|| List of recent Cells-related patents
|Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same|
The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Temporal regulation of gene expression by micrornas|
This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant mirna genes having novel circadian expression patterns that are useful for designing recombinant dna constructs for temporally specific expression of at least one gene.
|Nucleic acid molecules encoding enzymes that confer disease resistance in jute|
The invention further relates to vectors, host cells, seeds, and plants comprising such a nucleic acid molecule. One aspect of the invention is an isolated antibody or antigen binding fragment thereof that specifically binds to a polypeptide molecule of the present invention.
Provided are methods for reducing levels of cyanogenic glycosides and improving protein content in plants, as well as plants containing reduced levels of cyanogenic glycosides and improved protein content. In one aspect, such methods comprise, and such plants are created via, tissue-specific expression of a storage protein such as hydroxynitrile lyase in the apoplastic space of cells of the roots and tubers of such plants..
|Antibody producing non-human mammals|
Described are transgenic, non-human animals comprising a nucleic acid encoding an immunoglobulin light chain, whereby the immunoglobulin light chain is human, human-like, or humanized. The nucleic acid is provided with a means that renders it resistant to dna rearrangements and/or somatic hypermutations.
|Transgenic birds that produce chimeric human immunoglobulins|
The invention relates to transgenic birds capable of producing chimeric immunoglobulins, with a combination of human and avian sequence, in their b cells. In some embodiments, the birds are chickens.
|System and method of presenting media content|
A method includes monitoring media content at media content locations to identify media content items. The media content locations are identified in social network data stored by a set-top box device.
|System and method of presenting media content|
A method includes monitoring media content at media content locations to identify media content items. The media content locations are identified in social network data stored by a set-top box device.
|Semiconductor memory devices including separately disposed error-correcting code (ecc) circuits|
A semiconductor memory device may comprise: at least one bank, each of the at least one bank including a plurality of memory cells; an error-correcting code (ecc) calculator configured to generate syndrome data for detecting an error bit from among parallel data bits read out from the plurality of memory cells of each of the at least one bank; an ecc corrector separated from the ecc calculator, the ecc corrector configured to correct the error bit from among the parallel data bits by using the syndrome data and configured to output error-corrected parallel data bits; and/or a data serializer configured to receive the error-corrected parallel data bits and configured to convert the error-corrected parallel data bits into serial data bits.. .
|Memory devices that perform masked write operations and methods of operating the same|
A method of operating a memory device includes: generating an internal read command in response to a received masked write command, the internal read command being generated one of (i) during a write latency associated with the received masked write command, (ii) after receipt of a first bit of masked write data among a plurality of bits of masked write data, and (iii) in synchronization with a rising or falling edge of a clock signal received with an address signal corresponding to the masked write command; reading, in response to the internal read command, a plurality of bits of data stored in a plurality of memory cells, the plurality of memory cells corresponding to the address signal; and storing, in response to an internal write command, the plurality of bits of masked write data in the plurality of memory cells.. .
|Memory device for performing error correction code operation and redundancy repair operation|
Provided are a memory device and a memory module, which perform both an ecc operation and a redundancy repair operation. The memory device repairs a single-bit error due to a ‘fail’ cell by using an error correction code (ecc) operation, and also repairs the ‘fail’ cell by using a redundancy repair operation when the ‘fail’ cell is not repairable by the ecc operation.
|Adaptive raid for an ssd environment|
A system and method for adaptive raid geometries. A computer system comprises client computers and data storage arrays coupled to one another via a network.
|Techniques for reducing power-down time in non-volatile memory devices|
A method includes, in a memory including analog memory cells, storing first data in a group of the memory cells using a first type of storage command that writes respective analog values to the memory cells in the group. Second data is stored in the memory cells in the group, in addition to the first data, using a second type of storage command that modifies the respective analog values of the memory cells in the group.
|Memory device and memory system including the same, and operation method of memory device|
A memory device includes a memory cell array having a plurality of memory cells, a storage unit suitable for storing a fail address corresponding to a fail memory cell in the memory cell array, an available storage capacity determination unit suitable for generating available capacity information indicating an available storage capacity in the storage unit, and an output circuit suitable for outputting the available capacity information.. .
|Assigning method, recording medium, information processing apparatus, and analysis system|
A method includes: calculating a position-dependent processing load to be caused by a process that depends on a position of a cell, for each of cells into which a spatial area specified as a target of an analysis to be executed by processing devices that are included in a first number of processing devices and synchronize for each unit in time domain is divided; and assigning the cells to a second number of processing devices which are part or all of the first number of processing devices, under a condition where a criterion determined with respect to uniformity in a total processing time among processing devices used for the analysis is met. The total processing time of each processing device includes a length of time to be taken to execute each process depending on the position of each of cell(s) to be assigned to this processing device..
|Method and system for spatio-temporal sensor selection|
A computer implemented method, computer program product and computer system for sensor selection. The computer system can run the computer program to execute the method by dividing a two-dimensional area into cells, wherein the cells are arranged in a grid; receiving a selection trigger for a subset of cells of the grid, wherein at least one cell of the subset has at least one sensor and the at least one cell has a sampling frequency associated; determining a set of constraints for the at least one sensor; selecting the at least one sensor if the at least one sensor complies with the set of constraints; and calculating a sampling frequency of the at least one sensor dependent on the sampling frequency of the at least one cell..
|Resilient interpositional arthroplasty device|
This disclosure is directed to a resilient interpositional arthroplasty implant for application into a joint to pad cartilage defects, cushion, and replace or restore the articular surface, which may preserve joint integrity, reduce pain and improve function. The implant may endure variable joint compressive and shear forces and cyclic loads.
|Combined modality treatment systems, methods and apparatus for body contouring applications|
Systems and methods that enable delivery of radiofrequency energy and cryotherapy applications to adipose tissue for reduction and contouring of body fat are described herein. Aspects of the disclosure are directed to methods for reducing surface irregularities in a surface of a subject's skin resulting from an uneven distribution of adipose tissue in the subcutaneous layer.
|Pasteurisation process for microbial cells and microbial oil|
An improved pasteurisation protocol for pasteurising microbial cells is disclosed. The protocol has three stages, a first heating stage, a second plateau stage at which the cells are held at a (maximum and) constant temperature, and a third cooling stage.
|Vulcanized rubber, method of manufacturing same and tire|
The present invention provides a vulcanized rubber capable of improving ice performance of the tire, in particular a vulcanized rubber which is made by vulcanizing a rubber component and has cells with an aspect ratio of 1-30 of which at least a part of a wall surface is hydrophilic.. .
|Compositions and methods for reprogramming eukaryotic cells|
The present invention relates to methods for changing the state of differentiation of a eukaryotic cell, the methods comprising introducing mrna encoding one or more reprogramming factors into a cell and maintaining the cell under conditions wherein the cell is viable and the mrna that is introduced into the cell is expressed in sufficient amount and for sufficient time to generate a cell that exhibits a changed state of differentiation compared to the cell into which the mrna was introduced, and compositions therefor. For example, the present invention provides mrna molecules and methods for their use to reprogram human somatic cells into pluripotent stem cells..
|Cholecystokinin b receptor (cckbr) mini-promoters|
Isolated polynucleotides comprising a cckbr mini-promoters are provided. The mini-promoter may be operably linked to an expressible sequence, e.g.
|Delivering functional nucleic acids to mammalian cells via bacterially-derived, intact minicells|
Intact bacterially derived minicells containing functional nucleic acids or plasmids encoding functional nucleic acids can reduce, in targeted mammalian cells, drug resistance, apoptosis resistance, and neoplasticity, respectively. Methodology that employs minicells to deliver functional nucleic acids, targeting the transcripts of proteins that contribute to drug resistance or apoptosis resistance, inter alia, can be combined with chemotherapy to increase the effectiveness of the chemotherapy..
|Substituted 2-imidazolidinones and 2-imidazolones and their use in the treatment of cancer|
Compounds of formula (i) wherein r1, r4, r8, x and y as defined herein are provided as useful for the inhibition of certain types of cancer cells, amongst others, breast cancer cells, or for the manufacture of anti-cancer agents.. .
|Isoxazole treatments for diabetes|
The present invention relates to compounds and methods for inducing synthesis and secretion of insulin from pancreatic beta cells. The methods may take place in vitro, ex vivo such as in isolates from adult mammalian tissue, or in vivo.
|Bis-(aryl/heteroaryl)-methylene compounds, pharmaceutical compositions containing same and their use for treating cancer|
The present disclosure relates to novel compounds having vitamin d receptor agonist and histone deacetylase inhibitory efficacy as well as methods for reducing or inhibiting the proliferation of cancer cells or for treating cancer.. .
|Design, synthesis and evaluation of procaspase activating compounds as personalized anti-cancer drugs|
Compositions and methods are disclosed in embodiments relating to induction of cell death such as in cancer cells. Compounds and related methods for synthesis and use thereof, including the use of compounds in therapy for the treatment of cancer and selective induction of apoptosis in cells are disclosed.
|Composition comprising a glucocorticoid and a thiazolidinedione for inducing compelte adipogenic differentiation of mammalian stem cells|
The present invention relates to the provision of a composition comprising a glucocorticoid and a thiazolidinedione for inducing adipogenic differentiation, thereby successfully generating functional stem cells, from non-differentiated embryonic or adult stem cells originating from humans or other mammals, special preference being given to human mesenchymal stem cells. The preferred glucocorticoid is dexamethasone and the preferred thiazolidinedione is rosiglitazone.
|Therapeutic uses of ectoine|
The invention relates to a composition containing ectoine, hydroxyectoine and/or a salt, ester or amide of these compounds for the suppression of anti-apoptotic signals to neutrophil granulocytes and other cells taking part in inflammations. The delayed apoptosis of the neutrophils is a main component for various types of inflammation.
|Methods and compositions related to glucocorticoid receptor antagonists and breast cancer|
Embodiments of the invention are directed to methods of determining the prognosis of a breast cancer patient by evaluating the activity of the glucocorticoid receptor in tumor cells. Other embodiment include methods of treating breast cancer cells, particularly, chemo-resistant cells, with a glucocorticoid receptor antagonist and an anticancer agent or compound..
|Compounds and compositions for use in the prevention and treatment of inflammation-related disorders, pain and fever, skin disorders, cancer and precancerous conditions thereof|
The present invention provides novel compounds and pharmaceutical compositions for the prevention and/or treatment of cancer and precancerous conditions thereof, for the treatment of pain and fever, for the treatment of skin disorders, and for treating and/or preventing inflammation-related diseases and/or cardiovascular diseases. The compounds of the invention also have analgesic properties and anti-platelet properties.
|Anti-cancer compositions containing wnt decoy receptor|
The present invention relates to a composition for preventing or treating cancer comprising wnt decoy receptor. The composition of the present invention or the expression product thereof inhibits cancer generation, growth, proliferation and metastasis, and induces apoptosis of cancer cells, by binding to wnt ligand and blocking ligand-receptor interactions, therefore may be effectively used as an anti-cancer agent..
|High mast2-affinity polypeptides and uses thereof|
The invention relates to polypeptides containing a cytoplasmic domain ending with a mast-2 binding domain, from 11 to 13 residues, the first two residues of which are s and w, and the last four residues of which are q, t, r and l, said polypeptides presenting a high affinity for the pdz domain of the human mast2 protein. The invention also relates to polynucleotides, vectors, lentiviral particles, cells as well as compositions comprising the same.
|Use of an active biological substance in abnormal cellular and viral membrane physiologies|
An active biological substance is disclosed for use in abnormal cellular and viral membrane physiologies in human and mammal organisms. The active substance has diagnostic and/or therapeutic properties and contains or consists of at least one component selected from the group of substances including: histones, covalently modified histones, histone-like polypeptides, biologically active histone sequences and histone-like polypeptides as agents for stopping the supply to solid tumours over their blood vessels, for killing cells infected by virus and for killing tumour cells with disturbed lipid asymmetry..
|Compositions and methods for selective delivery of oligonucleotide molecules to cell types|
The invention provides a conjugate comprising (i) a nucleic acid which is complementary to a target nucleic acid sequence and which expression prevents or reduces expression of the target nucleic acid and (ii) a selectivity agent which is capable of binding with high affinity to a receptor which can be internalised by the cell in response to the binding of said selectivity agent. The conjugates of the present invention are useful for the delivery of the nucleic acid to a cell of interest and thus, for the treatment of diseases which require a down-regulation of the protein encoded by the target nucleic acid as well as for the delivery of contrast agents to the cells for diagnostic purposes..
|Methods related to the treatment of neurodegenerative and inflammatory conditions|
The invention includes methods of neuroprotection, inducing release of neurotrophic factors, inhibiting the over-activation of innate immune cells, attenuating the toxin-induced death and/or damage of tissues, reducing inflammation, treating an inflammation-related condition, and inhibiting nadph oxidase, that includes contacting or administering an effective amount of at least one compound of the invention that include: valproic acid, sodium butyrate, and salts thereof; opioid peptides; a peptide comprising the tripeptide ggf; and morphinans, such as naloxone, naltrexone, 3-hydroxy-morphinan and dextromethorphan.. .
|Photonic blood typing|
Photonic devices, systems, and methods for detecting an analyte in a biological solution (e.g., whole blood) are provided. Representative photonic devices are optical ring resonators having nanoscale features and micron-sized diameters.
|Engineering a heterogeneous tissue from pluripotent stem cells|
Aspects of the present disclosure are directed to methods and compositions for the production of heterogeneous tissue from human induced pluripotent stem (hips) cells.. .
|Methods and kits for detecting ige-expressing b cells|
The invention relates to the field of medical diagnostics. In particular, it relates method and kits for identification and classification of ige-related diseases, e.g.
|Detection, isolation and analysis of rare cells in biological fluids|
The invention provides a method for isolating or enriching a rare cell from a biological fluid of a mammal employing an antibody that binds a cell-surface antigen of the rare cell. The immobilized antibody is incubated with a sample of biological fluid that includes the rare cells and a plurality of other cells so as to form an antibody-rare cell complex.
|Assessment of oocyte competence by detecting spsb2 and/or tp53i3 gene expression|
Methods are provided for evaluating an oocyte for fertilization and implantation comprising analysis of gene expression in cumulus cells wherein the detected genes or polypeptides include at least spsb2 and tp53i3. For example, methods are provided for determining whether a cumulus cell expresses, or does not express, one or more of a group of markers identified as differently expressed between cumulus cells associated with chromosomally normal oocytes and cumulus cells associated with chromosomally abnormal oocytes.
|Methods and compositions for sorting and/or determining organisms|
This invention is directed to methods and compositions for sorting and/or determining microscopic organisms or cells. The methods and compositions are directed to the use of molecular probes to selectively stain the organisms or cells in combination with the use of binding partners to selectively immobilize the stained organisms or cells to a solid carrier.
|Femto cell visitation history for location based services|
Collection and communication of data are provided on handset attachment procedure, or visitation, to one or more femto cells to provide location information. A mobility component receives actual subscriber and femto access point (ap) attachment signaling and extracts visitation data that facilitates generation of real-time or historical visitation reports.
|Method and device for sending aggregated cqi|
A method and device for sending an aggregated cqi are provided by the disclosure. The method includes a terminal side receiving csi-rs configuration information and/or interference measurement information sent from a base station side, the terminal side determining a sending sequence of aggregated cqi of each aggregated csi-rs configuration resource and/or interference measurement resource according to a receiving sequence of the csi-rs configuration information and/or interference measurement information, wherein each aggregated csi-rs configuration resource and/or interference measurement resource is aggregated of at least one configuration csi-rs configuration resource and/or interference measurement resource corresponding to the csi-rs configuration information and/or interference measurement information, the terminal side sending the aggregated cqi to the base station according to the sending sequence.
|Inter-system interference in communications|
An inter-system interference estimation method is disclosed for both primary and secondary radio systems. In the case of a primary system (such as a gsm system), inter-system interference estimation is based on a comparison of cell level measurement statistics before and after secondary spectrum usage in neighbouring cells.
|Base station, communication system, communication method and storage medium|
A base station includes: a reception unit for receiving a first adjacent cell list which is a list of cells adjacent to a cell managed by an adjacent base station from the adjacent base station; and a control unit for employing the first adjacent cell list as a second adjacent cell list that is a list of cells adjacent to a cell managed by the base station itself.. .
A communication system includes a communication network including a plurality of communication cells, a plurality of mobile communication devices each operable to connect into the communication network via at least one of the communication cells, and a communication apparatus for providing at least one of the communication cells. The communication apparatus includes first and second base station functions each operable to acquire a congestion status and access control information for the other base station..
A communication system includes a communication network including a plurality of communication cells, a plurality of mobile communication devices each operable to connect into the communication network via at least one of the communication cells, and a communication apparatus for providing at least one of the communication cells. The communication apparatus is operable to provide first and second communication cells via which a mobile communication device can connect to a communication network and to determine whether a mobile communication device is connected to the communication network via the first and/or second communication cells.
|Radio parameter control system, radio parameter control apparatus, radio base station, radio parameter control method and program|
An apparatus for controlling a radio parameter of radio cells, includes: an evaluation targeted radio cell selection unit configured to decide evaluation targeted candidate radio cells based on a change of a connection target radio cell of a radio terminal, which takes a control targeted radio cell as a serving cell or surrounding radio cells of the control targeted radio cell as a serving cell, associated with a change of a connection range of the control targeted radio cell by control of the radio parameter of the control targeted radio cell, and select at least one of the evaluation targeted candidate radio cells as an evaluation targeted radio cell for evaluating the radio parameter of the control targeted radio cell; and a radio parameter control unit configured to control the radio parameter of the control targeted radio cell based on quality of the evaluation targeted radio cell.. .
|Resistive random access memory cells having metal alloy current limiting layers|
Provided are semiconductor devices, such as resistive random access memory (reram) cells, that include current limiting layers formed from alloys of transition metals. Some examples of such alloys include chromium containing alloys that may also include nickel, aluminum, and/or silicon.
|Disk-type microfluid system and method for checking blood status|
A method for checking blood status comprises: a step of supplying blood to the centrifuge container of a disk; a step of rotating the disk to centrifuge the blood into blood cells and blood plasma in the centrifuge container, and detecting the actual moving distance per hour of the blood cells in the centrifuge container; and a step of establishing a first graph which represents the actual moving distance of the blood cells per hour, and a second graph which represents the theoretical moving distance of the blood cells per hour, and thereafter calculating the hematocrit of the blood cells and the viscosity of the blood plasma by comparing the first graph with the second graph.. .
|Modified gene silencing|
This invention relates to methods of controlling gene expression or gene suppression in eukaryotic cells. One aspect of this invention includes modifying the degree of silencing of a target gene by use of a modified suppression element.
|Method and device for cell modification|
The invention relates to a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135-45° to the rotational axis of the centrifugation chamber, wherein the centrifugation chamber comprises at least one input/output port and the cells to be modified are immobilized at the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g. Furthermore, the invention relates to a method for modifying cells comprising the steps—introducing cells in a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135-45° to the rotational axis of the centrifugation chamber wherein and comprising at least one input/output port, —immobilizing the cells on the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g—maintaining the rotation of the rotation of the centrifugation chamber until the cells are modified..
|Recombinase-based logic and memory systems|
The invention provides, inter alia, recombinase-based systems that provide for integrated logic and memory in living cells.. .
|Method for manufacturing in vitro vascularized tissue|
The present invention relates to a method for manufacturing an in vitro vascularized tissue, which enables obtaining the in vitro vascularized tissue by inducing in vitro angiogenesis in a tissue using vascular cells, for use in in vitro research of diseases in vascularized tissues and developing a treatment. The method for manufacturing the in vitro vascularized tissue comprises the steps of: supplying to a container for manufacturing tissue a hydrogel in which vascularized tissue cells are mixed; submerging a collection tip to which vascular cells are coupled into the hydrogel; hardening the hydrogel while the collection tip is submerged; and supplying a cell culture fluid to the upper side of the hydrogel..
|Glucomannan scaffolding for three-dimensional tissue culture and engineering|
The present invention provides a neutralized glucomannan scaffold capable of promoting cell growth and suitable for three-dimensional tissue culture and engineering. The present invention also provides methods for making and degrading the neutralized glucomannan scaffold.
|Mixed population of cells deriving from adipose tissue and methods of isolating and using the same|
Adipose tissue-derived stromal cells and methods of isolating and using the same. In at least one embodiment of isolated adipose tissue-derived stromal cells of the present disclosure, the cells are isolated by performing adipose tissue resection or suction on a mammalian patient, dissecting tissue obtained from said tissue resection or suction and dissociating said tissue into a cell suspension, removing adipocytes from the cell suspension, culturing the adipocyte-depleted cell suspension in egm-2-mv media, and isolating adipose tissue-derived stromal cells secreting vascular endothelial growth factor (vegf), hepatocyte growth factor (hgf), and granulocyte-colony stimulating factor (g-csf)..
|Cell culture substrate comprising a laminin and a cadherin|
The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells.
|Method for producing corneal endothelial cell|
The invention provides a method of efficiently producing corneal endothelial cells, particularly from corneal stroma or ips cell-derived neural crest stem cells, a method of producing corneal endothelial cells stably in a large amount by inducing more efficient differentiation of stem cells into corneal endothelial cells, and a medicament containing corneal endothelial cells. The method of inducing differentiation of stem cells into corneal endothelial cells includes a step of culturing the stem cells in a differentiation induction medium containing a gsk3 inhibitor (preferably a gsk3β inhibitor) and retinoic acid, with the differentiation induction medium preferably further containing one or more of tgfb2, insulin, a rock inhibitor, and the like..
|Reprogramming t cells and hematopoietic cells|
Methods and compositions relating to the production of induced pluripotent stem cells (ips cells) are disclosed. For example, induced pluripotent stem cells may be generated from cd34+ hematopoietic cells, such as human cd34+ blood progenitor cells, or t cells.
|Aligned nanofibers and related methods of use|
The present invention relates to nanofibers. In particular, the present invention provides aligned nanofiber bundle assemblies.
|Isolated naive pluripotent stem cells and methods of generating same|
Provided is an isolated human naive pluripotent stem cell (psc), wherein: (i) when the naive psc is a female psc, then said naive female psc has two unmethylated alleles of an x-inactive specific transcript (xist) gene; and (ii) when said naive psc is a male psc, then said naive male psc has an unmethylated allele of said xist gene. Also provided is a culture medium which comprises an erk1/2 inhibitor, a gsk3beta inhibitor, a p38 inhibitor, a jnk inhibitor, a stat3 activator and at least one agent selected from the group consisting of: bfgf, tgfbeta 1, a pkc inhibitor, a rock inhibitor and a notch inhibitor; or at least agent selected from the group consisting of: a tgfr inhibitor, a fgfr inhibitor, a pkc inhibitor, a rock inhibitor and a notch inhibitor..
|Microcarriers for stem cell culture|
We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm.
|Engineered stem cells and their therapeutic use|
The present invention relates to mesenchymal stem cells (msc) genetically modified to express sst2 or parts thereof for use in the treatment of airway immune inflammatory and lung diseases, wherein said mesenchymal stem cells are not human embryonic stem cells. The present invention also relates to pharmaceutical compositions comprising said mesenchymal stem cells..
|Method for isolating cells and bioparticles|
The invention relates to a simple and inexpensive method for simultaneous isolation of cells and/or bioparticles from complex fluids that differ in one or more surface properties (e.g. Cell sub-populations).
|Highly efficient gas permeable devices and methods for culturing cells|
This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling.
|Polymer microfilters, devices comprising the same, methods of manufacturing the same, and uses thereof|
A microfilter having a hydrophilic surface and suited for size-based capture and analysis of cells, such as circulating cancer cells, from whole blood and other human fluids is disclosed. The filter material is photo-definable, allowing the formation of precision pores by uv lithography.
|Disposable cartridge for erythrocyte mechanical fragility test|
A disposable cartridge for use in red blood cell mechanical fragility profiling, comprising a stressing section with a lysing chamber in which the sample can receive multiple increments of mechanical stress to cause lysis, and a detection section with a transparent region that at least sometimes can have a thickness suitable to take optical readings that can directly or indirectly reflect a fraction/proportion of cells lysed after each increment of the stress, whereby said region can capture at least a representative portion of the sample that received each increment of stress. In some embodiments, the stressing section and the detection section are the same, and/or the transparent region is flexible or otherwise compressible, and/or the stress is ultrasonic.
|Isoprene synthase variants with improved solubility for production of isoprene|
The present invention provides methods and compositions of variant polypeptides having isoprene synthase activity with improved solubility. In particular, the present invention provides isoprene synthase variant for increased isoprene production in recombinant host cells..
|Novel lipolytic enzyme lip2|
The present invention provides a novel nucleic acid sequence, designated lip2, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same..
|Use of recombinant type iii "polyketide synthases" (pks iii) of marine brown algae|
The present invention relates to a method for producing polyphenol compounds, i.e. Phloroglucinol or one of its derivatives, with a polyketide synthase of type iii (pksiii) from a brown marine alga.
|Tailored oils produced from recombinant heterotrophic microorganisms|
Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-acp synthase enzyme, a fatty acyl-acp thioesterase, a fatty acyl-coa/aldehyde reductase, a fatty acyl-coa reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants..
|Cd40l expressing mammalian cells and their use|
Herein is reported a co-cultivation system for co-cultivating a pool of rabbit b-cells or single deposited rabbit b-cells wherein cells cd40l expressing cho cells are used as feeder in the presence of il-2 and il-21.. .
|Inducible eukaryotic expression system|
Compositions and methods for the inducible expression of genes in eukaryotic cells are provided. Expression of a nucleotide sequence of interest encoding a protein of interest is controlled by a regulatory fusion protein that consists of a transcription blocking domain and a ligand-binding domain.
|Protein production method using transformed plant cells|
An object of this invention is to produce a protein by efficiently culturing plant cells while escaping mrna translational repression in cultured plant cells under stress caused by lack of conditions essentially required for the growth (e.g., nutrient-starvation stress and hypoxic stress). This invention provides a method for escaping translational repression of a protein encoded by mrna, the repression being induced by stress due to absence of conditions required for the growth, the method comprising the step of culturing a plant cell transformed with a recombinant dna molecule encoding mrna containing 5′ utr defined in (a) or (b) below, the culturing being carried out under stress due to absence of conditions required for the growth: (a) 5′ utr having a base sequence of seq id no: 1, 2, 3, 4, 5, or 6; or (b) 5′ utr having the base sequence of the 5′ utr of (a) in which one or more bases are replaced, deleted, or added, and which escapes translational repression induced by stress due to absence of conditions required for the growth..
|Expression of biologically active proteins in a bacterial cell-free synthesis system using bacterial cells transformed to exhibit elevated levels of chaperone expression|
The present disclosure describes methods and systems for improving the expression of a properly folded, biologically active protein of interest in a cell free synthesis system. The methods and systems use a bacterial cell free extract having an active oxidative phosphorylation system, and include an exogenous protein chaperone.
|Rapid measurement of formed blood component sedimentation rate from small sample volumes|
Devices and methods are described for measuring formed blood component sedimentation rate. Some of the methods may use (1) centrifugal techniques for separating red blood cells from plasma and (2) video and/or still imaging capability.
|Capturing of cell fluid and analysis of its components under observation of cells and instruments for the cell fluid capturing and the analysis|
A method captures cellular components from a single cell and performs mass spectrometry on the components. The method includes inserting a nanospray ionization capillary tip into a specific region of the cell under observation with a microscope.
|Coated fiber scaffold for three dimensional cell culture of neural cells|
The invention relates to a biocompatible scaffold for three dimensional cultivation of cells, said scaffold comprise one or more fibers randomly oriented to form a scaffold with open spaces for cultured cells. The one or more fibers are also coated with a bio-active coating and have a diameter of 100-3000 nm..
|Method to generate dopaminergic neurons from mouse and human cells|
The present invention relates to a method for reprogramming a differentiated non neuronal cell into a dopaminergic neuron comprising the step of inducing the expression in the differentiated non neuronal cell of at least the protein encoded by the mash1 human gene or orthologues thereof and the protein encoded by the nurr1 human gene or orthologues thereof, expression vectors, reprogrammed dopaminergic neuron and uses thereof.. .
|Buffered histology and cytology stains|
A composition for use in staining histological and cytological tissue and cell samples which includes a buffer component in a staining solution. Specifically, the invention relates to stains and methods of producing stains for histological and cytological microscopic evaluation of tissue and cells.
|System for detecting rare cells|
This disclosure provides a system for detecting rare cells. The system includes a substrate, an extension coupled to the substrate and extending outwardly from the substrate, and a functionalized graphene oxide disposed on the extension.
|Alpha toxin detection of gpi anchored proteins|
The present invention relates to a method for the purification, concentration and identification of glycosylphosphatidylinositol anchored proteins (gpi-aps) from a biological sample (cells, tissues and/or blood/serum) in a patient or subject, including a human patient or subject. A new method to separate gpi-anchored glycoproteins, a class of glycoproteins found in all animal cells and fluids including serum, from other glycoproteins and proteins for the purpose of identifying potential biomarkers for various diseases, including cancer, especially breast cancer, vaginal cancer, endometrial cancer, uterine cancer, cervical cancer, pancreatic cancer and prostate cancer.
|Assessment of pml risk and methods based thereon|
The invention provides a method of assessing the risk of occurrence of progressive multifocal leukoencephalopathy (pml) in a subject as well as a method of stratifying a subject undergoing α4-integrin and/or vla-4 blocking agent treatment for suspension of the treatment and a method of stratifying a subject undergoing highly active antiretroviral therapy (haart) for alteration of haart. These methods comprise detecting the level of p-selectin glycoprotein ligand-1 (psgl-1) expressing t cells in a sample from the subject..
|Method for the preparation of catalyst-coated membranes method for the preparation of catalyst-coated membranes|
The present invention is directed to a method for preparing an integral 3-layer catalyst-coated membrane (ccm) for use in electrochemical cells, e.g. Pem (polymer-electrolyte membrane) fuel cells.
|Electrode including current collector with nano-scale coating and method of making the same|
Current collectors and methods are provided that relate to electrodes that are useful in electrochemical cells. The provided current collectors include a metallic substrate, a substantially uniform nano-scale carbon coating, and an active electrode material.
A battery module includes a plurality of battery cells arranged in a same direction and a same orientation; and a housing portion disposed on the outer surfaces of the plurality of battery cells. In the battery module, the housing portion includes a reinforcing plate positioned between at least two of the battery cells.
|Battery system and method of producing an electrically conductive connection between a cell connector and an electronic unit of a battery system|
A battery system and a method for producing an electrically conductive connection. The battery system includes a plurality of cells that are arranged in at least in one stack, an electronic unit, and a conductor.
|Battery pack of compact structure|
Disclosed herein is a battery pack including a battery module including a cell module stack having a structure in which a plurality of cell modules, each of which includes a battery cell mounted in a cartridge, is vertically stacked, a lower end plate to support a lower end of the cell module stack, an upper end plate to fix an uppermost cartridge of the cell module stack disposed on the lower end plate, and a voltage detection assembly to detect voltages of the battery cells, a box type pack case in which the battery module is mounted, a pack cover coupled to the pack case, and fastening extension members protruding upward from the battery module to couple the battery module to the pack case and the pack cover.. .
|Electrolyte fluid metering device for lithium cells|
A battery system includes at least one lithium cell with an electrolyte having at least one polymer which is configured to be impregnated with an electrolyte fluid. In order to increase the capacity, the life and the safety of the battery system, the battery system further includes at least one electrolyte fluid metering device, by which at least one component of the electrolyte fluid can be supplied to the lithium cell and/or by which electrolyte fluid can be discharged from the lithium cell..