|| List of recent Cells-related patents
This document provides methods and materials related to transcription terminators. For example, methods and materials related to transcription terminators and nucleic acid molecules (e.g., vectors and constructs) that contain a nucleic acid sequence that encodes a polypeptide operably linked to a transcription terminator are provided.
|Cells producing glycoproteins having altered glycosylation patterns and method and use thereof|
The disclosure relates to the field of glyco-engineering, more specifically, to eukaryotic cells wherein both an endoglucosaminidase and a glycoprotein are present. These cells can be used to deglycosylate or partly deglycosylate the (exogenous) glycoprotein, in particular, without the need for adding an extra enzyme.
|Methods of modulating stomata conductance and plant expression constructs for executing same|
Plant expression construct are provided. According to an embodiment, the plant expression construct comprises a nucleic acid sequence encoding a hexokinase under a transcriptional control of a guard cell-specific cis-acting regulatory element.
|Family of pesticidal proteins and methods for their use|
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided.
|Soybean atps promoter and its use in constitutive expression of transgenic genes in plants|
The invention relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean atp sulfurylase (atps) and fragments thereof and theft use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-independent or constitutive manner in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same..
|Genes for enhancing nitrogen utilization efficiency in crop plants|
The invention provides isolated nue (nitrogen utilization efficiency) nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering nitrogen utilization and/or uptake in plants.
|Production and use of rat spermatogonial stem cell lines|
A spermatogonial stem cell line that is derived from testes of rats characterized by a desirable genetic background can serve as a source for cells to transplant into male-sterile recipient animals that are immuno-compatible with the spermatogonial line. Rat cells thus transplanted readily develop into fertilization-competent, haploid male gametes, with little or no endogenous sperm competition generated by the testes of the male-sterile recipient.
|Apparatus and method for designing an integrated circuit layout having a plurality of cell technologies|
A system and method of designing a layout for a plurality of different logic operation (lop) cell technologies includes defining a priority for each lop cell technology in the plurality of different lop technologies and forming a layout of the plurality of different lop cells for formation on a substrate with at least some of the lop cells of higher priority lop technologies overlapping lop cells of lower priority lop technologies. The system can include a processor coupled to memory where stored code defines the priority for each different cell technology in the plurality of lop cells and (when the code is executed) the processor forms the layout of a plurality of different lop cells.
|Method for correcting electronic proximity effects using the deconvolution of the pattern to be exposed by means of a probabilistic method|
A method of lithography by radiation having critical dimensions of the order of some ten nanometers makes it possible to carry out the correction of the proximity effects by joint optimization of the dose modulation and geometric corrections. Accordingly, a deconvolution of the pattern to be etched is carried out by an iterative procedure modeling the interactions of the radiation with the resined support by a joint probability distribution.
|Method and apparatus for broadcasting scan patterns in a scan-based integrated circuit|
A broadcaster, system, and method for reducing test data volume and test application time in an ate (automatic test equipment) in a scan-based integrated circuit. The scan-based integrated circuit contains multiple scan chains, each scan chain comprising multiple scan cells coupled in series.
|Memory controller for micro-threaded memory operations|
A micro-threaded memory device. A plurality of storage banks are provided, each including a plurality of rows of storage cells and having an access restriction in that at least a minimum access time interval must transpire between successive accesses to a given row of the storage cells.
|Marching memory, a bidirectional marching memory, a complex marching memory and a computer system, without the memory bottleneck|
A marching memory is disclosed having an array of memory units. Each memory unit has a sequence of bit level cells.
|Distortion cancellation in 3-d non-volatile memory|
A method in a memory that includes multiple analog memory cells arranged in a three-dimensional (3-d) configuration, includes identifying multiple groups of potentially-interfering memory cells that potentially cause interference to a group of target memory cells. Partial distortion components, which are inflicted by the respective groups of the potentially-interfering memory cells on the target memory cells, are estimated.
|Memory system with a programmable refresh cycle|
A memory system with a programmable refresh cycle including a memory device. The memory device includes refresh circuitry in communication with a memory array and with a memory controller.
|Semiconductor device and method of operating the same|
A semiconductor device includes a memory block including memory cells coupled to bit lines, read/write circuits each including cache latch suitable for temporarily storing data to be stored in the memory cells, wherein the read/write circuits are divided into a plurality of groups and perform a program operation to store the data in the memory cells coupled to the bit lines, and an initialization control unit suitable for initializing the cache latches of the read/write circuits of a group corresponding to the address before the data is input to the cache latches, when a program command and an address are input.. .
|Use of host system resources by memory controller|
A method for data storage includes, in a system that includes a host having a host memory and a memory controller that is separate from the host and stores data for the host in a non-volatile memory including multiple analog memory cells, storing in the host memory information items relating to respective groups of the analog memory cells of the non-volatile memory. A command that causes the memory controller to access a given group of the analog memory cells is received from the host.
|Bioabsorbable biomedical implants|
A bioabsorbable biomedical implant is disclosed. The implant includes a tubular scaffold comprising a plurality of interconnected polymer struts.
|Method and device for producing a bioartificial tissue construct|
The tissue construct with viable cells in an extracellular matrix made of fibrin is produced with a special method, in which a matrix material and cells are shaped into a hollow body, in particular a tubular hollow body, by means of a rotational casting method in a hollow mould (1), the method comprising the following steps: (a) introduction of cells of at least one cell type and/or a fibrinogen preparation into the rotating hollow mould (1) with the aid of an applicator (4), said applicator (4) being displaced along the rotational axis during the introduction and step (a) being performed one or more times; (b) continuation of the rotation process until the fibrinogen solidifies into a dimensionally stable matrix, obtaining a primarily solidified tissue construct; (c) removal of the tissue construct from the mould. The construct can also he obtained in a relatively short time from autologous materials..
|Methods and related compositions for the treatment of cancer|
A method of treatment and/or prevention of cancer comprises administering agents which cause increased intracellular granularity in cancer cells, at least in an amount sufficient to inhibit proliferation of such cells and preferably in an amount sufficient to lead to cancer cell death. The method is particularly directed to refractory cancer, particularly hormone refractory prostate cancer.
|Il-17 binding proteins|
Proteins that bind il-17 and/or il-17f are described along with there use in composition and methods for treating, preventing, and diagnosing il-17 related diseases and for detecting il-17 in cells, tissues, samples, and compositions.. .
|Amino acid sequences directed against il-6r and polypeptides comprising the same for the treatment of il-6r related diseases and disorders|
The invention also relates to nucleic acids encoding such amino acid sequences and polypeptides, to methods for preparing such amino acid sequences and polypeptides, to host cells expressing or capable of expressing such amino acid sequences or polypeptides, to compositions, and in particular to pharmaceutical compositions, that comprise such amino acid sequences, polypeptides, nucleic acids and/or host cells, and to uses of such amino acid sequences or polypeptides, nucleic acids, host cells and/or compositions, in particular for prophylactic, therapeutic or diagnostic purposes.. .
|Plasma protein fractionation by sequential polyacid precipitation|
There is a recognized need for novel, more simplified, approaches to isolation of plasma from whole blood, as well as a need to isolate cell-free plasma fractions containing different plasma proteins. Methods are divulged for use of aqueous phase systems, formed in blood or blood containing solutions via addition of a single polymer at relatively low concentration, to effect isolation (clarification) of plasma proteins from blood cells.
|Interleukin-2 muteins for the expansion of t-regulatory cells|
Provided herein are il-2 muteins and il-2 mutein fc-fusion molecules that preferentially expand and activate t regulatory cells and are amenable to large scale production. Also provided herein are variant human igg1 fc molecules lacking or with highly reduced effector function and high stability despite lacking glycosylation at n297.
|Cell killing fusion peptide exhibiting tumor cell-specific necrosis induction and tumor regression|
A cell-killing peptide, more specifically a cell-killing ckp fusion peptide (ctd7:ckp) is disclosed, wherein a cell-killing peptide (ckp) comprising 10 amino acids in mtd of noxa protein causing cell death, and 7 amino acids targeting a cancer cell are fused. The cell-killing ckp fusion peptide induces strong cell necrosis at various cancer cell lines (hela, hct116, mcf-7, a549, bjab, ct26, pc3 and the like) and shows strong tumor regression effect at a mouse tumor model using experimental animals, but does not show apoptosis at normal cells.
|Integration of molten carbonate fuel cells in fischer-tropsch synthesis|
In various aspects, systems and methods are provided for integration of molten carbonate fuel cells with a fischer-tropsch synthesis process. The molten carbonate fuel cells can be integrated with a fischer-tropsch synthesis process in various manners, including providing synthesis gas for use in producing hydrocarbonaceous carbons.
|Compositions and methods for the suppression of target polynucleotides from lygus|
Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the lygus genus, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant.
|Method for reducing expression of downregulated in renal cell carcinoma in malignant gliomas|
The present invention relates to novel compositions and therapeutic methods for the treatment of cancer, in particular malignant glioma. The compositions include antisense oligonucleotides or rnas or vectors encoding them which reduce expression of downregulated in renal cell carcinoma (drr) in tumor cells, and inhibit malignant glioma cell invasion..
|Pharmaceutical containing ppar-delta agonist|
A preparation containing [3-[2-[4-isopropyl-2-(4-trifluoromethyl)phenyl-5-thiazolyl]ethyl]-5-methyl-1,2-benzisoxazol-6-yl]oxyacetic acid, [4-[3-[2-(4-trifluoromethyl)phenyl-4-isopropyl-5-thiazolyl]propionyl]-2-methylphenoxy]acetic acid or [4-[3-[2-(2-hydroxy-4-chlorophenyl)-5-isopropyl-4-oxazolyl]propionyl]-2-methylphenoxy]acetic acid, or a pharmacologically acceptable salt thereof as an active ingredient is used as an agent for promoting proliferation of meibomian gland epithelial cells or corneal epithelial cells, as well as a therapeutic agent for ocular diseases such as meibomian gland dysfunction, dry eye and the like.. .
|Compounds for inhibiting the interaction of bcl2 with binding partners|
The present invention relates to compounds of formula i: in which r1, r2, r3 and r4 are as defined in the summary of the invention. Compounds of formula i are capable of disrupting the bcl-2 interations with proteins containing a bh3 domain.
|Novel piperazines, pharmaceutical compositions and methods of use thereof|
Disclosed are novel piperazine derivatives that act as agonists of the α7 nachr. Also disclosed are pharmaceutical compositions, methods of treating inflammatory conditions, methods of treating cns disorders, methods for inhibiting cytokine release from mammalian cells and methods for the preparation of the novel compounds..
|Methods to modulate acute myeloid leukemia stem/progenitor cell expansion and/or differentiation|
Screening assays to identify compounds that may be useful for inhibiting and/or eliminating aml initiating cells using ahr modulators and/or the compounds of formula i or ii are also disclosed. The use of pharmaceutically acceptable agonists of the ahr for preventing or inhibiting minimal residual disease (mrd) in an aml patient is also disclosed..
|Indole derivatives inhibitors of enzyme lactate dehydrogenase (ldh)|
The present invention encompasses compounds having general formula (i) able to inhibit the lactate production (lactic acid) involved in the angiogenesis of tumoral tissues, in the glycolytic metabolic process of tumoral cells, of immune system cells in asthmatic diseases, in vascular cells in the pulmonary hypertension, in the treatment of chronic back pain or hyperoxaluria, and in the process by which the parasites protozoan causing malaria obtain most of the necessary energy.. .
|Compositions for regenerating defective or absent myocardium|
Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition can also include an extracellular matrix scaffold component of any formulation, and further include added cells, proteins, or other components to optimize the regenerative process and restore cardiac function..
|Functional cell surface display of ligands for the insulin and/or insulin growth factor 1 receptor and applications thereof|
Systems for making, identifying, and selecting recombinant cells that express a ligand for the insulin receptor (ir) or insulin growth factor i (igf-1) receptor are described. In general, libraries of recombinant cells are constructed that are capable of displaying a plurality of ligand molecules on the cell surface.
|Production method of base metal catalyst for exhaust gas purification (as amended)|
To provide a production method of a base metal catalyst for exhaust gas purification and with a catalyst activity, in which a base metal-supported catalyst is uniformly supported by a honeycomb substrate. A production method of a catalyst for exhaust gas purification, comprising the steps of preparing a honeycomb substrate having a plurality of cells, preparing a base metal-supported catalyst comprising a carrier supporting a metal containing a base metal having an exhaust gas purification performance, preparing a slurry having a ph of 7 or more by dispersing the base metal-supported catalyst in an aqueous solution, and introducing the slurry to a partition wall surface of the honeycomb substrate..
|Lighting management in virtual worlds|
A system has a server with a processor, a storage medium and coded instructions executable on the processor causing the server to display a video game to a player operating a computer, the video game comprising a virtual world having objects with surfaces, movable in the space of the virtual world by the player, and one or more light sources, create and manage a voxel grid associated with the player, compute an occupancy value for individual ones of the cells of the grid, compute an illumination value for cells of the grid by consideration of position and direction of the light sources, the occupancy value of the cell, and illumination values of immediately adjacent cells in the voxel grid, and determine brightness for pixels in display of surfaces of objects in frames of the video game based on the illumination values of cells proximate to the surfaces of the objects.. .
|Method for sending data and terminal thereof|
The present invention discloses a method for sending data. The method includes a cell virtual set is established directly or according to signal metric values of neighboring cells in a cell forward access channel state, where the cell virtual set includes at least a serving cell; a common relative grant channel of non-serving cells in the cell virtual set is monitored, and instruction information in the common relative grant channel is read; power for sending data to be sent is adjusted according to the read instruction information, and the data to be sent is sent by using the adjusted power.
|Methods and apparatus for beamforming for femtocells|
Methods and apparatus for beamforming for femtocells, such as in lte wireless networks, to provide inter-cell coordination and interference mitigation are disclosed. A macrocell user equipment (ue) may determine information regarding an interfering femtocell node, such as a home enodeb (henb).
|Cell contacting arrangement for an energy storage device|
The present invention relates to a cell contacting arrangement for an energy storage module having a plurality of electrochemical storage cells, each storage cell having at least two electric connection terminals, the arrangement comprising: a carrier plate which is mountable on the energy storage module, and at least one cell connector inserted into the carrier plate for connecting at least two of the connection terminals of different storage cells, the carrier plate comprising at least one catch spring having a detent for fixing the cell connector in the carrier plate.. .
|Sodium/molybdenum composite metal powders, products thereof, and methods for producing photovoltaic cells|
A method for producing a metal article may include: producing a supply of a composite metal powder by: providing a supply of molybdenum metal powder; providing a supply of a sodium compound; combining the molybdenum metal powder and the sodium compound with a liquid to form a slurry; feeding the slurry into a stream of hot gas; and recovering the composite metal powder; and consolidating the composite metal powder to form the metal article, the metal article comprising a sodium/molybdenum metal matrix. Also disclosed is a metal article produced accordance with this method..
|Ohmic n-contact formed at low temperature in inverted metamorphic multijunction solar cells|
A method of forming a multijunction solar cell including an upper subcell, a middle subcell, and a lower subcell by providing a substrate for the epitaxial growth of semiconductor material; forming a first solar subcell on the substrate having a first band gap; forming a second solar subcell over the first solar subcell having a second band gap smaller than the first band gap; forming a graded interlayer over the second subcell, the graded interlayer having a third band gap greater than the second band gap; forming a third solar subcell over the graded interlayer having a fourth band gap smaller than the second band gap such that the third subcell is lattice mismatched with respect to the second subcell; and forming a contact composed of a sequence of layers over the first subcell at a temperature of 280° c. Or less and having a contact resistance of less than 5×10−4 ohms-cm2..
|Variable doping of solar cells|
A system and method for determining the edge or region where a saw first enters a silicon brick, and using this information to process this region differently is disclosed. This region, referred to as the saw entry region, may be thinner, or have a rougher texture than the rest of the substrate.
|Laboratory automated system with common sample buffer module|
A new laboratory automated system comprising a plurality of work cells coupled to a conveyor and a method for processing sample tubes are disclosed, both of which enable a system to maintain the maximum overall throughput regardless of the number and throughput of the individual work cells and regardless of the frequency at which sample tubes are loaded into the system. This is achieved by a sample buffer module coupled to the conveyor, the sample buffer module being in common to the plurality of work cells, and by a sample workflow manager configured to dispatch sample tubes from the sample buffer module to the work cells via the conveyor with a frequency for each work cell, which is equal to the sample processing throughput of each respective work cell..
|Method for the generation and cultivation of a plant cell pack|
The present invention relates to the generation and cultivation of plant cell material in the form of a non-tissue multilayer cell pack and its use for the accumulation or harvesting of a desired product. In particular, the invention provides a method for the generation of plant cell material in the form of a medium-deprived, porous structured and non-tissue multilayer cell pack and for the subsequent maintenance of said cell pack, comprising the steps of (i) providing a cell pack by separating cells from a plant cell suspension culture, wherein the content of the liquid comprised by the cell pack is reduced and adjusted to correspond to a cell pack density between 0.1 and 0.9 g wet cell weight per cm3, thereby establishing the medium-deprived and porous structured nature of said cell pack, and (ii) incubating said medium-deprived and porous structured cell pack in a non-liquid environment under a relative humidity of 50 to 100%..
|Islet cells from human embryonic stem cells|
This disclosure provides a system for producing pancreatic islet cells from embryonic stem cells. Differentiation is initiated towards endoderm cells, and focused using reagents that promote emergence of islet precursors and mature insulin-secreting cells.
|Method and device for producing and/or purifying polynucleotides and products obtainable thereof|
An apparatus and a method for obtaining a (poly)nucleotide sequence of interest include steps of cultivating hosts cells to produce a nucleotide sequence of interest and harvesting these cells, introducing these cells in a passageway and disintegrating them in a continuous process. In the continuous process, performing in the passageway a precipitation of contaminants by a mixing of the disintegrated cells with a solution containing one or more salt(s) and obtaining a mixture and allowing a precipitate to separate from the solution of this mixture, preferably to float and/or to sediment from the solution of this mixture for 1-48 hours and pumping out a soluble material from this solution, while excluding recovering the precipitate..
|Cassette for sterility testing|
The invention provides a device for growing cells—referred to as a cassette. The cell culturing device includes a housing that contains a lid having an optically clear window; a fluid distribution channel; a sample injection port fluidically connected to the fluid distribution channel; a base housing a porous media pad; and a media injection port fluidically connected to the media pad.
|Subtilase variants and polynucleotides encoding same|
The present invention relates to subtilase variants and methods for obtaining subtilase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants..
|Fungal gene expression using insulator dna sequences|
Described are compositions and methods relating to the expression of genes of interest in fungal cells using insulator dna sequences.. .
|Genetic regulation of bone and cells by electromagnetic stimulation fields and uses thereof|
The present invention provides methods to modify the genetic regulation of mammalian tissue, bone, cells or any combination thereof by preferential activation, up-regulation and/or down-regulation. The method comprises steps of tuning the predetermined profiles of one or more time-varying stimulation fields by manipulating the b-field magnitude, rising slew rate, rise time, falling slew rate, fall time, frequency, wavelength, and duty cycle, and exposing mammalian cells or tissues to one or more tuned time-varying stimulation fields with predetermined profiles.
|Glucoamylase variants and polynucleotides encoding same|
The present invention relates to glucoamylase variants having reduced sensitivity to protease nicking. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants..
|Xylitol production from cellulosic biomass|
The present disclosure relates to host cells containing a recombinant xylose reductase, a recombinant cellodextrin transporter, a recombinant intracellular β-glucosidase, and lacking xylitol dehydrogenase and xylulokinase, and to methods of using such cells for producing xylitol from cellulosic biomass containing cellodextrin and xylose.. .
|Process for preparing dicarboxylic acids employing fungal cells|
The present invention relates to a process for producing a dicarboxylic acid comprising fermenting a fungal cell in a vessel comprising a suitable fermentation medium, comprising adding a gas flow which comprises 20 to 35 v/v % of oxygen and less than 0.1v/v % of carbon dioxide to the fermentation medium, and maintaining an average partial carbon dioxide pressure of at least about 0.35 bar in the fermentation medium, and producing the dicarboxylic acid.. .
|Biocatalysts and methods for the synthesis of substituted lactams|
The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.. .
|Polypeptides having cellobiohydrolase activity and polynucleotides encoding same|
The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides..
|Dna polymerases with increased 3'-mismatch discrimination|
Disclosed are mutant dna polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods.
|Polypeptides having xylanase activity and polynucleotides encoding same|
Provided are isolated polypeptides having xylanase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains..
|Neutralizing gp41 antibodies and their use|
Monoclonal neutralizing antibodies are disclosed that specifically bind to the hiv-1 gp41 membrane-proximal external region (mper). Also disclosed are compositions including the disclosed antibodies that specifically bind gp41, nucleic acids encoding these antibodies, expression vectors including the nucleic acids, and isolated host cells that express the nucleic acids.
|Flow chamber analyte detection method|
A flow chamber and method for detecting the presence of one more cell produced analytes under flow conditions. The flow chamber includes two compartments separated by a permeable membrane on which a plurality of cells may be positioned.
|Disk-type microfluidic system and method for measuring deformability of blood cells|
A method for measuring a degree of deformation of blood cells includes: supplying blood to a centrifugal container of a disk; centrifuging the blood in the centrifugal container to blood cells and plasma by rotating the disk and detecting an actual moving distance of the blood cells in the centrifugal container every hour; and calculating a first curve representing the actual moving distance of the blood cells in the centrifugal container every hour and a second curve representing a theoretical moving distance of the blood cells every hour and measuring a degree of deformation of the blood cells by comparing the first curve and the second curve.. .
|Method and composition for a protein transduction technology and its applications|
A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier.
|Method for measuring cells, and reagent for cell measurement|
A method and a means are provided, by which multiple types of cells can be simultaneously measured with high sensitivity by an atp luminescence method. A method for measuring cells in a sample is provided, which comprises the steps of adding methanol to a sample suspected of containing viable cells to increase atp within viable cells, extracting intracellular atp, and causing extracted atp to emit luminescence..
|Acute leukemia and lymphoblastic lymphoma-specific cd43 epitope and use thereof|
The present invention relates to a cd43 epitope expressed on human acute leukemia and lymphoblastic lymphoma cells and its use. More particularly, the present invention relates to a cd43 epitope expressed on human acute leukemia, lymphoblastic lymphoma cells, but not on mature hematopoietic cells, hematopoietic stem cells and non-hematopoietic cells, and to its diagnostic and therapeutic application on acute leukemia and lymphoblastic lymphoma..
|Fluorescent two-hybrid (f2h) assay for direct visualization of protein interactions in living cells|
The present invention relates to an in vitro method for detecting protein-protein interactions comprising: (a) expressing in a eukaryotic cell a first fusion protein comprising (i) a (poly)peptide that, when expressed in a cell, accumulates at distinct sites in the nucleus of the cell or interacts with proteinaceous or non-proteinaceous structures accumulated at distinct sites in the nucleus of the cell; and (ii) a (poly)peptide specifically binding to gfp; (b) expressing in the same cell a second fusion protein comprising (i) gfp; and (ii) a bait (poly)peptide; (c) expressing in the same cell a third fusion protein comprising (i) a fluorescent (poly)peptide, the excitation and/or emission wavelength of which differs from that of gfp; and (ii) a prey (poly)peptide; and (d) detecting the fluorescence emission of the fluorescent parts of the second and the third fusion protein in the cell upon excitation, wherein a co-localization of the fluorescence emission of both fusion proteins in the cell nucleus is indicative of an interaction of the bait and the prey (poly)peptide. The invention also relates to an in vitro method for detecting protein-protein interactions comprising: (a) expressing in a eukaryotic cell a first fusion protein comprising (i) a fluorescent (poly)peptide; (ii) a (poly)peptide that, when expressed in a cell, accumulates at distinct sites in the nucleus of the cell; and (iii) a bait (poly)peptide (b) expressing in the same cell a second fusion protein comprising (i) a fluorescent (poly)peptide, the excitation and/or emission wavelength of which differs from that of the fluorescent (poly)peptide comprised in said first fusion protein; and (ii) a prey (poly)peptide and (c) detecting the fluorescence emission of the fluorescent parts of the first and the second fusion protein in the cell upon excitation, wherein a co-localization of the fluorescence emission of both fusion proteins in the cell nucleus is indicative of an interaction of the bait and the prey (poly)peptide.
|Transgenic non-human animal model for accelerated aging and/or age-related symptom, and use thereof|
A transgenic, non-human animal model for accelerated aging and/or age-related symptom, recombinant nucleic acid molecules, cells and methods that can be used to make such animal model and cells, methods of using the animal model and cells, to descendants of the transgenic non-human animal, obtained by breeding with the same or with another phenotype, and to a cell line or primary cell culture or to an organotypic brain slice culture, derived from the transgenic non-human animal or its descendants are disclosed.. .
The present invention relates to the generation of stable haploid cell cultures, uses of said cells in forward and reverse genetics, especially the identification of target genes associated with a modified phenotype and in particular identifying genetic targets associated with toxin resistance, especially ricin toxicity resistance, and therapeutic uses of target compounds.. .
|Compositions and methods for the storage of red blood cells|
The present invention provides an aqueous composition for storage of red blood cells consisting essentially of: adenine; dextrose; at least one nonmetabolizable membrane-protectant sugar; and a specifically defined ph buffering system. Also provided are improved methods for preserving red blood cells and methods for increasing the viability, membrane retention, and recoverability while suppressing apoptosis, hemolysis, and post-reinfusion clearance of stored red blood cells which utilize the novel compositions..
|Method of cryopreservation of tissue derived from pluripotent stem cells|
The invention provides a method of cryopreserving a tissue derived from a pluripotent stem cell, including the steps of (1) bringing a tissue derived from a pluripotent stem cell into contact with a cell protection solution that contains sulfoxide and chain polyol, (2) maintaining, in a cryopreservation solution, the pluripotent stem cell-derived tissue that was brought into contact with the cell protection solution in the first step, and (3) cryopreserving, in the presence of a coolant, the pluripotent stem cell-derived tissue that was maintained in the cryopreservation solution.. .
|Micromold methods for fabricating perforated substrates and for preparing solid polymer electrolyte composite membranes|
In polymer electrolyte membrane (pem) fuel cells and electrolyzes, attaining and maintaining high membrane conductivity and durability is crucial for performance and efficiency. The use of low equivalent weight (ew) perfluorinated ionomers is one of the few options available to improve membrane conductivity.
|Novel cu-based cermet materials for solid oxide fuel cells|
The present invention relates to a cermet body composition for the preparation of novel cermet materials to be used in solid oxide fuel cells. The cermet body composition comprises a ceramic component and a metallic component, wherein the ceramic component is in the range of 5% to 95% by wt of the cermet body..
|Fuel cell systems|
Described herein are fuel cell systems that include fuel cell covers, as well as electronic systems and methods for optimizing the performance of a fuel cell system. In the various embodiments, a fuel cell cover includes an interface structure proximate to one or more fuel cells.
|Integrated power generation and carbon capture using fuel cells|
Systems and methods are provided for capturing co2 from a combustion source using molten carbonate fuel cells (mcfcs). The fuel cells are operated to have a reduced anode fuel utilization.
|Air cell and assembled battery using the same|
An air cell includes a positive electrode layer, an electrolyte layer stacked on the positive electrode layer, a negative electrode layer stacked on the electrolyte layer and an electroconductive liquid-tight ventilation layer stacked on the positive electrode layer, the electroconductive liquid-tight ventilation layer being positioned on the opposite side of the positive electrode from the electrolyte layer. The assembled battery is provided with a plurality of the air cells described above.
A gravoltaic cell converts a gravitational force into electrical energy. The cell includes a reaction vessel and a first stationary homogeneous phase of dissociated aqueous cations and a second stationary homogeneous aqueous phase of dissociated aqueous reactant cations, both phases being disposed within the reaction vessel, and providing bulk solvent and anions a stationary bulk volume of a homogeneous mixture of solvent and dissociated anions collectively disposed homogeneously throughout the two layers of dissociated aqueous cations.
|Separator having a polysulfide barrier layer for lithium-sulfur cells|
A lithium-sulfur cell includes a lithium-containing anode, a sulfur-containing cathode and a separator arranged between the lithium-containing anode and the sulfur-containing cathode. To suppress a shuttle mechanism and to prevent a loss of active material, the separator includes a base layer and a polysulfide barrier layer.
|Battery pack of novel air cooling structure|
Disclosed herein is a battery pack including a plurality of battery modules, each having a battery cell or a unit module (unit cell) that can be charged and discharged, mounted in a pack case, wherein two or more unit cells constitute one battery module, two or more battery modules are arranged in a length direction of the battery pack to constitute one battery module group, two battery module groups are arranged in a width direction of the battery pack in a state in which the batter module groups are spaced apart from each other such that a coolant discharge part is defined between the battery module groups, a coolant inlet port is independently formed at a region of the pack case corresponding to each of the battery modules located at a position opposite to the coolant discharge part, and a coolant outlet port is formed at a front or a rear of the pack case in the length direction of the battery pack such that coolant introduced through the respective coolant inlet ports cools the unit cells of the respective battery modules while passing through the respective battery modules and is then discharged out of the pack case.. .
|Protective battery cell plates|
Apparatus for protecting battery cells. In some embodiments, a battery may comprise a series of battery cells and one or more protective plates positioned between the adjacent battery cells.
|Thermal management of electrical storage devices by coolant pool|
A battery system including a passive cooling system for cooling a plurality battery cells during normal operation and during overheating of the battery cells. The passive cooling system may include a housing, a cooling fluid (coolant pool) within the housing, and a plurality of dividers between each of the plurality of battery cells..
|Fluid-cooled battery module containing battery cells|
A battery module for receiving battery cells provides cooling through a cooling fluid. Chilled fluid travels first to the hottest part of the battery module and then continues to gradually less hot areas.
A battery pack includes a plurality of battery cells; a case accommodating the battery cells; a protective circuit module comprising a circuit board adjacent to the battery cells; a temperature sensitive element on the protective circuit module; and a flexible printed circuit board comprising a conductive pattern layer, wherein the conductive pattern layer is thermally connected to the temperature sensitive element.. .
|Rechargeable storage battery with improved performance|
A battery pack is provided for use with a wide variety of electronic and electrical equipment. Included is a battery pack for a cordless power device.
|Massaging floor mat with gel|
A massaging floor mat includes a layer of gel that penetrates into the cells of a layer of memory foam. The layer of foam sits on a foundation layer of hard plastic.
|Use of foti to enhance stem cell mobilization and proliferation|
The present invention provides a method of using polygonum multiflorum or extracts thereof to enhance stem cell mobilization in a subject, including hematopoietic stem cells (hscs) and bone marrow stem cells (bm-scs). In one embodiment, a blended composition of plant parts, fruits, mushrooms, microorganisms, maternal fluids, and extracts thereof are used to promote mobilization of stem cells, resulting in migration of the stem cells to specific sties of maintenance and repair within tissues and/or organs.