|| List of recent Amplification-related patents
|Simd instructions for data compression and decompression|
An execution unit configured for compression and decompression of numerical data utilizing single instruction, multiple data (simd) instructions is described. The numerical data includes integer and floating-point samples.
|Data compression and decompression using simd instructions|
Compression and decompression of numerical data utilizing single instruction, multiple data (simd) instructions is described. The numerical data includes integer and floating-point samples.
|Process for determining the concentration of nucleic acids|
A process is disclosed for determining the concentration of nucleic acids in a sample in a microfluidic device. In at least one embodiment, the method includes a) introducing the sample into a first chamber, b) carrying out a number of cycles of an amplification reaction to be carried out in cycles for amplifying nucleic acids, c) transferring a defined volume which is a fraction of the volume of the first chamber and which has amplified nucleic acids into a second chamber and replacing the transferred defined volume with fresh reagents for the amplification reaction, d) determining the concentration of the amplified nucleic acids in a second chamber equipped with an element to determine concentrations, and e) repeating steps b)-d) until a concentration of the amplified nucleic acids which is within a range is determined in the second chamber.
|Fabrication and use of a microfluidics multitemperature flexible reaction device|
Fabrication of a microfluidic multi-temperature reaction device (mmr) and the design and fabrication of the equipment to drive various molecular biological methods on the device are provided. The device can be applicable, for example, to nucleic acid (dna, rna, cdna, etc) amplification, cell lysis, reverse transcription and other enzymatic temperature sensitive and also temperature cycling reactions..
|Novel reagents for directed biomarker signal amplification|
Described herein are methods, compositions and articles of manufacture involving neutral conjugated polymers including methods for synthesis of neutral conjugated water-soluble polymers with linkers along the polymer main chain structure and terminal end capping units. Such polymers may serve in the fabrication of novel optoelectronic devices and in the development of highly efficient biosensors.
|Avalanche photodiodes and methods of fabricating the same|
Provided are an avalanche photodiode and a method of fabricating the same. The method of fabricating the avalanche photodiode includes sequentially forming a compound semiconductor absorption layer, a compound semiconductor grading layer, a charge sheet layer, a compound semiconductor amplification layer, a selective wet etch layer, and a p-type conductive layer on an n-type substrate through a metal organic chemical vapor deposition process..
|Microfluidic system for nucleic acid analysis|
A microfluidic system for analyzing nucleic acid, the microfluidic system including a reagent supply device including a sample chamber into which a sample can be injected, one or more reagent chambers for containing one or more reagents for extracting nucleic acid from the sample, and a waste chamber in which the used reagent can be discarded; a binding-lysis chamber in which cells are captured from the sample and lysed to form a cell lysate containing nucleic acid; plurality of particles for cell binding disposed in the binding-lysis chamber; a plurality of rehydration chambers into which the cell lysate formed in the binding-lysis chamber can be distributed and mixed with a nucleic acid amplification reagent to form an amplification reaction mixture; a plurality of amplification chambers in which a nucleic acid amplification reaction is performed on the amplification reaction mixture introduced from the plurality of rehydration chambers; and a flow channel system including an outlet and a plurality of inlets connected to the reagent supply device and forming an integrated fluid flow between the binding-lysis chamber, the rehydration chambers, and the amplification chambers.. .
|Method and kit for dna typing of hla gene|
The purpose of the present invention is to provide a method and kit for highly precise dna typing, in which ambiguity derived from phase ambiguity is eliminated. The present invention provides a method for the dna typing of hla, which is characterized by comprising: (1) a step of preparing a set of primers which can respectively anneal specifically to an upstream region and a downstream region of each of hla-a, hla-b, hla-c, hla-dqa1, hla-dqb1, hla-dpa1 and hla-dpb1 gene in the nucleotide sequence for the human genome, and a set of primers which can respectively anneal specifically to exon-2 and a 3′-side non-translated region in hla-drb1; (2) a step of carrying out the pcr amplification of a sample to be tested (dna) using the sets of primers; (3) a step of determining the nucleotide sequence for a pcr-amplified product; and (4) an optional step of carrying out the homology search in a data base..
|Methods and compositions for enrichment of nucleic acids in mixtures of highly homologous sequences|
Provided are methods of enrichment and detection of target nucleic acids during target amplification in the presence of excess amounts of highly homologous sequences, said methods having substantial diagnostic utility (e.g., cancer diagnostics). Provided are amplification reaction mixtures having at least one cleavage-directing oligonucleotide, the respective binding sites of which, for the target and homologous sequences, include one or more nucleotide positions differing in sequence between the target homologous sequences.
|Sequence amplification with linear primers|
The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences..
|Compositions for detecting small rnas|
Compositions and reaction mixtures are provided for the detection of small rna target nucleic acids, preferably mirna target nucleic acids, wherein the compositions and reaction mixtures provide for sensitive and specific detection of the target nucleic acids. The compositions and reaction mixtures include one or more of a first amplification oligomer that is preferably an extender primer, a target capture oligomer that is preferably at least partially double stranded, a promoter primer/provider, a reverse primer that is preferably a universal primer and a detection probe.
|Fluidic connectors and microfluidic systems|
Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels.
|Pattern forming method, chemical amplification resist composition and resist film|
A pattern forming method includes: (i) forming a film from a chemical amplification resist composition that contains (a) a resin, (b) a compound capable of generating an acid upon irradiation with an actinic ray or radiation and (c) a tertiary alcohol; (ii) exposing the film; and (iii) performing development by using a developer containing an organic solvent.. .
|Processing source video for real-time enhancement of a signal of interest|
What is disclosed is a system and method for real-time enhancement of an identified time-series signal of interest in a video that has a similar spatial and temporal structure to a given reference signal, as determined by a measure of closeness. A closeness measure is computed for pixels of each image frame of each channel of a multi-channel video to identify a time-series signal of interest.
|Sound processing apparatus, method, and program|
When equalizer processing for adjusting the gain of each frequency band of an input signal on the basis of a gain setting value is performed, an input signal is attenuated by an input attenuation amount derived from the gain setting value, and the equalizer processing is performed on the input signal attenuated. The amount of amplification of the gain of the input signal in the equalizer processing is estimated on the basis of the gain setting value and a weight coefficient of each frequency band derived from a generally-available music signal prepared in advance, and a difference of the estimation value and the input attenuation amount is calculated as a gain correction amount.
|Instrument amplification systems incorporating reflection cancelling boundary microphones and multiband compression|
Instrument amplification systems incorporating reflection cancelling boundary microphones and multiband compression in accordance with embodiments of the invention are disclosed. In one embodiment, an amplification system includes a microphone configured to generate an audio signal based upon acoustic waves within the instrument including audio content within a low frequency band including a fundamental resonant frequency of the instrument, a middle frequency band including at least one harmonic frequency of the instrument, and an upper frequency band, where the frequency response characteristics of acoustic waves generated within the instrument in the low frequency band differ from the characteristics of acoustic waves generated outside of the instrument, and an electronic preamplifier system configured to provide the audio content within the low frequency band to a low frequency band amplification circuit that applies compression to provide audio content having frequency characteristics corresponding to the characteristics of acoustic waves generated outside of the instrument..
|Solid-state imaging device and electronic apparatus|
A solid-state imaging device includes a pixel region in which shared pixels which share pixel transistors in a plurality of photoelectric conversion portions are two-dimensionally arranged. The shared pixel transistors are divisionally arranged in a column direction of the shared pixels, the pixel transistors shared between neighboring shared pixels are arranged so as to be horizontally reversed or/and vertically crossed, and connection wirings connected to a floating diffusion portion, a source of a reset transistor and a gate of an amplification transistor in the shared pixels are arranged along the column direction..
|Information processing device, information processing method, and imaging device|
An amplification detection unit detects an amplification of a motion of an image in a motion correction with respect to the motion of the image generated by the motion, according to motion information which indicates a motion detection result and a frame rate of the image. An information suppressing unit suppresses the motion indicated by the motion information based on the detection result of the amplification detection unit such that the motion of the image is not amplified in the motion correction, and generates corrected motion information used in the motion correction..
|Passive amplification circuit and analog-digital convertor|
A differential signal is amplified by passive amplification which does not a reference of a common-mode voltage. At this time, the voltage of the differential signal is passive-amplified twice before carrying out a successive approximation type analog-digital conversion operation.
|High-gain low-noise preamplifier and associated amplification and common-mode control method|
A preamplifier includes a differential pair of transistors receiving a bias current having a differential input and a differential output, a first resistor coupled to a first differential output node, a first transistor having a current path coupled between the first resistor and a power supply, a second resistor coupled to the first differential output node, a second transistor having a current path coupled between the second resistor and the power supply, a third resistor coupled to a second differential output node, a third transistor having a current path coupled between the third resistor and the power supply, a fourth resistor coupled to the second differential output node, and a fourth transistor having a current path coupled between the fourth resistor and the power supply, wherein a source of the second and third transistors are coupled together.. .
|Functionally integrated device for multiplex genetic identification|
A biochip for multiplex genetic identification is disclosed. An biochip for separating and detecting a plurality of dna fragments includes a set of inputs and chambers for receiving a sample matrix of genetic material and reagents needed to conduct a polymerase chain reaction amplification of the genetic material.
|Assay and other reactions involving droplets|
The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel.
|Assays and other reactions involving droplets|
The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel.
|Compositions and methods for rt-pcr|
The present invention relates to methods and compositions having trehalose and dna polymerase for facilitating the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of rna molecules, and for increasing the detection sensitivity and reliability through generation of secure cdna molecules prior to gene-specific primer dependent amplification. The reagent mixture comprises a ready to use reagent solution, wherein the solution comprises: (a) trehalose in a concentration between about 5% and about 35%; (b) a viral reverse transcriptase; and (c) at least one dna polymerases, in a buffer suitable for use in a reverse transcription reaction, wherein the buffer comprises a co-factor metal ion and nucleoside triphosphates..
|Nucleic acid sequences that can be used as primers and probes in the amplification and detection of all subtypes of hiv-1|
The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with the aids causing human immuno-deficiency virus (hiv). With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of hiv-1 nucleic acid.
|Radio frequency signal transceiving and processing method, device, and base station system|
A radio frequency signal transceiver includes: a transmission circuit configured to perform power amplification on an input first analog signal, wirelessly transmit the first analog signal after power amplification, and output the first analog signal after power amplification to a pre-distortion circuit; the pre-distortion circuit configured to convert the first analog signal after power amplification into a second analog signal and output the second analog signal, where the second analog signal is used to feedback distortion of the first analog signal to compensate the first analog signal in advance according to the distortion; and a receiving circuit configured to wirelessly receive a third analog signal, and process and output the third analog signal. The radio frequency signal transceiver can improve efficiency in receiving and transmitting a radio frequency signal, reduce a cost of a base station system, and reduce a difficulty in implementing the base station system..
|Optical phase-sensitive amplifier for dual-polarization modulation formats|
A method for amplifying optical signals includes determining a source optical signal, generating a first resultant signal including a pump signal and the source optical signal, sending the first resultant signal through a non-linear element to generate a second resultant signal including the first resultant signal and an idler signal, and sending the second resultant signal through a non-linear element to perform phase-sensitive amplification. The phase-sensitive amplification results in a third resultant signal including an amplified source optical signal, the pump signal, and the idler signal.
|Solid-state imaging element having image signal overflow path|
Since the great number of elements constituting a unit pixel having an amplification function would hinder reduction of pixel size, unit pixel n,m arranged in a matrix form is comprised of a photodiode, a transfer switch for transferring charges stored in the photodiode, a floating diffusion for storing charges transferred by the transfer switch, a reset switch for resetting the floating diffusion, and an amplifying transistor for outputting a signal in accordance with the potential of the floating diffusion to a vertical signal line, and by affording vertical selection pulse φvn to the drain of the reset switch to control a reset potential thereof, pixels are selected in units of rows.. .
|Amplification circuit having optimization of power|
By activating or deactivating one or more of the n cascode circuits, the total size of the amplification components can be adapted to the value of the output power to generate.. .
|Noninvasive prenatal molecular karyotyping from maternal plasma|
Disclosed herein are methods, systems, and apparatus for detecting microamplifications or microdeletions in the genome of a fetus. In some embodiments, the method comprises receiving sequence tags for each of a plurality of dna fragments in a biological sample; determining genomic positions for the sequence tags; determining whether the density of dna in each of a plurality of genomic regions is aberrantly high or low; identifying as a microamplification a set of consecutive genomic regions having aberrantly high density; and identifying as a microdeletion a set of consecutive genomic regions having aberrantly low density.
|Method for preparing nucleic acid aptamer|
An object of the present invention is to develop and provide a method for efficiently and conveniently producing a nucleic acid aptamer, particularly, a dna aptamer, having high specificity for and high binding activity against a target substance. The present invention provides a method for producing a nucleic acid aptamer, comprising: a complex formation step of mixing a single-stranded nucleic acid library with a target substance in a solution to form a complex of a single-stranded nucleic acid and the target substance; an immobilization step of mixing the solution after the preceding step with a solid-phase support to immobilize the complex onto the solid-phase support via connector(s) adsorbed on the target substance and/or the solid-phase support; a recovery step of recovering the complex immobilized on the solid-phase support from the solution; an amplification step of recovering the single-stranded nucleic acid from the complex, followed by amplification by a nucleic acid amplification method; and a single-stranded nucleic acid preparation step of converting the double-stranded nucleic acids obtained in the amplification step into single strands and then forming an intramolecular conformation..
|Genetic test system|
There is provided a genetic test system provided with a dispensing means for dispensing a sample and a reagent to a reaction vessel and a vessel conveyance means for conveying the reaction vessel. The system further includes a plurality of nucleic acid amplification detection units, each including a temperature control means for accommodating a plurality of reaction vessels and performing temperature control for each accommodated position of the reaction vessel, a temperature monitoring means for monitoring a value of temperature to be controlled of the reaction vessel, and a light emission measurement means for measuring light emission of reaction liquid in the reaction vessel.
|Methods and compositions for modulation of amplification efficiency|
Provided herein are methods and kits for modulating the amplification efficiency of nucleic acids, which are useful in multiplex reactions where the amplification efficiency of one or more nucleic acids in the mixture are desired to be modulated relative to one or more other nucleic acids. Embodiments relate to molecular diagnostics, including detecting sequence variants, such as snps, insertions deletions, and altered methylation patterns, as well as the modulation of the amplification efficiency of internal control sequences to provide more accurate control sequences for amplification reactions..
|Single-cell nucleic acid analysis|
The present invention provides methods for analysis of genomic dna and/or rna from small samples or even single cells. Methods for analyzing genomic dna can entail whole genome amplification (wga), followed by preamplification and amplification of selected target nucleic acids.
|Amplification method for photoresist exposure in semiconductor chip manufacturing|
An electrical field is applied through an extreme ultraviolet (euv) photoresist layer along a direction perpendicular to an interface between the euv photoresist layer and an underlying layer. Secondary electrons and thermal electrons are accelerated along the direction of the electrical field, and travel with directionality before interacting with the photoresist material for a chemical reaction.
|Optical receiving circuit|
An optical receiving circuit includes: a first non-feedback amplifier configured to convert a current signal, obtained from a light receiving element in response to an optical signal, into a first voltage signal; a second amplifier configured to convert an input current signal into a second voltage signal, the output signal not being directly fed back to an input side; a differential amplifier configured to perform differential amplification on the first voltage signal and the second voltage signal and to output an in positive signal and a negative signal obtained through the differential amplification; and an offset compensation circuit configured to input, on the basis of the in positive signal and the negative signal output from the differential amplifier, an offset current signal in accordance with an offset of a level of the in positive signal from a level of the negative signal to the second amplifier.. .
|Hearing aid and method for eliminating acoustic feedback in the amplification of acoustic signals|
In a hearing aid having a microphone to be arranged at a body of a user for capturing ambient sound, a loudspeaker for outputting the ambient sound after having been amplified on a frequency-dependent basis, and a signal processor, the signal processor is configured to amplify the sound such that the amplified sound is audible to the user, and to automatically re-adjust the gain by the following steps: selecting a tone having a specific frequency; outputting the tone and the amplified ambient sound via the loudspeaker as an output sound; capturing via said microphone an analysis sound composed of ambient sound and of a reflection of said output sound; extracting a reflection of the tone from the captured analysis sound; and determining a reflection component for the specific frequency of the tone; adjusting the gain in frequency-specific manner and based on the determined reflection component.. .
|Method of and apparatus for reducing papr in filter-bank multi-carrier system|
The invention relates to a method of reducing peak-to-average. Power ratio in a transmitting device of a filter-hank multi-carrier system, which includes the steps of: performing constellation modulation (210) on data to be transmitted; performing k-point discrete fourier transform (220) on a vector composed of k constellation symbols resulting from the constellation modulation: and performing offset-quadrature amplitude modulation (230) on a data vector resulting from the discrete fourier transform, wherein the parameter k represents the number of subcarriers allocated for transmission of the data to be transmitted.
|Image pickup apparatus, image pickup system and driving method of image pickup apparatus|
An image pickup apparatus of an embodiment includes pixel units each including a photoelectric conversion unit and an amplification transistor that outputs a signal based on an electric carrier generated by the photoelectric conversion unit, a first output line to which signals from first and other pixel units are output, and a second output line to which signals from second and other pixel units are output. A connection unit is arranged to control an electric connection between input nodes of the amplification transistors of the first and second pixel units is arranged.
|N-way doherty distributed power amplifier with power tracking|
A power amplifier using n-way doherty structure with adaptive bias supply power tracking for extending the efficiency region over the high peak-to-average power:ratio of the multiplexing modulated signals such as wideband code division multiple access and orthogonal frequency division multiplexing is disclosed. In an embodiment, present invention uses a dual-feed distributed structure to an n-way doherty amplifier to improve the isolation between at least one main amplifier and at least one peaking amplifier and, and also to improve both gain and efficiency performance at high output back-off power.
|Low drop-out regulator|
Exemplary embodiments disclose a low drop-out regulator including an error amplification unit which includes a zero compensation circuit configured to compensate a plurality of poles which are generated by an output terminal and a buffer, the error amplification unit is configured to generate a first comparison signal in response to a reference voltage and a feedback voltage, the buffer is configured to generate a second comparison signal in response to the first comparison signal and an input voltage, a pass unit configured to provide an output voltage and a load current to the output terminal in response to the second comparison signal and the input voltage, and a feedback unit configured to provide the feedback voltage to the error amplification unit in response to the output voltage. A driving current of the buffer is independently adjusted with respect to the load current..
|Amplification of cyp24 and uses thereof|
This invention pertains to the discovery that an amplification of the cyp24 gene or an increase in cyp24 activity is a marker for the presence of, progression of, or predisposition to, a cancer (e.g., breast cancer). Using this information, this invention provides methods of detecting a predisposition to cancer in an animal.
|Rf power transmission, modulation, and amplification embodiments|
Methods and systems for vector combining power amplification are disclosed herein. In one embodiment, a plurality of signals are individually amplified, then summed to form a desired time-varying complex envelope signal.
|Nano-pcr: methods and devices for nucleic acid amplification and detection|
Methods, devices, and compositions are described that provide for amplification of nucleic acid sequences without reliance upon temperature cycling, thus freeing the methods from conventional benchtop thermal cycling devices. Denaturation of double stranded nucleic acids, primer annealing, and precision control over primer extension by polymerase can be accomplished by applying stress to a nucleic acid.
|Linear amplification of short nucleic acids|
The present teachings provide novel methods for amplifying short nucleic acids. In some embodiments, the present teachings provide novel methods for linearly amplifying a collection of micro rnas by using temperature cycling during a reverse transcription reaction.
|Endonuclease-enhanced helicase-dependent amplification|
The invention provides methods and compositions for enhancing the speed and sensitivity of helicase-dependent amplification through the use of an endonuclease.. .
|Pyrophosphorolysis-activated polymerization (pap) using ribonucleic acid (rna) template|
A new method of rna-pap was developed that can directly amplify rna template without additional treatment. Rna-pap brings in a new mechanism for amplification of rna template in which rna-dependent dna pyrophosphorolysis and rna-dependent dna polymerization are serially coupled using 3′ blocked primers.
|Methods for determining cell viability using molecular nucleic acid-based techniques|
The present invention relates to novel methods, and kits, for selectively excluding dead cells from a mixture containing live and dead cells, such as microbe cells in clinical samples, blood products, medical/biotechnology products and food products where subsequent interrogation of the selected live cells are an indicator of the presence of microbe viability. In particular, the invention relates to improved methods for performing direct nucleic acid amplification techniques such as polymerase chain reaction (pcr) and isothermal techniques in blood and other body fluids, for correlation with microbe cell viability from bacteremia and fungemia samples.
|Assays for the detection of genotype, mutations, and/or aneuploidy|
The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal dna present in a maternal bodily fluid sample..
|Noninterfering multipurpose compositions for collecting, transporting and storing biological samples|
The invention is directed to compositions and methods for collecting, transporting, and storing microorganisms obtained from samples of biological, clinical, forensic, and environmental origin. Compositions preserve the viability of the collected organisms, permit long-term storage, and are compatible with subsequent manipulation including propagation and culture of collected microorganisms, or isolation, purification, detection, and characterization of proteins, nucleic acids and macromolecules.
|Alarm device for banishment of birds and animals|
This utility model releases an alarm device for banishment of birds and animals, which includes: radio transmission module, voltage stabilizing circuit and inverter circuit. The radio transmission module sends radio signals to radio receiver module which then transmits such radio signals to the pc control circuit; the said pc control circuit sends instruction signals to the audio circuit which then transmits the audio signal to amplification circuit which outputs the amplified audio signals; the said voltage stabilizing circuit provides 5v power supply to the said radio transmission module, radio receiver module, the said pc control circuit and the said audio circuit, while the said inverter circuit provides +/−35v power supply to the said amplification circuit.
|Spectroscopic analysis method and spectroscopic analyzer|
The present invention improves an s/n ratio and light intensity resolution provided by an a/d converter, and an analyzer includes: a measurement cell irradiated with light during sample measurement; a dimming element irradiated with the light during reference measurement; an amplifier configured to amplify an analog light intensity signal outputted from a light detector; an a/d converter configured to convert the analog light intensity signal into a digital light intensity signal; and an arithmetic device configured to calculate absorbance using a digital sample light intensity signal outputted from the a/d converter during the sample measurement and a digital reference light intensity signal outputted from the a/d converter during the reference measurement, wherein an amplification factor of the amplifier is set such that the analog reference light intensity signal and the analog sample light intensity signal become less than or equal to a full scale of the a/d converter.. .
|High efficiency output stage amplification for radio frequency (rf) transmitters|
Highly power efficient transmitter output stage designs are provided. In an embodiment, the probability density function (pdf) of an input signal is divided into a plurality of regions, and samples of the input signal are processed depending on the region of the pdf within which they fail.
|Ask modulation amplification circuit|
An amplitude shift keying (ask) modulation amplifier circuit includes a first amplifier to which a high frequency signal and a modulating signal are supplied, and that is configured to perform an amplification of the high frequency signal and an ask modulation, and a second amplifier to which an output of the first amplifier and the modulating signal are supplied, and that is configured to perform an amplification of the output signal from the first amplifier and an ask modulation. In some configurations, an amplification gain of the second amplifier is set higher than an amplification gain of the first amplifier..
Provided is a feedback amplifier. The feedback amplifier includes: an amplification circuit unit amplifying a bust packet signal inputted from an input terminal and outputting the amplified voltage to an output terminal; a feedback circuit unit disposed between the input terminal and the output terminal and controlling whether to apply a fixed resistance value to a signal outputted to the output terminal; a packet signal detection unit detecting a peak value of a bust packet signal from the output terminal and controlling whether to apply the fixed resistance value; and a bias circuit unit generating a bias voltage, wherein the feedback circuit unit determines a feedback resistance value to change the fixed resistance value in response to at least one control signal and adjusts a gain by receiving the bias voltage..
|Phase lock loop controlled current mode buck converter|
A current mode buck converter has a power stage and a feedback stage. The power stage converts a higher power supply voltage level to a lower output voltage level.
|Long-distance polarization and phase-sensitive optical time-domain reflectometry based on random laser amplification|
A long-distance polarization and phase-sensitive reflectometry based on random laser amplification for extending a sensing distance includes a long-distance polarization and phase-sensitive reflectometry of a distributed raman amplification based on optical fiber random lasers generated by unilateral pumps, a long-distance polarization and phase-sensitive reflectometry of a distributed raman amplification based on optical fiber random lasers generated by bilateral pumps, and a long-distance polarization and phase-sensitive reflectometry of a raman amplification based on a combination of optical fiber random lasers generated by unilateral pumps and a common raman pump source, which are applied in optical fiber perturbation sensing and have a capability of greatly improving a working distance of a sensing system and a high practicability.. .
|Yield traits for maize|
Methods for introgressing an allele of interest of a locus associated with a yield trait into zea mays germplasm are provided. In some embodiments, the methods include providing a zea mays plant that contains an allele of interest of a locus associated with a yield trait, wherein the locus associated with the yield trait is identifiable by pcr amplification of a zea mays nucleic acid with a pair of oligonucleotides primers as disclosed herein, and introgressing the allele of interest into zea mays germplasm that lacks the allele.
|Priority-based garbage collection for data storage systems|
Priority-based garbage collection utilizes attributes of data stored in the non-volatile memory array in order to improve efficiency of garbage collection and of the overall data storage system. A set of low priority data can be selectively evicted from a non-volatile memory array.
|Dynamic overprovisioning for data storage systems|
Disclosed embodiments are directed to systems and methods for dynamic overprovisioning for data storage systems. In one embodiment, a data storage system can reserve a portion of memory, such as non-volatile solid-state memory, for overprovisioning.
|Diagnosis, prognosis and identification of potential therapeutic targets of multiple myeloma based on gene expression profiling|
Gene expression profiling is a powerful tool that has varied utility. It enables classification of multiple myeloma into subtypes and identifying genes directly involved in disease pathogensis and clinical manifestation.
|Time alignment for an amplification stage|
A method of calibrating a polar amplification stage including a main signal path and a magnitude signal path, the method comprising: generating signals (108, 142) for the main signal path and the magnitude signal path for operating an amplifier (102) between a linear mode of operation and a saturated mode of operation; detecting (114) first and second peaks in a signal at the output of the amplifier (102) representing transitions between the linear and saturated modes of operation; and adjusting (124) the timing in one of the main signal path and the amplitude signal path in dependence on a relative difference between the size of the detected first and second peaks.. .
|Broadband re-configurable rf transmitter|
An rf transmitter system operating on a broader frequency range and providing a higher output power dynamic range is described. Low power rf signal is produced from a baseband signal using a quadrature modulator and using a broadband local oscillator signal.
|Methods and products for producing engineered mammalian cell lines with amplified transgenes|
Methods of inserting genes into defined locations in the chromosomal dna of cultured mammalian cell lines which are subject to gene amplification are disclosed. In particular, sequences of interest (e.g., genes encoding biotherapeutic proteins) are inserted proximal to selectable genes in amplifiable loci, and the transformed cells are subjected to selection to induce co-amplification of the selectable gene and the sequence of interest.
|Methods for nucleic acid manipulation|
A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor.
|Compositions and methods for the protection of nucleophilic groups|
The present invention provides compositions, methods, and kits relating to the protection and deprotection of molecules comprising nucleophilic groups, such as the protection and deprotection of thermostable polymerases. Also provided are methods of performing nucleic acid amplification using polymerases protected according to the invention..
|Method for dna amplification based on the origins of replication of the bacteriophage p29 and associated nucleotide sequences|
The present invention refers to a dna amplification method based on the origins of replication of bacteriophage φ29, and to the genic constructs, vectors and oligonucleotides that can be used in the method for amplifying an exogenous sequence of interest.. .
|Method for stable gene-amplification in a bacterial host cell|
A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.. .
|Digital assays with a generic reporter|
Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.. .
|Method for the identification of propane-oxidizing bacteria|
The invention relates to a method for the identification of propane-oxidizing bacteria which is based on the identification of at least one fragment of the prma gene encoding the alpha subunit of the propane monooxygenase enzyme and/or the prmd gene encoding an ancillary protein involved in the oxidation reaction of propane by gene amplification in the presence of pairs of primers selected in correspondence of homologous portions, deduced from the alignment of the prma and prmd sequences.. .
|Nucleic acid binding dyes and uses therefor|
The invention provides novel compounds and compositions of formulas i and ii, as well as methods of using them. The compounds can be used, for example, to quantify an amount of double stranded dna in a sample subjected to nucleic acid amplification, or for real time monitoring of a nucleic acid amplification reaction.
|Oligonucleotids comprising a label associated through a linker|
The present application discloses a labeled nucleotide comprising a label attached via a linker, wherein said labeled nucleotide has the formula wherein r1 is a residue with a negative net charge, preferably selected from the group consisting of a phosphate group, and a sulphate group; wherein r2, r3 and r4 are independently selected from the group consisting of h2, oh2, and o; wherein “n” is an integer between 0 and 16; wherein “a” is an integer between 1 and 10; wherein sp is absent or a spacer; wherein x is said label; and wherein y is a nucleotide or nucleoside. Furthermore, oligonucleotides comprising a labeled nucleotide according to the present invention and the use as a primer in amplification based methods is disclosed herein..
|Selective detection of neisseria meningitidis|
A process for detecting neisseria meningitidis nucleic acid in a sample is provided including producing an amplification product by amplifying neisseria meningitidis nucleotide sequence of the sodc gene or mrna using a forward primer of seq id no: 1, and a reverse primer of seq id no: 2, and detecting the amplification product to detect neisseria meningitidis in the sample. Also provided are reagents and methods for detecting and distinguishing neisseria meningitidis from other infectious agents.
|Bridge fiber, combiner, and fiber laser device|
A bridge fiber includes a core layer 31 and an outer layer 32 which has an index of refraction higher than that of the core layer 31 and covers the outer peripheral surface of the core layer 31. The outer layer 32 is surrounded by a substance such as the atmosphere having an index of refraction lower than an index of refraction n2 of the outer layer 32.