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Amplification patents



      
           
This page is updated frequently with new Amplification-related patent applications. Subscribe to the Amplification RSS feed to automatically get the update: related Amplification RSS feeds. RSS updates for this page: Amplification RSS RSS


Molecular nets on solid phases

Molecular nets on solid phases

Date/App# patent app List of recent Amplification-related patents
10/23/14
20140317773
 Yield traits for maize patent thumbnailYield traits for maize
Methods for introgressing an allele of interest of a locus associated with a yield trait into zea mays germplasm are provided. In some embodiments, the methods include providing a zea mays plant that contains an allele of interest of a locus associated with a yield trait, wherein the locus associated with the yield trait is identifiable by pcr amplification of a zea mays nucleic acid with a pair of oligonucleotides primers as disclosed herein, and introgressing the allele of interest into zea mays germplasm that lacks the allele.
10/23/14
20140315759
 Molecular nets on solid phases patent thumbnailMolecular nets on solid phases
Disclosed is a covalently-linked multilayered three-dimensional matrix comprising capture molecules, linkers and spacers (referred to as a molecular net) for specific and sensitive analyte capture from a sample. Also disclosed herein is a molecular net comprising covalently-linked multilayered three-dimensional matrix comprising more than one type of capture molecule and more than one type of linker and may comprise one or more spacer for specific and sensitive capture of more than one type of analyte from a sample.
10/23/14
20140315750
 Selective detection of norovirus patent thumbnailSelective detection of norovirus
A process for detecting norovirus nucleic acid in a sample is provided including producing an amplification product by amplifying a norovirus nucleotide sequence using a forward primer of seq id no: 1, 4, or 10 and a reverse primer of seq id no: 2, 5, or 11, and detecting the amplification product to detect norovirus in the sample. Also provided are reagents and methods for detecting and distinguishing gi or gil norovirus from other infectious agents.
10/23/14
20140315745
 Broad detection of dengue virus serotypes patent thumbnailBroad detection of dengue virus serotypes
Processes for detecting dengue virus (denv) nucleic acid in a sample are provided including producing an amplification product by amplifying denv nucleotide sequence and detection of an amplification by hybridization of a probe or other technique. The processes use primers or probes with introduced mutations and or degenerate bases that provide excellent superiority in detection and serotyping of denv in a sample..
10/23/14
20140315729
 Detection of nucleic acid reactions on bead arrays patent thumbnailDetection of nucleic acid reactions on bead arrays
The present invention is directed to methods and compositions for the use of micro sphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e.
10/23/14
20140315728
 Method for high-throughput aflp-based polymorphism detection patent thumbnailMethod for high-throughput aflp-based polymorphism detection
The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of dna, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.. .
10/23/14
20140315642
 Gaming device having a haptic-enabled trigger patent thumbnailGaming device having a haptic-enabled trigger
A haptic peripheral comprising a housing, a user input element, a position sensor coupled to the user input element, and an actuator located within the housing and coupled to the user input element. The position sensor is configured to detect a position of the user input element and is configured to send the position to a processor.
10/23/14
20140315503
 Multiple input single output device with vector signal and bias signal inputs patent thumbnailMultiple input single output device with vector signal and bias signal inputs
Methods and systems for vector combining power amplification are disclosed herein. In one embodiment, a plurality of signals are individually amplified, then summed to form a desired time-varying complex envelope signal.
10/23/14
20140315325
 Assembly comprising a reaction vessel and a sample matrix, sample matrix comprising an upper adsorbent layer and a lower lateral flow layer patent thumbnailAssembly comprising a reaction vessel and a sample matrix, sample matrix comprising an upper adsorbent layer and a lower lateral flow layer
An assembly and related methods are described for the preparation and processing of samples in molecular biology assays and nucleic acid amplification reactions. The assembly allows rapid and easy processing with reduced sample handling compared to known sample preparation apparatus and methods.
10/23/14
20140315211
 Methods for suppression pcr patent thumbnailMethods for suppression pcr
Provided herein are approaches for the detection, identification, and/or selective amplification of specific target species or target variants of nucleic acid sequences, even within an excess of unwanted similar sequences or variants. These approaches include methods, assays, and kits for suppression pcr that require, in part, dna polymerase that lacks 5′-3′ exonuclease activity, and a pcr primer, termed a forward selective primer or a nunchaku primer.
10/23/14
20140315209
Molecular assay for the amplification and detection of kpc genes responsible for high-level resistance to carbapenem in gram negative bacteria
Methods and kits useful for the detection and identification of carbapenem-resistant pathogens harboring carbapenemase-encoding nucleic acids. Said methods comprise pcr amplification of a target region of the beta-lactamase encoding klebsiella pneumoniae carbapenemase genes (blakpc) and variants thereof with a primer set comprising seq id nos: 1 and 2, and variants thereof.
10/23/14
20140315204
Immunohistochemistry detection method
The invention provides compositions and methods for the detection of targets in a sample; in particular, an immunohistochemistry (ihc) sample. Probes and detectable labels may be provided in multiple layers in order to increase the flexibility of a detection system, and to allow for amplification to enhance the signal from a target.
10/23/14
20140315202
Method for assembling multiple target loci to single nucleic acid sequence
A method for assembling multiple target loci to a single assembled nucleic acid sequence involves assembling the multiple target loci to a single assembled nucleic acid sequence through two polymerase chain reactions (pcrs). A pair of primers for a primary amplification include a target-specific sequence and a 5′-flanking assembly spacer sequence.
10/23/14
20140315197
Multifunctional probe-primers
Methods and reagents for detection and analysis of nucleic acids are provided. Certain methods involves an encoding amplification in which a target sequence is associated with probe-binding sequences and optionally with indexing sequences, (2) an optional distribution step in which the product of the encoding amplification is split into multiple aliquots, and (3) a decoding and detection step in which the presence, absence, quantity, or relative amount of the target sequence in the aliquots is determined.
10/23/14
20140314372
Suppression of stimulated brillouin scattering in higher-order- mode optical fiber amplifiers
An hom-based optical fiber amplifier is selectively doped within its core region to minimize the presence of dopants in those portions of the core where the unwanted lower-order modes (particularly, the fundamental mode) of the signal reside. The reduction (elimination) of the gain medium from these portions of the core minimizes (perhaps to the point of elimination) limits the amount of amplification impressed upon the backward-propagating stokes wave.
10/23/14
20140314253
Vibration conduction and frequency-selective amplification device for a moving-iron microphone/transducer
A vibration conduction and frequency-selective amplification device for a moving-iron microphone/transducer, including connecting portions at both ends and a middle portion for vibration conduction and frequency-selective amplification. The device is mechanically connected to a microphone armature and a vibration diaphragm of the moving-iron microphone/transducer, and performs frequency-selective amplification on a vibration generated by the armature and transmits the vibration onto the diaphragm by the middle portion, so as to achieve frequency-selective amplification and conversion from mechanical energy to acoustic energy.
10/23/14
20140314106
Ultra-high power single mode fiber laser system with non-uniformly configure fiber-to-fiber rod multimode amplifier
A high power fiber laser system includes a booster winch is configured as a fiber amplifier extending over free space, pump source and laser head including a reflective element which receives pump light and reflects toward the output end of the booster in a counter signal-propagating direction. The booster is configured with concentric and coextending frustoconically shaped (“mm”) core and cladding around the core.
10/23/14
20140313510
Multiplex fluorescent particle detection using spatially distributed excitation
A chip-scale optical approach to performing multi-target detection is based on molecular biosensing using fiber-optic based fluorescence or light scattering detection in liquid-core waveguides. Multiplexing methods are capable of registering individual nucleic acids and other optically responsive particles, and are ideal for amplification-free detection in combination with the single molecule sensitivity of optofluidic chips.
10/23/14
20140312971
Differential amplifier circuit
A differential amplifier circuit includes a differential amplification unit suitable for amplifying difference between signals of an input terminal and a complementary input terminal, receiving the same voltage level through the input terminal and the complementary input terminal at a measurement period, and receiving an input signal and a complementary input signal through the input terminal and the complementary input terminal, respectively, at an operation period, an offset control unit suitable for generating offset information using an output of the differential amplification unit at the measurement period, and an offset compensation unit suitable for compensating for an offset of the differential amplification unit in response to the offset information.. .
10/23/14
20140312970
Power supply circuit
A power supply circuit includes: a push-pull amplifier unit which amplifies an input signal with a push-pull amplification protocol; a variable power supply unit which varies, with a control signal, the voltage level of a power supply voltage which is supplied to the push-pull amplifier; a switch control unit which, on the basis of the input signal, outputs the control signal which controls the voltage level of the power supply voltage; and a timing control unit which applies a specified time delay to the input signal. When the control signal rises, the switch control unit causes the control signal to rise at a faster timing, according to the voltage level switch transition timing, than a delay time upon the timing control unit, and when the control signal falls, the switch control unit causes the control signal to fall at the timing of the delay time..
10/23/14
20140312867
Low drop out voltage regulator and related method of generating a regulated voltage
A low drop out voltage regulator includes an operational transconductance amplifier configured to be supplied with a supply voltage of the regulator, receive as inputs a reference voltage and a feedback voltage, and generate an intermediate current based upon a difference between the reference voltage and the feedback voltage. A current-to-voltage amplification stage is configured to be supplied with a boosted voltage greater than the supply voltage from a high voltage line, receive as input the intermediate current, and generate a driving voltage that is changed based upon the intermediate current.
10/23/14
20140311246
Tread thickness measuring method
A method for measuring the tread thickness from the radially outermost belt surface within a tire to the tread surface accurately by ultrasonic measurement whatever the type of belt material is. The method includes the steps of measuring a profile of the tread surface, identifying the bottom positions of circumferentially extending grooves from the profile measured, estimating the depths from the groove bottoms to the belt surface from the identified groove bottoms and pre-created tire design data, predicting thicknesses by calculating predicted thicknesses from the tread surface to the belt surface from the relationship between the thicknesses from the identified groove bottoms to the tread surface in the profile and the estimated depths from the groove bottoms to the belt surface, and setting amplification factors for received reflected waves according to a reflection factor-thickness data map prepared beforehand in correspondence to the predicated thicknesses..
10/23/14
20140311243
Estimation of sidewall skew angles of a structure
An apparatus (36) includes a motion amplification structure (52), an actuator (54), and a sense electrode (50) in proximity to the structure (52). The actuator (54) induces an axial force (88) upon the structure (52), which causes a relatively large amount of in-plane motion (108) in one or more beams (58, 60) of the structure (52).
10/16/14
20140309504
In-body device with virtual dipole signal amplification
Virtual dipole signal amplification for in-body devices, such as implantable and ingestible devices, is provided. Aspects of the in-body deployable antennas of the invention include antennas configured to go from a first configuration to a second configuration following placement in a living body, e.g., via ingestion or implantation.
10/16/14
20140309145
High throughput method for assembly and cloning polynucleotides comprising highly similar polynucleotidic modules
The present invention relates to a method for the assembly and cloning of polynucleotides comprising highly similar polynucleotidic modules, that is highly versatile, does not require intermediate amplification step and can be easily automated for high throughput production of customized polynucleotidic modules.. .
10/16/14
20140309143
Centroid markers for image analysis of high density clusters in complex polynucleotide sequencing
Improved compositions, methods, apparatus, and kits for high-throughput nucleic acid amplification, detection and sequencing are disclosed. A nucleic acid cluster having an identifiable center is produced by generating on a solid support an immobilized nucleic acid complement from a template, one of which comprises a detectable label; and amplifying the complement and the template to obtain a nucleic acid cluster on the support, the cluster having a substantially central location marked by the detectable label and a surrounding region comprising immobilized copies.
10/16/14
20140309138
Compositions and methods of detecting respiratory pathogens using nucleic acid probes and subsets of beads
The present disclosure is instructional for a multiplex nucleic acid amplification assay to detect and identify multiple respiratory pathogens.. .
10/16/14
20140309136
Real-time multiplex detection of three bacterial species responsible for sexually transmitted diseases
The invention relates to the detection of three different bacterial species which are responsible for sexually-transmitted diseases, i.e., chlamydia trachomatis (ct), neisseria gonorrhoeae (ng) and mycoplasma genitalium (mg). The invention more particularly relates to the detection of these three species in real-time pcr, in multiplex pcr and in real-time multiplex pcr.
10/16/14
20140309120
Method and substrate for nucleic acid amplification, and method and apparatus for nucleic acid analysis
The present invention relates to a method for nucleic acid amplification, which enables clusters of amplified nucleic acid fragments to be sequenced by a sequencer to be formed at a high density and improves throughput of nucleic acid sequence analysis by amplifying the number of nucleic acids in the cluster to 10,000 molecules or more; and a method for nucleic acid amplification for enhancing read accuracy, which achieves a high cluster density and increases the number of the amplified fragments in the cluster by the steps of previously forming a pattern of primer dnas on a base material and fixing bulky template dna molecules synthesized from dna samples thereon to induce amplification reaction.. .
10/16/14
20140309119
Methods and devices for high fidelity polynucleotide synthesis
Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products..
10/16/14
20140308908
Apparatus and method for signal predistortion
Apparatus and a method for predistortion of a radio-frequency signal are described. At least one predistortion unit is described that is arranged to receive an analog baseband signal before amplification and to generate an analog predistortion signal based on said analog baseband signal.
10/16/14
20140308673
Methods for nucleic acid manipulation
A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor.
10/16/14
20140308666
Development of novel detergents for use in pcr systems
This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (pcr). Methods for preparing the modified detergents are also described..
10/16/14
20140308663
Method of nucleic acid amplification and measuring reagent and reagent kit therefor
The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor..
10/16/14
20140308045
Pulsed light communication key
A universal serial bus (usb) key may include an optical transceiver having a usb interface for engagement to an electronic device such as a laptop computer or other usb-configured device. The usb key may include a converter or buffering, isolation, modulation or amplification circuitry.
10/16/14
20140307978
Method and system for analog/digital image simplification and stylization
A computer program product includes instructions for a processor to perform a method of improving image structure and coherence, and/or for simplification/stylization. The method includes inputting the image, by: scanning the image; image capture using a camera; and/or image creation using a computer.
10/16/14
20140307765
Wireless transmission system, and method for determining default gain of wireless transmission system
A method for determining a default gain of a wireless transmission system is provided. The wireless transmission system includes a signal transmission path and a signal feedback path coupled to the signal transmission path.
10/16/14
20140306798
Rfid system and communication method performed by the same
Provided is an rfid system and method for maintaining a constant strength of a signal transmitted from an rfid tag to an rfid reader, regardless of a distance between the reader and tag. The rfid signal strength measuring reader measures a signal strength received from the rfid tag, creates control information used to set an amplification amount of the rfid tag by using a value of the measured strength to include the control information in an output signal, and sends the output signal to the rfid tag.
10/16/14
20140306769
Dual instantaneous envelope tracking
Power supply circuitry, which includes a parallel amplifier and a parallel amplifier power supply, is disclosed. The power supply circuitry operates in either an average power tracking mode or an envelope tracking mode.
10/16/14
20140306765
Doherty power amplifier, and method and device for improving power amplification efficiency of doherty power amplifier
One aspect of the present invention provides a method for improving power amplification efficiency of a doherty power amplifier. The method is applied to a doherty power amplifier that has two paths of doherty circuit units connected in parallel.
10/16/14
20140306758
Versatile audio power amplifier
An audio power amplifier includes a first and a second amplification unit, each including a switching voltage amplifier, an output filter, a current compensator, an inner current feedback loop feeding a measurement of current measured at the output inductor back to a summing input of the current compensator, a voltage compensator coupled to the summing input of the current compensator, and an outer voltage feedback loop. A controlled signal path provides the output of the voltage compensator of the first amplification unit to the current compensator of the second amplification unit.
10/16/14
20140306111
Low temperature co-fired ceramic system on package for millimeter wave optical receiver and method of fabrication
The present disclosure relates to a multi-layered low temperature co-fired ceramic (ltcc) system on package (sop) for a millimeter wave optical receiver comprising a top layer, a plurality of first intermediate layers, a plurality of second intermediate layers, and a bottom layer. The top layer further comprises a matching network, passive components, and a signal line disposed on a substrate material, the plurality of first intermediate layers further comprises active amplification components, via holes and a plurality of inner grounding planes that are respectively disposed on a first plurality of ltcc substrates, the plurality of second intermediate layers further comprises a plurality of grounding planes that are respectively disposed on a second plurality of ltcc substrates; and the bottom layer further comprises a grounding plane that is disposed on the bottom surface of the second plurality of ltcc substrates.
10/09/14
20140304575
Digital-analog conversion apparatus and method
An apparatus and a method for digital-analog conversion are provided. The apparatus includes a first cell matrix for outputting a current of a signal corresponding to a number of most significant bits (msbs) of an input digital signal, a second cell matrix for outputting a current of a signal corresponding to a number of least significant bits (lsbs) of the input digital signal, an amplifier for amplifying the output current of the second cell matrix at a preset amplification, and an adder for adding the output current of the first cell matrix and the output current of the amplifier..
10/09/14
20140303038
3' biased detection of nucleic acids
The invention provides materials and methods for the detection of nucleic acid expression via the 3′ portion of expressed sequences. Embodiments of the invention include the use of microarrays comprising nucleic acid probes that are complementary to the 3′ end of expressed sequences and by the use of quantitative pcr (q-pcr) based amplification of sequences found at or near the 3′ end of expressed sequences.
10/09/14
20140303017
Multiplexed amplification of short nucleic acids
The present teachings provide methods, compositions, and kits for reverse transcribing and amplifying small nucleic acids such as micro rnas. High levels of multiplexing are provided by the use of a zip-coded stem-loop reverse transcription primer, along with a pcr-based pre-amplification reaction that comprises a zip-coded forward primer.
10/09/14
20140303000
Compositions and methods for directional nucleic acid amplification and sequencing
The invention provides methods and compositions, including kits, for directional nucleic acid amplification and sequencing. The invention further provides methods and compositions for the construction of directional cdna libraries..
10/09/14
20140302776
Automatic gain correction circuit with variable setpoint
An automatic gain correction circuit for radiofrequency signals applies notably to the regulation of the amplification of signals for satellite radionavigation. The automatic gain correction circuit is able to receive an input radiofrequency signal and to deliver an output radiofrequency signal of which a mean amplitude is slaved to a setpoint.
10/09/14
20140302505
Methods and systems to analyze reactions using an information system
Disclosed are example methods and systems to determine the quantity of an analyte initially present in a chemical and or biological reaction. Also disclosed are computer implemented methods and systems to automate portions of the analysis comprising mathematical or graphical analysis of an amplification reaction..
10/09/14
20140302504
Nucleic acid amplification
Methods and compositions for the amplification of nucleic acids are disclosed. Amplification methods provided herein may be performed under isothermal conditions.
10/09/14
20140302500
Compositions and methods for detecting bv-associated bacterial nucleic acid
Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of a 16s rrna or its encoding gene from bacterial species associated with bacterial vaginosis. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits..
10/09/14
20140300887
Optical distance measuring apparatus
An optical distance measuring apparatus including a light source, a variable mirror scanning the light on an object, a receiving device receiving light from the object, a detected signal amplifying unit detecting a light receiving signal obtained by the receiving device, an amplification control unit detecting a set target value of light sensitivity of the receiving device or sets the light sensitivity based on a light quantity of reflected light, and a distance calculation unit detecting a flight time of the ranging light from a light emitting signal and calculating a distance up to the object, wherein in a first scan period, the amplification control unit detects the set target value based on a light quantity of the reflected light, and in a second scan period, the amplification control unit sets sensitivity of the receiving device to the set target value and the distance calculation unit calculates the distance.. .
10/09/14
20140300435
Electromagnetic actuator
There is provided an electromagnetic actuator which can secure a sufficient thrust force at least at a certain level over a wide range of displacement. The electromagnetic actuator 1 having a point of amplified displacement includes: a displacement amplification mechanism 1a made of a magnetic material and having two surfaces 2as, 2bs that form a gap 5 therebetween; and a coil 6 provided in the displacement amplification mechanism 1a.
10/09/14
20140298787
Pulsed hydraulic pressure amplification system
A hydraulic pressure amplification system for increasing the output pressure in the delivery pipe to or from a ram pump, a spring rebound inertia pump or similar cyclic pumps which deliver a pulsating flow. Said hydraulic pressure amplification system comprises a fluid inlet (29), a fluid outlet (30) and one or more rigid bodies (21) which contain an enclosed convolute passageway extending between the fluid inlet and the fluid outlet.
10/02/14
20140296723
Heartbeat signal processing method
A heartbeat signal method includes detecting first and second heartbeat signals, where the first and second heartbeat signals may be potential difference signals with respect to a reference potential. First and second average dc voltage values as voltage values of direct-current components in the first and second heartbeat signals are calculated.
10/02/14
20140296100
Methods for treating cancer resistant to erbb therapeutics
Provided herein are methods for treating cancer that is resistant to treatment with an anti-erbb therapeutic agent and which is associated with an activating met gene mutation or a met gene amplification. The methods involve administering to a subject a combination of an anti-erbb therapeutic and an anti-met therapeutic.
10/02/14
20140296084
Method for preparing nucleic acid library, its uses and kits
Provided are a method of constructing a nucleic acid library, a method of determining a nucleic acid sequence of a nucleic acid sample, and a kit thereof. The method of constructing the nucleic acid library includes the following steps: subjecting a nucleic acid sample to a dop-pcr amplification, to obtain a first pcr amplification product; subjecting the first pcr amplification product to a second pcr amplification using a dop-amp primer, to obtain a second pcr amplification product; and subjecting the second pcr amplification product to an adaptor-ligation pcr, to obtain a third pcr amplification product, wherein the third pcr amplification product constitutes the nucleic acid library..
10/02/14
20140296006
System and method for a game racquet including an actuator
The present invention provides a game racquet including an integrated circuit capable of supporting a low-voltage energy current. An embodiment of the invention provides a game racquet including one or more enhancements attached or integrated on the racquet frame.
10/02/14
20140295498
Targeted rolling circle amplification
Methods for amplifying a desired target region of a nucleic acid through rolling circle amplification with a strand-displacing polymerase are provided. Concatameric hairpin products are resolved with endonuclease digestion, and the resulting amplified product hairpins or fragments can be circularized and employed as templates in a subsequent round of amplification.
10/02/14
20140295492
Methods for cell-free protein synthesis
Cell-free protein synthesis systems and methods of using the same for producing in vitro protein materials in high yield are disclosed. The cell-free protein synthesis platform includes (a) a saccharomyces cerevisiae cellular extract prepared from mid-exponential to late-exponential batch cultures in the range from about 6 od600 to about 18 od600 or fed-batch cultures harvested in mid-exponential to late-exponential phase; (b) a reaction buffer; and (c) a translation template or (c′) a transcription template from which a translation template can be prepared in situ with an rna polymerase.
10/02/14
20140295447
Primer set, method for amplifying target nucleic acid sequence using same, and method for detecting mutated nucleic acid using same
The present invention provides a primer set including primers that can be designed easily, with which an amplification distance can be shortened. Provided is a primer set for use in a method for isothermally amplifying a target nucleic acid sequence 4.
10/02/14
20140295445
Method for the carry-over protection in dna amplification systems targeting methylation analysis achieved by a modified pre-treatment of nucleic acids
Particular aspects provide methods for specific amplification of template dna in the presence of potentially contaminating pcr products from previous amplification experiments. Particular embodiments comprise, in a first step, contacting dna with a bisulfite solution, which sulfonates unmethylated (but not methylated) cytosines, resulting in cytosine deamination and generation of sulfonated uracil.
10/02/14
20140295441
Cartridge interface module
A cartridge interface module (cim), configured to engage with a removable microfluidic cartridge in a nucleic acid analyzer system can include a fluidics component, which is configured to initiate and support a liquid extraction of nucleic acids from a biological sample contained in the removable microfluidic cartridge. The cim also includes a polymerase chain reaction (pcr) assembly component which can be configured to initiate and support amplification of the extracted nucleic acids.
10/02/14
20140295440
Nucleic acid amplification
Methods and compositions for the amplification of nucleic acids and generation of concatemers are disclosed. Amplification methods provided herein may be performed under isothermal conditions.
10/02/14
20140295439
Nucleic acid amplification
Methods and compositions for the amplification of nucleic acids are disclosed. Amplification methods provided herein may be performed under isothermal conditions.
10/02/14
20140295438
Method and components for detecting nucleic acid chains
The invention relates to a novel method for the enzymatic marking of nucleic acid chains (target sequences) by means of nucleotide conjugates. Said nucleotide conjugates are able, under reaction conditions, to specifically bind to the target sequence and integrate into the complementary growing strand by means of a polymerase.
10/02/14
20140295436
Methods for multiplexing recombinase polymerase amplification
This disclosure provides for methods and reagents for rapid multiplex rpa reactions and improved methods for detection of multiplex rpa reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between rpa processes..
10/02/14
20140295431
Method of allele-specific amplification
A method of selectively producing and amplifying a cdna sequence of a target allele of a gene, wherein the target allele is a mutant allele or is a specific allele of a polymorphic gene, the method comprising: (a) providing a sample comprising an mrna transcript of the target allele; (b) performing a reverse-transcription reaction to generate a cdna sequence from the mrna transcript, and (c) amplifying the cdna of the target allele; wherein the reverse-transcription reaction is selective for reverse transcription of the mrna transcript of the target allele over an mrna transcript of an alternative allele of the same gene.. .
10/02/14
20140295428
Method for high-throughput aflp-based polymorphism detection
The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of dna, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.. .
10/02/14
20140295419
Methods and compositions for isothermal whole genome amplification
Disclosed are methods and compositions for amplification of genetic material, including isothermal wga of single cells.. .
10/02/14
20140295417
Identification of antibiotic resistance
The invention relates to a method and a kit for the detection of an antibiotic resistance in a predetermined micro-organism in a biological sample. The method according to the invention comprises the following steps: (a) contacting the biological sample with a first nucleic acid labelled with a first label and capable of selectively hybridizing with a nucleic acid in the micro-organism, (b) identifying the micro-organism by the detection of the presence of the first label in an individual cell of the micro-organism, (c) contacting the sample with at least one probe for detection of an antibiotic resistance in a micro-organism, wherein said at least one probe is labelled with at least a second label, and (d) determining the antibiotic resistance of the micro-organism by the detection of the presence of at least the second label, wherein steps (b) and (d) are performed simultaneously so as to allow quick identification of a pathogen directly from a sample without culturing and without prior amplification..


Popular terms: [SEARCH]

Amplification topics: Amplification, Nucleic Acid, Nucleotide, High Resolution, Modulation, Feedback Signal, Nanoparticle, Semiconductor, Raman Amplifier, Resonance Frequency, User Interface, Transmitter, Methylation, Sample Preparation, Sequencing

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This listing is a sample listing of patent applications related to Amplification for is only meant as a recent sample of applications filed, not a comprehensive history. There may be associated servicemarks and trademarks related to these patents. Please check with patent attorney if you need further assistance or plan to use for business purposes. This patent data is also published to the public by the USPTO and available for free on their website. Note that there may be alternative spellings for Amplification with additional patents listed. Browse our RSS directory or Search for other possible listings.
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