|| List of recent Amplification-related patents
| Photoelectric conversion apparatus and photoelectric conversion system|
A photoelectric conversion apparatus including a plurality of photoelectric conversion units each of which includes a first electrode, a second electrode, a photoelectric conversion layer which accumulates signal charges and which is disposed between the first and second electrodes, and an insulating layer disposed between the photoelectric conversion layer and the second electrode, an amplification unit configured to receive optical signals and output signals each based on one of the optical signals, each of the optical signals being based on one of the signal charges, each of the signal charges being accumulated in one of the plurality of photoelectric conversion units, and a capacitive element having a first node and a second node, the first node being connected to the second electrodes of the plurality of photoelectric conversion units and the amplification unit and the second node selectively receiving each one of a plurality of potentials having different values.. .
Canon Kabushiki Kaisha
| Solid-state image device, driving solid-state imaging device, and electronic system|
A solid-state imaging device includes: a pixel array section in which a plurality of pixels including an amplification transistor configured to amplify a signal based on a photoelectric charge in accordance with an amount of received light are disposed; through vertical signal lines of the pixel array section, a bias-current control section configured to turn on or off a bias current supplied to the amplification transistor for each of the vertical signal lines; and a drive control section configured to control the bias-current control section so as to turn on the bias current of the vertical signal line through which a pixel signal is read, and to turn off the bias current of the vertical signal line through which a pixel signal is not read.. .
| Photoelectric conversion apparatus, photoelectric conversion system, and driving the photoelectric conversion apparatus|
An output control unit configured to set an amplitude of a signal output to a signal line to be smaller as compared with a case where an amplification unit outputs a signal on the basis of a potential of the input node or put the amplification unit into a non-operating state is provided.. .
Canon Kabushiki Kaisha
| Improved dynamic compressor with "release" feature|
A dynamic range compressor includes an input terminal for receiving input signal to be compressed, an amplifier unit for amplifying the signal to be compressed by an amplification factor, for deriving a compressed output signal, an output terminal for supplying the compressed output signal, a first envelope detector unit for deriving a first envelope signal from the input signal, and an amplifier control unit for generating an amplifier control signal in dependence of an envelope signal. Further including a second envelope detector unit for deriving a second envelope signal from the input signal, a first signal level prediction unit for generating a first prediction signal from the first envelope signal, a second signal level prediction unit for generating a second prediction signal from the second envelope signal, and a signal combination unit for combining the first and second prediction signals to generate a combined prediction signal..
Institut FÜr Rundfunktechnik Gmbh
| Multiple-port signal boosters|
A signal booster is disclosed that includes a first interface port, a second interface port, a downlink signal splitter device, an uplink signal splitter device, a main booster and a first front-end booster. The downlink signal splitter device can communicate a downlink signal from the first interface port to a plurality of interface ports.
Wilson Electronics, Llc
| Apparatus and methods for overdrive protection of radio frequency amplifiers|
Provided herein are apparatus and methods for overdrive protection of radio frequency (rf) amplifiers. In certain configurations, an rf amplifier includes a plurality of amplification stages and an overdrive detection circuit.
Skyworks Solutions, Inc.
| Linearized gate capacitance in power amplifiers|
An apparatus includes: a plurality of amplification stages, each stage comprising a cascode transistor; and a bridge circuit coupled between gate terminals of cascode transistors in two adjacent stages of the plurality of amplification stages, the bridge circuit including a plurality of diodes.. .
| Electromagnetic energy device with improved coil and use|
An apparatus and method are shown for improving the efficiency of motors, generators and machines by using a closed ferromagnetic frame upon which is mounted a plurality of solenoid coils. Two of the solenoid coils are wired together so that they act as one in a closed wiring circuit.
| Signal strength enhancement in a biometric sensor array|
A biometric imager may comprise a plurality of sensor element traces formed in or on a sensor substrate which may comprise at least a portion of a display screen defining a biometric sensing area and forming in-active pixel locations; an auxiliary active circuit formed in or on the sensor substrate on the periphery of the biometric sensing area and in direct or indirect electrical contact with the sensor element traces; and providing a signal processing interface to a remotely located controller integrated circuit. The sensor element traces may form a portion of one dimensional linear sensor array or pixel locations in a two dimensional grid array capacitive gap biometric imaging sensor.
| Non-saturating receiver design and clamping structure for high power laser based rangefinding instruments|
A non-saturating receiver design and clamping structure for high power laser rangefinders of especial utility with respect to pumped, monoblock lasers. The receiver comprises a photodiode and a transimpedance amplifier having at least first and second stages.
Kama-tech (hk) Limited
Analyte detection enhancement by targeted immobilization, surface amplification, and pixelated reading and analysis
This disclosure provides, among other things, a method of sample analysis, that comprises: (a) binding target analytes to capture agents that are attached to a surface of a plate, wherein the plate comprises (i) a sensing amplification layer comprises nanostructures that enhance signals and (ii) the capture agents are attached to said sensing amplification layer; (b) reading the plate with a reading device to produce an image of signals that represent individual binding events; and (c) identifying and counting individual binding events in an area of the image, thereby providing an estimate of the amount of one or more analytes in the sample. A system for performing the method is also provided..
The Trustees Of The Princeton University
Assay enhancement by selective deposition and binding on amplification structures
This disclosure provides, among other things, a method for enhancing detection of an analyte that is bound to a substrate comprising a signal amplification layer on a surface of the substrate, wherein the signal amplification layer comprises high-amplification regions and low-amplification regions, and the high-amplification regions amplify signals at said surface more than the low-amplification regions. The method comprises selectively masking the low-amplification regions of the substrate, thereby increasing the probability that an analyte will bind to a high-amplification region and be detected..
The Trustees Of Princeton University
Core reactor and system
A core reactor comprises a multistage single, dual, multi-directional or reversible flow system including at least: 1) a power generation stage; 2) a power amplification stage or stages; 3) apparatus feed and/or an internal processing system; and an optional flow recycle and/or propulsion stage. The core reactor can include the following interconnected components: 1) primary kinetic energy device (s); exhaust nozzles; 2) single or multilevel swirl chambers; 3) single or multiple conical vortex cones; and 4) modified vortex tubes(s) for cryogenic, sonic or extreme thermal heart generation streams.
Transtar Group, Ltd
Compositions and methods for detecting hev nucleic acid
Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of hepatitis e virus (hev) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits..
Method for the prognosis and treatment of cancer metastasis
The present invention relates to a method for the prognosis of bone metastasis in cancer which comprises using a probe to determine if a gene of interest is amplified in a primary tumor sample. Likewise, the invention also relates to a method for determining the tendency to develop bone metastasis with respect to metastasis in other organs, which comprise using a probe to determine the expression level of a gene of interest, or the amplification or translocation of a gene of interest.
Institucio Catalana De Recerca I Estudis Avancats
Method for the prognosis and treatment of renal cell carcinoma metastasis
The present invention relates to a method for the prognosis of bone metastasis in renal cell carcinoma which comprises determining if the c-maf gene is amplified in a primary tumor sample. Likewise, the invention also relates to a method for determining the tendency to develop bone metastasis with respect to metastasis in other organs, which comprise determining the c-maf gene expression level, amplification or translocation.
Nucleic acid amplification
In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions.
Life Technologies Corporation
Recombinase polymerase amplification
This disclosure describes related novel methods for recombinase-polymerase amplification (rpa) of a target dna that exploit the properties of recombinase and related proteins, to invade double-stranded dna with single stranded homologous dna permitting sequence specific priming of dna polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes.
Alere San Diego, Inc.
Systems and methods for multiplex analysis of pcr in real time
The present invention provides methods and systems for real-time measurements of pcr with multiplexing capability. Certain embodiments relate to methods and systems that use fluorescently encoded superparamagnetic microspheres for the immobilization of amplification products during the pcr process, and an imaging chamber of a measurement device that is also capable of controllable thermal cycling for assisting the pcr process..
Novel compositions and processes for analyte detection, quantification and amplification
This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes.
Enzo Biochem, Inc.
A real-time method of detecting the presence and/or amount of a methylated or unmethylated gene of interest in a dna-containing sample, comprises the steps of (a) contacting the dna-containing sample with a reagent which selectively modifies unmethylated cytosine residues in the dna to produce detectable modified residues but which does not modify methylated cytosine residues (b) amplifying at least a portion of the methylated or unmethylated gene of interest using at least one primer pair, at least one primer of which is designed to bind only to the sequence of methylated or unmethylated dna following treatment with the reagent, wherein at least one primer in the primer pair produces a detectable fluorescence signal during amplification which is detected in real-time (c) quantifying the results of the real-time detection against a standard curve for the methylated or unmethylated gene of interest to produce an output of gene copy number.. .
Multiplex assay for detection of bacterial species in biological samples
The invention provides a rapid, accurate, sensitive, and low-cost detection method for screening a biological sample for one or more desired bacterial species. The inventive method employs a two-step multiplex real-time pcr assay that comprises an internal amplification control and specific primer sets to detect and discriminate bacterial species based the unique melting temperatures of specific dna sequences of each strain..
The Curators Of The University Of Missouri
Method for improved thermocycling of low volume nucleic acid amplification reactions
A processing module is configured to extend the capabilities of an analyzer configured to process substances within each of a plurality of receptacles. The module includes a container transport configured to transport a container from a location within the processing module to a location within the analyzer that is accessible to a substance transfer device of the analyzer.
Methods for the production of libraries for directed evolution
Disclosed herein is an efficient method of generating a library of variants of a sequence of interest, such as may be used in directed evolution, in one embodiment, the method includes an amplification reaction, e.g. Error-prone pcr, to generate double-stranded dna (dsdna) variants of a sequence of interest, after which one strand of the dsdna variants may be selectively degraded to produce single-stranded dna (ssdna) variants.
Buffers for use with polymerases
amplification reaction mixtures comprising a cationic surfactant and an anionic surfactant are provided.. .
Bio-rad Laboratories, Inc.
Methods and compositions for pcr
A modified thermostable pol b dna polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable pol b dna polymerase and a modifier reagent of formula i wherein the reaction results in a thermally reversible inactivation of the thermostable pol b dna polymerase activity and the 3′-5′ exonuclease activity, which polymerase is suitable for hot-start pcr. Also disclosed are the method for the modification, a polynucleic acid amplification method and pcr reaction mixture and kit comprising the modified thermostable pol b dna polymerase..
Life Technologies Corporation
Intraocular lens with electricity generator and additional functional systems
An accommodating intraocular lens combination with two optical elements which move versus each other. The optical elements have such a shape that the combination provides variable focusing power at different positions of at least one of the optical elements relative to the other optical element.
Akkolens International B.v.
Physiologic monitoring systems and methods
According to an aspect, an apparatus comprises an integrated circuit and a memory circuit to process input signals obtained from a user according to a plurality of physiologic monitoring uses, and a physiologic monitoring sensor circuit, including at least one physiologic sensor and physiologic signal amplification and filtering circuitry, applied to the user's skin by coupling physiologic signals from the user's skin, to receive physiologic signals received by the coupling, and to amplify and filter the received physiologic signals for processing by the integrated circuit and therefrom measure a plurality of physiologic parameters of the user according to the physiologic monitoring uses. The apparatus comprises an external removable circuit to enable additional physiological monitoring uses, and verification and activation circuitry to receive initialization data from the external removable circuit and configure the integrated circuit and the physiologic monitoring sensor circuit to provide at least one additional physiologic parameter..
Method for processing double click event for securing safety in quantum key distribution system
A method performed by a processor of a receiver of generating a secret key using a quantum communication, the processor execute the method comprising: performing a quantum key distribution (qkd) protocol to generate a sifted key; performing a post-processing protocol based on the sifted key to generate a final key; counting the number of double click events when quantum signals are detected; and performing privacy amplification of the final key based on the counted number of the double click events.. .
Sk Telecom Co., Ltd.
Communication device, reception power monitoring device, and communication device system
A communication device system includes a communication device (1) and a reception power monitoring device (2). The communication device (1) includes a reception processing unit (10) that outputs a signal intensity voltage (vin) in accordance with a signal level of a received signal, and a monitoring terminal signal generator circuit (30) that generates a monitoring terminal signal (vout) by current amplification of a level shift voltage generated by shifting a level of the signal intensity voltage (vin) by an offset voltage (voff) corresponding to a voltage value of a power supply voltage of an external device.
Multiple-port signal boosters
A system is disclosed that includes a first interface port, a second interface port, a signal splitter device, a main booster, and a front-end booster. The signal splitter device may include first, second, and third splitter ports.
Wilson Electronics, Llc
Systems, circuits and methods related to low power efficiency improvement in multi-mode multi-band power amplifiers
Systems, circuits and methods related to low power efficiency improvement in multi-mode multi-band power amplifiers. In some embodiments, a power-amplifier (pa) system can include a first amplification path having one or more pas configured to generate a high power radio-frequency (rf) signal from an input rf signal when in a high power mode.
Skyworks Solutions, Inc.
Efficient power amplification over large operating average power range
Embodiments of a doherty power amplifier that maintain efficiency over a large operating average power range are disclosed. In one embodiment, the doherty power amplifier includes reconfigurable main and auxiliary output matching networks and a fixed combining network.
Telefonaktiebolaget L M Ericsson (publ)
Transformer-based doherty power amplifier
Transformer-based doherty power amplifier (pa). In some embodiments, a doherty pa can include a carrier amplification path having an output that includes a carrier transformer, and a peaking amplification path having an output that includes a peaking transformer.
Skyworks Solutions, Inc.
Method of forming stable states of dense high-temperature plasma
A method is proposed for forming stable states of a dense high-temperature plasma, including plasmas for controlled fusion, the method comprising: generating a dense high-temperature plasma in pulsed heavy-current discharges, followed by injecting the plasma from the area of a magnetic field with parameters corresponding to the conditions of gravitational emission of electrons with a banded energy spectrum and subsequent energy transfer along the spectrum (cascade transition) into the long wavelength region (of ev-energy), this leading to the state of locking and amplification of the gravitational emission in the plasma with simultaneous compression thereof to the states of hydrostatic equilibrium, with using multielectron atoms as a prerequisite element in the composition of a working gas, for quenching the spontaneous gravitational emission from the ground energy levels (the kev-region) of the electron in the proper gravitational field.. .
Zakrytoe Aktisionernoe Obschestvo Rustermosintez
Method and enabling heavy floating touch screen haptics assemblies
A system that includes an actuator amplification apparatus and a push pull actuator disposed on or within the actuator amplification apparatus. The actuator amplification apparatus is configured to receive a push pull actuator.
Methods and uses for molecular tags
Methods and uses for molecular tags are disclosed. Molecular tags may be attached to nucleic acid molecules.
The University Of North Carolina At Chapel Hill
High-voltage to low-voltage low dropout regulator with self contained voltage reference
A circuit and method for providing a temperature compensated voltage comprising a voltage regulator circuit configured to provide a regulator voltage, a voltage reference circuit configured to provide a reference voltage, vref, a comparison circuit configured to provide a control voltage vctl, and an operational amplifier configured to provide amplification and coupling to said comparison circuit, wherein the voltage can be a high voltage greater than 1.2 v.. .
Dialog Semiconductor Gmbh
Method for manufacturing organic processing fluid for patterning of chemical amplification type resist film, organic processing fluid for patterning of chemical amplification type resist film, pattern forming method, manufacturing electronic device, and electronic device
There is disclosed a method for manufacturing an organic processing fluid for patterning of a chemical amplification type resist film, comprising a step of causing a fluid containing an organic solvent to pass through a filtration device having a fluid input portion, a fluid output portion, and a filtration filter film provided in a flow path that connects the fluid input portion and the fluid output portion with each other, wherein an absolute value (. .
Novel reagents for directed biomarker signal amplification
Described herein are methods, compositions and articles of manufacture involving neutral conjugated polymers including methods for synthesis of neutral conjugated water-soluble polymers with linkers along the polymer main chain structure and terminal end capping units. Such polymers may serve in the fabrication of novel optoelectronic devices and in the development of highly efficient biosensors.
Analytical instrument systems
The invention provides optical instrument systems and methods for analyzing signals from biological arrays, and performing analytical amplification reactions for identifying the presence or absence of a target nucleic acid sequence in a sample to be analyzed.. .
Nvs Technologies, Inc.
Massively multiplexed rna sequencing
A method for parallel sequencing target rna from samples from multiple sources while maintaining source identification is provided. The method includes providing samples of rna comprising target rna from two or more sources; labeling, at the 3′ end, the rna from the two or more sources with a first nucleic acid adaptor that comprises a nucleic acid sequence that differentiates between the rna from the two or more sources; reverse transcribing the two or more sources to create a single stranded dna comprising the nucleic acid sequence that differentiates between the rna from the two or more sources; amplifying the single stranded dna to create dna amplification products that comprise the nucleic acid sequence that differentiates between the rna from the two or more sources; sequencing the dna amplification products thereby parallel sequencing target rna from samples from multiple sources while maintaining source identification..
President And Fellows Of Harvard College
Compositions, methods, and kits for amplifying nucleic acids
The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher.
Applied Biosystems, Llc
Polynucleotides for the amplification and detection of chlamydia trachomatis and neisseria gonorrhoeae
Polynucleotides useful for detecting chlamydia trachomatis and/or neisseria gonorrhoeae in a test sample, kits, a nucleic acid amplification method and detection method including the same.. .
Abbott Molecular Inc.
Compositions and methods for the detection and analysis of mycobacterium tuberculosis
Provided herein are compositions and methods useful for the detection of mtb. In particular, provided herein are kits, reagents, reaction mixtures, and methods involving such for nucleic acid amplification and detection procedures, which specifically and sensitively detect mtb in samples..
Abbott Molecular Inc.
Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
The present invention provides methods of amplifying a target nucleic acid utilizing a circularized template. Circularization may be achieved utilizing a bridging oligonucleotide or an inverter primer.
Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification.
Life Technologies Corporation
Fluorescent compounds and uses thereof
The invention provides compounds, including fluorescent nucleic acid dyes, and methods for use including nucleic acid detection, nucleic acid amplification reactions, and high-resolution melt curve analysis. Further provided are kits, instruments and systems comprising compounds of the invention or adapted for use with compounds of the invention or other nucleic acid dyes..
Components for active pedestrian safety mechanism
A latch assembly for driving a closure panel from a closed panel position to an open panel position. The latch assembly comprises: a latch mechanism having a first latch element for retaining a mating latch component in a latched position and for moving the mating latch component from the latched position to an unlatched position when released, the movement of the first latch element biased towards the unlatched position by a latch biasing element; and an actuation system having: a mechanical energy storage system having a device for storing mechanical potential energy for assisting the latch biasing element in moving the mating latch component from the latched position to the unlatched position; a mechanical advantage system having a plurality of advantage elements for engaging the device, such that movement of the plurality of advantage elements is associated with a trigger force and cooperation of the plurality of advantage elements provides for amplification of the trigger force as an initiating force to release the stored mechanical potential energy; and a trigger system configured to provide the trigger force to release the stored mechanical potential energy..
Magna Closures Inc.
Modular voice amplification system for protective mask
Disclosed is a modular voice amplification system for a protective mask that allows connection of a single amplifier assembly to masks of varying configurations through minimal adjustment of the system. Adapter plates in the form of mounting brackets are provided, each of which is configured for attachment to the single amplifier assembly, but is individually configured for attachment to protective masks of varying configurations through differing attachment mechanisms provided on each adapter plate..
D. Wheatley Enterprises, Inc.
Imaging systems with clear filter pixels
An image sensor may have an array of image sensor pixels arranged in color filter unit cells each having one red image pixel that generates red image signals, one blue image pixel that generate blue image signals, and two clear image sensor pixels that generate white image signals. The image sensor may be coupled to processing circuitry that performs filtering operations on the red, blue, and white image signals to increase noise correlations in the image signals that reduce noise amplification when applying a color correction matrix to the image signals.
Semiconductor Components Industries, Llc
Burst-mode optical amplification apparatus and method thereof
A burst-mode optical amplification apparatus and method is provided. The burst-mode optical amplification apparatus includes a gain saturation signal generator configured to generate a gain saturation signal for gain stabilization based on an incoming input optical signal; a wavelength multiplexer configured to wavelength multiplex the incoming input optical signal and the gain saturation signal; and an optical amplifier configured to amplify both the wavelength-multiplexed input optical signal and the wavelength-multiplexed gain saturation signal.
Electronics And Telecommunications Research Institute
Optical transmitter and optical transmission method
An optical transmitter includes a dummy optical source, a polarized wave beam coupler, and an auto gain control (agc)-system amplifier. The dummy optical source outputs, out of an optical signal in which an optical path signal and an optical packet signal are mixed, a dummy signal having a wavelength identical to that of the optical packet signal.
Fujitsu Telecom Networks Limited
Method for calibrating distortion cancelation in duplex communication systems
A radio with a system for cancelation of noise, distortion, and interference products is calibrated using a test signal. The test signal is inserted in a radio transmit path, amplified, and down-converted.
Optical transmitter and optical transmission method
An optical transmitter includes an amplifying unit, a monitor, an identifying unit, and a controlling unit. The amplifying unit amplifies an optical signal in which an optical packet signal is mixed in optical path signals.
Fujitsu Telecom Networks Limited
A power amplifier includes a class d amplification section and a load current feedback circuit. The class d amplification section includes an input section and a switching section serving as an output stage and switched depending on a signal input to the input section, and outputs current from a power source to a load via the switching section.
Psuedo resistor circuit and charge amplifier
A pseudo resistor circuit and a charge amplifier include a first field effect transistor; a second field effect transistor having electrical characteristics matched with electrical characteristics of the first field effect transistor; and a voltage dividing circuit with terminal of a reference resistor electrically connected to a source terminal of the second field effect transistor. Further, a first operational amplifier with an output terminal is connected to a gate terminal of the first field effect transistor and a gate terminal of the second field effect transistor and in which midpoint voltage of the voltage dividing circuit is input into either an inverting or non-inverting input terminal and reference voltage is input into the other of the inverting and non-inverting input terminal.
Murata Manufacturing Co., Ltd.
Systems and methods related to switchable output stages in power amplifiers
Systems and method related to switchable output stages in power amplifiers. In some embodiments, a power amplifier (pa) circuit can include a driver stage configured to amplify a radio-frequency (rf) signal.
Skyworks Solutions, Inc.
Electronically controllable groups of artificially structured unit cells providing localized b1 magnetic fields for mri and nmr devices
Described embodiments include a system, apparatus, and method. A system includes an array of at least two groups of at least two artificially structured electromagnetic unit cells.
Detection of genomic rearrangements by sequence capture
Provided herein is a method of sample analysis. In some embodiments, the method comprises hybridizing fragmented genomic dna from a test genome with a population of first oligonucleotides of the formula v1-b-v2 in the presence of one or more second oligonucleotides; contacting the product with ligase to join the ends of the fragmented genomic dna that are hybridized to v1 and v2 to the one or more second oligonucleotides; and subjecting the product to polymerase chain reaction conditions using amplification primers that hybridize to sites that are provided by the one or more second oligonucleotides, wherein production of a product indicates that the test genome contains a chromosomal rearrangement relative to the reference genome..
Agilent Technologies, Inc.
Systems and methods for monitoring the amplification and dissociation behavior of dna molecules
The present invention relates to systems and methods for monitoring the amplification of dna molecules and the dissociation behavior of the dna molecules.. .
Canon U.s. Life Sciences, Inc.
Reagents and kit compositions for single-cell whole genome amplification
The present invention provides reagents, kits, and methods for single-cell whole genome amplification using phi 29 dna polymerase.. .
Methods for amplification of nucleic acids on solid support
The present invention provides methods for amplifying a nucleic acid from a sample containing a mixture of nucleic acids utilizing a solid support. Methods are provided utilizing user-defined primer oligonucleotides for directional amplification that assists in further manipulation of the target nucleic acid, such as sequencing.
Methods and products for producing engineered mammalian cell lines with amplified transgenes
Methods of inserting genes into defined locations in the chromosomal dna of cultured mammalian cell lines which are subject to gene amplification are disclosed. In particular, sequences of interest (e.g., genes encoding biotherapeutic proteins) are inserted proximal to selectable genes in amplifiable loci, and the transformed cells are subjected to selection to induce co-amplification of the selectable gene and the sequence of interest.
Precision Biosciences, Inc.
Methods for one step nucleic acid amplification of non-eluted samples
The present invention relates to methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. For easy processing and amplification of nucleic acid samples, the samples are bound to a solid support and used directly, without purification, in a nucleic acid amplification reaction such as the polymerase chain reaction (pcr)..
Ge Healthcare Uk Limited
Method for using heat-resistant mismatch endonuclease
Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.. .
Takara Bio Inc.
Droplet-based nucleic acid amplification method and apparatus
Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus.
Advanced Liquid Logic, Inc.
Heterogeneous catalysts for nmr/mri enhancement via signal amplification by reversible exchange (sabre)
Heterogeneous iridium catalysts for nuclear spin polarization enhancement in solution via signal amplification by reversible exchange are provided. Also provided are methods for preparing heterogeneous iridium catalysts, and methods of using heterogeneous iridium catalysts for nuclear spin polarization enhancement in solution via signal amplification by reversible exchange..
Board Of Trustees Of Southern Illinois University
Integrated analysis system
The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing.
Circuit and detection and compensation of transistor mismatch
The present disclosure relates to a detection circuit formed as part of an integrated circuit. In one example, the detection circuit includes a signal generator configured to generate a reference signal, and an amplification circuit comprising a p-channel transistor and an n-channel transistor, wherein the amplification circuit is affected by a variability that also affects a functional circuit formed as part of the integrated circuit.
Stichting Imec Nederland
Circuits and devices related to fast turn-on of radio-frequency amplifiers
Circuits, methods and devices are disclosed, related to fast turn-on of radio-frequency (rf) amplifiers. In some embodiments, an rf amplifier circuit includes an amplification path implemented to amplify an rf signal, where the amplification path includes a switch and an amplifier.
Skyworks Solutions, Inc.
High power spin torque oscillator integrated on a transistor
Example embodiments are related to a high power spin torque oscillator that is integrated by combining a transistor. The high power spin torque oscillator according to example embodiments may include a spin torque oscillator and a transistor.
Korea Advanced Institute Of Technology
High power chirally coupled core optical amplification systems and methods
An optical amplification system is disclosed which includes a seed source providing a seed beam, and a chirally coupled core fiber amplifier optically coupled to the seed beam and configured to convert the coupled seed beam into an amplifier output beam, wherein the polarization of the seed beam is controllably launched into the chirally coupled core fiber in order to reduce nonlinearities in the amplifier output beam. Peak output powers in excess of 500 kw can be realized for short-pulsed single-mode beams having mode field diameters greater than 30 μm..
Nlight Photonics Corporation
Tdm- and wdm-based fbg sensor array system
In a tdm- and wdm-based fbg sensor array system, a source emits a light covering a selected wavelength range. The light is amplified and then used to generate a series of pulses that are fed into an array of sensor gratings.
Ofs Fitel, Llc
Multiplexed kras mutation detection assay
Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the kras gene that contain any of the 34a, 34c, 34t, 35a, 35c, 35t or 38a point mutations. Methods that employ the reagent mix and kits for performing the same are also provided..
Exact Sciences Corporation
The present invention provides methods of reducing the levels of a titratable selectable pressure required, the number of amplification cycles, and the time taken to generate protein expressing cell lines by altering the codons of the desired open-reading-frames. Through the use of codon adaptation for this purpose the methods of the invention consistently provide sufficient yields in faster time frames saving many weeks in cell line development activities.
Glaxo Group Limited
Self-balanced high-pressure and high-shear autoclave and the application in the preparation of layered double hydroxides
The present invention belongs to the synthesis technology field of inorganic functional materials, and particularly provides a self-balanced high-pressure and high-shear autoclave and its application in the preparation of layered double hydroxides (ldhs). In this invention, by imbedding the handpiece of emulsification mill into the autoclave, and by taking the motor driving system outside of the autoclave, the pressure of the autoclave can be highly stable by the use of self-balanced seal gland.
Jiangyin Ruifa Chemical Co., Ltd.
Methods and compositions for paper-based and hybrid microfluidic devices integrated with nucleic acid amplification for disease diagnosis
Certain embodiments are directed to paper and its hybrid microfluidic devices integrated with nucleic acid amplification for simple, cost-effective, rapid, and sensitive pathogen detection, especially in low-resource settings.. .
University Of Texas At El Paso
Liquid ejecting device
A liquid ejecting device includes a driving waveform signal generating circuit that generates a driving waveform signal, a modulation circuit that performs pulse modulation on the driving waveform signal to generate a modulation signal, a digital power amplifier that amplifies power of the modulation signal to generate a power amplification modulation signal in the form of a pulse wave, a filter that smoothes the power amplification modulation signal in the pulse wave to generate the driving signal, a connection cable that connects the filter to the capacitive load and is provided such that at least one of the filter and the capacitive load is detachable, a connection line information acquiring unit that acquires connection line information associated with the connection cable, and a frequency changing unit that changes a frequency when the modulation circuit performs the pulse modulation on the driving waveform signal, on the basis of the connection line.. .
Seiko Epson Corporation
Signal detection device, signal detection method, and manufacturing signal detection device
A signal detection device includes: multiple electrodes that are arranged to come into contact with a subject that generates a signal; an electrode signal selection unit that alternatively selects one signal from signals on the multiple electrodes based on a selection signal; an amplification unit that amplifies the signal that is selected by the electrode signal selection unit; and a flexible substrate on which the multiple electrodes, the selection unit, and the amplification unit are formed, in which the amplification unit is formed on the substrate to form a laminated structure together with the multiple electrodes and the selection unit.. .
Japan Science And Technology Agency
Pluggable optical transceivers with integrated electronic dispersion compensation
Integrated performance monitoring (pm); optical layer operations, administration, maintenance, and provisioning (oam&p); alarming; amplification, and the like is described in optical transceivers, such as multi-source agreement (msa)-defined modules. A pluggable optical transceiver defined by an msa agreement can include advanced integrated functions for carrier-grade operation which preserves the existing msa specifications allowing the pluggable optical transceiver to operate with any compliant msa host device with advanced features and functionality, such as forward error correction (fec), framing, and oam&p directly on the pluggable optical transceiver.
Menara Networks, Inc.
Monitor circuit, semiconductor integrated circuit, semiconductor device, and controlling power supply voltage of semiconductor device
A monitor circuit includes a reference voltage generating unit that generates first and second reference voltages, a first amplifier unit that amplifies a differential voltage between the first reference voltage and the second reference voltage, a second amplifier unit that amplifies a differential voltage between an internal power supply voltage being supplied to a functional block provided in the semiconductor integrated circuit and the first reference voltage, and a comparator unit that compares an amplification result of the first amplifier unit with an amplification result of the second amplifier unit and outputs a comparison result as a measurement result.. .
Renesas Electronics Corporation
Oscillator and semiconductor device including the same
An oscillator includes a flip-flop that outputs a first signal having a phase that is inverted according to a comparison signal, a comparison circuit that compares an amplification voltage with a first reference voltage and outputs the comparison signal, a current source having an output voltage that is fixed to a second reference voltage, and an amplification circuit that generates the amplification voltage according to a current outputted from the current source and the second reference voltage.. .
Industry-academic Cooperation Foundation, Yonsei University
Thin film transistor detection systems and related methods
Some embodiments include a system. The system includes a sensor device having a sensor element having a sensor output and an amplification element having at least one amplification stage, an amplifier input, and an amplifier output.
Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Ariz
Alternating current detector
An alternating current detector is provided. The alternating current detector comprises a housing, a solenoid, a rectification and filtration circuit, an amplification and driving circuit.
K-line Automation Co., Ltd.
Plasmonic nanocavity array sensors for analyte detection enhancement and methods for making and using of the same
This disclosure provides, among other things, a nanosensor for sensing an analyte. In some embodiments the nanosensor comprises (a) a substrate; (b) a signal amplification layer comprising: (i) a substantially continuous metallic backplane on the substrate; (ii) one or a plurality of pillars extending from the metallic backplane or from the substrate through holes in the backplane; and (iii) a metallic disk on top of the pillar, wherein at least one portion of the edge of the disk is separated from the metallic backplane; and (c) a capture agent that specifically binds to the analyte, wherein the capture agent is linked to the surface of the signal amplification layer; wherein the nanosensor amplifies a light signal from an analyte, when the analyte is bound to the capture agent.
The Trustees Of Princeton University
Methods for amplifying nucleic acids on substrates
A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; applying an aqueous buffer to the sample application zone of the substrate to washes away one or more inhibitors present on the sample application zone; and applying an isothermal nucleic acid amplification reaction mixture to the sample application zone to amplify the target nucleic acid to form a nucleic acid amplification product. The target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight..
General Electric Company
Multiphase nucleic acid amplification
Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification.
Mutliplexed in situ molecular analyses and programmable molecular probes for regulated amplification
The present invention generally relates to methods for detecting a target in a sample; methods for modulating the reporting intensity of a labeled target in a sample of fixed cells or tissues; methods for detecting the location of at least two targets in a sample; and related compositions.. .
William Marsh Rice University
Proximity assay for in situ detection of targets
A proximity detection method is described that utilizes enzymatic biotinylation to detect targets in a sample, particularly formalin fixed paraffin embedded samples using automated staining platforms. One disclosed embodiment comprises contacting the sample with a first conjugate comprising a biotin ligase and a first specific binding moiety that binds proximally to the first target; contacting the sample with a second conjugate comprising a biotin ligase substrate and a second specific binding moiety that binds proximally to the second target; subjecting the sample to conditions that allow biotinylation of the biotin ligase substrate by the biotin ligase when the first target and the second target have a proximal arrangement; and detecting biotinylation of the biotin ligase substrate.
Ventana Medical Systems, Inc.
Method, substrate and device for separating nucleic acids
A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided..
General Electric Company