This page is updated frequently with new Chromatography-related patent applications.
|| List of recent Chromatography-related patents
An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns.. .
Therapeutic Proteins International, Llc
|Methods of resolving racemic mixture to obtain (-)-huperzine a|
A method of resolving a racemic mixture of (±)-huperzine a to (−)-huperzine a includes: separating the (−)-huperzine a from the racemic mixture of (±)-huperzine a by chiral high performance liquid chromatography (hplc), the chiral hplc being performed utilizing a mobile phase including a solution including an alcohol and one selected from dichloromethane, trichloromethane, and a mixture thereof, and the chiral hplc being performed utilizing a chiral stationary phase including a polysaccharide derivative.. .
Amphastar Pharmaceuticals Inc.
|Method for measuring relative content of gram-negative bacteria in active sludge|
A method for measuring a relative content of gram-negative bacteria in an active sludge, the method including: 1) breaking zoogloeas in the active sludge; 2) isolating 2-keto-3-deoxyoctonic acid (kdo) from free bacteria and cell debris; 3) performing fluorescence labeling on the isolated kdo to obtain a fluorescent derivative; and 4) isolating the fluorescent derivative by high performance liquid chromatography (hplc) and conducting quantitative measurement by fluorescence to obtain the relative content of the gram-negative bacteria in the active sludge.. .
|Prediction of fuel properties|
A system is described that includes a known fuels database of data from gas chromatography-mass spectrometry analyses of a library of fuels with known fuel properties for a multiple known fuel samples. Gas chromatography-mass spectrometry equipment can acquire gas chromatography-mass spectrometry data for an unknown fuel sample.
The Government Of The United States Of America, As Represented By The Secretary Of The Navy
|Method and device for determining a composition of a gas sample processed by means of gas chromatography|
A method for determining a composition of a gas sample comprising the following steps: running the sample through a processing chain comprising a gas chromatography column and a gas detector arranged at the outlet of the gas chromatography column; obtaining, at the output of the detector, a representative electrical signal of the composition; determining the gas composition by processing this electrical signal. The determination of the composition of the sample is performed on the basis of a combination of a direct probabilistic model of the chromatography column, this model comprising at least one law of probability defining, for at least one gas species liable to be found in the sample, a probability at each moment that a molecule of this gas species is discharged from the chromatography column, and an impulse response model of the detector.
Commissariat A L'energie Atomique Et Aux Energies Alternatives
|Automated calibrating a chromatography system and analysis of a sample|
An automated method of calibrating a chromatography system and analyzing a sample is described. The method includes forming diluted standard solutions that are injected into a chromatography column.
|Micro-machined frit and flow distribution devices for liquid chromatography|
A micro-machined frit is provided for use in a chromatography column, having a substrate with a thickness, and holes extending through the thickness and providing fluid communication through the substrate. A micro-machined flow distributor is provided for use in a chromatography column having a substrate, holes extending through the substrate, and channels in fluid communication with the holes.
Agilent Technologies, Inc.
|Cation exchanger for liquid chromatography, process for producing same, and use thereof|
A cation exchanger for liquid chromatography, comprising non-porous particles and, immobilized to the surface thereof, a polyacrylic acid into which a dicarboxylic acid compound having an amino group is introduced by an amide bond.. .
|Nuclear magnetic resonance implemented synthetic indole and indazole cannabinoid detection, identification, and quantification|
The present invention provides a method for detecting synthetic indole and indazole cannabinoids in a sample known or suspected to contain a synthetic indole or indazole cannabinoid. A deuterated solvent is added to the solid sample, creating a suspension.
|Composition for pressure-sensitive adhesive and pressure-sensitive adhesive sheet|
A composition for a pressure-sensitive adhesive, includes (a) a (meth)acrylic ester polymer having a trithiocarbonate structure, having hydroxyl groups at both ends of a molecule and having a weight-average molecular weight (mw), as measured by gel permeation chromatography, of 50,000 to 300,000, and (b) an isocyanate compound having an average number of isocyanate groups in one molecule being more than 2.. .
Soken Chemical & Engineering Co., Ltd.
Continuous multistep process for purifying antibodies
The invention provides a three-step chromatography process for small and large-scale purification of proteins, specifically monoclonal antibodies, using only four buffer solutions made from a mother solution.. .
Separation of recombinant polyclonal antibody multimers with minimal separation of monomers
The invention provides a method for removing multimers from a preparation of recombinant polyclonal antibodies (rpabs) while maintaining the ratio of monomers within a narrow range. The invention provides a method of separating recombinant polyclonal antibody multimers with minimal separation of monomers comprising subjecting a mixture comprising a plurality of monoclonal antibodies to at least one separation process selected from the group consisting of multi-modal chromatography, apatite chromatography, and hydrophobic interaction chromatography thereby producing an antibody monomer preparation that is substantially free of multimers..
Isolation and purification of antibodies using protein a affinity chromatography
Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise ph viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography, preferably protein a affinity, ion exchange chromatography, and hydrophobic chromatography.
Method for purifying antibody protein
A method for purifying a biologically active substance from a solution mixture containing impurities and the biologically active substance, in which an ion exchange chromatography carrier comprising a matrix and a copolymer containing at least n-isopropylacrylamide as a monomer unit and immobilized to a surface of the matrix is used, and the solution mixture is allowed to flow through a container storing the carrier at a uniform temperature, thereby recovering the biologically active substance.. .
Asahi Kasei Medical Co., Ltd.
Functionalized 4- and 5-vinyl substituted regioisomers of 1, 2, 3-triazoles via 1, 3- dipolar cycloaddition and polymers thereof
The present invention provides novel functionalized mixtures 4- and 5-vinyl substituted regioisomers of 1,2,3-triazoles via 1,3-dipolar cycloaddition. Functionalized alkyne moieties with a terminal alcoholic functionality are reacted with functionalized organic moieties with a terminal leaving group and an azide to provide an alcoholic functionalized mixture of 4- and 5-substituted regioisomers of 1,2,3-triazole moieties.
Isp Investments Inc.
Preparation 1-palmitoyl-3-acetylglycerol, and preparation 1-palmitoyl-2-linoleoyl-3-acetylglycerol using same
Disclosed are a method for preparing 1-palmitoyl-3-acetylglycerol in high purity and high yield without a purification process using a column chromatography, and a method for preparing 1-palmitoyl-2-linoleoyl-3-acetylglycerol in high purity and high yield using the same as a key intermediate. The method for preparing 1-palmitoyl-3-acetyl glycerol comprises the steps of: forming a reaction mixture including 1-palmitoyl-3-acetyl glycerol of the formula 1 in the specification by reacting 1-palmitoylglycerol of the formula 2 in the specification and an acetylating agent; and separating the optically active 1-palmitoyl-3-acetylglycerol by crystallizing the reaction mixture in a saturated hydrocarbon solvent having 5 to 7 carbon atoms..
Enzychem Lifesciences Corporation
Thin-layer chromatography plate, producing such a plate, and performing a thin-layer chromatography separation
The present invention relates to a thin-layer chromatography plate at least consisting of a support and a sorbent layer, where the sorbent layer comprises at least one siloxane oligomer, to a process for the production thereof, and to a method for carrying out a thin-layer chromatographic separation using said thin-layer chromatography plate.. .
Merck Patent Gmbh
Multi-measurement flow cell assembly for liquid chromatography
A detector for detecting constituents of a liquid for use in liquid chromatography is disclosed. The detector includes a first optical flow cell body and a second optical flow cell body, each having a channel therethrough that allows passage of a liquid from an inlet port to an outlet port.
Gas chromatography (gc) column heater
A gas chromatography (gc) column heating apparatus is described. The gc column heating apparatus includes a first substrate; a heating element disposed over the first substrate; and a second substrate.
Agilent Technologies, Inc.
Gas chromatography (gc) column heater
An apparatus for heating a gc column is described. The apparatus includes first and second temperature sensors.
Agilent Technologies, Inc.
Sample dispenser for an analytical device
A sample dispenser for an analysis device, in particular for an analysis device working according to the principle of liquid chromatography, in particular high pressure liquid chromatography, or gas chromatography, comprising a sample intake for receiving a sample to be analyzed, an inlet through which an eluent can be supplied, an outlet, and an injection valve arrangement, which can be switched at least from an intake position to an injection position, wherein, at least in the intake position and in the injection position, the inlet is in fluid connection with the outlet to deliver the eluent—if applicable, having the sample added thereto—at least partly to the outlet, wherein, in the intake position, the sample intake is separated from the eluent in a fluid-tight manner, and wherein, in the injection position, the sample in the sample intake can combine with the eluent, wherein the sample dispenser is configured as a disposable component.. .
Hamilton Bonaduz Ag
Facile and selective perfluoro-and polyfluoroarylation of meldrums acid
This disclosure relates generally to the facile and selective mono-perfluoro and polyfluoroarylation of meldrum's acid to generate a versatile synthon for highly fluorinated alpha-phenyl acetic acid derivatives which provide straightforward access to fluorinated building blocks. The reaction takes place quickly and all products were isolated without the need for chromatography.
The Board Of Regents For Oklahoma State University
Process for preparing and separating monodisperse polyethylene glycol
A process for preparing and separating a monodisperse polyethylene glycol, including: dissolving a starting material of polyethylene glycol into an organic solvent for reaction with a compound represented by general formula i under catalytic action of an alkaline substance, to prepare a mixture of derivatives of polyethylene glycol having different polarities; separating the mixture of derivatives of polyethylene glycol by means of silica gel column chromatography, to obtain a monodisperse derivative of polyethylene glycol; and hydrolyzing the derivative of polyethylene glycol to obtain a monodisperse polyethylene glycol. The starting material is industrially prepared polyethylene glycol with a wide source and low cost, which provides a guarantee for popularization and application of a monodisperse polyethylene glycol product..
Opegchem Company, Inc.
Continuous chromatography method and system
Continuous chromatography methods and systems are disclosed, wherein a tangential flow filtration device used in association with at least a first chromatography device and a second chromatography device, concentrate a product of interest, which improves sorbent utilization.. .
Chromatography method and system
chromatography methods and systems are disclosed, wherein a tangential flow filtration device is used to concentrate a product of interest, which improves sorbent utilization.. .
Thermally modulated variable restrictor for normalization of dynamic split ratios
A chromatography system includes a separation column that separates a sample carried by a compressible mobile phase flow into analytes and a splitter in fluidic communication with the separation column to receive and divide the compressible mobile phase flow into first and second mobile phase streams in accordance with a split ratio. A thermally modulated variable restrictor is coupled between the splitter and a detector.
Water Technologies Corporation
Controllable injector sample dilution for a liquid chromatography system
Described are a method and a system for injecting a sample into a flow of a liquid chromatography system. The method includes combining a flow of a sample and a flow of a mobile phase to create a diluted sample in the system flow.
Waters Technologies Corporation
Microstructure separation filters
Microstructure separation filters are provided herein, as well as chromatography and other separation devices. An exemplary filter device includes a microstructure filter has a plurality of layers of alternating sacrificial and/or structural material which have been etched to create inlet channels and outlet channels.
Imagine Tf, Llc
Purification of polysaccharide protein conjugates
The invention describes a method of purifying polysaccharide protein conjugates using mixed mode chromatography. The method involves contacting a crude polysaccharide protein conjugate with a mixed mode resin comprising an inert porous shell and an activated core under conditions of low conductivity that allow binding of the contaminants and collecting the unbound polysaccharide protein conjugate in a flowthrough..
Shantha Biotechnics Private Limited
Simple the detection of sulfur compounds in crude oils and diesel
A liquid phase microextraction (lpme) method was developed for the determination of sulfur compounds in crude oils and its fractions (e.g diesel). Wide ranges of sulfur compounds including benzothiophene, dibenzothiophene and their derivatives were used as model compounds for extraction.
King Abdulaziz City For Science And Technology
Quantification of asphaltene inhibitors in crude oil using thermal analysis coupled with mass spectrometry
The present invention generally relates to a novel method for the characterization and quantification of involatile macromolecules in hydrocarbon samples of crude oil. More particularly, the method utilizes pyrolysis/gas chromatography/mass spectrometry (py/gc/ms, py/gc/ms/ms or py/gc/triple quad ms) to detect and quantify polymeric asphaltene inhibitors in crude oil..
Ecolab Usa Inc.
Plug unit and connection system for connecting capillary tubes, especially for high-performance liquid chromatography
A plug unit for connecting capillary tubes includes a plug housing that has an axial borehole, a plug capillary tube that projects through the axial borehole, and a sealing element that surrounds the plug capillary tube. The front end of the plug capillary tube is sealed by an elastic and/or plastic deformation of the sealing element against the capillary tube receptacle opening of a bushing unit.
Dionex Softron Gmbh
Automated analysis systems
The disclosure relates to systems and methods for performing high performance liquid chromatography (hplc) on a liquid sample. The methods include automatically, using a computer, controlling a liquid sample including a plurality of components to flow through a first flow path in a system for hplc.
The General Hospital Corporation
Compositional analysis of high boiling point mixtures by comprehensive two-dimensional gas chromatography
The thermally modulated, two-dimensional gas chromatographic separation of a mixture of compounds with boiling points higher than 260° c. Uses a cold trap with a gas jet at ambient temperatures..
Exxonmobil Research And Engineering Company
Ultrathin layer chromatography plates comprising electrospun nanofibers
An ultrathin-layer chromatography plate comprising a stationary phase including electrospun composite nanofibers comprising a polymer and at least one of multi-walled carbon nanotubes, edge-plane ordered carbon nanorods and amorphous carbon nanorods, wherein the stationary phase has a thickness between about 5 μm and about 30 μm.. .
Ohio State Innovation Foundation
The present invention is to provide a toner that has a total volatile organic component content (tvoc content) reduced to a low level, is less likely to cause fog even when used in either a low-temperature and low-humidity environment or a high-temperature and high-humidity environment, does not cause filming even in continuous printing, and is excellent in printing durability. Disclosed is a toner comprising a binder resin, a wax, a charge control resin and a colorant, wherein, as a result of gas chromatography analysis of a supernatant obtained by dissolving the charge control resin in a first organic solvent to prepare a charge control resin solution and then adding a second organic solvent to the charge control resin solution to precipitate a polymer component and to obtain the supernatant, a first total amount of components detected in a range of a peak detecting time of hydrocarbons having 5 to 9 carbons is 500 ppm or less in terms of styrene; a second total amount of components detected in a range of a peak detecting time of hydrocarbons having 10 to 18 carbons is 5,000 ppm or less in terms of styrene; and an amount of a component corresponding to a maximum peak of peaks of hydrocarbons having 10 to 18 carbons is 3,000 ppm or less in terms of styrene..
Method for establishing shenqi fuzheng injection fingerprint spectrum
A method for establishing a shenqi fuzheng injection fingerprint spectrum, comprising: employing an ultra-high voltage liquid chromatography mass spectrometer to test the shenqi fuzheng injection, the chromatography conditions including: chromatographic column: agilent zorbax eclipse plus c18, 2.1 mm×100 mm, 1.8 μm; mobile phase: mobile phase a is 0.1% formic acid aqueous solution, and mobile phase b is 0.1% formic acid acetonitrile solution; employing gradient elution procedure as follows: 0-0.5 min, 95% of mobile phase a, and 5% of mobile phase b; 0.5-10 min, 95%-75% of mobile phase a, and 5%-25% of mobile phase b; 10-15 min, 75%-45% of mobile phase a, and 25%-55% of mobile phase b; 15-18 min, 45%-0% of mobile phase a, and 55%-100% of mobile phase b; and 18-20 min, 0% of mobile phase a, and 100% of mobile phase b.. .
Li Min Pharmaceutical Factory Of Livzon Pharmaceut Ical Group
Injector sample dilution for a liquid chromatography system
Described are a method and a system for diluting a sample at a location of injection in a liquid chromatography system. The method includes loading a sample into a first fluid channel, separating a flow of a mobile phase into a first flow in the first fluid channel and a second flow in a second fluid channel, and combining the sample that is displaced from the first fluid channel and the mobile phase exiting the second fluid channel at the location of injection into the system flow to thereby generate a diluted sample in the system flow.
Waters Technologies Corporation
Two-dimensional liquid chromatography system for heart-cut method
Provided is a two-dimensional liquid chromatography system, and more particularly, a two-dimensional liquid chromatography system capable of performing both independent one-dimensional separation through a reversed-phase or normal-phase chromatography method and two-dimensional separation for removing a matrix effect in a single system.. .
Korea Research Institute Of Standards And Science
Surface neutralization of apatite
The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution.. .
Bio-rad Laboratories, Inc.
Innovative auto cut system for 10b isotope of boron enrichment using continuous ion exchange chromatography
The present invention relates to process of enrichment of boron isotopes by ion exchange chromatography by “auto cut” system using continuous ion exchange chromatography. The process comprises development of borate band in the ion exchange chromatographic column; eluting said band without any regeneration in a manner where said ion exchange chromatographic columns comprises 3-9 chromatographic columns in groups of three columns each and connected in series with silicon tubes; and wherein said eluting agent is provided to first column of each said group of three columns; and wherein said enriched boron isotopes are obtained upto 90%..
Secretary, Department Of Atomic Energy
Thin polarizing film, optical laminate with thin polarizing film, and production thin polarizing film
The thin polarizing film is produced by forming a polyvinyl alcohol-based resin layer on a thermoplastic resin substrate. The thin polarizing film has a thickness of 10 μm or less, a single axis transmittance of 42.0% or more, a polarization degree of 99.95% or more, and an iodine content of 17.6 grams per 400×700 square millimeters or less, which is measured by an ion chromatography method..
Glycoproteins having lipid mobilising properties and therapeutic applications thereof
A biologically active lipid mobilising agent for use in therapy is disclosed which has the properties and characteristics of a zn-α2-glycoprotein, or of a fragment thereof having an apparent molecular mass mr greater than 6.0 kda as determined by gel exclusion chromatography. Methods of isolation and purification from biological material are also disclosed together with uses of the material for making up pharmaceutical compositions, especially pharmaceutical compositions useful for treating mammals to achieve weight reduction or for controlling obesity.
Purification of recombinant alpha galactosidase a
In one embodiment, the invention provides a method of purifying recombinant alpha-galactosidase a. The method includes obtaining a lysate from cells recombinantly expressing alpha-galactosidase a grown in a cell culture medium having non-precipitating phosphate; contacting said lysate with a first chromatography media that binds α-d-mannopyranosyl or α-d-glucopyranosyl; eluting alpha-galactosidase a from the first chromatography media to generate a first eluate having alpha-galactosidase a, wherein said eluting includes at least one elution pause between 4 and 16 hours; contacting the first eluate with a second chromatography media that binds galactose binding proteins; and eluting alpha-galactosidase a from said second chromatography media to generate a second eluate containing said recombinant alpha-galactosidase a..
Research Foundation Of The City University Of New York
Flame-retardant agent composition and flame-retardant resin composition containing same, and molded article
Wherein r1, r2 and r3 are as defined in the general formula (i) is 1.8% by area or less when the organophosphorous compound is determined by gel permeation chromatography (gpc).. .
Purification vitamin k dependent proteins by anion exchange chromatography
The present invention relates to a method for the purification of vitamin k dependent proteins with high yield and high purity, particularly enriched in active protein, on anion exchange resin materials, to vitamin k dependent proteins obtainable by said method, and to a kit comprising means for carrying out said method.. .
Porous composite particulate materials, methods of making and using same, and related apparatuses
In an embodiment, a porous composite particulate material includes a plurality of composite particles. Each composite particle includes an acid-base-resistant core particle at least partially surrounded by one or more layers of acid-base-resistant shell particles.
Us Synthetic Corporation
Apparatus and methods for controlling the temperature of a chromatography column
An apparatus for controlling the temperature of a chromatography column includes a thermal-isolation vessel; a heater in thermal communication with the chromatography column and disposed on the thermal-isolation vessel; a temperature sensor disposed to directly measure the temperature of the chromatography column; and a control unit in signal communication with the temperature sensor to control the heater in response to light the direct measurement.. .
Waters Technologies Corporation
Method for analyzing samples of a biological fluid and performing the same
The present invention relates to methods for continuous or near-continuous separation and purification of samples, particularly biological samples, to reduce the number or volume of non-targeted analytes in those samples to enable improved mass spectrometric analysis of analytes of interest, and apparatuses for conducting those methods, utilizing a transport agent with a core domain and a binding domain, the transport agent exceeding 200 kilodaltons or 10 nm, and the binding domain targeted to the analytes of interest, in conjunction with size-exclusion based chromatography to separate the transport agent-analyte of interest complex from non-targeted analytes that are not bound, or are only non-specifically bound, to the transport agent.. .
Gaseous mercury detection systems, calibration systems, and related methods
Embodiments disclosed herein are directed to gaseous mercury detection systems, calibration systems, and related methods. The gaseous mercury detection systems are configured to detect gas-phase mercury-compounds present in ambient air.
Utah State University
Multi-path selector valve
A multi-path selector valve used in liquid chromatography and other analytical methods for directing fluid along alternate paths of a flowstream. The selector valve has a stator and a rotor.
Idex Health & Science Llc
Thermoplastic elastomer composition and molded article thereof
The thermoplastic elastomer composition includes (a) a polypropylene resin, (b) a non-conjugated diene copolymer having a mooney viscosity (ml1+4, 125° c.) of 40 to 85 and a molecular weight distribution of 1.5 to 3.0 and whose eluted amount at −15° c. Measured by cross-fractionation chromatography is 50% to 99.9% of the overall eluted amount, and (c) an ethylene copolymer having a mooney viscosity (ml1+4, 125° c.) of 20 to 30 and a density of 0.850 g/cm3 to 0.890 g/cm3.
Sumitomo Chemical Company, Limited
Method and system for decoupling a capillary column from a gas chromatography-mass spectrometry (gc-ms) system
A column-sealing tool is provided for use during decoupling of a capillary column from a gas chromatography-mass spectrometry (cg-ms) system. The column-sealing tool includes an engagement structure for removably securing the column-sealing tool within a high vacuum enclosure of the gc-ms system, at a location between a high vacuum end of a transfer line and a mass analyzer of the gc-ms system.
Thermo Finnigan Llc
Toxoid, compositions and related methods
Methods of producing purified clostridial toxin comprising tangential flow filtration, hydrophobic interaction chromatography and anion exchange chromatography are disclosed. These methods provide good yields of c.
Sanofi Pasteur, Inc.
Fkbp52 targeting agent pharmaceutical compositions
Liposomes comprising an fkbp52 targeting agent (fta) are disclosed. Pharmaceutical compositions comprising an fta, a solvent, and a surfactant are disclosed.
The Board Of Regents Of The University Of Texas System
Chromatography/mass spectrometry data processing device
Using the intensity ratio of peaks on a standard mass spectrum of the target compound and peaks with the same m/z on the measured mass spectrum near the retention time of said compound, a scale factor waveform close to the chromatogram shape based on the target compound alone is computed, and m/z candidates for quantitation are extracted based on the correlation between the measured mass chromatogram of the target compound and the scale factor waveform. Furthermore, an intensity ratio is determined with reference to the m/z peaks showing the greatest scale factor on the measured mass spectrum at the measurement time point showing the greatest scale factor in the scale factor waveform, and m/z candidates for quantitation/confirmation are narrowed down based on whether they fall within an allowable range based on peak intensity ratio on the standard mass spectrum..
Methods for gas-phase thermochromatographic separations of fission and activation products
Methods are provided for characterizing samples containing chemical elements such as rare earth elements, actinides, and heavy transition metals by treating the samples to form volatile complexes of the elements (e.g., β-diketonate complexes or other organic ligand complexes of the elements) and then analyzing the complexes, for example, via gas-phase thermochromatography. Also provided are methods for separating and/or recovering such chemical elements.
University Of Tennessee Research Foundation
Method for preparing a concentrate of factor xi
The invention concerns a concentrate of human factor xi having high specific activity prepared using a method comprising a filtration-adsorption step and a chromatography step on cation exchange resin. The concentrate obtained is fully adapted for therapeutic use as substitution therapy in cases of factor xi deficiency..
Laboratoire Francais Du Fractionnement Et Des Biotechnologies
Polymer films having improved heat sealing properties
A polymer composition comprising an ethylene alpha-olefin copolymer, wherein the polymer composition is characterized as having (a) a density in the range of from greater than about 0.910 g/cc to about 0.930 g/cc, as determined according to astm d1505; (b) a melt index in the range of from greater than about 0.5 g/10 min to about 3 g/10 min, as determined by astm d1238, condition 190° c./2.16 kg; (c) a molecular weight distribution of from about 3.4 to about 12, as determined by gel permeation chromatography (gpc); (d) a weight average molecular weight of from greater than about 85 kg/mol to about 160 kg/mol, as determined by gel permeation chromatography (gpc); and (e) a z-average molecular weight of from greater than about 210 kg/mol to about 500 kg/mol, as determined by gel permeation chromatography (gpc).. .
Chevron Phillips Chemical Company Lp
Purification of vwf
The present invention provides a method of purifying multimeric von willebrand factor (vwf) from a solution comprising multimeric vwf and contaminants. The method comprises passing the solution through a chromatography column comprising beads of a mixed mode chromatography resin coated with a size-exclusion inactive shell and collecting the multimeric vwf which passes through the column without binding to the resin..
Purification platform for bispecific antibodies
High resolution protein a chromatography employing a chaotropic agent and ph gradient or ph step elution buffer results in improved peak resolution between closely related molecular species. Bispecific antibodies containing a protein a-binding-ablating substitution ch3 domain paired with a protein a-binding ch3 domain are separated with high peak resolution from monospecific antibodies containing a protein a-binding-ablating substituted ch3 domain paired with the protein a-binding-ablating substituted ch3 domain and monospecific antibodies containing a protein a-binding ch3 domain paired with the protein a-binding ch3 domain.
Regeneron Pharmaceuticals, Inc.
Methods for purifying antibodies
A method for purifying a protein comprising an antibody, antibody fragment, or immunoglobulin single variable domain, from a solution containing at least one contaminant by superantigen chromatography comprising: a) adsorbing the protein to the superantigen immobilized on a solid support; b) removing the at least one contaminant by contacting the immobilized superantigen containing the adsorbed protein with a first wash buffer comprising an aliphatic carboxylate; and c) eluting the protein from the superantigen immobilized on the solid support.. .
Glaxosmithkline Intellectual Property (no.2) Limited
Hydrophobic interaction protein chromatography under no-salt conditions
Various aspects and embodiments of the present disclosure relate to the purification antibodies by hydrophobic interaction chromatography under no-salt conditions.. .
Biogen Ma Inc.
Removal of leaked affinity purification ligand
The invention provides for the removal of a large fraction of contaminants from protein preparations while maintaining a high level of recovery using tentacle anion exchange matrix chromatography medium. Using the methods of the invention, leached affinity chromatography contaminants can be removed from recombinant protein preparations..
Ion exchange purification of mrna
The current landscape for preparative chromatographic rna purification uses reversed phase hplc, but this technique presents many issues with process scale up and ion exchange for preparative purification has only been used for short rnas. The invention provides preparative purification of rna (e.g., mrna) using ion (e.g., anion) exchange chromatography that allows for separation of longer rnas up to 10,000 nucleotides in length via a scalable method.
Modema Therapeutics, Inc.
Rna purification methods
Methods for purifying rna from a sample, comprising one or more steps of tangential flow filtration, hydroxyapatite chromatography, core bead flow-through chromatography, or any combinations thereof. These techniques are useful individually, but show very high efficiency when used in combination, or when performed in particular orders.
Glaxosmithkline Biologicals Sa
Sample plate for maldi-tof mass spectrometer and manufacturing the sample plate and mass spectrometry method using the sample plate
The present invention relates to a sample plate to be used for a maldi-tof (matrix assisted laser desorption ionization time of flight) mass spectrometer, and more particularly, to a sample plate for a maldi-tof mass spectrometer, which is particularly useful for molecular weight measurement of a high-volatile material, a method of manufacturing the sample plate and a mass spectrometry method using the sample plate. Object of the present invention to provide a method capable of performing a mass spectrometry for a high-volatile material by using a maldi-tof mass spectrometer so as to overcome the limits of the gas chromatography method of the related art.
Industry-academic Cooperation Foundation, Yonsei University
Improved mapping glycans of glycoproteins
The use of anthranilic acid (2-aa) to label n-glycans prior to separation using a reversed-phase liquid chromatography (rp-lc) column under acidic conditions using formic acid is described herein. Negatively charged 2-aa offers stronger retention on the reversed phase column than 2-aminobenzamide (2-ab) in rp-lc and allows efficient ionization and detection of 2-aa labeled n-glycans.
Positioning guides and ion sources
Certain embodiments described herein are directed to inserts and ion sources. In some examples, an insert can comprise a positioning guide configured to provide for visual positioning of a chromatography column under the positioning guide.
Perkinelmer Health Sciences, Inc.
Matching thermally modulated variable restrictors to chromatography separation columns
Thermally modulated variable restrictors used in chromatography systems enable independent control of system pressure and linear velocity of a compressible mobile phase passing through a chromatography column. A method for configuring a chromatography system with independent control of system pressure and mass flow rate of a compressible mobile phase includes determining a type of chromatography separation column to be used in the chromatography system, matching a thermally modulated variable restrictor to the type of chromatography separation column for use together during operation of the chromatography system, and bundling the chromatography column with its matching thermally modulated variable restrictor for distribution as a single package..
Waters Technologies Corporation
Thermally modulated variable restrictor
Thermally modulated variable restrictors used in chromatography systems enable independent control of system pressure and linear velocity of a compressible mobile phase passing through a chromatographic column. The restrictors include a restrictor body having a fluidic channel with an inlet that receives the mobile phase from the column and an outlet through which the mobile phase leaves the fluidic channel.
Waters Technologies Corporation
Functionalized metal oxides as a stationary phase and a surface template for micro gas chromatography separation columns
The present invention provides a detector and method for detecting substances in complex mixtures. The detector includes a microfabricated preconcentrator, a separation column with an on-chip thermal conductivity detector, a controller for controlling flow and thermal management and a user interface.
Monitoring a dynamic system by liquid chromatography-mass spectrometry
The present invention provides a method for monitoring of profile changes of components in a dynamic system such as a cell-free in vitro protein synthesis system by using liquid chromatography (lc) combined with mass spectrometry (ms). In an additional aspect, this invention provides a method for enhancing the yield and/or reproducibility in a cell-free protein synthesis system by modulating the level and/or activity of a protein component that has regulatory effects on the system..
Sutro Biopharma, Inc.
Analysis of mrna heterogeneity and stability
Reversed phase-high performance (high pressure) liquid chromatography (rp-hplc) and size exclusion chromatography (sec) methods have been developed for monitoring structural and size heterogeneity as well as stability of large rna transcripts, including lengths of up to at least 10,000 nucleotides. The methods are designed for significantly larger mrnas that could be monitored in the past, including lengths of up to at least 10,000 nucleotides, and including chemically modified rna transcripts.
Moderna Therapeutics, Inc.
Devices and methods for purification, detection and use of biological cells
This invention relates to devices and methods for purifying, detecting and using biological cells. A variety of cell types including viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column.
Curable resin composition and cured product thereof, encapsulant, and semiconductor device
The curable resin composition according to the present invention includes a polyorganosiloxane (a), an isocyanurate compound (b), and a silane coupling agent (c). The polyorganosiloxane (a) is an aryl-containing polyorganosiloxane.
Polycarbonate-polyorganosiloxane copolymer and producing same
Provided is a polycarbonate-polyorganosiloxane copolymer, including a specific polycarbonate block unit (a) and a specific polyorganosiloxane block unit (b), in which in a differential molecular weight distribution curve obtained from measurement of the polyorganosiloxane block unit (b) by gel permeation chromatography using the polystyrene calibration curve, the curve having an axis of abscissa indicating a logarithmic value log(m) of a molecular weight m and an axis of ordinate indicating dw/d log(m) obtained by differentiating a concentration fraction w with respect to the logarithmic value log(m) of the molecular weight, (1) a dw/d log(m) value becomes maximum in a range of 3.4≦log(m)≦4.0, and (2) in the differential molecular weight distribution curve, a ratio of a value obtained by integrating the dw/d log(m) value over a range of 4.0≦log(m)≦4.5 to a value obtained by integrating the dw/d log(m) value over the entire range of the log(m) is 6% or more and 40% or less.. .
Idemitsu Kosan Co., Ltd.
Simulated moving bed chromatographic separation process
The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (pufa) product, from a feed mixture, which process comprises introducing the feed mixture to a simulated or actual moving bed chromatography apparatus having a plurality of linked chromatography columns containing, as eluent, an aqueous alcohol, wherein the apparatus has a plurality of zones comprising at least a first zone and a second zone, each zone having an extract stream and a raffinate stream from which liquid can be collected from said plurality of linked chromatography columns, and wherein (a) a raffinate stream containing the pufa product together with more polar components is collected from a column in the first zone and introduced to a nonadjacent column in the second zone, and/or (b) an extract stream containing the pufa product together with less polar components is collected from a column in the second zone and introduced to a nonadjacent column in the first zone, said pufa product being separated from different components of the feed mixture in each zone.. .
Basf Pharma (callanish) Limited
Block copolymer and production the same
A block comprised of a copolymer is obtained by ring-opening polymerization of a cyclic polyarylene sulfide, so that a block copolymer is produced to have a maximum peak molecular weight measured by size exclusion chromatography (sec) in a range of not less than 2,000 and less than 2,000,000 and have a unimodal molecular weight distribution in this range.. .
Toray Industries, Inc.
Chromatographic purification of antibodies from chromatin-deficient cell culture harvests
Methods for the improved purification of antibodies and other proteins from protein preparations including the steps of conditioning the protein preparation by contacting it with multivalent organic ions, then applying the conditioned preparation to an adsorptive chromatography medium.. .
Agency For Science, Technology And Research
Provided is a coffee beverage, including the following components (a), (b), and (c): (a) at least one selected from the group consisting of catechol and pyrogallol; (b) 3,4-dicaffeoyl-1,5-quinolactone; and (c) chlorogenic acids, in which a ratio of a total peak area of the component (a) to a peak area of the component (b), ((a)/(b)), as determined by liquid chromatography-time of flight mass spectrometry (lc-tof/ms) of the coffee beverage, is from 0 to 2.0, and in which a content of the component (c) in the coffee beverage is from 0.05 to 2% by mass.. .
Method for detection of adenosine and metabolites thereof
This invention relates is a label-free, enzyme-free, aptamer-free method for simultaneously measuring adenosine, and its intracellular metabolites, e.g., amp, adp and atp, using high pressure liquid chromatography coupled to electrochemical detector (hplc-ecd).. .
St. Jude Children's Research Hospital
Uv light emitting diode as light source in gas chromatography-uv absorption spectrophotometry
An apparatus for analyzing a sample, including a sample receiving device, a gas chromatograph and a spectrophotometer, said spectrophotometer including a uv light emitting diode as the light source, an elongated chamber and a detector. The uv light source is arranged to illuminate sample substances conducted through the chamber, and the detector is arranged to identify sample substances by uv absorption spectroscopy..
Method and system for monitoring biomolecule separations by mass spectrometry
Monitoring biomolecule separations by mass spectrometry is described. The mixture to be separated is introduced into a first fluidic stream, which then passes through a chromatography column.
Microsaic Systems Plc
Method to identify bacterial species by means of gas chromatography/mass spectrometry in biological samples
Method to identify bacterial classes in a biological sample, in particular a urine sample, that provides to carry out an analysis by means of gas chromatography-mass spectrometry (gc/ms) of the volatile components, such as metabolites and catabolites, of the sample in order to identify a graphic plot characteristic of a specific bacterial class.. .
Alifax Holding Spa
A paving binder according to the present invention is used for a paving mixture for forming a pavement, including a polyamide resin having a weight average molecular weight, as measured by gel permeation chromatography of 2,000 or more and 10,000 or less and having an area, corresponding to a component having a molecular weight of 20,000 or more and 500,000 or less, of 0.5% or more and 12% or less of a total area in a chromatogram of molecular weight distribution as measured by gel permeation chromatography. Thus, tolerance for a severe temperature condition or loading condition can be improved, while ensuring convenience from the standpoint of laying such that the laying can be made by the conventional machine composition or laying system..
Materials and methods for removing endotoxins from protein preparations
A method includes (i) adding allantoin in a supersaturating amount to a protein preparation including a desired protein and at least one endotoxin as a contaminant, (ii) removing solids after the adding step to provide a sample for further purification by void exclusion chromatography on a packed particle bed of electropositive particles in a column, the packed particle bed having an interparticle volume, (iii) applying a sample volume to the packed particle bed, wherein the electropositive particles support void exclusion chromatography, and wherein the sample volume is not greater than the interparticle volume, and (iv) eluting a purified sample including the desired protein and a reduced amount of the endotoxin. The method is optionally carried out with only the allantoin treatment or only the void exclusion chromatography..
Agency For Science, Technology And Research
Method of purifying an antibody
A method of purifying an antibody composition comprises application of anion exchange chromatography late in the purification process. An ultrafiltration/diafiltration-purified antibody composition is subjected to anion exchange chromatography (aex) to form a pharmaceutically-pure antibody composition..
Synthon Biopharmaceuticals B.v.
Mobile phase degassing for nano-flow liquid chromatography
Systems and methods for degassing liquids in nano-flow liquid applications (fig. 3).
The Translational Genomics Research Institute
Functionalised triblock copolymers and compositions containing such polymers
Amphiphilic triblock copolymers b-a-b, wherein a is a linear poly(ethylene glycol) block, having a number average molecular weight (mn) of between 900 and 3000 daltons, determined with size exclusion chromatography; wherein b are hydrophobic blocks with at least two cyclic monomers selected from the group consisting of glycolide, lactide, 1,3-dioxan-2-one, 5,5-dimethyl-1,3-dioxan-2-one, 1,4-dioxan-2-one, 1,4-dioxepan-2-one, 1,5-dioxepan-2-one, each b-block having a number average molecular weight (mn) of between 400 and 2000 daltons, determined with size exclusion chromatography; and wherein 25% to 100% of the polymer hydroxyl end-groups are covalently modified with at least one derivative of a c2-c20 fatty acid. The invention also relates to compositions with such polymers and the use thereof..
Ingell Technologies Holding B.v.
Purification of nanoparticle-antibody conjugates
Methods of purifying antibody-nanoparticle conjugates with size exclusion chromatography are provided.. .
Bio-rad Laboratories, Inc.
Thin layer chromatography plates and related methods
In an embodiment, a method for manufacturing a thin layer chromatography (“tlc”) plate is disclosed. The method includes forming a layer of elongated nanostructures (e.g., carbon nanotubes), and at least partially coating the elongated nanostructures with a coating.
Brigham Young University
Measurement method, measurement apparatus, and eluent
Provided is a method of measuring at least one kind of hemoglobin, selected from abnormal hemoglobin, or hemoglobin that is a marker for thalassemia, the method comprising: performing high-performance liquid chromatography, using: an eluent l that contains a phosphoric acid monohydrogen dialkali metal salt (component 1) and a phosphoric acid dihydrogen monoalkali metal salt (component 2) as components, and that has a content of component 1 of from 0.08% by mass to 0.50% by mass with respect to a total mass of the eluent l, a content of component 2 of from 0.04% by mass to 1.2% by mass with respect to a total mass of the eluent l, and a ratio of component 2/component 1 of from 0.4 to 10.. .
Fully automated analytical determination of chloroethers in water and urine sample
A fully automated flow assisted-solid-phase microextraction (fa-spme) is developed for the determination of chloroethers in aqueous samples. A ctc combipal autosampler coupled with gas chromatography-mass spectrometry (gc-ms) is used to automate the extraction process.
King Abdulaziz City For Science And Technology
Determination of polycyclic aromatic hydrocarbons in water using nanoporous material prepared from waste avian egg shell
Nanoporous polymorphic crystals of caco3 were used as sorbent and were applied in the dispersive micro-solid-phase extraction of selected polycyclic aromatic hydrocarbons as target analytes. After separation of the analytes on gas chromatography, they were successfully quantified with external calibration using flame ionization detection.
King Fahd University Of Petroleum And Minerals
Porous material, producing method thereof, and serial producing apparatus thereof
Provided is a porous material made of at least an aliphatic polyester resin or an aliphatic polycarbonate resin, and having a porosity of 70% or higher, wherein a polystyrene equivalent weight average molecular weight of the resin measured by gel permeation chromatography is 300,000 or greater.. .
Ricoh Company, Ltd.
Disposable tip for pricking septum of a sample vial for sample preparation and/or analysis.
This invention relates to a disposable pipette tip or tube designed to perform biological or chemical sample preparation, purification or separation where the samples are in a closed bottle with a lid with septum or membrane. In an automatic sampler for hplc or gas chromatography, septum closed vials are used to prevent samples from drying out or evaporating during the analytical process.