|| List of recent Chromatography-related patents
| Preparation method of 1-palmitoyl-3-acetylglycerol, and preparation method of 1-palmitoyl-2-linoleoyl-3-acetylglycerol using same|
Disclosed are a method for preparing 1-palmitoyl-3-acetylglycerol in high purity and high yield without a purification process using a column chromatography, and a method for preparing 1-palmitoyl-2-linoleoyl-3-acetylglycerol in high purity and high yield using the same as a key intermediate. The method for preparing 1-palmitoyl-3-acetyl glycerol comprises the steps of: forming a reaction mixture including 1-palmitoyl-3-acetyl glycerol of the formula 1 in the specification by reacting 1-palmitoylglycerol of the formula 2 in the specification and an acetylating agent; and separating the optically active 1-palmitoyl-3-acetylglycerol by crystallizing the reaction mixture in a saturated hydrocarbon solvent having 5 to 7 carbon atoms..
| Method for the enrichment of rebaudioside b and/or rebaudioside d in stevia-derived glycoside compositions using adsorb-desorb chromatography with a macroporous neutral adsorbent resin|
The invention relates to the use of adsorb/desorb chromatography to prepare enriched compositions comprising rebaudioside b and/or rebaudioside d. Compositions with enriched rebaudioside-b and/or rebaudioside-d components may be prepared from stevia-derived glycoside compositions using an adsorb-desorb chromatography process where the stationary phase of the chromatography bed comprises a macroporous neutral adsorbent resin..
| Method for the purification of antibodies|
A method for the purification of immunoglobulins by ion exchange chromatography is described. The chromatographic method uses a weak ion exchange resin and a single step elution process for the purification of an immunoglobulin.
| Purification of immunoglobulins using affinity chromatography and peptide ligands|
An immunoglobulin binding peptide having the general formula, from amino terminus to carboxy terminus, of z—r1—r2—r3—r4—r5—r6—x, is described, wherein: r1 is h or y; r2 is a hydrophobic, preferentially aromatic, amino acid (for example w, f, y, v); r3 is a positively charged or aromatic amino acid (for example r, h, f, w); r4 is a hydrophobic or positively charged amino acid (for example g, y, r, k, l); r5 is a positively charged or aromatic amino acid (for example w, f, r, h, y); r6 a random amino acid but preferably hydrophobic or negatively charged (for example v, w, l, d, h); x is present or absent and when present is a linking group; and z is present or absent and when present is a capping group bonded to the n terminus of r1; and wherein the amino acids of said peptide are in d form, l form, or a combination thereof. Methods of using such peptides for the purification of immunoglobulins are also described.
| Kit for immuno-chromatography, reagent for immuno-chromatography, and method of detecting using them|
A kit for immunochromatography, having: a planar test strip having a test area and a reference area, the test area having a test purpose capturing substance, the reference area having a reference purpose capturing substance; fluorescent particulates provided with a binding property to a target substance, the target substance provided with a binding property to the test purpose capturing substance; and light absorbing particulates provided with a binding property to the reference purpose capturing substance.. .
| Chromatography method, chromatography kit, and method of producing an insoluble carrier for chromatography|
A chromatography method including a step of developing a complex of a specimen and a labeled substance modified by a first bondable substance which can be bonded to the specimen on an insoluble carrier, a step of capturing the complex of the specimen and the labeled substance at a reactive site on the insoluble carrier, a step of washing the reactive site on the insoluble carrier using a washing liquid, a step of detecting the specimen which is captured at the reactive site after a signal of the labeled substance is amplified by an amplification liquid, in which the reactive site on the insoluble carrier includes (i) a second bondable substance which can be bonded to the specimen or a substance having affinity to the first bondable substance which can be bonded to the specimen, and (ii) polyethylene glycol having the molecular weight from 1,500 to 10,000.. .
| Glycoproteins having lipid mobilising properties and therapeutic applications thereof|
A biologically active lipid mobilising agent for use in therapy is disclosed which has the properties and characteristics of a zn-α2-glycoprotein, or of a fragment thereof having an apparent molecular mass mr greater than 6.0 kda as determined by gel exclusion chromatography. Methods of isolation and purification from biological material are also disclosed together with uses of the material for making up pharmaceutical compositions, especially pharmaceutical compositions useful for treating mammals to achieve weight reduction or for controlling obesity.
| Mixed-mode chromatography membranes|
Described are composite materials and methods of using them for mixed-mode chromatography. In certain embodiments, the composite material comprises a support member, comprising a plurality of pores extending through the support member; and a multi-functional cross-linked gel.
| Method for measuring stable hemoglobin a1c using liquid chromatography, and method for simultaneous measurement of stable hemoglobin a1c and abnormal hemoglobin using liquid chromatography|
The present invention is a method for measuring stable hemoglobin a1c using liquid chromatography, installing on a flow path of a liquid chromatograph a filter whose surface is treated with a solution containing 1 to 50% by weight of a silicone oil or a solution containing 1 to 50% by weight of a silicone resin, and setting a pressure value generated in a measurement system of the liquid chromatograph to 9.8×103 pa or more and 19.6×105 pa or less.. .
|Turbulent flow mixing device for use in a chromatography system|
A mixing device for use in a chromatography system, the device includes an exterior housing having a first end and a second end and a hydraulic flow connector at the first end of the exterior housing. A cartridge including a chamber is enclosed within the exterior housing.
|Quality control reagents and methods|
The present invention provides reagents for instrumentation quality control and methods of use thereof. In particular, sets of peptides or other molecules are provided for evaluating the performance of instruments with mass spectrometry (ms) and/or liquid chromatography (lc) functionalities..
|Method for producing peptide fractions and use thereof|
The invention relates to a method for producing enriched peptide fractions from protein-containing raw materials, in which protein hydrolysates are separated using chromatography, according to the physiochemical properties thereof, by means of stationary phases with an aqueous solution as an elution agent.. .
|Separation of acetylated proteins from unacetylated proteins|
The invention relates to a process for separating acetylated proteins from unacetylated proteins. In particular, the invention relates to a process of using multimodal chromatography to separate acetylated proteins from unacetylated proteins.
|Integrated modular unit including an analyte concentrator-microreactor device connected to a cartridge-cassette|
The present invention relates to an immunoaffinity device for capturing one or more analytes present at high or low concentrations in simple or complex matrices. The device is designed as an integrated modular unit and connected to capillary electrophoresis or liquid chromatography for the isolation, enrichment, separation and identification of polymeric macromolecules, primarily protein biomarkers.
|Purification of biological products by constrained cohydration chromatography|
Materials and methods for use of constrained cohydration agents in the purification of biological materials such as antibodies, viruses, cells, and cellular organelles in connection with convective chromatography, fluidized bed or co-precipitation applications.. .
|Preparation of pre-coated rp-rotors and universal chromatorotors, chromatographic separation devices and methods for centrifugal preparative chromatography|
Reversed phase solid silica gel sorbent layers which can adhere with appropriate binders to a solid surface were developed. Pre-coated rp-rotors of different thickness were used these pre-coated rp-rotors can be used for centrifugal preparative thin-layer chromatography (cptlc) using any appropriate device for centrifugal chromatography, like chromatotron® or cyclograph.
|Chromatography columns, systems and methods|
The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column..
|Packing of chromatography columns|
The invention provides a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column..
|Method and apparatus for desolvating flowing liquid|
Methods and apparatus for desolvating flowing liquid streams while retaining temporal resolution of dissolved substrates are disclosed. A novel small-scale self-regulating spray dryer preserves temporal resolution while desolvating a liquid chromatography eluent stream and depositing the solute onto an optical surface for infrared spectrographic analysis.
|Apparatus and methods for fluid processing and flow control|
Fluid processing apparatus has a prefabricated branched network of flexible tubing, for conducting process fluid between process elements of the apparatus, and control valves. A tubing support has opposable front and rear plates which define a pattern of support channels between them in which the flexible tubing network lies, so that the support channels limit or prevent expansion of the flexible tubes.
|Purification of not-glycosylated polypeptides|
The current invention reports a method for the purification of a not-glycosylated, heterologous polypeptide, which has been recombinantly produced in a prokaryotic cell, wherein the method comprises three chromatography steps of which the first chromatography step selected from i) hydrophobic charge induction chromatography, or ii) hydrophobic interaction chromatography, or iii) affinity chromatography, or iv) ion exchange chromatography, the second chromatography step is selected from i) anion exchange chromatography, or ii) cation exchange chromatography, or iii) hydroxylapatite chromatography, or iv) hydrophobic interaction chromatography, and the a third chromatography step is selected from i) hydrophobic charge induction chromatography, or ii) anion exchange chromatography, or iii) cation exchange chromatography, or iv) hydrophobic interaction chromatography, whereby the first chromatography step is an affinity chromatography in case of polypeptides capable of interacting with metal ligands, the second chromatography step is not a hydroxylapatite chromatography step in case of polypeptides with an isoelectric point below 6.0, and the third chromatography step can be performed in flow-through mode with polypeptides having a low or high isoelectric point.. .
|Porous material and devices for performing separations, filtrations, and catalysis and ek pumps, and mthods of making and using the same|
Embodiments of the present invention are directed to a porous monolith polymeric composition having utility in catalysis, chromatography, filtration, and electro-kinetic pumps, devices incorporating such composition and methods or making and using such monoliths. The monoliths are characterized by a substantially homogeneous skeletal core with little shrinkage, few voids and few channels..
|Sequential low/high-resolution library search|
Provided herein are systems and method for using unit mass library searches for sample identification in accurate mass spectrometry. In general, the systems and methods described herein: (a) obtain an accurate mass spectrum of a sample; (b) calculate a unit mass spectrum based on the accurate mass spectrum; and (c) conduct a unit mass library search, based on the calculated unit masses, to obtain at least one candidate species to thereby identify the sample.
|Electrospray emitter assemblies for microfluidic chromatography apparatus|
An apparatus for chemical separations includes a microfluidic substrate having an outlet aperture for outputting an eluent of a sample. An emitter assembly includes having a deformable end portion, an inlet near the deformable end portion to receive the sample eluent from the microfluidic substrate, and an electrically conductive outlet portion to emit a spray of the sample eluent.
|Methods and devices for open-bed atmospheric collection for supercritical fluid chromatography|
A supercritical fluid chromatography system comprises a first pump for pumping a first flow stream comprising a compressible fluid and a second pump for pumping a second flow stream comprising a modifier fluid. The second pump is in parallel with the first pump.
|Gas chromatograph having an absorption spectrometer and method for analyzing a gas mixture via gas chromatography|
A method in which a sample of a gas mixture to be analyzed via gas chromatography is conducted through a chromatographic separating device via a carrier gas, separated components of the gas mixture are subsequently quantitatively determined in an absorption spectrometer having a wavelength-adaptable light source, and in order to increase the speed of analysis and to be able to also determine components that cannot be measured via absorption spectroscopy, the wavelength of the light source can be adapted to an absorption line of the carrier gas, where the individual components of the gas mixture are determined indirectly via a concentration reduction of the carrier gas.. .
|Process for separation of oligonucleotide of interest from a mixture|
The present invention is directed to a method for separation of an oligonucleotide from a mixture using a biphasic mobile phase/stationary phase liquid-liquid chromatography system. A first mobile phase contains the oligonucleotide and the stationary phase contains an exchanger substance that removably binds to the target oligonucleotide.
|Methods and systems for determining the amount of thiopurine metabolites in a sample|
Disclosed are methods and systems for the analysis of thiopurine drug metabolites in a sample using liquid chromatography/mass spectrometry.. .
|Fluorescence assay for ghrelin o-acyltransferase activity|
An assay to detect ghrelin o-acyltransferase activity using an acrylodan-labeled peptide mimic of ghrelin that provides for high-throughput screening for ghrelin o-acyltransferase inhibitors and detection via high performance liquid chromatography. Alternatively, the assay for ghrelin acylation may be based on a synthetic peptide substrate that mimics the n-terminal sequence of ghrelin and has an environmentally-sensitive fluorophore attached to its c-terminal amino acid through chemoselective ligation..
|Analysis of dried blood spot samples in a microfluidic system with dilution of extracted samples|
An apparatus for use in a chromatography system includes a microfluidic substrate having a fluidic channel configured as an analytical chromatographic column and a fluidic port on one side of the microfluidic substrate. The fluidic port opens at a head end of the analytical chromatographic column.
|Device, system and method for in-vivo immunoassay|
In vivo devices, systems and methods for in vivo immunoassay include inserting into a patient's body lumen an in vivo diagnostic device comprising a housing. The housing of the device comprises a chamber, and a chromatography strip for immunoassay of a body lumen substance.
|Immunochromatography detection sensor comprising optical waveguide and a detection method using the same|
The present invention relates to an immunochromatographic detection sensor comprising optical waveguides and a detection method using the same, and more particularly, to an immunochromatographic detection sensor comprising optical waveguides, in which the optical waveguides are provided under the membrane, probe beams transmitted through the optical waveguide maximize the interaction frequency between evanescent wave generated on the surface of the optical waveguide and the colored conjugate in the band formed on the membrane, resulting in the absorbance signal from the colored conjugate being greatly amplified to improve the sample detection sensitivity, and to a detection method using the same.. .
|Pure albumin and its method of preparation and detection|
The subject of the present invention is a pure monomeric bovine serum albumin, a method of producing it characterised by the use column chromatography in resin and a method of identifying it using dynamic light scattering.. .
|Methods for purification of arylsulfatase a|
Methods of producing arylsulfatase a are described herein. The methods can include one or more steps of chromatography.
|Method of extracting lutein/xanthophylls from natural materials and highly purified lutein/xanthophylls obtained from the method thereof|
The present invention provides the new method for extracting lutein from natural materials wherein the said method comprises of modification of natural lutein ester in the natural materials to free lutein and/or low molecular weight lutein ester, extraction of the said natural materials with supercritical fluid at the optimal conditions. The said method yields high amount of crude lutein with high purity due to the mild condition used for extraction.
The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (pufa) product from a feed mixture, which process comprises the steps of: (i) purifying the feed mixture in a first separation step in a simulated or actual moving bed chromatography apparatus having a plurality of linked chromatography columns containing, as eluent, an aqueous organic solvent, to obtain an intermediate product; and (ii) purifying the intermediate product obtained in (i) in a second separation step using a simulated or actual moving bed chromatography apparatus having a plurality of linked chromatography columns containing, as eluent, an aqueous organic solvent, to obtain the pufa product; wherein (a) the first and second separation steps are carried out sequentially on the same chromatography apparatus, the intermediate product being recovered between the first and second separation steps and the process conditions in the chromatography apparatus being adjusted between the first and second separation steps such that the pufa product is separated from different components of the feed mixture in each separation step; or (b) the first and second separation steps are carried out on separate first and second chromatography apparatuses respectively, the intermediate product obtained from the first separation step being introduced into the second chromatography apparatus, and the pufa product being separated from different components of the feed mixture in each separation step.. .
|Isotopic recoding for targeted tandem mass spectrometry|
Aspects of the present disclosure include methods for detecting a low abundance protein and methods for identifying a site of n-glycosylation on a protein. In practicing methods according to certain embodiments, a eukaryotic cell is contacted with an isotopic labeling composition and isotopically labeled n-glycosylated peptides obtained from the eukaryotic cell are assessed by liquid chromatography-tandem mass spectrometry.
|Electrode for nonaqueous electrolyte secondary battery, nonaqueous electrolyte secondary battery, and battery pack|
An electrode for a nonaqueous electrolyte secondary battery of an embodiment has an active material layer containing an active material and a binder containing fluorine, and a current collector bound to the active material layer. When a thermal decomposition start temperature of the binder is t1° c.
|Pump and injector for liquid chromatography|
A combined pump-injector valve utilizing a single piece as the barrel of the pump and as the stator of the valve, thus eliminating any need for connections between a pump and a valve, and therefore the potential for high-pressure leaks or pressure reductions. The combined pump-injector valve permits injection of nanoliter-sized samples into a chromatographic column, which is sealed during loading of the sample and filling of the pump, such that complete analyses can be completed with microliters of mobile phase with nanoliters of a sample..
|Pipe containing a metal casing with a plastics material inlay for use in low and high pressure applications, in particular as an hplc column|
A chromatography column comprises a pipe that contains a tubular metal casing with an inlay and sealing ring. The inlay is configured as a plastics material tube and is pushed or drawn into the metal casing and a sealing ring of plastics material is connected to the inlay at the end.
The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (pufa) product, from a feed mixture, which process comprises introducing the feed mixture to a simulated or actual moving bed chromatography apparatus having a plurality of linked chromatography columns containing, as eluent, an aqueous organic solvent, wherein the apparatus has a plurality of zones comprising at least a first zone and second zone, each zone having an extract stream and a raffinate stream from which liquid can be collected from said plurality of linked chromatography columns, and wherein (a) a raffinate stream containing the pufa product together with more polar components is collected from a column in the first zone and introduced to a nonadjacent column in the second zone, and/or (b) an extract stream containing the pufa product together with less polar components is collected from a column in the second zone and introduced to a nonadjacent column in the first zone, said pufa product being separated from different components of the feed mixture in each zone, and wherein the aqueous organic solvent is other than aqueous alcohol.. .
|Methods of purification of native or mutant forms of diphtheria toxin|
The present invention relates to the use of hydroxyapatite chromatography and multimodal chromatography, for punfication of diphtheria toxin, or a mutant form thereof, from a mixture, for example, a host cell fermentation mixture containing impurities such as host cell proteins and dna. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of diphtheria toxin suitable for in vitro and in vivo applications..
|Chromatography filter paper-based elisa|
A chromatography filter paper-based elisa includes following steps: providing a chromatography filter paper plate provided with a plurality of independent hydrophilic regions defined by at least one hydrophobic region; immobilizing an antigen of a sample onto one of the hydrophilic regions of the chromatography filter paper plate; and detecting the antigen with an elisa process, wherein an antibody used in the elisa process is a monoclonal antibody. The present invention has advantages in lower amount of loaded sample, higher sensitivity and rapid analysis in comparison to conventional elisa..
|Non-invasive detection of fetal genetic traits|
Blood plasma of pregnant women contains fetal and (generally>90%) maternal circulatory extracellular dna. Most of said fetal dna contains 500 base pairs, said maternal dna having a greater size.
|Device for controlling a piston pump unit for liquid chromatography|
A control device of a piston pump unit comprising at least two piston-cylinder units that operate in a phase-shifted manner for the purpose of liquid chromatography and to a piston pump unit is described. The control device corrects fluctuations of the system pressure while switching from one piston cylinder unit to the respective other piston cylinder unit.
|Vacuum ultraviolet absorption spectroscopy system and method|
An efficient absorption spectroscopy system is provided. The spectroscopy system may be configured to measure solid, liquid or gaseous samples.
|Rotary shear valve with a two-pin drive shaft for liquid chromatography applications|
A rotary shear valve assembly for liquid chromatography applications comprises a rotor assembly having a rotor and a drive shaft with a head portion. The rotor has a substantially planar surface with one or more rotor grooves and a pair of holes.
|Chromatography system with led-based light source|
A detector system having a single emitter body. The emitter body has a plurality of light emitting diodes (leds) for emitting a plurality of wavelengths.
|Collection system for purification flowstreams|
A collection system for collecting samples from a flowstream (5) exiting a supercritical fluid chromatography system is provided. The collection system comprises: (i) a first back pressure regulator (10) on the flowstream as it exits the chromatography system, (ii) a gas-liquid separator (30) having a tapered and angled dripper (65), which introduces the flow into the separator at an angle tangential to the separator wall; and (iii) one or more fraction collectors (15), wherein the fraction collector is at a reduced pressure collection point, between 100 bar and atmospheric pressure.
|Efficient chiller for a supercritical fluid chromatography pump|
Methods and systems for pumping compressible fluids in high pressure applications such as high-pressure liquid chromatography (hplc) or supercritical fluid chromatography (sfc) applications are disclosed. An improved cooling device for a pump head for use in a supercritical fluid chromatography (sfc) system is described.
|Purification of fusion proteins|
The invention relates generally to methods for purifying a fc-fusion protein produced in a eukaryotic expression system. More specifically, the invention provides a robust and scalable downstream purification process suitable for use in manufacturing tnfr:fc for human administration which comprises an optimized protein a affinity chromatography step and two ion exchange chromatography steps both of which are operated in the bind-and-elute mode..
|Single unit chromatography antibody purification|
The present invention relates to a method for the purification of antibodies from a protein mixture produced in a bioreactor, at least comprising the steps of intermediate purification and polishing, wherein the intermediate and polishing step comprises in-line anion exchange chromatography (aex) treatment and mixed mode chromatography (mimo) treatment in flow through mode. The present invention further relates to a single operational unit comprising both an anion exchange chromatography part and a mixed mode chromatography part, which are serially connected, wherein the unit comprises an inlet at the upstream end of the anion exchange chromatography part and an outlet at the downstream end of the mixed mode chromatography part and wherein the unit also comprises an inlet between the anion exchange chromatography part and the mixed mode chromatography part..
|Low ph protein purification process|
The invention relates to a process for recovering and purifying bile salt-stimulated lipase (bssl) in a solution which contains impurities, said process comprising the steps: (i) applying bssl to a hydrophobic interaction chromatography (hic) resin; (ii) removing impurities by washing said hic resin with a wash composition having a ph in the range from 4 to 5; and (iii) recovering bssl from said hic resin.. .
|Uncoated housing and method for manufacturing same|
The present invention is an uncoated housing in which a molded product formed from a thermoplastic resin composition (d) containing 20-60% by mass of an acetone insoluble resin component (a), which contains a rubber component (a) with an average particle size of 0.05-0.3 μm and has a coefficient of linear expansion of 11×10−5-20.5×10−5, 40-80% by mass of an acetone soluble resin component (b) (here, (a)+(b)=100% by mass), and a colorant (c), with (1) the acetone soluble resin component (b) containing 6.0-45% by mass of an unsaturated nitrile monomer derived component and (2) 2-20% by mass of a component with a molecular weight of 70,000 or less by gel permeation chromatography (gpc) measurements being contained in the acetone soluble resin component (b). On the basis of jis k7105, the l* value for this uncoated housing is 13 or less, the luster 60-120%, and the haze value 30-90%..
|Graft copolymer for cation-exchange chromatography|
The invention relates to chromatographic separating materials having improved binding capacity for biological constituents in cell culture supernatants, or animal or human body fluids, in particular for monoclonal antibodies. The present invention likewise relates to the preparation of separating materials of this type, and to the use thereof, in particular for the removal of charged biopolymers from corresponding liquids..
|Sonication for improved particle size distribution of core-shell particles|
In one or more embodiments, a porous composite particulate material includes a plurality of composite particles including an acid-base-resistant core particle at least partially surrounded by one or more layers of acid-base-resistant shell particles. The shell particles are adhered to the core particle by a polymeric material.
|Chromatography column and method for isolating nucleic acid|
The present invention provides a chromatography column and a method for isolating nucleic acid molecules. In one embodiment, the present invention provides a double-layer column of a first anion exchange membrane and a second serially coupled silica membrane.
|Protein purification using hcic and ion exchange chromatography|
The present invention provides methods for purifying proteins. In particular, the methods employ a two-step non-affinity chromatography process without the use of an in-process tangential flow filtration step..
|Viral purification methods|
The present invention is directed to an improved method of purifying virus, particularly reovirus. Infectious virus can be extracted from a cell culture with a detergent to produce high titers of virus, and the virus can then be purified by simple steps such as filtration and column chromatography.
|Method for separating peptides and proteins|
Embodiments of the present invention are directed to articles of manufacture, devices, methods and apparatus for performing liquid chromatography featuring a chromatographic sorbent having one or more pentafluorophenyl groups, wherein said one or more pentafluorophenyl groups are a bonded phase on a sorbent selected from the group comprising silica, organic polymers or hybrid organic silane material and said pentafluorophenyl groups are in a mono-, bi-, and tridentate forms.. .
|Separation of glycans by mixed-mode liquid chromatography|
An exemplary multimodal chromatographic medium of the invention includes one or more strong anion exchange, weak anion exchange, strong cation exchange and/or weak cation exchange binding sites in combination with one or more reverse phase and/or hydrophilic interaction chromatography binding site. In an exemplary embodiment, the sites interact with one or more glycans in a mixture of glycans in a manner that allows separation of glycans in the mixture and analysis of the glycan mixture.
|Capture of pathogenic and non-pathogenic biopolymers and bioparticles|
B) providing a support matrix modified to have, at predetermined conditions, such a net negative surface charge and net surface hydrophobicity in relation to the net negative surface charge and the net surface hydrophobicity of the selected biopolymer or bioparticle that the modified support matrix is capable of selective interaction and capture of the biopolymer or bioparticle through a resulting net hydrophobic interaction being the actual hydrophobic interaction minus the actual electrostatic repulsion. The use of such a capturing agent as a therapeutically active substance, as well as in chromatography and diagnosis are also disclosed..
|Fluid sample holders with piston valve|
Disclosed is a fluid sample holder 20 for delivering or receiving a fluid sample to or from fluid processing equipment such as a chromatography column 5 (fig. 1).
|Fluid sample holders with piston valve|
Disclosed is a fluid sample holder 20 for delivering or receiving a fluid sample to or from fluid processing equipment such as a chromatography column 5 (fig. 1).
|Microfluidic device with dried blood spots (dbs) card interface|
An apparatus for use in a chromatography system includes a first microfluidic substrate having a first fluidic channel. One end of the first fluidic channel terminates at a first fluidic port on a first side of the first microfluidic substrate and an opposite end of the first fluidic channel terminates at a second fluidic port on a second side of the first microfluidic substrate.
|Thin layer chromatography plates and related methods of manufacture including priming prior to infiltration with stationary phase and/or precursor thereof|
In an embodiment, a method for manufacturing a thin layer chromatography (“tlc”) plate is disclosed. The method includes forming a layer of elongated nanostructures (e.g., carbon nanotubes), priming the elongated nanostructures with one or more adhesion priming layers, and at least partially coating the elongated nanostructures with a coating.
|Chromatography column assembly|
Described is a chromatography column assembly that includes a permanently deformable outer tube, an intermediate tube, an inner tube and a sorbent bed disposed within the inner tube. The sorbent bed may be in the form of packed chromatographic particles or a porous monolithic structure.
|Control system and method for flash separation|
A chromatography system may include: a first solvent supply operatively connected to a chromatography column; a second solvent supply operatively connected to the chromatography column; a controller operatively connected to the first and second solvent supplies for delivering a flow of a solvent system to an inlet for the chromatography column in accord with a predetermined solvent gradient, wherein the flow of the solvent system through the chromatography column will separate components from a sample composition; and a collection apparatus operatively connected to an outlet from the chromatography column for collecting designated portions of the solvent system flow exiting the column. The controller may determine a target sample mass as a function of chromatography column parameters..
There is provided a capillary column comprising a flat cross section and a desired theoretical plate number and having both high resolution and high sample load capacity. The capillary column 1 comprising a stationary phase on an inactivated inner surface, which is used in gas chromatography, comprises a narrow part 3 formed in a central part of a cross section of internal space and a bulge part 4 formed on each of both sides of the narrow part 3..
|Method for purifying pegylated erythropoietin|
Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly (ethylene glycol) residue from reaction by-products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange sp sephacryl™ s 500 hr chromatography material conditioned to a conductivity of 21 ms/cm and a linear gradient elution a fusion protein of erythropoietin and a single poly (ethylene glycol) residue can be obtained in a single step with high purity and yield..
|Preparing chloride-free polyethyleneimines|
Polyalkyleneimines obtained by such methods and formulations thereof likewise form part of the subject matter of the invention, especially those having a low proportion of chloride-containing compounds. Such polyalkyleneimines have uses in the field of medical technology, printing media, wastewater treatment, surface treatment, cosmetics, laundry detergents, biotechnology, packaging, electronics, paper, building construction chemistry, textiles, chromatography, ion exchangers, oil industry, ceramics, glass, membrane technology, catalysts, electroplating, biocides or wood protection.