from the ptc component factors, while the second has the purpose of isolating the protein of interest from the majority of plasma proteins by means of selective interaction with the weak anion exchange support used.
|Method and apparatus for performing retention time matching|
A method for matching a precursor ion with one or more related product ions includes providing input data sets obtained from sample injections, each of the data sets including a precursor ion and one or more product ions, normalizing the input data sets in accordance with a single retention time for the precursor ion, determining which product ions are within a predetermined retention time window with respect to the single retention time, and, if a product ion is within the predetermined retention time window for a specified number of the input data sets, determining that the product ion is related to the precursor having the single retention time. An apparatus for analyzing a sample includes a chromatography module, a mass-spectrometry module in communication with the chromatography module, and control unit in communication with the chromatography module and the mass-spectrometry module..
|Liquid chromatography component|
The present invention aims to provide a liquid chromatography component including a column and a prefilter, which is hard to cause an increase of supplied liquid pressure even when the measurement of a sample is repeated. The present invention is a liquid chromatography component, which includes: a column with filler particles filled therein; and a prefilter, the filler particles having an average particle size in the range of 2 to 20 μm, the prefilter having a filtering particle size in the range of ⅙ to ⅓ of the average particle size of the filler particles..
|Narrow bore porous layer open tube capillary column and uses thereof|
A polymer-based plot capillary column prepared by in situ copolymerization of a functional monomer and a crosslinking monomer, which enhances the strength of the polymer matrix, is disclosed. Also disclosed is a system comprising the polymer-based plot column coupled to a mass flow or concentration sensitive detector, for carrying out a chemical analysis method on samples separated by liquid chromatography using the column, and a process for using the system.
|Chromatographic system quality control reference materials|
The invention provides compositions and methods for chromatographic analysis and system quality control. Compositions can include a reference material that comprises a standardized formulation of two or more compounds that can be used for benchmarking and troubleshooting the chromatographic system (e.g., which is not simply a standard solution for a particular analyte of interest).
|Novel alkali-resistant variants of protein a and their use in affinity chromatography|
The present invention relates to immunoglobulin (ig)-binding proteins with alkali-resistance properties. In one embodiment, the present invention provides for a variant of an ig-binding protein, the variant comprising the ig-binding protein having at least one asparagine residue substituted with a histidine, a serine, an aspartic acid or a threonine residue.
|Method of detecting pancreatic disease and pancreas testing kit|
A pancreatic disease is tested for with high sensitivity even with simple equipment and a simple procedure. Provided is a method of detecting pancreatic disease including detecting a concentration of s100p in at least one of a pancreatic juice and a body fluid containing pancreatic juice collected from a test subject by immunochromatography.
|Filler for ion exchange chromatography and method for separating and detecting nucleic acid strand|
The present invention aims to provide a filler for ion exchange chromatography which can sufficiently detect nucleic acid chains that differ in sequence of bases or nucleic acid chains that differ by a single base substitution. The present invention also aims to provide a method for separating and detecting a nucleic acid chain using the filler for ion exchange chromatography.
|Connection assembly for ultra high pressure liquid chromatography|
A fitting assembly having a nut, a ferrule, and a ferrule tip that may be assembled by an operator. The fitting assembly includes a nut with first and second ends, with the second end adapted to receive the first end of a ferrule, and a ferrule tip with a first end having an externally tapered portion adapted to abut the second end of the ferrule and a second end adapted to be received in a component or fitting of a liquid chromatography system.
|Optically heated analyte desorber for gas chromatography analysis|
Analytes are rapidly desorbed from a carbonaceous sorbent powder with improved quantitation and reduced analyte re-adsorption, thermal degradation, and rearrangement. The sample is distributed in a thin layer onto a desorption surface within a chamber.
|Micro-device for analysis by gas phase chromatography offering great compactness|
A thermoelectric module, the hot face heating the chromatography micro-column and the cold face cooling the detection module.. .
|Formulations of bendamustine|
Long term storage stable bendamustine-containing compositions are disclosed. The compositions can include bendamustine or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable fluid which can include in some embodiments peg, pg or mixtures thereof and an antioxidant or chloride ion source.
|Chromatographic separation material|
The chromatographic separation material is a cyclofructan or a derivative of cyclofructan covalently bonded to a cross-linked, organic polymer. The separation material works well in hydrophilic interaction liquid chromatography..
|Hplc frit filter assembly|
An apparatus and method for creating a high pressure chromatography frit filter assembly is described. The frit is positioned in bondable contact with a polymer ring and then the frit is subject to inductive or targeted heating to cause the frit material to heat the adjacent polymer ring from the inside out.
|Liquid chromatography column, and method for analyzing hemoglobin|
The present invention provides a column for liquid chromatography having a column pipe and an end fitting, wherein the column pipe and the end fitting comprise polyethylene or polymethylmethacrylate.. .
A method for purifying a polypeptide by ion exchange chromatography is described which involves changing the conductivity and/or ph of buffers in order to resolve a polypeptide of interest from one or more contaminants.. .
|Analytical methods for analyzing and determining impurities in dianhydrogalactitol|
An improved analytical method for analysis of dianhydrogalactitol preparations provides a method for determining the purity of dianhydrogalactitol and detecting impurities in preparations of dianhydrogalactitol, as well as identifying any such impurities. The method employs high performance liquid chromatography (hplc), in particular, hplc with evaporative light scattering detection (elsd); the hplc can be followed by tandem mass spectroscopy.
|Protein affinity tag and uses thereof|
This invention concerns isotopically coded or non-isotopically coded affinity-tags for analysis of certain target molecules in complex samples, in particular for mass spectrometric analysis of proteomic samples. The affinity-tags have the following general formula x-spacer-opo3h2, wherein x is a functional group or moiety capable of reacting with a functional group of a protein, peptide, dna, lipid, sugar and/or steroid.
|Adhesive resin composition and multilayer structure using the same|
There is provided an adhesive resin composition suitable for a multilayer structure which retains sufficient adhesive strength even when in contact with gasoline or light gas oil, and has excellent long-term durability and durability in high-temperature fuels and excellent adhesive strength at high temperature. The adhesive resin composition of the invention includes a modified ethylene polymer (al) which is graft-modified with an unsaturated carboxylic acid or a derivative thereof and which has a density of 930 to 980 kg/m3, and an unmodified ethylene polymer (a2) having a density of 910 to 929 kg/m3, wherein the adhesive resin composition has a melt flow rate (mfr) [astm 0 1238 (temperature: 190° c., 2160 g load)] of 0.1 to 3 g/10 min and a density of 920 to 930 kg/m3 and has an elution amount of 60 wt % or less at 85° c.
|All-in-one sample preparation device and method|
Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet.
|Polyetherimide resins with very low levels of residual contamination|
Compositions and methods for producing compositions comprising a monoamine-endcapped polyimide component. Based on a gas chromatography mass spectroscopy analysis of a surface rinse of the composition performed at room temperature, the composition can have at least one surface with less than or equal to 5 ppb releasable phosphorous residuals, and less than or equal to 5 ppb releasable volatile organic compound residuals.
|Methods of producing competitive aptamer fret reagents and assays|
Methods are described for the production and use of fluorescence resonance energy transfer (fret)-based competitive displacement aptamer assay formats. The assay schemes involve fret in which the analyte (target) is quencher (q)-labeled and previously bound by a fluorophore (f)-labeled aptamer such that when unlabeled analyte is added to the system and excited by specific wavelengths of light, the fluorescence intensity of the system changes in proportion to the amount of unlabeled analyte added.
|Novel purification of non-human antibodies using protein a affinity chromatography|
Disclosed herein are compositions and methods for the isolation and purification of antibodies from a sample matrix. In particular, the present invention relates to compositions and methods for isolating and purifying antibodies exhibiting weak binding strength and low binding capacity for protein a resin.
|Separation process by means of high flow continuous chromatography and corresponding device|
A method and device are provided for separating fractions of a mixture that is to be separated by liquid phase chromatography. The method includes the steps of: multiple injections of the mixture to be separated, where the injections are made successively into a chromatography column after time intervals a; multiple decanting operations, wherein the fractions of said column are decanted successively after time intervals a, generating an eluate enriched with the fraction of interest, which is created from at least one of said decanted fractions; multiple injections of the eluate collected in the preceding step, wherein the injections of the eluate are carried out successively after time intervals b into a second chromatography column; and multiple decanting operations, wherein the fractions from said second column are decanted successively after time intervals b..
|Support for affinity chromatography and method for isolating immunoglobulin|
Wherein r represents a polypeptide consisting of 4 to 30 amino acid residues that contains an amino acid sequence represented by atk or ask; and r2 represents a polypeptide consisting of 50 to 500 amino acid residues containing an immunoglobulin-binding domain consisting of an amino acid sequence represented by seq id no: 1 or seq id no: 2, the partial sequence thereof, or an amino acid sequence having 70% or more identity to these sequences; with the proviso that a terminus at which r2 binds to r is c-terminus or n-terminus of the immunoglobulin-binding domain.. .
|Waterborne coating composition|
The invention relates to an aqueous dispersion for use as open time improver in a coating composition which aqueous polymer dispersion comprises a first polymer having a number average molecular weight (mn) of from 2,000 to 120,000 (determined by gel permeation chromatography using a mixture of tetrahydrofurane and acetic acid as eluent), an acid value of from 30 to 150 mg koh/g, and an ethylene-oxide wt % (on total solid polymer) of from 1 to 20 wt %, said first polymer dispersion being obtainable by free radical polymerization of a monomer mixture in the presence of at least one free-radical initiator and at least one surfactant, said monomer mixture comprising : a) 5 to 20 wt %, preferably 7 to 10 wt %, acid functional ethylenically unsaturated monomers or precursors thereof or ethylenically unsaturated monomers comprising ionic group precursors; b) 5 to 25 wt %, preferably 7 to 20 wt %, ethylenically unsaturated monomers containing polyethylene oxide, polyethylene glycol or mono-alkoxypolyethylene glycol moeity c) up to 90 wt % of non-ionic ethylenically unsaturated monomers other than a) or b); d) 0 to 10 wt % ethylenically unsaturated monomers with a functional group for cross-linking e) 0 to 10 wt % of chain transfer agents; f) up to 90 wt % non-ionic ethylenically unsaturated monomers other than c), wherein 30 to 90 wt %, more preferably 60 to 80 wt % comprise crosslinkable groups or precursors thereof; wherein the sum of a) through f) is 100 wt %. The invention further relates to a method for making the first polymer dispersion, the use of said aqueous dispersion as an open time improver in a coating composition, to aqueous coating compositions comprising a blend of at least a first aqueous polymer dispersion and a second aqueous polymer dispersion comprising a film-forming second polymer and to a method for making said coating composition..
|Electrophotographic photosensitive member, method of producing electrophotographic photosensitive member, process cartridge, and electrophotographic apparatus|
Provided is an electrophotographic photosensitive member in which an undercoat layer is a layer of which at least one selected from the group consisting of a compound represented by the formula (1), a compound represented by the formula (2), a compound represented by the formula (3) and a compound represented by the formula (4) can be detected by gas chromatography analysis when the undercoat layer is heated at 150° c. For 60 minutes by headspace method..
|Rheology modifiers for modifying the rheological behaviour of coating compositions|
The present invention provides rheology modifiers, which are water-soluble polymers having a weight average molecular weight (mw) of at least 1′000′000 g/mol and an intrinsic viscosity of at least 2.5 dl/g, both as determined by size exclusion chromatography, and wherein the water-soluble polymers are in the form of solid particles. It also provides mixtures of rheology modifiers comprising a first rheology modifier, which is a water-soluble polymer having a weight average molecular weight (mw) of at least 1′000′000 g/mol and an intrinsic viscosity of at least 2.5 dl/g, both as determined by size exclusion chromatography, and a second rheology modifier, which has a weight average molecular weight of from 2′000 g/mol to 800′000 g/mol.
|Purification of iduronate-2-sulfatase|
The present invention provides, among other things, improved methods for purifying i2s protein produced recombinantly for enzyme replacement therapy. The present invention is, in part, based on the surprising discovery that recombinant i2s protein can be purified from unprocessed biological materials, such as, i2s-containing cell culture medium, using a process involving as few as four chromatography columns..
|Pecvd coating of chromatography vials|
A chromatography vial is disclosed comprising a thermoplastic wall and a coating on the wall. The coating comprises a pecvd barrier coating or layer of siox, where x is from 1.5 to 2.9, on the interior surface of the vial wall.
|Colorant compound derived from genipa americana genipin and glycine|
The present invention provides colorant compounds and its molecular structural formulas and methods of isolation of the colorant compounds derived from a reaction of genipa americana genipin and glycine. The novel compounds were obtained from multiple fractioning by chromatography of the reaction resulting material.
|Purified cardiogenin isomer and related methods|
A cardiogenin major isomer is obtained from a methanol extract of geum japonicum and separated from its minor isomer. The separation of the two isomers can be achieved by chiral phase chromatography, e.g., using a chiralpak® ic™ column.
In a method for detecting a glucagon-secretin family peptide in a sample, the sample is contacted with a cleaving agent capable of digesting the glucagon-secretin family peptide by cleaving a peptide bond of at least one aspartic acid within the glucagon-secretin family peptide and thereby generating a plurality of peptide fragments, at least one of the peptide fragments containing an n-terminal end of the glucagon-secretin family peptide. The peptide fragment that contains the n-terminal end of the glucagon-secretin family peptide is then detected using liquid chromatography and mass spectrometry.
|Chromatography column stand|
A method for conducting maintenance on a chromatography column (1; 51), said column comprising a column tube (32), a top end cell (20) and a bottom end cell (26), where during chromatography said column tube (32) is secured between said top end cell (20) and said bottom end cell (26). Said method comprises the step of rotating at least the top end cell (20) 180 degrees around a horizontal axis before maintenance is conducted to the top end cell (20)..
|Pump head outlet port|
Pumps used in liquid chromatography applications employ actuators for pressurizing and moving liquid. An actuator includes a pump head having a chamber, a gland, a movable rod extending through the gland into the chamber of the pump head, and a plunger seal disposed in the gland around the rod for sealing against fluidic leakage from the chamber.
|Flow through isolation valve for high pressure fluids|
A flow through isolation valve having a stationary member; a movable member, with a surface of the stationary member interfacing with a surface of the movable member; and at least one pin isolation valve. The pin isolation valve has a flow through internal conduit and is movable so that the internal conduit can fluidically communicate with at least one blank opening in the movable member and with a flow through internal conduit in the movable member.
|Method for producing di(arylamino)aryl compound and synthetic intermediate therefor|
Provided is a method for producing a di(arylamino)aryl compound that has superior inhibitory activity against the kinase activities of eml4-alk fusion protein and mutant egfr protein and is useful as an active ingredient in pharmaceutical compositions for cancer treatment. The production method includes no purification step using silica gel column chromatography or no step possibly producing a mutagenic mesylate ester as a by-product, greatly improves overall yield, and is high yield and low cost and suitable for the industrial production of pharmaceutical products.
|Purification of diphtheria toxoid|
The invention disclosed is a method of purifying diphtheria toxoid (dt) by hydrophobic interaction chromatography (hic). The chromatographic method of the present invention provides an effective removal of contaminating glycans present in carrier protein dt and thereby provides a highly purified form of carrier protein dt for the production or preparation of polysaccharide protein conjugate vaccines..
|Protein purification methods to reduce acidic species|
The instant invention relates to the field of protein production and purification, and in particular to compositions and processes for controlling the amount of charge variants, aggregates, and fragments of a protein of interest, as well as host cell proteins, present in purified preparations by applying particular chromatography conditions during such protein purification.. .
|Affinity chromatography matrix|
The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the matrix containing the immunoglobulin containing proteins adsorbed thereon; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins.
|Methods for purifying monosaccharide mixtures containing ionic impurities|
Disclosed herein are methods for separating ionic impurities from monosaccharide processing streams using simulated moving bed chromatography.. .