This page is updated frequently with new Chromatography-related patent applications.
| Compositions and methods for analysis of protein sequences and post-translational modifications|
The application discloses, compositions, methods, systems, and apparatuses for rapid sequence analysis of proteins, including location of post-translational modifications and disulfide bonds, is described. Limited digestion of fully denatured antibody occurs in seconds by flowing sample in 8 m urea at constant pressure through a micro column reactor containing immobilized aspergillopepsin i, resulting in a product mixture containing 3-10 kda peptides, which is then fractionated by capillary column chromatography and analyzed by both electron transfer dissociation (etd) and collision activated dissociation mass spectrometry.
University Of Virginia Patent Foundation
| Method for measuring human exhaled air|
A method for measuring human exhaled air by means of gas chromatography and ion mobility spectrometry, wherein an exhaled air sample enters a sample loop via a sample inlet and a multi-port valve and is subsequently conveyed by means of a carrier gas from the sample loop, via the multi-port valve through a gas chromatographic column, into an ion mobility spectrometer, and measured, which method is to provide reliable and accurate measurement results. This object is achieved in that the following steps are carried out before an exhaled air sample is introduced into the sample loop: (a) first flushing at least the gas chromatographic column, the ion mobility spectrometer and the sample loop with a flushing gas and then switching the multi-port valve in such a way that the flushing gas enters the ion mobility spectrometer and is measured; (b) then stopping the supply of flushing gas and switching the multi-port valve in such a way that ambient air flows through the gas chromatographic column into the ion mobility spectrometer and is measured; (c) then flushing at least the gas chromatographic column, the ion mobility spectrometer and the sample loop with humidified flushing gas and switching the multi-port valve in such a way that the humidified flushing gas enters the ion mobility spectrometer and is measured; and (d) subsequently stopping the supply of humidified flushing gas, conducting an exhaled air sample into the sample loop, conveying the sample, by means of the carrier gas, through the gas chromatographic column into the ion mobility spectrometer, and measuring the sample..
Imspex Diagnostics Ltd
| Methods for mass spectrometric quantitation of analytes extracted from a microsampling device|
Mass spectrometric methods are described for determining the amount of analyte in a sample collected by a microsampling device. Provided herein are methods directed to quantitating the amount of an analyte in a sample by extracting an analyte from a sample collected by a microsampling device, purifying the sample by liquid chromatography, ionizing the analyte to generate one or more ions detectable by mass spectrometry; and determining the amount of the one or more ions by mass spectrometry.
Quest Diagnostics Investments Llc
| A bioreactor arrangement and continuous process for producing and capturing a biopolymer|
The present invention relates to a bioreactor arrangement for producing a biopolymer expressed by a cell and a continuous process for a capturing the biopolymer employing two chromatography units operated in series or independently.. .
Cmc Biologics A/s
| Method for separating hyaluronan and quantifying its molecular mass distribution in biological samples|
The present invention provides a facile method for separation (fractionation) of ha in a sample over a broad m range, including low m ha, by ion exchange (iex) chromatography. The present invention also provides an assay method for quantifying in a sample the presence of low m ha in total ha isolated from a biological source.
New York University
| Affinity ligands and methods relating thereto|
Affinity ligands useful for mild elution affinity chromatography, including affinity ligands specific for immunoglobulins m, a, and e, are disclosed as are method of identifying and using such affinity ligands.. .
Bio-rad Laboratories, Inc.
| A chromatography capturing a biopolymer|
The present invention relates to a chromatography system (20) wherein the chromatography system comprises an eluting system (10) and a capturing system (11) consisting of at least two chromatography units (2,3) operated alone or in series and a capturing process employing in-line buffer dilution in, which concentrated buffers are blended with water and provided to the chromatography units.. .
Cmc Biologics A/s
| Process for producing and purifying factor viii and its derivatives|
Disclosed is a method for producing proteins having factor viii procoagulant activity in serum-free medium by in vitro culturing of mammalian cells, wherein the serum-free medium contains an inhibitor against the protease released from cultured cells. In accordance with this invention, the inhibitor can protect the cleavage of a target protein during cultivation and increase homogeneity of a target molecule, wherein the inhibitor can be a dextran sulfate.
Sk Chemicals Co., Ltd.
| Use of cation-exchange chromatography in the flow-through mode to enrich post-translational modifications|
The present invention relates to improved methods in the separation recombinant polypeptides with post-translational modifications from complex mixtures through the use of a cation exchange medium.. .
Biogen Ma Inc.
|Thermal conductivity detector and detector module|
A thermal conductivity detector for a gas chromatograph includes a heatable resistive detector element configured to be physically arranged in a flow of analytes eluting from a chromatography column and electrically arranged together with resistors in separate arms of a measuring bridge, wherein to provide a new configuration of the thermal conductivity detector to allow high detector sensitivity and to meet intrinsic safety requirements, the detector element includes at least two equal detector sub-elements that are configured to be physically arranged in series in the flow of analytes and electrically arranged in parallel with each other, where the detector element in one arm and a reference resistor in the other arm of the same half of the measuring bridge are configured such that the total resistance of the parallel detector sub-elements at operating temperature is at least approximately equal to the resistance of the reference resistor.. .
A process for manufacturing factor viii having an improved ratio of fviii:c/fviii:ag
A process for manufacturing of a factor viii product having a ratio of fviii:c/fviii:ag of at least 0.7 in the factor viii product by using chromatography wherein at least one chromatographic step is performed by means of employing; an affinity chromatography resin having an affinity for specifically binding of factor viii which is effected by an affinity ligand which is immobilised on the affinity chromatography resin, said affinity ligand is a 13 kd yeast derived fab antibody fragment directed to the factor viii molecule. An anionic chromatography resin.
Method for purifying reduced form of beta-nicotinamide adenine dinucleotide
The present invention discloses a method for purifying reduced form of β-nicotinamide adenine dinucleotide, comprising the steps of: sequentially microfiltrating and nanofiltrating a reaction solution obtained after an enzymatic reaction, to collect a concentrate for use; then adding an ion pair reagent to the concentrate, and purifying by gradient elution using a reverse-phase chromatographic column as a stationary phase, a buffer solution as a phase a, and ethanol as a phase b; changing the cations in the purified filtrate into sodium ions by using a cation exchange resin; and nanofiltrating the filtrate obtained in step c, and finally freeze drying it in a vacuum freeze drier. In the present invention, the reduced form of β-nicotinamide adenine dinucleotide is purified by reverse phase high performance liquid chromatography and cation exchange, such that the reduced form of β-nicotinamide adenine dinucleotide has a high purity and high yield, thus meeting the requirements in industry..
Bontac Bio-engineering (shenzhen) Co., Ltd
Method for purifying oxidized form of beta-nicotinamide adenine dinucleotide
The present invention discloses a method for purifying oxidized form of β-nicotinamide adenine dinucleotide, comprising the steps of: sequentially microfiltrating and nanofiltrating a reaction solution obtained after an enzymatic reaction by using filteration membranes, to collect a concentrate for use; then adding an acid to the concentrated filtrate, to adjust the ph of the concentrate, and purifying by gradient elution using a reverse-phase chromatographic column as a stationary phase, a buffer solution as a phase a, and ethanol as a phase b; and concentrating the purified solution by nanofiltrating it with a filtration membrance, and then freeze drying it in a vacuum freeze drier. In the present invention, the oxidized form of β-nicotinamide adenine dinucleotide is purified by reverse phase high performance liquid chromatography, such that the oxidized form of β-nicotinamide adenine dinucleotide has a high purity and high yield, thus meeting the requirements in industry..
Bontac Bio-engineering (shenzhen) Co., Ltd
Toner, developer using the toner, image forming apparatus
A toner including at least a crystalline resin as a binder resin, wherein a tetrahydrofuran soluble content of the toner includes 5.0% or more as a peak area of a component having a molecular weight of 100,000 or greater in a molecular weight distribution measured by gel permeation chromatography, and the tetrahydrofuran soluble content of the toner has a weight-average molecular weight of 20,000 to 60,000.. .
Method and thermal conductivity detector
A thermal conductivity detector includes a heatable resistive detector configured to be physically arranged in an analytes flow eluting from a chromatography column and electrically arranged with resistors in separate arms of a measuring bridge, an amplifier which detects differential voltage between two opposite nodes of the bridge and applies an output voltage to other opposite nodes of the measuring bridge to maintain the detector at a constant operating temperature, and an additional resistor with a controllable switch in parallel connected in series with the detector or resistor arranged in one arm of the bridge, where the switch is periodically turned on and off at a predetermined duty cycle and/or controlled by information on characteristic times-of-arrival of analytes at the detector to compensate for operating temperature uncertainties due to manufacturing variations of the resistors and/or to allow for processing small and large peaks of a chromatogram with highest available resolution.. .
Method of purifying monoclonal antibodies
A new platform method to purify plant-based monoclonal antibodies is provided. Such a method includes an antibody purification platform that involves a standardized procedure for the production of a wide array of different antibodies within a simplified context.
Kentucky Bioprocessing, Inc.
Purification of erythropoietin
In the present invention a method for purifying erythropoietin comprising at least one chromatography step using a stationary phase containing hydroxyapatite is reported. The method comprises the following steps i) the erythropoietin in a solution containing calcium-ions is brought into contact with a stationary phase containing hydroxyapatite equilibrated with a solution containing calcium-ions and namely under conditions under which the erythropoietin binds to the stationary phase containing hydroxyapatite, ii) a solution is passed over the stationary phase containing hydroxyapatite from i) which contains less calcium-ions than the previous solution and the erythropoietin is not detached from stationary phase containing hydroxyapatite, and iii) a further solution which contains less than 0.5 mm calcium-ions is passed over the stationary phase containing hydroxyapatite from ii) whereby the erythropoietin is detached from the stationary phase containing hydroxyapatite..
F. Hoffmann-la Roche Ag
Affinity chromatography media and chromatography devices
chromatography media and devices containing chromatography media are disclosed. Methods of making chromatography devices and methods of using chromatography devices containing the chromatography media are also disclosed..
Flash chromatography cartridge
A low pressure liquid chromatographic cartridge has a tubular polymer container that receives a chromatographic packing material. The container has an outlet port located at its downstream end and container threads formed on its upstream end.
Scientific Plastics Products, Inc.
Novel pure substance extracting method
The present invention provides a novel pure substance extracting method. Differing from conventional pure substance extracting method utilizing chromatography technique to treat a red mold product with an extracting and purifying process by using a plurality of organic extracting solution, the present invention merely utilizes water and ethanol solution to extract the pure substances of monascin and ankaflavin from the red mold product.
Sunway Biotech Co., Ltd
Purification of herpes virus
The present disclosure provides a method to prepare purified enveloped viral particle preparations employing ion exchange chromatography and tangential flow filtration.. .
Sanofi Pasteur Biologics, Llc
Liquid chromatography-mass spectrometry device
This invention improves the sensitivity of a liquid chromatography-mass spectrometry device by reducing the number of neutral particles that are not ionized during ionization and the number of low-molecular ions from a solvent used in the liquid chromatography. Said liquid chromatography-mass spectrometry device is provided with ion sources, a mass spectrometry unit, a detector, and three electrodes laid out so as to be parallel to each other.
Hitachi Hich-technologies Corporation
Method for purifying antibody having low isoelectric point
The present inventors discovered that additional aggregation of low-pi antibody can be suppressed by removing formed antibody aggregates after a certain period of time following protein a column purification, acidic treatment, and neutralization. Furthermore, the present inventors found that efficient impurities removal for a low-pi antibody can be accomplished by using an anion exchange resin in the bind/elute mode and then a hydrophobic interaction chromatography or multimodal chromatography resin, compared with conventional methods..
Chugai Seiyaku Kabushiki Kaisha
Hydrophobic monomers, hydrophobically-derivatized supports, and methods of making and using the same
Wherein n is an integer of 0 or 1; r1 is independently selected from at least one of: a hydrogen atom, alkyls, aryls, and alkylaryls, wherein the alkyls, aryls, and alkylaryls have a total of 10 carbon atoms or less; r3 is a hydrophobic group selected from at least one of: alkyls, aryls, alkylaryls and ethers, wherein the alkyls, aryls, alkylaryls and ethers have a total number of carbon atoms ranging from 4 to 30; r4 is h or ch3; x is o or nh. In some embodiments the hydrophobic monomer is derived from an amine or an alcohol (hxr3) that has a hydrophilicity index of 25 or less.
Sterilizing chromatography columns
If one sterilizes pre-packed, plastic chromatography columns with an appropriate level of gamma irradiation, the resulting sterile chromatography columns maintain sufficient packing media function and maintain column mechanical properties and pressure ratings.. .
Method for purifying cys-linked antibody-drug conjugates
The present invention relates to a method for purifying a mixture of cysteine-linked antibody-drug conjugates, wherein the amount of non-conjugated antibody is in the range of 0-40% by weight, using hydrophobic interaction chromatography (hic). The mixture is loaded onto a preparative hic column using a 0.2-1.5 m aqueous salt solution, in which non-conjugated antibody is collected in a flow-through fraction, followed by elution of a purified mixture of cysteine-linked antibody-drug conjugates using a 0-100 mm aqueous salt solution..
Synthon Biopharmaceuticals B.v.
Containers for chromatography media
The invention relates to containers or bags for chromatographic media and methods of packing chromatography columns using such containers. The bags may be used for storing and/or transporting chromatographic media and can be inserted directly into the chamber of a chromatography column in readiness for use..
Ge Healthcare Bioprocess Ab
Composite polyamide membrane having high acid content and low azo content
A thin film composite polyamide membrane comprising a porous support and a thin film polyamide layer characterized by possessing: i) an azo (—n═n—) content of from 0.30% to 0.80%, as measured by pyrolysis gas chromatography; and ii) a dissociated carboxylate content of at least 0.18 mol/kg as measured by rbs at ph 9.5.. .
Dow Global Technologies Llc
Chromatographic device and isolating and purifying nucleic acids
The present invention relates to a chromatographic device for isolating and purifying nucleic acids, preferably genomic dna, by gel filtration chromatography, a method for isolating and purifying nucleic acids, preferably genomic dna, using this device and a kit comprising this device.. .
A rotary valve comprising a stator and a rotor, wherein the stator comprises at least three primary connection ports and at least three secondary connection ports, and wherein rotor interconnection paths are arranged to in the different rotor positions interconnect the primary connection ports with the secondary connection ports such that all of at least three secondary connection ports can be connected one at the time to each of at least three primary connection port by rotating the rotor into the different rotor positions. A chromatography system comprising at least three chromatography columns and a column inlet rotary valve, a column outlet rotary valve and a feed recirculation flow path..
Ge Healthcare Bio-science Ab
Methods of polynucleotide preparation using multivalent cation salt compositions
Aspects of the disclosure include methods for the preparation of a polynucleotide. In some embodiments, the method includes contacting a first polynucleotide composition including: a polynucleotide having a sequence of 7 or more nucleoside subunits and at least two of the nucleoside subunits are joined by a n3′→p5′ thiophosphoramidate inter-subunit linkage; and non-target synthetic products and reagents; with a multivalent cation salt to precipitate a polynucleotide salt including at least one multivalent cation counterion; and separating the polynucleotide salt from the contacted first polynucleotide composition to produce a second polynucleotide composition including the polynucleotide salt.
Composite polyamide membrane having azo content and high acid content
A thin film composite polyamide membrane comprising a porous support and a thin film polyamide layer characterized by possessing: i) an azo (—n═n—) content of from 0.40% to 1.00%, as measured by pyrolysis gas chromatography; and ii) a dissociated carboxylate content of at least 0.40 mol/kg as measured by rbs at ph 9.5.. .
Dow Global Technologies Llc
A chromatography system and method
A chromatography system comprising at least two chromatography columns, where a feed recirculation of outflow from a primary load column to the inlet of a secondary load column is combined such that the feed recirculation outflow from all columns that will be used as primary load columns in the system will pass through one and the same feed recirculation flow path.. .
Ge Healthcare Bio-sciences Ab
Purification of bacterial capsular polysaccharide by two-phase aqueous micellar system
The present invention discloses a method for purification of bacterial capsular polysaccharide at low temperature (<20° c.) by salt-induced two-phase aqueous micellar system with tritonx-114 and can significantly reduce the endotoxins. Impurity proteins and the endotoxins need to be removed as many as possible in the preparation process of bacterial capsular polysaccharide vaccine to reduce the side reaction of the vaccines.
Institute Of Medical Biology Chinese Academy Of Medical Sciences & Peking Union Medical College
Protein tyrosine phosphatase inhibitor, preparation method and uses thereof
Disclosed in the present invention is a protein tyrosine phosphatase inhibitor. The preparation method therefor is: extracting the crude product from the isaria fumosorosea wize solid or liquid fermentation broth using ethyl acetate, ethanol, methanol, or a mixed solvent of chloroform and methanol; separating the obtained extract using column chromatography on silica gel; and obtaining the target product.
Betacryptoxanthin compositions, processes for preparation and uses thereof
Compositions are described that include a betacryptoxanthin extract, rich in trans-betacryptoxanthin, which are prepared by a cost and time effective process and are for use in methods of enhancing cardio-respiratory fitness for physical performance and exercise endurance and to maintain healthy lung function. Betacryptoxanthin extract is prepared by a cost and time effective process of saponification and column chromatography, in which saponification cycle time, solvent amount, column separation time and silica amount are reduced, thus making it industrially viable.
Omniactive Health Technologies Limited
A rotary valve comprising a stator and a rotor wherein the stator comprises at least three inlet primary connection ports, at least three outlet primary connection ports, at least three inlet secondary connection ports and at least three outlet secondary connection ports, and wherein rotor interconnection paths are arranged to in the different rotor positions interconnect the inlet primary connection ports with the inlet secondary connection ports and the outlet primary connection ports with the outlet secondary connection ports such that all of at least three inlet secondary connection ports can be connected one at the time to each of at least three inlet primary connection port and all of at least three outlet secondary connection ports can be connected one at the time to each of at least three outlet primary connection port by rotating the rotor into the different rotor positions. A chromatography system comprising at least three chromatography columns, a rotary valve connected to the inlets of at least three columns in the system and to at least three inflows and connected to the outlets of at least three columns in the system and to at least three outflows, and a feed recirculation flow path..
Ge Healthcare Bio-sciences Ab
Manifold connection assembly
A manifold assembly has a manifold and can be located between a block and a plate, and serve as a stator for a valve or other component. The manifold, block or plate can have a plurality of ports either integral thereto or removably connected thereto, with the ports adapted to receive and sealingly engage with a tube having an inner tube layer, an outer tube layer, a sleeve, a tip portion, and a nut.
Idex Health & Science Llc
Method for purifying recombinant fsh
The present invention relates to a method for purifying a recombinant follicle stimulating hormone (fsh) or recombinant fsh variant. The method comprises the steps of subjecting a liquid containing a recombinant fsh or recombinant fsh variant to an anion exchange chromatography, to a hydrophobic interaction chromatography, and to a dye affinity chromatography, wherein these chromatographies may be performed in any order, and wherein the method neither comprises a weak anion exchange chromatography nor a reverse phase chromatography.
Cell penetrating protein adaptor molecules and their application in research and medicine
Cell penetrating peptides (cpps) are established as a strategy to move cargoes into the interior of eukaryotic cells by engaging import machinery on the cell surface. In most cases the cpp is covalently linked to the cargo; it is common to express cargo proteins with a cpp extension.
Kennesaw State University Research And Service Foundation, Inc.
A process for preparing succinic acid and succinate ester
This invention relates to a process for preparing succinic acid and succinate ester from a succinic acid salt in fermentation broth. In the first stage of this invention, renewable carbon resources are utilized to produce succinic acid through biological fermentation.
Composite polyamide membrane having preferred azo content
A thin film composite polyamide membrane comprising a porous support and a thin film polyamide layer characterized by possessing an azo (—n═n—) content of from 0.75% to 0.95%, as measured by pyrolysis gas chromatography.. .
Dow Global Technologies Llc
Method for analysis of trace levels of chemical additives in oil recovery production fluids
Disclosed is a method for analyzing and/or monitoring trace levels of chemical additives in production fluids for oil recovery processes, specifically from heavy oil and/or oil sands. The method comprises and extraction step coupled with a multi-dimensional gas chromatography analysis..
Dow Globaltechnologies Llc
Polyimide precursor, polyimide, polyimide film, varnish, and substrate
A polyimide precursor obtained from a tetracarboxylic acid component including norbornane-2-spiro-α-cyclopentanone-α′-spiro-2″-norbornane-5,5″,6,6″-tetracarboxylic dianhydride, or a derivative thereof, and a diamine component including a diamine, or a derivative thereof, the norbornane-2-spiro-α-cyclopentanone-α′-spiro-2″-norbornane-5,5″,6,6″-tetracarboxylic dianhydride characterized in that the ratio of the peak area of a certain peak is 60% or more in a gas chromatogram obtained by conducting gas chromatography analysis.. .
Ube Industries, Ltd.
Immunochromatography strip sensor capable of measuring biomaterial concentration over broad concentration range
The present invention relates to an immunochromatography strip sensor capable of measuring a biomaterial concentration over a broad concentration range, and a method for measuring a biomaterial concentration over a broad concentration range using the sensor. A detection method using the sensor according to the present invention can accurately measure an antigen concentration over a broad concentration range, and achieve a low cost, rapidity and convenience, and thus the method is suitable for a point of care test (poct) requiring rapidity and high sensitivity..
Multi-well cuvette provided with integrated reaction and detection means
The present application discloses a multi-well cuvette for integrating the reaction between a sample and a reagent with the detection of a specific analyte included therein, and the multi-well cuvette comprises: a reaction chamber part having a sample and a reagent placed therein; and a detection part for detecting the reaction between the sample and the reagent, wherein the reaction chamber part comprises: an extraction member standby chamber in which a member for dividing or distributing the sample is on standby; a sample filling chamber; and a reagent filling chamber which is filled with the reagent, and in which the reaction with the sample is carried out, wherein the detection part comprises: a chromatography analysis means for a reactive product between the sample and the reagent; and a detection chamber for accommodating the same.. .
Boditech Med Inc.
Process of cloning and further purification to make a recombinant intravenous immunoglobulin
The present subject matter is directed to a process of cloning and purifying recombinant intravenous immunoglobulin (ivig), comprising cloning a target gene of human immunoglobulin; in vitro screening of a yeast cell expressing the target gene of human immunoglobulin to create a yeast cell line; fermenting the yeast cell line and collecting a resulting culture medium; filtering the culture medium; undergoing weak anion exchange chromatography to collect a flow-through solution; ultra-filtrating the flow-through solution to reach a desired protein concentration; aseptic filtrating the flow-through solution; nano filtrating the flow-through solution for virus removal; and filling and incubating the flow-through solution at low ph for virus inactivation to obtain a purified recombinant ivig. The present subject matter is directed to purified recombinant ivig having five newly-found proteins, namely kh 33, kh 34, kh 35, kh 36, and kh 37 for both liquid and lyophilized forms..
Method of manufacturing intravenous immunoglobulin from fraction iii
The present subject matter is directed to a method of manufacturing purified ivig from fraction iii of plasma, comprising re-constituting a fraction iii paste in a buffer; adjusting the ph and temperature; adding ethanol and then gradually lowering the temperature; centrifuging and filtering the supernatant; ultra-filtrating to remove alcohol; undergoing weak anion exchange chromatography; ultra-filtrating to reach a desired protein concentration; aseptic filtrating; nano filtrating for virus removal; and incubating at low ph for virus inactivation to obtain a resulting fraction iii suspension comprising purified ivig. The present subject matter is directed to ivig having 14 newly-found proteins, namely kh 26, kh 27, kh 28, kh 29, kh 30, kh 31, kh 32, kh 33, kh 39, kh 40, kh 41, kh 42, kh 43, and kh 44 for both liquid and lyophilized form..
Method for protein purification under denaturing conditions
The invention relates to a method for the preparation of an application buffer for the purification of proteins by means of immobilized metal ion affinity chromatography (imac) under denaturing conditions, which is characterized in that a defined amount of a buffer concentrate having a defined ph value is mixed with a defined amount of a urea concentrate, whereby an application buffer having a defined ph value is provided. According to the invention, a corresponding kit is provided in addition.
The present invention provides a process for preparing a functionalised polymeric chromatography medium, which process comprises (i) providing two or more non-woven sheets stacked one on top of the other, each said sheet comprising one or more polymer nanofibres, (ii) simultaneously heating and pressing the stack of sheets to fuse points of contact between the nanofibres of adjacent sheets, and (iii) contacting the pressed and heated product with a reagent which functionalises the product of step (ii) as a chromatography medium.. .