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Nucleic acid amplification method

Nucleic acid amplification method

Date/App# patent app List of recent Amplification-related patents
 Methods for multiplex amplification patent thumbnailMethods for multiplex amplification
Methods for multiplex amplification of target nucleic acid sequences are provided.. .
 Base for use in amplification of nucleic acid and method for amplifying nucleic acid patent thumbnailBase for use in amplification of nucleic acid and method for amplifying nucleic acid
The present invention is to provide a base for use in the amplification of a nucleic acid, which makes it possible to accurately and effectively amplify and detect a plurality of target nucleic acid sequences that are subjects for detection. A base for use in the amplification of a nucleic acid according to the present invention is a base in which a hydrophilic gel is held and which has arrayed plural zones, and the zones contain different kinds of primer or the like for amplifying nucleic acids..
 Nucleic acid amplification method patent thumbnailNucleic acid amplification method
A nucleic acid amplification method includes ligating a double-stranded adapter (20) containing adapter dna strands capable of forming a folded structure to a double-stranded dna (1, 2) containing a target dna sequence (1) to prepare a cyclic dna template composed of double-stranded dna containing a nick (5). A 3′-end elongation reaction is performed using a strand-displacement dna polymerase from the nick (5) as an origin, thereby producing a concatemer (29) in which a plurality of the target dna sequences (1) and the adapter dna strands capable of forming the folded structure are linked in series as a single-stranded dna.
 Detection of abl mutant by allele-specific amplification patent thumbnailDetection of abl mutant by allele-specific amplification
The present invention provides a method of detecting for the presence of a mutation in the abl gene coding for the abl t315i mutation, the method comprising: (a) providing a sample comprising an mrna transcript encoding the abl t315i mutation; (b) performing a reverse-transcription reaction to generate a cdna sequence from the mrna transcript; and (c) amplifying a cdna sequence generated in step (b); wherein the reverse-transcription reaction in step (b) is selective for reverse transcription of the mrna transcript encoding the abl t315i mutation over a corresponding alternative abl transcript that does not contain the mutation.. .
 Compositions and reaction mixtures for the detection of nucleic acids from multiple types of human papillomavirus patent thumbnailCompositions and reaction mixtures for the detection of nucleic acids from multiple types of human papillomavirus
Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (hpv) associated with cervical cancer. Methods for detecting multiple hpv types in biological specimens by amplifying hpv nucleic acid sequences in vitro and detecting the amplified products are disclosed..
 Methods of treating cancer patent thumbnailMethods of treating cancer
Methods of treating cancers comprising fgfr1 gene amplification are provided. In some embodiments, the methods comprise administering a fibroblast growth factor receptor 1 (fgfr1) extracellular domain (ecd) and/or an fgfr1 ecd fusion molecule.
 Device for measurement of the profile of very short duration single pulses patent thumbnailDevice for measurement of the profile of very short duration single pulses
Said replication means comprising parametric amplification means (120,140,141,142) for amplifying said replica pulses.. .
 Apparatus and method for controlling an amplification gain of an amplifier, and a digitizer circuit and microphone assembly patent thumbnailApparatus and method for controlling an amplification gain of an amplifier, and a digitizer circuit and microphone assembly
A controller for controlling an amplification gain of an amplifier for amplifying a high dynamic range signal for an analog to digital converter comprises an input interface adapted to receive a representation of the high dynamic range signal and a signal compressor adapted to provide a low amplitude representation of the high dynamic range signal, the low amplitude representation having a lower signal amplitude than the high dynamic range signal. A comparator is adapted to compare the signal amplitude of the low amplitude representation with a predetermined threshold and an output interface is adapted to provide a control signal to the amplifier, the control signal being adapted to control the amplification gain of the amplifier such that the amplification gain is lowered when the signal amplitude of the low amplitude representation exceeds the predetermined threshold..
 Laser system and laser light generation method patent thumbnailLaser system and laser light generation method
A laser system may include: a master oscillator configured to output pulsed laser light; an amplification device for amplifying the pulsed laser light from the master oscillator; a first timing detector configured to detect a first timing at which the master oscillator outputs the pulsed laser light; a second timing detector configured to detect a second timing at which the amplification device discharges; and a controller configured to, based on results of detection by the first timing detector and the second timing detector, control at least one of the first timing and the second timing so that the amplification device discharges when the pulsed laser light passes through a discharge space of the amplification device.. .
 Fiber laser patent thumbnailFiber laser
A fiber laser includes: an amplification optical fiber; a pumping light source configured to emit pumping light to pump the active element of the amplification optical fiber; a first mirror provided on one side of the amplification optical fiber; and a second mirror provided on the other side of the amplification optical fiber. When an optical loss between the first mirror and the second mirror is equal to an optical gain in the amplification optical fiber, a difference between a gain of light having a wavelength at which a gain becomes maximum and a gain of light having a wavelength reflected off the first mirror and the second mirror is 35 db or less in the amplification optical fiber..
Optical amplification device and method of controlling optical module
An optical amplification device includes: an optical module that outputs an amplified light; and a controller that makes the optical module emit a light when an emission command is input into the controller, wherein the controller cancels an inputting of the emission command until a predetermined time passes, when a protection for forbidding a light emission of the optical module is canceled.. .
Gyrolaser with optimized ignition
A gyrolaser comprises: a ring-shaped optical cavity and a gaseous medium, and at least three electrodes in contact with the gas of the amplification medium, the electrodes generating charges when ignition voltage is applied; the cavity and distribution of the electrodes comprising at least one plane of symmetry perpendicular to the plane of the cavity and passing through the electrode of first type; at least one conductive ignition element set at a predetermined potential, the shape and arrangement being such that symmetry is maintained; the electrically conductive element generating an electric field locally for guiding the charges so they are distributed symmetrically in a first flow and second flow in the first and second discharge areas respectively when the ignition voltage is applied, in such a way that a first plasma and a second plasma are initiated simultaneously, respectively, in the first discharge area and in the second discharge area.. .
Automatic gain control of a received signal using a power target
The present invention relates to a method and device for performing automatic gain control of a received signal. The method comprises the steps of receiving (s101) the signal and amplifying the received signal on the basis of a difference between a power reference value and actual power of the amplified signal.
Amplifier circuit and amplification method
A true ground amplifier circuit in which a voltage sensor senses the output voltage and generates a binary output which indicates whether the output is above or below a threshold. A variable gain feedback system generates a feedback signal for combination with the digital input, thereby to provide offset cancellation.
Devices and methods for monitoring and controlling temperature in a microfluidic environment
The present invention provides improved methods that allow accurate monitoring and/or control of temperature changes in a microfluidic environment. An advantage of the present invention is that the temperature can be monitored and/or controlled at any location within a microfluidic device, especially where a preparation step, an amplification step and/or a detection step is performed.
Thermoacoustic engine
Disclosed is a thermoacoustic engine having: resonance pipes including a working gas; motors; and a branch pipe, where each of the motors has a regenerator, a heater, and a cooler, a temperature gradient is given between both ends of the regenerator to generate self-excited oscillation of the working gas, a channel cross-sectional area of the resonance pipe that is coupled to the heater is expanded by a same amplification factor of a work flow based on the self-excited oscillation or by an amplification factor within a range of ±30% of the amplification factor of the work flow to a channel cross-sectional area of a resonance pipe that is coupled to the cooler, and a channel cross-sectional area of the regenerator is set by 4 to 36 times of the channel cross-sectional area of the resonance pipe that is coupled to the cooler.. .
System and method for outcome tracking and analysis
Provided are systems and methods for capturing outcome information associated with various cancer treatments. The system facilitates capture and analysis of cancer treatment information and associated outcome information.
Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
Provided are devices comprising multivolume analysis regions, the devices being capable of supporting amplification, detection, and other processes. Also provided are related methods of detecting or estimating the presence nucleic acids, viral levels, and other biological markers of interest..
Method and kit for characterizing rna in a composition
V. Sequencing the amplification products by means of next generation sequencing..
Apparatus for regulating transmission power of repeater in long term evolution system
The present invention relates to an apparatus capable of flexibly regulating coverage of a repeater in a long term evolution system (hereinafter referred to as an lte system). The present invention provides an apparatus for regulating transmission power of a repeater in an lte (long term evolution) system, the apparatus including a sink signal detector that detects a sink signal of a reception signal received through an if/rf receiver of the repeater, a sink signal power measurer that measures a size of power of the sink signal detected through the sink signal detector, and an amplification controller that calculates a gain of an amplifier of the repeater for maintaining coverage of the repeater based on the size of the power of the sink signal measured through the sink signal power measurer, and controls the amplifier of the repeater according to the calculated gain.
Method for signal amplification in biosensor-based system for rapidly detecting infectious agents
A system for detecting infectious agents in biological samples in real time that includes a sample to be tested for at least one specific infectious agent; and a biosensor, wherein the biosensor is operative to detect a specific infectious agent in the sample to be tested; and wherein the biosensor emits an amplified and detectable signal when it reacts with the specific infectious agent. Signal detection is enhanced through the release of lps from the cells of the infectious agent..
Apparatus for detecting nucleic acid amplification product in real time
There is provided an apparatus for detecting a nucleic acid amplification product in real time, which is capable of effectively excluding or reducing apparatus error factors without using a second fluorescence signal used for correction. A plurality of wells 7a are given with temperature cycles and fluorescence strength from a nucleic acid amplification product is detected in real time in each well 7a.
Analyte enrichment methods and compositions
Methods and compositions for enriching a population of particles containing an analyte are disclosed. In one embodiment, enrichment beads are used that are larger in size than the beads used for amplification.
Direct nucleic acid analysis
The present disclosure provides methods, systems, and apparatuses for collecting and/or amplifying nucleic acids. In general, provided methods, systems, and apparatuses involve contacting a sample including a nucleic acid with a nucleic acid amplification reagent without purification of nucleic acids from the sample..
Methods for detecting nucleic acid sequence variants
The present invention provides methods for detecting the presence or absence of a nucleic acid variant in a target region. These methods include amplifying the target region with a forward primer and a reverse primer in the presence of a selector blocker.
Dual bridge amplifier configuration
An amplifier has a dual bridge design with two bridge amplifiers. A mode switch enables them to be configured in a series amplification mode.
Methods and apparatus for all digital multi-level outphasing using bandwidth reduction
For use in a transmitter in a wireless network, a transmitter apparatus is configured for digital multi-level outphasing. The apparatus includes a bandwidth reduction (bwr) modulator block configured to receive a phase modulated carrier and reduce a bandwidth of the phase modulated carrier using amplitude modulation.
Method and device for the optical parametric amplification of pulses with frequency drift
According to the invention, which is particularly applicable to laser-matter interaction, a four-wave mixing effect is used, preferably in an optical fibre (f), between each pulse (s) and the two pump signals (p1, p2); and the half-sum (fm) of the respective optical frequencies (fp1, fp2) of these signals pertains to the pulse spectrum support.. .
Surface plasmon resonance biosensor
An optical biosensor is provided for detecting a bio-molecular sample by goos-hänchen (gh) enhancement of aharonov-albert-vaidman (aav) amplification to a surface plasmon resonance (spr) detector. The sensor includes pre- and post-selection polarizers respectively upstream and downstream of a right-isosceles prism with a metal film and a liquid medium disposed on a diagonal side of the prism.
Optical parametric amplification of weak signals for imaging biological tissue
Methods and apparatus for obtaining an image of light scattering biological tissue. A series of pulses of substantially monochromatic light of a first wavelength is generated and split into two parts, of which one part illuminates a scattering biological tissue at an intensity too low to damage the tissue, while a second part is upconverted to generate a pump beam.
Photon counting controller, radiographic imaging apparatus, and control method of photon counting controller
The photon counting controller includes an amplification controller to charge an input electrical signal, to amplify the input electrical signal, and to discharge the charged electrical signal, a charge controller to control a charge and a discharge of the electrical signal of the amplification controller based on a received feedback signal, and a measuring controller to compare voltage of the amplified electrical signal with reference voltage and to count photons based on a result of comparison.. .
Laser scanning code symbol reading system
A laser scanning symbol reading system includes an analog scan data signal processor for producing digital data signals, wherein during each scanning cycle, a light collection and photo-detection module generates an analog scan data signal corresponding to a laser scanned symbol, a multi-channel parallel scan data signal processor/digitizer processes the analog scan data signal along multiple cascaded multi-stage signal processing channels, to generate digital data signals corresponding thereto, while a synchronized digital gain control module automatically processes the digital data signals in response to start of scan (sos) signals generated by a sos detector. Each signal processing channel supports different stages of amplification and filtering using a different set of band-pass filtering and gain parameters in each channel, to produce multiple digital first derivative data signals, and/or multiple digital scan data intensity data signals, having different signal amplitudes and dynamic range characteristics for use in decode processing..
Electromagnetic wave treatment method and promoter
A method involving exposing a substance to a promoter, the promoter being made from a liquid carrier and a metal salt component, the metal salt component, having at least (i) a magnetic susceptibility above 1000 or (ii) an ionization potential below about 500 volts, or (iii) both (i) and (ii), and applying electromagnetic wave energy to the substance while the substance is in the presence of the promoter, for a period of time and at a frequency and amplification sufficient to promote the modification of at least one physical property of the substance. A related method of treating a well in a subterranean formation, a tank or a pipeline, and the well treatment promoter composition, are also described..
Method of amplifying a nucleic acid
A nucleic acid amplification method is provided, along with kits useful in performing the amplification method.. .
Constructing method of high-throughput sequencing library and use thereof
The present invention provides a method for constructing a high-throughput sequencing library, which comprises: fragmenting genomic dna; end-repairing the dna fragments; adding a base a to the 3′ end of the end-repaired dna fragments; connecting the dna fragments having cohesive end a with a methylated adapter; carrying out hybrid capture on the connection products by using specific probes to obtain object fragments; treating the object fragments with bisulfite, to convert non-methylated cytosines to uracils; pcr amplifying the converted object fragments; and separating and purifying the amplification products, wherein the amplification products constitute the high-throughput sequencing library. The present invention also provides a method and an apparatus for identifying methylation information in specified genome regions of a sample..
Devices, methods, and systems for nucleic acid amplification
A microfluidic device comprising a first surface portion and a first sample retaining element, which have differing affinities to a fluid, and a method comprising supplying a sample to such a device. In some embodiments, the differing affinity is a result of plasma, ion embedding, surface charging, chemical, optical, electronic and/or electromagnetic treatment.
Multiplexed diagnostic detection apparatus and methods
Disclosed herein are devices configured for the amplification and detection of multiple targets from a sample, and methods of using the same. The devices disclosed herein comprise microfluidic cartridges have a first stage (amplification) and a second (detection) stage.
Biosensor using redox cycling
The present invention relates to a biosensor using dual amplification of signal amplification by means of enzymes coupled with signal amplification by means of redox cycling, and to the use of a technology that maintains a slow reaction state between redox cycling materials without the use of redox enzymes, and induces quick chemical-chemical redox cycling. In addition, the present invention relates to a biosensor which obtains triple amplification by inducing electrochemical-chemical-chemical redox cycling, in addition to signal amplification by means of enzymes and signal amplification by means of chemical-chemical redox cycling..
Methods for multiplexing amplification reactions
A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step.
Amplification methods to minimise sequence specific bias
Methods for amplifying nucleic acids are provided. The methods can be used to minimise sequence specific bias caused by the preferential amplification of certain nucleic acid sequences.
Apparatus for amplification of nucleic acids
Described herein is a chip-based apparatus for amplifying nucleic acids, a cartridge housing the apparatus, and methods of using the apparatus for amplification of nucleic acids. More specifically, this invention provides integrated semiconductor chip, manufactured with standard semiconductor manufacturing process, with on-chip circuitry to perform thermal management and optical sensing necessary for amplification of nucleic acids.
Chip-based sequencing nucleic acids
A system for fast dna sequencing by amplification of genetic material within microreactors, denaturing, demulsifying, and then sequencing the material, while retaining it in a pcr/sequencing zone by a magnetic field. One embodiment includes sequencing nucleic acids on a microchip that includes a microchannel flow channel in the microchip.
Method for the detection of nucleic acid synthesis and/or amplification
The present invention relates to a method for the detection of nucleic acid synthesis and/or amplification, characterised in that the method includes adding at least one colorimetric metal indicator and at least one bland magnesium chelator to a reaction mixture for nucleic acid amplification. The present invention further relates to a kit for carrying out such a method and to the use thereof in the health, food and agricultural or veterinary fields..
Simplified gating method for sealing and flow control in micro and nano devices
A biochip for multiplex genetic identification is disclosed. An biochip for separating and detecting a plurality of dna fragments includes a set of inputs and chambers for receiving a sample matrix of genetic material and reagents needed to conduct a polymerase chain reaction amplification of the genetic material.
Optical transceiver
A gain medium (7) arranged in the amplification section of the first waveguide and suitable for producing a stimulated light transmission, and an optical detector (8) coupled to the reception section of the second waveguide.. .
Laser apparatus
When a chamber of an oscillator and one or more amplifiers is to be replaced at the timing when a predetermined period elapses, one of the chambers of the oscillator and the amplifiers having a low allowable deterioration limit is detached and this chamber is attached in place of one of the chambers of the oscillator and the amplifiers other than the one having the low allowable deterioration limit. Thus, the chamber which has been used in one of the oscillator and amplifiers having a low allowable deterioration limit and has not reached the limit of deterioration is reused in one of the oscillator and the amplifiers other than the one having the low allowable deterioration limit.
Imaging device, method for controlling imaging device, and storage medium storing a control program
An imaging apparatus (10) includes: an image pickup device (14) including plural photoelectric conversion elements arrayed in first and second directions; an amplification means that treats (k×l) pixels as respective common pixels and amplifies an image capture signal for each of the common pixels; a color filter that is provided with a repeatedly disposed basic array pattern or first and second filters placed in a predetermined pattern of (n×m) pixels; and an image processing section (20) and a drive section (22) that perform charge sweeping by applying sequential shutter pulses to the image pickup device (14) in turn to each pixel, or to each scan line, or to each of plural pixels on a scan line, that read pixel signals of the plural pixels at a set cycle, that, from the read pixel signals, generate line image data of pixels that are arrayed running along the second direction at a cycle of from k pixels to n pixels in the first direction, and that generate image data based on the line image data.. .
Systems and methods of rf power transmission, modulation, and amplification, including control functions to transition an output of a miso device
Embodiments of the present invention include a method and system for control of a multiple-input-single output (miso) device. For example, the method includes partitioning a waveform constellation space into a plurality of regions, where each region of the plurality of regions is associated with one or more control functions of the miso device.
Instrumentation amplifier with rail-to-rail input range
A system and method for adjusting a common mode output voltage in an instrumentation amplifier is provided. In one aspect, the common mode output voltage is increased or decreased with respect to the common mode input voltage to enable high amplification of the signal input to the instrumentation amplifier.
Flash translation layer with lower write amplification
A method of associating a logical block address with a physical location in a non-volatile memory includes (a) in response to a write request comprising a respective logical block address in a logical block address space and respective data to be written to the non-volatile memory, determining a physical location in the non-volatile memory to store the respective data of the write request, (b) adding an entry to a journal, such that the added entry trails any entries already in the journal and the added entry has a respective logical block address field set to the respective logical block address of the write request and a respective physical location field set to the determined physical location, and (c) updating one of a plurality of second-level map pages in a two-level map according to the respective logical block address of the write request with the determined physical location.. .
Voice input/output device, method and programme for preventing howling
A voice separation means 82 separates an input voice of a volume adjusted by an input volume adjustment means 81, into a voice recognition voice and a monitoring voice. A monitoring volume adjustment means 83 adjusts a volume of the monitoring voice.
Methods and apparatus for the control of adenosine triphosphate synthase activity within living organisms, and conditioning of water-based fluids and substances using magnetic field exposures or their withdrawal
The present invention relates to the control by magnetic fields (mf) of the activity of the mitochondrial enzyme adenosine triphosphate synthase (atps). Elimination of alternating and static mfs allows atps activity amplification (atpsa), while a variety of mf frequency-intensity combinations interfere to various degrees with atps function (atpsi).
Transmitter with class e amplifier
According to one embodiment, a transmitter includes a first buffer, a second buffer, a logic circuit, and a class e power amplifier. The first buffer receives a first sinusoidal signal, and converts the first sinusoidal signal to a first rectangular wave signal.
Nanocrystal based biomolecule detection
A new signal amplification method exploiting the dense atom packing in metallic nanocrystals has been developed for detecting target substances. By dissolving nanocrystals to individual ions that are stoichiometrically converted to chromophores and quantified photometrically, extremely high signal amplification can be achieved.
Method of preparing sample for nucleic acid amplification reaction, nucleic acid amplification method, and reagent and microchip for solid phase nucleic acid amplification reaction
Provided is a method of preparing a sample for nucleic acid amplification reaction, including: a procedure of dissolving a solid phase reagent at least containing dna polymerase, cyclodextrin, and a binder, in a liquid containing a nucleic acid.. .
Phi29 dna polymerase mutants having increased thermostability and processivity
Mutants of bacteriophage phi29 dna polymerase with increased protein stability and increased half-life, compared to wild type dna polymerase. The disclosed mutants are more stable in reaction mixtures with or without dna.
Method for the production of human thrombin and uses thereof
The present invention disclosed the cloning and the purification of a mutant of prethrombin-2, which contains a thrombin cleavage site instead of a factor xa cleavage site. The stable mutant prethrombin-2 is able to convert itself autocatalytically into active α-thrombin in the absence of ecarin or factor xa.
Selective amplification and detection of mutant gene alleles
The present invention provides methods for selectively amplifying a mutant allele of a gene. These methods include (a) contacting a sample containing a mutant and a normal allele of a gene with at least one primer that selectively modifies the normal allele of the gene and causes further amplification of the normal allele to fail or to be substantially reduced; and (b) selectively amplifying the mutant allele of the gene or a portion thereof.
Methods of detecting mutations and epigenetic changes
Disclosed are methods for assessing the methylation and mutation status of nucleic acid in a sample. The methods provide for methylation-dependent modification of the nucleic acid in a sample, and subsequently nucleic acid amplification processes to distinguish between mutated and non-mutated target sequence..
Hearing assistance apparatus having single multipurpose control device and method of operation
A hearing assistance device has one multipurpose control device that operates in an algorithm selection mode and a daily use mode. In the algorithm selection mode, the multipurpose control device is used to switch between amplification algorithms and select one of the algorithms to be implemented in an audio processing program.
Optical pulse compressing based on chirped fiber bragg gratings for pulse amplification and fiber lasers
Techniques and devices for using a chirped fiber bragg grating to compress amplified laser pulses.. .
Apparatus and methods for radio frequency signal boosters
Provided herein are apparatus and methods for radio frequency (rf) signal boosters. In certain implementations, a multi-band signal booster is provided for boosting the uplink and downlink channels of at least a first frequency band and a second frequency band.
Solid-state imaging device
According to one embodiment, a solid-state imaging device includes a pixel array unit where pixels are disposed in a matrix and a column amplifying circuit that is disposed at an end of the pixel array unit and amplifies a unit signal of a unit pixel read from each pixel with at least first and second amplification factors, and outputs a plurality of amplified signals.. .
Pipeline analog-to-digital converter
A pipeline analog-to-digital converter is disclosed which includes at least one periodic unit consisting of two adjacent stages that jointly use two capacitor networks of the same structure. Each of the capacitor networks includes two identical capacitors, two switches and four terminals.
Doherty power amplification apparatus and method
A multi-way doherty power amplifier, dpa, is disclosed, comprising a first path comprising a carrier amplifier or at least one carrier amplifier segment partitioned from the carrier amplifier; a second to n-th paths each comprising at least one carrier amplifier segment and/or at least one peaking amplifier segment partitioned from a peaking amplifier; and a power splitter for splitting an input power signal to each of the at least one carrier amplifier segment and/or at least one peaking amplifier segment in a same path, wherein n is an integer not less than 2; a signal preparation unit configured for generating separately input power signal for the first path and each of the second path to n-th paths; and an impedance inverting network configured for combining output signal power from each path. The performance of each amplifier cell can be maximized independently without any compromises made for each other..

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Amplification topics: Amplification, Nucleic Acid, Nucleotide, High Resolution, Modulation, Feedback Signal, Nanoparticle, Semiconductor, Raman Amplifier, Resonance Frequency, User Interface, Transmitter, Methylation, Sample Preparation, Sequencing

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