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Chromatography patents

This page is updated frequently with new Chromatography-related patent applications. Subscribe to the Chromatography RSS feed to automatically get the update: related Chromatography RSS feeds. RSS updates for this page: Chromatography RSS RSS

Date/App# patent app List of recent Chromatography-related patents
 Highly aromatic base oil and  producing highly aromatic base oil patent thumbnailnew patent Highly aromatic base oil and producing highly aromatic base oil
A method for producing a highly aromatic base oil of the present invention includes a step of hydrorefining a clarified oil to obtain a highly aromatic base oil having an aromatic content of 50% by mass or more determined by a column chromatography analysis method. The step of hydrorefining a clarified oil is preferably performed under conditions of a hydrogen pressure of 5.0 to 20.0 mpa, a temperature of 280 to 400° c., a hydrogen oil ratio of 300 to 750 nl/l, and a space velocity of 0.3 to 2.0 h−1.
Jx Nippon Oil & Energy Corporation
 Process for the purification of recombinant albumin patent thumbnailnew patent Process for the purification of recombinant albumin
A process is provided for the preparation of a highly pure albumin solution the process comprising subjecting albumin (preferably expressed and secreted by transformed yeast) to a series of chromatographic steps. Preferably, the process comprises the steps of positive mode cation exchange chromatography, positive mode anion exchange chromatography, positive mode affinity chromatography, negative mode affinity chromatography (preferably using immobilised aminophenylboronic acid), negative mode cation exchange chromatography, and negative or positive mode anion exchange chromatography.
Novozymes Biopharma Dk A/s
 Antibody purification by cation exchange chromatography patent thumbnailnew patent Antibody purification by cation exchange chromatography
A method for purifying an antibody by cation exchange chromatography is described in which a high ph wash step is used to remove of contaminants prior to eluting the desired antibody using an elution buffer with increased conductivity.. .
Genentech, Inc.
 Uv/vis hplc photometer patent thumbnailnew patent Uv/vis hplc photometer
An ultraviolet-visible spectrophotometry (uv/vis) high-performance liquid chromatography (hplc) photometer includes a nano flow cell in which a light source feeds a reference light channel and a sample light channel, a reference photodiode for evaluating the reference light channel, and a sample photodiode for evaluating the sample light channel, which extends through the nano flow cell. To create a photometer whose signal evaluation is improved, respective effective exposure times of the sample photodiode and the reference photodiode are settable separately from one another..
Sunchrom Wissenschaftliche Geräte Gmbh
 Corrosion protection in tubing used in chromatography patent thumbnailCorrosion protection in tubing used in chromatography
An apparatus for use in a liquid chromatography system includes a chromatography port and a tubing assembly having a chromatography tube coupled at one end to the chromatography port. The end of the tube has an end face covered with a corrosion-resistant material, for example, gold.
Waters Technologies Corporation
 Method and  sample injection in liquid chromatography patent thumbnailMethod and sample injection in liquid chromatography
The present invention provides a method and apparatus for substantially eliminating destructive transients of pressure or flow rate which can degrade the efficiency and useful lifetime of chromatography columns. The present invention enables a substantially constant flow of mobile phase liquid to be maintained through the chromatography system by eliminating the flow blockage interval associated with the actuation of sample injection valves.
Waters Technologies Corporation
 Force balance needle valve pressure regulator for carbon dioxide based chromatography patent thumbnailForce balance needle valve pressure regulator for carbon dioxide based chromatography
Exemplary embodiments of the present disclosure relate to systems and methods comprising a dynamic pressure regulator and a force balance needle that regulate pressure changes due to flow or composition changes in a pressurized flow system, such as, for example, a co2-based chromatography system.. .
Waters Technologies Corporation
 Introducing samples into supercritical fluid chromatography systems patent thumbnailIntroducing samples into supercritical fluid chromatography systems
A supercritical fluid chromatography system is provided with an injection valve subsystem for introducing a sample into a flow of mobile phase fluid. The injection valve subsystem includes an auxiliary valve and an inject valve.
Waterstechnologies Corporation
 Mass spectrometry quantitation of p450 protein isoforms in hepatocytes patent thumbnailMass spectrometry quantitation of p450 protein isoforms in hepatocytes
A method for screening a drug for cytochrome p450 (cyp) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome p450 isoform. The isoforms can be selected from 2b6, 3a4, 1a2, and 3a5 isoforms.
Dh Technologies Development Pte. Ltd.
 On-column enzymatic cleavage patent thumbnailOn-column enzymatic cleavage
Herein is reported a method for obtaining a polypeptide by an immobilized metal ion affinity chromatography from a pro-polypeptide that comprise at its n- or c-terminus an metal ion affinity chromatography tag and a protease cleavage site comprising the step of recovering the polypeptide from the immobilized metal ion affinity chromatography column by incubating the bound pro-polypeptide with a protease, whereby the immobilized metal ion affinity chromatography material has been washed at least once with an urea solution.. .
Hoffmann-la Roche Inc.

Method for analyzing formyl glycine residue

Disclosed is a method which enables semiquantitative or quantitative determination of the ratio between cysteine and formylglycine residues in a protein. The method includes (a) a step of labeling the protein (i) with a halogen-substituted carboxylic acid, (ii) with a halogen-substituted carboxylic acid amide, and (iii) with a halogen-substituted carboxylic acid and then with hydrazine, or with a halogen-substituted carboxylic acid and then by oximation, (b) a step of digesting each labeled protein to provide a corresponding mixture of peptide fragments, (c) a step of subjecting each mixture to reverse phase chromatography to separate the peptide fragments from each other to produce a chromatogram, (d) a step of comparing the produced chromatograms with each other to identify the peak corresponding to the peptide fragment that contained a cysteine residue and the peak corresponding to the peptide fragment that contained a formylglycine residue..
Jcr Pharmaceuticals Co., Ltd.

Aqueous silane coupling agent composition, making method, surface treating agent, and article

A composition comprising a silane coupling agent having dicarboxylic acid group and water is provided. The composition has a solid content of 0.5-50 wt % based on the entire composition and a volatile organic compound content of up to 10 wt % based on volatile components which are detectable by headspace gas chromatography.
Shin-etsu Chemical Co., Ltd.

Method for extracting and separating ginkgolides

A method for extracting and separating ginkgolides. The ginkgolides obtained through steps of extraction on a ginkgo leaf, further extraction, column chromatography, crystallization, and crystal mixing comprise 25.0% to 50.0% of bilobalide (c15h18o8), 20.0% to 45.0% of ginkgolide a (c20h24o9), 10.0% to 30.0% of ginkgolide b (c20h24o10), and 5.0% to 15.0% of ginkgolide c (c20h24o11).
Chengdu Baiyu Technology Pharmacy Co., Ltd.

High pressure fitting for a liquid chromatography system

A fitting for coupling fluidic paths, such as high pressure fluidic paths in liquid chromatography systems, includes a compression nut, a tube assembly and a compression member. The compression nut has a threaded outer surface to engage a threaded bore of a receiving port and the tube assembly has an outer surface and an end face to contact a sealing surface of the receiving port.
Waters Technologies Corporation

Chromatography column

chromatography columns having at least one assembly comprising a backing plate adhesively bound to a flow distributor, and methods of making and using the columns are disclosed.. .
Pall Corporation

Process for producing a particulate composition comprising crystalline trehalose dihydrate

A process for enabling the production of a particulate composition containing crystalline trehalose dihydrate is provided. Including allowing an α-glycosyltrehalose-forming enzyme to act on liquefied starch derived from a microorganism of the genus arthrobacter and a trehalose-releasing enzyme derived from a microorganism of the genus arthrobacter along with a starch debranching enzyme and a cyclomaltodextrin glucanotransferase; allowing glucoamylase to act on the resulting mixture to obtain a saccharide solution containing α,α-trehalose; precipitating crystalline α,α-trehalose dihydrate from the above saccharide solution; collecting the precipitated crystalline α,α-trehalose dihydrate by a centrifuge; and ageing and drying the collected crystals.
Hayashibara Co., Ltd.

Regulator for improved thermal and pressure control for chromatography

Exemplary embodiments of the present disclosure include systems, apparatuses, and methods that are directed to controlling pressure in a pressurized flow system, such as a c02-based chromatography system or other pressurized flow systems. Exemplary embodiments of the present disclosure comprise one or more apparatuses, systems or methods for implementing multiple pressure regulators to control pressure.
Waters Technologies Corporation

Liquid chromatography device, liquid chromatography analysis process, and non-transitory computer-readable medium

A liquid chromatography device includes an adsorption portion that adsorbs one or more analysis components in a specimen, an analysis device that analyzes an analysis component eluted by an eluent, a main feeding device that feeds a first eluent to the adsorption portion, the first eluent eluting an analysis component, a feeding channel in fluid communication with the main feeding device and the adsorption portion, a first retention channel that retains a second eluent, the second eluent differing from the first eluent, an auxiliary feeding device that feeds the second eluent to the first retention channel, and a first switching device that switches the feeding channel to either a first channel or a second channel, the first channel allowing the first eluent to flow from the main feeding device to the adsorption portion, and the second channel allowing the second eluent to flow from the first retention channel to the adsorption portion.. .
Arkray, Inc.

Method for endotoxin removal

The present invention relates to a method for endotoxin removal from a sample comprising the following steps: combining the sample comprising one or more target molecule(s) with a chromatography media comprising beads having an inner porous core functionalized with ligands capable of binding endotoxin and an outer porous layer without functional groups and a pore size small enough to exclude the target molecule from the inner core; and collecting the sample from the media, wherein the sample comprises an endotoxin level which is at least 75% less, preferably 90% less, than before the removal and the yield of the target molecule is at least 75%.. .
Ge Healthcare Bio-sciences Ab

Controlling hydrogen-deuterium exchange on a spectrum by spectrum basis

A mass spectrometer is disclosed comprising a liquid chromatography device for separating ions. A gas phase ion-neutral reaction device is arranged downstream to perform a gas phase ion-neutral reaction such as hydrogen-deuterium exchange.
Micromass Uk Limited

Method and system for filtering gas chromatography-mass spectrometry data

This invention relates to a system for and a method of filtering at least a part of gas chromatography-mass spectrometry data, the method comprising: providing gas chromatography-mass spectrometry data (301) for a gas mixture comprising data representing one or more gas chromatography elution peaks obtained for at least one sample, and filtering the gas chromatography-mass spectrometry data (301) to reduce the amount of data, wherein the filtering comprises taking into account predetermined data representing one or more elution peaks previously determined to be false positives (305) and/or predetermined data representing one or elution peaks previously determined to be true positives (304). In this way, unreliable elution peaks are removed in an expedient manner reducing the amount of data e.g.
Koninklijke Philips N.v.

Preparation of molecular imprinted polymers by cross-linking

Provided is an improved method for preparation of insoluble molecular imprinted polymers (mips), the method comprising: a) providing soluble or semi-soluble mips that 1) substantially all bind template agents and 2) have sizes which enable their separation in a chromatographic step utilizing packed bed chromatography, b) cross-linking the template agent binding soluble mips provided in step a so as to obtain insoluble template agent binding mips, and c) optionally isolating, concentrating or purifying the mips obtained by the cross-linking in step b. In an interesting embodiment, step a includes an affinity purification procedure, which ensures that the mips provided in step a are indeed all binders of the template..
Mipsalus Aps

Methods and kits for determining protein-bound biomarkers

(a) automated prepurification of the sample using an effective amount of an acid protease under conditions that allow for digestion of one or more binding proteins; (b) applying said protease-digested sample onto an on-line spe column to capture at least part of said biomarker, followed by sequential washing of the solid phase; (c) eluting a fraction comprising said biomarker directly onto a liquid chromatography (lc) column comprising an apolar stationary phase and subjecting it to lc-ms or lc-ms-ms measurements to determine the amount of at least one biomarker; and (d) quantitating the biomarker(s). Also provided are kits for use in such method, for instance in the determination of vitamin d derivatives, testosterone and/or or melatonin..

Black toner for developing latent electrostatic image and producing the same

A black toner for developing a latent electrostatic image, including: a toner base containing a pigment, a binder resin, a releasing agent, a compound containing fluorine and a compound containing sulfur; and an external additive, wherein the toner contains the external additive on a surface of the toner base, and wherein an amount of the fluorine in the toner base is 200 ppm by mass to 600 ppm by mass as measured by combustion-ion chromatography, and an amount of the sulfur in the toner base is 1,000 ppm by mass to 1,500 ppm by mass as measured by the combustion-ion chromatography.. .

Purification of flaviviruses

The present invention provides a method to prepare purified enveloped (e.g., flavivirus) viral particle preparations employing ion exchange chromatography and tangential flow filtration.. .
Sanofi Pasteur Biologics, Llc

Application of macroporous silica synthesized by a salt-templated aerosol chromatography

The present invention discloses a silica particle having a diameter less than or equal to 2 μη, wherein the particle is spherical and comprises interconnected pores having a diameter in the range from 50 nm to 300 nm. The silica particle is preferably produced by spray pyrolysis (=spray drying) of a silica colloid.
Indiana University Research And Technology Corporation

Analytical methods for analyzing and determining impurities in dianhydrogalactitol

An improved analytical method for analysis of dianhydrogalactitol preparations provides a method for determining the purity of dianhydrogalactitol and detecting impurities in preparations of dianhydrogalactitol, as well as identifying any such impurities. The method employs high performance liquid chromatography (hplc), in particular, hplc with refractive index (ri) detection; the hplc can be followed by tandem mass spectroscopy.
Del Mar Pharmaceuticals

Controlling interdetector band broadening

Methods and apparatus for controlling interdetector band broadening during the analysis of a sample injected into a chromatography system. A column flow is diluted with a dilution flow after the sample exits the chromatography system, and the diluted sample is analyzed by one or a combination of analysis instruments such as a light scattering detector, refractive index detector, an ultraviolet absorption detector..
Wyatt Technology Corporation

Functionalized lipid modification of solid phase surfaces for use in chromatography

A solid phase for use in separation has been modified using an aqueous phase adsorption of a headgroup-modified lipid to generate analyte specific surfaces for use as a stationary phase in separations such as high performance liquid chromatography (hplc) or solid phase extraction (spe). The aliphatic moiety of the lipid adsorbs strongly to a hydrophobic solid surface, with the hydrophilic and active headgroups orienting themselves toward the more polar mobile phase, thus allowing for interactions with the desired solutes.
Clemson University

Performing chemical reactions and/or ionization during gas chromatography-mass spectrometry runs

A gas chromatography-mass spectrometry (gc-ms) method that includes performing a first gc-ms run on a sample using a gas chromatography-mass spectrometry system. Performing the first gc-ms nm includes i) passing a first flow of a carrier gas carrying a first portion of the sample through a gas chromatograph to provide a first effluent; ii) generating first ions under protonation conditions by passing the first effluent through an atmospheric pressure ionization source; iii) passing the first ions through a mass spectrometer; and iv) recording first gc-ms data for the first ions.
Waters Technologies Corporation

Method of analyzing hemoglobins

The present invention provides a method for analyzing hemoglobins by liquid chromatography which includes pre-treating a sample with an oxidant and a binder for trivalent heme iron.. .

Chromatographic isolation of cells and other complex biological materials

The present invention relates to the chromatographic isolation of a target cell or another complex biological material, in particular by column chromatography such as affinity chromatography or gel permeation chromatography. The invention employs a receptor binding reagent that binds to a receptor molecule that is located on the surface of a target cell.
Stage Cell Therapeutics Gmbh

Toners for electrostatic-image development

An emulsion polymerized agglomerated toner characterized in that the odor index of aliphatic aldehydes measured by gas chromatography is at most 300, and an emulsion polymerized agglomerated toner which is an emulsion polymerized agglomerated toner obtainable via a polymerization step, a flocculation step and an aging step, characterized in that an emulsion polymerized latex before the flocculation step is a latex having a peroxide value of at most 30.. .

Metal components with inert vapor phase coating on internal surfaces

The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for lc analyses in which the sample interacts with metal ions or surfaces.
Agilent Technologies, Inc.

Antibodies against e7 protein of human papilloma virus (hpv)

The invention discloses a method for preparing human papilloma virus protein e7 antigen (hpv e7 antigen) comprising the following steps: —providing a purified preparation of hpv protein e7; —phosphorylating the hpv protein e7 in the preparation; —purifying the phosphorylated e7 protein with an anion exchange chromatography, wherein the phosphorylated e7 protein is separated from the non-phosphorylated e7 protein by a step wherein the non-phosphorylated e7 protein stays bound to an anion exchanger during the anion exchange chromatography whereas the phosphorylated e7 protein is obtained in the eluate of the anion exchange chromatography, thereby—obtaining a purified preparation of a phosphorylated hpv protein e7 antigen. The invention further discloses antibodies specific to this antigen, a kit and method for using such antibodies in clinical diagnostics and methods for generation of such antibodies..
Valdospan Gmbh

Flow splitting in supercritical fluid chromatography systems

A method includes passing a mobile phase fluid flow comprising liquefied co2 through a separation column; then introducing a makeup fluid flow into the mobile phase fluid flow to form a mixed fluid flow; and then splitting the mixed fluid flow.. .
Waters Technologies Corporation

System for analyzing a gas mixture including at least one chromatography column

A system for analyzing a gas mixture, including at least one chromatography column, a mechanism injecting the mixture into the column, and a mechanism detecting compound(s) forming the gas mixture, the detection mechanism including at least one detector of nanosensor type of an outlet of the column and a detector of nanosensor type in the column, capable of detecting passage of the compounds. It is then possible to determine the velocity of each of the compounds within the system..
California Institute Of Technology

Fc-receptor based affinity chromatography

Herein is reported the use of an immobilized non-covalent complex of a neonatal fc receptor (fcrn) and beta-2-microglobulin (b2m) as affinity chromatography ligand in general and, for example, for the determination of the in vivo half-live of an antibody by determining the ratio of the retention times of the antibody and a reference antibody.. .
Hoffmann-la Roche Inc.

Multimodal polyolefin resin and molded product prepared therefrom

Multimodal polyolefin resin having excellent characteristics such as moldability, mechanical strength, external appearance and a polyolefin resin molded product are disclosed. The polyolefin resin satisfies all requirements of following (1) to (5), (1) density (d): 0.934 to 0.963 g/cm3, (2) melt flow index (mie, 190° c., 2.16 kg load condition): 0.01 to 1.0 g/10 minutes, (3) ratio of weight-average molecular weight (mw) and number-average molecular weight (mn) measured by gel permeation chromatography (gpc) (mw/mn, molecular weight distribution (mwd)): 12 to 60, (4) at least two peaks appear when the molecular weight of the polyolefin resin is measured with gpc, and (5) amount of polyolefin having mw of 10,000 or less exceeds 15 weight % and amount of polyolefin having mw of 1,000,000 or more exceeds 1.5 weight %, when the molecular weight of the polyolefin resin is measured with gpc..
Daelim Industrial Co., Ltd.

Chromatography systems and methods using them

Certain embodiments described herein are directed to chromatography systems that include a microfluidic device. The microfluidic device can be fluidically coupled to a switching valve to provide for selective control of fluid flow in the chromatography system.
Perkinelmer Health Sciences, Inc.

Analytical detecting sulfated oligosaccharides

The present invention describes an analytical method for detecting and quantitating poly-sulfated oligosaccharides, including fondaparinux sodium, using hydrophilic interaction ultra-performance liquid chromatography (hilic-uplc) coupled with a charged aerosol detector (cad) or a mass spectrometer (ms). This analytical method provides in-process control in a total synthesis of highly sulfated oligosaccharides by separation, quantification and mass identification.
Scinopharm Taiwan, Ltd.

Polymer and producing same, rubber composition containing polymer, and tire having rubber composition

The polymer of the present invention is a synthesized polyisoprene or an isoprene copolymer. The polymer has a number average molecular weight (mn) of 1.5 million or more when measured by using gel permeation chromatography (gpc)..
Bridgestone Corporation

Pneumatically/hydraulically actuated fluoropolymer-hplc chromatographic system for use with harsh reagents

The invention provides a high-performance liquid chromatography system, said system is controlled in temperature by running a fluid in sleeves that surround the different parts of the system. All parts in contact with the fluid are made in fluoropolymer, carbon-filled fluoropolymer, or carbon-fiber fluoropolymer.
Orlab Chromatography, Llc

Novel oragnosilica compounds

The present invention relates to novel silica materials, precursors thereof and the preparation thereof. The compounds may be used for a variety of applications, including chromatography and catalysis..
Universiteit Gent

Sulfur amino acid-containing composition

Provision of a composition stably comprising a high concentration of sulfur amino acid derived from a plant belonging to the genus allium. A method for producing a sulfur amino acid-comprising composition comprising: heating a plant belonging to the genus allium; treating the plant belonging to the genus allium thus heated with a γ-glutamyl bond cleaving enzyme; and subjecting the resulting enzyme-treated product to ion exchange chromatography..
Nisshin Pharma Inc.

Purification of nanocrystals by gel permeation chromatography and the effect of excess ligands on shell growth and ligand exchange

Methods for purifying nanocrystals via gel permeation chromatography are provided. The method can include: loading a sample solution into a chromatography column, wherein the sample solution comprises nanocrystals coated with a layer of organic ligands; using an eluent, passing the nanocrystals coated with the layer of organic ligands through a stationary phase of the chromatography column, wherein the eluent is an organic solvent in which the nanocrystals coated with the layer of organic ligands remain miscible to form a mobile phase; and collecting the mobile phase passed through the stationary phase of the chromatography column.

Purification of herpes virus

The present disclosure provides a method to prepare purified enveloped viral particle preparations employing ion ex change chromatography and tangential flow filtration.. .
Sanofi Pasteur Biologics, Llc

Method for conducting maintenance on a chromatography column

The present invention relates to methods for conducting maintenance on chromatography columns used in industrial-scale chromatography. In particular, the invention is concerned with safer methods for performing maintenance on such columns, such as cleaning and replacing bed supports, distributors, nozzles, o-rings and other column components, by the use of a handling device to support, lift, carry and manipulate such column components..
Ge Healthcare Bio-sciences Ab

Quantification of impurities for release testing of peptide products

The present invention relates to a method for the quantitative determination of an impurity present in a peptide product, wherein the impurity cannot be separated from other impurities or the main product. The method particularly involves the use of high resolution mass spectrometry (ms) detection with or without high performance liquid chromatography (hplc).
Sanofi-aventis Deutschland Gmbh

Vial rack for liquid chromatography having draining means

The present invention provides a rack for holding sample vials comprising: (i) a solid base; (ii) an upwardly extending wall around the perimeter of said base; (iii) a plurality of means for supporting a plurality of vials, each means for supporting one vial; and (iv) a means for draining liquid from said rack.. .
Euro-celtique S.a.

Chromatography pipette tip

chromatography pipette tip having a first vessel and a second vessel which is open at two opposite ends and which, in each instance, has a bottom orifice via which a sample liquid can either be sucked in or expelled. The two vessels are fluidically and sealably connected to one another.
Cybio Ag

Compression connection

A chromatography and fluidic device with connections capable of automated component changing, diagnostic leak and current sensing. The chromatography-electrospray device contains a chromatography column, a pre-column, a spray emitter, or other fluidic component imbedded within one or more inserts.
Corsolutions Llc

Use of stationary phase comprising fibril cellulose in separation methods

Separation methods based on electrophoresis or chromatography that use a stationary phase including fibril cellulose.. .
Upm-kymmene Corporation

Industrial process for preparation of 1,2-dihydroquinoline derivative or a salt thereof, and intermediate for preparation thereof

Or a salt thereof in the presence of a base. The method is a method for producing a 1,2-dihydroquinoline derivative having glucocorticoid receptor binding activity or a salt thereof.

High purity lipopeptides

The invention discloses highly purified daptomycin and to pharmaceutical compositions comprising this compound. The invention discloses a method of purifying daptomycin comprising the sequential steps of anion exchange chromatography, hydrophobic interaction chromatography and anion exchange chromatography.
Cubist Pharmaceuticals, Inc.

System for separating a cell sample by centrifugation and column chromatography while maintaining sterility

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.. .
Miltenyi Biotech Gmbh

Chromatography media and devices

chromatography devices contain chromatography media and methods of making and methods of using chromatography devices. Chromatography devices enable a more efficient, productive and/or environmentally friendly chromatographic operation due to one or more of the following advantages over conventional chromatographic operations: elimination of a device packing step by the user; elimination of clean-in-place (cip) steps; elimination of clean-in-place (cip) steps utilizing sodium hydroxide solution; elimination of any validation steps by the user; and use of a chromatography device comprising biodegradable material.
W.r. Grace & Co. Conn.

Hybrid gradient delivery system and operation

A liquid chromatography system includes a gradient proportioning valve in fluidic communication with sources of solvent. From the solvent sources, the gradient proportioning valve produces a low-pressure gradient stream.
Waters Technologies Corporation

Tagatose production from deproteinized whey and purification by continuous chromatography

Disclosed is a process for the production of d-tagatose from deproteinized whey or whey permeate containing lactose after acid hydrolysis to provide a hydrolysate comprising 1 equivalent of d-glucose and 1 equivalent of d-galactose for each unit of lactose converted. More particularly, the invention relates to a process for the isomerization of d-galactose to d-tagatose and the use of a simplified separation scheme based on simulated moving bed (smb) separation.
Orochem Technologies, Inc.

Process for refining glyceride oil and purifying triglyceride oil obtained by such process

The present invention relates to a process for refining glyceride oil by size exclusion chromatography to obtain a triglyceride enriched fraction. The process comprises passing a glyceride oil through a size exclusion column packed with porous particles having a mass weighted mean particle size of 20 to 1,000 μm and an average pore size of 10 to 150 a without using any solvent and collecting an eluate fraction enriched in triglyceride.

Method for obtaining a dialyzable leukocyte extract

The present invention relates to a method for producing a transfer factor. The method comprises the following steps: freezing and thawing of peripheral-blood leukocytes, dialysis, tangential ultrafiltration, identification and quantification using high-resolution, molecular-exclusion liquid chromatography, and in vitro biological validation.

Method and system for analyzing protein or peptide

A peptide is cleaved at various bonding sites into oligopeptides or similar fragments by digestion using proteinase k (s3). The obtained fragments are separated according to their kinds by reversed-phase chromatography and fractionated (s4), and each fragment is subjected to mass spectrometry to determine its mass (s6).

Porous composite particulate materials, methods of making and using same, and related apparatuses

In an embodiment, a porous composite particulate material includes a plurality of composite particles. Each composite particle includes an acid-base-resistant core particle at least partially surrounded by one or more layers of acid-base-resistant shell particles.

Colorant compounds derived from genipin or genipin containing materials

The present disclosure provides colorant compounds and methods of isolation of the colorant compounds derived from a reaction of genipin and an amine. The colorant compositions comprise purified compounds (e.g., a purified polymer or a purified dimer) obtained from multiple fractioning by chromatography of the reaction resulting material.

Methods of producing competitive aptamer fret reagents and assays

Methods are described for the production and use of fluorescence resonance energy transfer (fret)-based competitive displacement aptamer assay formats. The assay schemes involve fret in which the analyte (target) is quencher (q)-labeled and previously bound by a fluorophore (f)-labeled aptamer such that when unlabeled analyte is added to the system and excited by specific wavelengths of light, the fluorescence intensity of the system changes in proportion to the amount of unlabeled analyte added.

Micro-sampling for aquatic chemical analysis

The current invention describes in vivo and vitro (cultured) sampling technologies that allow direct temporal and spatial sampling from living ecosystems such as those associated with marine ecology. The optional use of parallel sampling methods, observatory design, provides for the ability to measure the response of individual organisms to a variety of both biotic and abiotic stresses.

Structured silicon particles

A composite particle is provided. The particle comprises a first particle component and a second particle component in which: (a) the first particle component comprises a body portion and a surface portion, the surface portion comprising one or more structural features and one or more voids, whereby the surface portion and body portion define together a structured particle; and (b) the second component comprises a removable filler; characterised in that (i) one or both of the body portion and the surface portion comprise an active material; and (ii) the filler is contained within one or more voids comprised within the surface portion of the first component.

Ion exchange membrane chromatography

Methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a ph sufficiently distinct from the pi of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and at least one contaminant, (b) overloading the ion exchange membrane such that at least one contaminant remains bound to the membrane while the polypeptide of interest is primarily in the effluent; (c) collecting the effluent from the ion exchange membrane comprising the polypeptide of interest; (d) subjecting the membrane effluent comprising the polypeptide of interest to a purification step of similar charge as the previous membrane, and (e) recovering the purified polypeptide from the effluent of the charged ion exchange chromatography purification step.. .

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Chromatography topics: Chromatography, Chromatograph, Antibodies, Mass Spectrometry, Spectrometry, Quantitative, High Resolution, Switching Valve, Cross Section, Protein A Affinity Chromatography, Amino Acid, Silica Gel, Carboxylic Acid, Antifungal, Isoleucine

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