|| List of recent Amplification-related patents
|Isothermal amplification systems and methods|
The present invention relates to systems and methods for performing isothermal amplification reactions, in particular, denaturation methods for use in isothermal amplification reactions. An exemplary method may comprise: a) contacting a target nucleic acid with an electrode, wherein the electrode surface has a plurality of first and optionally second nucleic acid primers immobilized thereon, and wherein a target nucleic acid hybridizes to at least one of said first and second nucleic acid primers; b) extending at least one of the first and second primers using a dna polymerase to form extended target nucleic acids; c) applying positive electrical bias to the electrode such that the extended target nucleic acids anneal to one of the first and second primers; d) extending the target nucleic acid with a dna polymerase to form amplified target nucleic acid; e) reversing the electrical bias such that the amplified target nucleic acid is denatured from the surface..
|Composition to overcome inhibitors in pcr and growth cultures|
Provided herein are compositions and methods for improving amplification or detection of a target nucleic acid in a sample containing pcr inhibitors, such as polyphenols. One aspect provides an enhancer composition including casein or polyvinylpyrrolidone, or a modified polymer thereof.
|Protective case with speakerphone for digital products|
A digital product protective cover with speakerphone, including a protective cover main body where the main body includes a bottom board and surrounding curved walls, at least one card slot located between the bottom board and curved walls, where each card slot is inter-connected, each card slot includes at least one amplification mechanism, the amplification mechanism includes a sound inlet, a sound outlet, and a sound tube, which connects the sound inlet and sound outlet. The sound inlet is located at the bottom of the card slot, which is in proximity to an external speaker of the digital product, the sound inlet and sound outlet are staggered in the longitudinal direction of the card slot, and the sound inlet and sound outlet are staggered in such a position to extend the length of the sound tube..
|Neutron monitoring system|
A neutron monitoring system for monitoring a state of a fuel used in a nuclear power plant includes the following: neutron detector; a structural material to which the neutron detector is mounted; a water detection sensor mounted at a position corresponding to the same height as is the neutron detector, the water detection sensor including a thermocouple and a heating element; an amplification circuit configured to amplify a neutron monitoring signal sent from the neutron detector; a temperature measuring circuit connected to a thermocouple of the water detection sensor via strands; a heater power supply connected to a heating element of the water detection sensor via heater lead wires; a water existence determining control section configured to determine whether the water detection sensor is underwater; and a correction circuit configured to correct an output signal value of the amplification circuit in accordance with a determination signal from the water existence determining control section.. .
|Tunable system and method for tuning a system|
There is provided a system that includes a tunable amplification module, a coupler, an interface module; a detector that is arranged to output an impedance indicative signal and a power indicative signal; wherein the impedance indicative signal is indicative of an impedance mismatch between an output impedance of the tunable amplification module and an impedance of a radio frequency (rf) antenna as seen by the tunable amplification module, wherein the power indicative signal is indicative of an output power of the tunable amplification module.. .
|Methods for nucleic acid manipulation|
A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor.
|Methods and apparatus for point-of-care nucleic acid amplification and detection|
Methods and apparatus are provided for point-of-care nucleic acid amplification and detection. One embodiment of the invention comprises a fully integrated, sample-to-answer molecular diagnostic instrument that optionally may be used in a multiplexed fashion to detect multiple target nucleic acid sequences of interest and that optionally may be configured for disposal after one-time use.
|Method for transcriptional amplification of nucleic acids combining steps of different temperatures|
A method of transcriptional amplification includes: (a) obtaining a mixture by combining (i) a biological sample comprising nucleic acids, (ii) amplification primers, (iii) amplification reagents including enzymes required for amplification, and (iv) at least one polyol capable of stabilizing the enzymes required for amplification; (b) denaturing the nucleic acids by heating the mixture at a temperature above 41° c.; and (c) transcriptionally amplifying at least one target nucleic acid at a temperature above 41° c. When present in the mixture..
|Fingerprint sensing apparatus|
The light refracting device can, for example, be a tft light panel structure or an active matrix organic light emitting diode (amoled) panel structure with reverse current measurement and amplification circuitry, and includes an imaging surface and a viewing plane. Incident light from the light source is projected directly or indirectly onto the imaging surface to create an image of the patterned object from the projected light onto the viewing plane.
|Configurable pre-emphasis component for transmission circuitry|
A configurable pre-emphasis filter component may be configured based upon measured frequency response (e.g., filter effect) associated with at least one analog filter positioned between a digital predistortion component and a power amplification component of a transmission circuitry. The frequency response may be measured using a calibration signal sent via the transmission circuitry, a loopback circuit, and a reception circuitry.
|Extending network signal range via vehicles|
A controller device, implemented in a repeating system associated with a vehicle, may activate an antenna device included in the repeating system to cause the antenna device to receive a network signal and transmit an amplified network signal, corresponding to the network signal, to an area around the vehicle; and cause an amplification device, included in the repeating system, to receive the network signal, amplify the network signal to form the amplified network signal, and provide the amplified network signal for transmission by the antenna device.. .
|Ultrasonic observation equipment, ultrasonic observation system, and ultrasonic observation method|
A single-element ultrasonic sensor includes a single transducer element and transmits an ultrasonic wave on the basis of a pulse wave. An ultrasonic array sensor includes a plurality of transducer elements and receives an ultrasonic reflected wave.
|Amplitude noise squeezing on multi-amplitude modulated signals|
A method for regenerating optical signals includes determining an input including a source amplitude-modulated optical signal, regenerating the source amplitude-modulated optical signal by using successive saturation modes of amplification, and producing an output optical signal from the regeneration. The source amplitude-modulated optical signal includes input power modulation levels that each indicate information carried on the source amplitude-modulated optical signal.
|Pulse generator and analog-digital converter including the same|
Provided is a pulse generator. The pulse generator includes: a pulse generation unit receiving an analog signal and generating a first pulse signal in response to a comparison result of a voltage level applied to a first node and a reference voltage according to the received analog signal; a pulse amplification unit generating a second pulse signal having a wider pulse width than the first pulse signal according to a comparison result of a voltage level applied to a second node and a ground voltage, in response to the first pulse signal; and a gate logic outputting a final pulse signal as a signal for digital conversion by performing a logic operation on the first and second pulse signals..
A system and method of calibrating an amplifier are presented. The amplifier has a first amplification path and a second amplification path.
|High frequency module and portable terminal using same|
When the frequency bandwidth of a high frequency signal to be amplified is changed, the linearity of a high frequency module deteriorates. A high frequency module has an amplifier circuit including an amplification transistor and a variable impedance circuit, and an output matching network.
|High-frequency signal processing device|
Disclosed is a high-frequency signal processing device capable of reducing transmission power variation and harmonic distortion. For example, the high-frequency signal processing device includes a pre-driver circuit, which operates within a saturation region, and a final stage driver circuit, which operates within a linear region and performs a linear amplification operation by using an inductor having a high q-value.
|Inductive power transmission device|
A power transmission device for inductive energy transfer. The power transmission device comprises a first stage adapted to be connected to a supply input voltage and adapted to convert the supply input voltage to an operating voltage.
|Systems and methods for delivering a therapeutic agent using mechanical advantage|
Devices and methods for delivering a therapeutic agent to a patient are disclosed herein. In one embodiment, a delivery system includes a reservoir containing a fluid and a fluid communicator in fluid communication with the reservoir.
|Multiplex amplification methods|
Compositions and methods for amplifying selected polynucleotides, including dna and rna, particularly in multiplex amplification reactions using common primers amplification. Generally, methods of the invention employ multiple steps such as template-specific hybridization, a linear amplification, partial degradation of nucleic acid, and ligation.
|Method for detecting nucleotide mutation, and detection kit|
Provided are a detection method and detection kit for detecting a nucleotide mutation simply, with highly sensitivity and high specificity, which allow a plurality of samples to be simultaneously detected in a single measurement. The detection method includes the steps of: amplifying a nucleic acid to be tested to obtain a dna amplification product; subjecting the dna amplification product, a first nucleic acid probe having a region complementary to a first region of the dna amplification product, a second nucleic acid probe having a region complementary to a second region of the dna amplification product, and a plurality of kinds of signal amplification probes to be used in a palsar method to a reaction to form a complex for detection including a probe polymer; and detecting the probe polymer bound in the complex for detection to detect the presence or absence of a mutation in a nucleotide mutation site of the dna amplification product, the first region of the dna amplification product including the nucleotide mutation site, the nucleotide mutation site being located at a portion other than both end portions of the first region, the second nucleic acid probe having a base sequence identical to part or all of the base sequence of one of the signal amplification probes..
|Amplification of a sequence from a ribonucleic acid|
The present invention relates to methods and compositions for tagging, amplifying, purifying, and or characterizing of ribonucleic acid (rna) in a sample. In particular, methods are provided for preparing rna from a sample for subsequent analysis..
|Portable high gain fluorescence detection system|
An instrument for fluorometric assays in liquid samples is disclosed. The instrument may include multiple optical channels for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control.
|Portable, low power instrument for the optoelectronic detection of pathogens using isothermal nucleic acid amplification protocols|
This invention relates to a portable, low power apparatus and method for detecting a pathogen of interest in a biological sample obtained from a patient. The apparatus includes a heated pathogen isothermal amplification and diagnostic cell for conducting a loop-mediated isothermal amplification technique.
|Methods of enhancing genetic diagnosis|
The present disclosure relates to the use of laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells.
|Nucleic acid preparation compositions and methods|
Provided herein are methods and compositions to extract and enrich by, physical separation or amplification, relatively short nucleic acids from a nucleic acid composition containing a high background of longer nucleic acids (e.g., host or maternal nucleic acids; genomic nucleic acid and the like).. .
This invention relates to a ceiling mounted air circulating apparatus comprising, in combination: a motor; an essentially centrally located radial or axial air impeller; air guiding means; optional airflow enhancement by means of air amplification means; an optional centrally located wiring path from an electrical power source to a wiring enclosure suitable for hanging a lighting fixture or chandelier; and decorative embellishments, all of which are arranged to blend with the architectural style of a space in a manner similar to a decorative ceiling medallion, the planform of which, when viewed from below may be circular, rectangular, or polygonal. Optionally, the apparatus of the present invention may embody any of the following; heating, cooling, filtration means, positive ion generation means, and/or air sterilization means..
An optical receiver including a waveguide substrate including a first waveguide that transmits a main signal beam, a second waveguide that transmits a monitoring beam that has branched from the main signal beam, and a third waveguide that transmits an amplification beam to amplify the main signal beam; a light receiving device array including, integrally formed to the same substrate, a first light receiving device that detects the main signal beam and a second light receiving device that detects the monitoring beam; and a case that houses the waveguide substrate and the light receiving device array. The first light receiving device faces toward an end of the first waveguide, and the second light receiving device faces toward an end of the second waveguide..
|Optical transmission apparatus and optical transmission method|
An optical transmission apparatus includes: an optical amplifier configured to amplify a wavelength multiplexing light to be transmitted, the wavelength multiplexing light being input thereto; a combining unit configured to combine a plurality of non-signal lights in such a way that angles formed between polarization planes of the plurality of non-signal lights of a wavelength of wavelengths belonging to an amplification band of the optical amplifier become equal, the wavelength being different from a wavelength of a polarization multiplexing signal light; and a wavelength multiplexer configured to generate the wavelength multiplexing light to be input to the optical amplifier by multiplexing the plurality of non-signal lights combined by the combining unit and the polarization multiplexing signal light.. .
|Low disturbance, power-consumption, and latency in nand read and program-verify operations|
A hinand array with a hierarchical-bl scheme configured to divide a large global bit line (gbl) capacitance into j number of small local bit line (lbl) capacitances for reducing bit line precharge voltage and discharge time to achieve faster read and program-verify speed, lower power consumption, lower latency, and lower word line disturbance for a reliable dram-like latch sensing. A reduced precharge voltage can be increased by m-fold (m≧2) using a multiplier between each bitline and each latch sense amplifier (sa).
|Efficient power amplification over large operating average power range|
Embodiments of a doherty power amplifier that maintain efficiency over a large operating average power range are disclosed. In one embodiment, the doherty power amplifier includes reconfigurable main and auxiliary output matching networks and a fixed combining network.
|Compensated optical detection apparatus, systems, and methods|
In some embodiments, apparatus and systems, as well as methods, may operate to receive radiation at an active detector of a pair of radiation detectors to provide a first signal proportional to an intensity of the radiation, to receive none of the radiation at a blind detector of the pair of radiation detectors to provide a second signal proportional to the reception of no radiation, and to combine the first signal and the second signal to provide an output signal representing the difference between the first signal and the second signal. The pair of radiation detectors may comprise thermopile detectors.
|Components for active pedestrian safety mechanism|
A latch assembly for driving a closure panel from a closed panel position to an open panel position. The latch assembly comprises: a latch mechanism having a first latch element for retaining a mating latch component in a latched position and for moving the mating latch component from the latched position to an unlatched position when released, the movement of the first latch element biased towards the unlatched position by a latch biasing element; and an actuation system having: a mechanical energy storage system having a device for storing mechanical potential energy for assisting the latch biasing element in moving the mating latch component from the latched position to the unlatched position; a mechanical advantage system having a plurality of advantage elements for engaging the device, such that movement of the plurality of advantage elements is associated with a trigger force and cooperation of the plurality of advantage elements provides for amplification of the trigger force as an initiating force to release the stored mechanical potential energy; and a trigger system configured to provide the trigger force to release the stored mechanical potential energy..
|Methods for multiplex amplification|
Methods for multiplex amplification of target nucleic acid sequences are provided.. .
|Base for use in amplification of nucleic acid and method for amplifying nucleic acid|
The present invention is to provide a base for use in the amplification of a nucleic acid, which makes it possible to accurately and effectively amplify and detect a plurality of target nucleic acid sequences that are subjects for detection. A base for use in the amplification of a nucleic acid according to the present invention is a base in which a hydrophilic gel is held and which has arrayed plural zones, and the zones contain different kinds of primer or the like for amplifying nucleic acids..
|Nucleic acid amplification method|
A nucleic acid amplification method includes ligating a double-stranded adapter (20) containing adapter dna strands capable of forming a folded structure to a double-stranded dna (1, 2) containing a target dna sequence (1) to prepare a cyclic dna template composed of double-stranded dna containing a nick (5). A 3′-end elongation reaction is performed using a strand-displacement dna polymerase from the nick (5) as an origin, thereby producing a concatemer (29) in which a plurality of the target dna sequences (1) and the adapter dna strands capable of forming the folded structure are linked in series as a single-stranded dna.
|Detection of abl mutant by allele-specific amplification|
The present invention provides a method of detecting for the presence of a mutation in the abl gene coding for the abl t315i mutation, the method comprising: (a) providing a sample comprising an mrna transcript encoding the abl t315i mutation; (b) performing a reverse-transcription reaction to generate a cdna sequence from the mrna transcript; and (c) amplifying a cdna sequence generated in step (b); wherein the reverse-transcription reaction in step (b) is selective for reverse transcription of the mrna transcript encoding the abl t315i mutation over a corresponding alternative abl transcript that does not contain the mutation.. .
|Compositions and reaction mixtures for the detection of nucleic acids from multiple types of human papillomavirus|
Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (hpv) associated with cervical cancer. Methods for detecting multiple hpv types in biological specimens by amplifying hpv nucleic acid sequences in vitro and detecting the amplified products are disclosed..
|Methods of treating cancer|
Methods of treating cancers comprising fgfr1 gene amplification are provided. In some embodiments, the methods comprise administering a fibroblast growth factor receptor 1 (fgfr1) extracellular domain (ecd) and/or an fgfr1 ecd fusion molecule.
|Device for measurement of the profile of very short duration single pulses|
Said replication means comprising parametric amplification means (120,140,141,142) for amplifying said replica pulses.. .
|Apparatus and method for controlling an amplification gain of an amplifier, and a digitizer circuit and microphone assembly|
A controller for controlling an amplification gain of an amplifier for amplifying a high dynamic range signal for an analog to digital converter comprises an input interface adapted to receive a representation of the high dynamic range signal and a signal compressor adapted to provide a low amplitude representation of the high dynamic range signal, the low amplitude representation having a lower signal amplitude than the high dynamic range signal. A comparator is adapted to compare the signal amplitude of the low amplitude representation with a predetermined threshold and an output interface is adapted to provide a control signal to the amplifier, the control signal being adapted to control the amplification gain of the amplifier such that the amplification gain is lowered when the signal amplitude of the low amplitude representation exceeds the predetermined threshold..
|Laser system and laser light generation method|
A laser system may include: a master oscillator configured to output pulsed laser light; an amplification device for amplifying the pulsed laser light from the master oscillator; a first timing detector configured to detect a first timing at which the master oscillator outputs the pulsed laser light; a second timing detector configured to detect a second timing at which the amplification device discharges; and a controller configured to, based on results of detection by the first timing detector and the second timing detector, control at least one of the first timing and the second timing so that the amplification device discharges when the pulsed laser light passes through a discharge space of the amplification device.. .
A fiber laser includes: an amplification optical fiber; a pumping light source configured to emit pumping light to pump the active element of the amplification optical fiber; a first mirror provided on one side of the amplification optical fiber; and a second mirror provided on the other side of the amplification optical fiber. When an optical loss between the first mirror and the second mirror is equal to an optical gain in the amplification optical fiber, a difference between a gain of light having a wavelength at which a gain becomes maximum and a gain of light having a wavelength reflected off the first mirror and the second mirror is 35 db or less in the amplification optical fiber..
|Optical amplification device and method of controlling optical module|
An optical amplification device includes: an optical module that outputs an amplified light; and a controller that makes the optical module emit a light when an emission command is input into the controller, wherein the controller cancels an inputting of the emission command until a predetermined time passes, when a protection for forbidding a light emission of the optical module is canceled.. .
|Gyrolaser with optimized ignition|
A gyrolaser comprises: a ring-shaped optical cavity and a gaseous medium, and at least three electrodes in contact with the gas of the amplification medium, the electrodes generating charges when ignition voltage is applied; the cavity and distribution of the electrodes comprising at least one plane of symmetry perpendicular to the plane of the cavity and passing through the electrode of first type; at least one conductive ignition element set at a predetermined potential, the shape and arrangement being such that symmetry is maintained; the electrically conductive element generating an electric field locally for guiding the charges so they are distributed symmetrically in a first flow and second flow in the first and second discharge areas respectively when the ignition voltage is applied, in such a way that a first plasma and a second plasma are initiated simultaneously, respectively, in the first discharge area and in the second discharge area.. .
|Automatic gain control of a received signal using a power target|
The present invention relates to a method and device for performing automatic gain control of a received signal. The method comprises the steps of receiving (s101) the signal and amplifying the received signal on the basis of a difference between a power reference value and actual power of the amplified signal.
|Amplifier circuit and amplification method|
A true ground amplifier circuit in which a voltage sensor senses the output voltage and generates a binary output which indicates whether the output is above or below a threshold. A variable gain feedback system generates a feedback signal for combination with the digital input, thereby to provide offset cancellation.
|Devices and methods for monitoring and controlling temperature in a microfluidic environment|
The present invention provides improved methods that allow accurate monitoring and/or control of temperature changes in a microfluidic environment. An advantage of the present invention is that the temperature can be monitored and/or controlled at any location within a microfluidic device, especially where a preparation step, an amplification step and/or a detection step is performed.
Disclosed is a thermoacoustic engine having: resonance pipes including a working gas; motors; and a branch pipe, where each of the motors has a regenerator, a heater, and a cooler, a temperature gradient is given between both ends of the regenerator to generate self-excited oscillation of the working gas, a channel cross-sectional area of the resonance pipe that is coupled to the heater is expanded by a same amplification factor of a work flow based on the self-excited oscillation or by an amplification factor within a range of ±30% of the amplification factor of the work flow to a channel cross-sectional area of a resonance pipe that is coupled to the cooler, and a channel cross-sectional area of the regenerator is set by 4 to 36 times of the channel cross-sectional area of the resonance pipe that is coupled to the cooler.. .
|System and method for outcome tracking and analysis|
Provided are systems and methods for capturing outcome information associated with various cancer treatments. The system facilitates capture and analysis of cancer treatment information and associated outcome information.
|Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes|
Provided are devices comprising multivolume analysis regions, the devices being capable of supporting amplification, detection, and other processes. Also provided are related methods of detecting or estimating the presence nucleic acids, viral levels, and other biological markers of interest..
|Method and kit for characterizing rna in a composition|
V. Sequencing the amplification products by means of next generation sequencing..
|Apparatus for regulating transmission power of repeater in long term evolution system|
The present invention relates to an apparatus capable of flexibly regulating coverage of a repeater in a long term evolution system (hereinafter referred to as an lte system). The present invention provides an apparatus for regulating transmission power of a repeater in an lte (long term evolution) system, the apparatus including a sink signal detector that detects a sink signal of a reception signal received through an if/rf receiver of the repeater, a sink signal power measurer that measures a size of power of the sink signal detected through the sink signal detector, and an amplification controller that calculates a gain of an amplifier of the repeater for maintaining coverage of the repeater based on the size of the power of the sink signal measured through the sink signal power measurer, and controls the amplifier of the repeater according to the calculated gain.
|Method for signal amplification in biosensor-based system for rapidly detecting infectious agents|
A system for detecting infectious agents in biological samples in real time that includes a sample to be tested for at least one specific infectious agent; and a biosensor, wherein the biosensor is operative to detect a specific infectious agent in the sample to be tested; and wherein the biosensor emits an amplified and detectable signal when it reacts with the specific infectious agent. Signal detection is enhanced through the release of lps from the cells of the infectious agent..
|Apparatus for detecting nucleic acid amplification product in real time|
There is provided an apparatus for detecting a nucleic acid amplification product in real time, which is capable of effectively excluding or reducing apparatus error factors without using a second fluorescence signal used for correction. A plurality of wells 7a are given with temperature cycles and fluorescence strength from a nucleic acid amplification product is detected in real time in each well 7a.
|Analyte enrichment methods and compositions|
Methods and compositions for enriching a population of particles containing an analyte are disclosed. In one embodiment, enrichment beads are used that are larger in size than the beads used for amplification.
|Direct nucleic acid analysis|
The present disclosure provides methods, systems, and apparatuses for collecting and/or amplifying nucleic acids. In general, provided methods, systems, and apparatuses involve contacting a sample including a nucleic acid with a nucleic acid amplification reagent without purification of nucleic acids from the sample..
|Methods for detecting nucleic acid sequence variants|
The present invention provides methods for detecting the presence or absence of a nucleic acid variant in a target region. These methods include amplifying the target region with a forward primer and a reverse primer in the presence of a selector blocker.
|Dual bridge amplifier configuration|
An amplifier has a dual bridge design with two bridge amplifiers. A mode switch enables them to be configured in a series amplification mode.
|Methods and apparatus for all digital multi-level outphasing using bandwidth reduction|
For use in a transmitter in a wireless network, a transmitter apparatus is configured for digital multi-level outphasing. The apparatus includes a bandwidth reduction (bwr) modulator block configured to receive a phase modulated carrier and reduce a bandwidth of the phase modulated carrier using amplitude modulation.
|Method and device for the optical parametric amplification of pulses with frequency drift|
According to the invention, which is particularly applicable to laser-matter interaction, a four-wave mixing effect is used, preferably in an optical fibre (f), between each pulse (s) and the two pump signals (p1, p2); and the half-sum (fm) of the respective optical frequencies (fp1, fp2) of these signals pertains to the pulse spectrum support.. .
|Surface plasmon resonance biosensor|
An optical biosensor is provided for detecting a bio-molecular sample by goos-hänchen (gh) enhancement of aharonov-albert-vaidman (aav) amplification to a surface plasmon resonance (spr) detector. The sensor includes pre- and post-selection polarizers respectively upstream and downstream of a right-isosceles prism with a metal film and a liquid medium disposed on a diagonal side of the prism.
|Optical parametric amplification of weak signals for imaging biological tissue|
Methods and apparatus for obtaining an image of light scattering biological tissue. A series of pulses of substantially monochromatic light of a first wavelength is generated and split into two parts, of which one part illuminates a scattering biological tissue at an intensity too low to damage the tissue, while a second part is upconverted to generate a pump beam.
|Photon counting controller, radiographic imaging apparatus, and control method of photon counting controller|
The photon counting controller includes an amplification controller to charge an input electrical signal, to amplify the input electrical signal, and to discharge the charged electrical signal, a charge controller to control a charge and a discharge of the electrical signal of the amplification controller based on a received feedback signal, and a measuring controller to compare voltage of the amplified electrical signal with reference voltage and to count photons based on a result of comparison.. .
|Laser scanning code symbol reading system|
A laser scanning symbol reading system includes an analog scan data signal processor for producing digital data signals, wherein during each scanning cycle, a light collection and photo-detection module generates an analog scan data signal corresponding to a laser scanned symbol, a multi-channel parallel scan data signal processor/digitizer processes the analog scan data signal along multiple cascaded multi-stage signal processing channels, to generate digital data signals corresponding thereto, while a synchronized digital gain control module automatically processes the digital data signals in response to start of scan (sos) signals generated by a sos detector. Each signal processing channel supports different stages of amplification and filtering using a different set of band-pass filtering and gain parameters in each channel, to produce multiple digital first derivative data signals, and/or multiple digital scan data intensity data signals, having different signal amplitudes and dynamic range characteristics for use in decode processing..
|Electromagnetic wave treatment method and promoter|
A method involving exposing a substance to a promoter, the promoter being made from a liquid carrier and a metal salt component, the metal salt component, having at least (i) a magnetic susceptibility above 1000 or (ii) an ionization potential below about 500 volts, or (iii) both (i) and (ii), and applying electromagnetic wave energy to the substance while the substance is in the presence of the promoter, for a period of time and at a frequency and amplification sufficient to promote the modification of at least one physical property of the substance. A related method of treating a well in a subterranean formation, a tank or a pipeline, and the well treatment promoter composition, are also described..
|Method of amplifying a nucleic acid|
A nucleic acid amplification method is provided, along with kits useful in performing the amplification method.. .
|Constructing method of high-throughput sequencing library and use thereof|
The present invention provides a method for constructing a high-throughput sequencing library, which comprises: fragmenting genomic dna; end-repairing the dna fragments; adding a base a to the 3′ end of the end-repaired dna fragments; connecting the dna fragments having cohesive end a with a methylated adapter; carrying out hybrid capture on the connection products by using specific probes to obtain object fragments; treating the object fragments with bisulfite, to convert non-methylated cytosines to uracils; pcr amplifying the converted object fragments; and separating and purifying the amplification products, wherein the amplification products constitute the high-throughput sequencing library. The present invention also provides a method and an apparatus for identifying methylation information in specified genome regions of a sample..
|Devices, methods, and systems for nucleic acid amplification|
A microfluidic device comprising a first surface portion and a first sample retaining element, which have differing affinities to a fluid, and a method comprising supplying a sample to such a device. In some embodiments, the differing affinity is a result of plasma, ion embedding, surface charging, chemical, optical, electronic and/or electromagnetic treatment.
|Multiplexed diagnostic detection apparatus and methods|
Disclosed herein are devices configured for the amplification and detection of multiple targets from a sample, and methods of using the same. The devices disclosed herein comprise microfluidic cartridges have a first stage (amplification) and a second (detection) stage.
|Biosensor using redox cycling|
The present invention relates to a biosensor using dual amplification of signal amplification by means of enzymes coupled with signal amplification by means of redox cycling, and to the use of a technology that maintains a slow reaction state between redox cycling materials without the use of redox enzymes, and induces quick chemical-chemical redox cycling. In addition, the present invention relates to a biosensor which obtains triple amplification by inducing electrochemical-chemical-chemical redox cycling, in addition to signal amplification by means of enzymes and signal amplification by means of chemical-chemical redox cycling..
|Methods for multiplexing amplification reactions|
A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step.
|Amplification methods to minimise sequence specific bias|
Methods for amplifying nucleic acids are provided. The methods can be used to minimise sequence specific bias caused by the preferential amplification of certain nucleic acid sequences.
|Apparatus for amplification of nucleic acids|
Described herein is a chip-based apparatus for amplifying nucleic acids, a cartridge housing the apparatus, and methods of using the apparatus for amplification of nucleic acids. More specifically, this invention provides integrated semiconductor chip, manufactured with standard semiconductor manufacturing process, with on-chip circuitry to perform thermal management and optical sensing necessary for amplification of nucleic acids.
|Chip-based sequencing nucleic acids|
A system for fast dna sequencing by amplification of genetic material within microreactors, denaturing, demulsifying, and then sequencing the material, while retaining it in a pcr/sequencing zone by a magnetic field. One embodiment includes sequencing nucleic acids on a microchip that includes a microchannel flow channel in the microchip.
|Method for the detection of nucleic acid synthesis and/or amplification|
The present invention relates to a method for the detection of nucleic acid synthesis and/or amplification, characterised in that the method includes adding at least one colorimetric metal indicator and at least one bland magnesium chelator to a reaction mixture for nucleic acid amplification. The present invention further relates to a kit for carrying out such a method and to the use thereof in the health, food and agricultural or veterinary fields..